Rh4AG250800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000694
Physical Location & Seq
Forward (+)
56305237 .. 56332781
27545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG250800.1

Sequence Viewer

Length: 588 bp
ATGTCCAAGATCGCCGCGATCCGCTTCTCCAGCGAAGGAGCTGTCCGCCGTGGTCCAGAAGGATCCGGTGGTGGCTCAGGTCGGCGGTCCGAGCAAGCCACCGGAAAAGTCAGGCGGTTTGGAAGGACGAGGCATCTGAAATCTGTTTGTCGGATAAACAACGACGAAATTCAACACCGCAGCAATCAGCTAGATACTTTAACCAGTCACTTGCCAGATAACTCTCTACCAGACTATATATACAACTTCATCAAGGGTCCAACTGACTCCCGGCCCTGCTGTCCGACTTTAACCAGTCACTTGCCAGATAACTCTCTACCAGACTATATATACAACTTCATCAAGGGTCCAACTGACTCCCGGCCCTGCTGTCCGTCAACAGCGCCACTTCTATCTCGCCGTCGACAGACTCCAGTTCAAGATGTTGTTTGTGTCCCTCAGTCCATCGAGTCCCTCACTCTATTTCCCACTATAAATCAGACGCACATCCCCTACAATGGGGACTCCACCATTTCAATCAAGACCCCTTTCCCTCTGCTCTGGTGTCTTGTGAACCTAACTAAACGACACCCTCGCCATGTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

195

Amino Acids

21.79

Weight (kDa)

9.59

Isoelectric Point (pI)

64.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000694)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr1g0314531 RchiOBHm_Chr1g0314541 RchiOBHm_Chr1g0314551 RchiOBHm_Chr1g0341141 RchiOBHm_Chr1g0341151 RchiOBHm_Chr2g0147321 RchiOBHm_Chr2g0147341 RchiOBHm_Chr5g0016131 RchiOBHm_Chr5g0016141 RchiOBHm_Chr5g0016151 RchiOBHm_Chr5g0016251 RchiOBHm_Chr5g0029761 RchiOBHm_Chr5g0061311 RchiOBHm_Chr5g0069491 RchiOBHm_Chr5g0072041 RchiOBHm_Chr5g0072051 RchiOBHm_Chr5g0079831 RchiOBHm_Chr7g0226011 RchiOBHm_Chr7g0241351 RchiOBHm_Chr7g0241361 RchiOBHm_Chr7g0241381
rosa_laevigata RLG00000012856 RLG00000014927 RLG00000036057 RLG00000036066 RLG00000036073
rosa_multiflora Rmu_co8366475.1_g000001 Rmu_sc0000281.1_g000023 Rmu_sc0000689.1_g000025 Rmu_sc0000689.1_g000026 Rmu_sc0000848.1_g000013 Rmu_sc0001264.1_g000010 Rmu_sc0002132.1_g000040 Rmu_sc0003241.1_g000001 Rmu_sc0007385.1_g000008 Rmu_sc0007385.1_g000015 Rmu_sc0010665.1_g000021 Rmu_sc0015027.1_g000003 Rmu_sc0021542.1_g000001 Rmu_sc0023463.1_g000002 Rmu_sc0031947.1_g000001
rosa_roxburghii Rroxscaffold_1G00011470 Rroxscaffold_1G00057460 Rroxscaffold_2G00135270 Rroxscaffold_3G00255510 Rroxscaffold_5G00365380
rosa_rugosa Rorug07G0218300
rosa_samantha Rh1AG011200 Rh1AG011300 Rh2BG272300 Rh2BG272400 Rh2BG536800 Rh3DG285000 Rh3DG285100 Rh4AG250800 Rh4BG145100 Rh5BG183100 Rh5BG474300 Rh5CG135400 Rh5CG135800 Rh5CG135900 Rh5CG496700 Rh5CG497800 Rh6AG068300 Rh6AG140800 Rh7BG191500 Rh7CG198900 Rh7CG436200 Rh7CG436300
rosa_wichuraiana Rw1G023280 Rw2G052210 Rw6G005990 Rw6G021050 Rw7G010560 Rw7G042590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 403
AccII CGCG 1 cut(s) 17
AciI CCGC 6 cut(s) 15, 22, 46, 85, 115, 178
AclWI GGATC 3 cut(s) 13, 57, 70
AcsI RAATTY 1 cut(s) 168
AfiI CCNNNNNNNGG 2 cut(s) 497, 498
AgsI TTSAA 3 cut(s) 173, 419, 516
AluBI AGCT 2 cut(s) 41, 190
AluI AGCT 2 cut(s) 41, 190
AlwI GGATC 3 cut(s) 13, 57, 70
AoxI GGCC 2 cut(s) 272, 362
ApeKI GCWGC 1 cut(s) 180
ApoI RAATTY 1 cut(s) 168
ArsI GACNNNNNNTTYG 2 cut(s) 27, 59
AspLEI GCGC 1 cut(s) 385
AspS9I GGNCC 6 cut(s) 53, 87, 257, 273, 347, 363
AsuC2I CCSGG 2 cut(s) 271, 361
AvaII GGWCC 4 cut(s) 53, 87, 257, 347
BamHI GGATCC 1 cut(s) 62
BbvI GCAGC 1 cut(s) 192
BccI CCATC 1 cut(s) 452
BceAI ACGGC 2 cut(s) 33, 384
BcnI CCSGG 2 cut(s) 271, 361
BfaI CTAG 1 cut(s) 191
BfoI RGCGCY 1 cut(s) 386
BisI GCNGC 2 cut(s) 15, 181
BlsI GCNGC 2 cut(s) 16, 182
Bme1390I CCNGG 2 cut(s) 271, 361
Bme18I GGWCC 4 cut(s) 53, 87, 257, 347
BmgT120I GGNCC 6 cut(s) 53, 87, 257, 273, 347, 363
BmiI GGNNCC 3 cut(s) 64, 258, 348
BmrFI CCNGG 2 cut(s) 271, 361
BmsI GCATC 1 cut(s) 142
BpmI CTGGAG 2 cut(s) 13, 396
Bpu10I CCTNAGC 1 cut(s) 76
BpuMI CCSGG 2 cut(s) 271, 361
BsaJI CCNNGG 1 cut(s) 49
BsaWI WCCGGW 2 cut(s) 65, 101
Bsc4I CCNNNNNNNGG 2 cut(s) 497, 498
Bse1I ACTGG 3 cut(s) 204, 294, 413
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 486
BseLI CCNNNNNNNGG 2 cut(s) 497, 498
BseMII CTCAG 2 cut(s) 90, 452
BseNI ACTGG 3 cut(s) 204, 294, 413
BseXI GCAGC 1 cut(s) 192
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 274, 364
BsiSI CCGG 4 cut(s) 66, 102, 271, 361
BslFI GGGAC 3 cut(s) 419, 436, 515
BslI CCNNNNNNNGG 2 cut(s) 497, 498
BsmFI GGGAC 3 cut(s) 419, 436, 515
BsnI GGCC 2 cut(s) 274, 364
Bsp143I GATC 3 cut(s) 9, 18, 62
BspACI CCGC 6 cut(s) 15, 22, 46, 85, 115, 178
BspANI GGCC 2 cut(s) 274, 364
BspCNI CTCAG 2 cut(s) 89, 451
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 3 cut(s) 64, 258, 348
BspPI GGATC 3 cut(s) 13, 57, 70
BsrI ACTGG 3 cut(s) 204, 294, 413
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 3 cut(s) 9, 18, 62
BstC8I GCNNGC 1 cut(s) 96
BstDEI CTNAG 2 cut(s) 76, 438
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 486
BstFNI CGCG 1 cut(s) 17
BstH2I RGCGCY 1 cut(s) 386
BstHHI GCGC 1 cut(s) 385
BstKTI GATC 3 cut(s) 12, 21, 65
BstMBI GATC 3 cut(s) 9, 18, 62
BstMWI GCNNNNNNNGC 2 cut(s) 30, 91
BstSCI CCNGG 2 cut(s) 269, 359
BstUI CGCG 1 cut(s) 17
BstV1I GCAGC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 2 cut(s) 274, 364
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 486
Cac8I GCNNGC 1 cut(s) 96
CfoI GCGC 1 cut(s) 385
Cfr13I GGNCC 6 cut(s) 53, 87, 257, 273, 347, 363
CpoI CGGWCCG 1 cut(s) 87
CseI GACGC 1 cut(s) 490
CspI CGGWCCG 1 cut(s) 87
CviAII CATG 1 cut(s) 578
CviJI RGCY 6 cut(s) 41, 75, 98, 190, 274, 364
CviKI_1 RGCY 6 cut(s) 41, 75, 98, 190, 274, 364
DdeI CTNAG 2 cut(s) 76, 438
DpnI GATC 3 cut(s) 11, 20, 64
DpnII GATC 3 cut(s) 9, 18, 62
EciI GGCGGA 1 cut(s) 35
Eco47I GGWCC 4 cut(s) 53, 87, 257, 347
FaeI CATG 1 cut(s) 581
FaiI YATR 8 cut(s) 237, 239, 241, 327, 329, 331, 473, 579
FaqI GGGAC 3 cut(s) 419, 436, 515
FatI CATG 1 cut(s) 577
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 2 cut(s) 15, 181
FokI GGATG 1 cut(s) 473
Fsp4HI GCNGC 2 cut(s) 15, 181
FspBI CTAG 1 cut(s) 191
GlaI GCGC 1 cut(s) 384
GluI GCNGC 2 cut(s) 15, 181
GsuI CTGGAG 2 cut(s) 13, 396
HaeII RGCGCY 1 cut(s) 386
HaeIII GGCC 2 cut(s) 274, 364
HapII CCGG 4 cut(s) 66, 102, 271, 361
HgaI GACGC 1 cut(s) 490
HhaI GCGC 1 cut(s) 385
Hin1II CATG 1 cut(s) 581
Hin6I GCGC 1 cut(s) 383
HinP1I GCGC 1 cut(s) 383
HincII GTYRAC 2 cut(s) 378, 404
HindII GTYRAC 2 cut(s) 378, 404
HinfI GANTC 5 cut(s) 266, 356, 409, 449, 503
HpaII CCGG 4 cut(s) 66, 102, 271, 361
Hpy166II GTNNAC 3 cut(s) 378, 404, 553
Hpy188I TCNGA 5 cut(s) 91, 138, 153, 285, 480
Hpy188III TCNNGA 3 cut(s) 56, 419, 520
Hpy8I GTNNAC 3 cut(s) 378, 404, 553
Hpy99I CGWCG 2 cut(s) 167, 405
HpyAV CCTTC 3 cut(s) 29, 53, 117
HpyF10VI GCNNNNNNNGC 2 cut(s) 30, 91
HpyF3I CTNAG 2 cut(s) 76, 438
Hsp92II CATG 1 cut(s) 581
HspAI GCGC 1 cut(s) 383
Kzo9I GATC 3 cut(s) 9, 18, 62
LmnI GCTCC 1 cut(s) 38
Lsp1109I GCAGC 1 cut(s) 192
LweI GCATC 1 cut(s) 142
MaeI CTAG 1 cut(s) 191
MaeIII GTNAC 2 cut(s) 206, 296
MalI GATC 3 cut(s) 11, 20, 64
MboI GATC 3 cut(s) 9, 18, 62
MflI RGATCY 1 cut(s) 62
MluCI AATT 2 cut(s) 168, 583
MlyI GAGTC 5 cut(s) 260, 350, 403, 458, 497
MmeI TCCRAC 4 cut(s) 131, 284, 308, 374
MnlI CCTC 5 cut(s) 123, 447, 464, 543, 582
MseI TTAA 3 cut(s) 200, 290, 586
MspI CCGG 4 cut(s) 66, 102, 271, 361
MspR9I CCNGG 2 cut(s) 271, 361
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 2 cut(s) 30, 91
NciI CCSGG 2 cut(s) 271, 361
NdeII GATC 3 cut(s) 9, 18, 62
NlaIII CATG 1 cut(s) 581
NlaIV GGNNCC 3 cut(s) 64, 258, 348
NmuCI GTSAC 2 cut(s) 206, 296
PkrI GCNGC 2 cut(s) 16, 182
PleI GAGTC 5 cut(s) 260, 350, 403, 457, 497
PpsI GAGTC 5 cut(s) 260, 350, 403, 457, 497
PspN4I GGNNCC 3 cut(s) 64, 258, 348
PspPI GGNCC 6 cut(s) 53, 87, 257, 273, 347, 363
PsuI RGATCY 1 cut(s) 62
Rsr2I CGGWCCG 1 cut(s) 87
RsrII CGGWCCG 1 cut(s) 87
SalI GTCGAC 1 cut(s) 402
SaqAI TTAA 3 cut(s) 200, 290, 586
SatI GCNGC 2 cut(s) 15, 181
Sau3AI GATC 3 cut(s) 9, 18, 62
Sau96I GGNCC 6 cut(s) 53, 87, 257, 273, 347, 363
SchI GAGTC 5 cut(s) 260, 350, 403, 458, 497
ScrFI CCNGG 2 cut(s) 271, 361
SetI ASST 4 cut(s) 43, 82, 192, 558
SfaNI GCATC 1 cut(s) 142
SinI GGWCC 4 cut(s) 53, 87, 257, 347
Sse9I AATT 2 cut(s) 168, 583
SsiI CCGC 6 cut(s) 15, 22, 46, 85, 115, 178
SspMI CTAG 1 cut(s) 191
StyD4I CCNGG 2 cut(s) 269, 359
TaqI TCGA 2 cut(s) 403, 447
TasI AATT 2 cut(s) 168, 583
TauI GCSGC 1 cut(s) 17
Tru1I TTAA 3 cut(s) 200, 290, 586
Tru9I TTAA 3 cut(s) 200, 290, 586
TseFI GTSAC 2 cut(s) 206, 296
TseI GCWGC 1 cut(s) 180
Tsp45I GTSAC 2 cut(s) 206, 296
TspDTI ATGAA 2 cut(s) 238, 328
TspGWI ACGGA 1 cut(s) 363
VpaK11BI GGWCC 4 cut(s) 53, 87, 257, 347
XapI RAATTY 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 403
XspI CTAG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.