B3 domain-containing
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 462 |
| AccI | GTMKAC | 1 cut(s) | 400 |
| AccII | CGCG | 1 cut(s) | 611 |
| AccIII | TCCGGA | 1 cut(s) | 497 |
| AciI | CCGC | 1 cut(s) | 483 |
| AclWI | GGATC | 2 cut(s) | 495, 508 |
| AcsI | RAATTY | 3 cut(s) | 59, 71, 682 |
| AdeI | CACNNNGTG | 1 cut(s) | 742 |
| AfaI | GTAC | 1 cut(s) | 354 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 200 |
| AgsI | TTSAA | 4 cut(s) | 188, 489, 550, 599 |
| AjiI | CACGTC | 1 cut(s) | 17 |
| AjnI | CCWGG | 2 cut(s) | 763, 905 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 294, 326 |
| AluBI | AGCT | 9 cut(s) | 26, 81, 143, 236, 324, 443, 509, 539, 732 |
| AluI | AGCT | 9 cut(s) | 26, 81, 143, 236, 324, 443, 509, 539, 732 |
| Alw26I | GTCTC | 1 cut(s) | 131 |
| AlwI | GGATC | 2 cut(s) | 495, 508 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 764 |
| Aor13HI | TCCGGA | 1 cut(s) | 497 |
| AoxI | GGCC | 1 cut(s) | 761 |
| ApeKI | GCWGC | 3 cut(s) | 306, 536, 539 |
| ApoI | RAATTY | 3 cut(s) | 59, 71, 682 |
| AspS9I | GGNCC | 1 cut(s) | 761 |
| AsuHPI | GGTGA | 2 cut(s) | 728, 818 |
| BamHI | GGATCC | 1 cut(s) | 500 |
| BbvCI | CCTCAGC | 1 cut(s) | 505 |
| BbvI | GCAGC | 3 cut(s) | 293, 526, 548 |
| BccI | CCATC | 2 cut(s) | 113, 261 |
| BceAI | ACGGC | 1 cut(s) | 430 |
| BciT130I | CCWGG | 2 cut(s) | 765, 907 |
| BclI | TGATCA | 1 cut(s) | 172 |
| BcoDI | GTCTC | 1 cut(s) | 131 |
| BfaI | CTAG | 2 cut(s) | 296, 477 |
| BfmI | CTRYAG | 2 cut(s) | 534, 677 |
| BfuAI | ACCTGC | 1 cut(s) | 462 |
| BisI | GCNGC | 4 cut(s) | 307, 483, 537, 540 |
| BlsI | GCNGC | 4 cut(s) | 308, 484, 538, 541 |
| Bme1390I | CCNGG | 2 cut(s) | 765, 907 |
| BmgBI | CACGTC | 1 cut(s) | 17 |
| BmgT120I | GGNCC | 1 cut(s) | 761 |
| BmiI | GGNNCC | 1 cut(s) | 502 |
| BmrFI | CCNGG | 2 cut(s) | 765, 907 |
| BmsI | GCATC | 1 cut(s) | 632 |
| Bpu10I | CCTNAGC | 1 cut(s) | 505 |
| BsaBI | GATNNNNATC | 1 cut(s) | 516 |
| BsaJI | CCNNGG | 3 cut(s) | 209, 301, 862 |
| BsaWI | WCCGGW | 1 cut(s) | 497 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 200 |
| Bse1I | ACTGG | 2 cut(s) | 314, 543 |
| Bse3DI | GCAATG | 1 cut(s) | 673 |
| Bse8I | GATNNNNATC | 1 cut(s) | 516 |
| BseAI | TCCGGA | 1 cut(s) | 497 |
| BseBI | CCWGG | 2 cut(s) | 765, 907 |
| BseDI | CCNNGG | 3 cut(s) | 209, 301, 862 |
| BseGI | GGATG | 1 cut(s) | 272 |
| BseJI | GATNNNNATC | 1 cut(s) | 516 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 200 |
| BseMI | GCAATG | 1 cut(s) | 673 |
| BseMII | CTCAG | 5 cut(s) | 127, 238, 519, 722, 932 |
| BseNI | ACTGG | 2 cut(s) | 314, 543 |
| BseRI | GAGGAG | 2 cut(s) | 420, 704 |
| BseXI | GCAGC | 3 cut(s) | 293, 526, 548 |
| Bsh1236I | CGCG | 1 cut(s) | 611 |
| BshFI | GGCC | 1 cut(s) | 763 |
| BsiSI | CCGG | 1 cut(s) | 498 |
| BslFI | GGGAC | 1 cut(s) | 325 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 200 |
| BsmAI | GTCTC | 1 cut(s) | 131 |
| BsmFI | GGGAC | 1 cut(s) | 325 |
| BsnI | GGCC | 1 cut(s) | 763 |
| Bsp13I | TCCGGA | 1 cut(s) | 497 |
| Bsp143I | GATC | 2 cut(s) | 172, 500 |
| Bsp19I | CCATGG | 1 cut(s) | 209 |
| BspACI | CCGC | 1 cut(s) | 483 |
| BspANI | GGCC | 1 cut(s) | 763 |
| BspCNI | CTCAG | 5 cut(s) | 126, 237, 518, 721, 931 |
| BspEI | TCCGGA | 1 cut(s) | 497 |
| BspFNI | CGCG | 1 cut(s) | 611 |
| BspLI | GGNNCC | 1 cut(s) | 502 |
| BspMAI | CTGCAG | 1 cut(s) | 538 |
| BspMI | ACCTGC | 1 cut(s) | 462 |
| BspPI | GGATC | 2 cut(s) | 495, 508 |
| BsrDI | GCAATG | 1 cut(s) | 673 |
| BsrI | ACTGG | 2 cut(s) | 314, 543 |
| BssECI | CCNNGG | 3 cut(s) | 209, 301, 862 |
| BssMI | GATC | 2 cut(s) | 172, 500 |
| BssT1I | CCWWGG | 3 cut(s) | 209, 301, 862 |
| Bst2UI | CCWGG | 2 cut(s) | 765, 907 |
| Bst4CI | ACNGT | 2 cut(s) | 200, 339 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 629, 663 |
| BstC8I | GCNNGC | 1 cut(s) | 747 |
| BstDEI | CTNAG | 5 cut(s) | 113, 224, 505, 708, 918 |
| BstDSI | CCRYGG | 1 cut(s) | 209 |
| BstF5I | GGATG | 1 cut(s) | 272 |
| BstFNI | CGCG | 1 cut(s) | 611 |
| BstKTI | GATC | 2 cut(s) | 175, 503 |
| BstMAI | GTCTC | 1 cut(s) | 131 |
| BstMBI | GATC | 2 cut(s) | 172, 500 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 629, 654, 663 |
| BstNI | CCWGG | 2 cut(s) | 765, 907 |
| BstSCI | CCNGG | 2 cut(s) | 763, 905 |
| BstSFI | CTRYAG | 2 cut(s) | 534, 677 |
| BstUI | CGCG | 1 cut(s) | 611 |
| BstV1I | GCAGC | 3 cut(s) | 293, 526, 548 |
| BstX2I | RGATCY | 1 cut(s) | 500 |
| BstYI | RGATCY | 1 cut(s) | 500 |
| BsuRI | GGCC | 1 cut(s) | 763 |
| BtgI | CCRYGG | 1 cut(s) | 209 |
| BtrI | CACGTC | 1 cut(s) | 17 |
| BtsCI | GGATG | 1 cut(s) | 272 |
| BtsIMutI | CAGTG | 4 cut(s) | 321, 633, 716, 727 |
| BveI | ACCTGC | 1 cut(s) | 462 |
| Cac8I | GCNNGC | 1 cut(s) | 747 |
| CaiI | CAGNNNCTG | 1 cut(s) | 764 |
| Cfr13I | GGNCC | 1 cut(s) | 761 |
| Csp6I | GTAC | 1 cut(s) | 353 |
| CviAII | CATG | 3 cut(s) | 210, 376, 856 |
| CviQI | GTAC | 1 cut(s) | 353 |
| DdeI | CTNAG | 5 cut(s) | 113, 224, 505, 708, 918 |
| DpnI | GATC | 2 cut(s) | 174, 502 |
| DpnII | GATC | 2 cut(s) | 172, 500 |
| DraIII | CACNNNGTG | 1 cut(s) | 742 |
| Eco130I | CCWWGG | 3 cut(s) | 209, 301, 862 |
| EcoO109I | RGGNCCY | 1 cut(s) | 761 |
| EcoRI | GAATTC | 1 cut(s) | 682 |
| EcoRII | CCWGG | 2 cut(s) | 763, 905 |
| EcoT14I | CCWWGG | 3 cut(s) | 209, 301, 862 |
| EcoT22I | ATGCAT | 1 cut(s) | 934 |
| ErhI | CCWWGG | 3 cut(s) | 209, 301, 862 |
| FaeI | CATG | 3 cut(s) | 213, 379, 859 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 738, 770 |
| FaqI | GGGAC | 1 cut(s) | 325 |
| FatI | CATG | 3 cut(s) | 209, 375, 855 |
| FbaI | TGATCA | 1 cut(s) | 172 |
| FblI | GTMKAC | 1 cut(s) | 400 |
| Fnu4HI | GCNGC | 4 cut(s) | 307, 483, 537, 540 |
| FokI | GGATG | 1 cut(s) | 279 |
| Fsp4HI | GCNGC | 4 cut(s) | 307, 483, 537, 540 |
| FspBI | CTAG | 2 cut(s) | 296, 477 |
| GluI | GCNGC | 4 cut(s) | 307, 483, 537, 540 |
| HaeIII | GGCC | 1 cut(s) | 763 |
| HapII | CCGG | 1 cut(s) | 498 |
| Hin1II | CATG | 3 cut(s) | 213, 379, 859 |
| HincII | GTYRAC | 1 cut(s) | 412 |
| HindII | GTYRAC | 1 cut(s) | 412 |
| HindIII | AAGCTT | 1 cut(s) | 79 |
| HpaII | CCGG | 1 cut(s) | 498 |
| HphI | GGTGA | 2 cut(s) | 728, 818 |
| Hpy166II | GTNNAC | 5 cut(s) | 245, 401, 412, 725, 842 |
| Hpy188I | TCNGA | 3 cut(s) | 116, 227, 688 |
| Hpy188III | TCNNGA | 4 cut(s) | 65, 134, 449, 498 |
| Hpy8I | GTNNAC | 5 cut(s) | 245, 401, 412, 725, 842 |
| HpyAV | CCTTC | 2 cut(s) | 118, 323 |
| HpyCH4III | ACNGT | 2 cut(s) | 200, 339 |
| HpyCH4IV | ACGT | 2 cut(s) | 16, 838 |
| HpyCH4V | TGCA | 6 cut(s) | 536, 623, 648, 666, 745, 932 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 629, 654, 663 |
| HpyF3I | CTNAG | 5 cut(s) | 113, 224, 505, 708, 918 |
| HpySE526I | ACGT | 2 cut(s) | 16, 838 |
| Hsp92II | CATG | 3 cut(s) | 213, 379, 859 |
| Kpn2I | TCCGGA | 1 cut(s) | 497 |
| Ksp22I | TGATCA | 1 cut(s) | 172 |
| Kzo9I | GATC | 2 cut(s) | 172, 500 |
| LmnI | GCTCC | 1 cut(s) | 440 |
| Lsp1109I | GCAGC | 3 cut(s) | 293, 526, 548 |
| LweI | GCATC | 1 cut(s) | 632 |
| MaeI | CTAG | 2 cut(s) | 296, 477 |
| MaeII | ACGT | 2 cut(s) | 16, 838 |
| MaeIII | GTNAC | 3 cut(s) | 97, 255, 670 |
| MalI | GATC | 2 cut(s) | 174, 502 |
| MboI | GATC | 2 cut(s) | 172, 500 |
| MboII | GAAGA | 1 cut(s) | 3 |
| MflI | RGATCY | 1 cut(s) | 500 |
| MluCI | AATT | 6 cut(s) | 59, 71, 160, 489, 682, 876 |
| MnlI | CCTC | 9 cut(s) | 61, 122, 141, 398, 468, 505, 514, 682, 886 |
| Mph1103I | ATGCAT | 1 cut(s) | 934 |
| MroI | TCCGGA | 1 cut(s) | 497 |
| MseI | TTAA | 3 cut(s) | 576, 780, 924 |
| MslI | CAYNNNNRTG | 2 cut(s) | 380, 937 |
| MspA1I | CMGCKG | 3 cut(s) | 143, 509, 539 |
| MspI | CCGG | 1 cut(s) | 498 |
| MspR9I | CCNGG | 2 cut(s) | 765, 907 |
| MvaI | CCWGG | 2 cut(s) | 765, 907 |
| MvnI | CGCG | 1 cut(s) | 611 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 629, 654, 663 |
| NcoI | CCATGG | 1 cut(s) | 209 |
| NdeII | GATC | 2 cut(s) | 172, 500 |
| NlaIII | CATG | 3 cut(s) | 213, 379, 859 |
| NlaIV | GGNNCC | 1 cut(s) | 502 |
| NmuCI | GTSAC | 2 cut(s) | 97, 670 |
| NsiI | ATGCAT | 1 cut(s) | 934 |
| PkrI | GCNGC | 4 cut(s) | 308, 484, 538, 541 |
| Psp6I | CCWGG | 2 cut(s) | 763, 905 |
| PspGI | CCWGG | 2 cut(s) | 763, 905 |
| PspN4I | GGNNCC | 1 cut(s) | 502 |
| PspPI | GGNCC | 1 cut(s) | 761 |
| PstI | CTGCAG | 1 cut(s) | 538 |
| PstNI | CAGNNNCTG | 1 cut(s) | 764 |
| PsuI | RGATCY | 1 cut(s) | 500 |
| PvuII | CAGCTG | 3 cut(s) | 143, 509, 539 |
| RsaI | GTAC | 1 cut(s) | 354 |
| RsaNI | GTAC | 1 cut(s) | 353 |
| RseI | CAYNNNNRTG | 2 cut(s) | 380, 937 |
| SaqAI | TTAA | 3 cut(s) | 576, 780, 924 |
| SatI | GCNGC | 4 cut(s) | 307, 483, 537, 540 |
| Sau3AI | GATC | 2 cut(s) | 172, 500 |
| Sau96I | GGNCC | 1 cut(s) | 761 |
| ScrFI | CCNGG | 2 cut(s) | 765, 907 |
| SfaNI | GCATC | 1 cut(s) | 632 |
| SfcI | CTRYAG | 2 cut(s) | 534, 677 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 380, 937 |
| Sse9I | AATT | 6 cut(s) | 59, 71, 160, 489, 682, 876 |
| SsiI | CCGC | 1 cut(s) | 483 |
| SspMI | CTAG | 2 cut(s) | 296, 477 |
| StyD4I | CCNGG | 2 cut(s) | 763, 905 |
| StyI | CCWWGG | 3 cut(s) | 209, 301, 862 |
| TaaI | ACNGT | 2 cut(s) | 200, 339 |
| TaiI | ACGT | 2 cut(s) | 19, 841 |
| TaqI | TCGA | 1 cut(s) | 219 |
| TasI | AATT | 6 cut(s) | 59, 71, 160, 489, 682, 876 |
| TauI | GCSGC | 1 cut(s) | 485 |
| Tru1I | TTAA | 3 cut(s) | 576, 780, 924 |
| Tru9I | TTAA | 3 cut(s) | 576, 780, 924 |
| TscAI | CASTG | 4 cut(s) | 321, 640, 716, 727 |
| TseFI | GTSAC | 2 cut(s) | 97, 670 |
| TseI | GCWGC | 3 cut(s) | 306, 536, 539 |
| Tsp45I | GTSAC | 2 cut(s) | 97, 670 |
| TspDTI | ATGAA | 6 cut(s) | 342, 353, 528, 579, 849, 915 |
| TspRI | CASTG | 4 cut(s) | 321, 640, 716, 727 |
| XapI | RAATTY | 3 cut(s) | 59, 71, 682 |
| XmiI | GTMKAC | 1 cut(s) | 400 |
| XspI | CTAG | 2 cut(s) | 296, 477 |
| Zsp2I | ATGCAT | 1 cut(s) | 934 |