NEDD8 ultimate buster
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 3 cut(s) | 214, 476, 533 |
| AclWI | GGATC | 1 cut(s) | 205 |
| AcsI | RAATTY | 1 cut(s) | 399 |
| AfaI | GTAC | 3 cut(s) | 162, 189, 585 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 56, 475, 550 |
| AgsI | TTSAA | 5 cut(s) | 17, 146, 154, 167, 431 |
| AjnI | CCWGG | 2 cut(s) | 471, 600 |
| AluBI | AGCT | 3 cut(s) | 313, 437, 517 |
| AluI | AGCT | 3 cut(s) | 313, 437, 517 |
| Alw21I | GWGCWC | 3 cut(s) | 300, 439, 499 |
| Alw26I | GTCTC | 3 cut(s) | 112, 197, 366 |
| Alw44I | GTGCAC | 1 cut(s) | 495 |
| AlwI | GGATC | 1 cut(s) | 205 |
| AoxI | GGCC | 1 cut(s) | 622 |
| ApaLI | GTGCAC | 1 cut(s) | 495 |
| ApeKI | GCWGC | 2 cut(s) | 95, 517 |
| ApoI | RAATTY | 1 cut(s) | 399 |
| AspLEI | GCGC | 1 cut(s) | 109 |
| AspS9I | GGNCC | 1 cut(s) | 622 |
| AsuHPI | GGTGA | 2 cut(s) | 533, 589 |
| BaeGI | GKGCMC | 1 cut(s) | 499 |
| BanII | GRGCYC | 1 cut(s) | 439 |
| BauI | CACGAG | 1 cut(s) | 456 |
| Bbv12I | GWGCWC | 3 cut(s) | 300, 439, 499 |
| BbvI | GCAGC | 2 cut(s) | 82, 504 |
| BccI | CCATC | 1 cut(s) | 122 |
| BciT130I | CCWGG | 2 cut(s) | 473, 602 |
| BclI | TGATCA | 1 cut(s) | 208 |
| BcoDI | GTCTC | 3 cut(s) | 112, 197, 366 |
| BisI | GCNGC | 3 cut(s) | 96, 518, 534 |
| BlpI | GCTNAGC | 1 cut(s) | 299 |
| BlsI | GCNGC | 3 cut(s) | 97, 519, 535 |
| BmcAI | AGTACT | 1 cut(s) | 162 |
| Bme1390I | CCNGG | 2 cut(s) | 473, 602 |
| BmgT120I | GGNCC | 1 cut(s) | 622 |
| BmrFI | CCNGG | 2 cut(s) | 473, 602 |
| BmsI | GCATC | 1 cut(s) | 274 |
| BpmI | CTGGAG | 2 cut(s) | 455, 533 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 299 |
| BpuEI | CTTGAG | 1 cut(s) | 428 |
| Bsa29I | ATCGAT | 1 cut(s) | 342 |
| BsaJI | CCNNGG | 2 cut(s) | 544, 600 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 56, 475, 550 |
| Bse1I | ACTGG | 2 cut(s) | 386, 516 |
| Bse3DI | GCAATG | 2 cut(s) | 134, 294 |
| BseBI | CCWGG | 2 cut(s) | 473, 602 |
| BseCI | ATCGAT | 1 cut(s) | 342 |
| BseDI | CCNNGG | 2 cut(s) | 544, 600 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 56, 475, 550 |
| BseMI | GCAATG | 2 cut(s) | 134, 294 |
| BseMII | CTCAG | 2 cut(s) | 313, 402 |
| BseNI | ACTGG | 2 cut(s) | 386, 516 |
| BseRI | GAGGAG | 2 cut(s) | 323, 458 |
| BseSI | GKGCMC | 1 cut(s) | 499 |
| BseXI | GCAGC | 2 cut(s) | 82, 504 |
| BshFI | GGCC | 1 cut(s) | 624 |
| BshVI | ATCGAT | 1 cut(s) | 342 |
| BsiHKAI | GWGCWC | 3 cut(s) | 300, 439, 499 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 56, 475, 550 |
| BsmAI | GTCTC | 3 cut(s) | 112, 197, 366 |
| BsnI | GGCC | 1 cut(s) | 624 |
| Bsp1286I | GDGCHC | 3 cut(s) | 300, 439, 499 |
| Bsp1407I | TGTACA | 1 cut(s) | 583 |
| Bsp143I | GATC | 2 cut(s) | 197, 208 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 299 |
| BspACI | CCGC | 3 cut(s) | 214, 476, 533 |
| BspANI | GGCC | 1 cut(s) | 624 |
| BspCNI | CTCAG | 2 cut(s) | 312, 401 |
| BspDI | ATCGAT | 1 cut(s) | 342 |
| BspHI | TCATGA | 1 cut(s) | 222 |
| BspPI | GGATC | 1 cut(s) | 205 |
| BsrDI | GCAATG | 2 cut(s) | 134, 294 |
| BsrGI | TGTACA | 1 cut(s) | 583 |
| BsrI | ACTGG | 2 cut(s) | 386, 516 |
| BssECI | CCNNGG | 2 cut(s) | 544, 600 |
| BssMI | GATC | 2 cut(s) | 197, 208 |
| BssSI | CACGAG | 1 cut(s) | 456 |
| Bst2BI | CACGAG | 1 cut(s) | 456 |
| Bst2UI | CCWGG | 2 cut(s) | 473, 602 |
| Bst4CI | ACNGT | 2 cut(s) | 10, 545 |
| Bst6I | CTCTTC | 2 cut(s) | 110, 457 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 71 |
| BstAUI | TGTACA | 1 cut(s) | 583 |
| BstC8I | GCNNGC | 2 cut(s) | 216, 495 |
| BstDEI | CTNAG | 3 cut(s) | 299, 368, 388 |
| BstDSI | CCRYGG | 1 cut(s) | 544 |
| BstHHI | GCGC | 1 cut(s) | 109 |
| BstKTI | GATC | 2 cut(s) | 200, 211 |
| BstMAI | GTCTC | 3 cut(s) | 112, 197, 366 |
| BstMBI | GATC | 2 cut(s) | 197, 208 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 71, 104 |
| BstNI | CCWGG | 2 cut(s) | 473, 602 |
| BstNSI | RCATGY | 1 cut(s) | 125 |
| BstSCI | CCNGG | 2 cut(s) | 471, 600 |
| BstSLI | GKGCMC | 1 cut(s) | 499 |
| BstV1I | GCAGC | 2 cut(s) | 82, 504 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 608 |
| Bsu15I | ATCGAT | 1 cut(s) | 342 |
| BsuRI | GGCC | 1 cut(s) | 624 |
| BsuTUI | ATCGAT | 1 cut(s) | 342 |
| BtgI | CCRYGG | 1 cut(s) | 544 |
| BtsIMutI | CAGTG | 2 cut(s) | 396, 634 |
| Cac8I | GCNNGC | 2 cut(s) | 216, 495 |
| CciI | TCATGA | 1 cut(s) | 222 |
| CfoI | GCGC | 1 cut(s) | 109 |
| Cfr13I | GGNCC | 1 cut(s) | 622 |
| ClaI | ATCGAT | 1 cut(s) | 342 |
| Csp6I | GTAC | 3 cut(s) | 161, 188, 584 |
| CviAII | CATG | 9 cut(s) | 42, 52, 122, 223, 304, 364, 440, 626, 631 |
| CviJI | RGCY | 8 cut(s) | 95, 231, 313, 385, 416, 437, 517, 624 |
| CviKI_1 | RGCY | 8 cut(s) | 95, 231, 313, 385, 416, 437, 517, 624 |
| CviQI | GTAC | 3 cut(s) | 161, 188, 584 |
| DdeI | CTNAG | 3 cut(s) | 299, 368, 388 |
| DpnI | GATC | 2 cut(s) | 199, 210 |
| DpnII | GATC | 2 cut(s) | 197, 208 |
| Eam1104I | CTCTTC | 2 cut(s) | 110, 457 |
| EarI | CTCTTC | 2 cut(s) | 110, 457 |
| Ecl136II | GAGCTC | 1 cut(s) | 437 |
| Eco24I | GRGCYC | 1 cut(s) | 439 |
| Eco53kI | GAGCTC | 1 cut(s) | 437 |
| EcoICRI | GAGCTC | 1 cut(s) | 437 |
| EcoRI | GAATTC | 1 cut(s) | 399 |
| EcoRII | CCWGG | 2 cut(s) | 471, 600 |
| EcoT38I | GRGCYC | 1 cut(s) | 439 |
| FaeI | CATG | 9 cut(s) | 45, 55, 125, 226, 307, 367, 443, 629, 634 |
| FatI | CATG | 9 cut(s) | 41, 51, 121, 222, 303, 363, 439, 625, 630 |
| FauI | CCCGC | 2 cut(s) | 207, 469 |
| FbaI | TGATCA | 1 cut(s) | 208 |
| Fnu4HI | GCNGC | 3 cut(s) | 96, 518, 534 |
| FriOI | GRGCYC | 1 cut(s) | 439 |
| Fsp4HI | GCNGC | 3 cut(s) | 96, 518, 534 |
| GlaI | GCGC | 1 cut(s) | 108 |
| GluI | GCNGC | 3 cut(s) | 96, 518, 534 |
| GsuI | CTGGAG | 2 cut(s) | 455, 533 |
| HaeIII | GGCC | 1 cut(s) | 624 |
| HhaI | GCGC | 1 cut(s) | 109 |
| Hin1II | CATG | 9 cut(s) | 45, 55, 125, 226, 307, 367, 443, 629, 634 |
| Hin6I | GCGC | 1 cut(s) | 107 |
| HinP1I | GCGC | 1 cut(s) | 107 |
| HincII | GTYRAC | 1 cut(s) | 576 |
| HindII | GTYRAC | 1 cut(s) | 576 |
| HinfI | GANTC | 3 cut(s) | 21, 449, 465 |
| HphI | GGTGA | 2 cut(s) | 533, 589 |
| Hpy166II | GTNNAC | 3 cut(s) | 497, 576, 597 |
| Hpy188I | TCNGA | 3 cut(s) | 26, 174, 202 |
| Hpy188III | TCNNGA | 4 cut(s) | 14, 223, 428, 488 |
| Hpy8I | GTNNAC | 3 cut(s) | 497, 576, 597 |
| HpyAV | CCTTC | 5 cut(s) | 336, 416, 518, 562, 613 |
| HpyCH4III | ACNGT | 2 cut(s) | 10, 545 |
| HpyCH4V | TGCA | 6 cut(s) | 41, 251, 287, 337, 497, 634 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 71, 104 |
| HpyF3I | CTNAG | 3 cut(s) | 299, 368, 388 |
| Hsp92II | CATG | 9 cut(s) | 45, 55, 125, 226, 307, 367, 443, 629, 634 |
| HspAI | GCGC | 1 cut(s) | 107 |
| Ksp22I | TGATCA | 1 cut(s) | 208 |
| Kzo9I | GATC | 2 cut(s) | 197, 208 |
| LmnI | GCTCC | 3 cut(s) | 103, 310, 514 |
| Lsp1109I | GCAGC | 2 cut(s) | 82, 504 |
| LweI | GCATC | 1 cut(s) | 274 |
| MaeIII | GTNAC | 1 cut(s) | 237 |
| MalI | GATC | 2 cut(s) | 199, 210 |
| MboI | GATC | 2 cut(s) | 197, 208 |
| MboII | GAAGA | 4 cut(s) | 127, 158, 193, 444 |
| MfeI | CAATTG | 1 cut(s) | 141 |
| MhlI | GDGCHC | 3 cut(s) | 300, 439, 499 |
| MluCI | AATT | 4 cut(s) | 141, 274, 399, 606 |
| MlyI | GAGTC | 2 cut(s) | 443, 459 |
| MmeI | TCCRAC | 1 cut(s) | 125 |
| MnlI | CCTC | 3 cut(s) | 55, 301, 479 |
| MseI | TTAA | 1 cut(s) | 264 |
| MslI | CAYNNNNRTG | 2 cut(s) | 126, 246 |
| MspA1I | CMGCKG | 1 cut(s) | 214 |
| MspR9I | CCNGG | 2 cut(s) | 473, 602 |
| MunI | CAATTG | 1 cut(s) | 141 |
| MvaI | CCWGG | 2 cut(s) | 473, 602 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 71, 104 |
| NdeII | GATC | 2 cut(s) | 197, 208 |
| NlaIII | CATG | 9 cut(s) | 45, 55, 125, 226, 307, 367, 443, 629, 634 |
| NspI | RCATGY | 1 cut(s) | 125 |
| PagI | TCATGA | 1 cut(s) | 222 |
| PfeI | GAWTC | 1 cut(s) | 21 |
| PkrI | GCNGC | 3 cut(s) | 97, 519, 535 |
| PleI | GAGTC | 2 cut(s) | 443, 459 |
| PpsI | GAGTC | 2 cut(s) | 443, 459 |
| Psp124BI | GAGCTC | 1 cut(s) | 439 |
| Psp6I | CCWGG | 2 cut(s) | 471, 600 |
| PspGI | CCWGG | 2 cut(s) | 471, 600 |
| PspPI | GGNCC | 1 cut(s) | 622 |
| RsaI | GTAC | 3 cut(s) | 162, 189, 585 |
| RsaNI | GTAC | 3 cut(s) | 161, 188, 584 |
| RseI | CAYNNNNRTG | 2 cut(s) | 126, 246 |
| SacI | GAGCTC | 1 cut(s) | 439 |
| SaqAI | TTAA | 1 cut(s) | 264 |
| SatI | GCNGC | 3 cut(s) | 96, 518, 534 |
| Sau3AI | GATC | 2 cut(s) | 197, 208 |
| Sau96I | GGNCC | 1 cut(s) | 622 |
| ScaI | AGTACT | 1 cut(s) | 162 |
| SchI | GAGTC | 2 cut(s) | 443, 459 |
| ScrFI | CCNGG | 2 cut(s) | 473, 602 |
| SduI | GDGCHC | 3 cut(s) | 300, 439, 499 |
| SetI | ASST | 8 cut(s) | 72, 315, 427, 439, 519, 555, 573, 606 |
| SfaNI | GCATC | 1 cut(s) | 274 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 126, 246 |
| SmlI | CTYRAG | 1 cut(s) | 407 |
| SmoI | CTYRAG | 1 cut(s) | 407 |
| Sse9I | AATT | 4 cut(s) | 141, 274, 399, 606 |
| SsiI | CCGC | 3 cut(s) | 214, 476, 533 |
| SstI | GAGCTC | 1 cut(s) | 439 |
| StyD4I | CCNGG | 2 cut(s) | 471, 600 |
| TaaI | ACNGT | 2 cut(s) | 10, 545 |
| TaqI | TCGA | 3 cut(s) | 113, 318, 342 |
| TasI | AATT | 4 cut(s) | 141, 274, 399, 606 |
| TatI | WGTACW | 3 cut(s) | 160, 187, 583 |
| TauI | GCSGC | 1 cut(s) | 536 |
| TfiI | GAWTC | 1 cut(s) | 21 |
| Tru1I | TTAA | 1 cut(s) | 264 |
| Tru9I | TTAA | 1 cut(s) | 264 |
| TscAI | CASTG | 1 cut(s) | 396 |
| TseI | GCWGC | 2 cut(s) | 95, 517 |
| TspDTI | ATGAA | 4 cut(s) | 70, 123, 352, 581 |
| TspRI | CASTG | 1 cut(s) | 396 |
| VneI | GTGCAC | 1 cut(s) | 495 |
| XapI | RAATTY | 1 cut(s) | 399 |
| XceI | RCATGY | 1 cut(s) | 125 |
| ZrmI | AGTACT | 1 cut(s) | 162 |