Rmu_sc0000424.1_g000031

fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000424.1
OG0000614
Physical Location & Seq
Reverse (-)
125870 .. 127015
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000424.1_g000031.1.cds

Sequence Viewer

Length: 1146 bp
atggcttcttcttcttcttcaacctcactcttcctcttcttctctctcctcctcctcctctctctcctctccttctcctccgcccaaaccatcaccaacgccgccgagatcctctccaattccggctacaaatccatgtccctcgccctcgacctcgcctctcactccatcacctcccagtcgccgtctctcacagtcttcgctccggccgacgccgccttcgccaactccggccaccccactctcaacctcctccgctaccacctcctccccatcgctttccctctccgatcactcaagtccctcccgttcggcgccaagatcgccactctgctcgacggccactctctcaccgtcaccactcttccggccgaccgccttgtctcgctcaacaacgtcaccgtcacggcggccccaattttcgacgacgggtcgttgatcgtcttcggaaccagcggattcttcgaccggtatttccggatctcgggtccgattgggagctacagccccaagaatctcggctgcttggcgccgcgaaaccctaacagagcgatggcggtggaaacggcggcgtattacccgttcagtgaagcaagcaaggtgctgagatcgaaaggctgctctgtcatggcttcgtttcttgaaatgcaattccatgggtttctcaagtaccagaccatgttgaccgtcttggctcccgttgaccaagtcatggtgaagcggatcggaaatctgagcgagtactcgtcgatcttgcgccgccacgtggtcccctgccggctttctgggagcgatttgaagagtcttgaggatgggactgtgttgaggactaatttgcaagggtttatgatcaacgtgagtcgatacgacaccgtttttgtgctgaatggggtcccggtgattatgccggagctgtatcacaacggctggcttgtggttcatggaatcagtgaggttcttgaacttccagtgagcctaactcagagcccaattcagagtcaaacggagccagtggtggatacggtcgatccggtggcttcttcacctccctctccggcacttcatcgcagctccggtgaggttctttgtttgccggccttcataatctgggtacctcttctgtttttctatgtttttaggccttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Fasciclin

Protein Analysis

381

Amino Acids

41.34

Weight (kDa)

7.74

Isoelectric Point (pI)

52.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000614)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40940
fragaria_vesca FvH4_2g32760 FvH4_4g32150 FvH4_4g32590 FvH4_6g20740 FvH4_6g53540 FvH4_7g12190
malus_domestica MD09G1010600.v1.1 MD13G1026900.v1.1 MD13G1028200.v1.1 MD16G1030000.v1.1 MD16G1031100.v1.1
prunus_persica Prupe.1G322000_v2.0.a1 Prupe.3G306500_v2.0.a1
pyrus_communis pycom01g06180 pycom04g10020 pycom111g00880 pycom13g02560 pycom13g02660 pycom16g02710
rosa_chinensis RchiOBHm_Chr2g0175581 RchiOBHm_Chr4g0440841
rosa_laevigata RLG00000006133 RLG00000006134 RLG00000006144 RLG00000008208 RLG00000019874 RLG00000022341 RLG00000032925 RLG00000036410
rosa_multiflora Rmu_co8113152.1_g000001 Rmu_sc0000424.1_g000015 Rmu_sc0000424.1_g000029 Rmu_sc0000424.1_g000030 Rmu_sc0000424.1_g000031 Rmu_sc0000998.1_g000023 Rmu_sc0005054.1_g000012 Rmu_sc0009386.1_g000004 Rmu_sc0013970.1_g000002 Rmu_sc0027146.1_g000001 Rmu_ssc0000175.1_g000015 Rmu_ssc0000386.1_g000026
rosa_roxburghii Rroxscaffold_2G00077200 Rroxscaffold_5G00381670 Rroxscaffold_5G00381840 Rroxscaffold_5G00381850
rosa_rugosa Rorug03G0261000 Rorug04G0120500 Rorug04G0328900 Rorug04G0329900 Rorug04G0330000 Rorug05G0525900
rosa_samantha Rh1AG187300 Rh1BG153500 Rh1CG172800 Rh2BG270100 Rh2BG676400 Rh2CG639600 Rh2DG690400 Rh3BG272200 Rh4AG380600 Rh4AG381600 Rh4AG381700 Rh4BG393200 Rh4BG395000 Rh4BG395100 Rh4CG053800 Rh4CG194900 Rh4CG408000 Rh4CG409900 Rh4CG410000 Rh5BG280900 Rh7AG473300 Rh7BG247300 Rh7CG108400
rosa_wichuraiana Rw2G054570 Rw4G032740 Rw4G032880 Rw4G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 380
Acc65I GGTACC 1 cut(s) 1111
AccB1I GGYRCC 3 cut(s) 314, 529, 1111
AccB7I CCANNNNNTGG 1 cut(s) 712
AccII CGCG 1 cut(s) 535
AccIII TCCGGA 1 cut(s) 477
AclWI GGATC 4 cut(s) 103, 488, 731, 1022
AcoI YGGCCR 4 cut(s) 207, 232, 340, 369
AcvI CACGTG 1 cut(s) 766
AcyI GRCGYC 3 cut(s) 213, 315, 530
AfaI GTAC 3 cut(s) 671, 743, 1113
AfiI CCNNNNNNNGG 3 cut(s) 484, 712, 766
AgeI ACCGGT 1 cut(s) 468
AgsI TTSAA 4 cut(s) 21, 644, 799, 962
AhdI GACNNNNNGTC 1 cut(s) 430
AluBI AGCT 3 cut(s) 501, 913, 1071
AluI AGCT 3 cut(s) 501, 913, 1071
Alw26I GTCTC 2 cut(s) 192, 388
AlwI GGATC 4 cut(s) 103, 488, 731, 1022
Ama87I CYCGRG 1 cut(s) 484
Aor13HI TCCGGA 1 cut(s) 477
AoxI GGCC 7 cut(s) 207, 232, 340, 369, 411, 1095, 1139
ApeKI GCWGC 3 cut(s) 522, 618, 1068
ArsI GACNNNNNNTTYG 4 cut(s) 203, 235, 860, 892
AsiGI ACCGGT 1 cut(s) 468
Asp718I GGTACC 1 cut(s) 1111
AspLEI GCGC 3 cut(s) 317, 532, 759
AspS9I GGNCC 4 cut(s) 412, 488, 769, 892
AsuC2I CCSGG 1 cut(s) 896
AsuHPI GGTGA 9 cut(s) 85, 163, 343, 349, 391, 727, 910, 1035, 1088
AvaI CYCGRG 1 cut(s) 484
AvaII GGWCC 3 cut(s) 488, 769, 892
BanI GGYRCC 3 cut(s) 314, 529, 1111
BanII GRGCYC 1 cut(s) 989
BbrPI CACGTG 1 cut(s) 766
BbsI GAAGAC 2 cut(s) 190, 436
BbvI GCAGC 3 cut(s) 509, 605, 1080
BccI CCATC 5 cut(s) 98, 176, 281, 547, 806
BceAI ACGGC 5 cut(s) 169, 355, 423, 582, 940
BciVI GTATCC 1 cut(s) 1012
BclI TGATCA 1 cut(s) 849
BcnI CCSGG 1 cut(s) 896
BcoDI GTCTC 2 cut(s) 192, 388
BfmI CTRYAG 1 cut(s) 502
BfoI RGCGCY 2 cut(s) 318, 533
BfuI GTATCC 1 cut(s) 1012
BisI GCNGC 9 cut(s) 102, 216, 411, 523, 533, 570, 619, 760, 1069
BlsI GCNGC 9 cut(s) 103, 217, 412, 524, 534, 571, 620, 761, 1070
BmcAI AGTACT 1 cut(s) 743
Bme1390I CCNGG 1 cut(s) 896
Bme18I GGWCC 3 cut(s) 488, 769, 892
BmeRI GACNNNNNGTC 1 cut(s) 430
BmeT110I CYCGRG 1 cut(s) 484
BmgT120I GGNCC 4 cut(s) 412, 488, 769, 892
BmrFI CCNGG 1 cut(s) 896
BmrI ACTGGG 1 cut(s) 172
BmuI ACTGGG 1 cut(s) 172
BpiI GAAGAC 2 cut(s) 190, 436
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 964, 996
BpuEI CTTGAG 3 cut(s) 281, 650, 827
BpuMI CCSGG 1 cut(s) 896
BsaAI YACGTR 1 cut(s) 766
BsaHI GRCGYC 3 cut(s) 213, 315, 530
BsaJI CCNNGG 1 cut(s) 655
BsaWI WCCGGW 4 cut(s) 468, 477, 1030, 1073
Bsc4I CCNNNNNNNGG 3 cut(s) 484, 712, 766
Bse118I RCCGGY 3 cut(s) 468, 777, 1093
Bse1I ACTGG 3 cut(s) 178, 968, 1010
BseAI TCCGGA 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 655
BseGI GGATG 1 cut(s) 817
BseLI CCNNNNNNNGG 3 cut(s) 484, 712, 766
BseMII CTCAG 3 cut(s) 596, 725, 995
BseNI ACTGG 3 cut(s) 178, 968, 1010
BseRI GAGGAG 8 cut(s) 38, 41, 44, 47, 56, 67, 242, 257
BseX3I CGGCCG 2 cut(s) 207, 369
BseXI GCAGC 3 cut(s) 509, 605, 1080
Bsh1236I CGCG 1 cut(s) 535
Bsh1285I CGRYCG 5 cut(s) 210, 372, 376, 469, 1026
BshFI GGCC 7 cut(s) 209, 234, 342, 371, 413, 1097, 1141
BshNI GGYRCC 3 cut(s) 314, 529, 1111
BshTI ACCGGT 1 cut(s) 468
BsiEI CGRYCG 5 cut(s) 210, 372, 376, 469, 1026
BsiHKCI CYCGRG 1 cut(s) 484
BslFI GGGAC 5 cut(s) 124, 286, 755, 829, 878
BslI CCNNNNNNNGG 3 cut(s) 484, 712, 766
BsmAI GTCTC 2 cut(s) 192, 388
BsmBI CGTCTC 1 cut(s) 192
BsmFI GGGAC 5 cut(s) 124, 286, 755, 829, 878
BsnI GGCC 7 cut(s) 209, 234, 342, 371, 413, 1097, 1141
BsoBI CYCGRG 1 cut(s) 484
Bsp1286I GDGCHC 1 cut(s) 989
Bsp13I TCCGGA 1 cut(s) 477
Bsp19I CCATGG 1 cut(s) 655
BspANI GGCC 7 cut(s) 209, 234, 342, 371, 413, 1097, 1141
BspCNI CTCAG 3 cut(s) 597, 726, 994
BspEI TCCGGA 1 cut(s) 477
BspFNI CGCG 1 cut(s) 535
BspPI GGATC 4 cut(s) 103, 488, 731, 1022
BspT107I GGYRCC 3 cut(s) 314, 529, 1111
BsrFI RCCGGY 3 cut(s) 468, 777, 1093
BsrI ACTGG 3 cut(s) 178, 968, 1010
BssAI RCCGGY 3 cut(s) 468, 777, 1093
BssECI CCNNGG 1 cut(s) 655
BssNI GRCGYC 3 cut(s) 213, 315, 530
BssT1I CCWWGG 1 cut(s) 655
Bst4CI ACNGT 7 cut(s) 196, 355, 403, 688, 820, 874, 1024
Bst6I CTCTTC 5 cut(s) 35, 41, 369, 794, 1122
BstACI GRCGYC 3 cut(s) 213, 315, 530
BstBAI YACGTR 1 cut(s) 766
BstC8I GCNNGC 4 cut(s) 595, 779, 929, 1095
BstDEI CTNAG 3 cut(s) 605, 734, 981
BstDSI CCRYGG 1 cut(s) 655
BstF5I GGATG 1 cut(s) 817
BstFNI CGCG 1 cut(s) 535
BstH2I RGCGCY 2 cut(s) 318, 533
BstHHI GCGC 3 cut(s) 317, 532, 759
BstMAI GTCTC 2 cut(s) 192, 388
BstMCI CGRYCG 5 cut(s) 210, 372, 376, 469, 1026
BstMWI GCNNNNNNNGC 3 cut(s) 215, 221, 323
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 1 cut(s) 502
BstUI CGCG 1 cut(s) 535
BstV1I GCAGC 3 cut(s) 509, 605, 1080
BstV2I GAAGAC 2 cut(s) 190, 436
BstX2I RGATCY 2 cut(s) 108, 480
BstYI RGATCY 2 cut(s) 108, 480
BstZI CGGCCG 2 cut(s) 207, 369
BsuI GTATCC 1 cut(s) 1012
BsuRI GGCC 7 cut(s) 209, 234, 342, 371, 413, 1097, 1141
BtgI CCRYGG 1 cut(s) 655
BtgZI GCGATG 3 cut(s) 259, 566, 1049
BtsCI GGATG 1 cut(s) 817
BtsIMutI CAGTG 4 cut(s) 592, 955, 975, 1017
Cac8I GCNNGC 4 cut(s) 595, 779, 929, 1095
CfoI GCGC 3 cut(s) 317, 532, 759
Cfr10I RCCGGY 3 cut(s) 468, 777, 1093
Cfr13I GGNCC 4 cut(s) 412, 488, 769, 892
CseI GACGC 1 cut(s) 221
Csp6I GTAC 3 cut(s) 670, 742, 1112
CspAI ACCGGT 1 cut(s) 468
CviAII CATG 6 cut(s) 136, 628, 656, 679, 712, 941
CviQI GTAC 3 cut(s) 670, 742, 1112
DdeI CTNAG 3 cut(s) 605, 734, 981
DinI GGCGCC 2 cut(s) 316, 531
DrdI GACNNNNNNGTC 1 cut(s) 380
DriI GACNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 380
EaeI YGGCCR 4 cut(s) 207, 232, 340, 369
EagI CGGCCG 2 cut(s) 207, 369
Eam1104I CTCTTC 5 cut(s) 35, 41, 369, 794, 1122
Eam1105I GACNNNNNGTC 1 cut(s) 430
EarI CTCTTC 5 cut(s) 35, 41, 369, 794, 1122
EciI GGCGGA 1 cut(s) 70
EclXI CGGCCG 2 cut(s) 207, 369
Eco130I CCWWGG 1 cut(s) 655
Eco147I AGGCCT 1 cut(s) 1141
Eco24I GRGCYC 1 cut(s) 989
Eco47I GGWCC 3 cut(s) 488, 769, 892
Eco52I CGGCCG 2 cut(s) 207, 369
Eco72I CACGTG 1 cut(s) 766
Eco88I CYCGRG 1 cut(s) 484
EcoO109I RGGNCCY 1 cut(s) 892
EcoT14I CCWWGG 1 cut(s) 655
EcoT38I GRGCYC 1 cut(s) 989
EgeI GGCGCC 2 cut(s) 316, 531
EheI GGCGCC 2 cut(s) 316, 531
ErhI CCWWGG 1 cut(s) 655
Esp3I CGTCTC 1 cut(s) 192
FaeI CATG 6 cut(s) 139, 631, 659, 682, 715, 944
FaqI GGGAC 5 cut(s) 124, 286, 755, 829, 878
FatI CATG 6 cut(s) 135, 627, 655, 678, 711, 940
FbaI TGATCA 1 cut(s) 849
Fnu4HI GCNGC 9 cut(s) 102, 216, 411, 523, 533, 570, 619, 760, 1069
FokI GGATG 1 cut(s) 824
FriOI GRGCYC 1 cut(s) 989
Fsp4HI GCNGC 9 cut(s) 102, 216, 411, 523, 533, 570, 619, 760, 1069
GlaI GCGC 3 cut(s) 316, 531, 758
GluI GCNGC 9 cut(s) 102, 216, 411, 523, 533, 570, 619, 760, 1069
HaeII RGCGCY 2 cut(s) 318, 533
HaeIII GGCC 7 cut(s) 209, 234, 342, 371, 413, 1097, 1141
HgaI GACGC 1 cut(s) 221
HhaI GCGC 3 cut(s) 317, 532, 759
Hin1I GRCGYC 3 cut(s) 213, 315, 530
Hin1II CATG 6 cut(s) 139, 631, 659, 682, 715, 944
Hin6I GCGC 3 cut(s) 315, 530, 757
HinP1I GCGC 3 cut(s) 315, 530, 757
HincII GTYRAC 2 cut(s) 684, 703
HindII GTYRAC 2 cut(s) 684, 703
HinfI GANTC 6 cut(s) 459, 514, 802, 859, 945, 997
HphI GGTGA 9 cut(s) 85, 163, 343, 349, 391, 727, 910, 1035, 1088
Hpy166II GTNNAC 2 cut(s) 684, 703
Hpy188I TCNGA 7 cut(s) 290, 449, 492, 728, 735, 984, 996
Hpy188III TCNNGA 4 cut(s) 478, 641, 806, 959
Hpy8I GTNNAC 2 cut(s) 684, 703
Hpy99I CGWCG 5 cut(s) 215, 341, 428, 431, 751
HpyAV CCTTC 3 cut(s) 82, 229, 1108
HpyCH4III ACNGT 7 cut(s) 196, 355, 403, 688, 820, 874, 1024
HpyCH4IV ACGT 3 cut(s) 396, 765, 855
HpyCH4V TGCA 2 cut(s) 649, 838
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 221, 323
HpyF3I CTNAG 3 cut(s) 605, 734, 981
HpySE526I ACGT 3 cut(s) 396, 765, 855
Hsp92I GRCGYC 3 cut(s) 213, 315, 530
Hsp92II CATG 6 cut(s) 139, 631, 659, 682, 715, 944
HspAI GCGC 3 cut(s) 315, 530, 757
KasI GGCGCC 2 cut(s) 314, 529
KflI GGGWCCC 1 cut(s) 892
Kpn2I TCCGGA 1 cut(s) 477
KpnI GGTACC 1 cut(s) 1115
KroI GCCGGC 2 cut(s) 777, 1093
KroNI GCCGGC 2 cut(s) 779, 1095
Ksp22I TGATCA 1 cut(s) 849
LmnI GCTCC 7 cut(s) 208, 498, 700, 789, 910, 1006, 1076
Lsp1109I GCAGC 3 cut(s) 509, 605, 1080
MaeII ACGT 3 cut(s) 396, 765, 855
MaeIII GTNAC 3 cut(s) 355, 397, 403
MflI RGATCY 2 cut(s) 108, 480
MhlI GDGCHC 1 cut(s) 989
MluCI AATT 5 cut(s) 118, 417, 650, 832, 990
Mly113I GGCGCC 2 cut(s) 315, 530
MlyI GAGTC 3 cut(s) 811, 868, 1006
MroI TCCGGA 1 cut(s) 477
MroNI GCCGGC 2 cut(s) 777, 1093
MspA1I CMGCKG 1 cut(s) 456
MspR9I CCNGG 1 cut(s) 896
MvnI CGCG 1 cut(s) 535
MwoI GCNNNNNNNGC 3 cut(s) 215, 221, 323
NaeI GCCGGC 2 cut(s) 779, 1095
NarI GGCGCC 2 cut(s) 315, 530
NciI CCSGG 1 cut(s) 896
NcoI CCATGG 1 cut(s) 655
NgoMIV GCCGGC 2 cut(s) 777, 1093
NlaIII CATG 6 cut(s) 139, 631, 659, 682, 715, 944
NmeAIII GCCGAG 2 cut(s) 130, 498
NmuCI GTSAC 3 cut(s) 355, 397, 403
PceI AGGCCT 1 cut(s) 1141
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdiI GCCGGC 2 cut(s) 779, 1095
PfeI GAWTC 3 cut(s) 459, 514, 945
PflFI GACNNNGTC 1 cut(s) 707
PflMI CCANNNNNTGG 1 cut(s) 712
PinAI ACCGGT 1 cut(s) 468
PkrI GCNGC 9 cut(s) 103, 217, 412, 524, 534, 571, 620, 761, 1070
PleI GAGTC 3 cut(s) 810, 867, 1005
PluTI GGCGCC 2 cut(s) 318, 533
PmaCI CACGTG 1 cut(s) 766
PmlI CACGTG 1 cut(s) 766
PpsI GAGTC 3 cut(s) 810, 867, 1005
Ppu21I YACGTR 1 cut(s) 766
PpuMI RGGWCCY 1 cut(s) 892
Psp5II RGGWCCY 1 cut(s) 892
PspCI CACGTG 1 cut(s) 766
PspPI GGNCC 4 cut(s) 412, 488, 769, 892
PspPPI RGGWCCY 1 cut(s) 892
PsrI GAACNNNNNNTAC 2 cut(s) 566, 598
PsuI RGATCY 2 cut(s) 108, 480
PsyI GACNNNGTC 1 cut(s) 707
RsaI GTAC 3 cut(s) 671, 743, 1113
RsaNI GTAC 3 cut(s) 670, 742, 1112
SatI GCNGC 9 cut(s) 102, 216, 411, 523, 533, 570, 619, 760, 1069
Sau96I GGNCC 4 cut(s) 412, 488, 769, 892
ScaI AGTACT 1 cut(s) 743
SchI GAGTC 3 cut(s) 811, 868, 1006
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 1 cut(s) 989
SfcI CTRYAG 1 cut(s) 502
SfoI GGCGCC 2 cut(s) 316, 531
SinI GGWCC 3 cut(s) 488, 769, 892
SmlI CTYRAG 3 cut(s) 296, 665, 806
SmoI CTYRAG 3 cut(s) 296, 665, 806
Sse9I AATT 5 cut(s) 118, 417, 650, 832, 990
SseBI AGGCCT 1 cut(s) 1141
SspDI GGCGCC 2 cut(s) 314, 529
StuI AGGCCT 1 cut(s) 1141
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 7 cut(s) 196, 355, 403, 688, 820, 874, 1024
TaiI ACGT 3 cut(s) 399, 768, 858
TaqI TCGA 8 cut(s) 150, 336, 423, 465, 611, 749, 862, 1026
TasI AATT 5 cut(s) 118, 417, 650, 832, 990
TatI WGTACW 1 cut(s) 741
TauI GCSGC 6 cut(s) 104, 218, 413, 535, 572, 762
TfiI GAWTC 3 cut(s) 459, 514, 945
TscAI CASTG 4 cut(s) 592, 955, 975, 1017
TseFI GTSAC 3 cut(s) 355, 397, 403
TseI GCWGC 3 cut(s) 522, 618, 1068
Tsp45I GTSAC 3 cut(s) 355, 397, 403
TspDTI ATGAA 3 cut(s) 929, 1052, 1090
TspGWI ACGGA 1 cut(s) 1019
TspRI CASTG 4 cut(s) 592, 955, 975, 1017
Tth111I GACNNNGTC 1 cut(s) 707
Van91I CCANNNNNTGG 1 cut(s) 712
VpaK11BI GGWCC 3 cut(s) 488, 769, 892
ZrmI AGTACT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.