Rmu_sc0001731.1_g000004

TMV resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001731.1
OG0000090
Physical Location & Seq
Forward (+)
10088 .. 13232
3145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001731.1_g000004.1.cds

Sequence Viewer

Length: 1302 bp
atgttgcaatcaacaacagtgggcaagaacaagaagaggaagtcaataaaggcaagccagccatacccacaaacacgtctgcaatcacaagcacagtcagaacatgcattcgtcaccccaactagggggcaaatagaaatttttggtgaagcaggtcagaatgtttcttatgcacaggccaggaaaacccgtggcattacacaggggttgggaactcaagctctgatcaatgcttgcaagcagaggatttcaatccgtacgaatggtgagcacaagctcccccagaatgttcgagccaatattaagtttaggtcagagattgggatacaaacgcgtcagaatgctccacttatggtcaagcgctggaaagaagtctctgcttcggataaggaaaaaatcaccaaggccttggcgattaaatttgattttgatggtacggacctggttgtaaggaaatttattgaggctcatatgtccaagtcatactcactatggaggagcaggctgaacaaacattacaagcagcatgcttatgaccctgaatatgcacgggcacaccctcccttaaagctgttttataatagagaaatggctgaatgggagtggttgtgtgatgaattattcacagatgaggactatcagaaacgttgcaagataaactctgctaaccggaataagcaagaatacggtcatcttgagagttcaccaccctacccgaaacatatagcagctgcacaggagacgccagtagaatcgacaaaaaaggattctgagtatgatgatgatgaggataatgttgatgataacggtaatgaggatgatgattataatgttgatgatgattatgttggttataatgatcgtgttgatgttaatgatgaggatgattctgatgataacgttggaagaaacctagaagttgaggttgataatgttgaagagcatggaaatcaaagtgtatccactaatactgagaaaagcagtggaaagagaaagctctcatcctttaccaatgtgcatgttgagagttcaaagaagagacatgaaatagctgagcaaagatttaccgattctgcacctctgcgtggtaatgatgaggttcaatcagtttcaaaggaggttagcgatgaagaatacctgatgcaatgttttggaattttgaataagatggacgaaattgatgatgacagttactcaaaagccttgaagctcttccgcggtgatgctacttggagaaagctgtttgtgggaatgcctgagaagaggagaaagaacttcatcctcaatcttttctgtacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

433

Amino Acids

49.89

Weight (kDa)

5.97

Isoelectric Point (pI)

54.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18340 FvH4_2g08421 FvH4_3g05430 FvH4_3g26318 FvH4_3g43970 FvH4_5g24831 FvH4_6g20331 FvH4_6g23072 FvH4_7g02471 FvH4_7g02601 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790
malus_domestica MD09G1277700.v1.1
pyrus_communis pycom14g05970 pycom395g00100
rosa_chinensis RchiOBHm_Chr1g0329081 RchiOBHm_Chr1g0332631 RchiOBHm_Chr1g0341051 RchiOBHm_Chr1g0346821 RchiOBHm_Chr1g0346841 RchiOBHm_Chr1g0377301 RchiOBHm_Chr1g0378881 RchiOBHm_Chr1g0381361 RchiOBHm_Chr2g0094421 RchiOBHm_Chr2g0115791 RchiOBHm_Chr2g0131391 RchiOBHm_Chr3g0469161 RchiOBHm_Chr3g0477491 RchiOBHm_Chr4g0397121 RchiOBHm_Chr4g0403221 RchiOBHm_Chr4g0403811 RchiOBHm_Chr4g0418081 RchiOBHm_Chr4g0434301 RchiOBHm_Chr5g0004171 RchiOBHm_Chr5g0007651 RchiOBHm_Chr5g0025151 RchiOBHm_Chr5g0029021 RchiOBHm_Chr5g0036581 RchiOBHm_Chr5g0040531 RchiOBHm_Chr5g0041581 RchiOBHm_Chr5g0041591 RchiOBHm_Chr5g0055171 RchiOBHm_Chr5g0059931 RchiOBHm_Chr5g0070861 RchiOBHm_Chr5g0072491 RchiOBHm_Chr5g0073831 RchiOBHm_Chr5g0076701 RchiOBHm_Chr6g0264941 RchiOBHm_Chr6g0268711 RchiOBHm_Chr6g0272081 RchiOBHm_Chr7g0180801 RchiOBHm_Chr7g0206041 RchiOBHm_Chr7g0206051 RchiOBHm_Chr7g0206191 RchiOBHm_Chr7g0207401 RchiOBHm_Chr7g0208561 RchiOBHm_Chr7g0208571 RchiOBHm_Chr7g0232661 RchiOBHm_Chr7g0238901
rosa_laevigata RLG00000003152 RLG00000007513 RLG00000010392 RLG00000011803 RLG00000012560 RLG00000014764 RLG00000017712 RLG00000017809 RLG00000017860 RLG00000018875 RLG00000020741 RLG00000021294 RLG00000021737 RLG00000023224 RLG00000023340 RLG00000025142 RLG00000027945 RLG00000028772 RLG00000028775 RLG00000028776 RLG00000029536 RLG00000029993 RLG00000030256 RLG00000030594 RLG00000030697 RLG00000032707 RLG00000035344 RLG00000035458 RLG00000036306 RLG00000036953
rosa_multiflora Rmu_co8065394.1_g000001 Rmu_co8424083.1_g000001 Rmu_sc0000012.1_g000007 Rmu_sc0000073.1_g000003 Rmu_sc0000177.1_g000022 Rmu_sc0000356.1_g000009 Rmu_sc0000433.1_g000025 Rmu_sc0001229.1_g000008 Rmu_sc0001478.1_g000007 Rmu_sc0001552.1_g000046 Rmu_sc0001731.1_g000001 Rmu_sc0001731.1_g000004 Rmu_sc0001942.1_g000088 Rmu_sc0002530.1_g000034 Rmu_sc0002758.1_g000006 Rmu_sc0003033.1_g000034 Rmu_sc0003158.1_g000009 Rmu_sc0003193.1_g000001 Rmu_sc0003314.1_g000041 Rmu_sc0003756.1_g000007 Rmu_sc0003895.1_g000016 Rmu_sc0004149.1_g000005 Rmu_sc0004413.1_g000038 Rmu_sc0004509.1_g000025 Rmu_sc0005142.1_g000008 Rmu_sc0005291.1_g000005 Rmu_sc0005772.1_g000001 Rmu_sc0005962.1_g000002 Rmu_sc0006223.1_g000037 Rmu_sc0006898.1_g000018 Rmu_sc0007822.1_g000006 Rmu_sc0007822.1_g000007 Rmu_sc0015982.1_g000001 Rmu_sc0023577.1_g000001 Rmu_sc0026009.1_g000013 Rmu_ssc0000264.1_g000011 Rmu_ssc0000264.1_g000014 Rmu_ssc0000467.1_g000035
rosa_roxburghii Rroxscaffold_1G00003310 Rroxscaffold_1G00003440 Rroxscaffold_1G00025390 Rroxscaffold_1G00027650 Rroxscaffold_1G00035520 Rroxscaffold_1G00039720 Rroxscaffold_2G00096880 Rroxscaffold_2G00138110 Rroxscaffold_3G00223540 Rroxscaffold_3G00226400 Rroxscaffold_3G00249970 Rroxscaffold_3G00251230 Rroxscaffold_4G00308110 Rroxscaffold_4G00308440 Rroxscaffold_4G00308470 Rroxscaffold_4G00311680 Rroxscaffold_4G00311690 Rroxscaffold_4G00330900 Rroxscaffold_5G00342170 Rroxscaffold_5G00376290 Rroxscaffold_6G00392450 Rroxscaffold_6G00421400 Rroxscaffold_6G00430840 Rroxscaffold_7G00171590 Rroxscaffold_7G00178520 Rroxscaffold_7G00192720
rosa_rugosa Rorug01G0184200 Rorug02G0001800 Rorug02G0296300.1 Rorug02G0296400 Rorug02G0395800 Rorug03G0045600 Rorug03G0045700 Rorug03G0273100 Rorug04G0060900 Rorug04G0073900 Rorug04G0082100 Rorug04G0083700 Rorug04G0140600 Rorug04G0147600 Rorug04G0202000 Rorug04G0365300 Rorug05G0164600 Rorug05G0164700 Rorug05G0164800 Rorug05G0313400 Rorug05G0460400 Rorug05G0460500 Rorug06G0004700 Rorug06G0087800 Rorug07G0040400 Rorug07G0147000 Rorug07G0164100 Rorug07G0183900 Rorug07G0313600
rosa_samantha Rh1AG151200 Rh1AG190200 Rh1AG201300 Rh1AG409200 Rh1BG069600 Rh1BG167900 Rh1BG168100 Rh1BG221200 Rh1BG231700 Rh1BG279700 Rh1BG335200 Rh1CG100500 Rh1CG142400 Rh1CG175600 Rh1CG186200 Rh1CG348700 Rh1DG025200 Rh1DG040900 Rh1DG107900 Rh1DG108000 Rh1DG132100 Rh1DG198700 Rh1DG198900 Rh1DG248100 Rh1DG311000 Rh1DG366700 Rh2AG005300 Rh2AG262100 Rh2CG263100 Rh2CG313000 Rh2CG313100 Rh2CG335400 Rh2CG392500 Rh2CG576000 Rh2DG091000 Rh3AG032000 Rh3BG032400 Rh3CG031100 Rh3CG163600 Rh3CG311100 Rh3DG032000 Rh4AG091500 Rh4BG086500 Rh4BG111300 Rh4BG139900 Rh4CG015000 Rh4CG099800 Rh4DG210900 Rh4DG234600 Rh4DG282500 Rh5AG075100 Rh5AG228600 Rh5AG326300 Rh5AG464100 Rh5BG265900 Rh5BG284000 Rh5BG284900 Rh5CG337600 Rh5CG394700 Rh5DG206500 Rh5DG285000 Rh5DG293700 Rh5DG403900 Rh5DG469800 Rh6AG045500 Rh6AG242800 Rh6BG073100 Rh6BG202400 Rh6CG179100 Rh6CG202800 Rh6DG170800 Rh6DG194300 Rh6DG440100 Rh6DG445600 Rh6DG470700 Rh7BG262200 Rh7BG295500 Rh7BG295800 Rh7CG210600 Rh7CG421500 Rh7DG233400 Rh7DG233500 Rh7DG238100 Rh7DG238200 Rh7DG245500
rosa_wichuraiana Rw1G016920 Rw1G022060 Rw1G028030 Rw2G007760 Rw3G016140 Rw3G020210 Rw4G012320 Rw4G026900 Rw4G034750 Rw5G028220 Rw6G003840 Rw6G004130 Rw7G032360 Rw7G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 579, 828, 855
Acc36I ACCTGC 1 cut(s) 143
AccII CGCG 2 cut(s) 334, 1218
AciI CCGC 2 cut(s) 1216, 1218
AclI AACGTT 2 cut(s) 646, 900
AcsI RAATTY 4 cut(s) 138, 419, 455, 1155
AcyI GRCGYC 1 cut(s) 743
AfaI GTAC 3 cut(s) 259, 436, 1297
AfeI AGCGCT 1 cut(s) 362
AfiI CCNNNNNNNGG 4 cut(s) 123, 124, 125, 1085
AflIII ACRYGT 2 cut(s) 74, 332
AgsI TTSAA 7 cut(s) 252, 938, 1032, 1103, 1113, 1162, 1207
AjiI CACGTC 1 cut(s) 77
AjnI CCWGG 2 cut(s) 179, 441
AluBI AGCT 8 cut(s) 221, 277, 571, 731, 997, 1052, 1210, 1240
AluI AGCT 8 cut(s) 221, 277, 571, 731, 997, 1052, 1210, 1240
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 3 cut(s) 379, 734, 1033
Aor51HI AGCGCT 1 cut(s) 362
AoxI GGCC 2 cut(s) 177, 405
ApeKI GCWGC 3 cut(s) 523, 728, 731
ApoI RAATTY 4 cut(s) 138, 419, 455, 1155
Asp700I GAANNNNTTC 1 cut(s) 1211
AspLEI GCGC 1 cut(s) 363
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 6 cut(s) 106, 158, 278, 391, 696, 1232
AvaII GGWCC 1 cut(s) 439
BaeGI GKGCMC 1 cut(s) 556
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 3 cut(s) 535, 718, 740
BccI CCATC 2 cut(s) 425, 1162
BciT130I CCWGG 2 cut(s) 181, 443
BciVI GTATCC 2 cut(s) 318, 970
BclI TGATCA 1 cut(s) 225
BcoDI GTCTC 3 cut(s) 379, 734, 1033
BfaI CTAG 2 cut(s) 123, 914
BfoI RGCGCY 1 cut(s) 364
BfuAI ACCTGC 1 cut(s) 143
BfuI GTATCC 2 cut(s) 318, 970
BisI GCNGC 3 cut(s) 524, 729, 732
BlpI GCTNAGC 1 cut(s) 1053
BlsI GCNGC 3 cut(s) 525, 730, 733
Bme1390I CCNGG 2 cut(s) 181, 443
Bme18I GGWCC 1 cut(s) 439
BmgBI CACGTC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 439
BmrFI CCNGG 2 cut(s) 181, 443
BmsI GCATC 2 cut(s) 1131, 1213
Bpu1102I GCTNAGC 1 cut(s) 1053
BpuEI CTTGAG 2 cut(s) 201, 716
BsaBI GATNNNNATC 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 743
BsaJI CCNNGG 4 cut(s) 190, 402, 408, 1216
BsaWI WCCGGW 1 cut(s) 669
BsaXI ACNNNNNCTCC 2 cut(s) 593, 623
Bsc4I CCNNNNNNNGG 4 cut(s) 123, 124, 125, 1085
Bse1I ACTGG 1 cut(s) 746
Bse3DI GCAATG 1 cut(s) 1151
Bse8I GATNNNNATC 1 cut(s) 251
BseBI CCWGG 2 cut(s) 181, 443
BseDI CCNNGG 4 cut(s) 190, 402, 408, 1216
BseGI GGATG 4 cut(s) 823, 889, 1001, 1278
BseJI GATNNNNATC 1 cut(s) 251
BseLI CCNNNNNNNGG 4 cut(s) 123, 124, 125, 1085
BseMI GCAATG 1 cut(s) 1151
BseMII CTCAG 4 cut(s) 762, 963, 1044, 1248
BseNI ACTGG 1 cut(s) 746
BseRI GAGGAG 2 cut(s) 511, 1279
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 3 cut(s) 535, 718, 740
BsgI GTGCAG 2 cut(s) 717, 1059
Bsh1236I CGCG 2 cut(s) 334, 1218
BshFI GGCC 2 cut(s) 179, 407
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 1 cut(s) 670
BsiWI CGTACG 1 cut(s) 257
BslI CCNNNNNNNGG 4 cut(s) 123, 124, 125, 1085
BsmAI GTCTC 3 cut(s) 379, 734, 1033
BsmBI CGTCTC 1 cut(s) 734
BsmI GAATGC 3 cut(s) 107, 346, 1257
BsnI GGCC 2 cut(s) 179, 407
Bsp1286I GDGCHC 2 cut(s) 273, 556
Bsp1407I TGTACA 1 cut(s) 1295
Bsp143I GATC 2 cut(s) 225, 859
Bsp1720I GCTNAGC 1 cut(s) 1053
BspACI CCGC 2 cut(s) 1216, 1218
BspANI GGCC 2 cut(s) 179, 407
BspCNI CTCAG 4 cut(s) 763, 964, 1045, 1249
BspFNI CGCG 2 cut(s) 334, 1218
BspMI ACCTGC 1 cut(s) 143
BspQI GCTCTTC 2 cut(s) 933, 1217
BsrDI GCAATG 1 cut(s) 1151
BsrGI TGTACA 1 cut(s) 1295
BsrI ACTGG 1 cut(s) 746
BssECI CCNNGG 4 cut(s) 190, 402, 408, 1216
BssMI GATC 2 cut(s) 225, 859
BssNI GRCGYC 1 cut(s) 743
BssT1I CCWWGG 2 cut(s) 402, 408
Bst2UI CCWGG 2 cut(s) 181, 443
Bst4CI ACNGT 5 cut(s) 19, 96, 689, 809, 1190
Bst6I CTCTTC 5 cut(s) 29, 933, 1031, 1217, 1256
BstACI GRCGYC 1 cut(s) 743
BstAUI TGTACA 1 cut(s) 1295
BstC8I GCNNGC 6 cut(s) 55, 59, 235, 239, 503, 528
BstDEI CTNAG 4 cut(s) 771, 972, 1053, 1257
BstDSI CCRYGG 2 cut(s) 190, 1216
BstF5I GGATG 4 cut(s) 823, 889, 1001, 1278
BstFNI CGCG 2 cut(s) 334, 1218
BstH2I RGCGCY 1 cut(s) 364
BstHHI GCGC 1 cut(s) 363
BstKTI GATC 2 cut(s) 228, 862
BstMAI GTCTC 3 cut(s) 379, 734, 1033
BstMBI GATC 2 cut(s) 225, 859
BstNI CCWGG 2 cut(s) 181, 443
BstNSI RCATGY 3 cut(s) 107, 530, 1022
BstSCI CCNGG 2 cut(s) 179, 441
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 2 cut(s) 334, 1218
BstV1I GCAGC 3 cut(s) 535, 718, 740
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuI GTATCC 2 cut(s) 318, 970
BsuRI GGCC 2 cut(s) 179, 407
BtgI CCRYGG 2 cut(s) 190, 1216
BtgZI GCGATG 1 cut(s) 1140
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 4 cut(s) 823, 889, 1001, 1278
BtsI GCAGTG 1 cut(s) 988
BtsIMutI CAGTG 2 cut(s) 24, 988
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 6 cut(s) 55, 59, 235, 239, 503, 528
CfoI GCGC 1 cut(s) 363
Cfr13I GGNCC 1 cut(s) 439
Cfr42I CCGCGG 1 cut(s) 1219
CseI GACGC 2 cut(s) 323, 751
CsiI ACCWGGT 1 cut(s) 441
Csp6I GTAC 3 cut(s) 258, 435, 1296
CspCI CAANNNNNGTGG 1 cut(s) 35
CviAII CATG 6 cut(s) 104, 527, 944, 1019, 1043, 1299
CviQI GTAC 3 cut(s) 258, 435, 1296
DdeI CTNAG 4 cut(s) 771, 972, 1053, 1257
DpnI GATC 2 cut(s) 227, 861
DpnII GATC 2 cut(s) 225, 859
Eam1104I CTCTTC 5 cut(s) 29, 933, 1031, 1217, 1256
EarI CTCTTC 5 cut(s) 29, 933, 1031, 1217, 1256
Eco130I CCWWGG 2 cut(s) 402, 408
Eco147I AGGCCT 1 cut(s) 407
Eco47I GGWCC 1 cut(s) 439
Eco47III AGCGCT 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 179, 441
EcoT14I CCWWGG 2 cut(s) 402, 408
EcoT22I ATGCAT 1 cut(s) 109
ErhI CCWWGG 2 cut(s) 402, 408
Esp3I CGTCTC 1 cut(s) 734
FaeI CATG 6 cut(s) 107, 530, 947, 1022, 1046, 1302
FatI CATG 6 cut(s) 103, 526, 943, 1018, 1042, 1298
FauNDI CATATG 1 cut(s) 471
FbaI TGATCA 1 cut(s) 225
Fnu4HI GCNGC 3 cut(s) 524, 729, 732
FokI GGATG 4 cut(s) 830, 896, 988, 1265
Fsp4HI GCNGC 3 cut(s) 524, 729, 732
FspBI CTAG 2 cut(s) 123, 914
GlaI GCGC 1 cut(s) 362
GluI GCNGC 3 cut(s) 524, 729, 732
HaeII RGCGCY 1 cut(s) 364
HaeIII GGCC 2 cut(s) 179, 407
HapII CCGG 1 cut(s) 670
HgaI GACGC 2 cut(s) 323, 751
HhaI GCGC 1 cut(s) 363
Hin1I GRCGYC 1 cut(s) 743
Hin1II CATG 6 cut(s) 107, 530, 947, 1022, 1046, 1302
Hin6I GCGC 1 cut(s) 361
HinP1I GCGC 1 cut(s) 361
HinfI GANTC 4 cut(s) 752, 767, 887, 1070
HpaII CCGG 1 cut(s) 670
HphI GGTGA 6 cut(s) 106, 158, 278, 391, 696, 1232
Hpy166II GTNNAC 1 cut(s) 704
Hpy188I TCNGA 9 cut(s) 100, 159, 225, 316, 339, 385, 642, 772, 892
Hpy188III TCNNGA 1 cut(s) 695
Hpy8I GTNNAC 1 cut(s) 704
HpyCH4III ACNGT 5 cut(s) 19, 96, 689, 809, 1190
HpyCH4IV ACGT 3 cut(s) 76, 646, 900
HpyF3I CTNAG 4 cut(s) 771, 972, 1053, 1257
HpySE526I ACGT 3 cut(s) 76, 646, 900
Hsp92I GRCGYC 1 cut(s) 743
Hsp92II CATG 6 cut(s) 107, 530, 947, 1022, 1046, 1302
HspAI GCGC 1 cut(s) 361
Ksp22I TGATCA 1 cut(s) 225
KspI CCGCGG 1 cut(s) 1219
Kzo9I GATC 2 cut(s) 225, 859
LguI GCTCTTC 2 cut(s) 933, 1217
LmnI GCTCC 3 cut(s) 282, 349, 498
Lsp1109I GCAGC 3 cut(s) 535, 718, 740
LweI GCATC 2 cut(s) 1131, 1213
MabI ACCWGGT 1 cut(s) 441
MaeI CTAG 2 cut(s) 123, 914
MaeII ACGT 3 cut(s) 76, 646, 900
MaeIII GTNAC 2 cut(s) 112, 1190
MalI GATC 2 cut(s) 227, 861
MboI GATC 2 cut(s) 225, 859
MboII GAAGA 7 cut(s) 46, 918, 950, 1048, 1142, 1204, 1273
MhlI GDGCHC 2 cut(s) 273, 556
MluCI AATT 6 cut(s) 138, 419, 455, 617, 1155, 1176
MluI ACGCGT 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 883
Mph1103I ATGCAT 1 cut(s) 109
MroXI GAANNNNTTC 1 cut(s) 1211
MseI TTAA 4 cut(s) 303, 417, 566, 873
MslI CAYNNNNRTG 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 731, 1218
MspI CCGG 1 cut(s) 670
MspR9I CCNGG 2 cut(s) 181, 443
Mva1269I GAATGC 3 cut(s) 107, 346, 1257
MvaI CCWGG 2 cut(s) 181, 443
MvnI CGCG 2 cut(s) 334, 1218
NdeI CATATG 1 cut(s) 471
NdeII GATC 2 cut(s) 225, 859
NlaIII CATG 6 cut(s) 107, 530, 947, 1022, 1046, 1302
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 3 cut(s) 107, 530, 1022
PaeI GCATGC 1 cut(s) 530
PceI AGGCCT 1 cut(s) 407
PciSI GCTCTTC 2 cut(s) 933, 1217
PctI GAATGC 3 cut(s) 107, 346, 1257
PdmI GAANNNNTTC 1 cut(s) 1211
PfeI GAWTC 4 cut(s) 752, 767, 887, 1070
Pfl23II CGTACG 1 cut(s) 257
PkrI GCNGC 3 cut(s) 525, 730, 733
PsiI TTATAA 3 cut(s) 579, 828, 855
Psp1406I AACGTT 2 cut(s) 646, 900
Psp6I CCWGG 2 cut(s) 179, 441
PspGI CCWGG 2 cut(s) 179, 441
PspLI CGTACG 1 cut(s) 257
PspPI GGNCC 1 cut(s) 439
PsrI GAACNNNNNNTAC 2 cut(s) 500, 532
PvuII CAGCTG 1 cut(s) 731
RsaI GTAC 3 cut(s) 259, 436, 1297
RsaNI GTAC 3 cut(s) 258, 435, 1296
RseI CAYNNNNRTG 1 cut(s) 531
SacII CCGCGG 1 cut(s) 1219
SapI GCTCTTC 2 cut(s) 933, 1217
SaqAI TTAA 4 cut(s) 303, 417, 566, 873
SatI GCNGC 3 cut(s) 524, 729, 732
Sau3AI GATC 2 cut(s) 225, 859
Sau96I GGNCC 1 cut(s) 439
ScrFI CCNGG 2 cut(s) 181, 443
SduI GDGCHC 2 cut(s) 273, 556
SexAI ACCWGGT 1 cut(s) 441
SfaNI GCATC 2 cut(s) 1131, 1213
Sfr303I CCGCGG 1 cut(s) 1219
SgrBI CCGCGG 1 cut(s) 1219
SinI GGWCC 1 cut(s) 439
SmiMI CAYNNNNRTG 1 cut(s) 531
SmlI CTYRAG 2 cut(s) 216, 695
SmoI CTYRAG 2 cut(s) 216, 695
SphI GCATGC 1 cut(s) 530
Sse9I AATT 6 cut(s) 138, 419, 455, 617, 1155, 1176
SseBI AGGCCT 1 cut(s) 407
SsiI CCGC 2 cut(s) 1216, 1218
SspI AATATT 1 cut(s) 301
SspMI CTAG 2 cut(s) 123, 914
StuI AGGCCT 1 cut(s) 407
StyD4I CCNGG 2 cut(s) 179, 441
StyI CCWWGG 2 cut(s) 402, 408
TaaI ACNGT 5 cut(s) 19, 96, 689, 809, 1190
TaiI ACGT 3 cut(s) 79, 649, 903
TaqI TCGA 2 cut(s) 292, 755
TasI AATT 6 cut(s) 138, 419, 455, 617, 1155, 1176
TatI WGTACW 1 cut(s) 1295
TfiI GAWTC 4 cut(s) 752, 767, 887, 1070
Tru1I TTAA 4 cut(s) 303, 417, 566, 873
Tru9I TTAA 4 cut(s) 303, 417, 566, 873
TscAI CASTG 2 cut(s) 24, 988
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 3 cut(s) 523, 728, 731
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 4 cut(s) 630, 1059, 1143, 1267
TspGWI ACGGA 2 cut(s) 245, 452
TspRI CASTG 2 cut(s) 24, 988
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 4 cut(s) 138, 419, 455, 1155
XceI RCATGY 3 cut(s) 107, 530, 1022
XmnI GAANNNNTTC 1 cut(s) 1211
XspI CTAG 2 cut(s) 123, 914
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.