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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 1 cut(s) | 157 |
| AluBI | AGCT | 1 cut(s) | 86 |
| AluI | AGCT | 1 cut(s) | 86 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 172 |
| BpuEI | CTTGAG | 1 cut(s) | 147 |
| Bse3DI | GCAATG | 1 cut(s) | 7 |
| BseMI | GCAATG | 1 cut(s) | 7 |
| BseMII | CTCAG | 2 cut(s) | 69, 142 |
| BslFI | GGGAC | 1 cut(s) | 8 |
| BsmFI | GGGAC | 1 cut(s) | 8 |
| Bsp143I | GATC | 2 cut(s) | 3, 97 |
| BspCNI | CTCAG | 2 cut(s) | 70, 141 |
| BsrDI | GCAATG | 1 cut(s) | 7 |
| BssMI | GATC | 2 cut(s) | 3, 97 |
| BstDEI | CTNAG | 2 cut(s) | 78, 128 |
| BstKTI | GATC | 2 cut(s) | 6, 100 |
| BstMBI | GATC | 2 cut(s) | 3, 97 |
| BstNSI | RCATGY | 1 cut(s) | 127 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 14 |
| CviAII | CATG | 2 cut(s) | 60, 124 |
| CviJI | RGCY | 2 cut(s) | 86, 161 |
| CviKI_1 | RGCY | 2 cut(s) | 86, 161 |
| DdeI | CTNAG | 2 cut(s) | 78, 128 |
| DpnI | GATC | 2 cut(s) | 5, 99 |
| DpnII | GATC | 2 cut(s) | 3, 97 |
| FaeI | CATG | 2 cut(s) | 63, 127 |
| FaiI | YATR | 5 cut(s) | 32, 44, 61, 125, 134 |
| FaqI | GGGAC | 1 cut(s) | 8 |
| FatI | CATG | 2 cut(s) | 59, 123 |
| Hin1II | CATG | 2 cut(s) | 63, 127 |
| Hpy188I | TCNGA | 1 cut(s) | 79 |
| HpyAV | CCTTC | 2 cut(s) | 130, 151 |
| HpyCH4IV | ACGT | 1 cut(s) | 34 |
| HpyCH4V | TGCA | 2 cut(s) | 12, 59 |
| HpyF3I | CTNAG | 2 cut(s) | 78, 128 |
| HpySE526I | ACGT | 1 cut(s) | 34 |
| Hsp92II | CATG | 2 cut(s) | 63, 127 |
| Kzo9I | GATC | 2 cut(s) | 3, 97 |
| MaeII | ACGT | 1 cut(s) | 34 |
| MalI | GATC | 2 cut(s) | 5, 99 |
| MboI | GATC | 2 cut(s) | 3, 97 |
| MluCI | AATT | 2 cut(s) | 73, 89 |
| MnlI | CCTC | 3 cut(s) | 36, 73, 174 |
| MseI | TTAA | 1 cut(s) | 120 |
| NdeII | GATC | 2 cut(s) | 3, 97 |
| NlaIII | CATG | 2 cut(s) | 63, 127 |
| NspI | RCATGY | 1 cut(s) | 127 |
| SaqAI | TTAA | 1 cut(s) | 120 |
| Sau3AI | GATC | 2 cut(s) | 3, 97 |
| SetI | ASST | 4 cut(s) | 37, 84, 88, 119 |
| SgeI | CNNG | 4 cut(s) | 72, 107, 136, 176 |
| SmlI | CTYRAG | 1 cut(s) | 162 |
| SmoI | CTYRAG | 1 cut(s) | 162 |
| Sse9I | AATT | 2 cut(s) | 73, 89 |
| TaiI | ACGT | 1 cut(s) | 37 |
| TaqI | TCGA | 1 cut(s) | 105 |
| TasI | AATT | 2 cut(s) | 73, 89 |
| Tru1I | TTAA | 1 cut(s) | 120 |
| Tru9I | TTAA | 1 cut(s) | 120 |
| TspDTI | ATGAA | 1 cut(s) | 149 |
| XceI | RCATGY | 1 cut(s) | 127 |