RLG00000015073

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000571
Physical Location & Seq
Forward (+)
61302564 .. 61304112
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015073

Sequence Viewer

Length: 1158 bp
ATGCCTTGCCGTCGCCATCGTCGCAGCCCTGGAGCTCCTGAAGGAACTCCTCGGCAGCGTCATTGCTTCGGTTCGAGACGTTTTCGACTCGCTGGCTCGTGCCCGAGGGCGTTTGAGTGCCCAGAGTGGCGTCGTTTTGGACTTGCTGGCAAGTTGAACAAGGCAAAGGAGTTTCTTGGGTTTATGGAGATTTTGGGGATTAAGCCTACTATAGTTACTTATAATACTATCATTCATGGGTTTTGCTTGAGAGGCAGAGTTGGAGGGGCTCAGATGATCTTTAGTGATATCAAGTTCAGCCGGATTCTTACATGTATGGATTGCTTATTAGTGGGATGTGTAAGGGGAGAAGACTTGATGAAGCGTTTGGGTGATCTGGACAGGGCATTCGGCTATAGAGATGAGATGGTGCAGAAGGGTATAATGCCAACGGTGTCAACTTACAATTTGTTGATTCATGAGTTGTTTATGGAAGGTAGGGCGAGTGAAGCCGATTGTATGGTTAGAGAAATGGAAGAGAAGGGAATGGTTCCTGATGCCATTACGTATGATATCCTGATGAATGGCTGTTGCAGGTCTGGGAATGCTAAGGAAGCATTTATCCTTCACGATGAAATGTTGAGTAAAGGGATAGAGCCCACTAAGGAAACTTATGCATCGCTTATTTATGTTTTGAGTAAAAGGAAGAGAATGAATGAGGCAGACGACTTGTTTGAGAAGATACTGTGTAAGGGCGTTCTACCGGATCTTGTGATGTTCAATGCCTTGATTGATGATCATTGTGCTAATGGGAATATGGAAAGGCACTGCAGGGCAGGGAAAGTTGAGGAAGCTCGGGAACTTCTGGATGCGATGAAGACAAGGGGAATTAAGCCTGATTACATTAGTTACAACACCCTCATTAGTGGACATAGTAAACGAGGTGATATGAATGATGCCTTCAAAGTTCGAGAGATGTTGAGCATAGGTTTCAATCCTACACTTCTAACAAACAAGGCTCTTATAAAAGGTCTATGTAAAAAGCAAGAAGGTGATCTTCCTCAAGAGCTCGTTAAAGAAATGGTGAGCAGAGGGATTACTCCCTATGACAGCACATATTACTCTCTGATTGAGGGAATTGAGAATGTTGAGGAATTTCTCAGAAAGGGCGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR_2

Protein Analysis

386

Amino Acids

43.64

Weight (kDa)

8.42

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 49 - 82 3e-06 PPR repeat family
PPR_1 PF12854 66 - 96 3.9e-10 PPR repeat
PPR_long PF17177 108 - 226 8e-09 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 143 - 192 1.6e-14 PPR repeat family
PPR_3 PF13812 168 - 227 1.3e-08 Pentatricopeptide repeat domain
TPR_24 PF23276 171 - 268 2.3e-06 Fungal tetratrico peptide repeats
PPR_1 PF12854 174 - 206 7.1e-10 PPR repeat
PPR PF01535 181 - 211 1.6e-07 PPR repeat
PPR_2 PF13041 185 - 226 7.6e-10 PPR repeat family
PPR_2 PF13041 217 - 261 2.3e-06 PPR repeat family
PPR_long PF17177 226 - 371 4.8e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 266 - 303 1.3e-09 PPR repeat family
PPR_3 PF13812 281 - 336 1.5e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 288 - 317 5.9e-08 PPR repeat
PPR_2 PF13041 292 - 340 3.8e-10 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000571)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G15630
fragaria_vesca FvH4_2g07360
malus_domestica MD02G1234600.v1.1
prunus_persica Prupe.8G019300_v2.0.a1
pyrus_communis pycom02g20240 pycom05g06440 pycom10g06900
rosa_chinensis RchiOBHm_Chr2g0106031 RchiOBHm_Chr2g0124301 RchiOBHm_Chr2g0124591 RchiOBHm_Chr5g0034701 RchiOBHm_Chr6g0257571 RchiOBHm_Chr6g0259961 RchiOBHm_Chr6g0260151 RchiOBHm_Chr6g0260161 RchiOBHm_Chr6g0260361 RchiOBHm_Chr6g0260391 RchiOBHm_Chr6g0260531 RchiOBHm_Chr6g0260541 RchiOBHm_Chr6g0260661 RchiOBHm_Chr6g0260761 RchiOBHm_Chr6g0266771 RchiOBHm_Chr7g0210581 RchiOBHm_Chr7g0225201 RchiOBHm_Chr7g0225221
rosa_laevigata RLG00000001833 RLG00000001834 RLG00000014081 RLG00000014490 RLG00000014495 RLG00000015073 RLG00000034098
rosa_multiflora Rmu_sc0000414.1_g000008 Rmu_sc0000546.1_g000067 Rmu_sc0000588.1_g000057 Rmu_sc0000861.1_g000058 Rmu_sc0000861.1_g000059 Rmu_sc0002766.1_g000023 Rmu_sc0002968.1_g000001 Rmu_sc0003786.1_g000024 Rmu_sc0005472.1_g000021 Rmu_sc0010278.1_g000006 Rmu_sc0014629.1_g000014 Rmu_sc0036189.1_g000001
rosa_roxburghii Rroxscaffold_3G00234010 Rroxscaffold_5G00368980 Rroxscaffold_7G00201460 Rroxscaffold_7G00207310 Rroxscaffold_7G00207360 Rroxscaffold_7G00207370 Rroxscaffold_7G00210730
rosa_rugosa Rorug05G0196300 Rorug05G0359600 Rorug05G0591800 Rorug05G0592300 Rorug05G0592700 Rorug06G0023500 Rorug07G0228100
rosa_samantha Rh2CG188200 Rh2DG333800 Rh4DG026900 Rh5CG249600 Rh6BG104200 Rh6BG104500 Rh6BG105900 Rh6BG106000 Rh6CG076700 Rh6CG097500 Rh6CG098200 Rh6CG100800 Rh6CG101000 Rh6CG101200 Rh6CG102400 Rh6CG144100 Rh6DG093200 Rh7AG375200 Rh7AG375300 Rh7BG360300
rosa_wichuraiana Rw0G020720 Rw5G026430 Rw6G009370 Rw6G009450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 222, 1004
Acc36I ACCTGC 1 cut(s) 564
AclWI GGATC 1 cut(s) 753
AcsI RAATTY 1 cut(s) 1133
AcuI CTGAAG 1 cut(s) 60
AcyI GRCGYC 1 cut(s) 130
AflIII ACRYGT 1 cut(s) 311
AgsI TTSAA 4 cut(s) 157, 760, 943, 973
AjnI CCWGG 1 cut(s) 28
AluBI AGCT 3 cut(s) 35, 833, 1048
AluI AGCT 3 cut(s) 35, 833, 1048
Alw21I GWGCWC 2 cut(s) 37, 1050
Alw26I GTCTC 1 cut(s) 70
AlwI GGATC 1 cut(s) 753
Ama87I CYCGRG 2 cut(s) 103, 834
ApeKI GCWGC 2 cut(s) 24, 55
ApoI RAATTY 1 cut(s) 1133
ArsI GACNNNNNNTTYG 4 cut(s) 371, 403, 695, 727
AsuHPI GGTGA 4 cut(s) 383, 935, 1043, 1075
AvaI CYCGRG 2 cut(s) 103, 834
BaeGI GKGCMC 2 cut(s) 104, 122
BanII GRGCYC 4 cut(s) 37, 271, 639, 1050
BauI CACGAG 1 cut(s) 97
BbsI GAAGAC 2 cut(s) 357, 863
Bbv12I GWGCWC 2 cut(s) 37, 1050
BbvI GCAGC 2 cut(s) 36, 67
BccI CCATC 2 cut(s) 24, 400
BciT130I CCWGG 1 cut(s) 30
BclI TGATCA 1 cut(s) 775
BcoDI GTCTC 1 cut(s) 70
BfmI CTRYAG 3 cut(s) 210, 394, 808
BfuAI ACCTGC 1 cut(s) 564
BisI GCNGC 2 cut(s) 25, 56
BlsI GCNGC 2 cut(s) 26, 57
Bme1390I CCNGG 1 cut(s) 30
BmeT110I CYCGRG 2 cut(s) 103, 834
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 1 cut(s) 30
BmsI GCATC 4 cut(s) 526, 665, 838, 925
BpiI GAAGAC 2 cut(s) 357, 863
BpmI CTGGAG 1 cut(s) 51
Bpu10I CCTNAGC 1 cut(s) 588
BpuEI CTTGAG 2 cut(s) 268, 1026
BsaAI YACGTR 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 130
BsaJI CCNNGG 3 cut(s) 28, 50, 104
BsaWI WCCGGW 1 cut(s) 742
Bse3DI GCAATG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 30
BseDI CCNNGG 3 cut(s) 28, 50, 104
BseGI GGATG 2 cut(s) 341, 853
BseMI GCAATG 1 cut(s) 61
BseMII CTCAG 2 cut(s) 284, 1153
BseRI GAGGAG 1 cut(s) 39
BseSI GKGCMC 2 cut(s) 104, 122
BseXI GCAGC 2 cut(s) 36, 67
BsgI GTGCAG 1 cut(s) 431
BsiHKAI GWGCWC 2 cut(s) 37, 1050
BsiHKCI CYCGRG 2 cut(s) 103, 834
BsiSI CCGG 2 cut(s) 301, 743
BsmAI GTCTC 1 cut(s) 70
BsmBI CGTCTC 1 cut(s) 70
BsmI GAATGC 2 cut(s) 386, 589
BsoBI CYCGRG 2 cut(s) 103, 834
Bsp1286I GDGCHC 6 cut(s) 37, 104, 122, 271, 639, 1050
Bsp143I GATC 5 cut(s) 276, 373, 745, 775, 1033
BspCNI CTCAG 2 cut(s) 283, 1152
BspHI TCATGA 2 cut(s) 457, 1154
BspLI GGNNCC 1 cut(s) 531
BspMAI CTGCAG 1 cut(s) 812
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 1 cut(s) 753
BsrDI GCAATG 1 cut(s) 61
BssECI CCNNGG 3 cut(s) 28, 50, 104
BssMI GATC 5 cut(s) 276, 373, 745, 775, 1033
BssNI GRCGYC 1 cut(s) 130
BssSI CACGAG 1 cut(s) 97
Bst2BI CACGAG 1 cut(s) 97
Bst2UI CCWGG 1 cut(s) 30
Bst4CI ACNGT 2 cut(s) 433, 726
Bst6I CTCTTC 2 cut(s) 510, 680
BstACI GRCGYC 1 cut(s) 130
BstBAI YACGTR 1 cut(s) 546
BstC8I GCNNGC 2 cut(s) 94, 148
BstDEI CTNAG 4 cut(s) 270, 588, 642, 1139
BstF5I GGATG 2 cut(s) 341, 853
BstKTI GATC 5 cut(s) 279, 376, 748, 778, 1036
BstMAI GTCTC 1 cut(s) 70
BstMBI GATC 5 cut(s) 276, 373, 745, 775, 1033
BstMWI GCNNNNNNNGC 4 cut(s) 21, 252, 488, 593
BstNI CCWGG 1 cut(s) 30
BstNSI RCATGY 1 cut(s) 315
BstSCI CCNGG 1 cut(s) 28
BstSFI CTRYAG 3 cut(s) 210, 394, 808
BstSLI GKGCMC 2 cut(s) 104, 122
BstSNI TACGTA 1 cut(s) 546
BstV1I GCAGC 2 cut(s) 36, 67
BstV2I GAAGAC 2 cut(s) 357, 863
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BtgZI GCGATG 2 cut(s) 642, 866
BtsCI GGATG 2 cut(s) 341, 853
BtsI GCAGTG 1 cut(s) 805
BtsIMutI CAGTG 1 cut(s) 805
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 2 cut(s) 94, 148
CciI TCATGA 2 cut(s) 457, 1154
CseI GACGC 2 cut(s) 47, 119
CviAII CATG 4 cut(s) 236, 312, 458, 1155
DdeI CTNAG 4 cut(s) 270, 588, 642, 1139
DpnI GATC 5 cut(s) 278, 375, 747, 777, 1035
DpnII GATC 5 cut(s) 276, 373, 745, 775, 1033
Eam1104I CTCTTC 2 cut(s) 510, 680
EarI CTCTTC 2 cut(s) 510, 680
Ecl136II GAGCTC 2 cut(s) 35, 1048
Eco105I TACGTA 1 cut(s) 546
Eco24I GRGCYC 4 cut(s) 37, 271, 639, 1050
Eco32I GATATC 2 cut(s) 289, 553
Eco53kI GAGCTC 2 cut(s) 35, 1048
Eco57I CTGAAG 1 cut(s) 60
Eco88I CYCGRG 2 cut(s) 103, 834
EcoICRI GAGCTC 2 cut(s) 35, 1048
EcoRII CCWGG 1 cut(s) 28
EcoRV GATATC 2 cut(s) 289, 553
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 4 cut(s) 37, 271, 639, 1050
Esp3I CGTCTC 1 cut(s) 70
FaeI CATG 4 cut(s) 239, 315, 461, 1158
FalI AAGNNNNNCTT 2 cut(s) 1020, 1052
FatI CATG 4 cut(s) 235, 311, 457, 1154
FbaI TGATCA 1 cut(s) 775
Fnu4HI GCNGC 2 cut(s) 25, 56
FokI GGATG 2 cut(s) 348, 860
FriOI GRGCYC 4 cut(s) 37, 271, 639, 1050
Fsp4HI GCNGC 2 cut(s) 25, 56
GluI GCNGC 2 cut(s) 25, 56
GsuI CTGGAG 1 cut(s) 51
HapII CCGG 2 cut(s) 301, 743
HgaI GACGC 2 cut(s) 47, 119
Hin1I GRCGYC 1 cut(s) 130
Hin1II CATG 4 cut(s) 239, 315, 461, 1158
HincII GTYRAC 1 cut(s) 438
HindII GTYRAC 1 cut(s) 438
HinfI GANTC 4 cut(s) 87, 304, 454, 1151
HpaII CCGG 2 cut(s) 301, 743
HphI GGTGA 4 cut(s) 383, 935, 1043, 1075
Hpy166II GTNNAC 3 cut(s) 438, 908, 917
Hpy188I TCNGA 3 cut(s) 273, 1107, 1142
Hpy8I GTNNAC 3 cut(s) 438, 908, 917
Hpy99I CGWCG 3 cut(s) 15, 24, 135
HpyAV CCTTC 7 cut(s) 35, 409, 467, 514, 614, 949, 1022
HpyCH4III ACNGT 2 cut(s) 433, 726
HpyCH4IV ACGT 2 cut(s) 79, 545
HpyCH4V TGCA 4 cut(s) 412, 573, 656, 810
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 252, 488, 593
HpyF3I CTNAG 4 cut(s) 270, 588, 642, 1139
HpySE526I ACGT 2 cut(s) 79, 545
Hsp92I GRCGYC 1 cut(s) 130
Hsp92II CATG 4 cut(s) 239, 315, 461, 1158
Ksp22I TGATCA 1 cut(s) 775
Kzo9I GATC 5 cut(s) 276, 373, 745, 775, 1033
LmnI GCTCC 2 cut(s) 32, 40
Lsp1109I GCAGC 2 cut(s) 36, 67
LweI GCATC 4 cut(s) 526, 665, 838, 925
MaeII ACGT 2 cut(s) 79, 545
MaeIII GTNAC 2 cut(s) 214, 887
MalI GATC 5 cut(s) 278, 375, 747, 777, 1035
MboI GATC 5 cut(s) 276, 373, 745, 775, 1033
MboII GAAGA 6 cut(s) 362, 527, 697, 730, 868, 1028
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 6 cut(s) 37, 104, 122, 271, 639, 1050
MluCI AATT 4 cut(s) 445, 867, 1116, 1133
MlyI GAGTC 1 cut(s) 81
MmeI TCCRAC 1 cut(s) 241
Mph1103I ATGCAT 1 cut(s) 658
MseI TTAA 3 cut(s) 201, 870, 1053
MspI CCGG 2 cut(s) 301, 743
MspR9I CCNGG 1 cut(s) 30
Mva1269I GAATGC 2 cut(s) 386, 589
MvaI CCWGG 1 cut(s) 30
MwoI GCNNNNNNNGC 4 cut(s) 21, 252, 488, 593
NdeII GATC 5 cut(s) 276, 373, 745, 775, 1033
NlaIII CATG 4 cut(s) 239, 315, 461, 1158
NlaIV GGNNCC 1 cut(s) 531
NmeAIII GCCGAG 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 315
PagI TCATGA 2 cut(s) 457, 1154
PciI ACATGT 1 cut(s) 311
PctI GAATGC 2 cut(s) 386, 589
PfeI GAWTC 3 cut(s) 304, 454, 1151
PkrI GCNGC 2 cut(s) 26, 57
PleI GAGTC 1 cut(s) 81
PpsI GAGTC 1 cut(s) 81
Ppu21I YACGTR 1 cut(s) 546
PscI ACATGT 1 cut(s) 311
PsiI TTATAA 2 cut(s) 222, 1004
Psp124BI GAGCTC 2 cut(s) 37, 1050
Psp6I CCWGG 1 cut(s) 28
PspGI CCWGG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 531
PstI CTGCAG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 745
SacI GAGCTC 2 cut(s) 37, 1050
SaqAI TTAA 3 cut(s) 201, 870, 1053
SatI GCNGC 2 cut(s) 25, 56
Sau3AI GATC 5 cut(s) 276, 373, 745, 775, 1033
SchI GAGTC 1 cut(s) 81
ScrFI CCNGG 1 cut(s) 30
SduI GDGCHC 6 cut(s) 37, 104, 122, 271, 639, 1050
SfaNI GCATC 4 cut(s) 526, 665, 838, 925
SfcI CTRYAG 3 cut(s) 210, 394, 808
SmlI CTYRAG 2 cut(s) 247, 1041
SmoI CTYRAG 2 cut(s) 247, 1041
SnaBI TACGTA 1 cut(s) 546
Sse9I AATT 4 cut(s) 445, 867, 1116, 1133
SstI GAGCTC 2 cut(s) 37, 1050
StyD4I CCNGG 1 cut(s) 28
TaaI ACNGT 2 cut(s) 433, 726
TaiI ACGT 2 cut(s) 82, 548
TaqI TCGA 3 cut(s) 74, 85, 949
TasI AATT 4 cut(s) 445, 867, 1116, 1133
TfiI GAWTC 3 cut(s) 304, 454, 1151
Tru1I TTAA 3 cut(s) 201, 870, 1053
Tru9I TTAA 3 cut(s) 201, 870, 1053
TscAI CASTG 1 cut(s) 812
TseI GCWGC 2 cut(s) 24, 55
TspDTI ATGAA 9 cut(s) 224, 374, 446, 575, 627, 707, 869, 944, 1143
TspRI CASTG 1 cut(s) 812
XapI RAATTY 1 cut(s) 1133
XceI RCATGY 1 cut(s) 315
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.