Rw7G004170

B3 domain-containing

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000407
Physical Location & Seq
Reverse (-)
3469896 .. 3471791
1896 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G004170.1

Sequence Viewer

Length: 906 bp
ATGGCCACATTCTCACGACCTATGGCTTCTTCAATTGCGAGACGTGCCCTAATTCCAGAGGACCCTCCAAGCTTCTGCATTAAGATTGTCACTGATAATGACCTCCGAGATGGGAAGGAACTTCCCGAGATGGCTGTAAACATATATCGAAACTGTATGGAAGACTCTATATTCCTCAAGGTACCAAATTGTGAGACATTATGGCCAATTGAAGTGAAAAAATCAGCTCATGGTGGCCGAATGTGGTTGCACAAGGGATGGCAAGAGTTTGCCAACTTTTACTCACTAGAGCAAGGATACTTGGTATTTGTGAGTTATAAAGGCGAGCATTCTCATTTCCAAGGACGCATTTTCCATTGGAATGATGTGGAAATCGAGTACCCTATTCGCGGATCAGGAGGAACTGATGGCATACCAAAAAGGAAAAGAACAAGAGCTCATGGTGGCATGCCTAATGCTTATTTAAAAGCAGCTAGAGCCATTTCAAATTCCTCCGGATCCTCGGGTAATGAACACCTGAATGTTCTTTCCACAGCTAACAGTTTCAAATCTGACAAGCCATTCATTATGATCAAAATGACAGCTTATTACTTGCGTAGAGAAATGTTTATAAGTGCATCTTTTGCCACAAAACATCTCTGTCGAGCAGGCGCTTCTTGTGACTTGTCCCTACAGACTCCAGATATGAGAACTTGGATTGCTGAATTGATTGTGTATGAAACCACTGAGTCAAGAGTATTTGCAAAGATTAAAGGTGCTGGTTGGAAAGCATTTAAGGAGGAAAATCATCTCGAAGAAGGTGATGTCTGTGTGCTTGAGTTGATAGAGGAATGTAAATTCAGAGTTTCGATATTCCGAGCAAGAGTGAAGATTAACGAGTTAAGTGAAGCTGGAAGGCCAAGATGA

Protein Analysis

301

Amino Acids

34.24

Weight (kDa)

8.87

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 191 - 287 2.1e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000407)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29511 FvH4_5g16850 FvH4_5g16850 FvH4_5g16850 FvH4_5g16850 FvH4_6g40050 FvH4_7g32411 FvH4_7g32411 FvH4_7g32412 FvH4_7g32412 FvH4_7g32412 FvH4_7g32412 FvH4_7g32412 FvH4_7g32750
rosa_chinensis RchiOBHm_Chr1g0382001 RchiOBHm_Chr1g0382011 RchiOBHm_Chr7g0179221 RchiOBHm_Chr7g0182451 RchiOBHm_Chr7g0182461 RchiOBHm_Chr7g0182471 RchiOBHm_Chr7g0182511 RchiOBHm_Chr7g0211791
rosa_laevigata RLG00000002969 RLG00000005122 RLG00000005123 RLG00000026188 RLG00000026193 RLG00000026194
rosa_multiflora Rmu_co8485709.1_g000001 Rmu_sc0000110.1_g000003 Rmu_sc0006047.1_g000007 Rmu_sc0006047.1_g000011 Rmu_sc0006047.1_g000015 Rmu_sc0006047.1_g000016 Rmu_sc0007448.1_g000001 Rmu_sc0009005.1_g000015 Rmu_sc0009991.1_g000003 Rmu_sc0016160.1_g000004 Rmu_sc0030974.1_g000004 Rmu_sc0030974.1_g000005 Rmu_sc0034215.1_g000004
rosa_roxburghii Rroxscaffold_3G00247430 Rroxscaffold_3G00271240 Rroxscaffold_3G00271250 Rroxscaffold_3G00274210 Rroxscaffold_4G00278270
rosa_rugosa Rorug01G0432800 Rorug01G0432900 Rorug01G0432900 Rorug01G0432900 Rorug01G0433000 Rorug06G0422300 Rorug06G0422400 Rorug06G0447800 Rorug06G0447900 Rorug06G0448000 Rorug07G0128700
rosa_samantha Rh1AG456100 Rh1AG456200 Rh1AG456500 Rh1AG457000 Rh1AG457600 Rh1AG457700 Rh1BG413200 Rh1CG428100 Rh1CG428200 Rh1DG445500 Rh2DG673100 Rh7AG021600 Rh7AG051100 Rh7AG051200 Rh7AG051300 Rh7AG051600 Rh7AG261400 Rh7BG021600 Rh7BG050700 Rh7BG050800 Rh7BG255000 Rh7CG022800 Rh7CG052200 Rh7CG052300 Rh7CG052400 Rh7CG052500 Rh7CG278000 Rh7DG022200 Rh7DG050000 Rh7DG050100 Rh7DG050200 Rh7DG050600 Rh7DG050700 Rh7DG268000
rosa_wichuraiana Rw1G041300 Rw1G041310 Rw1G041540 Rw1G041550 Rw7G001780 Rw7G004150 Rw7G004160 Rw7G004170 Rw7G022290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 318, 611
Acc65I GGTACC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 181
AccII CGCG 1 cut(s) 390
AccIII TCCGGA 1 cut(s) 494
AciI CCGC 1 cut(s) 390
AclWI GGATC 3 cut(s) 400, 492, 505
AcoI YGGCCR 3 cut(s) 3, 203, 235
AcsI RAATTY 2 cut(s) 487, 836
AfaI GTAC 2 cut(s) 183, 380
AfiI CCNNNNNNNGG 1 cut(s) 389
AgsI TTSAA 4 cut(s) 33, 212, 486, 547
AjiI CACGTC 1 cut(s) 44
AluBI AGCT 7 cut(s) 72, 227, 437, 473, 536, 584, 890
AluI AGCT 7 cut(s) 72, 227, 437, 473, 536, 584, 890
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 2 cut(s) 34, 188
AlwI GGATC 3 cut(s) 400, 492, 505
Ama87I CYCGRG 2 cut(s) 125, 502
Aor13HI TCCGGA 1 cut(s) 494
AoxI GGCC 4 cut(s) 3, 203, 235, 896
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 2 cut(s) 487, 836
Asp718I GGTACC 1 cut(s) 181
AspLEI GCGC 1 cut(s) 653
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 1 cut(s) 812
AvaI CYCGRG 2 cut(s) 125, 502
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 49
BalI TGGCCA 2 cut(s) 5, 205
BamHI GGATCC 1 cut(s) 497
BanI GGYRCC 1 cut(s) 181
BanII GRGCYC 1 cut(s) 439
BbsI GAAGAC 1 cut(s) 168
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 482
BccI CCATC 4 cut(s) 104, 124, 252, 401
BciVI GTATCC 1 cut(s) 290
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 2 cut(s) 34, 188
BfaI CTAG 2 cut(s) 287, 474
BfmI CTRYAG 1 cut(s) 671
BfoI RGCGCY 1 cut(s) 654
BfuI GTATCC 1 cut(s) 290
BisI GCNGC 1 cut(s) 471
BlsI GCNGC 1 cut(s) 472
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 125, 502
BmgBI CACGTC 1 cut(s) 44
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 3 cut(s) 63, 183, 499
BmsI GCATC 1 cut(s) 626
BpiI GAAGAC 1 cut(s) 168
BpmI CTGGAG 1 cut(s) 663
BpuEI CTTGAG 2 cut(s) 161, 836
BsaJI CCNNGG 2 cut(s) 340, 501
BsaWI WCCGGW 1 cut(s) 494
Bsc4I CCNNNNNNNGG 1 cut(s) 389
BseAI TCCGGA 1 cut(s) 494
BseDI CCNNGG 2 cut(s) 340, 501
BseGI GGATG 1 cut(s) 263
BseLI CCNNNNNNNGG 1 cut(s) 389
BseMII CTCAG 1 cut(s) 717
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 1 cut(s) 482
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 4 cut(s) 5, 205, 237, 898
BshNI GGYRCC 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 2 cut(s) 125, 502
BsiSI CCGG 1 cut(s) 495
BslFI GGGAC 1 cut(s) 652
BslI CCNNNNNNNGG 1 cut(s) 389
BsmAI GTCTC 2 cut(s) 34, 188
BsmBI CGTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 652
BsmI GAATGC 1 cut(s) 328
BsnI GGCC 4 cut(s) 5, 205, 237, 898
BsoBI CYCGRG 2 cut(s) 125, 502
Bsp1286I GDGCHC 2 cut(s) 49, 439
Bsp13I TCCGGA 1 cut(s) 494
Bsp143I GATC 3 cut(s) 392, 497, 570
BspACI CCGC 1 cut(s) 390
BspANI GGCC 4 cut(s) 5, 205, 237, 898
BspCNI CTCAG 1 cut(s) 718
BspEI TCCGGA 1 cut(s) 494
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 3 cut(s) 63, 183, 499
BspPI GGATC 3 cut(s) 400, 492, 505
BspT107I GGYRCC 1 cut(s) 181
BssECI CCNNGG 2 cut(s) 340, 501
BssMI GATC 3 cut(s) 392, 497, 570
BssT1I CCWWGG 1 cut(s) 340
Bst4CI ACNGT 2 cut(s) 155, 542
BstAPI GCANNNNNTGC 1 cut(s) 623
BstC8I GCNNGC 3 cut(s) 326, 449, 649
BstDEI CTNAG 1 cut(s) 726
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 654
BstHHI GCGC 1 cut(s) 653
BstKTI GATC 3 cut(s) 395, 500, 573
BstMAI GTCTC 2 cut(s) 34, 188
BstMBI GATC 3 cut(s) 392, 497, 570
BstMWI GCNNNNNNNGC 3 cut(s) 44, 476, 623
BstNSI RCATGY 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 671
BstSLI GKGCMC 1 cut(s) 49
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 168
BstX2I RGATCY 1 cut(s) 497
BstYI RGATCY 1 cut(s) 497
BsuI GTATCC 1 cut(s) 290
BsuRI GGCC 4 cut(s) 5, 205, 237, 898
BtrI CACGTC 1 cut(s) 44
BtsCI GGATG 1 cut(s) 263
BtsIMutI CAGTG 2 cut(s) 90, 723
Cac8I GCNNGC 3 cut(s) 326, 449, 649
CfoI GCGC 1 cut(s) 653
Cfr13I GGNCC 1 cut(s) 61
CseI GACGC 1 cut(s) 354
Csp6I GTAC 2 cut(s) 182, 379
CviAII CATG 3 cut(s) 230, 440, 448
CviQI GTAC 2 cut(s) 182, 379
DdeI CTNAG 1 cut(s) 726
DpnI GATC 3 cut(s) 394, 499, 572
DpnII GATC 3 cut(s) 392, 497, 570
DraI TTTAAA 1 cut(s) 465
EaeI YGGCCR 3 cut(s) 3, 203, 235
Ecl136II GAGCTC 1 cut(s) 437
Eco130I CCWWGG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 439
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 437
Eco88I CYCGRG 2 cut(s) 125, 502
EcoICRI GAGCTC 1 cut(s) 437
EcoO109I RGGNCCY 1 cut(s) 61
EcoT14I CCWWGG 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 340
Esp3I CGTCTC 1 cut(s) 34
FaeI CATG 3 cut(s) 233, 443, 451
FalI AAGNNNNNCTT 2 cut(s) 604, 636
FaqI GGGAC 1 cut(s) 652
FatI CATG 3 cut(s) 229, 439, 447
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 1 cut(s) 471
FokI GGATG 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 1 cut(s) 471
FspBI CTAG 2 cut(s) 287, 474
GlaI GCGC 1 cut(s) 652
GluI GCNGC 1 cut(s) 471
GsuI CTGGAG 1 cut(s) 663
HaeII RGCGCY 1 cut(s) 654
HaeIII GGCC 4 cut(s) 5, 205, 237, 898
HapII CCGG 1 cut(s) 495
HgaI GACGC 1 cut(s) 354
HhaI GCGC 1 cut(s) 653
Hin1II CATG 3 cut(s) 233, 443, 451
Hin6I GCGC 1 cut(s) 651
HinP1I GCGC 1 cut(s) 651
HindIII AAGCTT 1 cut(s) 70
HinfI GANTC 3 cut(s) 164, 676, 728
HpaII CCGG 1 cut(s) 495
HphI GGTGA 1 cut(s) 812
Hpy166II GTNNAC 1 cut(s) 139
Hpy188I TCNGA 4 cut(s) 107, 553, 842, 857
Hpy188III TCNNGA 8 cut(s) 15, 56, 125, 396, 495, 680, 732, 791
Hpy8I GTNNAC 1 cut(s) 139
HpyAV CCTTC 3 cut(s) 109, 791, 888
HpyCH4III ACNGT 2 cut(s) 155, 542
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 4 cut(s) 78, 250, 617, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 44, 476, 623
HpyF3I CTNAG 1 cut(s) 726
HpySE526I ACGT 1 cut(s) 43
Hsp92II CATG 3 cut(s) 233, 443, 451
HspAI GCGC 1 cut(s) 651
Kpn2I TCCGGA 1 cut(s) 494
KpnI GGTACC 1 cut(s) 185
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 3 cut(s) 392, 497, 570
LpnPI CCDG 8 cut(s) 69, 381, 508, 530, 633, 693, 744, 876
Lsp1109I GCAGC 1 cut(s) 482
LweI GCATC 1 cut(s) 626
MaeI CTAG 2 cut(s) 287, 474
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 2 cut(s) 88, 659
MalI GATC 3 cut(s) 394, 499, 572
MboI GATC 3 cut(s) 392, 497, 570
MboII GAAGA 4 cut(s) 21, 173, 806, 880
MfeI CAATTG 2 cut(s) 33, 207
MflI RGATCY 1 cut(s) 497
MhlI GDGCHC 2 cut(s) 49, 439
MlsI TGGCCA 2 cut(s) 5, 205
MluCI AATT 7 cut(s) 33, 51, 187, 207, 487, 704, 836
MluNI TGGCCA 2 cut(s) 5, 205
MlyI GAGTC 3 cut(s) 158, 670, 737
MmeI TCCRAC 1 cut(s) 743
MnlI CCTC 9 cut(s) 52, 75, 113, 185, 392, 502, 511, 772, 820
Mox20I TGGCCA 2 cut(s) 5, 205
MroI TCCGGA 1 cut(s) 494
MscI TGGCCA 2 cut(s) 5, 205
MseI TTAA 6 cut(s) 81, 464, 750, 774, 873, 881
MslI CAYNNNNRTG 2 cut(s) 360, 519
Msp20I TGGCCA 2 cut(s) 5, 205
MspI CCGG 1 cut(s) 495
MunI CAATTG 2 cut(s) 33, 207
Mva1269I GAATGC 1 cut(s) 328
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 3 cut(s) 44, 476, 623
NdeII GATC 3 cut(s) 392, 497, 570
NlaIII CATG 3 cut(s) 233, 443, 451
NlaIV GGNNCC 3 cut(s) 63, 183, 499
NmuCI GTSAC 2 cut(s) 88, 659
NspI RCATGY 1 cut(s) 451
PaeI GCATGC 1 cut(s) 451
PctI GAATGC 1 cut(s) 328
PkrI GCNGC 1 cut(s) 472
PleI GAGTC 3 cut(s) 158, 670, 736
PpsI GAGTC 3 cut(s) 158, 670, 736
PpuMI RGGWCCY 1 cut(s) 61
PsiI TTATAA 2 cut(s) 318, 611
Psp124BI GAGCTC 1 cut(s) 439
Psp5II RGGWCCY 1 cut(s) 61
PspN4I GGNNCC 3 cut(s) 63, 183, 499
PspPI GGNCC 1 cut(s) 61
PspPPI RGGWCCY 1 cut(s) 61
PsuI RGATCY 1 cut(s) 497
RsaI GTAC 2 cut(s) 183, 380
RsaNI GTAC 2 cut(s) 182, 379
RseI CAYNNNNRTG 2 cut(s) 360, 519
SacI GAGCTC 1 cut(s) 439
SaqAI TTAA 6 cut(s) 81, 464, 750, 774, 873, 881
SatI GCNGC 1 cut(s) 471
Sau3AI GATC 3 cut(s) 392, 497, 570
Sau96I GGNCC 1 cut(s) 61
SchI GAGTC 3 cut(s) 158, 670, 737
SduI GDGCHC 2 cut(s) 49, 439
SfaNI GCATC 1 cut(s) 626
SfcI CTRYAG 1 cut(s) 671
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 2 cut(s) 360, 519
SmlI CTYRAG 2 cut(s) 176, 815
SmoI CTYRAG 2 cut(s) 176, 815
SphI GCATGC 1 cut(s) 451
Sse9I AATT 7 cut(s) 33, 51, 187, 207, 487, 704, 836
SsiI CCGC 1 cut(s) 390
SspMI CTAG 2 cut(s) 287, 474
SstI GAGCTC 1 cut(s) 439
StyI CCWWGG 1 cut(s) 340
TaaI ACNGT 2 cut(s) 155, 542
TaiI ACGT 1 cut(s) 46
TaqI TCGA 5 cut(s) 148, 375, 643, 792, 848
TasI AATT 7 cut(s) 33, 51, 187, 207, 487, 704, 836
Tru1I TTAA 6 cut(s) 81, 464, 750, 774, 873, 881
Tru9I TTAA 6 cut(s) 81, 464, 750, 774, 873, 881
TscAI CASTG 2 cut(s) 97, 730
TseFI GTSAC 2 cut(s) 88, 659
TseI GCWGC 1 cut(s) 470
Tsp45I GTSAC 2 cut(s) 88, 659
TspDTI ATGAA 3 cut(s) 525, 553, 732
TspRI CASTG 2 cut(s) 97, 730
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 487, 836
XceI RCATGY 1 cut(s) 451
XspI CTAG 2 cut(s) 287, 474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.