B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 674 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 188 |
| AciI | CCGC | 1 cut(s) | 122 |
| AclWI | GGATC | 2 cut(s) | 465, 538 |
| AcsI | RAATTY | 2 cut(s) | 325, 639 |
| AcuI | CTGAAG | 3 cut(s) | 336, 456, 561 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 188 |
| AflII | CTTAAG | 1 cut(s) | 173 |
| AgsI | TTSAA | 1 cut(s) | 558 |
| AjnI | CCWGG | 1 cut(s) | 855 |
| AlwI | GGATC | 2 cut(s) | 465, 538 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 824 |
| AoxI | GGCC | 1 cut(s) | 657 |
| ApeKI | GCWGC | 1 cut(s) | 128 |
| ApoI | RAATTY | 2 cut(s) | 325, 639 |
| AsuHPI | GGTGA | 5 cut(s) | 213, 761, 845, 878, 898 |
| BbsI | GAAGAC | 1 cut(s) | 514 |
| BbvI | GCAGC | 1 cut(s) | 140 |
| BccI | CCATC | 1 cut(s) | 249 |
| BceAI | ACGGC | 1 cut(s) | 29 |
| BciT130I | CCWGG | 1 cut(s) | 857 |
| BfaI | CTAG | 4 cut(s) | 284, 443, 546, 881 |
| BfmI | CTRYAG | 3 cut(s) | 349, 447, 734 |
| BfrI | CTTAAG | 1 cut(s) | 173 |
| BglII | AGATCT | 1 cut(s) | 94 |
| BisI | GCNGC | 1 cut(s) | 129 |
| BlsI | GCNGC | 1 cut(s) | 130 |
| Bme1390I | CCNGG | 1 cut(s) | 857 |
| BmiI | GGNNCC | 1 cut(s) | 180 |
| BmrFI | CCNGG | 1 cut(s) | 857 |
| BmsI | GCATC | 1 cut(s) | 354 |
| BpiI | GAAGAC | 1 cut(s) | 514 |
| BpmI | CTGGAG | 1 cut(s) | 45 |
| Bpu10I | CCTNAGC | 1 cut(s) | 498 |
| BpuEI | CTTGAG | 2 cut(s) | 94, 519 |
| BsaJI | CCNNGG | 2 cut(s) | 289, 654 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 387, 417 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 188 |
| Bse118I | RCCGGY | 1 cut(s) | 124 |
| BseBI | CCWGG | 1 cut(s) | 857 |
| BseDI | CCNNGG | 2 cut(s) | 289, 654 |
| BseGI | GGATG | 2 cut(s) | 260, 364 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 188 |
| BseMII | CTCAG | 1 cut(s) | 705 |
| BseRI | GAGGAG | 1 cut(s) | 408 |
| BseXI | GCAGC | 1 cut(s) | 140 |
| BsgI | GTGCAG | 1 cut(s) | 925 |
| BshFI | GGCC | 1 cut(s) | 659 |
| BsiSI | CCGG | 1 cut(s) | 125 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 188 |
| BsnI | GGCC | 1 cut(s) | 659 |
| Bsp143I | GATC | 4 cut(s) | 94, 470, 530, 569 |
| BspACI | CCGC | 1 cut(s) | 122 |
| BspANI | GGCC | 1 cut(s) | 659 |
| BspCNI | CTCAG | 1 cut(s) | 704 |
| BspLI | GGNNCC | 1 cut(s) | 180 |
| BspPI | GGATC | 2 cut(s) | 465, 538 |
| BspTI | CTTAAG | 1 cut(s) | 173 |
| BsrFI | RCCGGY | 1 cut(s) | 124 |
| BssAI | RCCGGY | 1 cut(s) | 124 |
| BssECI | CCNNGG | 2 cut(s) | 289, 654 |
| BssMI | GATC | 4 cut(s) | 94, 470, 530, 569 |
| BssT1I | CCWWGG | 2 cut(s) | 289, 654 |
| Bst2UI | CCWGG | 1 cut(s) | 857 |
| Bst6I | CTCTTC | 1 cut(s) | 255 |
| BstAFI | CTTAAG | 1 cut(s) | 173 |
| BstC8I | GCNNGC | 1 cut(s) | 126 |
| BstDEI | CTNAG | 2 cut(s) | 498, 691 |
| BstF5I | GGATG | 2 cut(s) | 260, 364 |
| BstKTI | GATC | 4 cut(s) | 97, 473, 533, 572 |
| BstMBI | GATC | 4 cut(s) | 94, 470, 530, 569 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 318 |
| BstNI | CCWGG | 1 cut(s) | 857 |
| BstNSI | RCATGY | 1 cut(s) | 44 |
| BstSCI | CCNGG | 1 cut(s) | 855 |
| BstSFI | CTRYAG | 3 cut(s) | 349, 447, 734 |
| BstV1I | GCAGC | 1 cut(s) | 140 |
| BstV2I | GAAGAC | 1 cut(s) | 514 |
| BstX2I | RGATCY | 3 cut(s) | 94, 470, 530 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 655 |
| BstYI | RGATCY | 3 cut(s) | 94, 470, 530 |
| BsuRI | GGCC | 1 cut(s) | 659 |
| BtsCI | GGATG | 2 cut(s) | 260, 364 |
| BtsIMutI | CAGTG | 1 cut(s) | 776 |
| Cac8I | GCNNGC | 1 cut(s) | 126 |
| CaiI | CAGNNNCTG | 1 cut(s) | 824 |
| Cfr10I | RCCGGY | 1 cut(s) | 124 |
| CseI | GACGC | 1 cut(s) | 739 |
| CviAII | CATG | 2 cut(s) | 41, 298 |
| DdeI | CTNAG | 2 cut(s) | 498, 691 |
| DpnI | GATC | 4 cut(s) | 96, 472, 532, 571 |
| DpnII | GATC | 4 cut(s) | 94, 470, 530, 569 |
| Eam1104I | CTCTTC | 1 cut(s) | 255 |
| EarI | CTCTTC | 1 cut(s) | 255 |
| EciI | GGCGGA | 1 cut(s) | 111 |
| Eco130I | CCWWGG | 2 cut(s) | 289, 654 |
| Eco32I | GATATC | 1 cut(s) | 634 |
| Eco57I | CTGAAG | 3 cut(s) | 336, 456, 561 |
| EcoRI | GAATTC | 1 cut(s) | 639 |
| EcoRII | CCWGG | 1 cut(s) | 855 |
| EcoRV | GATATC | 1 cut(s) | 634 |
| EcoT14I | CCWWGG | 2 cut(s) | 289, 654 |
| ErhI | CCWWGG | 2 cut(s) | 289, 654 |
| FaeI | CATG | 2 cut(s) | 44, 301 |
| FatI | CATG | 2 cut(s) | 40, 297 |
| Fnu4HI | GCNGC | 1 cut(s) | 129 |
| FokI | GGATG | 2 cut(s) | 267, 371 |
| Fsp4HI | GCNGC | 1 cut(s) | 129 |
| FspBI | CTAG | 4 cut(s) | 284, 443, 546, 881 |
| GluI | GCNGC | 1 cut(s) | 129 |
| GsuI | CTGGAG | 1 cut(s) | 45 |
| HaeIII | GGCC | 1 cut(s) | 659 |
| HapII | CCGG | 1 cut(s) | 125 |
| HgaI | GACGC | 1 cut(s) | 739 |
| Hin1II | CATG | 2 cut(s) | 44, 301 |
| HindIII | AAGCTT | 1 cut(s) | 681 |
| HpaII | CCGG | 1 cut(s) | 125 |
| HphI | GGTGA | 5 cut(s) | 213, 761, 845, 878, 898 |
| Hpy188I | TCNGA | 6 cut(s) | 316, 506, 616, 638, 694, 923 |
| Hpy188III | TCNNGA | 4 cut(s) | 50, 89, 230, 629 |
| Hpy99I | CGWCG | 1 cut(s) | 231 |
| HpyAV | CCTTC | 6 cut(s) | 76, 106, 244, 311, 510, 857 |
| HpyCH4IV | ACGT | 2 cut(s) | 487, 898 |
| HpyCH4V | TGCA | 1 cut(s) | 906 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 318 |
| HpyF3I | CTNAG | 2 cut(s) | 498, 691 |
| HpySE526I | ACGT | 2 cut(s) | 487, 898 |
| Hsp92II | CATG | 2 cut(s) | 44, 301 |
| KroI | GCCGGC | 1 cut(s) | 124 |
| KroNI | GCCGGC | 1 cut(s) | 126 |
| Kzo9I | GATC | 4 cut(s) | 94, 470, 530, 569 |
| LmnI | GCTCC | 3 cut(s) | 20, 395, 431 |
| Lsp1109I | GCAGC | 1 cut(s) | 140 |
| LweI | GCATC | 1 cut(s) | 354 |
| MaeI | CTAG | 4 cut(s) | 284, 443, 546, 881 |
| MaeII | ACGT | 2 cut(s) | 487, 898 |
| MaeIII | GTNAC | 5 cut(s) | 230, 523, 722, 749, 791 |
| MalI | GATC | 4 cut(s) | 96, 472, 532, 571 |
| MboI | GATC | 4 cut(s) | 94, 470, 530, 569 |
| MboII | GAAGA | 7 cut(s) | 104, 121, 272, 519, 525, 810, 872 |
| MflI | RGATCY | 3 cut(s) | 94, 470, 530 |
| MluCI | AATT | 6 cut(s) | 184, 325, 558, 639, 765, 813 |
| MmeI | TCCRAC | 2 cut(s) | 594, 809 |
| MnlI | CCTC | 8 cut(s) | 69, 129, 234, 386, 484, 700, 877, 886 |
| MroNI | GCCGGC | 1 cut(s) | 124 |
| MseI | TTAA | 1 cut(s) | 174 |
| MslI | CAYNNNNRTG | 1 cut(s) | 591 |
| MspA1I | CMGCKG | 1 cut(s) | 131 |
| MspCI | CTTAAG | 1 cut(s) | 173 |
| MspI | CCGG | 1 cut(s) | 125 |
| MspR9I | CCNGG | 1 cut(s) | 857 |
| MvaI | CCWGG | 1 cut(s) | 857 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 318 |
| NaeI | GCCGGC | 1 cut(s) | 126 |
| NdeII | GATC | 4 cut(s) | 94, 470, 530, 569 |
| NgoMIV | GCCGGC | 1 cut(s) | 124 |
| NlaIII | CATG | 2 cut(s) | 44, 301 |
| NlaIV | GGNNCC | 1 cut(s) | 180 |
| NmuCI | GTSAC | 3 cut(s) | 230, 722, 749 |
| NspI | RCATGY | 1 cut(s) | 44 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 232 |
| PdiI | GCCGGC | 1 cut(s) | 126 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 188 |
| PkrI | GCNGC | 1 cut(s) | 130 |
| PsiI | TTATAA | 1 cut(s) | 674 |
| Psp6I | CCWGG | 1 cut(s) | 855 |
| PspGI | CCWGG | 1 cut(s) | 855 |
| PspN4I | GGNNCC | 1 cut(s) | 180 |
| PstNI | CAGNNNCTG | 1 cut(s) | 824 |
| PsuI | RGATCY | 3 cut(s) | 94, 470, 530 |
| PvuII | CAGCTG | 1 cut(s) | 131 |
| RseI | CAYNNNNRTG | 1 cut(s) | 591 |
| SaqAI | TTAA | 1 cut(s) | 174 |
| SatI | GCNGC | 1 cut(s) | 129 |
| Sau3AI | GATC | 4 cut(s) | 94, 470, 530, 569 |
| ScrFI | CCNGG | 1 cut(s) | 857 |
| SfaNI | GCATC | 1 cut(s) | 354 |
| SfcI | CTRYAG | 3 cut(s) | 349, 447, 734 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 591 |
| SmlI | CTYRAG | 3 cut(s) | 73, 173, 534 |
| SmoI | CTYRAG | 3 cut(s) | 73, 173, 534 |
| Sse9I | AATT | 6 cut(s) | 184, 325, 558, 639, 765, 813 |
| SsiI | CCGC | 1 cut(s) | 122 |
| SspMI | CTAG | 4 cut(s) | 284, 443, 546, 881 |
| StyD4I | CCNGG | 1 cut(s) | 855 |
| StyI | CCWWGG | 2 cut(s) | 289, 654 |
| TaiI | ACGT | 2 cut(s) | 490, 901 |
| TaqI | TCGA | 1 cut(s) | 914 |
| TaqII | GACCGA | 1 cut(s) | 249 |
| TasI | AATT | 6 cut(s) | 184, 325, 558, 639, 765, 813 |
| Tru1I | TTAA | 1 cut(s) | 174 |
| Tru9I | TTAA | 1 cut(s) | 174 |
| TscAI | CASTG | 1 cut(s) | 776 |
| TseFI | GTSAC | 3 cut(s) | 230, 722, 749 |
| TseI | GCWGC | 1 cut(s) | 128 |
| Tsp45I | GTSAC | 3 cut(s) | 230, 722, 749 |
| TspDTI | ATGAA | 3 cut(s) | 498, 597, 768 |
| TspGWI | ACGGA | 1 cut(s) | 810 |
| TspRI | CASTG | 1 cut(s) | 776 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 188 |
| Vha464I | CTTAAG | 1 cut(s) | 173 |
| XapI | RAATTY | 2 cut(s) | 325, 639 |
| XceI | RCATGY | 1 cut(s) | 44 |
| XspI | CTAG | 4 cut(s) | 284, 443, 546, 881 |