NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 205, 401 |
| AciI | CCGC | 1 cut(s) | 461 |
| AclWI | GGATC | 2 cut(s) | 325, 376 |
| AcuI | CTGAAG | 3 cut(s) | 518, 560, 753 |
| AfaI | GTAC | 4 cut(s) | 282, 309, 513, 814 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 478, 535 |
| AgsI | TTSAA | 7 cut(s) | 235, 266, 274, 287, 628, 646, 728 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 175 |
| AluBI | AGCT | 5 cut(s) | 161, 445, 532, 636, 779 |
| AluI | AGCT | 5 cut(s) | 161, 445, 532, 636, 779 |
| Alw21I | GWGCWC | 1 cut(s) | 352 |
| Alw26I | GTCTC | 3 cut(s) | 232, 317, 692 |
| AlwI | GGATC | 2 cut(s) | 325, 376 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 541 |
| AoxI | GGCC | 1 cut(s) | 792 |
| ApeKI | GCWGC | 2 cut(s) | 215, 445 |
| AspLEI | GCGC | 1 cut(s) | 229 |
| AsuHPI | GGTGA | 6 cut(s) | 16, 68, 130, 160, 461, 517 |
| BaeGI | GKGCMC | 1 cut(s) | 602 |
| BbsI | GAAGAC | 1 cut(s) | 171 |
| Bbv12I | GWGCWC | 1 cut(s) | 352 |
| BbvI | GCAGC | 2 cut(s) | 202, 432 |
| BccI | CCATC | 3 cut(s) | 133, 242, 393 |
| BceAI | ACGGC | 1 cut(s) | 625 |
| BclI | TGATCA | 1 cut(s) | 328 |
| BcoDI | GTCTC | 3 cut(s) | 232, 317, 692 |
| BfmI | CTRYAG | 2 cut(s) | 162, 746 |
| BisI | GCNGC | 3 cut(s) | 216, 446, 462 |
| BlpI | GCTNAGC | 1 cut(s) | 351 |
| BlsI | GCNGC | 3 cut(s) | 217, 447, 463 |
| BmcAI | AGTACT | 1 cut(s) | 282 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 175 |
| BmsI | GCATC | 2 cut(s) | 326, 754 |
| BpiI | GAAGAC | 1 cut(s) | 171 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 357, 389 |
| BpmI | CTGGAG | 1 cut(s) | 461 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 351 |
| BsaI | GGTCTC | 1 cut(s) | 692 |
| BsaJI | CCNNGG | 1 cut(s) | 472 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 20, 32, 50 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 478, 535 |
| Bse1I | ACTGG | 1 cut(s) | 444 |
| Bse3DI | GCAATG | 2 cut(s) | 254, 346 |
| BseDI | CCNNGG | 1 cut(s) | 472 |
| BseGI | GGATG | 2 cut(s) | 550, 613 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 478, 535 |
| BseMI | GCAATG | 2 cut(s) | 254, 346 |
| BseMII | CTCAG | 3 cut(s) | 223, 365, 594 |
| BseNI | ACTGG | 1 cut(s) | 444 |
| BseRI | GAGGAG | 1 cut(s) | 378 |
| BseSI | GKGCMC | 1 cut(s) | 602 |
| BseXI | GCAGC | 2 cut(s) | 202, 432 |
| BseYI | CCCAGC | 1 cut(s) | 528 |
| BshFI | GGCC | 1 cut(s) | 794 |
| BsiHKAI | GWGCWC | 1 cut(s) | 352 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 478, 535 |
| BsmAI | GTCTC | 3 cut(s) | 232, 317, 692 |
| BsnI | GGCC | 1 cut(s) | 794 |
| Bso31I | GGTCTC | 1 cut(s) | 692 |
| Bsp1286I | GDGCHC | 2 cut(s) | 352, 602 |
| Bsp1407I | TGTACA | 1 cut(s) | 511 |
| Bsp143I | GATC | 4 cut(s) | 317, 328, 381, 803 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 351 |
| BspACI | CCGC | 1 cut(s) | 461 |
| BspANI | GGCC | 1 cut(s) | 794 |
| BspCNI | CTCAG | 3 cut(s) | 222, 364, 595 |
| BspPI | GGATC | 2 cut(s) | 325, 376 |
| BspTNI | GGTCTC | 1 cut(s) | 692 |
| BsrDI | GCAATG | 2 cut(s) | 254, 346 |
| BsrGI | TGTACA | 1 cut(s) | 511 |
| BsrI | ACTGG | 1 cut(s) | 444 |
| BssECI | CCNNGG | 1 cut(s) | 472 |
| BssMI | GATC | 4 cut(s) | 317, 328, 381, 803 |
| Bst4CI | ACNGT | 4 cut(s) | 173, 473, 667, 750 |
| Bst6I | CTCTTC | 2 cut(s) | 230, 355 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 764 |
| BstAUI | TGTACA | 1 cut(s) | 511 |
| BstC8I | GCNNGC | 1 cut(s) | 638 |
| BstDEI | CTNAG | 3 cut(s) | 209, 351, 603 |
| BstDSI | CCRYGG | 1 cut(s) | 472 |
| BstF5I | GGATG | 2 cut(s) | 550, 613 |
| BstHHI | GCGC | 1 cut(s) | 229 |
| BstKTI | GATC | 4 cut(s) | 320, 331, 384, 806 |
| BstMAI | GTCTC | 3 cut(s) | 232, 317, 692 |
| BstMBI | GATC | 4 cut(s) | 317, 328, 381, 803 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 224, 538, 646, 764 |
| BstNSI | RCATGY | 1 cut(s) | 245 |
| BstSFI | CTRYAG | 2 cut(s) | 162, 746 |
| BstSLI | GKGCMC | 1 cut(s) | 602 |
| BstV1I | GCAGC | 2 cut(s) | 202, 432 |
| BstV2I | GAAGAC | 1 cut(s) | 171 |
| BsuRI | GGCC | 1 cut(s) | 794 |
| BtgI | CCRYGG | 1 cut(s) | 472 |
| BtgZI | GCGATG | 1 cut(s) | 349 |
| BtsCI | GGATG | 2 cut(s) | 550, 613 |
| BtsI | GCAGTG | 1 cut(s) | 96 |
| BtsIMutI | CAGTG | 4 cut(s) | 96, 600, 672, 755 |
| Cac8I | GCNNGC | 1 cut(s) | 638 |
| CaiI | CAGNNNCTG | 1 cut(s) | 541 |
| CfoI | GCGC | 1 cut(s) | 229 |
| Csp6I | GTAC | 4 cut(s) | 281, 308, 512, 813 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 34, 69 |
| CviAII | CATG | 3 cut(s) | 242, 356, 790 |
| CviJI | RGCY | 9 cut(s) | 161, 215, 369, 445, 532, 574, 636, 779, 794 |
| CviKI_1 | RGCY | 9 cut(s) | 161, 215, 369, 445, 532, 574, 636, 779, 794 |
| CviQI | GTAC | 4 cut(s) | 281, 308, 512, 813 |
| DdeI | CTNAG | 3 cut(s) | 209, 351, 603 |
| DpnI | GATC | 4 cut(s) | 319, 330, 383, 805 |
| DpnII | GATC | 4 cut(s) | 317, 328, 381, 803 |
| DriI | GACNNNNNGTC | 1 cut(s) | 175 |
| Eam1104I | CTCTTC | 2 cut(s) | 230, 355 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 175 |
| EarI | CTCTTC | 2 cut(s) | 230, 355 |
| Eco31I | GGTCTC | 1 cut(s) | 692 |
| Eco57I | CTGAAG | 3 cut(s) | 518, 560, 753 |
| EcoT22I | ATGCAT | 1 cut(s) | 791 |
| FaeI | CATG | 3 cut(s) | 245, 359, 793 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 388, 420 |
| FatI | CATG | 3 cut(s) | 241, 355, 789 |
| FauNDI | CATATG | 1 cut(s) | 73 |
| FbaI | TGATCA | 1 cut(s) | 328 |
| FblI | GTMKAC | 2 cut(s) | 205, 401 |
| Fnu4HI | GCNGC | 3 cut(s) | 216, 446, 462 |
| FokI | GGATG | 2 cut(s) | 557, 620 |
| Fsp4HI | GCNGC | 3 cut(s) | 216, 446, 462 |
| GlaI | GCGC | 1 cut(s) | 228 |
| GluI | GCNGC | 3 cut(s) | 216, 446, 462 |
| GsaI | CCCAGC | 1 cut(s) | 532 |
| GsuI | CTGGAG | 1 cut(s) | 461 |
| HaeIII | GGCC | 1 cut(s) | 794 |
| HhaI | GCGC | 1 cut(s) | 229 |
| Hin1II | CATG | 3 cut(s) | 245, 359, 793 |
| Hin6I | GCGC | 1 cut(s) | 227 |
| HinP1I | GCGC | 1 cut(s) | 227 |
| HincII | GTYRAC | 1 cut(s) | 504 |
| HindII | GTYRAC | 1 cut(s) | 504 |
| HindIII | AAGCTT | 1 cut(s) | 777 |
| HinfI | GANTC | 4 cut(s) | 207, 416, 548, 728 |
| HphI | GGTGA | 6 cut(s) | 16, 68, 130, 160, 461, 517 |
| Hpy166II | GTNNAC | 5 cut(s) | 206, 402, 504, 525, 743 |
| Hpy188I | TCNGA | 3 cut(s) | 294, 322, 579 |
| Hpy188III | TCNNGA | 3 cut(s) | 618, 695, 725 |
| Hpy8I | GTNNAC | 5 cut(s) | 206, 402, 504, 525, 743 |
| HpyAV | CCTTC | 6 cut(s) | 61, 79, 121, 446, 490, 667 |
| HpyCH4III | ACNGT | 4 cut(s) | 173, 473, 667, 750 |
| HpyCH4V | TGCA | 2 cut(s) | 339, 789 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 224, 538, 646, 764 |
| HpyF3I | CTNAG | 3 cut(s) | 209, 351, 603 |
| Hsp92II | CATG | 3 cut(s) | 245, 359, 793 |
| HspAI | GCGC | 1 cut(s) | 227 |
| Ksp22I | TGATCA | 1 cut(s) | 328 |
| Kzo9I | GATC | 4 cut(s) | 317, 328, 381, 803 |
| LmnI | GCTCC | 3 cut(s) | 223, 442, 633 |
| LpnPI | CCDG | 8 cut(s) | 196, 425, 434, 464, 521, 527, 542, 708 |
| Lsp1109I | GCAGC | 2 cut(s) | 202, 432 |
| LweI | GCATC | 2 cut(s) | 326, 754 |
| MalI | GATC | 4 cut(s) | 319, 330, 383, 805 |
| MboI | GATC | 4 cut(s) | 317, 328, 381, 803 |
| MboII | GAAGA | 7 cut(s) | 136, 171, 247, 278, 313, 372, 699 |
| MfeI | CAATTG | 1 cut(s) | 261 |
| MhlI | GDGCHC | 2 cut(s) | 352, 602 |
| MluCI | AATT | 2 cut(s) | 261, 703 |
| MlyI | GAGTC | 1 cut(s) | 201 |
| MmeI | TCCRAC | 2 cut(s) | 18, 245 |
| Mph1103I | ATGCAT | 1 cut(s) | 791 |
| MseI | TTAA | 2 cut(s) | 132, 395 |
| MslI | CAYNNNNRTG | 1 cut(s) | 246 |
| MunI | CAATTG | 1 cut(s) | 261 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 224, 538, 646, 764 |
| NdeI | CATATG | 1 cut(s) | 73 |
| NdeII | GATC | 4 cut(s) | 317, 328, 381, 803 |
| NlaIII | CATG | 3 cut(s) | 245, 359, 793 |
| NsiI | ATGCAT | 1 cut(s) | 791 |
| NspI | RCATGY | 1 cut(s) | 245 |
| PfeI | GAWTC | 3 cut(s) | 416, 548, 728 |
| PkrI | GCNGC | 3 cut(s) | 217, 447, 463 |
| PleI | GAGTC | 1 cut(s) | 201 |
| PpsI | GAGTC | 1 cut(s) | 201 |
| PspFI | CCCAGC | 1 cut(s) | 528 |
| PstNI | CAGNNNCTG | 1 cut(s) | 541 |
| RsaI | GTAC | 4 cut(s) | 282, 309, 513, 814 |
| RsaNI | GTAC | 4 cut(s) | 281, 308, 512, 813 |
| RseI | CAYNNNNRTG | 1 cut(s) | 246 |
| SaqAI | TTAA | 2 cut(s) | 132, 395 |
| SatI | GCNGC | 3 cut(s) | 216, 446, 462 |
| Sau3AI | GATC | 4 cut(s) | 317, 328, 381, 803 |
| ScaI | AGTACT | 1 cut(s) | 282 |
| SchI | GAGTC | 1 cut(s) | 201 |
| SduI | GDGCHC | 2 cut(s) | 352, 602 |
| SfaNI | GCATC | 2 cut(s) | 326, 754 |
| SfcI | CTRYAG | 2 cut(s) | 162, 746 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 246 |
| Sse9I | AATT | 2 cut(s) | 261, 703 |
| SsiI | CCGC | 1 cut(s) | 461 |
| TaaI | ACNGT | 4 cut(s) | 173, 473, 667, 750 |
| TaqI | TCGA | 2 cut(s) | 731, 774 |
| TasI | AATT | 2 cut(s) | 261, 703 |
| TatI | WGTACW | 3 cut(s) | 280, 307, 511 |
| TauI | GCSGC | 1 cut(s) | 464 |
| TfiI | GAWTC | 3 cut(s) | 416, 548, 728 |
| Tru1I | TTAA | 2 cut(s) | 132, 395 |
| Tru9I | TTAA | 2 cut(s) | 132, 395 |
| TscAI | CASTG | 4 cut(s) | 96, 607, 672, 755 |
| TseI | GCWGC | 2 cut(s) | 215, 445 |
| TspDTI | ATGAA | 3 cut(s) | 243, 509, 561 |
| TspGWI | ACGGA | 2 cut(s) | 175, 214 |
| TspRI | CASTG | 4 cut(s) | 96, 607, 672, 755 |
| XceI | RCATGY | 1 cut(s) | 245 |
| XmiI | GTMKAC | 2 cut(s) | 205, 401 |
| ZrmI | AGTACT | 1 cut(s) | 282 |
| Zsp2I | ATGCAT | 1 cut(s) | 791 |