NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 159, 706 |
| AciI | CCGC | 4 cut(s) | 281, 338, 470, 1004 |
| AcsI | RAATTY | 3 cut(s) | 204, 802, 997 |
| AcuI | CTGAAG | 5 cut(s) | 395, 494, 582, 927, 969 |
| AfaI | GTAC | 2 cut(s) | 390, 983 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 280, 355, 412, 739 |
| AgsI | TTSAA | 7 cut(s) | 23, 35, 236, 457, 557, 605, 855 |
| AjnI | CCWGG | 1 cut(s) | 276 |
| AluBI | AGCT | 6 cut(s) | 242, 322, 409, 465, 608, 650 |
| AluI | AGCT | 6 cut(s) | 242, 322, 409, 465, 608, 650 |
| Alw21I | GWGCWC | 3 cut(s) | 135, 244, 304 |
| Alw26I | GTCTC | 3 cut(s) | 440, 521, 694 |
| Alw44I | GTGCAC | 1 cut(s) | 300 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 418, 725 |
| Ama87I | CYCGRG | 1 cut(s) | 697 |
| AoxI | GGCC | 1 cut(s) | 621 |
| ApaLI | GTGCAC | 1 cut(s) | 300 |
| ApeKI | GCWGC | 2 cut(s) | 322, 725 |
| ApoI | RAATTY | 3 cut(s) | 204, 802, 997 |
| AspS9I | GGNCC | 2 cut(s) | 673, 848 |
| AsuHPI | GGTGA | 3 cut(s) | 338, 394, 977 |
| AvaI | CYCGRG | 1 cut(s) | 697 |
| AvaII | GGWCC | 2 cut(s) | 673, 848 |
| BaeGI | GKGCMC | 1 cut(s) | 304 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 936, 969 |
| BanI | GGYRCC | 2 cut(s) | 159, 706 |
| BanII | GRGCYC | 1 cut(s) | 244 |
| BauI | CACGAG | 2 cut(s) | 261, 437 |
| Bbv12I | GWGCWC | 3 cut(s) | 135, 244, 304 |
| BbvI | GCAGC | 2 cut(s) | 309, 712 |
| BciT130I | CCWGG | 1 cut(s) | 278 |
| BcoDI | GTCTC | 3 cut(s) | 440, 521, 694 |
| BfaI | CTAG | 3 cut(s) | 861, 914, 957 |
| BfmI | CTRYAG | 2 cut(s) | 575, 750 |
| BisI | GCNGC | 4 cut(s) | 323, 339, 470, 726 |
| BlpI | GCTNAGC | 1 cut(s) | 134 |
| BlsI | GCNGC | 4 cut(s) | 324, 340, 471, 727 |
| BmcAI | AGTACT | 1 cut(s) | 983 |
| Bme1390I | CCNGG | 1 cut(s) | 278 |
| Bme18I | GGWCC | 2 cut(s) | 673, 848 |
| BmeT110I | CYCGRG | 1 cut(s) | 697 |
| BmgT120I | GGNCC | 2 cut(s) | 673, 848 |
| BmiI | GGNNCC | 2 cut(s) | 161, 708 |
| BmrFI | CCNGG | 1 cut(s) | 278 |
| BmsI | GCATC | 5 cut(s) | 109, 583, 712, 878, 916 |
| BpmI | CTGGAG | 2 cut(s) | 260, 338 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 134 |
| BpuEI | CTTGAG | 1 cut(s) | 233 |
| BsaI | GGTCTC | 1 cut(s) | 521 |
| BsaJI | CCNNGG | 1 cut(s) | 349 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 280, 355, 412, 739 |
| Bse1I | ACTGG | 4 cut(s) | 99, 161, 191, 321 |
| Bse3DI | GCAATG | 2 cut(s) | 129, 795 |
| BseBI | CCWGG | 1 cut(s) | 278 |
| BseDI | CCNNGG | 1 cut(s) | 349 |
| BseGI | GGATG | 1 cut(s) | 427 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 280, 355, 412, 739 |
| BseMI | GCAATG | 2 cut(s) | 129, 795 |
| BseMII | CTCAG | 3 cut(s) | 148, 207, 729 |
| BseNI | ACTGG | 4 cut(s) | 99, 161, 191, 321 |
| BseRI | GAGGAG | 2 cut(s) | 158, 263 |
| BseSI | GKGCMC | 1 cut(s) | 304 |
| BseXI | GCAGC | 2 cut(s) | 309, 712 |
| BseYI | CCCAGC | 1 cut(s) | 405 |
| BshFI | GGCC | 1 cut(s) | 623 |
| BshNI | GGYRCC | 2 cut(s) | 159, 706 |
| BsiHKAI | GWGCWC | 3 cut(s) | 135, 244, 304 |
| BsiHKCI | CYCGRG | 1 cut(s) | 697 |
| BslFI | GGGAC | 1 cut(s) | 679 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 280, 355, 412, 739 |
| BsmAI | GTCTC | 3 cut(s) | 440, 521, 694 |
| BsmFI | GGGAC | 1 cut(s) | 679 |
| BsnI | GGCC | 1 cut(s) | 623 |
| Bso31I | GGTCTC | 1 cut(s) | 521 |
| BsoBI | CYCGRG | 1 cut(s) | 697 |
| Bsp1286I | GDGCHC | 3 cut(s) | 135, 244, 304 |
| Bsp1407I | TGTACA | 1 cut(s) | 388 |
| Bsp143I | GATC | 1 cut(s) | 632 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 134 |
| BspACI | CCGC | 4 cut(s) | 281, 338, 470, 1004 |
| BspANI | GGCC | 1 cut(s) | 623 |
| BspCNI | CTCAG | 3 cut(s) | 147, 206, 730 |
| BspHI | TCATGA | 1 cut(s) | 57 |
| BspLI | GGNNCC | 2 cut(s) | 161, 708 |
| BspMAI | CTGCAG | 1 cut(s) | 754 |
| BspT107I | GGYRCC | 2 cut(s) | 159, 706 |
| BspTNI | GGTCTC | 1 cut(s) | 521 |
| BsrDI | GCAATG | 2 cut(s) | 129, 795 |
| BsrGI | TGTACA | 1 cut(s) | 388 |
| BsrI | ACTGG | 4 cut(s) | 99, 161, 191, 321 |
| BssECI | CCNNGG | 1 cut(s) | 349 |
| BssMI | GATC | 1 cut(s) | 632 |
| BssSI | CACGAG | 2 cut(s) | 261, 437 |
| Bst2BI | CACGAG | 2 cut(s) | 261, 437 |
| Bst2UI | CCWGG | 1 cut(s) | 278 |
| Bst4CI | ACNGT | 3 cut(s) | 350, 496, 579 |
| Bst6I | CTCTTC | 1 cut(s) | 262 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 593, 749, 758 |
| BstAUI | TGTACA | 1 cut(s) | 388 |
| BstC8I | GCNNGC | 2 cut(s) | 300, 467 |
| BstDEI | CTNAG | 4 cut(s) | 134, 193, 646, 738 |
| BstDSI | CCRYGG | 1 cut(s) | 349 |
| BstF5I | GGATG | 1 cut(s) | 427 |
| BstKTI | GATC | 1 cut(s) | 635 |
| BstMAI | GTCTC | 3 cut(s) | 440, 521, 694 |
| BstMBI | GATC | 1 cut(s) | 632 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 415, 475, 593, 647, 749, 758 |
| BstNI | CCWGG | 1 cut(s) | 278 |
| BstSCI | CCNGG | 1 cut(s) | 276 |
| BstSFI | CTRYAG | 2 cut(s) | 575, 750 |
| BstSLI | GKGCMC | 1 cut(s) | 304 |
| BstV1I | GCAGC | 2 cut(s) | 309, 712 |
| BsuRI | GGCC | 1 cut(s) | 623 |
| BtgI | CCRYGG | 1 cut(s) | 349 |
| BtsCI | GGATG | 1 cut(s) | 427 |
| BtsI | GCAGTG | 1 cut(s) | 689 |
| BtsIMutI | CAGTG | 5 cut(s) | 201, 501, 584, 689, 933 |
| Cac8I | GCNNGC | 2 cut(s) | 300, 467 |
| CaiI | CAGNNNCTG | 2 cut(s) | 418, 725 |
| CciI | TCATGA | 1 cut(s) | 57 |
| Cfr13I | GGNCC | 2 cut(s) | 673, 848 |
| CseI | GACGC | 1 cut(s) | 801 |
| Csp6I | GTAC | 2 cut(s) | 389, 982 |
| CviAII | CATG | 3 cut(s) | 58, 245, 619 |
| CviQI | GTAC | 2 cut(s) | 389, 982 |
| DdeI | CTNAG | 4 cut(s) | 134, 193, 646, 738 |
| DpnI | GATC | 1 cut(s) | 634 |
| DpnII | GATC | 1 cut(s) | 632 |
| Eam1104I | CTCTTC | 1 cut(s) | 262 |
| EarI | CTCTTC | 1 cut(s) | 262 |
| Ecl136II | GAGCTC | 1 cut(s) | 242 |
| Eco24I | GRGCYC | 1 cut(s) | 244 |
| Eco31I | GGTCTC | 1 cut(s) | 521 |
| Eco47I | GGWCC | 2 cut(s) | 673, 848 |
| Eco53kI | GAGCTC | 1 cut(s) | 242 |
| Eco57I | CTGAAG | 5 cut(s) | 395, 494, 582, 927, 969 |
| Eco88I | CYCGRG | 1 cut(s) | 697 |
| EcoICRI | GAGCTC | 1 cut(s) | 242 |
| EcoO109I | RGGNCCY | 1 cut(s) | 848 |
| EcoRI | GAATTC | 2 cut(s) | 204, 997 |
| EcoRII | CCWGG | 1 cut(s) | 276 |
| EcoT22I | ATGCAT | 2 cut(s) | 620, 871 |
| EcoT38I | GRGCYC | 1 cut(s) | 244 |
| FaeI | CATG | 3 cut(s) | 61, 248, 622 |
| FaqI | GGGAC | 1 cut(s) | 679 |
| FatI | CATG | 3 cut(s) | 57, 244, 618 |
| FauI | CCCGC | 2 cut(s) | 274, 997 |
| Fnu4HI | GCNGC | 4 cut(s) | 323, 339, 470, 726 |
| FokI | GGATG | 1 cut(s) | 434 |
| FriOI | GRGCYC | 1 cut(s) | 244 |
| Fsp4HI | GCNGC | 4 cut(s) | 323, 339, 470, 726 |
| FspBI | CTAG | 3 cut(s) | 861, 914, 957 |
| GluI | GCNGC | 4 cut(s) | 323, 339, 470, 726 |
| GsaI | CCCAGC | 1 cut(s) | 409 |
| GsuI | CTGGAG | 2 cut(s) | 260, 338 |
| HaeIII | GGCC | 1 cut(s) | 623 |
| HgaI | GACGC | 1 cut(s) | 801 |
| Hin1II | CATG | 3 cut(s) | 61, 248, 622 |
| HincII | GTYRAC | 1 cut(s) | 381 |
| HindII | GTYRAC | 1 cut(s) | 381 |
| HindIII | AAGCTT | 1 cut(s) | 606 |
| HinfI | GANTC | 5 cut(s) | 254, 270, 425, 557, 881 |
| HphI | GGTGA | 3 cut(s) | 338, 394, 977 |
| Hpy166II | GTNNAC | 5 cut(s) | 302, 381, 402, 572, 689 |
| Hpy188I | TCNGA | 4 cut(s) | 739, 781, 874, 886 |
| Hpy188III | TCNNGA | 8 cut(s) | 58, 233, 293, 439, 524, 554, 697, 699 |
| Hpy8I | GTNNAC | 5 cut(s) | 302, 381, 402, 572, 689 |
| HpyAV | CCTTC | 5 cut(s) | 221, 323, 367, 496, 861 |
| HpyCH4III | ACNGT | 3 cut(s) | 350, 496, 579 |
| HpyCH4IV | ACGT | 1 cut(s) | 857 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 415, 475, 593, 647, 749, 758 |
| HpyF3I | CTNAG | 4 cut(s) | 134, 193, 646, 738 |
| HpySE526I | ACGT | 1 cut(s) | 857 |
| Hsp92II | CATG | 3 cut(s) | 61, 248, 622 |
| Kzo9I | GATC | 1 cut(s) | 632 |
| LmnI | GCTCC | 2 cut(s) | 319, 462 |
| Lsp1109I | GCAGC | 2 cut(s) | 309, 712 |
| LweI | GCATC | 5 cut(s) | 109, 583, 712, 878, 916 |
| MaeI | CTAG | 3 cut(s) | 861, 914, 957 |
| MaeII | ACGT | 1 cut(s) | 857 |
| MaeIII | GTNAC | 3 cut(s) | 72, 152, 952 |
| MalI | GATC | 1 cut(s) | 634 |
| MboI | GATC | 1 cut(s) | 632 |
| MboII | GAAGA | 3 cut(s) | 249, 528, 920 |
| MfeI | CAATTG | 1 cut(s) | 789 |
| MhlI | GDGCHC | 3 cut(s) | 135, 244, 304 |
| MluCI | AATT | 8 cut(s) | 5, 109, 204, 532, 744, 789, 802, 997 |
| MlyI | GAGTC | 2 cut(s) | 248, 264 |
| MmeI | TCCRAC | 1 cut(s) | 823 |
| MnlI | CCTC | 8 cut(s) | 136, 139, 284, 478, 555, 656, 733, 839 |
| Mph1103I | ATGCAT | 2 cut(s) | 620, 871 |
| MseI | TTAA | 1 cut(s) | 99 |
| MslI | CAYNNNNRTG | 1 cut(s) | 81 |
| MspA1I | CMGCKG | 2 cut(s) | 472, 1004 |
| MspR9I | CCNGG | 1 cut(s) | 278 |
| MunI | CAATTG | 1 cut(s) | 789 |
| MvaI | CCWGG | 1 cut(s) | 278 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 415, 475, 593, 647, 749, 758 |
| NdeII | GATC | 1 cut(s) | 632 |
| NlaIII | CATG | 3 cut(s) | 61, 248, 622 |
| NlaIV | GGNNCC | 2 cut(s) | 161, 708 |
| NsiI | ATGCAT | 2 cut(s) | 620, 871 |
| PaeR7I | CTCGAG | 1 cut(s) | 697 |
| PagI | TCATGA | 1 cut(s) | 57 |
| PfeI | GAWTC | 3 cut(s) | 425, 557, 881 |
| PkrI | GCNGC | 4 cut(s) | 324, 340, 471, 727 |
| PleI | GAGTC | 2 cut(s) | 248, 264 |
| PpsI | GAGTC | 2 cut(s) | 248, 264 |
| PpuMI | RGGWCCY | 1 cut(s) | 848 |
| Psp124BI | GAGCTC | 1 cut(s) | 244 |
| Psp5II | RGGWCCY | 1 cut(s) | 848 |
| Psp6I | CCWGG | 1 cut(s) | 276 |
| PspFI | CCCAGC | 1 cut(s) | 405 |
| PspGI | CCWGG | 1 cut(s) | 276 |
| PspN4I | GGNNCC | 2 cut(s) | 161, 708 |
| PspPI | GGNCC | 2 cut(s) | 673, 848 |
| PspPPI | RGGWCCY | 1 cut(s) | 848 |
| PstI | CTGCAG | 1 cut(s) | 754 |
| PstNI | CAGNNNCTG | 2 cut(s) | 418, 725 |
| RsaI | GTAC | 2 cut(s) | 390, 983 |
| RsaNI | GTAC | 2 cut(s) | 389, 982 |
| RseI | CAYNNNNRTG | 1 cut(s) | 81 |
| SacI | GAGCTC | 1 cut(s) | 244 |
| SaqAI | TTAA | 1 cut(s) | 99 |
| SatI | GCNGC | 4 cut(s) | 323, 339, 470, 726 |
| Sau3AI | GATC | 1 cut(s) | 632 |
| Sau96I | GGNCC | 2 cut(s) | 673, 848 |
| ScaI | AGTACT | 1 cut(s) | 983 |
| SchI | GAGTC | 2 cut(s) | 248, 264 |
| ScrFI | CCNGG | 1 cut(s) | 278 |
| SduI | GDGCHC | 3 cut(s) | 135, 244, 304 |
| SfaNI | GCATC | 5 cut(s) | 109, 583, 712, 878, 916 |
| SfcI | CTRYAG | 2 cut(s) | 575, 750 |
| Sfr274I | CTCGAG | 1 cut(s) | 697 |
| SinI | GGWCC | 2 cut(s) | 673, 848 |
| SlaI | CTCGAG | 1 cut(s) | 697 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 81 |
| SmlI | CTYRAG | 2 cut(s) | 212, 697 |
| SmoI | CTYRAG | 2 cut(s) | 212, 697 |
| Sse9I | AATT | 8 cut(s) | 5, 109, 204, 532, 744, 789, 802, 997 |
| SsiI | CCGC | 4 cut(s) | 281, 338, 470, 1004 |
| SspMI | CTAG | 3 cut(s) | 861, 914, 957 |
| SstI | GAGCTC | 1 cut(s) | 244 |
| StyD4I | CCNGG | 1 cut(s) | 276 |
| TaaI | ACNGT | 3 cut(s) | 350, 496, 579 |
| TaiI | ACGT | 1 cut(s) | 860 |
| TaqI | TCGA | 2 cut(s) | 560, 698 |
| TasI | AATT | 8 cut(s) | 5, 109, 204, 532, 744, 789, 802, 997 |
| TatI | WGTACW | 2 cut(s) | 388, 981 |
| TauI | GCSGC | 2 cut(s) | 341, 472 |
| TfiI | GAWTC | 3 cut(s) | 425, 557, 881 |
| Tru1I | TTAA | 1 cut(s) | 99 |
| Tru9I | TTAA | 1 cut(s) | 99 |
| TscAI | CASTG | 5 cut(s) | 201, 501, 584, 696, 940 |
| TseI | GCWGC | 2 cut(s) | 322, 725 |
| TspDTI | ATGAA | 2 cut(s) | 386, 438 |
| TspRI | CASTG | 5 cut(s) | 201, 501, 584, 696, 940 |
| VneI | GTGCAC | 1 cut(s) | 300 |
| VpaK11BI | GGWCC | 2 cut(s) | 673, 848 |
| XapI | RAATTY | 3 cut(s) | 204, 802, 997 |
| XhoI | CTCGAG | 1 cut(s) | 697 |
| XspI | CTAG | 3 cut(s) | 861, 914, 957 |
| ZrmI | AGTACT | 1 cut(s) | 983 |
| Zsp2I | ATGCAT | 2 cut(s) | 620, 871 |