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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 30 |
| Alw26I | GTCTC | 1 cut(s) | 151 |
| BcoDI | GTCTC | 1 cut(s) | 151 |
| BmsI | GCATC | 1 cut(s) | 109 |
| BslFI | GGGAC | 1 cut(s) | 18 |
| BsmAI | GTCTC | 1 cut(s) | 151 |
| BsmFI | GGGAC | 1 cut(s) | 18 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 52 |
| BstC8I | GCNNGC | 3 cut(s) | 48, 57, 168 |
| BstMAI | GTCTC | 1 cut(s) | 151 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 52, 56 |
| BstNSI | RCATGY | 2 cut(s) | 50, 83 |
| Cac8I | GCNNGC | 3 cut(s) | 48, 57, 168 |
| CviAII | CATG | 3 cut(s) | 47, 80, 161 |
| CviJI | RGCY | 1 cut(s) | 166 |
| CviKI_1 | RGCY | 1 cut(s) | 166 |
| FaeI | CATG | 3 cut(s) | 50, 83, 164 |
| FaiI | YATR | 6 cut(s) | 48, 81, 92, 102, 129, 162 |
| FaqI | GGGAC | 1 cut(s) | 18 |
| FatI | CATG | 3 cut(s) | 46, 79, 160 |
| Hin1II | CATG | 3 cut(s) | 50, 83, 164 |
| Hpy188III | TCNNGA | 1 cut(s) | 116 |
| HpyAV | CCTTC | 1 cut(s) | 62 |
| HpyCH4V | TGCA | 3 cut(s) | 11, 55, 122 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 52, 56 |
| Hsp92II | CATG | 3 cut(s) | 50, 83, 164 |
| LpnPI | CCDG | 1 cut(s) | 41 |
| LweI | GCATC | 1 cut(s) | 109 |
| MluCI | AATT | 2 cut(s) | 36, 97 |
| MnlI | CCTC | 1 cut(s) | 136 |
| MseI | TTAA | 2 cut(s) | 63, 147 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 52, 56 |
| NlaIII | CATG | 3 cut(s) | 50, 83, 164 |
| NspI | RCATGY | 2 cut(s) | 50, 83 |
| PaeI | GCATGC | 1 cut(s) | 50 |
| SaqAI | TTAA | 2 cut(s) | 63, 147 |
| SetI | ASST | 2 cut(s) | 73, 147 |
| SfaNI | GCATC | 1 cut(s) | 109 |
| SgeI | CNNG | 8 cut(s) | 20, 24, 59, 68, 92, 121, 128, 164 |
| SphI | GCATGC | 1 cut(s) | 50 |
| Sse9I | AATT | 2 cut(s) | 36, 97 |
| TasI | AATT | 2 cut(s) | 36, 97 |
| Tru1I | TTAA | 2 cut(s) | 63, 147 |
| Tru9I | TTAA | 2 cut(s) | 63, 147 |
| TspDTI | ATGAA | 1 cut(s) | 117 |
| XceI | RCATGY | 2 cut(s) | 50, 83 |