RchiOBHm_Chr5g0079341

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
85021928 .. 85023373
1446 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35376

Sequence Viewer

Length: 1446 bp
ATGGGAGAGTTCGAAGAGTTTCAAGCAACGAGACAAGCCATGAATGAAGTTATGAAGGCGCTAAAAGATGATGAAGTCGATGCCGTTGGGGTGTTCGGCATGGGAGGTGTCGGCAAGACAACCATGGTAAACCATGTGGTAAACCATGTCGGTGCACAAGCCCGCAAAAATTGGATTTTTCATCATGTGATTATGGCTGTCGTTTCACAAAATCCTAATTTCGAAAAACTTCAAGACGCGTTGGCAGAGCAATTAGGCTTCAAACTCTGGGAGCAAACAGAAATTGGAAGAATTGCTAGTTTGAATAAGGAGATAATGAGAAGAGAAAAACTCCTTATAATCTTGGATGATGTTTGGGGGAGAATAGAGTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCACAAGGATAAAGAATGTCTGTCATGTCAAGAGATGCCAAAGAAAGATCACCCTCGGTGTCTTATCAGAACAAGATTCTTGGACCTTGTTTCTGAGAAATGCAGGAATAATGTCTTTTGAGTCCACCACATTTGAGAATGTGGCGAGAAGGGTAGCTGGAGAATGTAAGGGTCTACCGATTGCATTGATAGCAGTTGCAAGAGCACTCGGAGATAAAGACTTAGCGGAATGGGAAAAAGCAGCTCAACGACTAGAGAAGTCTCAATATGCCAATCCTAACCATGAGGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAACATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGTCTATACCCAGAAGACCATGACATCCGACTAGAAAACTTATTCAAGTATGCAATCGGGACAGGATTGTTTCGACGAGATGCCGAAACAATCGAAGAAGCTAGAGGAATAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGCGAGAAAAAGGGATATGTAAAGATGCATGATGTCATCCGGGATGCAGCCCTGAATATTGCAAAATCTGAAGACGGGCATGGGTTTTTGGTAAAAGCTGGCTGTGGTATGGAGGATTGGCTGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAAGATTCGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAAACGGAACGCTGGTTTAAATGAGATCCCAGAAAAATTTATCCAAAGTCTTAAGGAATTAAGGGTATTGGATCTTAGCAACACTAGTATTTCCTTACTACCCCAATCATTCAGTCTCCTTATCAACCTCCAAGCTTTGTATTTAGATTTTTGTGAGAAATTGATTGACATTTCCATAGTGGGAAAACTTAAGAAGCTTGAAATTCTTAGTATAAGAGAATATCCTCTCAAGGAATCGTCCAGAGAAATAGGACATTAG

Protein Analysis

481

Amino Acids

54.64

Weight (kDa)

7.18

Isoelectric Point (pI)

44.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 12 - 179 5.5e-29 NB-ARC domain
LRR_8 PF13855 389 - 447 3.8e-09 Leucine rich repeat
LRR_14 PF23598 394 - 470 3.4e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 338
AccI GTMKAC 1 cut(s) 607
AccII CGCG 2 cut(s) 239, 1086
AciI CCGC 3 cut(s) 163, 659, 1086
AclWI GGATC 2 cut(s) 1207, 1266
AcsI RAATTY 2 cut(s) 1223, 1389
AcuI CTGAAG 1 cut(s) 1047
AfaI GTAC 1 cut(s) 929
AflII CTTAAG 2 cut(s) 1238, 1376
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 9 cut(s) 23, 233, 262, 304, 407, 733, 763, 844, 1388
AhdI GACNNNNNGTC 1 cut(s) 778
AhlI ACTAGT 1 cut(s) 1271
Alw21I GWGCWC 2 cut(s) 157, 640
Alw26I GTCTC 3 cut(s) 25, 699, 1307
Alw44I GTGCAC 1 cut(s) 153
AlwI GGATC 2 cut(s) 1207, 1266
ApaLI GTGCAC 1 cut(s) 153
ApeKI GCWGC 4 cut(s) 674, 1004, 1078, 1101
ApoI RAATTY 2 cut(s) 1223, 1389
Asp700I GAANNNNTTC 2 cut(s) 18, 228
AspLEI GCGC 1 cut(s) 61
AspS9I GGNCC 1 cut(s) 516
AsuC2I CCSGG 1 cut(s) 998
AsuHPI GGTGA 2 cut(s) 424, 475
AsuII TTCGAA 2 cut(s) 12, 222
AvaII GGWCC 1 cut(s) 516
BaeGI GKGCMC 1 cut(s) 157
BbsI GAAGAC 3 cut(s) 777, 819, 1035
Bbv12I GWGCWC 2 cut(s) 157, 640
BbvI GCAGC 4 cut(s) 686, 1016, 1065, 1088
BceAI ACGGC 1 cut(s) 68
BcnI CCSGG 1 cut(s) 998
BcoDI GTCTC 3 cut(s) 25, 699, 1307
BcuI ACTAGT 1 cut(s) 1271
BfaI CTAG 6 cut(s) 297, 686, 830, 900, 942, 1272
BfoI RGCGCY 1 cut(s) 62
BfrI CTTAAG 2 cut(s) 1238, 1376
BisI GCNGC 4 cut(s) 675, 1005, 1079, 1102
BlsI GCNGC 4 cut(s) 676, 1006, 1080, 1103
Bme1390I CCNGG 1 cut(s) 998
Bme18I GGWCC 1 cut(s) 516
BmeRI GACNNNNNGTC 1 cut(s) 778
BmgT120I GGNCC 1 cut(s) 516
BmrFI CCNGG 1 cut(s) 998
BmsI GCATC 6 cut(s) 70, 458, 715, 868, 972, 991
BpiI GAAGAC 3 cut(s) 777, 819, 1035
BplI GAGNNNNNCTC 2 cut(s) 315, 347
BpmI CTGGAG 2 cut(s) 612, 1157
Bpu14I TTCGAA 2 cut(s) 12, 222
BpuEI CTTGAG 1 cut(s) 1400
BpuMI CCSGG 1 cut(s) 998
BsaJI CCNNGG 2 cut(s) 123, 487
BseDI CCNNGG 2 cut(s) 123, 487
BseGI GGATG 4 cut(s) 352, 822, 993, 1006
BseMII CTCAG 1 cut(s) 518
BseSI GKGCMC 1 cut(s) 157
BseXI GCAGC 4 cut(s) 686, 1016, 1065, 1088
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 1 cut(s) 1087
Bsh1236I CGCG 2 cut(s) 239, 1086
BsiHKAI GWGCWC 2 cut(s) 157, 640
BsiSI CCGG 1 cut(s) 997
BslFI GGGAC 1 cut(s) 871
BsmAI GTCTC 3 cut(s) 25, 699, 1307
BsmFI GGGAC 1 cut(s) 871
BsmI GAATGC 1 cut(s) 728
Bsp119I TTCGAA 2 cut(s) 12, 222
Bsp1286I GDGCHC 2 cut(s) 157, 640
Bsp143I GATC 3 cut(s) 480, 1212, 1258
Bsp19I CCATGG 1 cut(s) 123
BspACI CCGC 3 cut(s) 163, 659, 1086
BspCNI CTCAG 1 cut(s) 519
BspFNI CGCG 2 cut(s) 239, 1086
BspPI GGATC 2 cut(s) 1207, 1266
BspT104I TTCGAA 2 cut(s) 12, 222
BspTI CTTAAG 2 cut(s) 1238, 1376
BssECI CCNNGG 2 cut(s) 123, 487
BssMI GATC 3 cut(s) 480, 1212, 1258
BssT1I CCWWGG 1 cut(s) 123
Bst6I CTCTTC 3 cut(s) 9, 316, 1146
BstAFI CTTAAG 2 cut(s) 1238, 1376
BstBI TTCGAA 2 cut(s) 12, 222
BstC8I GCNNGC 4 cut(s) 163, 947, 1057, 1139
BstDEI CTNAG 4 cut(s) 527, 655, 1262, 1394
BstDSI CCRYGG 1 cut(s) 123
BstF5I GGATG 4 cut(s) 352, 822, 993, 1006
BstFNI CGCG 2 cut(s) 239, 1086
BstH2I RGCGCY 1 cut(s) 62
BstHHI GCGC 1 cut(s) 61
BstKTI GATC 3 cut(s) 483, 1215, 1261
BstMAI GTCTC 3 cut(s) 25, 699, 1307
BstMBI GATC 3 cut(s) 480, 1212, 1258
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstSCI CCNGG 1 cut(s) 996
BstSLI GKGCMC 1 cut(s) 157
BstUI CGCG 2 cut(s) 239, 1086
BstV1I GCAGC 4 cut(s) 686, 1016, 1065, 1088
BstV2I GAAGAC 3 cut(s) 777, 819, 1035
BstX2I RGATCY 2 cut(s) 1212, 1258
BstYI RGATCY 2 cut(s) 1212, 1258
BtgI CCRYGG 1 cut(s) 123
BtsCI GGATG 4 cut(s) 352, 822, 993, 1006
BtsI GCAGTG 1 cut(s) 1104
BtsIMutI CAGTG 2 cut(s) 924, 1104
Cac8I GCNNGC 4 cut(s) 163, 947, 1057, 1139
CfoI GCGC 1 cut(s) 61
Cfr13I GGNCC 1 cut(s) 516
CseI GACGC 1 cut(s) 245
Csp6I GTAC 1 cut(s) 928
CspCI CAANNNNNGTGG 2 cut(s) 547, 582
CviQI GTAC 1 cut(s) 928
DdeI CTNAG 4 cut(s) 527, 655, 1262, 1394
DpnI GATC 3 cut(s) 482, 1214, 1260
DpnII GATC 3 cut(s) 480, 1212, 1258
DraI TTTAAA 1 cut(s) 1206
DriI GACNNNNNGTC 1 cut(s) 778
Eam1104I CTCTTC 3 cut(s) 9, 316, 1146
Eam1105I GACNNNNNGTC 1 cut(s) 778
EarI CTCTTC 3 cut(s) 9, 316, 1146
Eco130I CCWWGG 1 cut(s) 123
Eco47I GGWCC 1 cut(s) 516
Eco57I CTGAAG 1 cut(s) 1047
EcoT14I CCWWGG 1 cut(s) 123
EcoT22I ATGCAT 2 cut(s) 730, 987
ErhI CCWWGG 1 cut(s) 123
FalI AAGNNNNNCTT 2 cut(s) 770, 802
FaqI GGGAC 1 cut(s) 871
FauI CCCGC 1 cut(s) 170
FblI GTMKAC 1 cut(s) 607
Fnu4HI GCNGC 4 cut(s) 675, 1005, 1079, 1102
FokI GGATG 4 cut(s) 359, 809, 980, 1013
Fsp4HI GCNGC 4 cut(s) 675, 1005, 1079, 1102
FspBI CTAG 6 cut(s) 297, 686, 830, 900, 942, 1272
GlaI GCGC 1 cut(s) 60
GluI GCNGC 4 cut(s) 675, 1005, 1079, 1102
GsaI CCCAGC 1 cut(s) 393
GsuI CTGGAG 2 cut(s) 612, 1157
HaeII RGCGCY 1 cut(s) 62
HapII CCGG 1 cut(s) 997
HgaI GACGC 1 cut(s) 245
HhaI GCGC 1 cut(s) 61
Hin6I GCGC 1 cut(s) 59
HinP1I GCGC 1 cut(s) 59
HindIII AAGCTT 2 cut(s) 1320, 1382
HinfI GANTC 7 cut(s) 385, 509, 554, 914, 938, 1132, 1421
HpaII CCGG 1 cut(s) 997
HphI GGTGA 2 cut(s) 424, 475
Hpy166II GTNNAC 5 cut(s) 130, 142, 155, 558, 608
Hpy188I TCNGA 5 cut(s) 502, 528, 644, 827, 1027
Hpy188III TCNNGA 5 cut(s) 233, 463, 856, 1174, 1428
Hpy8I GTNNAC 5 cut(s) 130, 142, 155, 558, 608
Hpy99I CGWCG 1 cut(s) 876
HpyAV CCTTC 3 cut(s) 49, 576, 1091
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 4 cut(s) 527, 655, 1262, 1394
HspAI GCGC 1 cut(s) 59
Kzo9I GATC 3 cut(s) 480, 1212, 1258
LmnI GCTCC 2 cut(s) 271, 400
Lsp1109I GCAGC 4 cut(s) 686, 1016, 1065, 1088
LweI GCATC 6 cut(s) 70, 458, 715, 868, 972, 991
MaeI CTAG 6 cut(s) 297, 686, 830, 900, 942, 1272
MalI GATC 3 cut(s) 482, 1214, 1260
MboI GATC 3 cut(s) 480, 1212, 1258
MflI RGATCY 2 cut(s) 1212, 1258
MhlI GDGCHC 2 cut(s) 157, 640
MluCI AATT 9 cut(s) 169, 217, 251, 282, 291, 1223, 1244, 1346, 1389
MluI ACGCGT 1 cut(s) 237
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 1 cut(s) 850
MnlI CCTC 8 cut(s) 98, 497, 712, 896, 1063, 1116, 1325, 1422
Mph1103I ATGCAT 2 cut(s) 730, 987
MroXI GAANNNNTTC 2 cut(s) 18, 228
MseI TTAA 5 cut(s) 746, 1205, 1239, 1247, 1377
MslI CAYNNNNRTG 2 cut(s) 150, 733
MspCI CTTAAG 2 cut(s) 1238, 1376
MspI CCGG 1 cut(s) 997
MspR9I CCNGG 1 cut(s) 998
Mva1269I GAATGC 1 cut(s) 728
MvnI CGCG 2 cut(s) 239, 1086
MwoI GCNNNNNNNGC 1 cut(s) 623
NciI CCSGG 1 cut(s) 998
NcoI CCATGG 1 cut(s) 123
NdeII GATC 3 cut(s) 480, 1212, 1258
NsiI ATGCAT 2 cut(s) 730, 987
NspV TTCGAA 2 cut(s) 12, 222
PctI GAATGC 1 cut(s) 728
PdmI GAANNNNTTC 2 cut(s) 18, 228
PfeI GAWTC 6 cut(s) 385, 509, 914, 938, 1132, 1421
PfoI TCCNGGA 1 cut(s) 996
PkrI GCNGC 4 cut(s) 676, 1006, 1080, 1103
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PsiI TTATAA 1 cut(s) 338
PspFI CCCAGC 1 cut(s) 389
PspPI GGNCC 1 cut(s) 516
PsuI RGATCY 2 cut(s) 1212, 1258
RsaI GTAC 1 cut(s) 929
RsaNI GTAC 1 cut(s) 928
RseI CAYNNNNRTG 2 cut(s) 150, 733
SaqAI TTAA 5 cut(s) 746, 1205, 1239, 1247, 1377
SatI GCNGC 4 cut(s) 675, 1005, 1079, 1102
Sau3AI GATC 3 cut(s) 480, 1212, 1258
Sau96I GGNCC 1 cut(s) 516
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 1 cut(s) 998
SduI GDGCHC 2 cut(s) 157, 640
SfaNI GCATC 6 cut(s) 70, 458, 715, 868, 972, 991
SfuI TTCGAA 2 cut(s) 12, 222
SinI GGWCC 1 cut(s) 516
SmiMI CAYNNNNRTG 2 cut(s) 150, 733
SmlI CTYRAG 3 cut(s) 1238, 1376, 1415
SmoI CTYRAG 3 cut(s) 1238, 1376, 1415
SpeI ACTAGT 1 cut(s) 1271
Sse9I AATT 9 cut(s) 169, 217, 251, 282, 291, 1223, 1244, 1346, 1389
SsiI CCGC 3 cut(s) 163, 659, 1086
SspI AATATT 1 cut(s) 1015
SspMI CTAG 6 cut(s) 297, 686, 830, 900, 942, 1272
StyD4I CCNGG 1 cut(s) 996
StyI CCWWGG 1 cut(s) 123
TaqI TCGA 5 cut(s) 12, 78, 222, 871, 891
TasI AATT 9 cut(s) 169, 217, 251, 282, 291, 1223, 1244, 1346, 1389
TfiI GAWTC 6 cut(s) 385, 509, 914, 938, 1132, 1421
Tru1I TTAA 5 cut(s) 746, 1205, 1239, 1247, 1377
Tru9I TTAA 5 cut(s) 746, 1205, 1239, 1247, 1377
TscAI CASTG 2 cut(s) 924, 1111
TseI GCWGC 4 cut(s) 674, 1004, 1078, 1101
TspDTI ATGAA 7 cut(s) 56, 60, 68, 87, 170, 783, 1110
TspGWI ACGGA 1 cut(s) 1207
TspRI CASTG 2 cut(s) 924, 1111
Vha464I CTTAAG 2 cut(s) 1238, 1376
VneI GTGCAC 1 cut(s) 153
VpaK11BI GGWCC 1 cut(s) 516
XapI RAATTY 2 cut(s) 1223, 1389
XmiI GTMKAC 1 cut(s) 607
XmnI GAANNNNTTC 2 cut(s) 18, 228
XspI CTAG 6 cut(s) 297, 686, 830, 900, 942, 1272
Zsp2I ATGCAT 2 cut(s) 730, 987
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.