NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 593, 827 |
| AciI | CCGC | 1 cut(s) | 659 |
| AclWI | GGATC | 2 cut(s) | 262, 404 |
| AcsI | RAATTY | 2 cut(s) | 525, 1108 |
| AcuI | CTGAAG | 7 cut(s) | 581, 716, 760, 881, 969, 1161, 1233 |
| AfaI | GTAC | 5 cut(s) | 122, 219, 246, 968, 1002 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 733, 1045 |
| AgsI | TTSAA | 9 cut(s) | 64, 203, 211, 224, 278, 557, 844, 992, 1161 |
| AjnI | CCWGG | 1 cut(s) | 144 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 151, 183 |
| AluBI | AGCT | 4 cut(s) | 563, 643, 730, 852 |
| AluI | AGCT | 4 cut(s) | 563, 643, 730, 852 |
| Alw21I | GWGCWC | 3 cut(s) | 378, 565, 625 |
| Alw26I | GTCTC | 4 cut(s) | 169, 254, 492, 908 |
| Alw44I | GTGCAC | 1 cut(s) | 621 |
| AlwI | GGATC | 2 cut(s) | 262, 404 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 739 |
| AoxI | GGCC | 1 cut(s) | 1008 |
| ApaLI | GTGCAC | 1 cut(s) | 621 |
| ApeKI | GCWGC | 5 cut(s) | 22, 152, 643, 856, 1031 |
| ApoI | RAATTY | 2 cut(s) | 525, 1108 |
| AspLEI | GCGC | 1 cut(s) | 166 |
| AsuHPI | GGTGA | 2 cut(s) | 439, 715 |
| AsuII | TTCGAA | 1 cut(s) | 170 |
| BaeGI | GKGCMC | 2 cut(s) | 625, 802 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 950, 983 |
| BanII | GRGCYC | 1 cut(s) | 565 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 984, 1016 |
| BauI | CACGAG | 1 cut(s) | 582 |
| Bbv12I | GWGCWC | 3 cut(s) | 378, 565, 625 |
| BbvI | GCAGC | 5 cut(s) | 9, 139, 630, 843, 1018 |
| BccI | CCATC | 1 cut(s) | 179 |
| BceAI | ACGGC | 1 cut(s) | 148 |
| BciT130I | CCWGG | 1 cut(s) | 146 |
| BclI | TGATCA | 2 cut(s) | 265, 765 |
| BcoDI | GTCTC | 4 cut(s) | 169, 254, 492, 908 |
| BfaI | CTAG | 2 cut(s) | 1167, 1220 |
| BfmI | CTRYAG | 2 cut(s) | 962, 1056 |
| BfuAI | ACCTGC | 2 cut(s) | 593, 827 |
| BisI | GCNGC | 6 cut(s) | 23, 153, 644, 660, 857, 1032 |
| BlpI | GCTNAGC | 1 cut(s) | 377 |
| BlsI | GCNGC | 6 cut(s) | 24, 154, 645, 661, 858, 1033 |
| BmcAI | AGTACT | 1 cut(s) | 219 |
| Bme1390I | CCNGG | 1 cut(s) | 146 |
| BmrFI | CCNGG | 1 cut(s) | 146 |
| BmsI | GCATC | 4 cut(s) | 352, 970, 1018, 1222 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 383, 415 |
| BpmI | CTGGAG | 1 cut(s) | 659 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 377 |
| Bpu14I | TTCGAA | 1 cut(s) | 170 |
| BpuEI | CTTGAG | 1 cut(s) | 554 |
| Bsa29I | ATCGAT | 2 cut(s) | 464, 1252 |
| BsaI | GGTCTC | 1 cut(s) | 908 |
| BsaJI | CCNNGG | 1 cut(s) | 145 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 733, 1045 |
| Bse1I | ACTGG | 3 cut(s) | 440, 512, 642 |
| Bse3DI | GCAATG | 3 cut(s) | 191, 372, 1101 |
| BseBI | CCWGG | 1 cut(s) | 146 |
| BseCI | ATCGAT | 2 cut(s) | 464, 1252 |
| BseDI | CCNNGG | 1 cut(s) | 145 |
| BseGI | GGATG | 3 cut(s) | 748, 813, 937 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 733, 1045 |
| BseMI | GCAATG | 3 cut(s) | 191, 372, 1101 |
| BseMII | CTCAG | 4 cut(s) | 26, 391, 528, 1035 |
| BseNI | ACTGG | 3 cut(s) | 440, 512, 642 |
| BseRI | GAGGAG | 2 cut(s) | 401, 404 |
| BseSI | GKGCMC | 2 cut(s) | 625, 802 |
| BseXI | GCAGC | 5 cut(s) | 9, 139, 630, 843, 1018 |
| BseYI | CCCAGC | 1 cut(s) | 726 |
| BsgI | GTGCAG | 1 cut(s) | 693 |
| BshFI | GGCC | 1 cut(s) | 1010 |
| BshVI | ATCGAT | 2 cut(s) | 464, 1252 |
| BsiHKAI | GWGCWC | 3 cut(s) | 378, 565, 625 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 733, 1045 |
| BsmAI | GTCTC | 4 cut(s) | 169, 254, 492, 908 |
| BsnI | GGCC | 1 cut(s) | 1010 |
| Bso31I | GGTCTC | 1 cut(s) | 908 |
| Bsp119I | TTCGAA | 1 cut(s) | 170 |
| Bsp1286I | GDGCHC | 4 cut(s) | 378, 565, 625, 802 |
| Bsp143I | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 377 |
| BspACI | CCGC | 1 cut(s) | 659 |
| BspANI | GGCC | 1 cut(s) | 1010 |
| BspCNI | CTCAG | 4 cut(s) | 25, 390, 527, 1036 |
| BspDI | ATCGAT | 2 cut(s) | 464, 1252 |
| BspHI | TCATGA | 1 cut(s) | 300 |
| BspMAI | CTGCAG | 1 cut(s) | 1060 |
| BspMI | ACCTGC | 2 cut(s) | 593, 827 |
| BspPI | GGATC | 2 cut(s) | 262, 404 |
| BspT104I | TTCGAA | 1 cut(s) | 170 |
| BspTNI | GGTCTC | 1 cut(s) | 908 |
| BsrDI | GCAATG | 3 cut(s) | 191, 372, 1101 |
| BsrI | ACTGG | 3 cut(s) | 440, 512, 642 |
| BssECI | CCNNGG | 1 cut(s) | 145 |
| BssMI | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| BssSI | CACGAG | 1 cut(s) | 582 |
| Bst2BI | CACGAG | 1 cut(s) | 582 |
| Bst2UI | CCWGG | 1 cut(s) | 146 |
| Bst4CI | ACNGT | 4 cut(s) | 671, 697, 883, 966 |
| Bst6I | CTCTTC | 2 cut(s) | 167, 583 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 22, 980, 1055, 1064 |
| BstBI | TTCGAA | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 2 cut(s) | 621, 854 |
| BstDEI | CTNAG | 5 cut(s) | 12, 377, 494, 514, 1044 |
| BstF5I | GGATG | 3 cut(s) | 748, 813, 937 |
| BstHHI | GCGC | 1 cut(s) | 166 |
| BstKTI | GATC | 6 cut(s) | 257, 268, 412, 768, 1022, 1252 |
| BstMAI | GTCTC | 4 cut(s) | 169, 254, 492, 908 |
| BstMBI | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 22, 141, 161, 736, 862, 980, 1055, 1064 |
| BstNI | CCWGG | 1 cut(s) | 146 |
| BstNSI | RCATGY | 1 cut(s) | 182 |
| BstSCI | CCNGG | 1 cut(s) | 144 |
| BstSFI | CTRYAG | 2 cut(s) | 962, 1056 |
| BstSLI | GKGCMC | 2 cut(s) | 625, 802 |
| BstV1I | GCAGC | 5 cut(s) | 9, 139, 630, 843, 1018 |
| Bsu15I | ATCGAT | 2 cut(s) | 464, 1252 |
| BsuRI | GGCC | 1 cut(s) | 1010 |
| BsuTUI | ATCGAT | 2 cut(s) | 464, 1252 |
| BtsCI | GGATG | 3 cut(s) | 748, 813, 937 |
| BtsIMutI | CAGTG | 4 cut(s) | 522, 676, 888, 1128 |
| BveI | ACCTGC | 2 cut(s) | 593, 827 |
| Cac8I | GCNNGC | 2 cut(s) | 621, 854 |
| CaiI | CAGNNNCTG | 1 cut(s) | 739 |
| CciI | TCATGA | 1 cut(s) | 300 |
| CfoI | GCGC | 1 cut(s) | 166 |
| ClaI | ATCGAT | 2 cut(s) | 464, 1252 |
| CseI | GACGC | 1 cut(s) | 1107 |
| Csp6I | GTAC | 5 cut(s) | 121, 218, 245, 967, 1001 |
| CviAII | CATG | 8 cut(s) | 48, 179, 301, 382, 490, 566, 769, 1006 |
| CviQI | GTAC | 5 cut(s) | 121, 218, 245, 967, 1001 |
| DdeI | CTNAG | 5 cut(s) | 12, 377, 494, 514, 1044 |
| DpnI | GATC | 6 cut(s) | 256, 267, 411, 767, 1021, 1251 |
| DpnII | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| Eam1104I | CTCTTC | 2 cut(s) | 167, 583 |
| EarI | CTCTTC | 2 cut(s) | 167, 583 |
| Ecl136II | GAGCTC | 1 cut(s) | 563 |
| Eco24I | GRGCYC | 1 cut(s) | 565 |
| Eco31I | GGTCTC | 1 cut(s) | 908 |
| Eco53kI | GAGCTC | 1 cut(s) | 563 |
| Eco57I | CTGAAG | 7 cut(s) | 581, 716, 760, 881, 969, 1161, 1233 |
| EcoICRI | GAGCTC | 1 cut(s) | 563 |
| EcoRI | GAATTC | 1 cut(s) | 525 |
| EcoRII | CCWGG | 1 cut(s) | 144 |
| EcoT38I | GRGCYC | 1 cut(s) | 565 |
| FaeI | CATG | 8 cut(s) | 51, 182, 304, 385, 493, 569, 772, 1009 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 810, 842 |
| FatI | CATG | 8 cut(s) | 47, 178, 300, 381, 489, 565, 768, 1005 |
| FbaI | TGATCA | 2 cut(s) | 265, 765 |
| Fnu4HI | GCNGC | 6 cut(s) | 23, 153, 644, 660, 857, 1032 |
| FokI | GGATG | 3 cut(s) | 755, 820, 924 |
| FriOI | GRGCYC | 1 cut(s) | 565 |
| Fsp4HI | GCNGC | 6 cut(s) | 23, 153, 644, 660, 857, 1032 |
| FspBI | CTAG | 2 cut(s) | 1167, 1220 |
| GlaI | GCGC | 1 cut(s) | 165 |
| GluI | GCNGC | 6 cut(s) | 23, 153, 644, 660, 857, 1032 |
| GsaI | CCCAGC | 1 cut(s) | 730 |
| GsuI | CTGGAG | 1 cut(s) | 659 |
| HaeIII | GGCC | 1 cut(s) | 1010 |
| HgaI | GACGC | 1 cut(s) | 1107 |
| HhaI | GCGC | 1 cut(s) | 166 |
| Hin1II | CATG | 8 cut(s) | 51, 182, 304, 385, 493, 569, 772, 1009 |
| Hin6I | GCGC | 1 cut(s) | 164 |
| HinP1I | GCGC | 1 cut(s) | 164 |
| HincII | GTYRAC | 1 cut(s) | 702 |
| HindII | GTYRAC | 1 cut(s) | 702 |
| HinfI | GANTC | 5 cut(s) | 575, 591, 746, 944, 1187 |
| HphI | GGTGA | 2 cut(s) | 439, 715 |
| Hpy166II | GTNNAC | 4 cut(s) | 623, 702, 723, 959 |
| Hpy188I | TCNGA | 8 cut(s) | 231, 259, 779, 1045, 1087, 1180, 1192, 1243 |
| Hpy188III | TCNNGA | 7 cut(s) | 301, 554, 614, 818, 827, 911, 941 |
| Hpy8I | GTNNAC | 4 cut(s) | 623, 702, 723, 959 |
| HpyAV | CCTTC | 5 cut(s) | 235, 458, 605, 644, 883 |
| HpyCH4III | ACNGT | 4 cut(s) | 671, 697, 883, 966 |
| HpyCH4IV | ACGT | 1 cut(s) | 1163 |
| HpyCH4V | TGCA | 9 cut(s) | 329, 365, 459, 623, 710, 836, 1058, 1094, 1106 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 22, 141, 161, 736, 862, 980, 1055, 1064 |
| HpyF3I | CTNAG | 5 cut(s) | 12, 377, 494, 514, 1044 |
| HpySE526I | ACGT | 1 cut(s) | 1163 |
| Hsp92II | CATG | 8 cut(s) | 51, 182, 304, 385, 493, 569, 772, 1009 |
| HspAI | GCGC | 1 cut(s) | 164 |
| Ksp22I | TGATCA | 2 cut(s) | 265, 765 |
| Kzo9I | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| LmnI | GCTCC | 3 cut(s) | 160, 640, 849 |
| Lsp1109I | GCAGC | 5 cut(s) | 9, 139, 630, 843, 1018 |
| LweI | GCATC | 4 cut(s) | 352, 970, 1018, 1222 |
| MaeI | CTAG | 2 cut(s) | 1167, 1220 |
| MaeII | ACGT | 1 cut(s) | 1163 |
| MaeIII | GTNAC | 1 cut(s) | 315 |
| MalI | GATC | 6 cut(s) | 256, 267, 411, 767, 1021, 1251 |
| MboI | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| MboII | GAAGA | 9 cut(s) | 126, 184, 215, 250, 570, 915, 1004, 1149, 1226 |
| MfeI | CAATTG | 3 cut(s) | 70, 198, 1095 |
| MhlI | GDGCHC | 4 cut(s) | 378, 565, 625, 802 |
| MlyI | GAGTC | 2 cut(s) | 569, 585 |
| MmeI | TCCRAC | 2 cut(s) | 182, 1129 |
| MnlI | CCTC | 8 cut(s) | 134, 379, 382, 385, 541, 865, 1039, 1145 |
| MseI | TTAA | 2 cut(s) | 423, 480 |
| MslI | CAYNNNNRTG | 2 cut(s) | 183, 324 |
| MspR9I | CCNGG | 1 cut(s) | 146 |
| MunI | CAATTG | 3 cut(s) | 70, 198, 1095 |
| MvaI | CCWGG | 1 cut(s) | 146 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 22, 141, 161, 736, 862, 980, 1055, 1064 |
| NdeII | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| NlaIII | CATG | 8 cut(s) | 51, 182, 304, 385, 493, 569, 772, 1009 |
| NspI | RCATGY | 1 cut(s) | 182 |
| NspV | TTCGAA | 1 cut(s) | 170 |
| PagI | TCATGA | 1 cut(s) | 300 |
| PfeI | GAWTC | 3 cut(s) | 746, 944, 1187 |
| PkrI | GCNGC | 6 cut(s) | 24, 154, 645, 661, 858, 1033 |
| PleI | GAGTC | 2 cut(s) | 569, 585 |
| PpsI | GAGTC | 2 cut(s) | 569, 585 |
| Psp124BI | GAGCTC | 1 cut(s) | 565 |
| Psp6I | CCWGG | 1 cut(s) | 144 |
| PspFI | CCCAGC | 1 cut(s) | 726 |
| PspGI | CCWGG | 1 cut(s) | 144 |
| PstI | CTGCAG | 1 cut(s) | 1060 |
| PstNI | CAGNNNCTG | 1 cut(s) | 739 |
| RsaI | GTAC | 5 cut(s) | 122, 219, 246, 968, 1002 |
| RsaNI | GTAC | 5 cut(s) | 121, 218, 245, 967, 1001 |
| RseI | CAYNNNNRTG | 2 cut(s) | 183, 324 |
| SacI | GAGCTC | 1 cut(s) | 565 |
| SaqAI | TTAA | 2 cut(s) | 423, 480 |
| SatI | GCNGC | 6 cut(s) | 23, 153, 644, 660, 857, 1032 |
| Sau3AI | GATC | 6 cut(s) | 254, 265, 409, 765, 1019, 1249 |
| ScaI | AGTACT | 1 cut(s) | 219 |
| SchI | GAGTC | 2 cut(s) | 569, 585 |
| ScrFI | CCNGG | 1 cut(s) | 146 |
| SduI | GDGCHC | 4 cut(s) | 378, 565, 625, 802 |
| SfaNI | GCATC | 4 cut(s) | 352, 970, 1018, 1222 |
| SfcI | CTRYAG | 2 cut(s) | 962, 1056 |
| SfuI | TTCGAA | 1 cut(s) | 170 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 183, 324 |
| SmlI | CTYRAG | 1 cut(s) | 533 |
| SmoI | CTYRAG | 1 cut(s) | 533 |
| SsiI | CCGC | 1 cut(s) | 659 |
| SspMI | CTAG | 2 cut(s) | 1167, 1220 |
| SstI | GAGCTC | 1 cut(s) | 565 |
| StyD4I | CCNGG | 1 cut(s) | 144 |
| TaaI | ACNGT | 4 cut(s) | 671, 697, 883, 966 |
| TaiI | ACGT | 1 cut(s) | 1166 |
| TaqI | TCGA | 3 cut(s) | 170, 464, 1252 |
| TatI | WGTACW | 1 cut(s) | 217 |
| TauI | GCSGC | 1 cut(s) | 662 |
| TfiI | GAWTC | 3 cut(s) | 746, 944, 1187 |
| Tru1I | TTAA | 2 cut(s) | 423, 480 |
| Tru9I | TTAA | 2 cut(s) | 423, 480 |
| TscAI | CASTG | 4 cut(s) | 522, 676, 888, 1128 |
| TseI | GCWGC | 5 cut(s) | 22, 152, 643, 856, 1031 |
| TspDTI | ATGAA | 4 cut(s) | 98, 180, 478, 759 |
| TspRI | CASTG | 4 cut(s) | 522, 676, 888, 1128 |
| VneI | GTGCAC | 1 cut(s) | 621 |
| XapI | RAATTY | 2 cut(s) | 525, 1108 |
| XceI | RCATGY | 1 cut(s) | 182 |
| XspI | CTAG | 2 cut(s) | 1167, 1220 |
| ZrmI | AGTACT | 1 cut(s) | 219 |