Rorug06G0004700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000090
Physical Location & Seq
Reverse (-)
646880 .. 651230
4351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0004700.1

Sequence Viewer

Length: 1920 bp
ATGAGGTTTTCTGCATTGACTGTCGTCGATGAACTTGAGGACGTCGAAACCGTCGTCGTACTGGCAGGGATATCGATGCTCCGGCGCGAGGCGGTAGTTGACGGAGAGGACGACGGCGGGAAGCTCGCGAGCGAGGCGAAGAAGCTGAAGAGGACCGTCAAGGAAAGATTTCCCAGAGCTTCCGACGCCGATATCGATGCTTTACTCCCTCCCAAGGTGGAGATAAAGGTTTCAAAGTATCAAAATCGAGTCCTTGTATATGGCATAGAGGGAGGCTTTCCTATGTTTTTTGATATTGATGGGCGTGGCAAAGAAATTTTTGCCACAGTTTATGCCCTCTGGAAGGTCCCTGATCTCTTGCCTTCTTTCATGCTGAAAGGGAGCGAGGTTTCTCGATATGTTATTGGAGGGGCAGATTTGATGTTTCCCGGCATCAGCATACCTGCTGAAGGTTTACCATCGTTTATAGCTGGGGAAGCATGGGCGGTTAAAGTTCCAGGAAATCCAGCTCCCATTGCTGTGGGAACTACTACTATGAGCTGCACTGAAGCATTAAAAGCTGGATTGCGTGGAAAAGCCTTAAAAATTGCTCACTATTATGGTGACTTACTTTGGGAATCAGTTGAGGGCCCTTATGTGCCAAATGCAGGTTTCTTAGAAGATGCTGTTTTTGAGGATCCTACCTTGTCATCAGCTCAAATTTCTGATTCATATAATGGTGCAAATGATGGATCACATGATCAACTGAATGGCATTGATGATAAGGAAATGGGGCAACATGTTGATGTTGCTGATGTGCAGTCTGAACCAAGTTCTGCTTCGGTTGCACCGATTGATGTTAGGAATGAGATTGCTGAGGAAGTAACTGCAAATGTAGGTGAACTGAAGCTAACAGATGATGATTCTGCTAAACAGTCGACTGGGGAGGATCAACATCCTCTTTCTGCCGAGGATGTGGACATGCTTTTGGAAAAATGCCTTTTGCAAGCATTGCATACAACTGTCAAAGACAAAGACCTTCCCATGCCCGGAAGCACTCTATGGTCAAACTATGTTTTACCTTGTAGGCCTTCAGGCATCACACTAGACATCAAGAAATCATCACACAAGAAATTATCAAAGTGGTTACAAGCTAAATGTTCTACAGGATTGATTAGAGTGAAGGAAGATAAATATAAGAAGGAGTCTGTATTACTTTCTATTGACCGCAGCCATCCAGATTACTCATCTTTCAAGCCAGAGAAACGACAAGTTGAAGAAGCTGTTCAAACTGGTGTTCCTGCTGTCAGTGAAAGTCGGTCGGTCAAAATCCTAGAAGTGGGTGAGATCTATAAACCAAGTGTACATGTAAATCCAGTATTTGCTTCTCTAGGTGCTGACACAGGCGAGCTTTACAGTGCTTCCGATGCCACTGACATTGTGTTTAAATATTTTGAAAAGGAAAACCTGGTTAAGCCGACAGATAAATCCATTGTGATTTTGGATGCGATATTGTGTGATGCACTGTTCAAGGGGGCCATAAAGAAAGGTTCAACGTACCCTACAGAAATTCATAAAAGAGACTTAGGAGCAGCATTTGTGAACCGGATGCAAGCCCATCACGTTGTGACAAGGGGAAATGACTCGGTTGTTCGTAAAGGTGGTTTGAAAACAGTCCAGATAATGACAGAAAGGCGGCAAGGTAATAAGAAAATGACCAAGTTATCAGGACTGGAAACATTTTTAGTGGATCCTGAAGCTTTGGCATCAGAGCTACAAAAAAAGTTCGCTTGTAGTACAACGGTGGCAGAACTACCAGGTAAGAAGGGGCTGGAGGTTCTGGTTCAAGGTGGAGTGATTGAGAATCTGGCAAAACACTTGATTGAACAATATGGGATCCCAAAGAGATACATTGAAGTTCTTGACAAAACCAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_24

Protein Analysis

639

Amino Acids

69.82

Weight (kDa)

5.59

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pre-PUA PF17832 44 - 117 4.8e-13 Pre-PUA-like domain
PUA_elF2D PF26292 128 - 202 5.6e-22 elF2D, PUA domain
WHD_eIF2D PF25304 320 - 398 1.3e-28 eIF2D winged helix domain
SWIB_eIF2D PF26291 444 - 504 7.3e-11 eIF2D, SWIB domain
SUI1 PF01253 550 - 627 8.4e-22 Translation initiation factor SUI1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18340 FvH4_2g08421 FvH4_3g05430 FvH4_3g26318 FvH4_3g43970 FvH4_5g24831 FvH4_6g20331 FvH4_6g23072 FvH4_7g02471 FvH4_7g02601 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790
malus_domestica MD09G1277700.v1.1
pyrus_communis pycom14g05970 pycom395g00100
rosa_chinensis RchiOBHm_Chr1g0329081 RchiOBHm_Chr1g0332631 RchiOBHm_Chr1g0341051 RchiOBHm_Chr1g0346821 RchiOBHm_Chr1g0346841 RchiOBHm_Chr1g0377301 RchiOBHm_Chr1g0378881 RchiOBHm_Chr1g0381361 RchiOBHm_Chr2g0094421 RchiOBHm_Chr2g0115791 RchiOBHm_Chr2g0131391 RchiOBHm_Chr3g0469161 RchiOBHm_Chr3g0477491 RchiOBHm_Chr4g0397121 RchiOBHm_Chr4g0403221 RchiOBHm_Chr4g0403811 RchiOBHm_Chr4g0418081 RchiOBHm_Chr4g0434301 RchiOBHm_Chr5g0004171 RchiOBHm_Chr5g0007651 RchiOBHm_Chr5g0025151 RchiOBHm_Chr5g0029021 RchiOBHm_Chr5g0036581 RchiOBHm_Chr5g0040531 RchiOBHm_Chr5g0041581 RchiOBHm_Chr5g0041591 RchiOBHm_Chr5g0055171 RchiOBHm_Chr5g0059931 RchiOBHm_Chr5g0070861 RchiOBHm_Chr5g0072491 RchiOBHm_Chr5g0073831 RchiOBHm_Chr5g0076701 RchiOBHm_Chr6g0264941 RchiOBHm_Chr6g0268711 RchiOBHm_Chr6g0272081 RchiOBHm_Chr7g0180801 RchiOBHm_Chr7g0206041 RchiOBHm_Chr7g0206051 RchiOBHm_Chr7g0206191 RchiOBHm_Chr7g0207401 RchiOBHm_Chr7g0208561 RchiOBHm_Chr7g0208571 RchiOBHm_Chr7g0232661 RchiOBHm_Chr7g0238901
rosa_laevigata RLG00000003152 RLG00000007513 RLG00000010392 RLG00000011803 RLG00000012560 RLG00000014764 RLG00000017712 RLG00000017809 RLG00000017860 RLG00000018875 RLG00000020741 RLG00000021294 RLG00000021737 RLG00000023224 RLG00000023340 RLG00000025142 RLG00000027945 RLG00000028772 RLG00000028775 RLG00000028776 RLG00000029536 RLG00000029993 RLG00000030256 RLG00000030594 RLG00000030697 RLG00000032707 RLG00000035344 RLG00000035458 RLG00000036306 RLG00000036953
rosa_multiflora Rmu_co8065394.1_g000001 Rmu_co8424083.1_g000001 Rmu_sc0000012.1_g000007 Rmu_sc0000073.1_g000003 Rmu_sc0000177.1_g000022 Rmu_sc0000356.1_g000009 Rmu_sc0000433.1_g000025 Rmu_sc0001229.1_g000008 Rmu_sc0001478.1_g000007 Rmu_sc0001552.1_g000046 Rmu_sc0001731.1_g000001 Rmu_sc0001731.1_g000004 Rmu_sc0001942.1_g000088 Rmu_sc0002530.1_g000034 Rmu_sc0002758.1_g000006 Rmu_sc0003033.1_g000034 Rmu_sc0003158.1_g000009 Rmu_sc0003193.1_g000001 Rmu_sc0003314.1_g000041 Rmu_sc0003756.1_g000007 Rmu_sc0003895.1_g000016 Rmu_sc0004149.1_g000005 Rmu_sc0004413.1_g000038 Rmu_sc0004509.1_g000025 Rmu_sc0005142.1_g000008 Rmu_sc0005291.1_g000005 Rmu_sc0005772.1_g000001 Rmu_sc0005962.1_g000002 Rmu_sc0006223.1_g000037 Rmu_sc0006898.1_g000018 Rmu_sc0007822.1_g000006 Rmu_sc0007822.1_g000007 Rmu_sc0015982.1_g000001 Rmu_sc0023577.1_g000001 Rmu_sc0026009.1_g000013 Rmu_ssc0000264.1_g000011 Rmu_ssc0000264.1_g000014 Rmu_ssc0000467.1_g000035
rosa_roxburghii Rroxscaffold_1G00003310 Rroxscaffold_1G00003440 Rroxscaffold_1G00025390 Rroxscaffold_1G00027650 Rroxscaffold_1G00035520 Rroxscaffold_1G00039720 Rroxscaffold_2G00096880 Rroxscaffold_2G00138110 Rroxscaffold_3G00223540 Rroxscaffold_3G00226400 Rroxscaffold_3G00249970 Rroxscaffold_3G00251230 Rroxscaffold_4G00308110 Rroxscaffold_4G00308440 Rroxscaffold_4G00308470 Rroxscaffold_4G00311680 Rroxscaffold_4G00311690 Rroxscaffold_4G00330900 Rroxscaffold_5G00342170 Rroxscaffold_5G00376290 Rroxscaffold_6G00392450 Rroxscaffold_6G00421400 Rroxscaffold_6G00430840 Rroxscaffold_7G00171590 Rroxscaffold_7G00178520 Rroxscaffold_7G00192720
rosa_rugosa Rorug01G0184200 Rorug02G0001800 Rorug02G0296300.1 Rorug02G0296400 Rorug02G0395800 Rorug03G0045600 Rorug03G0045700 Rorug03G0273100 Rorug04G0060900 Rorug04G0073900 Rorug04G0082100 Rorug04G0083700 Rorug04G0140600 Rorug04G0147600 Rorug04G0202000 Rorug04G0365300 Rorug05G0164600 Rorug05G0164700 Rorug05G0164800 Rorug05G0313400 Rorug05G0460400 Rorug05G0460500 Rorug06G0004700 Rorug06G0087800 Rorug07G0040400 Rorug07G0147000 Rorug07G0164100 Rorug07G0183900 Rorug07G0313600
rosa_samantha Rh1AG151200 Rh1AG190200 Rh1AG201300 Rh1AG409200 Rh1BG069600 Rh1BG167900 Rh1BG168100 Rh1BG221200 Rh1BG231700 Rh1BG279700 Rh1BG335200 Rh1CG100500 Rh1CG142400 Rh1CG175600 Rh1CG186200 Rh1CG348700 Rh1DG025200 Rh1DG040900 Rh1DG107900 Rh1DG108000 Rh1DG132100 Rh1DG198700 Rh1DG198900 Rh1DG248100 Rh1DG311000 Rh1DG366700 Rh2AG005300 Rh2AG262100 Rh2CG263100 Rh2CG313000 Rh2CG313100 Rh2CG335400 Rh2CG392500 Rh2CG576000 Rh2DG091000 Rh3AG032000 Rh3BG032400 Rh3CG031100 Rh3CG163600 Rh3CG311100 Rh3DG032000 Rh4AG091500 Rh4BG086500 Rh4BG111300 Rh4BG139900 Rh4CG015000 Rh4CG099800 Rh4DG210900 Rh4DG234600 Rh4DG282500 Rh5AG075100 Rh5AG228600 Rh5AG326300 Rh5AG464100 Rh5BG265900 Rh5BG284000 Rh5BG284900 Rh5CG337600 Rh5CG394700 Rh5DG206500 Rh5DG285000 Rh5DG293700 Rh5DG403900 Rh5DG469800 Rh6AG045500 Rh6AG242800 Rh6BG073100 Rh6BG202400 Rh6CG179100 Rh6CG202800 Rh6DG170800 Rh6DG194300 Rh6DG440100 Rh6DG445600 Rh6DG470700 Rh7BG262200 Rh7BG295500 Rh7BG295800 Rh7CG210600 Rh7CG421500 Rh7DG233400 Rh7DG233500 Rh7DG238100 Rh7DG238200 Rh7DG245500
rosa_wichuraiana Rw1G016920 Rw1G022060 Rw1G028030 Rw2G007760 Rw3G016140 Rw3G020210 Rw4G012320 Rw4G026900 Rw4G034750 Rw5G028220 Rw6G003840 Rw6G004130 Rw7G032360 Rw7G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 45
Acc36I ACCTGC 2 cut(s) 451, 638
AccI GTMKAC 1 cut(s) 917
AccII CGCG 2 cut(s) 87, 128
AciI CCGC 5 cut(s) 92, 117, 485, 1207, 1675
AclWI GGATC 8 cut(s) 671, 684, 739, 936, 1724, 1737, 1870, 1883
AcsI RAATTY 3 cut(s) 315, 699, 1548
AcuI CTGAAG 6 cut(s) 167, 468, 567, 905, 1056, 1755
AcyI GRCGYC 2 cut(s) 42, 186
AdeI CACNNNGTG 1 cut(s) 1606
AfaI GTAC 4 cut(s) 60, 1344, 1538, 1777
AfiI CCNNNNNNNGG 7 cut(s) 88, 214, 343, 449, 647, 1028, 1318
AflIII ACRYGT 2 cut(s) 778, 1345
AjnI CCWGG 4 cut(s) 496, 1446, 1795, 1910
AjuI GAANNNNNNNTTGG 2 cut(s) 802, 834
Alw26I GTCTC 1 cut(s) 1554
AlwI GGATC 8 cut(s) 671, 684, 739, 936, 1724, 1737, 1870, 1883
AoxI GGCC 3 cut(s) 628, 1067, 1515
ApaI GGGCCC 1 cut(s) 632
ApeKI GCWGC 3 cut(s) 540, 1209, 1571
ApoI RAATTY 3 cut(s) 315, 699, 1548
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
Asp700I GAANNNNTTC 2 cut(s) 168, 1263
AspLEI GCGC 1 cut(s) 87
AspS9I GGNCC 5 cut(s) 153, 346, 628, 629, 1515
AsuC2I CCSGG 2 cut(s) 429, 1029
AsuHPI GGTGA 3 cut(s) 614, 890, 1334
AvaII GGWCC 2 cut(s) 153, 346
BaeGI GKGCMC 1 cut(s) 632
BamHI GGATCC 3 cut(s) 676, 1729, 1875
BanII GRGCYC 1 cut(s) 632
BbvCI CCTCAGC 1 cut(s) 855
BbvI GCAGC 3 cut(s) 527, 1221, 1583
BccI CCATC 5 cut(s) 293, 466, 722, 1221, 1605
BceAI ACGGC 1 cut(s) 130
BcgI CGANNNNNNTGC 4 cut(s) 54, 88, 179, 213
BciT130I CCWGG 4 cut(s) 498, 1448, 1797, 1912
BclI TGATCA 1 cut(s) 739
BcnI CCSGG 2 cut(s) 429, 1029
BcoDI GTCTC 1 cut(s) 1554
BfaI CTAG 3 cut(s) 1085, 1313, 1370
BfmI CTRYAG 2 cut(s) 1143, 1542
BfuAI ACCTGC 2 cut(s) 451, 638
BglII AGATCT 1 cut(s) 1326
BisI GCNGC 4 cut(s) 541, 1210, 1572, 1676
BlsI GCNGC 4 cut(s) 542, 1211, 1573, 1677
Bme1390I CCNGG 6 cut(s) 429, 498, 1029, 1448, 1797, 1912
Bme18I GGWCC 2 cut(s) 153, 346
BmgT120I GGNCC 5 cut(s) 153, 346, 628, 629, 1515
BmiI GGNNCC 6 cut(s) 348, 630, 678, 1516, 1731, 1877
BmrFI CCNGG 6 cut(s) 429, 498, 1029, 1448, 1797, 1912
BmrI ACTGGG 1 cut(s) 930
BmuI ACTGGG 1 cut(s) 930
BoxI GACNNNNGTC 1 cut(s) 23
BpmI CTGGAG 1 cut(s) 1832
Bpu10I CCTNAGC 1 cut(s) 855
BpuEI CTTGAG 1 cut(s) 56
BpuMI CCSGG 2 cut(s) 429, 1029
Bsa29I ATCGAT 2 cut(s) 74, 195
BsaBI GATNNNNATC 1 cut(s) 933
BsaHI GRCGYC 2 cut(s) 42, 186
BsaJI CCNNGG 2 cut(s) 213, 948
BsaWI WCCGGW 1 cut(s) 1584
Bsc4I CCNNNNNNNGG 7 cut(s) 88, 214, 343, 449, 647, 1028, 1318
Bse1I ACTGG 5 cut(s) 66, 925, 1276, 1355, 1716
Bse3DI GCAATG 2 cut(s) 513, 989
Bse8I GATNNNNATC 1 cut(s) 933
BseBI CCWGG 4 cut(s) 498, 1448, 1797, 1912
BseCI ATCGAT 2 cut(s) 74, 195
BseDI CCNNGG 2 cut(s) 213, 948
BseGI GGATG 5 cut(s) 934, 958, 1213, 1489, 1593
BseJI GATNNNNATC 1 cut(s) 933
BseLI CCNNNNNNNGG 7 cut(s) 88, 214, 343, 449, 647, 1028, 1318
BseMI GCAATG 2 cut(s) 513, 989
BseMII CTCAG 1 cut(s) 846
BseNI ACTGG 5 cut(s) 66, 925, 1276, 1355, 1716
BseSI GKGCMC 1 cut(s) 632
BseXI GCAGC 3 cut(s) 527, 1221, 1583
BseYI CCCAGC 1 cut(s) 470
BsgI GTGCAG 2 cut(s) 526, 818
Bsh1236I CGCG 2 cut(s) 87, 128
Bsh1285I CGRYCG 1 cut(s) 1301
BshFI GGCC 3 cut(s) 630, 1069, 1517
BshVI ATCGAT 2 cut(s) 74, 195
BsiEI CGRYCG 1 cut(s) 1301
BsiSI CCGG 4 cut(s) 82, 429, 1029, 1585
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 7 cut(s) 88, 214, 343, 449, 647, 1028, 1318
BsmAI GTCTC 1 cut(s) 1554
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 3 cut(s) 630, 1069, 1517
Bsp120I GGGCCC 1 cut(s) 628
Bsp1286I GDGCHC 1 cut(s) 632
Bsp1407I TGTACA 1 cut(s) 1342
Bsp143I GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
Bsp68I TCGCGA 1 cut(s) 128
BspACI CCGC 5 cut(s) 92, 117, 485, 1207, 1675
BspANI GGCC 3 cut(s) 630, 1069, 1517
BspCNI CTCAG 1 cut(s) 847
BspDI ATCGAT 2 cut(s) 74, 195
BspFNI CGCG 2 cut(s) 87, 128
BspLI GGNNCC 6 cut(s) 348, 630, 678, 1516, 1731, 1877
BspMI ACCTGC 2 cut(s) 451, 638
BspPI GGATC 8 cut(s) 671, 684, 739, 936, 1724, 1737, 1870, 1883
BsrDI GCAATG 2 cut(s) 513, 989
BsrGI TGTACA 1 cut(s) 1342
BsrI ACTGG 5 cut(s) 66, 925, 1276, 1355, 1716
BssECI CCNNGG 2 cut(s) 213, 948
BssMI GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
BssNI GRCGYC 2 cut(s) 42, 186
BssT1I CCWWGG 1 cut(s) 213
Bst2UI CCWGG 4 cut(s) 498, 1448, 1797, 1912
Bst6I CTCTTC 1 cut(s) 143
BstACI GRCGYC 2 cut(s) 42, 186
BstAPI GCANNNNNTGC 1 cut(s) 991
BstAUI TGTACA 1 cut(s) 1342
BstC8I GCNNGC 5 cut(s) 126, 130, 987, 1388, 1593
BstDEI CTNAG 3 cut(s) 655, 855, 1564
BstENI CCTNNNNNAGG 2 cut(s) 341, 447
BstF5I GGATG 5 cut(s) 934, 958, 1213, 1489, 1593
BstFNI CGCG 2 cut(s) 87, 128
BstHHI GCGC 1 cut(s) 87
BstKTI GATC 8 cut(s) 355, 679, 734, 742, 931, 1329, 1732, 1878
BstMAI GTCTC 1 cut(s) 1554
BstMBI GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
BstMCI CGRYCG 1 cut(s) 1301
BstMWI GCNNNNNNNGC 8 cut(s) 134, 185, 476, 515, 557, 824, 991, 1406
BstNI CCWGG 4 cut(s) 498, 1448, 1797, 1912
BstNSI RCATGY 3 cut(s) 782, 964, 1349
BstPAI GACNNNNGTC 1 cut(s) 23
BstSCI CCNGG 6 cut(s) 427, 496, 1027, 1446, 1795, 1910
BstSFI CTRYAG 2 cut(s) 1143, 1542
BstSLI GKGCMC 1 cut(s) 632
BstUI CGCG 2 cut(s) 87, 128
BstV1I GCAGC 3 cut(s) 527, 1221, 1583
BstX2I RGATCY 4 cut(s) 676, 1326, 1729, 1875
BstXI CCANNNNNNTGG 1 cut(s) 520
BstYI RGATCY 4 cut(s) 676, 1326, 1729, 1875
Bsu15I ATCGAT 2 cut(s) 74, 195
BsuRI GGCC 3 cut(s) 630, 1069, 1517
BsuTUI ATCGAT 2 cut(s) 74, 195
BtsCI GGATG 5 cut(s) 934, 958, 1213, 1489, 1593
BtsIMutI CAGTG 5 cut(s) 543, 1294, 1402, 1410, 1502
BtuMI TCGCGA 1 cut(s) 128
BveI ACCTGC 2 cut(s) 451, 638
Cac8I GCNNGC 5 cut(s) 126, 130, 987, 1388, 1593
CfoI GCGC 1 cut(s) 87
Cfr13I GGNCC 5 cut(s) 153, 346, 628, 629, 1515
ClaI ATCGAT 2 cut(s) 74, 195
CseI GACGC 1 cut(s) 194
CsiI ACCWGGT 2 cut(s) 1446, 1795
Csp6I GTAC 4 cut(s) 59, 1343, 1537, 1776
CspCI CAANNNNNGTGG 2 cut(s) 1399, 1434
CviAII CATG 7 cut(s) 370, 480, 737, 779, 961, 1024, 1346
CviQI GTAC 4 cut(s) 59, 1343, 1537, 1776
DdeI CTNAG 3 cut(s) 655, 855, 1564
DpnI GATC 8 cut(s) 354, 678, 733, 741, 930, 1328, 1731, 1877
DpnII GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
DraI TTTAAA 1 cut(s) 1426
DraIII CACNNNGTG 1 cut(s) 1606
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 213
Eco147I AGGCCT 1 cut(s) 1069
Eco24I GRGCYC 1 cut(s) 632
Eco32I GATATC 2 cut(s) 72, 193
Eco47I GGWCC 2 cut(s) 153, 346
Eco57I CTGAAG 6 cut(s) 167, 468, 567, 905, 1056, 1755
EcoNI CCTNNNNNAGG 2 cut(s) 341, 447
EcoO109I RGGNCCY 3 cut(s) 346, 628, 629
EcoRII CCWGG 4 cut(s) 496, 1446, 1795, 1910
EcoRV GATATC 2 cut(s) 72, 193
EcoT14I CCWWGG 1 cut(s) 213
EcoT38I GRGCYC 1 cut(s) 632
ErhI CCWWGG 1 cut(s) 213
FaeI CATG 7 cut(s) 373, 483, 740, 782, 964, 1027, 1349
FalI AAGNNNNNCTT 2 cut(s) 802, 834
FaqI GGGAC 1 cut(s) 332
FatI CATG 7 cut(s) 369, 479, 736, 778, 960, 1023, 1345
FauI CCCGC 1 cut(s) 110
FbaI TGATCA 1 cut(s) 739
FblI GTMKAC 1 cut(s) 917
Fnu4HI GCNGC 4 cut(s) 541, 1210, 1572, 1676
FokI GGATG 5 cut(s) 921, 965, 1200, 1496, 1600
FriOI GRGCYC 1 cut(s) 632
Fsp4HI GCNGC 4 cut(s) 541, 1210, 1572, 1676
FspBI CTAG 3 cut(s) 1085, 1313, 1370
GlaI GCGC 1 cut(s) 86
GluI GCNGC 4 cut(s) 541, 1210, 1572, 1676
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 1 cut(s) 1832
HaeIII GGCC 3 cut(s) 630, 1069, 1517
HapII CCGG 4 cut(s) 82, 429, 1029, 1585
HgaI GACGC 1 cut(s) 194
HhaI GCGC 1 cut(s) 87
Hin1I GRCGYC 2 cut(s) 42, 186
Hin1II CATG 7 cut(s) 373, 483, 740, 782, 964, 1027, 1349
Hin6I GCGC 1 cut(s) 85
HinP1I GCGC 1 cut(s) 85
HincII GTYRAC 2 cut(s) 100, 918
HindII GTYRAC 2 cut(s) 100, 918
HindIII AAGCTT 1 cut(s) 1737
HinfI GANTC 7 cut(s) 249, 617, 707, 902, 1184, 1622, 1843
HpaII CCGG 4 cut(s) 82, 429, 1029, 1585
HphI GGTGA 3 cut(s) 614, 890, 1334
Hpy166II GTNNAC 7 cut(s) 100, 455, 881, 918, 958, 1343, 1582
Hpy188I TCNGA 5 cut(s) 184, 706, 805, 1405, 1750
Hpy188III TCNNGA 9 cut(s) 127, 340, 393, 1093, 1217, 1657, 1707, 1733, 1901
Hpy8I GTNNAC 7 cut(s) 100, 455, 881, 918, 958, 1343, 1582
Hpy99I CGWCG 6 cut(s) 29, 47, 56, 59, 116, 188
HpyAV CCTTC 8 cut(s) 337, 372, 443, 1028, 1080, 1156, 1174, 1798
HpyCH4IV ACGT 3 cut(s) 42, 1535, 1602
HpyF10VI GCNNNNNNNGC 8 cut(s) 134, 185, 476, 515, 557, 824, 991, 1406
HpyF3I CTNAG 3 cut(s) 655, 855, 1564
HpySE526I ACGT 3 cut(s) 42, 1535, 1602
Hsp92I GRCGYC 2 cut(s) 42, 186
Hsp92II CATG 7 cut(s) 373, 483, 740, 782, 964, 1027, 1349
HspAI GCGC 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 739
Kzo9I GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
LmnI GCTCC 4 cut(s) 84, 381, 514, 1568
Lsp1109I GCAGC 3 cut(s) 527, 1221, 1583
MabI ACCWGGT 2 cut(s) 1446, 1795
MaeI CTAG 3 cut(s) 1085, 1313, 1370
MaeII ACGT 3 cut(s) 42, 1535, 1602
MaeIII GTNAC 4 cut(s) 602, 862, 1125, 1606
MalI GATC 8 cut(s) 354, 678, 733, 741, 930, 1328, 1731, 1877
MboI GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
MboII GAAGA 5 cut(s) 151, 160, 671, 1178, 1268
MflI RGATCY 4 cut(s) 676, 1326, 1729, 1875
MhlI GDGCHC 1 cut(s) 632
MluCI AATT 5 cut(s) 315, 585, 699, 1112, 1548
MlyI GAGTC 3 cut(s) 258, 1193, 1616
MmeI TCCRAC 1 cut(s) 207
MroXI GAANNNNTTC 2 cut(s) 168, 1263
MseI TTAA 5 cut(s) 489, 554, 581, 1425, 1452
MslI CAYNNNNRTG 3 cut(s) 518, 597, 783
MspI CCGG 4 cut(s) 82, 429, 1029, 1585
MspR9I CCNGG 6 cut(s) 429, 498, 1029, 1448, 1797, 1912
MvaI CCWGG 4 cut(s) 498, 1448, 1797, 1912
MvnI CGCG 2 cut(s) 87, 128
MwoI GCNNNNNNNGC 8 cut(s) 134, 185, 476, 515, 557, 824, 991, 1406
NciI CCSGG 2 cut(s) 429, 1029
NdeII GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
NlaIII CATG 7 cut(s) 373, 483, 740, 782, 964, 1027, 1349
NlaIV GGNNCC 6 cut(s) 348, 630, 678, 1516, 1731, 1877
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 2 cut(s) 602, 1606
NruI TCGCGA 1 cut(s) 128
NspI RCATGY 3 cut(s) 782, 964, 1349
PceI AGGCCT 1 cut(s) 1069
PciI ACATGT 2 cut(s) 778, 1345
PcsI WCGNNNNNNNCGW 5 cut(s) 48, 51, 108, 192, 827
PdmI GAANNNNTTC 2 cut(s) 168, 1263
PfeI GAWTC 4 cut(s) 617, 707, 902, 1843
PfoI TCCNGGA 1 cut(s) 496
PkrI GCNGC 4 cut(s) 542, 1211, 1573, 1677
PleI GAGTC 3 cut(s) 257, 1192, 1616
PpsI GAGTC 3 cut(s) 257, 1192, 1616
PpuMI RGGWCCY 1 cut(s) 346
PscI ACATGT 2 cut(s) 778, 1345
PshAI GACNNNNGTC 1 cut(s) 23
Psp5II RGGWCCY 1 cut(s) 346
Psp6I CCWGG 4 cut(s) 496, 1446, 1795, 1910
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 4 cut(s) 496, 1446, 1795, 1910
PspN4I GGNNCC 6 cut(s) 348, 630, 678, 1516, 1731, 1877
PspOMI GGGCCC 1 cut(s) 628
PspPI GGNCC 5 cut(s) 153, 346, 628, 629, 1515
PspPPI RGGWCCY 1 cut(s) 346
PsuI RGATCY 4 cut(s) 676, 1326, 1729, 1875
RruI TCGCGA 1 cut(s) 128
RsaI GTAC 4 cut(s) 60, 1344, 1538, 1777
RsaNI GTAC 4 cut(s) 59, 1343, 1537, 1776
RseI CAYNNNNRTG 3 cut(s) 518, 597, 783
SalI GTCGAC 1 cut(s) 916
SaqAI TTAA 5 cut(s) 489, 554, 581, 1425, 1452
SatI GCNGC 4 cut(s) 541, 1210, 1572, 1676
Sau3AI GATC 8 cut(s) 352, 676, 731, 739, 928, 1326, 1729, 1875
Sau96I GGNCC 5 cut(s) 153, 346, 628, 629, 1515
SchI GAGTC 3 cut(s) 258, 1193, 1616
ScrFI CCNGG 6 cut(s) 429, 498, 1029, 1448, 1797, 1912
SduI GDGCHC 1 cut(s) 632
SexAI ACCWGGT 2 cut(s) 1446, 1795
SfcI CTRYAG 2 cut(s) 1143, 1542
SinI GGWCC 2 cut(s) 153, 346
SmiMI CAYNNNNRTG 3 cut(s) 518, 597, 783
SmlI CTYRAG 1 cut(s) 35
SmoI CTYRAG 1 cut(s) 35
Sse9I AATT 5 cut(s) 315, 585, 699, 1112, 1548
SseBI AGGCCT 1 cut(s) 1069
SsiI CCGC 5 cut(s) 92, 117, 485, 1207, 1675
SspI AATATT 1 cut(s) 1430
SspMI CTAG 3 cut(s) 1085, 1313, 1370
StuI AGGCCT 1 cut(s) 1069
StyD4I CCNGG 6 cut(s) 427, 496, 1027, 1446, 1795, 1910
StyI CCWWGG 1 cut(s) 213
TaiI ACGT 3 cut(s) 45, 1538, 1605
TaqI TCGA 7 cut(s) 27, 45, 74, 195, 247, 394, 917
TaqII GACCGA 2 cut(s) 1287, 1291
TasI AATT 5 cut(s) 315, 585, 699, 1112, 1548
TatI WGTACW 2 cut(s) 1342, 1775
TauI GCSGC 1 cut(s) 1678
TfiI GAWTC 4 cut(s) 617, 707, 902, 1843
Tru1I TTAA 5 cut(s) 489, 554, 581, 1425, 1452
Tru9I TTAA 5 cut(s) 489, 554, 581, 1425, 1452
TscAI CASTG 5 cut(s) 550, 1294, 1402, 1417, 1509
TseFI GTSAC 2 cut(s) 602, 1606
TseI GCWGC 3 cut(s) 540, 1209, 1571
Tsp45I GTSAC 2 cut(s) 602, 1606
TspDTI ATGAA 4 cut(s) 45, 358, 699, 1541
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 5 cut(s) 550, 1294, 1402, 1417, 1509
VpaK11BI GGWCC 2 cut(s) 153, 346
XagI CCTNNNNNAGG 2 cut(s) 341, 447
XapI RAATTY 3 cut(s) 315, 699, 1548
XceI RCATGY 3 cut(s) 782, 964, 1349
XcmI CCANNNNNNNNNTGG 1 cut(s) 1477
XmiI GTMKAC 1 cut(s) 917
XmnI GAANNNNTTC 2 cut(s) 168, 1263
XspI CTAG 3 cut(s) 1085, 1313, 1370
ZraI GACGTC 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.