B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 761 |
| Acc36I | ACCTGC | 1 cut(s) | 761 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 398 |
| AccI | GTMKAC | 3 cut(s) | 425, 819, 825 |
| AciI | CCGC | 1 cut(s) | 201 |
| AclWI | GGATC | 3 cut(s) | 41, 117, 560 |
| AcoI | YGGCCR | 2 cut(s) | 3, 212 |
| AcuI | CTGAAG | 1 cut(s) | 645 |
| AfaI | GTAC | 3 cut(s) | 354, 594, 691 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 200, 398 |
| AgsI | TTSAA | 2 cut(s) | 91, 724 |
| AjnI | CCWGG | 1 cut(s) | 409 |
| AluBI | AGCT | 8 cut(s) | 81, 143, 324, 447, 515, 581, 811, 833 |
| AluI | AGCT | 8 cut(s) | 81, 143, 324, 447, 515, 581, 811, 833 |
| Alw26I | GTCTC | 1 cut(s) | 471 |
| AlwI | GGATC | 3 cut(s) | 41, 117, 560 |
| AoxI | GGCC | 2 cut(s) | 3, 212 |
| ApeKI | GCWGC | 1 cut(s) | 728 |
| AspS9I | GGNCC | 1 cut(s) | 407 |
| AsuC2I | CCSGG | 1 cut(s) | 65 |
| AsuHPI | GGTGA | 3 cut(s) | 598, 677, 785 |
| AvaII | GGWCC | 1 cut(s) | 407 |
| BalI | TGGCCA | 2 cut(s) | 5, 214 |
| BbvI | GCAGC | 1 cut(s) | 715 |
| BccI | CCATC | 3 cut(s) | 113, 261, 617 |
| BciT130I | CCWGG | 1 cut(s) | 411 |
| BcnI | CCSGG | 1 cut(s) | 65 |
| BcoDI | GTCTC | 1 cut(s) | 471 |
| BfuAI | ACCTGC | 1 cut(s) | 761 |
| BisI | GCNGC | 1 cut(s) | 729 |
| BlsI | GCNGC | 1 cut(s) | 730 |
| Bme1390I | CCNGG | 2 cut(s) | 65, 411 |
| Bme18I | GGWCC | 1 cut(s) | 407 |
| BmgT120I | GGNCC | 1 cut(s) | 407 |
| BmiI | GGNNCC | 1 cut(s) | 192 |
| BmrFI | CCNGG | 2 cut(s) | 65, 411 |
| BmsI | GCATC | 2 cut(s) | 407, 470 |
| BpuEI | CTTGAG | 1 cut(s) | 170 |
| BpuMI | CCSGG | 1 cut(s) | 65 |
| BsaAI | YACGTR | 1 cut(s) | 823 |
| BsaI | GGTCTC | 1 cut(s) | 471 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 275, 305 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 200, 398 |
| Bse1I | ACTGG | 2 cut(s) | 215, 790 |
| BseBI | CCWGG | 1 cut(s) | 411 |
| BseGI | GGATG | 1 cut(s) | 272 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 200, 398 |
| BseNI | ACTGG | 2 cut(s) | 215, 790 |
| BseRI | GAGGAG | 1 cut(s) | 417 |
| BseXI | GCAGC | 1 cut(s) | 715 |
| BsgI | GTGCAG | 1 cut(s) | 770 |
| BshFI | GGCC | 2 cut(s) | 5, 214 |
| BsiSI | CCGG | 1 cut(s) | 65 |
| BslFI | GGGAC | 1 cut(s) | 548 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 200, 398 |
| BsmAI | GTCTC | 1 cut(s) | 471 |
| BsmFI | GGGAC | 1 cut(s) | 548 |
| BsnI | GGCC | 2 cut(s) | 5, 214 |
| Bso31I | GGTCTC | 1 cut(s) | 471 |
| Bsp1407I | TGTACA | 1 cut(s) | 689 |
| Bsp143I | GATC | 3 cut(s) | 33, 109, 552 |
| BspACI | CCGC | 1 cut(s) | 201 |
| BspANI | GGCC | 2 cut(s) | 5, 214 |
| BspLI | GGNNCC | 1 cut(s) | 192 |
| BspMI | ACCTGC | 1 cut(s) | 761 |
| BspPI | GGATC | 3 cut(s) | 41, 117, 560 |
| BspTNI | GGTCTC | 1 cut(s) | 471 |
| BsrGI | TGTACA | 1 cut(s) | 689 |
| BsrI | ACTGG | 2 cut(s) | 215, 790 |
| BssMI | GATC | 3 cut(s) | 33, 109, 552 |
| BssNAI | GTATAC | 1 cut(s) | 826 |
| Bst1107I | GTATAC | 1 cut(s) | 826 |
| Bst2UI | CCWGG | 1 cut(s) | 411 |
| Bst4CI | ACNGT | 3 cut(s) | 276, 368, 682 |
| Bst6I | CTCTTC | 1 cut(s) | 43 |
| BstAUI | TGTACA | 1 cut(s) | 689 |
| BstBAI | YACGTR | 1 cut(s) | 823 |
| BstC8I | GCNNGC | 1 cut(s) | 141 |
| BstF5I | GGATG | 1 cut(s) | 272 |
| BstKTI | GATC | 3 cut(s) | 36, 112, 555 |
| BstMAI | GTCTC | 1 cut(s) | 471 |
| BstMBI | GATC | 3 cut(s) | 33, 109, 552 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 330 |
| BstNI | CCWGG | 1 cut(s) | 411 |
| BstNSI | RCATGY | 1 cut(s) | 59 |
| BstSCI | CCNGG | 2 cut(s) | 63, 409 |
| BstSNI | TACGTA | 1 cut(s) | 823 |
| BstV1I | GCAGC | 1 cut(s) | 715 |
| BstX2I | RGATCY | 1 cut(s) | 109 |
| BstYI | RGATCY | 1 cut(s) | 109 |
| BstZ17I | GTATAC | 1 cut(s) | 826 |
| BsuRI | GGCC | 2 cut(s) | 5, 214 |
| BtsCI | GGATG | 1 cut(s) | 272 |
| BtsIMutI | CAGTG | 1 cut(s) | 364 |
| BveI | ACCTGC | 1 cut(s) | 761 |
| Cac8I | GCNNGC | 1 cut(s) | 141 |
| Cfr13I | GGNCC | 1 cut(s) | 407 |
| CsiI | ACCWGGT | 1 cut(s) | 409 |
| Csp6I | GTAC | 3 cut(s) | 353, 593, 690 |
| CviAII | CATG | 2 cut(s) | 56, 210 |
| CviQI | GTAC | 3 cut(s) | 353, 593, 690 |
| DpnI | GATC | 3 cut(s) | 35, 111, 554 |
| DpnII | GATC | 3 cut(s) | 33, 109, 552 |
| DraI | TTTAAA | 1 cut(s) | 438 |
| EaeI | YGGCCR | 2 cut(s) | 3, 212 |
| Eam1104I | CTCTTC | 1 cut(s) | 43 |
| EarI | CTCTTC | 1 cut(s) | 43 |
| Eco105I | TACGTA | 1 cut(s) | 823 |
| Eco31I | GGTCTC | 1 cut(s) | 471 |
| Eco47I | GGWCC | 1 cut(s) | 407 |
| Eco57I | CTGAAG | 1 cut(s) | 645 |
| EcoO109I | RGGNCCY | 1 cut(s) | 407 |
| EcoRII | CCWGG | 1 cut(s) | 409 |
| FaeI | CATG | 2 cut(s) | 59, 213 |
| FaqI | GGGAC | 1 cut(s) | 548 |
| FatI | CATG | 2 cut(s) | 55, 209 |
| FblI | GTMKAC | 3 cut(s) | 425, 819, 825 |
| Fnu4HI | GCNGC | 1 cut(s) | 729 |
| FokI | GGATG | 1 cut(s) | 279 |
| Fsp4HI | GCNGC | 1 cut(s) | 729 |
| GluI | GCNGC | 1 cut(s) | 729 |
| HaeIII | GGCC | 2 cut(s) | 5, 214 |
| HapII | CCGG | 1 cut(s) | 65 |
| Hin1II | CATG | 2 cut(s) | 59, 213 |
| HindIII | AAGCTT | 1 cut(s) | 79 |
| HinfI | GANTC | 6 cut(s) | 71, 173, 235, 249, 566, 720 |
| HpaII | CCGG | 1 cut(s) | 65 |
| HphI | GGTGA | 3 cut(s) | 598, 677, 785 |
| Hpy166II | GTNNAC | 6 cut(s) | 70, 279, 355, 426, 820, 826 |
| Hpy188I | TCNGA | 3 cut(s) | 33, 552, 643 |
| Hpy188III | TCNNGA | 2 cut(s) | 37, 134 |
| Hpy8I | GTNNAC | 6 cut(s) | 70, 279, 355, 426, 820, 826 |
| HpyAV | CCTTC | 4 cut(s) | 70, 118, 323, 606 |
| HpyCH4III | ACNGT | 3 cut(s) | 276, 368, 682 |
| HpyCH4IV | ACGT | 2 cut(s) | 805, 822 |
| HpyCH4V | TGCA | 3 cut(s) | 420, 461, 751 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 330 |
| HpySE526I | ACGT | 2 cut(s) | 805, 822 |
| Hsp92II | CATG | 2 cut(s) | 59, 213 |
| Kzo9I | GATC | 3 cut(s) | 33, 109, 552 |
| LmnI | GCTCC | 2 cut(s) | 452, 512 |
| Lsp1109I | GCAGC | 1 cut(s) | 715 |
| LweI | GCATC | 2 cut(s) | 407, 470 |
| MabI | ACCWGGT | 1 cut(s) | 409 |
| MaeII | ACGT | 2 cut(s) | 805, 822 |
| MaeIII | GTNAC | 5 cut(s) | 12, 97, 255, 368, 646 |
| MalI | GATC | 3 cut(s) | 35, 111, 554 |
| MboI | GATC | 3 cut(s) | 33, 109, 552 |
| MboII | GAAGA | 3 cut(s) | 60, 103, 729 |
| MfeI | CAATTG | 2 cut(s) | 196, 761 |
| MflI | RGATCY | 1 cut(s) | 109 |
| MlsI | TGGCCA | 2 cut(s) | 5, 214 |
| MluCI | AATT | 6 cut(s) | 160, 196, 393, 433, 520, 761 |
| MluNI | TGGCCA | 2 cut(s) | 5, 214 |
| MlyI | GAGTC | 2 cut(s) | 65, 167 |
| MmeI | TCCRAC | 1 cut(s) | 716 |
| MnlI | CCTC | 7 cut(s) | 37, 44, 141, 194, 395, 398, 466 |
| Mox20I | TGGCCA | 2 cut(s) | 5, 214 |
| MscI | TGGCCA | 2 cut(s) | 5, 214 |
| MseI | TTAA | 1 cut(s) | 437 |
| MslI | CAYNNNNRTG | 1 cut(s) | 621 |
| Msp20I | TGGCCA | 2 cut(s) | 5, 214 |
| MspA1I | CMGCKG | 1 cut(s) | 143 |
| MspI | CCGG | 1 cut(s) | 65 |
| MspR9I | CCNGG | 2 cut(s) | 65, 411 |
| MunI | CAATTG | 2 cut(s) | 196, 761 |
| MvaI | CCWGG | 1 cut(s) | 411 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 330 |
| NciI | CCSGG | 1 cut(s) | 65 |
| NdeII | GATC | 3 cut(s) | 33, 109, 552 |
| NlaIII | CATG | 2 cut(s) | 59, 213 |
| NlaIV | GGNNCC | 1 cut(s) | 192 |
| NmuCI | GTSAC | 2 cut(s) | 12, 646 |
| NspI | RCATGY | 1 cut(s) | 59 |
| PaqCI | CACCTGC | 1 cut(s) | 761 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 244 |
| PfeI | GAWTC | 4 cut(s) | 235, 249, 566, 720 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 398 |
| PkrI | GCNGC | 1 cut(s) | 730 |
| PleI | GAGTC | 2 cut(s) | 65, 167 |
| PpsI | GAGTC | 2 cut(s) | 65, 167 |
| Ppu21I | YACGTR | 1 cut(s) | 823 |
| PpuMI | RGGWCCY | 1 cut(s) | 407 |
| Psp5II | RGGWCCY | 1 cut(s) | 407 |
| Psp6I | CCWGG | 1 cut(s) | 409 |
| PspGI | CCWGG | 1 cut(s) | 409 |
| PspN4I | GGNNCC | 1 cut(s) | 192 |
| PspPI | GGNCC | 1 cut(s) | 407 |
| PspPPI | RGGWCCY | 1 cut(s) | 407 |
| PsuI | RGATCY | 1 cut(s) | 109 |
| PvuII | CAGCTG | 1 cut(s) | 143 |
| RsaI | GTAC | 3 cut(s) | 354, 594, 691 |
| RsaNI | GTAC | 3 cut(s) | 353, 593, 690 |
| RseI | CAYNNNNRTG | 1 cut(s) | 621 |
| SaqAI | TTAA | 1 cut(s) | 437 |
| SatI | GCNGC | 1 cut(s) | 729 |
| Sau3AI | GATC | 3 cut(s) | 33, 109, 552 |
| Sau96I | GGNCC | 1 cut(s) | 407 |
| SchI | GAGTC | 2 cut(s) | 65, 167 |
| ScrFI | CCNGG | 2 cut(s) | 65, 411 |
| SexAI | ACCWGGT | 1 cut(s) | 409 |
| SfaNI | GCATC | 2 cut(s) | 407, 470 |
| SinI | GGWCC | 1 cut(s) | 407 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 621 |
| SmlI | CTYRAG | 1 cut(s) | 185 |
| SmoI | CTYRAG | 1 cut(s) | 185 |
| SnaBI | TACGTA | 1 cut(s) | 823 |
| Sse9I | AATT | 6 cut(s) | 160, 196, 393, 433, 520, 761 |
| SsiI | CCGC | 1 cut(s) | 201 |
| StyD4I | CCNGG | 2 cut(s) | 63, 409 |
| TaaI | ACNGT | 3 cut(s) | 276, 368, 682 |
| TaiI | ACGT | 2 cut(s) | 808, 825 |
| TaqI | TCGA | 2 cut(s) | 612, 696 |
| TasI | AATT | 6 cut(s) | 160, 196, 393, 433, 520, 761 |
| TatI | WGTACW | 2 cut(s) | 352, 689 |
| TfiI | GAWTC | 4 cut(s) | 235, 249, 566, 720 |
| Tru1I | TTAA | 1 cut(s) | 437 |
| Tru9I | TTAA | 1 cut(s) | 437 |
| TscAI | CASTG | 1 cut(s) | 371 |
| TseFI | GTSAC | 2 cut(s) | 12, 646 |
| TseI | GCWGC | 1 cut(s) | 728 |
| Tsp45I | GTSAC | 2 cut(s) | 12, 646 |
| TspDTI | ATGAA | 4 cut(s) | 301, 342, 514, 674 |
| TspRI | CASTG | 1 cut(s) | 371 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 398 |
| VpaK11BI | GGWCC | 1 cut(s) | 407 |
| XceI | RCATGY | 1 cut(s) | 59 |
| XmiI | GTMKAC | 3 cut(s) | 425, 819, 825 |