RLG00000028775

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0000090
Physical Location & Seq
Reverse (-)
27798260 .. 27799007
748 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028775

Sequence Viewer

Length: 384 bp
ATGGACGAAATTGATGATGACAGCTACTCAAAAGCCTTGAAGCTCTTCCGCGGCGATGCTACTTGGAGAAAGCTGTTTGTGGGAATGCCTGAGAAGAGGAGAAAGAACTTCATCCTCAATCTTTTCTTTTCAGAGGGGAAGACACTCGCAAGGACAAGGGCCGTCGTGTCTGCTTTCACACTTTTCGAGACAACGATGAACTCGAGAGAGGGGAAGGTATCAATCCAGGAATTAAGAAGTTCTGTTATTGTGTTCTCAAAGGACTACGCTTCTTCGAAATGGTGCCTTGACGAGCATGTGATGATCCTTGAACGCAGGAGGGTCTCCTCCGATCATGTAGTTTTACCAGTTTTCGGACTACGGTTCTTCGAAATAGTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

128

Amino Acids

14.79

Weight (kDa)

9.41

Isoelectric Point (pI)

52.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 73 - 118 1.3e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000090)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18340 FvH4_2g08421 FvH4_3g05430 FvH4_3g26318 FvH4_3g43970 FvH4_5g24831 FvH4_6g20331 FvH4_6g23072 FvH4_7g02471 FvH4_7g02601 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790 FvH4_7g09790
malus_domestica MD09G1277700.v1.1
pyrus_communis pycom14g05970 pycom395g00100
rosa_chinensis RchiOBHm_Chr1g0329081 RchiOBHm_Chr1g0332631 RchiOBHm_Chr1g0341051 RchiOBHm_Chr1g0346821 RchiOBHm_Chr1g0346841 RchiOBHm_Chr1g0377301 RchiOBHm_Chr1g0378881 RchiOBHm_Chr1g0381361 RchiOBHm_Chr2g0094421 RchiOBHm_Chr2g0115791 RchiOBHm_Chr2g0131391 RchiOBHm_Chr3g0469161 RchiOBHm_Chr3g0477491 RchiOBHm_Chr4g0397121 RchiOBHm_Chr4g0403221 RchiOBHm_Chr4g0403811 RchiOBHm_Chr4g0418081 RchiOBHm_Chr4g0434301 RchiOBHm_Chr5g0004171 RchiOBHm_Chr5g0007651 RchiOBHm_Chr5g0025151 RchiOBHm_Chr5g0029021 RchiOBHm_Chr5g0036581 RchiOBHm_Chr5g0040531 RchiOBHm_Chr5g0041581 RchiOBHm_Chr5g0041591 RchiOBHm_Chr5g0055171 RchiOBHm_Chr5g0059931 RchiOBHm_Chr5g0070861 RchiOBHm_Chr5g0072491 RchiOBHm_Chr5g0073831 RchiOBHm_Chr5g0076701 RchiOBHm_Chr6g0264941 RchiOBHm_Chr6g0268711 RchiOBHm_Chr6g0272081 RchiOBHm_Chr7g0180801 RchiOBHm_Chr7g0206041 RchiOBHm_Chr7g0206051 RchiOBHm_Chr7g0206191 RchiOBHm_Chr7g0207401 RchiOBHm_Chr7g0208561 RchiOBHm_Chr7g0208571 RchiOBHm_Chr7g0232661 RchiOBHm_Chr7g0238901
rosa_laevigata RLG00000003152 RLG00000007513 RLG00000010392 RLG00000011803 RLG00000012560 RLG00000014764 RLG00000017712 RLG00000017809 RLG00000017860 RLG00000018875 RLG00000020741 RLG00000021294 RLG00000021737 RLG00000023224 RLG00000023340 RLG00000025142 RLG00000027945 RLG00000028772 RLG00000028775 RLG00000028776 RLG00000029536 RLG00000029993 RLG00000030256 RLG00000030594 RLG00000030697 RLG00000032707 RLG00000035344 RLG00000035458 RLG00000036306 RLG00000036953
rosa_multiflora Rmu_co8065394.1_g000001 Rmu_co8424083.1_g000001 Rmu_sc0000012.1_g000007 Rmu_sc0000073.1_g000003 Rmu_sc0000177.1_g000022 Rmu_sc0000356.1_g000009 Rmu_sc0000433.1_g000025 Rmu_sc0001229.1_g000008 Rmu_sc0001478.1_g000007 Rmu_sc0001552.1_g000046 Rmu_sc0001731.1_g000001 Rmu_sc0001731.1_g000004 Rmu_sc0001942.1_g000088 Rmu_sc0002530.1_g000034 Rmu_sc0002758.1_g000006 Rmu_sc0003033.1_g000034 Rmu_sc0003158.1_g000009 Rmu_sc0003193.1_g000001 Rmu_sc0003314.1_g000041 Rmu_sc0003756.1_g000007 Rmu_sc0003895.1_g000016 Rmu_sc0004149.1_g000005 Rmu_sc0004413.1_g000038 Rmu_sc0004509.1_g000025 Rmu_sc0005142.1_g000008 Rmu_sc0005291.1_g000005 Rmu_sc0005772.1_g000001 Rmu_sc0005962.1_g000002 Rmu_sc0006223.1_g000037 Rmu_sc0006898.1_g000018 Rmu_sc0007822.1_g000006 Rmu_sc0007822.1_g000007 Rmu_sc0015982.1_g000001 Rmu_sc0023577.1_g000001 Rmu_sc0026009.1_g000013 Rmu_ssc0000264.1_g000011 Rmu_ssc0000264.1_g000014 Rmu_ssc0000467.1_g000035
rosa_roxburghii Rroxscaffold_1G00003310 Rroxscaffold_1G00003440 Rroxscaffold_1G00025390 Rroxscaffold_1G00027650 Rroxscaffold_1G00035520 Rroxscaffold_1G00039720 Rroxscaffold_2G00096880 Rroxscaffold_2G00138110 Rroxscaffold_3G00223540 Rroxscaffold_3G00226400 Rroxscaffold_3G00249970 Rroxscaffold_3G00251230 Rroxscaffold_4G00308110 Rroxscaffold_4G00308440 Rroxscaffold_4G00308470 Rroxscaffold_4G00311680 Rroxscaffold_4G00311690 Rroxscaffold_4G00330900 Rroxscaffold_5G00342170 Rroxscaffold_5G00376290 Rroxscaffold_6G00392450 Rroxscaffold_6G00421400 Rroxscaffold_6G00430840 Rroxscaffold_7G00171590 Rroxscaffold_7G00178520 Rroxscaffold_7G00192720
rosa_rugosa Rorug01G0184200 Rorug02G0001800 Rorug02G0296300.1 Rorug02G0296400 Rorug02G0395800 Rorug03G0045600 Rorug03G0045700 Rorug03G0273100 Rorug04G0060900 Rorug04G0073900 Rorug04G0082100 Rorug04G0083700 Rorug04G0140600 Rorug04G0147600 Rorug04G0202000 Rorug04G0365300 Rorug05G0164600 Rorug05G0164700 Rorug05G0164800 Rorug05G0313400 Rorug05G0460400 Rorug05G0460500 Rorug06G0004700 Rorug06G0087800 Rorug07G0040400 Rorug07G0147000 Rorug07G0164100 Rorug07G0183900 Rorug07G0313600
rosa_samantha Rh1AG151200 Rh1AG190200 Rh1AG201300 Rh1AG409200 Rh1BG069600 Rh1BG167900 Rh1BG168100 Rh1BG221200 Rh1BG231700 Rh1BG279700 Rh1BG335200 Rh1CG100500 Rh1CG142400 Rh1CG175600 Rh1CG186200 Rh1CG348700 Rh1DG025200 Rh1DG040900 Rh1DG107900 Rh1DG108000 Rh1DG132100 Rh1DG198700 Rh1DG198900 Rh1DG248100 Rh1DG311000 Rh1DG366700 Rh2AG005300 Rh2AG262100 Rh2CG263100 Rh2CG313000 Rh2CG313100 Rh2CG335400 Rh2CG392500 Rh2CG576000 Rh2DG091000 Rh3AG032000 Rh3BG032400 Rh3CG031100 Rh3CG163600 Rh3CG311100 Rh3DG032000 Rh4AG091500 Rh4BG086500 Rh4BG111300 Rh4BG139900 Rh4CG015000 Rh4CG099800 Rh4DG210900 Rh4DG234600 Rh4DG282500 Rh5AG075100 Rh5AG228600 Rh5AG326300 Rh5AG464100 Rh5BG265900 Rh5BG284000 Rh5BG284900 Rh5CG337600 Rh5CG394700 Rh5DG206500 Rh5DG285000 Rh5DG293700 Rh5DG403900 Rh5DG469800 Rh6AG045500 Rh6AG242800 Rh6BG073100 Rh6BG202400 Rh6CG179100 Rh6CG202800 Rh6DG170800 Rh6DG194300 Rh6DG440100 Rh6DG445600 Rh6DG470700 Rh7BG262200 Rh7BG295500 Rh7BG295800 Rh7CG210600 Rh7CG421500 Rh7DG233400 Rh7DG233500 Rh7DG238100 Rh7DG238200 Rh7DG245500
rosa_wichuraiana Rw1G016920 Rw1G022060 Rw1G028030 Rw2G007760 Rw3G016140 Rw3G020210 Rw4G012320 Rw4G026900 Rw4G034750 Rw5G028220 Rw6G003840 Rw6G004130 Rw7G032360 Rw7G038920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 282
AccII CGCG 1 cut(s) 51
AciI CCGC 2 cut(s) 49, 51
AclWI GGATC 1 cut(s) 298
AfiI CCNNNNNNNGG 1 cut(s) 353
AgsI TTSAA 2 cut(s) 40, 311
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 3 cut(s) 24, 43, 73
AluI AGCT 3 cut(s) 24, 43, 73
Alw26I GTCTC 2 cut(s) 182, 328
AlwI GGATC 1 cut(s) 298
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 159
Asp700I GAANNNNTTC 1 cut(s) 44
AspS9I GGNCC 1 cut(s) 159
AsuII TTCGAA 2 cut(s) 275, 369
AvaI CYCGRG 1 cut(s) 202
BanI GGYRCC 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 146
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 227
BcoDI GTCTC 2 cut(s) 182, 328
BisI GCNGC 1 cut(s) 52
BlsI GCNGC 1 cut(s) 53
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 159
BmiI GGNNCC 1 cut(s) 284
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 1 cut(s) 46
BpiI GAAGAC 1 cut(s) 146
Bpu14I TTCGAA 2 cut(s) 275, 369
BsaI GGTCTC 1 cut(s) 328
BsaJI CCNNGG 1 cut(s) 49
Bsc4I CCNNNNNNNGG 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 347
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 1 cut(s) 111
BseLI CCNNNNNNNGG 1 cut(s) 353
BseMII CTCAG 1 cut(s) 81
BseNI ACTGG 1 cut(s) 347
BseRI GAGGAG 2 cut(s) 112, 316
Bsh1236I CGCG 1 cut(s) 51
BshFI GGCC 1 cut(s) 161
BshNI GGYRCC 1 cut(s) 282
BsiHKCI CYCGRG 1 cut(s) 202
BslI CCNNNNNNNGG 1 cut(s) 353
BsmAI GTCTC 2 cut(s) 182, 328
BsmI GAATGC 1 cut(s) 90
BsnI GGCC 1 cut(s) 161
Bso31I GGTCTC 1 cut(s) 328
BsoBI CYCGRG 1 cut(s) 202
Bsp119I TTCGAA 2 cut(s) 275, 369
Bsp143I GATC 2 cut(s) 303, 331
BspACI CCGC 2 cut(s) 49, 51
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 1 cut(s) 82
BspFNI CGCG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 284
BspPI GGATC 1 cut(s) 298
BspQI GCTCTTC 1 cut(s) 50
BspT104I TTCGAA 2 cut(s) 275, 369
BspT107I GGYRCC 1 cut(s) 282
BspTNI GGTCTC 1 cut(s) 328
BsrI ACTGG 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 2 cut(s) 303, 331
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 363
Bst6I CTCTTC 2 cut(s) 50, 89
BstBI TTCGAA 2 cut(s) 275, 369
BstDEI CTNAG 1 cut(s) 90
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 111
BstFNI CGCG 1 cut(s) 51
BstKTI GATC 2 cut(s) 306, 334
BstMAI GTCTC 2 cut(s) 182, 328
BstMBI GATC 2 cut(s) 303, 331
BstNI CCWGG 1 cut(s) 227
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 1 cut(s) 225
BstUI CGCG 1 cut(s) 51
BstV2I GAAGAC 1 cut(s) 146
BsuRI GGCC 1 cut(s) 161
BtgI CCRYGG 1 cut(s) 49
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 159
Cfr42I CCGCGG 1 cut(s) 52
CviAII CATG 2 cut(s) 296, 335
CviJI RGCY 5 cut(s) 24, 35, 43, 73, 161
CviKI_1 RGCY 5 cut(s) 24, 35, 43, 73, 161
DdeI CTNAG 1 cut(s) 90
DpnI GATC 2 cut(s) 305, 333
DpnII GATC 2 cut(s) 303, 331
Eam1104I CTCTTC 2 cut(s) 50, 89
EarI CTCTTC 2 cut(s) 50, 89
Eco31I GGTCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 225
FaeI CATG 2 cut(s) 299, 338
FaiI YATR 2 cut(s) 297, 336
FatI CATG 2 cut(s) 295, 334
Fnu4HI GCNGC 1 cut(s) 52
FokI GGATG 1 cut(s) 98
Fsp4HI GCNGC 1 cut(s) 52
GluI GCNGC 1 cut(s) 52
HaeIII GGCC 1 cut(s) 161
Hin1II CATG 2 cut(s) 299, 338
Hpy188I TCNGA 3 cut(s) 133, 331, 356
Hpy188III TCNNGA 2 cut(s) 187, 204
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 208
HpyCH4III ACNGT 1 cut(s) 363
HpyF3I CTNAG 1 cut(s) 90
Hsp92II CATG 2 cut(s) 299, 338
KspI CCGCGG 1 cut(s) 52
Kzo9I GATC 2 cut(s) 303, 331
LguI GCTCTTC 1 cut(s) 50
LpnPI CCDG 5 cut(s) 102, 212, 239, 301, 360
LweI GCATC 1 cut(s) 46
MalI GATC 2 cut(s) 305, 333
MboI GATC 2 cut(s) 303, 331
MboII GAAGA 5 cut(s) 37, 106, 151, 264, 358
MluCI AATT 2 cut(s) 9, 230
MnlI CCTC 6 cut(s) 90, 125, 127, 202, 312, 337
MroXI GAANNNNTTC 1 cut(s) 44
MseI TTAA 1 cut(s) 233
MspA1I CMGCKG 1 cut(s) 51
MspR9I CCNGG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 90
MvaI CCWGG 1 cut(s) 227
MvnI CGCG 1 cut(s) 51
NdeII GATC 2 cut(s) 303, 331
NlaIII CATG 2 cut(s) 299, 338
NlaIV GGNNCC 1 cut(s) 284
NspI RCATGY 1 cut(s) 299
NspV TTCGAA 2 cut(s) 275, 369
PaeR7I CTCGAG 1 cut(s) 202
PciSI GCTCTTC 1 cut(s) 50
PcsI WCGNNNNNNNCGW 1 cut(s) 200
PctI GAATGC 1 cut(s) 90
PdmI GAANNNNTTC 1 cut(s) 44
PfoI TCCNGGA 1 cut(s) 225
PkrI GCNGC 1 cut(s) 53
Psp6I CCWGG 1 cut(s) 225
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 284
PspPI GGNCC 1 cut(s) 159
SacII CCGCGG 1 cut(s) 52
SapI GCTCTTC 1 cut(s) 50
SaqAI TTAA 1 cut(s) 233
SatI GCNGC 1 cut(s) 52
Sau3AI GATC 2 cut(s) 303, 331
Sau96I GGNCC 1 cut(s) 159
ScrFI CCNGG 1 cut(s) 227
SetI ASST 4 cut(s) 26, 45, 75, 219
SfaNI GCATC 1 cut(s) 46
Sfr274I CTCGAG 1 cut(s) 202
Sfr303I CCGCGG 1 cut(s) 52
SfuI TTCGAA 2 cut(s) 275, 369
SgrBI CCGCGG 1 cut(s) 52
SlaI CTCGAG 1 cut(s) 202
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
Sse9I AATT 2 cut(s) 9, 230
SsiI CCGC 2 cut(s) 49, 51
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 363
TaqI TCGA 4 cut(s) 186, 203, 275, 369
TasI AATT 2 cut(s) 9, 230
TauI GCSGC 1 cut(s) 54
Tru1I TTAA 1 cut(s) 233
Tru9I TTAA 1 cut(s) 233
TspDTI ATGAA 2 cut(s) 100, 212
XceI RCATGY 1 cut(s) 299
XhoI CTCGAG 1 cut(s) 202
XmnI GAANNNNTTC 1 cut(s) 44
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.