B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 881 |
| Acc36I | ACCTGC | 1 cut(s) | 881 |
| Acc65I | GGTACC | 1 cut(s) | 563 |
| AccB1I | GGYRCC | 1 cut(s) | 563 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 521 |
| AccI | GTMKAC | 1 cut(s) | 545 |
| AciI | CCGC | 2 cut(s) | 129, 324 |
| AclWI | GGATC | 1 cut(s) | 240 |
| AcoI | YGGCCR | 2 cut(s) | 126, 335 |
| AcsI | RAATTY | 1 cut(s) | 647 |
| AcuI | CTGAAG | 2 cut(s) | 233, 765 |
| AfaI | GTAC | 3 cut(s) | 477, 565, 714 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 41, 323, 521 |
| AflII | CTTAAG | 1 cut(s) | 308 |
| AgsI | TTSAA | 7 cut(s) | 60, 104, 112, 487, 558, 646, 844 |
| AjnI | CCWGG | 1 cut(s) | 40 |
| AluBI | AGCT | 6 cut(s) | 204, 266, 447, 530, 635, 953 |
| AluI | AGCT | 6 cut(s) | 204, 266, 447, 530, 635, 953 |
| Alw26I | GTCTC | 2 cut(s) | 591, 759 |
| AlwI | GGATC | 1 cut(s) | 240 |
| Ama87I | CYCGRG | 1 cut(s) | 948 |
| AoxI | GGCC | 3 cut(s) | 43, 126, 335 |
| ApoI | RAATTY | 1 cut(s) | 647 |
| Asp718I | GGTACC | 1 cut(s) | 563 |
| AsuHPI | GGTGA | 3 cut(s) | 718, 797, 905 |
| AvaI | CYCGRG | 1 cut(s) | 948 |
| BalI | TGGCCA | 1 cut(s) | 337 |
| BanI | GGYRCC | 1 cut(s) | 563 |
| BccI | CCATC | 4 cut(s) | 118, 236, 384, 737 |
| BciT130I | CCWGG | 1 cut(s) | 42 |
| BcoDI | GTCTC | 2 cut(s) | 591, 759 |
| BfaI | CTAG | 1 cut(s) | 663 |
| BfrI | CTTAAG | 1 cut(s) | 308 |
| BfuAI | ACCTGC | 1 cut(s) | 881 |
| BisI | GCNGC | 1 cut(s) | 129 |
| BlsI | GCNGC | 1 cut(s) | 130 |
| Bme1390I | CCNGG | 1 cut(s) | 42 |
| BmeT110I | CYCGRG | 1 cut(s) | 948 |
| BmiI | GGNNCC | 2 cut(s) | 315, 565 |
| BmrFI | CCNGG | 1 cut(s) | 42 |
| BmrI | ACTGGG | 1 cut(s) | 182 |
| BmsI | GCATC | 2 cut(s) | 527, 590 |
| BmuI | ACTGGG | 1 cut(s) | 182 |
| BpuEI | CTTGAG | 1 cut(s) | 727 |
| BsaI | GGTCTC | 2 cut(s) | 591, 759 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 398, 428 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 41, 323, 521 |
| Bse1I | ACTGG | 2 cut(s) | 188, 338 |
| Bse3DI | GCAATG | 1 cut(s) | 138 |
| BseBI | CCWGG | 1 cut(s) | 42 |
| BseGI | GGATG | 2 cut(s) | 395, 542 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 41, 323, 521 |
| BseMI | GCAATG | 1 cut(s) | 138 |
| BseNI | ACTGG | 2 cut(s) | 188, 338 |
| BseRI | GAGGAG | 2 cut(s) | 34, 540 |
| BshFI | GGCC | 3 cut(s) | 45, 128, 337 |
| BshNI | GGYRCC | 1 cut(s) | 563 |
| BsiHKCI | CYCGRG | 1 cut(s) | 948 |
| BslFI | GGGAC | 1 cut(s) | 668 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 41, 323, 521 |
| BsmAI | GTCTC | 2 cut(s) | 591, 759 |
| BsmFI | GGGAC | 1 cut(s) | 668 |
| BsnI | GGCC | 3 cut(s) | 45, 128, 337 |
| Bso31I | GGTCTC | 2 cut(s) | 591, 759 |
| BsoBI | CYCGRG | 1 cut(s) | 948 |
| Bsp143I | GATC | 2 cut(s) | 118, 232 |
| BspACI | CCGC | 2 cut(s) | 129, 324 |
| BspANI | GGCC | 3 cut(s) | 45, 128, 337 |
| BspLI | GGNNCC | 2 cut(s) | 315, 565 |
| BspMI | ACCTGC | 1 cut(s) | 881 |
| BspPI | GGATC | 1 cut(s) | 240 |
| BspT107I | GGYRCC | 1 cut(s) | 563 |
| BspTI | CTTAAG | 1 cut(s) | 308 |
| BspTNI | GGTCTC | 2 cut(s) | 591, 759 |
| BsrDI | GCAATG | 1 cut(s) | 138 |
| BsrI | ACTGG | 2 cut(s) | 188, 338 |
| BssMI | GATC | 2 cut(s) | 118, 232 |
| Bst2UI | CCWGG | 1 cut(s) | 42 |
| Bst4CI | ACNGT | 2 cut(s) | 766, 802 |
| Bst6I | CTCTTC | 2 cut(s) | 11, 166 |
| BstAFI | CTTAAG | 1 cut(s) | 308 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 587 |
| BstC8I | GCNNGC | 2 cut(s) | 264, 592 |
| BstEII | GGTNACC | 1 cut(s) | 509 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 39 |
| BstF5I | GGATG | 2 cut(s) | 395, 542 |
| BstKTI | GATC | 2 cut(s) | 121, 235 |
| BstMAI | GTCTC | 2 cut(s) | 591, 759 |
| BstMBI | GATC | 2 cut(s) | 118, 232 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 453, 587 |
| BstNI | CCWGG | 1 cut(s) | 42 |
| BstNSI | RCATGY | 2 cut(s) | 182, 544 |
| BstPI | GGTNACC | 1 cut(s) | 509 |
| BstSCI | CCNGG | 1 cut(s) | 40 |
| BstX2I | RGATCY | 1 cut(s) | 232 |
| BstYI | RGATCY | 1 cut(s) | 232 |
| BsuRI | GGCC | 3 cut(s) | 45, 128, 337 |
| BtsCI | GGATG | 2 cut(s) | 395, 542 |
| BtsIMutI | CAGTG | 2 cut(s) | 195, 812 |
| BveI | ACCTGC | 1 cut(s) | 881 |
| Cac8I | GCNNGC | 2 cut(s) | 264, 592 |
| Csp6I | GTAC | 3 cut(s) | 476, 564, 713 |
| CviAII | CATG | 3 cut(s) | 179, 333, 541 |
| CviQI | GTAC | 3 cut(s) | 476, 564, 713 |
| DpnI | GATC | 2 cut(s) | 120, 234 |
| DpnII | GATC | 2 cut(s) | 118, 232 |
| EaeI | YGGCCR | 2 cut(s) | 126, 335 |
| Eam1104I | CTCTTC | 2 cut(s) | 11, 166 |
| EarI | CTCTTC | 2 cut(s) | 11, 166 |
| Eco31I | GGTCTC | 2 cut(s) | 591, 759 |
| Eco32I | GATATC | 1 cut(s) | 878 |
| Eco57I | CTGAAG | 2 cut(s) | 233, 765 |
| Eco88I | CYCGRG | 1 cut(s) | 948 |
| Eco91I | GGTNACC | 1 cut(s) | 509 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 39 |
| EcoO65I | GGTNACC | 1 cut(s) | 509 |
| EcoRII | CCWGG | 1 cut(s) | 40 |
| EcoRV | GATATC | 1 cut(s) | 878 |
| EcoT22I | ATGCAT | 1 cut(s) | 542 |
| FaeI | CATG | 3 cut(s) | 182, 336, 544 |
| FaqI | GGGAC | 1 cut(s) | 668 |
| FatI | CATG | 3 cut(s) | 178, 332, 540 |
| FblI | GTMKAC | 1 cut(s) | 545 |
| Fnu4HI | GCNGC | 1 cut(s) | 129 |
| FokI | GGATG | 2 cut(s) | 402, 549 |
| Fsp4HI | GCNGC | 1 cut(s) | 129 |
| FspBI | CTAG | 1 cut(s) | 663 |
| GluI | GCNGC | 1 cut(s) | 129 |
| HaeIII | GGCC | 3 cut(s) | 45, 128, 337 |
| Hin1II | CATG | 3 cut(s) | 182, 336, 544 |
| HincII | GTYRAC | 1 cut(s) | 940 |
| HindII | GTYRAC | 1 cut(s) | 940 |
| HindIII | AAGCTT | 1 cut(s) | 202 |
| HinfI | GANTC | 7 cut(s) | 4, 194, 296, 358, 372, 399, 840 |
| HphI | GGTGA | 3 cut(s) | 718, 797, 905 |
| Hpy166II | GTNNAC | 4 cut(s) | 193, 478, 546, 940 |
| Hpy188I | TCNGA | 3 cut(s) | 67, 123, 156 |
| Hpy188III | TCNNGA | 3 cut(s) | 257, 706, 948 |
| Hpy8I | GTNNAC | 4 cut(s) | 193, 478, 546, 940 |
| HpyAV | CCTTC | 5 cut(s) | 193, 241, 446, 726, 865 |
| HpyCH4III | ACNGT | 2 cut(s) | 766, 802 |
| HpyCH4IV | ACGT | 2 cut(s) | 619, 925 |
| HpyCH4V | TGCA | 4 cut(s) | 492, 540, 581, 836 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 453, 587 |
| HpySE526I | ACGT | 2 cut(s) | 619, 925 |
| Hsp92II | CATG | 3 cut(s) | 182, 336, 544 |
| KpnI | GGTACC | 1 cut(s) | 567 |
| Kzo9I | GATC | 2 cut(s) | 118, 232 |
| LmnI | GCTCC | 3 cut(s) | 156, 527, 632 |
| LweI | GCATC | 2 cut(s) | 527, 590 |
| MaeI | CTAG | 1 cut(s) | 663 |
| MaeII | ACGT | 2 cut(s) | 619, 925 |
| MaeIII | GTNAC | 9 cut(s) | 28, 85, 135, 268, 378, 509, 620, 673, 766 |
| MalI | GATC | 2 cut(s) | 120, 234 |
| MboI | GATC | 2 cut(s) | 118, 232 |
| MboII | GAAGA | 3 cut(s) | 28, 183, 226 |
| MfeI | CAATTG | 2 cut(s) | 319, 881 |
| MflI | RGATCY | 1 cut(s) | 232 |
| MlsI | TGGCCA | 1 cut(s) | 337 |
| MluCI | AATT | 8 cut(s) | 79, 283, 319, 516, 553, 640, 647, 881 |
| MluNI | TGGCCA | 1 cut(s) | 337 |
| MlyI | GAGTC | 2 cut(s) | 188, 290 |
| MmeI | TCCRAC | 1 cut(s) | 836 |
| MnlI | CCTC | 7 cut(s) | 12, 160, 167, 518, 577, 586, 854 |
| Mox20I | TGGCCA | 1 cut(s) | 337 |
| Mph1103I | ATGCAT | 1 cut(s) | 542 |
| MscI | TGGCCA | 1 cut(s) | 337 |
| MseI | TTAA | 2 cut(s) | 12, 309 |
| MslI | CAYNNNNRTG | 1 cut(s) | 741 |
| Msp20I | TGGCCA | 1 cut(s) | 337 |
| MspA1I | CMGCKG | 1 cut(s) | 266 |
| MspCI | CTTAAG | 1 cut(s) | 308 |
| MspR9I | CCNGG | 1 cut(s) | 42 |
| MunI | CAATTG | 2 cut(s) | 319, 881 |
| MvaI | CCWGG | 1 cut(s) | 42 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 453, 587 |
| NdeII | GATC | 2 cut(s) | 118, 232 |
| NlaIII | CATG | 3 cut(s) | 182, 336, 544 |
| NlaIV | GGNNCC | 2 cut(s) | 315, 565 |
| NmuCI | GTSAC | 5 cut(s) | 135, 268, 620, 673, 766 |
| NsiI | ATGCAT | 1 cut(s) | 542 |
| NspI | RCATGY | 2 cut(s) | 182, 544 |
| PaqCI | CACCTGC | 1 cut(s) | 881 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 367 |
| PfeI | GAWTC | 4 cut(s) | 358, 372, 399, 840 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 521 |
| PkrI | GCNGC | 1 cut(s) | 130 |
| PleI | GAGTC | 2 cut(s) | 188, 290 |
| PpsI | GAGTC | 2 cut(s) | 188, 290 |
| Psp6I | CCWGG | 1 cut(s) | 40 |
| PspEI | GGTNACC | 1 cut(s) | 509 |
| PspGI | CCWGG | 1 cut(s) | 40 |
| PspN4I | GGNNCC | 2 cut(s) | 315, 565 |
| PsuI | RGATCY | 1 cut(s) | 232 |
| PvuII | CAGCTG | 1 cut(s) | 266 |
| RsaI | GTAC | 3 cut(s) | 477, 565, 714 |
| RsaNI | GTAC | 3 cut(s) | 476, 564, 713 |
| RseI | CAYNNNNRTG | 1 cut(s) | 741 |
| SaqAI | TTAA | 2 cut(s) | 12, 309 |
| SatI | GCNGC | 1 cut(s) | 129 |
| Sau3AI | GATC | 2 cut(s) | 118, 232 |
| SchI | GAGTC | 2 cut(s) | 188, 290 |
| ScrFI | CCNGG | 1 cut(s) | 42 |
| SfaNI | GCATC | 2 cut(s) | 527, 590 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 741 |
| SmlI | CTYRAG | 2 cut(s) | 308, 706 |
| SmoI | CTYRAG | 2 cut(s) | 308, 706 |
| Sse9I | AATT | 8 cut(s) | 79, 283, 319, 516, 553, 640, 647, 881 |
| SsiI | CCGC | 2 cut(s) | 129, 324 |
| SspMI | CTAG | 1 cut(s) | 663 |
| StyD4I | CCNGG | 1 cut(s) | 40 |
| TaaI | ACNGT | 2 cut(s) | 766, 802 |
| TaiI | ACGT | 2 cut(s) | 622, 928 |
| TaqI | TCGA | 1 cut(s) | 732 |
| TasI | AATT | 8 cut(s) | 79, 283, 319, 516, 553, 640, 647, 881 |
| TatI | WGTACW | 1 cut(s) | 475 |
| TauI | GCSGC | 1 cut(s) | 131 |
| TfiI | GAWTC | 4 cut(s) | 358, 372, 399, 840 |
| Tru1I | TTAA | 2 cut(s) | 12, 309 |
| Tru9I | TTAA | 2 cut(s) | 12, 309 |
| TscAI | CASTG | 2 cut(s) | 195, 812 |
| TseFI | GTSAC | 5 cut(s) | 135, 268, 620, 673, 766 |
| Tsp45I | GTSAC | 5 cut(s) | 135, 268, 620, 673, 766 |
| TspDTI | ATGAA | 2 cut(s) | 424, 465 |
| TspRI | CASTG | 2 cut(s) | 195, 812 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 521 |
| Vha464I | CTTAAG | 1 cut(s) | 308 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 39 |
| XapI | RAATTY | 1 cut(s) | 647 |
| XceI | RCATGY | 2 cut(s) | 182, 544 |
| XmiI | GTMKAC | 1 cut(s) | 545 |
| XspI | CTAG | 1 cut(s) | 663 |
| Zsp2I | ATGCAT | 1 cut(s) | 542 |