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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 91 |
| AgsI | TTSAA | 3 cut(s) | 67, 124, 139 |
| AlwI | GGATC | 1 cut(s) | 91 |
| AsuII | TTCGAA | 1 cut(s) | 78 |
| Bpu14I | TTCGAA | 1 cut(s) | 78 |
| Bsp119I | TTCGAA | 1 cut(s) | 78 |
| Bsp143I | GATC | 1 cut(s) | 83 |
| BspPI | GGATC | 1 cut(s) | 91 |
| BspT104I | TTCGAA | 1 cut(s) | 78 |
| BssMI | GATC | 1 cut(s) | 83 |
| BstBI | TTCGAA | 1 cut(s) | 78 |
| BstKTI | GATC | 1 cut(s) | 86 |
| BstMBI | GATC | 1 cut(s) | 83 |
| BstX2I | RGATCY | 1 cut(s) | 83 |
| BstYI | RGATCY | 1 cut(s) | 83 |
| CviJI | RGCY | 1 cut(s) | 28 |
| CviKI_1 | RGCY | 1 cut(s) | 28 |
| DpnI | GATC | 1 cut(s) | 85 |
| DpnII | GATC | 1 cut(s) | 83 |
| FaiI | YATR | 1 cut(s) | 14 |
| FspEI | CC | 7 cut(s) | 3, 10, 66, 94, 113, 116, 159 |
| HinfI | GANTC | 1 cut(s) | 166 |
| Hpy188I | TCNGA | 1 cut(s) | 165 |
| Hpy188III | TCNNGA | 1 cut(s) | 146 |
| HpyAV | CCTTC | 1 cut(s) | 74 |
| HpyCH4V | TGCA | 1 cut(s) | 92 |
| Kzo9I | GATC | 1 cut(s) | 83 |
| LpnPI | CCDG | 3 cut(s) | 94, 116, 159 |
| MalI | GATC | 1 cut(s) | 85 |
| MboI | GATC | 1 cut(s) | 83 |
| MboII | GAAGA | 3 cut(s) | 62, 108, 152 |
| MflI | RGATCY | 1 cut(s) | 83 |
| MluCI | AATT | 3 cut(s) | 103, 124, 139 |
| NdeII | GATC | 1 cut(s) | 83 |
| NspV | TTCGAA | 1 cut(s) | 78 |
| PfeI | GAWTC | 1 cut(s) | 166 |
| PsuI | RGATCY | 1 cut(s) | 83 |
| Sau3AI | GATC | 1 cut(s) | 83 |
| SetI | ASST | 1 cut(s) | 113 |
| SfuI | TTCGAA | 1 cut(s) | 78 |
| SgeI | CNNG | 3 cut(s) | 121, 143, 158 |
| Sse9I | AATT | 3 cut(s) | 103, 124, 139 |
| TaqI | TCGA | 1 cut(s) | 78 |
| TasI | AATT | 3 cut(s) | 103, 124, 139 |
| TfiI | GAWTC | 1 cut(s) | 166 |
| TspDTI | ATGAA | 3 cut(s) | 24, 62, 108 |