FvH4_1g17740

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0000379
Physical Location & Seq
Forward (+)
10318610 .. 10325802
7193 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g17740.t1

Sequence Viewer

Length: 3321 bp
ATGGCCTCATCATCAACTGGTGGCTGGAAGTATGATGTTTTTCTGAGTTTCAGGGGCCCCGACACTCGCAAAGGGATCACGGTCGAGATACACGATCGGCTTAACAGGAGCGGCATTATCACTTTCATGGACAATCCAGATCTTCAAGTAGGGGATGTTATTTCCCCCACTCTCATAAAGGCAATCAAAGAGTCAAGGTTTGCAATTGTAATTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAGCTTAGAGAGATTTGTGTATCCATGGAAGACAACAGGATTTTGCCTCTTTTTTATCAAGTCGATCCTACTGATGTTCGATATCAGAAGAAGAGTTTCGAACAAGCTTTCTCCAAGCATGAAACCTCTGGCCGACATGAACTAGAGATGGTGAAGCAATGGAGAGCTGATTTACAAAAAGTGGCCAATATCTCTGGGTGGAACACAAACGATTTCAAGACTCACAAAGAACTTGTTGACGTCATTGTGGAATCTCTCCGCAGTAAAGTACCACCTGATGCGATTGAATCCACAGGTGATTTTCAAGCATATGAAGCAACTAAACAAGCCATGGATGATGTTATGAAGGCGCTGACAGATGACAAGATCACTGCGGTTGGTGTTTTCGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAAAAATGGTACTTTTAATCATTGGATTATGGCTGTCGTGTCCCAAAACCGTGACTTGAAAAGAATTCAAGACACATTTGCAGACCTGTTGGGATTTGAATTAAAAGAGAAGACAGAAGATGGAAGAGCCGCTAGGTTGTATAAGGAGATAATGAGAAAAGAAAAACTCCTGATAATAGTGGACGATGTTTGGGAGAGAACAGCATTGTCAAGCATAGGGATTCCCAGCTATAAGGAACTTCAAAAGTGCATGTCCAAAGTGCTACTCACCACAAGGAGATTCAATGTATGTCATGCCATGAAATGCGAAGAAAAGATCGCCCTTAAGGTTCTCTCAGAAAAAGATTCTTGGAACTTGTTTCTGAGAAATGCAGGAACAATTTCTTTTGAGTCCACCGCATTTGAGAAAATAGCAAGGATGGTAGCTGGAGAATGTAAGGGTCTACCTATTGCTTTGATAGCAGTTGCAAGGGCACTCGGAGATAAGGATCTGGCGGAATGGAAAAAAGCAGCTGTACGACTAGAGAATTCGCAATATGTCAATCCCAACCATGAGGAAGATGAGGAAAATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAAAATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATGACATCCAACTAGAAAACTTATTCAAGTATGCATTTGGGATAGGATTGTTTCAAGTTGCGGAAACAACAATTGAAGAAGCCAGAGAAGCAGCAGATTCAGTGATCAAGTACCTGAAAGGTTCTAGCTTGCTCTTGGATAGCGATAACAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCCTGAATATTGCACAATCTGAAGATGGGCATTGGTTTTTGGTGAAAGCTGGTTGTGGTTTAAAGGATTGGCCGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTGATGAGGAACAAAATTCGCAAGCTACCCGAAAAAGAGTTGGTATGTCCAAATCTCCAGATTCTATTGCTAAACGGGAATGCTGATTTAAGTGAGATCCCAAGAAAGTTGATCCAGAATCTGAGAGAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCTGTATTACCCCAACCATTTAGCCTCCTCACCAACCTCCAAGCTTTGTATTTAGACTTTTGTAAGAAATTGATTGAAATTTCCATAGTGGGATCACTGAAAAAGCTTGAAATTCTTAGCATGAGAGAATGTTCTTTCAAGGAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAATCGTGATTGGGAACGGTTCACAATTCCATCTGAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACAGTGGAACTAAGGGACGAGGAGAAGAAACCAATATTGGGTTTGGTGAGTTAGCTGGTTTATCAAACTTGAAAATATTGCAGGTTGGCATATCTGATGAAAAATACATCCCTAAAAATGTTGAGGCCGCACCAGATTGGGATTACTTTTGTATAAGTATCATAAGCAGATGCACCAACTCTGAATACATGTCCGCTGAATACATGCCTGCATACAGAACAGGAGATCATAATTCAAGATCCTTGTTTCTTAGTGGAGTAACCATAAGTACCTTACCTGATTGGTTTATCAACGTGACAGAGAAAACAGAGAAGCTAGAGTATGATCGTTGCAATGGGATGCGTGACATTCTTATGGAATATGACCATGGGAGGTTACATGAACTCAAGCATCTCATAGTTGGTGGGTCTGTTAGTGATTCCGATGTGTGCTTGAAAGAGTTGATGAACACAACAAGGCGAGTTCAAACAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGCTACAACTGATCCACCTTGAGGAGTTGTGTGTTGGTGAGCTACCACCTGAGTCTCTGTCAAATCTGAAGGTATTGCATCTGAATGATTGTTTTATCTTGAAGAGTATATCAAAATTTGTACAGAGACTACCAAATCTGGAGAACCTATATTTAAATAGGATGAAGGAATTGGAATATGTGTTTGGGTGTGAGGAGTTTGAGCCAGAACAATCAAAACTGAGGGAGATGCATTTGTTGTATCTAAAAGCACTAAGAAGCGTATGTAATGGTCCCGCTCCCCGTGCAATGTTCCAGAGTCTTAAAACCTTGACTGTTTACTATTGCAACTTGATGGAAAGTCTGTTCAGATATGATGTAGCTCAGTGTCTCTTTCAATTGGAAGACCTTTATGTAGACGGTTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAACGAGAAGACGGTTCTACCAAAATTGAAGAACTTGGTTTTGAAGAATCTTCCTATGTTGTATGGTCCAAGTGCTACTGTTGATATTAAGTGTCCTTCATTGGAACGCTTGGTGGTGGTGGATTGCCCCAAGCTTTCATTTTCAGCCTCTTCTGATCCCTTCGGCTCCTTCAAAAGCACAAACCAGTTTTCATTTTCAACCTCGGCTTCTGACTATTTTGGCAGCTTGAACCCAGTCCAACTCAATGATCTGGAACTGTACCAGCATCTACGTGACAGACTGAGAATCTAG

Protein Analysis

1107

Amino Acids

125.91

Weight (kDa)

5.86

Isoelectric Point (pI)

39.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 173 1.9e-43 TIR domain
TIR_2 PF13676 13 - 107 6.2e-14 TIR domain
NB-ARC PF00931 192 - 356 5e-29 NB-ARC domain
LRR_8 PF13855 569 - 627 5.1e-09 Leucine rich repeat
LRR_14 PF23598 574 - 701 4e-16 Leucine-rich repeat region
LRR_14 PF23598 636 - 735 6.2e-09 Leucine-rich repeat region
LRR_RPS2 PF23247 860 - 999 1.3e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 958 - 1056 6.5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 495
Acc36I ACCTGC 1 cut(s) 2161
AccBSI CCGCTC 2 cut(s) 111, 2870
AccI GTMKAC 2 cut(s) 1138, 2988
AccII CGCG 1 cut(s) 1626
AcoI YGGCCR 3 cut(s) 382, 435, 1616
AcsI RAATTY 7 cut(s) 759, 1222, 1671, 1896, 1929, 2575, 2708
AcuI CTGAAG 3 cut(s) 1587, 2064, 2681
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 9 cut(s) 522, 706, 1212, 1469, 2081, 2090, 2359, 2715, 3290
AfiI CCNNNNNNNGG 5 cut(s) 1625, 1995, 2127, 2614, 3004
AflII CTTAAG 1 cut(s) 1019
AflIII ACRYGT 2 cut(s) 676, 2277
AhdI GACNNNNNGTC 1 cut(s) 1318
AhlI ACTAGT 1 cut(s) 1811
AjuI GAANNNNNNNTTGG 4 cut(s) 2109, 2141, 2760, 2792
AloI GAACNNNNNNTCC 2 cut(s) 2921, 2953
Alw26I GTCTC 4 cut(s) 667, 2652, 2713, 2966
AlwNI CAGNNNCTG 1 cut(s) 1777
AoxI GGCC 6 cut(s) 3, 55, 382, 435, 1616, 2214
ApaI GGGCCC 1 cut(s) 59
ApeKI GCWGC 5 cut(s) 683, 1205, 1448, 1641, 3252
ApoI RAATTY 7 cut(s) 759, 1222, 1671, 1896, 1929, 2575, 2708
Asp700I GAANNNNTTC 2 cut(s) 347, 1075
AspLEI GCGC 1 cut(s) 604
AspS9I GGNCC 5 cut(s) 55, 56, 2561, 2864, 3095
AsuC2I CCSGG 1 cut(s) 1538
AsuHPI GGTGA 8 cut(s) 415, 560, 682, 955, 1600, 1840, 2147, 2642
AsuII TTCGAA 1 cut(s) 351
AvaII GGWCC 3 cut(s) 2561, 2864, 3095
BaeGI GKGCMC 2 cut(s) 59, 1171
BalI TGGCCA 1 cut(s) 437
BanII GRGCYC 1 cut(s) 59
BbsI GAAGAC 6 cut(s) 288, 812, 1317, 1359, 2982, 3044
BbvI GCAGC 5 cut(s) 670, 1217, 1460, 1628, 3264
BccI CCATC 6 cut(s) 394, 809, 1108, 1565, 2047, 2920
BciVI GTATCC 2 cut(s) 283, 1534
BclI TGATCA 1 cut(s) 1461
BcnI CCSGG 1 cut(s) 1538
BcoDI GTCTC 4 cut(s) 667, 2652, 2713, 2966
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 8 cut(s) 395, 828, 1217, 1370, 1482, 1812, 2405, 3319
BfoI RGCGCY 1 cut(s) 605
BfrI CTTAAG 1 cut(s) 1019
BfuAI ACCTGC 1 cut(s) 2161
BfuI GTATCC 2 cut(s) 283, 1534
BglII AGATCT 1 cut(s) 139
Bme1390I CCNGG 1 cut(s) 1538
Bme18I GGWCC 3 cut(s) 2561, 2864, 3095
BmeRI GACNNNNNGTC 1 cut(s) 1318
BmgT120I GGNCC 5 cut(s) 55, 56, 2561, 2864, 3095
BmiI GGNNCC 5 cut(s) 56, 57, 58, 2866, 3196
BmrFI CCNGG 1 cut(s) 1538
BmrI ACTGGG 1 cut(s) 3257
BmsI GCATC 9 cut(s) 520, 1512, 2249, 2418, 2488, 2599, 2680, 2811, 3304
BmuI ACTGGG 1 cut(s) 3257
BpiI GAAGAC 6 cut(s) 288, 812, 1317, 1359, 2982, 3044
BpmI CTGGAG 3 cut(s) 1143, 1697, 2753
Bpu14I TTCGAA 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 2459, 2633
BpuMI CCSGG 1 cut(s) 1538
BsaAI YACGTR 1 cut(s) 3302
BsaBI GATNNNNATC 2 cut(s) 1182, 2010
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 4 cut(s) 276, 582, 2455, 3231
Bsc4I CCNNNNNNNGG 5 cut(s) 1625, 1995, 2127, 2614, 3004
Bse1I ACTGG 4 cut(s) 22, 2564, 3214, 3263
Bse3DI GCAATG 3 cut(s) 416, 2428, 2886
Bse8I GATNNNNATC 2 cut(s) 1182, 2010
BseDI CCNNGG 4 cut(s) 276, 582, 2455, 3231
BseJI GATNNNNATC 2 cut(s) 1182, 2010
BseLI CCNNNNNNNGG 5 cut(s) 1625, 1995, 2127, 2614, 3004
BseMI GCAATG 3 cut(s) 416, 2428, 2886
BseMII CTCAG 8 cut(s) 35, 1044, 1049, 1769, 2634, 2804, 2969, 3302
BseNI ACTGG 4 cut(s) 22, 2564, 3214, 3263
BseRI GAGGAG 5 cut(s) 266, 1835, 2124, 2630, 2801
BseSI GKGCMC 2 cut(s) 59, 1171
BseXI GCAGC 5 cut(s) 670, 1217, 1460, 1628, 3264
BseYI CCCAGC 1 cut(s) 920
BsgI GTGCAG 2 cut(s) 669, 1627
Bsh1236I CGCG 1 cut(s) 1626
Bsh1285I CGRYCG 2 cut(s) 84, 97
BshFI GGCC 6 cut(s) 5, 57, 384, 437, 1618, 2216
BsiEI CGRYCG 2 cut(s) 84, 97
BsiSI CCGG 1 cut(s) 1537
BslFI GGGAC 3 cut(s) 721, 2118, 2850
BslI CCNNNNNNNGG 5 cut(s) 1625, 1995, 2127, 2614, 3004
BsmAI GTCTC 4 cut(s) 667, 2652, 2713, 2966
BsmFI GGGAC 3 cut(s) 721, 2118, 2850
BsmI GAATGC 2 cut(s) 1268, 1741
BsnI GGCC 6 cut(s) 5, 57, 384, 437, 1618, 2216
Bsp119I TTCGAA 1 cut(s) 351
Bsp120I GGGCCC 1 cut(s) 55
Bsp1286I GDGCHC 2 cut(s) 59, 1171
Bsp1407I TGTACA 3 cut(s) 2079, 2088, 2713
Bsp19I CCATGG 3 cut(s) 276, 582, 2455
BspANI GGCC 6 cut(s) 5, 57, 384, 437, 1618, 2216
BspCNI CTCAG 8 cut(s) 36, 1043, 1050, 1770, 2635, 2805, 2968, 3303
BspFNI CGCG 1 cut(s) 1626
BspLI GGNNCC 5 cut(s) 56, 57, 58, 2866, 3196
BspMI ACCTGC 1 cut(s) 2161
BspQI GCTCTTC 1 cut(s) 814
BspT104I TTCGAA 1 cut(s) 351
BspTI CTTAAG 1 cut(s) 1019
BsrBI CCGCTC 2 cut(s) 111, 2870
BsrDI GCAATG 3 cut(s) 416, 2428, 2886
BsrGI TGTACA 3 cut(s) 2079, 2088, 2713
BsrI ACTGG 4 cut(s) 22, 2564, 3214, 3263
BssECI CCNNGG 4 cut(s) 276, 582, 2455, 3231
BssNI GRCGYC 1 cut(s) 492
BssT1I CCWWGG 3 cut(s) 276, 582, 2455
Bst4CI ACNGT 9 cut(s) 82, 746, 2028, 2093, 2908, 2993, 3043, 3109, 3288
Bst6I CTCTTC 5 cut(s) 338, 814, 2577, 2690, 3184
BstACI GRCGYC 1 cut(s) 492
BstAFI CTTAAG 1 cut(s) 1019
BstAUI TGTACA 3 cut(s) 2079, 2088, 2713
BstBAI YACGTR 1 cut(s) 3302
BstBI TTCGAA 1 cut(s) 351
BstC8I GCNNGC 4 cut(s) 694, 1487, 1679, 2298
BstDSI CCRYGG 3 cut(s) 276, 582, 2455
BstFNI CGCG 1 cut(s) 1626
BstH2I RGCGCY 1 cut(s) 605
BstHHI GCGC 1 cut(s) 604
BstMAI GTCTC 4 cut(s) 667, 2652, 2713, 2966
BstMCI CGRYCG 2 cut(s) 84, 97
BstMWI GCNNNNNNNGC 6 cut(s) 566, 648, 689, 1154, 1445, 2876
BstNSI RCATGY 4 cut(s) 680, 949, 2281, 2296
BstSCI CCNGG 1 cut(s) 1536
BstSLI GKGCMC 2 cut(s) 59, 1171
BstUI CGCG 1 cut(s) 1626
BstV1I GCAGC 5 cut(s) 670, 1217, 1460, 1628, 3264
BstV2I GAAGAC 6 cut(s) 288, 812, 1317, 1359, 2982, 3044
BstX2I RGATCY 5 cut(s) 139, 1183, 1752, 1798, 2327
BstYI RGATCY 5 cut(s) 139, 1183, 1752, 1798, 2327
BsuI GTATCC 2 cut(s) 283, 1534
BsuRI GGCC 6 cut(s) 5, 57, 384, 437, 1618, 2216
BtgI CCRYGG 3 cut(s) 276, 582, 2455
BtsI GCAGTG 3 cut(s) 621, 1644, 3030
BtsIMutI CAGTG 9 cut(s) 621, 1464, 1644, 1655, 1913, 2098, 2571, 2963, 3030
BveI ACCTGC 1 cut(s) 2161
Cac8I GCNNGC 4 cut(s) 694, 1487, 1679, 2298
CaiI CAGNNNCTG 1 cut(s) 1777
CfoI GCGC 1 cut(s) 604
Cfr13I GGNCC 5 cut(s) 55, 56, 2561, 2864, 3095
Csp6I GTAC 9 cut(s) 521, 705, 1211, 1468, 2080, 2089, 2358, 2714, 3289
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviQI GTAC 9 cut(s) 521, 705, 1211, 1468, 2080, 2089, 2358, 2714, 3289
DraI TTTAAA 2 cut(s) 1608, 2748
DriI GACNNNNNGTC 1 cut(s) 1318
EaeI YGGCCR 3 cut(s) 382, 435, 1616
Eam1104I CTCTTC 5 cut(s) 338, 814, 2577, 2690, 3184
Eam1105I GACNNNNNGTC 1 cut(s) 1318
EarI CTCTTC 5 cut(s) 338, 814, 2577, 2690, 3184
EciI GGCGGA 1 cut(s) 1205
Eco130I CCWWGG 3 cut(s) 276, 582, 2455
Eco24I GRGCYC 1 cut(s) 59
Eco32I GATATC 2 cut(s) 335, 2052
Eco47I GGWCC 3 cut(s) 2561, 2864, 3095
Eco57I CTGAAG 3 cut(s) 1587, 2064, 2681
EcoO109I RGGNCCY 2 cut(s) 55, 56
EcoRI GAATTC 2 cut(s) 759, 1222
EcoRV GATATC 2 cut(s) 335, 2052
EcoT14I CCWWGG 3 cut(s) 276, 582, 2455
EcoT22I ATGCAT 4 cut(s) 1270, 1393, 1527, 2826
EcoT38I GRGCYC 1 cut(s) 59
ErhI CCWWGG 3 cut(s) 276, 582, 2455
FalI AAGNNNNNCTT 2 cut(s) 1310, 1342
FaqI GGGAC 3 cut(s) 721, 2118, 2850
FauI CCCGC 1 cut(s) 2875
FauNDI CATATG 1 cut(s) 562
FbaI TGATCA 1 cut(s) 1461
FblI GTMKAC 2 cut(s) 1138, 2988
FriOI GRGCYC 1 cut(s) 59
FspBI CTAG 8 cut(s) 395, 828, 1217, 1370, 1482, 1812, 2405, 3319
GlaI GCGC 1 cut(s) 603
GsaI CCCAGC 1 cut(s) 924
GsuI CTGGAG 3 cut(s) 1143, 1697, 2753
HaeII RGCGCY 1 cut(s) 605
HaeIII GGCC 6 cut(s) 5, 57, 384, 437, 1618, 2216
HapII CCGG 1 cut(s) 1537
HhaI GCGC 1 cut(s) 604
Hin1I GRCGYC 1 cut(s) 492
Hin6I GCGC 1 cut(s) 602
HinP1I GCGC 1 cut(s) 602
HincII GTYRAC 1 cut(s) 490
HindII GTYRAC 1 cut(s) 490
HindIII AAGCTT 4 cut(s) 357, 1860, 1922, 3161
HpaII CCGG 1 cut(s) 1537
HphI GGTGA 8 cut(s) 415, 560, 682, 955, 1600, 1840, 2147, 2642
Hpy166II GTNNAC 9 cut(s) 490, 877, 1089, 1139, 2031, 2089, 2911, 2989, 3028
Hpy8I GTNNAC 9 cut(s) 490, 877, 1089, 1139, 2031, 2089, 2911, 2989, 3028
HpyAV CCTTC 7 cut(s) 592, 1631, 2656, 2752, 3135, 3199, 3208
HpyCH4III ACNGT 9 cut(s) 82, 746, 2028, 2093, 2908, 2993, 3043, 3109, 3288
HpyCH4IV ACGT 3 cut(s) 492, 2382, 3301
HpyF10VI GCNNNNNNNGC 6 cut(s) 566, 648, 689, 1154, 1445, 2876
HpySE526I ACGT 3 cut(s) 492, 2382, 3301
Hsp92I GRCGYC 1 cut(s) 492
HspAI GCGC 1 cut(s) 602
Ksp22I TGATCA 1 cut(s) 1461
LguI GCTCTTC 1 cut(s) 814
LmnI GCTCC 4 cut(s) 108, 253, 2875, 3200
Lsp1109I GCAGC 5 cut(s) 670, 1217, 1460, 1628, 3264
LweI GCATC 9 cut(s) 520, 1512, 2249, 2418, 2488, 2599, 2680, 2811, 3304
MaeI CTAG 8 cut(s) 395, 828, 1217, 1370, 1482, 1812, 2405, 3319
MaeII ACGT 3 cut(s) 492, 2382, 3301
MaeIII GTNAC 6 cut(s) 746, 2347, 2383, 2432, 2463, 3302
MbiI CCGCTC 2 cut(s) 111, 2870
MfeI CAATTG 3 cut(s) 204, 1428, 2969
MflI RGATCY 5 cut(s) 139, 1183, 1752, 1798, 2327
MhlI GDGCHC 2 cut(s) 59, 1171
MlsI TGGCCA 1 cut(s) 437
MluNI TGGCCA 1 cut(s) 437
MlyI GAGTC 5 cut(s) 200, 466, 1094, 2654, 2899
MmeI TCCRAC 4 cut(s) 634, 1390, 2985, 3292
Mox20I TGGCCA 1 cut(s) 437
Mph1103I ATGCAT 4 cut(s) 1270, 1393, 1527, 2826
MroXI GAANNNNTTC 2 cut(s) 347, 1075
MscI TGGCCA 1 cut(s) 437
MslI CAYNNNNRTG 5 cut(s) 125, 1273, 2441, 2676, 3300
Msp20I TGGCCA 1 cut(s) 437
MspA1I CMGCKG 2 cut(s) 1208, 2285
MspCI CTTAAG 1 cut(s) 1019
MspI CCGG 1 cut(s) 1537
MspR9I CCNGG 1 cut(s) 1538
MunI CAATTG 3 cut(s) 204, 1428, 2969
Mva1269I GAATGC 2 cut(s) 1268, 1741
MvnI CGCG 1 cut(s) 1626
MwoI GCNNNNNNNGC 6 cut(s) 566, 648, 689, 1154, 1445, 2876
NciI CCSGG 1 cut(s) 1538
NcoI CCATGG 3 cut(s) 276, 582, 2455
NdeI CATATG 1 cut(s) 562
NlaIV GGNNCC 5 cut(s) 56, 57, 58, 2866, 3196
NmeAIII GCCGAG 1 cut(s) 3212
NmuCI GTSAC 4 cut(s) 746, 2383, 2432, 3302
NsiI ATGCAT 4 cut(s) 1270, 1393, 1527, 2826
NspI RCATGY 4 cut(s) 680, 949, 2281, 2296
NspV TTCGAA 1 cut(s) 351
PciI ACATGT 2 cut(s) 676, 2277
PciSI GCTCTTC 1 cut(s) 814
PcsI WCGNNNNNNNCGW 1 cut(s) 90
PctI GAATGC 2 cut(s) 1268, 1741
PdmI GAANNNNTTC 2 cut(s) 347, 1075
PfoI TCCNGGA 1 cut(s) 1536
Ple19I CGATCG 1 cut(s) 97
PleI GAGTC 5 cut(s) 199, 466, 1093, 2653, 2898
PpsI GAGTC 5 cut(s) 199, 466, 1093, 2653, 2898
Ppu21I YACGTR 1 cut(s) 3302
PscI ACATGT 2 cut(s) 676, 2277
PspFI CCCAGC 1 cut(s) 920
PspN4I GGNNCC 5 cut(s) 56, 57, 58, 2866, 3196
PspOMI GGGCCC 1 cut(s) 55
PspPI GGNCC 5 cut(s) 55, 56, 2561, 2864, 3095
PstNI CAGNNNCTG 1 cut(s) 1777
PsuI RGATCY 5 cut(s) 139, 1183, 1752, 1798, 2327
PvuI CGATCG 1 cut(s) 97
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 9 cut(s) 522, 706, 1212, 1469, 2081, 2090, 2359, 2715, 3290
RsaNI GTAC 9 cut(s) 521, 705, 1211, 1468, 2080, 2089, 2358, 2714, 3289
RseI CAYNNNNRTG 5 cut(s) 125, 1273, 2441, 2676, 3300
SapI GCTCTTC 1 cut(s) 814
Sau96I GGNCC 5 cut(s) 55, 56, 2561, 2864, 3095
SchI GAGTC 5 cut(s) 200, 466, 1094, 2654, 2899
ScrFI CCNGG 1 cut(s) 1538
SduI GDGCHC 2 cut(s) 59, 1171
SfaNI GCATC 9 cut(s) 520, 1512, 2249, 2418, 2488, 2599, 2680, 2811, 3304
SfuI TTCGAA 1 cut(s) 351
SinI GGWCC 3 cut(s) 2561, 2864, 3095
SmiI ATTTAAAT 1 cut(s) 2748
SmiMI CAYNNNNRTG 5 cut(s) 125, 1273, 2441, 2676, 3300
SmlI CTYRAG 3 cut(s) 1019, 2474, 2612
SmoI CTYRAG 3 cut(s) 1019, 2474, 2612
SpeI ACTAGT 1 cut(s) 1811
SspI AATATT 3 cut(s) 1555, 2125, 2166
SspMI CTAG 8 cut(s) 395, 828, 1217, 1370, 1482, 1812, 2405, 3319
StyD4I CCNGG 1 cut(s) 1536
StyI CCWWGG 3 cut(s) 276, 582, 2455
SwaI ATTTAAAT 1 cut(s) 2748
TaaI ACNGT 9 cut(s) 82, 746, 2028, 2093, 2908, 2993, 3043, 3109, 3288
TaiI ACGT 3 cut(s) 495, 2385, 3304
TaqI TCGA 5 cut(s) 84, 315, 331, 351, 1967
TatI WGTACW 3 cut(s) 2079, 2088, 2713
TauI GCSGC 5 cut(s) 114, 651, 827, 1621, 2219
TscAI CASTG 9 cut(s) 628, 1464, 1651, 1662, 1920, 2098, 2571, 2963, 3030
TseFI GTSAC 4 cut(s) 746, 2383, 2432, 3302
TseI GCWGC 5 cut(s) 683, 1205, 1448, 1641, 3252
Tsp45I GTSAC 4 cut(s) 746, 2383, 2432, 3302
TspRI CASTG 9 cut(s) 628, 1464, 1651, 1662, 1920, 2098, 2571, 2963, 3030
Vha464I CTTAAG 1 cut(s) 1019
VpaK11BI GGWCC 3 cut(s) 2561, 2864, 3095
XapI RAATTY 7 cut(s) 759, 1222, 1671, 1896, 1929, 2575, 2708
XceI RCATGY 4 cut(s) 680, 949, 2281, 2296
XmiI GTMKAC 2 cut(s) 1138, 2988
XmnI GAANNNNTTC 2 cut(s) 347, 1075
XspI CTAG 8 cut(s) 395, 828, 1217, 1370, 1482, 1812, 2405, 3319
ZraI GACGTC 1 cut(s) 493
Zsp2I ATGCAT 4 cut(s) 1270, 1393, 1527, 2826
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.