RLG00000028772

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000090
Physical Location & Seq
Reverse (-)
27739387 .. 27744509
5123 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028772

Sequence Viewer

Length: 1524 bp
ATGGAAAGGCTTCATGAAAGCACACAATTTTTGGCTTCTCTCCGTTGCTCCCTTTCGCCGGAGAACCCATCTCCGACCGCCACAGACCTCTCTCCTTTTCAGTTTCTCTCGCCGATCTCTCCTTTCCTGTTCTCTGCAACACCTCCAGCCCCAAATCACCGTCCCGGCCTCACGCCACGAGCCTCAGTCATTTTGCTCCTCTTTCTTAGTAGGATGTTGCAATCAACAACAGTGGGCAAGAACAAGAAGAGGAAGTCAACAAAGGCAAGCCAGTCACGCCCACAAACACGTCTACAATCACAAGGACAGTCAGAACATGCATTCGTCACCCCAACTAGGGGGCAAATAGAAATTTTTGGTGAAGCATGTCAGACTGTTTCTTACGCACAGGCCCGGAAAACCCGTGGCATTACACAGGGGTTGGGAACTCAAGCTCTGATCAATGCTTGCAAGCAGAGGATTCCAATCCGTACGAATGGTGAGCACAAGCTCCCCCAGAATGTTCGAGCCAATATTAAGTTTAGGTCAGAGATTGGGATACAAACGCGTCAGAATGCTCCCCTTATGGTCAAGCGCTGGAAAGAAGTCTCTGCTTCGGATAAGGAAAAAATCACCAAGGCCTTGGCGATTAAGTTTGATTTTGATGGTACGGACCCGGTTGTAAGGAAATTTATTGAGGCTCATATGTCCAAGTCGTACTCACTATGGAGGAGCAGGCTGAACAAACATTACAAGCTGCATGCTTATGACCCTGAATATGCACGAGCACACCCTCCCGTAAAGCTGTTTTATAATAGAGAAATGGCTGAATGGGAGTGGTTGTGTGATGAATTATTCACAGATGAGGACTATCAGAAACGTTGCAAGATAAACTCTGCTAACCGGAATAAGCAAGAATACGATCATCTTGAGAGTTTACCACCCTACCCGAAACATATAGCAGCTGCACAAGAGATGCCAGTAGAATCAATAAAAAAGGATTCTGAGTATGATGATGATGAGGTTGATGTTGATGATAATGGTAATGATGATGATGATGATGTTGATTATAATGTTGATGATGATTATGTTGGTTATAATGATCATGTTGATGCTAATGATGAGGATGATGTTGCTGATAATGTTGGAAGAAACCTAGAAGTTGAGGTTGATAATGTTGAAGAGCGTGGAAATCAAAGTGTATCCACCAATACTGAGAAAAGTAGTGGAAAGAGAAAGCTCTCATCCTTTACCAATGTGCATGGTGAGAGTTCAAAGAAGAGACGTGAAATAGCTGAGCAAAGATTTACTGATTCTGCACCTCTGCGTGGTAATGATGAGGTTCAATCAGTTTCAAAGGAGGTTAGCGATGAAGAATACTTGATGCAATGTTTTGGAATTTTGAACAAGATGGACGAAATTGATGATGACAGTTACTCAAAAGCCTTGAAGCTCTTCCGCGGCGATGCTACTTGGAGAAAGCTGTTTGTGGGAATGCCGGAGAAGAGGAGAAAGAACTTCATCCTCAATCTTTTCTGTACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

508

Amino Acids

57.98

Weight (kDa)

6.13

Isoelectric Point (pI)

57.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18340 FvH4_2g08421 FvH4_3g05430 FvH4_3g26318 FvH4_3g43970 FvH4_5g24831 FvH4_6g20331 FvH4_6g23072 FvH4_7g02471 FvH4_7g02601 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790
malus_domestica MD09G1277700.v1.1
pyrus_communis pycom14g05970 pycom395g00100
rosa_chinensis RchiOBHm_Chr1g0329081 RchiOBHm_Chr1g0332631 RchiOBHm_Chr1g0341051 RchiOBHm_Chr1g0346821 RchiOBHm_Chr1g0346841 RchiOBHm_Chr1g0377301 RchiOBHm_Chr1g0378881 RchiOBHm_Chr1g0381361 RchiOBHm_Chr2g0094421 RchiOBHm_Chr2g0115791 RchiOBHm_Chr2g0131391 RchiOBHm_Chr3g0469161 RchiOBHm_Chr3g0477491 RchiOBHm_Chr4g0397121 RchiOBHm_Chr4g0403221 RchiOBHm_Chr4g0403811 RchiOBHm_Chr4g0418081 RchiOBHm_Chr4g0434301 RchiOBHm_Chr5g0004171 RchiOBHm_Chr5g0007651 RchiOBHm_Chr5g0025151 RchiOBHm_Chr5g0029021 RchiOBHm_Chr5g0036581 RchiOBHm_Chr5g0040531 RchiOBHm_Chr5g0041581 RchiOBHm_Chr5g0041591 RchiOBHm_Chr5g0055171 RchiOBHm_Chr5g0059931 RchiOBHm_Chr5g0070861 RchiOBHm_Chr5g0072491 RchiOBHm_Chr5g0073831 RchiOBHm_Chr5g0076701 RchiOBHm_Chr6g0264941 RchiOBHm_Chr6g0268711 RchiOBHm_Chr6g0272081 RchiOBHm_Chr7g0180801 RchiOBHm_Chr7g0206041 RchiOBHm_Chr7g0206051 RchiOBHm_Chr7g0206191 RchiOBHm_Chr7g0207401 RchiOBHm_Chr7g0208561 RchiOBHm_Chr7g0208571 RchiOBHm_Chr7g0232661 RchiOBHm_Chr7g0238901
rosa_laevigata RLG00000003152 RLG00000007513 RLG00000010392 RLG00000011803 RLG00000012560 RLG00000014764 RLG00000017712 RLG00000017809 RLG00000017860 RLG00000018875 RLG00000020741 RLG00000021294 RLG00000021737 RLG00000023224 RLG00000023340 RLG00000025142 RLG00000027945 RLG00000028772 RLG00000028775 RLG00000028776 RLG00000029536 RLG00000029993 RLG00000030256 RLG00000030594 RLG00000030697 RLG00000032707 RLG00000035344 RLG00000035458 RLG00000036306 RLG00000036953
rosa_multiflora Rmu_co8065394.1_g000001 Rmu_co8424083.1_g000001 Rmu_sc0000012.1_g000007 Rmu_sc0000073.1_g000003 Rmu_sc0000177.1_g000022 Rmu_sc0000356.1_g000009 Rmu_sc0000433.1_g000025 Rmu_sc0001229.1_g000008 Rmu_sc0001478.1_g000007 Rmu_sc0001552.1_g000046 Rmu_sc0001731.1_g000001 Rmu_sc0001731.1_g000004 Rmu_sc0001942.1_g000088 Rmu_sc0002530.1_g000034 Rmu_sc0002758.1_g000006 Rmu_sc0003033.1_g000034 Rmu_sc0003158.1_g000009 Rmu_sc0003193.1_g000001 Rmu_sc0003314.1_g000041 Rmu_sc0003756.1_g000007 Rmu_sc0003895.1_g000016 Rmu_sc0004149.1_g000005 Rmu_sc0004413.1_g000038 Rmu_sc0004509.1_g000025 Rmu_sc0005142.1_g000008 Rmu_sc0005291.1_g000005 Rmu_sc0005772.1_g000001 Rmu_sc0005962.1_g000002 Rmu_sc0006223.1_g000037 Rmu_sc0006898.1_g000018 Rmu_sc0007822.1_g000006 Rmu_sc0007822.1_g000007 Rmu_sc0015982.1_g000001 Rmu_sc0023577.1_g000001 Rmu_sc0026009.1_g000013 Rmu_ssc0000264.1_g000011 Rmu_ssc0000264.1_g000014 Rmu_ssc0000467.1_g000035
rosa_roxburghii Rroxscaffold_1G00003310 Rroxscaffold_1G00003440 Rroxscaffold_1G00025390 Rroxscaffold_1G00027650 Rroxscaffold_1G00035520 Rroxscaffold_1G00039720 Rroxscaffold_2G00096880 Rroxscaffold_2G00138110 Rroxscaffold_3G00223540 Rroxscaffold_3G00226400 Rroxscaffold_3G00249970 Rroxscaffold_3G00251230 Rroxscaffold_4G00308110 Rroxscaffold_4G00308440 Rroxscaffold_4G00308470 Rroxscaffold_4G00311680 Rroxscaffold_4G00311690 Rroxscaffold_4G00330900 Rroxscaffold_5G00342170 Rroxscaffold_5G00376290 Rroxscaffold_6G00392450 Rroxscaffold_6G00421400 Rroxscaffold_6G00430840 Rroxscaffold_7G00171590 Rroxscaffold_7G00178520 Rroxscaffold_7G00192720
rosa_rugosa Rorug01G0184200 Rorug02G0001800 Rorug02G0296300.1 Rorug02G0296400 Rorug02G0395800 Rorug03G0045600 Rorug03G0045700 Rorug03G0273100 Rorug04G0060900 Rorug04G0073900 Rorug04G0082100 Rorug04G0083700 Rorug04G0140600 Rorug04G0147600 Rorug04G0202000 Rorug04G0365300 Rorug05G0164600 Rorug05G0164700 Rorug05G0164800 Rorug05G0313400 Rorug05G0460400 Rorug05G0460500 Rorug06G0004700 Rorug06G0087800 Rorug07G0040400 Rorug07G0147000 Rorug07G0164100 Rorug07G0183900 Rorug07G0313600
rosa_samantha Rh1AG151200 Rh1AG190200 Rh1AG201300 Rh1AG409200 Rh1BG069600 Rh1BG167900 Rh1BG168100 Rh1BG221200 Rh1BG231700 Rh1BG279700 Rh1BG335200 Rh1CG100500 Rh1CG142400 Rh1CG175600 Rh1CG186200 Rh1CG348700 Rh1DG025200 Rh1DG040900 Rh1DG107900 Rh1DG108000 Rh1DG132100 Rh1DG198700 Rh1DG198900 Rh1DG248100 Rh1DG311000 Rh1DG366700 Rh2AG005300 Rh2AG262100 Rh2CG263100 Rh2CG313000 Rh2CG313100 Rh2CG335400 Rh2CG392500 Rh2CG576000 Rh2DG091000 Rh3AG032000 Rh3BG032400 Rh3CG031100 Rh3CG163600 Rh3CG311100 Rh3DG032000 Rh4AG091500 Rh4BG086500 Rh4BG111300 Rh4BG139900 Rh4CG015000 Rh4CG099800 Rh4DG210900 Rh4DG234600 Rh4DG282500 Rh5AG075100 Rh5AG228600 Rh5AG326300 Rh5AG464100 Rh5BG265900 Rh5BG284000 Rh5BG284900 Rh5CG337600 Rh5CG394700 Rh5DG206500 Rh5DG285000 Rh5DG293700 Rh5DG403900 Rh5DG469800 Rh6AG045500 Rh6AG242800 Rh6BG073100 Rh6BG202400 Rh6CG179100 Rh6CG202800 Rh6DG170800 Rh6DG194300 Rh6DG440100 Rh6DG445600 Rh6DG470700 Rh7BG262200 Rh7BG295500 Rh7BG295800 Rh7CG210600 Rh7CG421500 Rh7DG233400 Rh7DG233500 Rh7DG238100 Rh7DG238200 Rh7DG245500
rosa_wichuraiana Rw1G016920 Rw1G022060 Rw1G028030 Rw2G007760 Rw3G016140 Rw3G020210 Rw4G012320 Rw4G026900 Rw4G034750 Rw5G028220 Rw6G003840 Rw6G004130 Rw7G032360 Rw7G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 792, 1050, 1077
AccI GTMKAC 1 cut(s) 292
AccII CGCG 2 cut(s) 547, 1440
AciI CCGC 3 cut(s) 78, 1438, 1440
AclI AACGTT 1 cut(s) 859
AcsI RAATTY 3 cut(s) 351, 668, 1377
AfaI GTAC 4 cut(s) 472, 649, 698, 1519
AfeI AGCGCT 1 cut(s) 575
AfiI CCNNNNNNNGG 5 cut(s) 58, 336, 337, 338, 1307
AflIII ACRYGT 2 cut(s) 287, 545
AgsI TTSAA 6 cut(s) 1160, 1254, 1325, 1335, 1384, 1429
AjiI CACGTC 2 cut(s) 290, 1265
AluBI AGCT 9 cut(s) 434, 490, 736, 784, 944, 1219, 1274, 1432, 1462
AluI AGCT 9 cut(s) 434, 490, 736, 784, 944, 1219, 1274, 1432, 1462
Alw21I GWGCWC 2 cut(s) 486, 769
Alw26I GTCTC 2 cut(s) 592, 1255
Aor51HI AGCGCT 1 cut(s) 575
AoxI GGCC 3 cut(s) 166, 390, 618
ApeKI GCWGC 3 cut(s) 736, 941, 944
ApoI RAATTY 3 cut(s) 351, 668, 1377
Asp700I GAANNNNTTC 2 cut(s) 9, 1433
AspLEI GCGC 1 cut(s) 576
AspS9I GGNCC 2 cut(s) 391, 652
AsuC2I CCSGG 3 cut(s) 165, 394, 656
AsuHPI GGTGA 6 cut(s) 149, 319, 371, 491, 604, 1256
AvaII GGWCC 1 cut(s) 652
BauI CACGAG 2 cut(s) 177, 762
Bbv12I GWGCWC 2 cut(s) 486, 769
BbvI GCAGC 3 cut(s) 723, 931, 953
BccI CCATC 3 cut(s) 76, 638, 1384
BciVI GTATCC 2 cut(s) 531, 1192
BclI TGATCA 2 cut(s) 438, 1081
BcnI CCSGG 3 cut(s) 165, 394, 656
BcoDI GTCTC 2 cut(s) 592, 1255
BfaI CTAG 2 cut(s) 336, 1136
BfoI RGCGCY 1 cut(s) 577
BfuI GTATCC 2 cut(s) 531, 1192
BisI GCNGC 4 cut(s) 737, 942, 945, 1441
BlpI GCTNAGC 1 cut(s) 1275
BlsI GCNGC 4 cut(s) 738, 943, 946, 1442
Bme1390I CCNGG 3 cut(s) 165, 394, 656
Bme18I GGWCC 1 cut(s) 652
BmgBI CACGTC 2 cut(s) 290, 1265
BmgT120I GGNCC 2 cut(s) 391, 652
BmiI GGNNCC 1 cut(s) 654
BmrFI CCNGG 3 cut(s) 165, 394, 656
BmsI GCATC 4 cut(s) 945, 1081, 1353, 1435
BpmI CTGGAG 1 cut(s) 129
Bpu1102I GCTNAGC 1 cut(s) 1275
BpuEI CTTGAG 2 cut(s) 414, 929
BpuMI CCSGG 3 cut(s) 165, 394, 656
BsaBI GATNNNNATC 1 cut(s) 464
BsaJI CCNNGG 4 cut(s) 403, 615, 621, 1438
BsaWI WCCGGW 1 cut(s) 882
BsaXI ACNNNNNCTCC 2 cut(s) 806, 836
Bsc4I CCNNNNNNNGG 5 cut(s) 58, 336, 337, 338, 1307
Bse1I ACTGG 2 cut(s) 271, 959
Bse3DI GCAATG 1 cut(s) 1373
Bse8I GATNNNNATC 1 cut(s) 464
BseDI CCNNGG 4 cut(s) 403, 615, 621, 1438
BseGI GGATG 4 cut(s) 219, 1111, 1223, 1500
BseJI GATNNNNATC 1 cut(s) 464
BseLI CCNNNNNNNGG 5 cut(s) 58, 336, 337, 338, 1307
BseMI GCAATG 1 cut(s) 1373
BseMII CTCAG 4 cut(s) 198, 975, 1185, 1266
BseNI ACTGG 2 cut(s) 271, 959
BseRI GAGGAG 3 cut(s) 188, 724, 1501
BseXI GCAGC 3 cut(s) 723, 931, 953
BsgI GTGCAG 2 cut(s) 930, 1281
Bsh1236I CGCG 2 cut(s) 547, 1440
Bsh1285I CGRYCG 1 cut(s) 78
BshFI GGCC 3 cut(s) 168, 392, 620
BsiEI CGRYCG 1 cut(s) 78
BsiHKAI GWGCWC 2 cut(s) 486, 769
BsiSI CCGG 6 cut(s) 59, 165, 394, 656, 883, 1478
BsiWI CGTACG 1 cut(s) 470
BslFI GGGAC 1 cut(s) 147
BslI CCNNNNNNNGG 5 cut(s) 58, 336, 337, 338, 1307
BsmAI GTCTC 2 cut(s) 592, 1255
BsmBI CGTCTC 1 cut(s) 1255
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 3 cut(s) 320, 559, 1479
BsnI GGCC 3 cut(s) 168, 392, 620
Bsp1286I GDGCHC 2 cut(s) 486, 769
Bsp1407I TGTACA 1 cut(s) 1517
Bsp143I GATC 4 cut(s) 114, 438, 901, 1081
Bsp1720I GCTNAGC 1 cut(s) 1275
BspACI CCGC 3 cut(s) 78, 1438, 1440
BspANI GGCC 3 cut(s) 168, 392, 620
BspCNI CTCAG 4 cut(s) 197, 976, 1186, 1267
BspFNI CGCG 2 cut(s) 547, 1440
BspHI TCATGA 1 cut(s) 13
BspLI GGNNCC 1 cut(s) 654
BspQI GCTCTTC 2 cut(s) 1155, 1439
BsrDI GCAATG 1 cut(s) 1373
BsrGI TGTACA 1 cut(s) 1517
BsrI ACTGG 2 cut(s) 271, 959
BssECI CCNNGG 4 cut(s) 403, 615, 621, 1438
BssMI GATC 4 cut(s) 114, 438, 901, 1081
BssSI CACGAG 2 cut(s) 177, 762
BssT1I CCWWGG 2 cut(s) 615, 621
Bst2BI CACGAG 2 cut(s) 177, 762
Bst4CI ACNGT 5 cut(s) 161, 232, 309, 376, 1412
Bst6I CTCTTC 5 cut(s) 242, 1155, 1253, 1439, 1478
BstAUI TGTACA 1 cut(s) 1517
BstC8I GCNNGC 5 cut(s) 268, 448, 452, 716, 741
BstDEI CTNAG 5 cut(s) 184, 206, 984, 1194, 1275
BstDSI CCRYGG 2 cut(s) 403, 1438
BstF5I GGATG 4 cut(s) 219, 1111, 1223, 1500
BstFNI CGCG 2 cut(s) 547, 1440
BstH2I RGCGCY 1 cut(s) 577
BstHHI GCGC 1 cut(s) 576
BstKTI GATC 4 cut(s) 117, 441, 904, 1084
BstMAI GTCTC 2 cut(s) 592, 1255
BstMBI GATC 4 cut(s) 114, 438, 901, 1081
BstMCI CGRYCG 1 cut(s) 78
BstMWI GCNNNNNNNGC 1 cut(s) 276
BstNSI RCATGY 3 cut(s) 320, 369, 743
BstSCI CCNGG 3 cut(s) 163, 392, 654
BstUI CGCG 2 cut(s) 547, 1440
BstV1I GCAGC 3 cut(s) 723, 931, 953
BstXI CCANNNNNNTGG 1 cut(s) 622
BsuI GTATCC 2 cut(s) 531, 1192
BsuRI GGCC 3 cut(s) 168, 392, 620
BtgI CCRYGG 2 cut(s) 403, 1438
BtgZI GCGATG 2 cut(s) 1362, 1458
BtrI CACGTC 2 cut(s) 290, 1265
BtsCI GGATG 4 cut(s) 219, 1111, 1223, 1500
BtsIMutI CAGTG 1 cut(s) 237
Cac8I GCNNGC 5 cut(s) 268, 448, 452, 716, 741
CciI TCATGA 1 cut(s) 13
CfoI GCGC 1 cut(s) 576
Cfr13I GGNCC 2 cut(s) 391, 652
Cfr42I CCGCGG 1 cut(s) 1441
CseI GACGC 1 cut(s) 536
Csp6I GTAC 4 cut(s) 471, 648, 697, 1518
CspCI CAANNNNNGTGG 2 cut(s) 213, 248
CviAII CATG 7 cut(s) 14, 317, 366, 740, 1085, 1241, 1521
CviQI GTAC 4 cut(s) 471, 648, 697, 1518
DdeI CTNAG 5 cut(s) 184, 206, 984, 1194, 1275
DpnI GATC 4 cut(s) 116, 440, 903, 1083
DpnII GATC 4 cut(s) 114, 438, 901, 1081
Eam1104I CTCTTC 5 cut(s) 242, 1155, 1253, 1439, 1478
EarI CTCTTC 5 cut(s) 242, 1155, 1253, 1439, 1478
Eco130I CCWWGG 2 cut(s) 615, 621
Eco147I AGGCCT 1 cut(s) 620
Eco47I GGWCC 1 cut(s) 652
Eco47III AGCGCT 1 cut(s) 575
EcoT14I CCWWGG 2 cut(s) 615, 621
EcoT22I ATGCAT 1 cut(s) 322
ErhI CCWWGG 2 cut(s) 615, 621
Esp3I CGTCTC 1 cut(s) 1255
FaeI CATG 7 cut(s) 17, 320, 369, 743, 1088, 1244, 1524
FaqI GGGAC 1 cut(s) 147
FatI CATG 7 cut(s) 13, 316, 365, 739, 1084, 1240, 1520
FauNDI CATATG 1 cut(s) 684
FbaI TGATCA 2 cut(s) 438, 1081
FblI GTMKAC 1 cut(s) 292
Fnu4HI GCNGC 4 cut(s) 737, 942, 945, 1441
FokI GGATG 4 cut(s) 226, 1118, 1210, 1487
Fsp4HI GCNGC 4 cut(s) 737, 942, 945, 1441
FspBI CTAG 2 cut(s) 336, 1136
GlaI GCGC 1 cut(s) 575
GluI GCNGC 4 cut(s) 737, 942, 945, 1441
GsuI CTGGAG 1 cut(s) 129
HaeII RGCGCY 1 cut(s) 577
HaeIII GGCC 3 cut(s) 168, 392, 620
HapII CCGG 6 cut(s) 59, 165, 394, 656, 883, 1478
HgaI GACGC 1 cut(s) 536
HhaI GCGC 1 cut(s) 576
Hin1II CATG 7 cut(s) 17, 320, 369, 743, 1088, 1244, 1524
Hin6I GCGC 1 cut(s) 574
HinP1I GCGC 1 cut(s) 574
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HinfI GANTC 4 cut(s) 460, 965, 980, 1292
HpaII CCGG 6 cut(s) 59, 165, 394, 656, 883, 1478
HphI GGTGA 6 cut(s) 149, 319, 371, 491, 604, 1256
Hpy166II GTNNAC 3 cut(s) 258, 293, 917
Hpy188I TCNGA 9 cut(s) 75, 313, 372, 438, 529, 552, 598, 855, 985
Hpy188III TCNNGA 2 cut(s) 14, 908
Hpy8I GTNNAC 3 cut(s) 258, 293, 917
HpyCH4III ACNGT 5 cut(s) 161, 232, 309, 376, 1412
HpyCH4IV ACGT 3 cut(s) 289, 859, 1264
HpyF10VI GCNNNNNNNGC 1 cut(s) 276
HpyF3I CTNAG 5 cut(s) 184, 206, 984, 1194, 1275
HpySE526I ACGT 3 cut(s) 289, 859, 1264
Hsp92II CATG 7 cut(s) 17, 320, 369, 743, 1088, 1244, 1524
HspAI GCGC 1 cut(s) 574
Ksp22I TGATCA 2 cut(s) 438, 1081
KspI CCGCGG 1 cut(s) 1441
Kzo9I GATC 4 cut(s) 114, 438, 901, 1081
LguI GCTCTTC 2 cut(s) 1155, 1439
LmnI GCTCC 5 cut(s) 53, 201, 495, 562, 711
Lsp1109I GCAGC 3 cut(s) 723, 931, 953
LweI GCATC 4 cut(s) 945, 1081, 1353, 1435
MaeI CTAG 2 cut(s) 336, 1136
MaeII ACGT 3 cut(s) 289, 859, 1264
MaeIII GTNAC 3 cut(s) 273, 325, 1412
MalI GATC 4 cut(s) 116, 440, 903, 1083
MboI GATC 4 cut(s) 114, 438, 901, 1081
MboII GAAGA 7 cut(s) 259, 1140, 1172, 1270, 1364, 1426, 1495
MhlI GDGCHC 2 cut(s) 486, 769
MluCI AATT 6 cut(s) 26, 351, 668, 830, 1377, 1398
MluI ACGCGT 1 cut(s) 545
MmeI TCCRAC 2 cut(s) 98, 1105
Mph1103I ATGCAT 1 cut(s) 322
MroXI GAANNNNTTC 2 cut(s) 9, 1433
MseI TTAA 2 cut(s) 516, 630
MslI CAYNNNNRTG 2 cut(s) 744, 1089
MspA1I CMGCKG 2 cut(s) 944, 1440
MspI CCGG 6 cut(s) 59, 165, 394, 656, 883, 1478
MspR9I CCNGG 3 cut(s) 165, 394, 656
Mva1269I GAATGC 3 cut(s) 320, 559, 1479
MvnI CGCG 2 cut(s) 547, 1440
MwoI GCNNNNNNNGC 1 cut(s) 276
NciI CCSGG 3 cut(s) 165, 394, 656
NdeI CATATG 1 cut(s) 684
NdeII GATC 4 cut(s) 114, 438, 901, 1081
NlaIII CATG 7 cut(s) 17, 320, 369, 743, 1088, 1244, 1524
NlaIV GGNNCC 1 cut(s) 654
NmuCI GTSAC 2 cut(s) 273, 325
NsiI ATGCAT 1 cut(s) 322
NspI RCATGY 3 cut(s) 320, 369, 743
PaeI GCATGC 1 cut(s) 743
PagI TCATGA 1 cut(s) 13
PceI AGGCCT 1 cut(s) 620
PciSI GCTCTTC 2 cut(s) 1155, 1439
PctI GAATGC 3 cut(s) 320, 559, 1479
PdmI GAANNNNTTC 2 cut(s) 9, 1433
PfeI GAWTC 4 cut(s) 460, 965, 980, 1292
Pfl23II CGTACG 1 cut(s) 470
PkrI GCNGC 4 cut(s) 738, 943, 946, 1442
PsiI TTATAA 3 cut(s) 792, 1050, 1077
Psp1406I AACGTT 1 cut(s) 859
PspLI CGTACG 1 cut(s) 470
PspN4I GGNNCC 1 cut(s) 654
PspPI GGNCC 2 cut(s) 391, 652
PsrI GAACNNNNNNTAC 2 cut(s) 713, 745
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 4 cut(s) 472, 649, 698, 1519
RsaNI GTAC 4 cut(s) 471, 648, 697, 1518
RseI CAYNNNNRTG 2 cut(s) 744, 1089
SacII CCGCGG 1 cut(s) 1441
SapI GCTCTTC 2 cut(s) 1155, 1439
SaqAI TTAA 2 cut(s) 516, 630
SatI GCNGC 4 cut(s) 737, 942, 945, 1441
Sau3AI GATC 4 cut(s) 114, 438, 901, 1081
Sau96I GGNCC 2 cut(s) 391, 652
ScrFI CCNGG 3 cut(s) 165, 394, 656
SduI GDGCHC 2 cut(s) 486, 769
SfaNI GCATC 4 cut(s) 945, 1081, 1353, 1435
Sfr303I CCGCGG 1 cut(s) 1441
SgrBI CCGCGG 1 cut(s) 1441
SinI GGWCC 1 cut(s) 652
SmiMI CAYNNNNRTG 2 cut(s) 744, 1089
SmlI CTYRAG 2 cut(s) 429, 908
SmoI CTYRAG 2 cut(s) 429, 908
SphI GCATGC 1 cut(s) 743
Sse9I AATT 6 cut(s) 26, 351, 668, 830, 1377, 1398
SseBI AGGCCT 1 cut(s) 620
SsiI CCGC 3 cut(s) 78, 1438, 1440
SspI AATATT 1 cut(s) 514
SspMI CTAG 2 cut(s) 336, 1136
StuI AGGCCT 1 cut(s) 620
StyD4I CCNGG 3 cut(s) 163, 392, 654
StyI CCWWGG 2 cut(s) 615, 621
TaaI ACNGT 5 cut(s) 161, 232, 309, 376, 1412
TaiI ACGT 3 cut(s) 292, 862, 1267
TaqI TCGA 1 cut(s) 505
TasI AATT 6 cut(s) 26, 351, 668, 830, 1377, 1398
TatI WGTACW 1 cut(s) 1517
TauI GCSGC 1 cut(s) 1443
TfiI GAWTC 4 cut(s) 460, 965, 980, 1292
Tru1I TTAA 2 cut(s) 516, 630
Tru9I TTAA 2 cut(s) 516, 630
TscAI CASTG 1 cut(s) 237
TseFI GTSAC 2 cut(s) 273, 325
TseI GCWGC 3 cut(s) 736, 941, 944
Tsp45I GTSAC 2 cut(s) 273, 325
TspDTI ATGAA 4 cut(s) 30, 843, 1365, 1489
TspGWI ACGGA 3 cut(s) 32, 458, 665
TspRI CASTG 1 cut(s) 237
VpaK11BI GGWCC 1 cut(s) 652
XapI RAATTY 3 cut(s) 351, 668, 1377
XceI RCATGY 3 cut(s) 320, 369, 743
XmiI GTMKAC 1 cut(s) 292
XmnI GAANNNNTTC 2 cut(s) 9, 1433
XspI CTAG 2 cut(s) 336, 1136
Zsp2I ATGCAT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.