NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 284, 512 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 424 |
| AciI | CCGC | 1 cut(s) | 350 |
| AclWI | GGATC | 1 cut(s) | 107 |
| AcsI | RAATTY | 1 cut(s) | 216 |
| AcuI | CTGAAG | 4 cut(s) | 272, 445, 566, 654 |
| AfaI | GTAC | 2 cut(s) | 687, 715 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 424 |
| AgsI | TTSAA | 3 cut(s) | 248, 529, 677 |
| AjnI | CCWGG | 1 cut(s) | 417 |
| AleI | CACNNNNGTG | 1 cut(s) | 419 |
| AluBI | AGCT | 5 cut(s) | 130, 254, 334, 537, 680 |
| AluI | AGCT | 5 cut(s) | 130, 254, 334, 537, 680 |
| Alw21I | GWGCWC | 3 cut(s) | 81, 256, 316 |
| Alw26I | GTCTC | 2 cut(s) | 183, 593 |
| Alw44I | GTGCAC | 1 cut(s) | 312 |
| AlwI | GGATC | 1 cut(s) | 107 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 424 |
| AoxI | GGCC | 1 cut(s) | 693 |
| ApaLI | GTGCAC | 1 cut(s) | 312 |
| ApeKI | GCWGC | 1 cut(s) | 334 |
| ApoI | RAATTY | 1 cut(s) | 216 |
| AsuHPI | GGTGA | 1 cut(s) | 406 |
| BaeGI | GKGCMC | 2 cut(s) | 316, 487 |
| BanII | GRGCYC | 1 cut(s) | 256 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 669, 701 |
| BauI | CACGAG | 1 cut(s) | 273 |
| Bbv12I | GWGCWC | 3 cut(s) | 81, 256, 316 |
| BbvI | GCAGC | 1 cut(s) | 321 |
| BccI | CCATC | 1 cut(s) | 124 |
| BciT130I | CCWGG | 1 cut(s) | 419 |
| BclI | TGATCA | 1 cut(s) | 450 |
| BcoDI | GTCTC | 2 cut(s) | 183, 593 |
| BfmI | CTRYAG | 1 cut(s) | 647 |
| BfuAI | ACCTGC | 2 cut(s) | 284, 512 |
| BisI | GCNGC | 2 cut(s) | 335, 351 |
| BlpI | GCTNAGC | 1 cut(s) | 80 |
| BlsI | GCNGC | 2 cut(s) | 336, 352 |
| Bme1390I | CCNGG | 1 cut(s) | 419 |
| BmrFI | CCNGG | 1 cut(s) | 419 |
| BmrI | ACTGGG | 1 cut(s) | 497 |
| BmsI | GCATC | 2 cut(s) | 55, 655 |
| BmuI | ACTGGG | 1 cut(s) | 497 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 86, 118 |
| BpmI | CTGGAG | 1 cut(s) | 350 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 80 |
| BpuEI | CTTGAG | 1 cut(s) | 245 |
| Bsa29I | ATCGAT | 1 cut(s) | 159 |
| BsaI | GGTCTC | 1 cut(s) | 593 |
| BsaJI | CCNNGG | 1 cut(s) | 417 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 424 |
| Bse1I | ACTGG | 4 cut(s) | 203, 333, 492, 659 |
| Bse3DI | GCAATG | 1 cut(s) | 75 |
| BseBI | CCWGG | 1 cut(s) | 419 |
| BseCI | ATCGAT | 1 cut(s) | 159 |
| BseDI | CCNNGG | 1 cut(s) | 417 |
| BseGI | GGATG | 2 cut(s) | 433, 498 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 424 |
| BseMI | GCAATG | 1 cut(s) | 75 |
| BseMII | CTCAG | 2 cut(s) | 94, 219 |
| BseNI | ACTGG | 4 cut(s) | 203, 333, 492, 659 |
| BseRI | GAGGAG | 2 cut(s) | 104, 107 |
| BseSI | GKGCMC | 2 cut(s) | 316, 487 |
| BseXI | GCAGC | 1 cut(s) | 321 |
| BshFI | GGCC | 1 cut(s) | 695 |
| BshVI | ATCGAT | 1 cut(s) | 159 |
| BsiHKAI | GWGCWC | 3 cut(s) | 81, 256, 316 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 424 |
| BsmAI | GTCTC | 2 cut(s) | 183, 593 |
| BsnI | GGCC | 1 cut(s) | 695 |
| Bso31I | GGTCTC | 1 cut(s) | 593 |
| Bsp1286I | GDGCHC | 4 cut(s) | 81, 256, 316, 487 |
| Bsp143I | GATC | 3 cut(s) | 112, 450, 704 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 80 |
| BspACI | CCGC | 1 cut(s) | 350 |
| BspANI | GGCC | 1 cut(s) | 695 |
| BspCNI | CTCAG | 2 cut(s) | 93, 218 |
| BspDI | ATCGAT | 1 cut(s) | 159 |
| BspMI | ACCTGC | 2 cut(s) | 284, 512 |
| BspPI | GGATC | 1 cut(s) | 107 |
| BspTNI | GGTCTC | 1 cut(s) | 593 |
| BsrDI | GCAATG | 1 cut(s) | 75 |
| BsrI | ACTGG | 4 cut(s) | 203, 333, 492, 659 |
| BssECI | CCNNGG | 1 cut(s) | 417 |
| BssMI | GATC | 3 cut(s) | 112, 450, 704 |
| BssSI | CACGAG | 1 cut(s) | 273 |
| Bst2BI | CACGAG | 1 cut(s) | 273 |
| Bst2UI | CCWGG | 1 cut(s) | 419 |
| Bst4CI | ACNGT | 4 cut(s) | 362, 388, 568, 651 |
| Bst6I | CTCTTC | 1 cut(s) | 274 |
| BstC8I | GCNNGC | 3 cut(s) | 312, 539, 656 |
| BstDEI | CTNAG | 3 cut(s) | 80, 185, 205 |
| BstF5I | GGATG | 2 cut(s) | 433, 498 |
| BstKTI | GATC | 3 cut(s) | 115, 453, 707 |
| BstMAI | GTCTC | 2 cut(s) | 183, 593 |
| BstMBI | GATC | 3 cut(s) | 112, 450, 704 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 547 |
| BstNI | CCWGG | 1 cut(s) | 419 |
| BstSCI | CCNGG | 1 cut(s) | 417 |
| BstSFI | CTRYAG | 1 cut(s) | 647 |
| BstSLI | GKGCMC | 2 cut(s) | 316, 487 |
| BstV1I | GCAGC | 1 cut(s) | 321 |
| Bsu15I | ATCGAT | 1 cut(s) | 159 |
| BsuRI | GGCC | 1 cut(s) | 695 |
| BsuTUI | ATCGAT | 1 cut(s) | 159 |
| BtsCI | GGATG | 2 cut(s) | 433, 498 |
| BtsIMutI | CAGTG | 5 cut(s) | 213, 367, 485, 573, 656 |
| BveI | ACCTGC | 2 cut(s) | 284, 512 |
| Cac8I | GCNNGC | 3 cut(s) | 312, 539, 656 |
| CaiI | CAGNNNCTG | 1 cut(s) | 424 |
| ClaI | ATCGAT | 1 cut(s) | 159 |
| Csp6I | GTAC | 2 cut(s) | 686, 714 |
| CviAII | CATG | 5 cut(s) | 85, 181, 257, 454, 691 |
| CviQI | GTAC | 2 cut(s) | 686, 714 |
| DdeI | CTNAG | 3 cut(s) | 80, 185, 205 |
| DpnI | GATC | 3 cut(s) | 114, 452, 706 |
| DpnII | GATC | 3 cut(s) | 112, 450, 704 |
| Eam1104I | CTCTTC | 1 cut(s) | 274 |
| EarI | CTCTTC | 1 cut(s) | 274 |
| Ecl136II | GAGCTC | 1 cut(s) | 254 |
| Eco24I | GRGCYC | 1 cut(s) | 256 |
| Eco31I | GGTCTC | 1 cut(s) | 593 |
| Eco53kI | GAGCTC | 1 cut(s) | 254 |
| Eco57I | CTGAAG | 4 cut(s) | 272, 445, 566, 654 |
| EcoICRI | GAGCTC | 1 cut(s) | 254 |
| EcoRI | GAATTC | 1 cut(s) | 216 |
| EcoRII | CCWGG | 1 cut(s) | 417 |
| EcoT38I | GRGCYC | 1 cut(s) | 256 |
| FaeI | CATG | 5 cut(s) | 88, 184, 260, 457, 694 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 495, 527 |
| FatI | CATG | 5 cut(s) | 84, 180, 256, 453, 690 |
| FbaI | TGATCA | 1 cut(s) | 450 |
| Fnu4HI | GCNGC | 2 cut(s) | 335, 351 |
| FokI | GGATG | 2 cut(s) | 440, 505 |
| FriOI | GRGCYC | 1 cut(s) | 256 |
| Fsp4HI | GCNGC | 2 cut(s) | 335, 351 |
| GluI | GCNGC | 2 cut(s) | 335, 351 |
| GsuI | CTGGAG | 1 cut(s) | 350 |
| HaeIII | GGCC | 1 cut(s) | 695 |
| Hin1II | CATG | 5 cut(s) | 88, 184, 260, 457, 694 |
| HincII | GTYRAC | 1 cut(s) | 393 |
| HindII | GTYRAC | 1 cut(s) | 393 |
| HindIII | AAGCTT | 1 cut(s) | 678 |
| HinfI | GANTC | 4 cut(s) | 266, 282, 431, 629 |
| HphI | GGTGA | 1 cut(s) | 406 |
| Hpy166II | GTNNAC | 4 cut(s) | 314, 393, 414, 644 |
| Hpy188I | TCNGA | 2 cut(s) | 400, 464 |
| Hpy188III | TCNNGA | 5 cut(s) | 245, 305, 503, 512, 596 |
| Hpy8I | GTNNAC | 4 cut(s) | 314, 393, 414, 644 |
| HpyAV | CCTTC | 4 cut(s) | 153, 296, 335, 568 |
| HpyCH4III | ACNGT | 4 cut(s) | 362, 388, 568, 651 |
| HpyCH4V | TGCA | 5 cut(s) | 29, 68, 154, 314, 521 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 547 |
| HpyF3I | CTNAG | 3 cut(s) | 80, 185, 205 |
| Hsp92II | CATG | 5 cut(s) | 88, 184, 260, 457, 694 |
| Ksp22I | TGATCA | 1 cut(s) | 450 |
| Kzo9I | GATC | 3 cut(s) | 112, 450, 704 |
| LmnI | GCTCC | 2 cut(s) | 331, 534 |
| Lsp1109I | GCAGC | 1 cut(s) | 321 |
| LweI | GCATC | 2 cut(s) | 55, 655 |
| MaeIII | GTNAC | 1 cut(s) | 15 |
| MalI | GATC | 3 cut(s) | 114, 452, 706 |
| MboI | GATC | 3 cut(s) | 112, 450, 704 |
| MboII | GAAGA | 1 cut(s) | 261 |
| MfeI | CAATTG | 1 cut(s) | 604 |
| MhlI | GDGCHC | 4 cut(s) | 81, 256, 316, 487 |
| MluCI | AATT | 3 cut(s) | 52, 216, 604 |
| MlyI | GAGTC | 2 cut(s) | 260, 276 |
| MnlI | CCTC | 5 cut(s) | 82, 85, 88, 232, 550 |
| MseI | TTAA | 2 cut(s) | 42, 126 |
| MslI | CAYNNNNRTG | 2 cut(s) | 24, 419 |
| MspR9I | CCNGG | 1 cut(s) | 419 |
| MunI | CAATTG | 1 cut(s) | 604 |
| MvaI | CCWGG | 1 cut(s) | 419 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 547 |
| NdeII | GATC | 3 cut(s) | 112, 450, 704 |
| NlaIII | CATG | 5 cut(s) | 88, 184, 260, 457, 694 |
| OliI | CACNNNNGTG | 1 cut(s) | 419 |
| PfeI | GAWTC | 2 cut(s) | 431, 629 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 424 |
| PkrI | GCNGC | 2 cut(s) | 336, 352 |
| PleI | GAGTC | 2 cut(s) | 260, 276 |
| PpsI | GAGTC | 2 cut(s) | 260, 276 |
| Psp124BI | GAGCTC | 1 cut(s) | 256 |
| Psp6I | CCWGG | 1 cut(s) | 417 |
| PspGI | CCWGG | 1 cut(s) | 417 |
| PstNI | CAGNNNCTG | 1 cut(s) | 424 |
| RsaI | GTAC | 2 cut(s) | 687, 715 |
| RsaNI | GTAC | 2 cut(s) | 686, 714 |
| RseI | CAYNNNNRTG | 2 cut(s) | 24, 419 |
| SacI | GAGCTC | 1 cut(s) | 256 |
| SaqAI | TTAA | 2 cut(s) | 42, 126 |
| SatI | GCNGC | 2 cut(s) | 335, 351 |
| Sau3AI | GATC | 3 cut(s) | 112, 450, 704 |
| SchI | GAGTC | 2 cut(s) | 260, 276 |
| ScrFI | CCNGG | 1 cut(s) | 419 |
| SduI | GDGCHC | 4 cut(s) | 81, 256, 316, 487 |
| SfaNI | GCATC | 2 cut(s) | 55, 655 |
| SfcI | CTRYAG | 1 cut(s) | 647 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 24, 419 |
| SmlI | CTYRAG | 1 cut(s) | 224 |
| SmoI | CTYRAG | 1 cut(s) | 224 |
| Sse9I | AATT | 3 cut(s) | 52, 216, 604 |
| SsiI | CCGC | 1 cut(s) | 350 |
| SstI | GAGCTC | 1 cut(s) | 256 |
| StyD4I | CCNGG | 1 cut(s) | 417 |
| TaaI | ACNGT | 4 cut(s) | 362, 388, 568, 651 |
| TaqI | TCGA | 2 cut(s) | 135, 159 |
| TasI | AATT | 3 cut(s) | 52, 216, 604 |
| TauI | GCSGC | 1 cut(s) | 353 |
| TfiI | GAWTC | 2 cut(s) | 431, 629 |
| Tru1I | TTAA | 2 cut(s) | 42, 126 |
| Tru9I | TTAA | 2 cut(s) | 42, 126 |
| TscAI | CASTG | 5 cut(s) | 213, 367, 492, 573, 656 |
| TseI | GCWGC | 1 cut(s) | 334 |
| TspDTI | ATGAA | 2 cut(s) | 169, 444 |
| TspRI | CASTG | 5 cut(s) | 213, 367, 492, 573, 656 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 424 |
| VneI | GTGCAC | 1 cut(s) | 312 |
| XapI | RAATTY | 1 cut(s) | 216 |