Rorug03G0261000

Pentatricopeptide repeat-containing protein At1g77360, mitochondrial-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000614
Physical Location & Seq
Forward (+)
25113938 .. 25128989
15052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0261000.1

Sequence Viewer

Length: 2901 bp
ATGGCTAGCTCTACAAAAGCTATGGATCCAGCTTTTCAGGGAGCAGGTCAAAGGGTAGGGACTGAAATTTGGCGAATTGAGGATTTCCAGCCAGTTCCGTTGCCAAAATCTGAATATGGAAAATTCTATATGGGAGATTCTTATATTGTCTTGCAGACAACACAGAACAGAGGGGGTGCTTATCTCTATGACATCCACTTCTGGATTGGGAAAGATACAAGTCAGGATGAAGCTGGCACAGCAGCTATTAAAACTGTTGAACTAGATACAGCTCTTGGAGGACGTGCTGTGCAACATAGGGAAATTCAAGGCCATGAATCTGATAAGTTTTTGTCCTATTTCAAACCATGTATTATACCTCTGGAGGGGGGTGTTGCATCTGGATTTAAAAAGCCTGAGGAAGAAGAATTTGAAACTCGACTTTATATCTGCAGAGGAAAACGTGTTGTCAGAATGAAGCAGGTTCCTTTTGCGCGTTCGTCACTGAATCATGATGATGTGTTCATCCTGGACAGCCAGAATAAGATCTTTCAGTTCAACGGTGCGAATTCCAATATCCAGGAAAGGGCGAAGGCTTTGGAAGTCATCCAATTTTTGAAAGAAAAGTATCATCATGGGACATGTGATGTTGCAATAGTTGATGATGGGAAGCTAGACACAGAATCTGACTCTGGTGAGTTCTGGGTCCTAATGGGTGGCTTTGCTCCGATTAGCAAGAAGGTTGCCAATGAAGATGATGTCGTTCCTGAGTCCACTCCTGCTACACTGTATAGCATAACTGATGGTGAAGTGAAGATCGTAGAAGGTGAACTATCCAAATCCTTGCTTGAAAACAACAAATGCTACTTACTGGACTGTGGTTCTGAAGTATTTGTTTGGGTCGGTCGGGTGACACAAGTGGAGGACAGAAAAGCTGCTAGCCAAGCAGCTGAGGAATTTGTTTGTCATCAGAATAGACCAAAATCAACACGAATAACCCGGCTTATTCAAGGTTATGAGACACGTTCATTCAAGTCCAACTTCGATTCTTGGCCATTGGGATCAGCAACGTCTGGTGCTGAGGAGGGAAGAGGAAAAGTAGCAGCTTTGCTGAAGCAACAAGGTGTTGGTGTAAAGGGCGCGACAAAGGGTGCCCCTGTAAATGAAGAGGTCCCACCTTTGCTTGAAGGAGGTGGAAAGATGGAGGTATGGTGCATCAATGGCAGCGCTAAAACCCAATTACCTAAGGAAGATATCGGCAAATTTTTTAGTGGAGATTGCTACATAATTCTTTATACGTACCACTCTGGTGATAGAAAGGATGACTACTTTCTGTGCTGTTGGTTTGGAAAGGATAGTATTGAGGAGGACCAGAAAACAGCTTCTCATTTGGCGACTTCAATTTCCAACTCACTGAAGGGAAGACCGGTTCAGGGTCACATATTCCAAGGTAAAGAGCCACCACAGTTTGTTGCGCTCTTTCAGCCTATGGTTGTTCTCAAGGGTGGCCTGAGCTCTGGGTATAAAAAGTTTGTTGAGGAGAAAGGGTTGGCTGATGAAACTTACACAGCAGAGTGTGTTGCACTCTTTCGGTTATCTGGAACCTCTATTCATAATAACAAAGCCGTGCAAGTTGATACGGTGGCAACGTCATTGAACTCTACCGAGTGTTTTCTTCTGCAATCTGGCTCCTCAGTTTTTGCTTGGAATGGAAATCAATCTACCATTGAGCAGCAACAGTTAGCAGCAAAGCTTGCAGAATTTCTGAAGCCAGGAGTTACTTTAAAACATGCTAAAGAAGGAACTGAGAGCTCAACCTTCTGGTCTGCCCTAGGAGGGAAACAAAATTACACCAGTAATAAAGTTGCTTCTGAGATTTGCAGAGATCCACACCTGTTCACATTCTCATTCAACAAAGGAAAGTTTCAGGTGGAAGAAATATACAACTTTACTCAAGATGATCTACTGACAGAGGATATCCTTATACTGGATACACATGCTGAAGTCTTTGTTTGGGTTGGTCAATGTGTAGACTTGAAAGAAAAACAAAATGCTTTTGAAATTGGCAAGAAATACATAGAGATGGCAGCATCTCTGGACGGGTTGTCTCCTAATGTACCATTGTACAAAGTTACTGAAGGAAATGAACCTCGCTTCTTCACAACATACTTTTCATGGGACCTTGCAAAGGCTAATGTTCAAGGAAACTCATTCCAGAAGAAGGTGTCCGTTCTGTTTGGCCATGCTGTGGAGAGCCAGGACAAATCTGATGGGAATCAAGGAGGGCCAAGGCAAAGAGCTGAAGCCTTAGCTGCTCTATCATCTGCATTCAATTCTTCCCCTGGAAAATCACCCCCTACGGATAAGTCCAATGGGTCGAGCGAAGGAGGACCAAGGCAAAGAGCAGAAGCTTTAGCCGCTTTATCTTCTGCATTTAGTTCATCCTCTGGAACCAAACCTTCTCTTCCAAAGCCTTCTTCAACAAGTCAAGGATCACAAAGAGCTGCAGCAGTTGCTGCACTTTCAAACGTCCTTACAGCTGAAAAGACTAAGTTAACACCTGATGCCTCTCCAGTGCAAAGTCCCCAAGTACAAAGCCCTCCTGCAGAAACTAGTGCTGATACAGATGGTTCTCAGGAAGTTCCTGAAGTAAAGGAAACAGATGCTGTTTCGCCTTCTTCTGTGTCAGAACCAAAGCAAGAGCAGGATGAAAATGGCAGTGAAAGCAGTCAAAGTACTTTCAGTTATGACCAACTGAGGGTTAAATCTGACAATCCAGTAACTGGAATTGATTTTAAGAGAAGAGAGGCCTACCTCTCTGATGAAGACTTTCAGACCATATTTGGGATGACGAAAGATGCTTTCTATCAACTGCCAAAATGGAAGCAAGACATGCAAAAGAAGAAAGCTGATTTGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2

Protein Analysis

966

Amino Acids

106.03

Weight (kDa)

5.38

Isoelectric Point (pI)

42.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Gelsolin PF00626 30 - 111 3.2e-13 Gelsolin repeat
Gelsolin PF00626 149 - 217 1.5e-11 Gelsolin repeat
Gelsolin PF00626 267 - 337 3.1e-12 Gelsolin repeat
DUF7904 PF25480 403 - 491 8.1e-06 Domain of unknown function (DUF7904)
Gelsolin PF00626 409 - 483 1.5e-08 Gelsolin repeat
Gelsolin PF00626 533 - 593 8.2e-07 Gelsolin repeat
Gelsolin PF00626 635 - 713 3.9e-06 Gelsolin repeat
VHP PF02209 931 - 966 5.2e-18 Villin headpiece domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000614)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G40940
fragaria_vesca FvH4_2g32760 FvH4_4g32150 FvH4_4g32590 FvH4_6g20740 FvH4_6g53540 FvH4_7g12190
malus_domestica MD09G1010600.v1.1 MD13G1026900.v1.1 MD13G1028200.v1.1 MD16G1030000.v1.1 MD16G1031100.v1.1
prunus_persica Prupe.1G322000_v2.0.a1 Prupe.3G306500_v2.0.a1
pyrus_communis pycom01g06180 pycom04g10020 pycom111g00880 pycom13g02560 pycom13g02660 pycom16g02710
rosa_chinensis RchiOBHm_Chr2g0175581 RchiOBHm_Chr4g0440841
rosa_laevigata RLG00000006133 RLG00000006134 RLG00000006144 RLG00000008208 RLG00000019874 RLG00000022341 RLG00000032925 RLG00000036410
rosa_multiflora Rmu_co8113152.1_g000001 Rmu_sc0000424.1_g000015 Rmu_sc0000424.1_g000029 Rmu_sc0000424.1_g000030 Rmu_sc0000424.1_g000031 Rmu_sc0000998.1_g000023 Rmu_sc0005054.1_g000012 Rmu_sc0009386.1_g000004 Rmu_sc0013970.1_g000002 Rmu_sc0027146.1_g000001 Rmu_ssc0000175.1_g000015 Rmu_ssc0000386.1_g000026
rosa_roxburghii Rroxscaffold_2G00077200 Rroxscaffold_5G00381670 Rroxscaffold_5G00381840 Rroxscaffold_5G00381850
rosa_rugosa Rorug03G0261000 Rorug04G0120500 Rorug04G0328900 Rorug04G0329900 Rorug04G0330000 Rorug05G0525900
rosa_samantha Rh1AG187300 Rh1BG153500 Rh1CG172800 Rh2BG270100 Rh2BG676400 Rh2CG639600 Rh2DG690400 Rh3BG272200 Rh4AG380600 Rh4AG381600 Rh4AG381700 Rh4BG393200 Rh4BG395000 Rh4BG395100 Rh4CG053800 Rh4CG194900 Rh4CG408000 Rh4CG409900 Rh4CG410000 Rh5BG280900 Rh7AG473300 Rh7BG247300 Rh7CG108400
rosa_wichuraiana Rw2G054570 Rw4G032740 Rw4G032880 Rw4G032890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 35, 451
AccB1I GGYRCC 1 cut(s) 1130
AccB7I CCANNNNNTGG 2 cut(s) 2227, 2762
AccI GTMKAC 1 cut(s) 2010
AccII CGCG 2 cut(s) 475, 1121
AciI CCGC 1 cut(s) 2397
AclWI GGATC 5 cut(s) 20, 33, 1048, 1859, 2479
AcoI YGGCCR 2 cut(s) 1031, 2218
AcsI RAATTY 8 cut(s) 66, 122, 303, 407, 547, 935, 1241, 1739
AcuI CTGAAG 8 cut(s) 885, 1112, 1415, 1766, 2001, 2136, 2301, 2646
AfaI GTAC 5 cut(s) 1280, 2097, 2105, 2571, 2716
AfeI AGCGCT 1 cut(s) 1207
AflIII ACRYGT 3 cut(s) 442, 620, 1001
AgeI ACCGGT 1 cut(s) 1405
AhdI GACNNNNNGTC 1 cut(s) 2083
AhlI ACTAGT 1 cut(s) 2591
AjiI CACGTC 1 cut(s) 284
AjnI CCWGG 5 cut(s) 507, 558, 1750, 2235, 2320
AjuI GAANNNNNNNTTGG 2 cut(s) 2440, 2472
AleI CACNNNNGTG 1 cut(s) 1287
AloI GAACNNNNNNTCC 2 cut(s) 1392, 1424
Alw21I GWGCWC 2 cut(s) 1496, 1793
Alw26I GTCTC 2 cut(s) 992, 2091
AlwI GGATC 5 cut(s) 20, 33, 1048, 1859, 2479
AlwNI CAGNNNCTG 4 cut(s) 665, 2495, 2645, 2762
Aor51HI AGCGCT 1 cut(s) 1207
AoxI GGCC 6 cut(s) 310, 1031, 1486, 2218, 2264, 2787
ApoI RAATTY 8 cut(s) 66, 122, 303, 407, 547, 935, 1241, 1739
AsiGI ACCGGT 1 cut(s) 1405
Asp700I GAANNNNTTC 1 cut(s) 2808
AspA2I CCTAGG 1 cut(s) 1810
AspLEI GCGC 4 cut(s) 475, 1121, 1208, 1456
AspS9I GGNCC 6 cut(s) 685, 1150, 1348, 2158, 2264, 2369
AsuC2I CCSGG 1 cut(s) 979
AsuHPI GGTGA 6 cut(s) 686, 797, 818, 901, 1301, 2322
AsuNHI GCTAGC 2 cut(s) 5, 917
AvaII GGWCC 5 cut(s) 685, 1150, 1348, 2158, 2369
AvrII CCTAGG 1 cut(s) 1810
AxyI CCTNAGG 2 cut(s) 396, 1224
BaeGI GKGCMC 1 cut(s) 1135
BalI TGGCCA 2 cut(s) 1033, 2220
BamHI GGATCC 1 cut(s) 25
BanI GGYRCC 1 cut(s) 1130
BanII GRGCYC 2 cut(s) 1496, 1793
BbsI GAAGAC 2 cut(s) 1408, 2811
Bbv12I GWGCWC 2 cut(s) 1496, 1793
BbvCI CCTCAGC 2 cut(s) 930, 1059
BccI CCATC 6 cut(s) 638, 776, 1174, 2056, 2243, 2600
BceAI ACGGC 1 cut(s) 1589
BciT130I CCWGG 5 cut(s) 509, 560, 1752, 2237, 2322
BciVI GTATCC 1 cut(s) 1963
BcnI CCSGG 1 cut(s) 979
BcoDI GTCTC 2 cut(s) 992, 2091
BcuI ACTAGT 1 cut(s) 2591
BfaI CTAG 7 cut(s) 6, 263, 653, 918, 1811, 2592, 2899
BfmI CTRYAG 3 cut(s) 430, 2484, 2583
BfoI RGCGCY 1 cut(s) 1209
BfuAI ACCTGC 2 cut(s) 35, 451
BfuI GTATCC 1 cut(s) 1963
BglII AGATCT 1 cut(s) 525
BlnI CCTAGG 1 cut(s) 1810
BmcAI AGTACT 1 cut(s) 2716
Bme1390I CCNGG 6 cut(s) 509, 560, 979, 1752, 2237, 2322
Bme18I GGWCC 5 cut(s) 685, 1150, 1348, 2158, 2369
BmeRI GACNNNNNGTC 1 cut(s) 2083
BmgBI CACGTC 1 cut(s) 284
BmgT120I GGNCC 6 cut(s) 685, 1150, 1348, 2158, 2264, 2369
BmiI GGNNCC 9 cut(s) 27, 465, 686, 1132, 1152, 1582, 1669, 2159, 2431
BmrFI CCNGG 6 cut(s) 509, 560, 979, 1752, 2237, 2322
BmsI GCATC 6 cut(s) 386, 1203, 2078, 2533, 2632, 2827
BmtI GCTAGC 2 cut(s) 9, 921
BpiI GAAGAC 2 cut(s) 1408, 2811
BpmI CTGGAG 2 cut(s) 383, 2535
Bpu10I CCTNAGC 4 cut(s) 930, 1059, 1490, 2287
BpuEI CTTGAG 2 cut(s) 1463, 1917
BpuMI CCSGG 1 cut(s) 979
BsaAI YACGTR 1 cut(s) 1278
BsaBI GATNNNNATC 1 cut(s) 2253
BsaJI CCNNGG 5 cut(s) 1426, 1810, 2267, 2320, 2372
BsaWI WCCGGW 1 cut(s) 1405
BsaXI ACNNNNNCTCC 4 cut(s) 1175, 1205, 1511, 1541
Bse118I RCCGGY 1 cut(s) 1405
Bse1I ACTGG 7 cut(s) 92, 855, 1833, 1971, 2552, 2756, 2767
Bse21I CCTNAGG 2 cut(s) 396, 1224
Bse8I GATNNNNATC 1 cut(s) 2253
BseBI CCWGG 5 cut(s) 509, 560, 1752, 2237, 2322
BseDI CCNNGG 5 cut(s) 1426, 1810, 2267, 2320, 2372
BseGI GGATG 8 cut(s) 192, 232, 504, 585, 1306, 2420, 2692, 2832
BseJI GATNNNNATC 1 cut(s) 2253
BseNI ACTGG 7 cut(s) 92, 855, 1833, 1971, 2552, 2756, 2767
BseRI GAGGAG 4 cut(s) 1076, 1358, 1532, 1660
BseSI GKGCMC 1 cut(s) 1135
BsgI GTGCAG 1 cut(s) 2481
Bsh1236I CGCG 2 cut(s) 475, 1121
Bsh1285I CGRYCG 1 cut(s) 886
BshFI GGCC 6 cut(s) 312, 1033, 1488, 2220, 2266, 2789
BshNI GGYRCC 1 cut(s) 1130
BshTI ACCGGT 1 cut(s) 1405
BsiEI CGRYCG 1 cut(s) 886
BsiHKAI GWGCWC 2 cut(s) 1496, 1793
BsiSI CCGG 2 cut(s) 979, 1406
BslFI GGGAC 5 cut(s) 73, 631, 1136, 2171, 2547
BsmAI GTCTC 2 cut(s) 992, 2091
BsmFI GGGAC 5 cut(s) 73, 631, 1136, 2171, 2547
BsmI GAATGC 1 cut(s) 2306
BsnI GGCC 6 cut(s) 312, 1033, 1488, 2220, 2266, 2789
Bsp1286I GDGCHC 3 cut(s) 1135, 1496, 1793
Bsp1407I TGTACA 1 cut(s) 2103
Bsp143I GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
BspACI CCGC 1 cut(s) 2397
BspANI GGCC 6 cut(s) 312, 1033, 1488, 2220, 2266, 2789
BspFNI CGCG 2 cut(s) 475, 1121
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 9 cut(s) 27, 465, 686, 1132, 1152, 1582, 1669, 2159, 2431
BspMAI CTGCAG 3 cut(s) 434, 2488, 2587
BspMI ACCTGC 2 cut(s) 35, 451
BspOI GCTAGC 2 cut(s) 9, 921
BspPI GGATC 5 cut(s) 20, 33, 1048, 1859, 2479
BspT107I GGYRCC 1 cut(s) 1130
BsrFI RCCGGY 1 cut(s) 1405
BsrGI TGTACA 1 cut(s) 2103
BsrI ACTGG 7 cut(s) 92, 855, 1833, 1971, 2552, 2756, 2767
BssAI RCCGGY 1 cut(s) 1405
BssECI CCNNGG 5 cut(s) 1426, 1810, 2267, 2320, 2372
BssMI GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
BssT1I CCWWGG 4 cut(s) 1426, 1810, 2267, 2372
Bst2UI CCWGG 5 cut(s) 509, 560, 1752, 2237, 2322
Bst4CI ACNGT 7 cut(s) 256, 542, 768, 857, 1446, 1621, 1719
Bst6I CTCTTC 4 cut(s) 1063, 1140, 2448, 2776
BstAPI GCANNNNNTGC 4 cut(s) 1733, 2492, 2495, 2872
BstAUI TGTACA 1 cut(s) 2103
BstBAI YACGTR 1 cut(s) 1278
BstC8I GCNNGC 4 cut(s) 7, 235, 919, 1734
BstENI CCTNNNNNAGG 1 cut(s) 2165
BstF5I GGATG 8 cut(s) 192, 232, 504, 585, 1306, 2420, 2692, 2832
BstFNI CGCG 2 cut(s) 475, 1121
BstH2I RGCGCY 1 cut(s) 1209
BstHHI GCGC 4 cut(s) 475, 1121, 1208, 1456
BstKTI GATC 7 cut(s) 28, 528, 798, 1043, 1867, 1942, 2474
BstMAI GTCTC 2 cut(s) 992, 2091
BstMBI GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
BstMCI CGRYCG 1 cut(s) 886
BstNI CCWGG 5 cut(s) 509, 560, 1752, 2237, 2322
BstNSI RCATGY 4 cut(s) 624, 1772, 1979, 2875
BstSCI CCNGG 6 cut(s) 507, 558, 977, 1750, 2235, 2320
BstSFI CTRYAG 3 cut(s) 430, 2484, 2583
BstSLI GKGCMC 1 cut(s) 1135
BstSNI TACGTA 1 cut(s) 1278
BstUI CGCG 2 cut(s) 475, 1121
BstV2I GAAGAC 2 cut(s) 1408, 2811
BstX2I RGATCY 3 cut(s) 25, 525, 1864
BstYI RGATCY 3 cut(s) 25, 525, 1864
Bsu36I CCTNAGG 2 cut(s) 396, 1224
BsuI GTATCC 1 cut(s) 1963
BsuRI GGCC 6 cut(s) 312, 1033, 1488, 2220, 2266, 2789
BtrI CACGTC 1 cut(s) 284
BtsCI GGATG 8 cut(s) 192, 232, 504, 585, 1306, 2420, 2692, 2832
BtsI GCAGTG 1 cut(s) 2704
BtsIMutI CAGTG 5 cut(s) 482, 764, 1391, 2559, 2704
BveI ACCTGC 2 cut(s) 35, 451
Cac8I GCNNGC 4 cut(s) 7, 235, 919, 1734
CaiI CAGNNNCTG 4 cut(s) 665, 2495, 2645, 2762
CciI TCATGA 1 cut(s) 490
CfoI GCGC 4 cut(s) 475, 1121, 1208, 1456
Cfr10I RCCGGY 1 cut(s) 1405
Cfr13I GGNCC 6 cut(s) 685, 1150, 1348, 2158, 2264, 2369
Csp6I GTAC 5 cut(s) 1279, 2096, 2104, 2570, 2715
CspAI ACCGGT 1 cut(s) 1405
CviQI GTAC 5 cut(s) 1279, 2096, 2104, 2570, 2715
DpnI GATC 7 cut(s) 27, 527, 797, 1042, 1866, 1941, 2473
DpnII GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
DraI TTTAAA 2 cut(s) 388, 1764
DriI GACNNNNNGTC 1 cut(s) 2083
EaeI YGGCCR 2 cut(s) 1031, 2218
Eam1104I CTCTTC 4 cut(s) 1063, 1140, 2448, 2776
Eam1105I GACNNNNNGTC 1 cut(s) 2083
EarI CTCTTC 4 cut(s) 1063, 1140, 2448, 2776
Ecl136II GAGCTC 2 cut(s) 1494, 1791
Eco105I TACGTA 1 cut(s) 1278
Eco130I CCWWGG 4 cut(s) 1426, 1810, 2267, 2372
Eco147I AGGCCT 1 cut(s) 2789
Eco24I GRGCYC 2 cut(s) 1496, 1793
Eco32I GATATC 2 cut(s) 1234, 1957
Eco47I GGWCC 5 cut(s) 685, 1150, 1348, 2158, 2369
Eco47III AGCGCT 1 cut(s) 1207
Eco53kI GAGCTC 2 cut(s) 1494, 1791
Eco57I CTGAAG 8 cut(s) 885, 1112, 1415, 1766, 2001, 2136, 2301, 2646
Eco81I CCTNAGG 2 cut(s) 396, 1224
EcoICRI GAGCTC 2 cut(s) 1494, 1791
EcoNI CCTNNNNNAGG 1 cut(s) 2165
EcoO109I RGGNCCY 3 cut(s) 685, 1150, 2158
EcoRI GAATTC 1 cut(s) 547
EcoRII CCWGG 5 cut(s) 507, 558, 1750, 2235, 2320
EcoRV GATATC 2 cut(s) 1234, 1957
EcoT14I CCWWGG 4 cut(s) 1426, 1810, 2267, 2372
EcoT38I GRGCYC 2 cut(s) 1496, 1793
ErhI CCWWGG 4 cut(s) 1426, 1810, 2267, 2372
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 5 cut(s) 73, 631, 1136, 2171, 2547
FblI GTMKAC 1 cut(s) 2010
FokI GGATG 8 cut(s) 179, 239, 491, 572, 1313, 2407, 2699, 2839
FriOI GRGCYC 2 cut(s) 1496, 1793
FspBI CTAG 7 cut(s) 6, 263, 653, 918, 1811, 2592, 2899
GlaI GCGC 4 cut(s) 474, 1120, 1207, 1455
GsuI CTGGAG 2 cut(s) 383, 2535
HaeII RGCGCY 1 cut(s) 1209
HaeIII GGCC 6 cut(s) 312, 1033, 1488, 2220, 2266, 2789
HapII CCGG 2 cut(s) 979, 1406
HhaI GCGC 4 cut(s) 475, 1121, 1208, 1456
Hin6I GCGC 4 cut(s) 473, 1119, 1206, 1454
HinP1I GCGC 4 cut(s) 473, 1119, 1206, 1454
HincII GTYRAC 1 cut(s) 2535
HindII GTYRAC 1 cut(s) 2535
HindIII AAGCTT 2 cut(s) 1730, 2388
HinfI GANTC 8 cut(s) 137, 317, 487, 662, 668, 749, 1025, 2254
HpaI GTTAAC 1 cut(s) 2535
HpaII CCGG 2 cut(s) 979, 1406
HphI GGTGA 6 cut(s) 686, 797, 818, 901, 1301, 2322
Hpy166II GTNNAC 5 cut(s) 753, 809, 1878, 2011, 2535
Hpy8I GTNNAC 5 cut(s) 753, 809, 1878, 2011, 2535
HpyCH4III ACNGT 7 cut(s) 256, 542, 768, 857, 1446, 1621, 1719
HpyCH4IV ACGT 7 cut(s) 283, 442, 1003, 1049, 1277, 1628, 2508
HpySE526I ACGT 7 cut(s) 283, 442, 1003, 1049, 1277, 1628, 2508
HspAI GCGC 4 cut(s) 473, 1119, 1206, 1454
KspAI GTTAAC 1 cut(s) 2535
Kzo9I GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
LmnI GCTCC 3 cut(s) 41, 709, 1673
LweI GCATC 6 cut(s) 386, 1203, 2078, 2533, 2632, 2827
MaeI CTAG 7 cut(s) 6, 263, 653, 918, 1811, 2592, 2899
MaeII ACGT 7 cut(s) 283, 442, 1003, 1049, 1277, 1628, 2508
MaeIII GTNAC 6 cut(s) 480, 889, 1415, 1756, 2110, 2758
MalI GATC 7 cut(s) 27, 527, 797, 1042, 1866, 1941, 2473
MboI GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
MflI RGATCY 3 cut(s) 25, 525, 1864
MhlI GDGCHC 3 cut(s) 1135, 1496, 1793
MlsI TGGCCA 2 cut(s) 1033, 2220
MluNI TGGCCA 2 cut(s) 1033, 2220
MlyI GAGTC 2 cut(s) 662, 758
MmeI TCCRAC 2 cut(s) 1041, 1410
Mox20I TGGCCA 2 cut(s) 1033, 2220
MroXI GAANNNNTTC 1 cut(s) 2808
MscI TGGCCA 2 cut(s) 1033, 2220
MseI TTAA 6 cut(s) 249, 387, 1763, 2534, 2742, 2775
MslI CAYNNNNRTG 3 cut(s) 495, 1287, 2060
Msp20I TGGCCA 2 cut(s) 1033, 2220
MspA1I CMGCKG 2 cut(s) 929, 2519
MspI CCGG 2 cut(s) 979, 1406
MspR9I CCNGG 6 cut(s) 509, 560, 979, 1752, 2237, 2322
Mva1269I GAATGC 1 cut(s) 2306
MvaI CCWGG 5 cut(s) 509, 560, 1752, 2237, 2322
MvnI CGCG 2 cut(s) 475, 1121
NciI CCSGG 1 cut(s) 979
NdeII GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
NheI GCTAGC 2 cut(s) 5, 917
NlaIV GGNNCC 9 cut(s) 27, 465, 686, 1132, 1152, 1582, 1669, 2159, 2431
NmuCI GTSAC 3 cut(s) 480, 889, 1415
NspI RCATGY 4 cut(s) 624, 1772, 1979, 2875
OliI CACNNNNGTG 1 cut(s) 1287
PagI TCATGA 1 cut(s) 490
PceI AGGCCT 1 cut(s) 2789
PciI ACATGT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 1 cut(s) 1625
PctI GAATGC 1 cut(s) 2306
PdmI GAANNNNTTC 1 cut(s) 2808
PfeI GAWTC 6 cut(s) 137, 317, 487, 662, 1025, 2254
PflMI CCANNNNNTGG 2 cut(s) 2227, 2762
PfoI TCCNGGA 2 cut(s) 507, 558
PinAI ACCGGT 1 cut(s) 1405
PleI GAGTC 2 cut(s) 662, 757
PpsI GAGTC 2 cut(s) 662, 757
Ppu21I YACGTR 1 cut(s) 1278
PpuMI RGGWCCY 3 cut(s) 685, 1150, 2158
PscI ACATGT 1 cut(s) 620
Psp124BI GAGCTC 2 cut(s) 1496, 1793
Psp5II RGGWCCY 3 cut(s) 685, 1150, 2158
Psp6I CCWGG 5 cut(s) 507, 558, 1750, 2235, 2320
PspGI CCWGG 5 cut(s) 507, 558, 1750, 2235, 2320
PspN4I GGNNCC 9 cut(s) 27, 465, 686, 1132, 1152, 1582, 1669, 2159, 2431
PspPI GGNCC 6 cut(s) 685, 1150, 1348, 2158, 2264, 2369
PspPPI RGGWCCY 3 cut(s) 685, 1150, 2158
PstI CTGCAG 3 cut(s) 434, 2488, 2587
PstNI CAGNNNCTG 4 cut(s) 665, 2495, 2645, 2762
PsuI RGATCY 3 cut(s) 25, 525, 1864
PvuII CAGCTG 2 cut(s) 929, 2519
RsaI GTAC 5 cut(s) 1280, 2097, 2105, 2571, 2716
RsaNI GTAC 5 cut(s) 1279, 2096, 2104, 2570, 2715
RseI CAYNNNNRTG 3 cut(s) 495, 1287, 2060
SacI GAGCTC 2 cut(s) 1496, 1793
SaqAI TTAA 6 cut(s) 249, 387, 1763, 2534, 2742, 2775
Sau3AI GATC 7 cut(s) 25, 525, 795, 1040, 1864, 1939, 2471
Sau96I GGNCC 6 cut(s) 685, 1150, 1348, 2158, 2264, 2369
ScaI AGTACT 1 cut(s) 2716
SchI GAGTC 2 cut(s) 662, 758
ScrFI CCNGG 6 cut(s) 509, 560, 979, 1752, 2237, 2322
SduI GDGCHC 3 cut(s) 1135, 1496, 1793
SfaNI GCATC 6 cut(s) 386, 1203, 2078, 2533, 2632, 2827
SfcI CTRYAG 3 cut(s) 430, 2484, 2583
SinI GGWCC 5 cut(s) 685, 1150, 1348, 2158, 2369
SmiMI CAYNNNNRTG 3 cut(s) 495, 1287, 2060
SmlI CTYRAG 2 cut(s) 1478, 1932
SmoI CTYRAG 2 cut(s) 1478, 1932
SnaBI TACGTA 1 cut(s) 1278
SpeI ACTAGT 1 cut(s) 2591
SseBI AGGCCT 1 cut(s) 2789
SsiI CCGC 1 cut(s) 2397
SspMI CTAG 7 cut(s) 6, 263, 653, 918, 1811, 2592, 2899
SstI GAGCTC 2 cut(s) 1496, 1793
StuI AGGCCT 1 cut(s) 2789
StyD4I CCNGG 6 cut(s) 507, 558, 977, 1750, 2235, 2320
StyI CCWWGG 4 cut(s) 1426, 1810, 2267, 2372
TaaI ACNGT 7 cut(s) 256, 542, 768, 857, 1446, 1621, 1719
TaiI ACGT 7 cut(s) 286, 445, 1006, 1052, 1280, 1631, 2511
TaqI TCGA 3 cut(s) 418, 1023, 2357
TaqII GACCGA 1 cut(s) 872
TatI WGTACW 3 cut(s) 2103, 2569, 2714
TauI GCSGC 1 cut(s) 2399
TfiI GAWTC 6 cut(s) 137, 317, 487, 662, 1025, 2254
Tru1I TTAA 6 cut(s) 249, 387, 1763, 2534, 2742, 2775
Tru9I TTAA 6 cut(s) 249, 387, 1763, 2534, 2742, 2775
TscAI CASTG 5 cut(s) 489, 771, 1398, 2559, 2704
TseFI GTSAC 3 cut(s) 480, 889, 1415
Tsp45I GTSAC 3 cut(s) 480, 889, 1415
TspGWI ACGGA 3 cut(s) 87, 2197, 2354
TspRI CASTG 5 cut(s) 489, 771, 1398, 2559, 2704
Van91I CCANNNNNTGG 2 cut(s) 2227, 2762
VpaK11BI GGWCC 5 cut(s) 685, 1150, 1348, 2158, 2369
XagI CCTNNNNNAGG 1 cut(s) 2165
XapI RAATTY 8 cut(s) 66, 122, 303, 407, 547, 935, 1241, 1739
XceI RCATGY 4 cut(s) 624, 1772, 1979, 2875
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmaJI CCTAGG 1 cut(s) 1810
XmiI GTMKAC 1 cut(s) 2010
XmnI GAANNNNTTC 1 cut(s) 2808
XspI CTAG 7 cut(s) 6, 263, 653, 918, 1811, 2592, 2899
ZrmI AGTACT 1 cut(s) 2716
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.