NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1087 |
| AccI | GTMKAC | 2 cut(s) | 132, 843 |
| AciI | CCGC | 5 cut(s) | 304, 605, 662, 851, 1385 |
| AclWI | GGATC | 2 cut(s) | 295, 470 |
| AcsI | RAATTY | 3 cut(s) | 528, 1183, 1378 |
| AcuI | CTGAAG | 6 cut(s) | 584, 719, 763, 875, 963, 1308 |
| AfaI | GTAC | 5 cut(s) | 252, 279, 714, 996, 1364 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 604, 679, 736, 1120 |
| AgsI | TTSAA | 8 cut(s) | 236, 244, 257, 560, 938, 986, 1236, 1331 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 249, 281 |
| AluBI | AGCT | 6 cut(s) | 403, 566, 646, 733, 989, 1031 |
| AluI | AGCT | 6 cut(s) | 403, 566, 646, 733, 989, 1031 |
| Alw21I | GWGCWC | 3 cut(s) | 390, 568, 628 |
| Alw26I | GTCTC | 4 cut(s) | 202, 287, 414, 902 |
| Alw44I | GTGCAC | 1 cut(s) | 624 |
| AlwI | GGATC | 2 cut(s) | 295, 470 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 742, 1106 |
| Ama87I | CYCGRG | 1 cut(s) | 1078 |
| AoxI | GGCC | 1 cut(s) | 1002 |
| ApaLI | GTGCAC | 1 cut(s) | 624 |
| ApeKI | GCWGC | 4 cut(s) | 114, 185, 646, 1106 |
| ApoI | RAATTY | 3 cut(s) | 528, 1183, 1378 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 125, 157 |
| AspLEI | GCGC | 1 cut(s) | 199 |
| AspS9I | GGNCC | 2 cut(s) | 1054, 1229 |
| AsuHPI | GGTGA | 4 cut(s) | 505, 662, 718, 1358 |
| AvaI | CYCGRG | 1 cut(s) | 1078 |
| AvaII | GGWCC | 2 cut(s) | 1054, 1229 |
| BaeGI | GKGCMC | 2 cut(s) | 628, 805 |
| BanI | GGYRCC | 1 cut(s) | 1087 |
| BanII | GRGCYC | 1 cut(s) | 568 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 978, 1010 |
| BauI | CACGAG | 1 cut(s) | 585 |
| BbsI | GAAGAC | 1 cut(s) | 507 |
| Bbv12I | GWGCWC | 3 cut(s) | 390, 568, 628 |
| BbvI | GCAGC | 4 cut(s) | 101, 172, 633, 1093 |
| BccI | CCATC | 3 cut(s) | 212, 487, 839 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 14, 48 |
| BclI | TGATCA | 1 cut(s) | 298 |
| BcoDI | GTCTC | 4 cut(s) | 202, 287, 414, 902 |
| BfaI | CTAG | 5 cut(s) | 14, 71, 1242, 1295, 1338 |
| BfmI | CTRYAG | 1 cut(s) | 956 |
| BisI | GCNGC | 6 cut(s) | 115, 186, 647, 663, 851, 1107 |
| BlpI | GCTNAGC | 1 cut(s) | 389 |
| BlsI | GCNGC | 6 cut(s) | 116, 187, 648, 664, 852, 1108 |
| BmcAI | AGTACT | 2 cut(s) | 252, 1364 |
| Bme18I | GGWCC | 2 cut(s) | 1054, 1229 |
| BmeT110I | CYCGRG | 1 cut(s) | 1078 |
| BmgT120I | GGNCC | 2 cut(s) | 1054, 1229 |
| BmiI | GGNNCC | 1 cut(s) | 1089 |
| BmsI | GCATC | 6 cut(s) | 64, 364, 964, 1093, 1259, 1297 |
| BpiI | GAAGAC | 1 cut(s) | 507 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 451, 483 |
| BpmI | CTGGAG | 1 cut(s) | 662 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 389 |
| BpuEI | CTTGAG | 1 cut(s) | 557 |
| BsaI | GGTCTC | 1 cut(s) | 902 |
| BsaJI | CCNNGG | 1 cut(s) | 673 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 392, 422 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 604, 679, 736, 1120 |
| Bse1I | ACTGG | 3 cut(s) | 32, 515, 645 |
| Bse3DI | GCAATG | 3 cut(s) | 224, 384, 1176 |
| BseDI | CCNNGG | 1 cut(s) | 673 |
| BseGI | GGATG | 2 cut(s) | 751, 816 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 604, 679, 736, 1120 |
| BseMI | GCAATG | 3 cut(s) | 224, 384, 1176 |
| BseMII | CTCAG | 5 cut(s) | 150, 403, 531, 797, 1110 |
| BseNI | ACTGG | 3 cut(s) | 32, 515, 645 |
| BseRI | GAGGAG | 1 cut(s) | 413 |
| BseSI | GKGCMC | 2 cut(s) | 628, 805 |
| BseXI | GCAGC | 4 cut(s) | 101, 172, 633, 1093 |
| BseYI | CCCAGC | 1 cut(s) | 729 |
| BshFI | GGCC | 1 cut(s) | 1004 |
| BshNI | GGYRCC | 1 cut(s) | 1087 |
| BsiHKAI | GWGCWC | 3 cut(s) | 390, 568, 628 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1078 |
| BsiSI | CCGG | 1 cut(s) | 118 |
| BslFI | GGGAC | 2 cut(s) | 1060, 1095 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 604, 679, 736, 1120 |
| BsmAI | GTCTC | 4 cut(s) | 202, 287, 414, 902 |
| BsmFI | GGGAC | 2 cut(s) | 1060, 1095 |
| BsnI | GGCC | 1 cut(s) | 1004 |
| Bso31I | GGTCTC | 1 cut(s) | 902 |
| BsoBI | CYCGRG | 1 cut(s) | 1078 |
| Bsp1286I | GDGCHC | 4 cut(s) | 390, 568, 628, 805 |
| Bsp1407I | TGTACA | 1 cut(s) | 712 |
| Bsp143I | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 389 |
| BspACI | CCGC | 5 cut(s) | 304, 605, 662, 851, 1385 |
| BspANI | GGCC | 1 cut(s) | 1004 |
| BspCNI | CTCAG | 5 cut(s) | 149, 402, 530, 798, 1111 |
| BspHI | TCATGA | 1 cut(s) | 312 |
| BspLI | GGNNCC | 1 cut(s) | 1089 |
| BspPI | GGATC | 2 cut(s) | 295, 470 |
| BspT107I | GGYRCC | 1 cut(s) | 1087 |
| BspTNI | GGTCTC | 1 cut(s) | 902 |
| BsrDI | GCAATG | 3 cut(s) | 224, 384, 1176 |
| BsrGI | TGTACA | 1 cut(s) | 712 |
| BsrI | ACTGG | 3 cut(s) | 32, 515, 645 |
| BssECI | CCNNGG | 1 cut(s) | 673 |
| BssMI | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| BssNAI | GTATAC | 1 cut(s) | 844 |
| BssSI | CACGAG | 1 cut(s) | 585 |
| Bst1107I | GTATAC | 1 cut(s) | 844 |
| Bst2BI | CACGAG | 1 cut(s) | 585 |
| Bst4CI | ACNGT | 3 cut(s) | 674, 877, 960 |
| Bst6I | CTCTTC | 2 cut(s) | 200, 586 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 974, 1130 |
| BstAUI | TGTACA | 1 cut(s) | 712 |
| BstC8I | GCNNGC | 2 cut(s) | 37, 624 |
| BstDEI | CTNAG | 6 cut(s) | 136, 389, 517, 806, 1027, 1119 |
| BstDSI | CCRYGG | 1 cut(s) | 673 |
| BstF5I | GGATG | 2 cut(s) | 751, 816 |
| BstHHI | GCGC | 1 cut(s) | 199 |
| BstKTI | GATC | 4 cut(s) | 290, 301, 478, 1016 |
| BstMAI | GTCTC | 4 cut(s) | 202, 287, 414, 902 |
| BstMBI | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 194, 739, 856, 974, 1028, 1130 |
| BstNSI | RCATGY | 1 cut(s) | 215 |
| BstSFI | CTRYAG | 1 cut(s) | 956 |
| BstSLI | GKGCMC | 2 cut(s) | 628, 805 |
| BstV1I | GCAGC | 4 cut(s) | 101, 172, 633, 1093 |
| BstV2I | GAAGAC | 1 cut(s) | 507 |
| BstZ17I | GTATAC | 1 cut(s) | 844 |
| BsuRI | GGCC | 1 cut(s) | 1004 |
| BtgI | CCRYGG | 1 cut(s) | 673 |
| BtsCI | GGATG | 2 cut(s) | 751, 816 |
| BtsI | GCAGTG | 1 cut(s) | 1070 |
| BtsIMutI | CAGTG | 6 cut(s) | 525, 803, 882, 965, 1070, 1314 |
| Cac8I | GCNNGC | 2 cut(s) | 37, 624 |
| CaiI | CAGNNNCTG | 2 cut(s) | 742, 1106 |
| CciI | TCATGA | 1 cut(s) | 312 |
| CfoI | GCGC | 1 cut(s) | 199 |
| Cfr13I | GGNCC | 2 cut(s) | 1054, 1229 |
| CseI | GACGC | 1 cut(s) | 1182 |
| Csp6I | GTAC | 5 cut(s) | 251, 278, 713, 995, 1363 |
| CviAII | CATG | 6 cut(s) | 40, 212, 313, 394, 569, 1000 |
| CviQI | GTAC | 5 cut(s) | 251, 278, 713, 995, 1363 |
| DdeI | CTNAG | 6 cut(s) | 136, 389, 517, 806, 1027, 1119 |
| DpnI | GATC | 4 cut(s) | 289, 300, 477, 1015 |
| DpnII | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| Eam1104I | CTCTTC | 2 cut(s) | 200, 586 |
| EarI | CTCTTC | 2 cut(s) | 200, 586 |
| Ecl136II | GAGCTC | 1 cut(s) | 566 |
| Eco24I | GRGCYC | 1 cut(s) | 568 |
| Eco31I | GGTCTC | 1 cut(s) | 902 |
| Eco47I | GGWCC | 2 cut(s) | 1054, 1229 |
| Eco53kI | GAGCTC | 1 cut(s) | 566 |
| Eco57I | CTGAAG | 6 cut(s) | 584, 719, 763, 875, 963, 1308 |
| Eco88I | CYCGRG | 1 cut(s) | 1078 |
| EcoICRI | GAGCTC | 1 cut(s) | 566 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1229 |
| EcoRI | GAATTC | 2 cut(s) | 528, 1378 |
| EcoT22I | ATGCAT | 1 cut(s) | 1252 |
| EcoT38I | GRGCYC | 1 cut(s) | 568 |
| FaeI | CATG | 6 cut(s) | 43, 215, 316, 397, 572, 1003 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 540, 572 |
| FaqI | GGGAC | 2 cut(s) | 1060, 1095 |
| FatI | CATG | 6 cut(s) | 39, 211, 312, 393, 568, 999 |
| FauI | CCCGC | 3 cut(s) | 297, 598, 1378 |
| FbaI | TGATCA | 1 cut(s) | 298 |
| FblI | GTMKAC | 2 cut(s) | 132, 843 |
| Fnu4HI | GCNGC | 6 cut(s) | 115, 186, 647, 663, 851, 1107 |
| FokI | GGATG | 2 cut(s) | 758, 823 |
| FriOI | GRGCYC | 1 cut(s) | 568 |
| Fsp4HI | GCNGC | 6 cut(s) | 115, 186, 647, 663, 851, 1107 |
| FspBI | CTAG | 5 cut(s) | 14, 71, 1242, 1295, 1338 |
| GlaI | GCGC | 1 cut(s) | 198 |
| GluI | GCNGC | 6 cut(s) | 115, 186, 647, 663, 851, 1107 |
| GsaI | CCCAGC | 1 cut(s) | 733 |
| GsuI | CTGGAG | 1 cut(s) | 662 |
| HaeIII | GGCC | 1 cut(s) | 1004 |
| HapII | CCGG | 1 cut(s) | 118 |
| HgaI | GACGC | 1 cut(s) | 1182 |
| HhaI | GCGC | 1 cut(s) | 199 |
| Hin1II | CATG | 6 cut(s) | 43, 215, 316, 397, 572, 1003 |
| Hin6I | GCGC | 1 cut(s) | 197 |
| HinP1I | GCGC | 1 cut(s) | 197 |
| HincII | GTYRAC | 1 cut(s) | 705 |
| HindII | GTYRAC | 1 cut(s) | 705 |
| HindIII | AAGCTT | 1 cut(s) | 987 |
| HinfI | GANTC | 6 cut(s) | 43, 134, 578, 749, 938, 1262 |
| HpaII | CCGG | 1 cut(s) | 118 |
| HphI | GGTGA | 4 cut(s) | 505, 662, 718, 1358 |
| Hpy166II | GTNNAC | 7 cut(s) | 133, 626, 705, 726, 844, 953, 1070 |
| Hpy188I | TCNGA | 8 cut(s) | 162, 264, 292, 782, 1120, 1162, 1255, 1267 |
| Hpy188III | TCNNGA | 9 cut(s) | 14, 71, 313, 557, 617, 821, 905, 935, 1080 |
| Hpy8I | GTNNAC | 7 cut(s) | 133, 626, 705, 726, 844, 953, 1070 |
| HpyAV | CCTTC | 8 cut(s) | 115, 119, 545, 608, 647, 691, 877, 1242 |
| HpyCH4III | ACNGT | 3 cut(s) | 674, 877, 960 |
| HpyCH4IV | ACGT | 1 cut(s) | 1238 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 194, 739, 856, 974, 1028, 1130 |
| HpyF3I | CTNAG | 6 cut(s) | 136, 389, 517, 806, 1027, 1119 |
| HpySE526I | ACGT | 1 cut(s) | 1238 |
| Hsp92II | CATG | 6 cut(s) | 43, 215, 316, 397, 572, 1003 |
| HspAI | GCGC | 1 cut(s) | 197 |
| Ksp22I | TGATCA | 1 cut(s) | 298 |
| Kzo9I | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| LmnI | GCTCC | 4 cut(s) | 163, 193, 400, 643 |
| Lsp1109I | GCAGC | 4 cut(s) | 101, 172, 633, 1093 |
| LweI | GCATC | 6 cut(s) | 64, 364, 964, 1093, 1259, 1297 |
| MaeI | CTAG | 5 cut(s) | 14, 71, 1242, 1295, 1338 |
| MaeII | ACGT | 1 cut(s) | 1238 |
| MaeIII | GTNAC | 3 cut(s) | 145, 327, 1333 |
| MalI | GATC | 4 cut(s) | 289, 300, 477, 1015 |
| MboI | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| MboII | GAAGA | 9 cut(s) | 29, 86, 217, 248, 283, 428, 512, 573, 1301 |
| MfeI | CAATTG | 2 cut(s) | 231, 1170 |
| MhlI | GDGCHC | 4 cut(s) | 390, 568, 628, 805 |
| MluCI | AATT | 7 cut(s) | 231, 364, 528, 1125, 1170, 1183, 1378 |
| MlyI | GAGTC | 3 cut(s) | 37, 128, 572 |
| MmeI | TCCRAC | 2 cut(s) | 215, 1204 |
| MnlI | CCTC | 9 cut(s) | 74, 391, 453, 801, 859, 936, 1037, 1114, 1220 |
| Mph1103I | ATGCAT | 1 cut(s) | 1252 |
| MseI | TTAA | 4 cut(s) | 104, 354, 489, 591 |
| MslI | CAYNNNNRTG | 2 cut(s) | 216, 336 |
| MspA1I | CMGCKG | 3 cut(s) | 304, 853, 1385 |
| MspI | CCGG | 1 cut(s) | 118 |
| MunI | CAATTG | 2 cut(s) | 231, 1170 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 194, 739, 856, 974, 1028, 1130 |
| NdeII | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| NlaIII | CATG | 6 cut(s) | 43, 215, 316, 397, 572, 1003 |
| NlaIV | GGNNCC | 1 cut(s) | 1089 |
| NmuCI | GTSAC | 1 cut(s) | 145 |
| NsiI | ATGCAT | 1 cut(s) | 1252 |
| NspI | RCATGY | 1 cut(s) | 215 |
| PagI | TCATGA | 1 cut(s) | 312 |
| PfeI | GAWTC | 3 cut(s) | 749, 938, 1262 |
| PkrI | GCNGC | 6 cut(s) | 116, 187, 648, 664, 852, 1108 |
| PleI | GAGTC | 3 cut(s) | 37, 128, 572 |
| PpsI | GAGTC | 3 cut(s) | 37, 128, 572 |
| PpuMI | RGGWCCY | 1 cut(s) | 1229 |
| Psp124BI | GAGCTC | 1 cut(s) | 568 |
| Psp5II | RGGWCCY | 1 cut(s) | 1229 |
| PspFI | CCCAGC | 1 cut(s) | 729 |
| PspN4I | GGNNCC | 1 cut(s) | 1089 |
| PspPI | GGNCC | 2 cut(s) | 1054, 1229 |
| PspPPI | RGGWCCY | 1 cut(s) | 1229 |
| PstNI | CAGNNNCTG | 2 cut(s) | 742, 1106 |
| RsaI | GTAC | 5 cut(s) | 252, 279, 714, 996, 1364 |
| RsaNI | GTAC | 5 cut(s) | 251, 278, 713, 995, 1363 |
| RseI | CAYNNNNRTG | 2 cut(s) | 216, 336 |
| SacI | GAGCTC | 1 cut(s) | 568 |
| SaqAI | TTAA | 4 cut(s) | 104, 354, 489, 591 |
| SatI | GCNGC | 6 cut(s) | 115, 186, 647, 663, 851, 1107 |
| Sau3AI | GATC | 4 cut(s) | 287, 298, 475, 1013 |
| Sau96I | GGNCC | 2 cut(s) | 1054, 1229 |
| ScaI | AGTACT | 2 cut(s) | 252, 1364 |
| SchI | GAGTC | 3 cut(s) | 37, 128, 572 |
| SduI | GDGCHC | 4 cut(s) | 390, 568, 628, 805 |
| SfaNI | GCATC | 6 cut(s) | 64, 364, 964, 1093, 1259, 1297 |
| SfcI | CTRYAG | 1 cut(s) | 956 |
| SinI | GGWCC | 2 cut(s) | 1054, 1229 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 216, 336 |
| SmlI | CTYRAG | 1 cut(s) | 536 |
| SmoI | CTYRAG | 1 cut(s) | 536 |
| Sse9I | AATT | 7 cut(s) | 231, 364, 528, 1125, 1170, 1183, 1378 |
| SsiI | CCGC | 5 cut(s) | 304, 605, 662, 851, 1385 |
| SspMI | CTAG | 5 cut(s) | 14, 71, 1242, 1295, 1338 |
| SstI | GAGCTC | 1 cut(s) | 568 |
| TaaI | ACNGT | 3 cut(s) | 674, 877, 960 |
| TaiI | ACGT | 1 cut(s) | 1241 |
| TaqI | TCGA | 2 cut(s) | 203, 941 |
| TasI | AATT | 7 cut(s) | 231, 364, 528, 1125, 1170, 1183, 1378 |
| TatI | WGTACW | 4 cut(s) | 250, 277, 712, 1362 |
| TauI | GCSGC | 2 cut(s) | 665, 853 |
| TfiI | GAWTC | 3 cut(s) | 749, 938, 1262 |
| Tru1I | TTAA | 4 cut(s) | 104, 354, 489, 591 |
| Tru9I | TTAA | 4 cut(s) | 104, 354, 489, 591 |
| TscAI | CASTG | 6 cut(s) | 525, 810, 882, 965, 1077, 1321 |
| TseFI | GTSAC | 1 cut(s) | 145 |
| TseI | GCWGC | 4 cut(s) | 114, 185, 646, 1106 |
| Tsp45I | GTSAC | 1 cut(s) | 145 |
| TspDTI | ATGAA | 4 cut(s) | 213, 437, 710, 762 |
| TspRI | CASTG | 6 cut(s) | 525, 810, 882, 965, 1077, 1321 |
| VneI | GTGCAC | 1 cut(s) | 624 |
| VpaK11BI | GGWCC | 2 cut(s) | 1054, 1229 |
| XapI | RAATTY | 3 cut(s) | 528, 1183, 1378 |
| XbaI | TCTAGA | 2 cut(s) | 13, 70 |
| XceI | RCATGY | 1 cut(s) | 215 |
| XmiI | GTMKAC | 2 cut(s) | 132, 843 |
| XspI | CTAG | 5 cut(s) | 14, 71, 1242, 1295, 1338 |
| ZrmI | AGTACT | 2 cut(s) | 252, 1364 |
| Zsp2I | ATGCAT | 1 cut(s) | 1252 |