FvH4_3g44670

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37202775 .. 37208481
5707 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g44670.t1

Sequence Viewer

Length: 3297 bp
ATGGCCTCCTCTGCATCTCTTCCTTCGTCCGCTGAATCATCATCATCAGCTCGTCCGTGCAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTACATCTGAATTATATAAGCGGCTGGATGAACAGAGAGGAATCAAAACATTCATGGATGATCGAGATCTTGAAGTAGGGGATGCTATATCTCCCACTCTACTAAAAGCAATTGAAGAATCAAGGTTTGCAATTGTTGTCCTTTCTCCAAACTATGCCTCTTCTACTTGGTGTTTGGAGGAACTTGCGAAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTCTTCCACTTTTCTATAATGTCGAGCCTACTGATGTAAGACATCAAAAGGGAAGTTTTGAAGAAGCTTTCAGCAAATACGAGAGGCGTAGATCAGAGAAGGTCCAGCAGTGGAGAGATGCTTTAAACAAAGTGGCCAGTTTCACTGGGTGGCATACAAAGAATTATAAGACTGATAGAGAACTTGCGGAGGAAATTGTGGATTCTGTGTGCAACAGAATACGACCTTTTGAAATTGACTTGACATTGGCCACCGGAGATTTTGGAGAGTTTGAAGCAACAAAAAAAGCCATGGGTCAGGTTATGAAGGTGCTTAAAGATGACAACGTCACTGCCGTTGGGGTGTACGGAATGGCAGGCGTAGGCAAGACAACCCTGGTAAAACATGTTAGTGCAGATGCCCGAGAAAAAGGGATTTTTCATTATGCGGTTATGGCTGTTCTATCCCAAAGCCCAGACATAGCAAAGATTCAAGGCACATTGGCAGATATGTTGGGCGTTAAATTGGAGGGAGAGACAGAATTTGGAAGAGCTGTTAGATTGAAGAGAGAGATAATGAGAAGAGAAAAAATCCTTATAATCTTGGACGATGTTTGGCAGATAATAGAGTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAAAGTTCAAGTCCAAAGTGCTATGCACTACAAGGAAATCAAATGTCTGTCATGCCATGGAGTGCCAAGAAAAGATCACCCTCAACATTCTCTCAGAAAGAGATTCTTGGAACTTGTTTCTGAGAAAAGCAGGAACAATTCCTCTCGAGTCCCAGAACGTGGCGAGGAAGGTAGCTGGAGAATGTAAGGGTCTACCGATTGCATTAATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCAGGAGGAATGGGAGAAGGCAGCTAAACGACTAGAGCAGTCACAATATGTCAATCCCAATTATGAGGAAGATGAGGAAAATGCATTCAGATGTATACGATTAAGCTATGATTACTTGAAAAATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACTGTAACATCGAAATAGAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCCCAAACAATGGAAGAAGCCAGAGGAAAATTAGTTACAGTGATCAAGTACCTGAAAGGTTCTAGCTTACTTTTGGATAGTGAGGAAGACGGATGTGTTAAGATGCATGATGTCATCCGGGATACAGCCCTGAATATTGCACAATCTGAAGATGGGCATCGGTTTTTTGTGAAAGCTGGCGTTGGTTTAAAGGGTTGGCTGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACGAAATTCGCAAGCTACCCGAAGACGAGGTGGTATGTCCAAATCTCCAGATTTTATTACTAAACGGGAATAAGGAACTAAGTGAGATCCCCGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAGATAAATGCGGCGAATTGATTGACATTTCTGTTGTGGGGAAACTTAAGAAGCTTGAAATTCTTAGGATGAGAGACTGTCCTGGGACCCAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAACCGCTCTTGGAGGGGACGAATGGTTACAACTCCATCTAAAGTGATATCAAAGTTGCAAAAATTAGAAGAATTGTACTGTGGAATTGAGGACCGGTGGTGTGAACCAACAGAAAGAGCCAATCTTATTACGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATATTGCATGTTGGCTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACATTTGTATAAGTGTTGGCAGATGGCCCTACACTCCTCAATACAAACAACGAGATCGTAATTCAAGAACCTTGTTTCTTACAGGCACCATCTGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATGACAGCTGCAAAGGGATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTTGCTAGGTCACAACACAAGTTGAAAGAGTTGATGAACACAACAAGACGAGTTGAAACAGGACCCGTGTTTGAGAATTTGGAAGAGTTGCATCTGAGAGATCTGATCCACCTGGAGGAGTTGTGTGTTGGTGCGTTACCACCTGGGTCTTTCTCAAATCTCAAGGTGTTTCATGTGTTTTCTGTTAAGATTTTGAGCAGTGTATCAAAATTTGTACAGAGACTAACAAATCTGGAGAAACTAGATTTAAACTATATGGAGAAATTGGAATATGTGTTCAGATGTAGATGGTTCAATCCAGAACAAGCGAAACTGAGAGAGATGCATTTGTTGAGTCTAATAGCACTAAGAAGAATATGTTATGGTCCGGCTCCACGTGCAATGTTCCGGAGTCTTAAAATTTTGACCATTTACGGTTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTCAGTGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGTTTCTACCAAAACTGAAGAACTTGGTTTTGAAGGAACTTCCTTTGTTGTATGGTGCAAGTGGTGGCGTTGATATTGTGTGTCCTTCATTGGAACACTTGATGGTGGTGGATTGCCCCAAGCTTTTATTTTCAACCTCTTCCGATCTCTTCGAAGCCTTCAAAAGCAGGAACCAATTTTCATTTTCAGCCTCCACTTCTAACCCAGTCCAACTCGATGATCCACAACTATACCAAACTCTACGTGGCAGAGCATCTGGTTTCCGATCATGA

Protein Analysis

1099

Amino Acids

125.18

Weight (kDa)

6.87

Isoelectric Point (pI)

48.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 183 1.6e-54 TIR domain
TIR_2 PF13676 24 - 120 3.5e-17 TIR domain
NB-ARC PF00931 205 - 370 4.1e-30 NB-ARC domain
LRR_8 PF13855 578 - 635 2.4e-10 Leucine rich repeat
LRR_14 PF23598 583 - 711 1.8e-18 Leucine-rich repeat region
LRR_RPS2 PF23247 854 - 994 7.8e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 935 - 1050 5.9e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 498, 908
AasI GACNNNNNNGTC 1 cut(s) 1990
AccB1I GGYRCC 1 cut(s) 2342
AccB7I CCANNNNNTGG 2 cut(s) 1135, 1456
AccBSI CCGCTC 1 cut(s) 2043
AccI GTMKAC 2 cut(s) 1168, 1309
AccII CGCG 2 cut(s) 1421, 1653
AccIII TCCGGA 1 cut(s) 2871
AciI CCGC 8 cut(s) 30, 130, 518, 758, 1653, 1908, 2041, 2378
AclWI GGATC 5 cut(s) 1774, 1789, 1833, 2584, 3239
AcoI YGGCCR 2 cut(s) 465, 579
AcsI RAATTY 8 cut(s) 330, 851, 1698, 1956, 2194, 2560, 2693, 2883
AcuI CTGAAG 2 cut(s) 1614, 3062
AcvI CACGTG 1 cut(s) 2861
AdeI CACNNNGTG 1 cut(s) 480
AfaI GTAC 6 cut(s) 677, 1418, 1496, 2114, 2353, 2700
AfiI CCNNNNNNNGG 5 cut(s) 1135, 1456, 2022, 2599, 2989
AflII CTTAAG 1 cut(s) 1943
AflIII ACRYGT 1 cut(s) 715
AgeI ACCGGT 1 cut(s) 2130
AhdI GACNNNNNGTC 1 cut(s) 1348
AhlI ACTAGT 1 cut(s) 1838
AjnI CCWGG 5 cut(s) 705, 1215, 1978, 2595, 2626
AloI GAACNNNNNNTCC 2 cut(s) 2744, 2776
Alw26I GTCTC 4 cut(s) 839, 1874, 1965, 2698
AlwI GGATC 5 cut(s) 1774, 1789, 1833, 2584, 3239
AlwNI CAGNNNCTG 1 cut(s) 1804
Ama87I CYCGRG 2 cut(s) 732, 1121
Aor13HI TCCGGA 1 cut(s) 2871
AoxI GGCC 4 cut(s) 3, 465, 579, 2282
ApeKI GCWGC 6 cut(s) 1235, 1645, 1668, 2415, 2908, 2911
ApoI RAATTY 8 cut(s) 330, 851, 1698, 1956, 2194, 2560, 2693, 2883
AseI ATTAAT 1 cut(s) 1181
AsiGI ACCGGT 1 cut(s) 2130
Asp700I GAANNNNTTC 1 cut(s) 2964
AspS9I GGNCC 8 cut(s) 89, 433, 1213, 1983, 2128, 2283, 2546, 2849
AsuC2I CCSGG 1 cut(s) 1565
AsuHPI GGTGA 3 cut(s) 1045, 1867, 2392
AsuII TTCGAA 1 cut(s) 3177
AvaI CYCGRG 2 cut(s) 732, 1121
AvaII GGWCC 7 cut(s) 89, 433, 1213, 1983, 2128, 2546, 2849
BaeGI GKGCMC 1 cut(s) 1201
BalI TGGCCA 2 cut(s) 467, 581
BanI GGYRCC 1 cut(s) 2342
BarI GAAGNNNNNNTAC 2 cut(s) 94, 126
BbrPI CACGTG 1 cut(s) 2861
BbsI GAAGAC 7 cut(s) 1347, 1389, 1410, 1539, 1722, 2967, 3029
BbvI GCAGC 6 cut(s) 1247, 1632, 1655, 2402, 2895, 2898
BccI CCATC 6 cut(s) 1592, 2080, 2274, 2354, 2766, 3121
BceAI ACGGC 1 cut(s) 650
BciT130I CCWGG 5 cut(s) 707, 1217, 1980, 2597, 2628
BciVI GTATCC 1 cut(s) 1561
BclI TGATCA 1 cut(s) 1488
BcnI CCSGG 1 cut(s) 1565
BcoDI GTCTC 4 cut(s) 839, 1874, 1965, 2698
BcuI ACTAGT 1 cut(s) 1838
BfaI CTAG 6 cut(s) 1247, 1509, 1839, 2402, 2490, 2726
BfrI CTTAAG 1 cut(s) 1943
BfuI GTATCC 1 cut(s) 1561
BglII AGATCT 3 cut(s) 175, 300, 2584
BisI GCNGC 8 cut(s) 131, 1236, 1646, 1669, 1909, 2416, 2909, 2912
BlsI GCNGC 8 cut(s) 132, 1237, 1647, 1670, 1910, 2417, 2910, 2913
Bme1390I CCNGG 6 cut(s) 707, 1217, 1565, 1980, 2597, 2628
Bme18I GGWCC 7 cut(s) 89, 433, 1213, 1983, 2128, 2546, 2849
BmeRI GACNNNNNGTC 1 cut(s) 1348
BmeT110I CYCGRG 2 cut(s) 732, 1121
BmgT120I GGNCC 8 cut(s) 89, 433, 1213, 1983, 2128, 2283, 2546, 2849
BmiI GGNNCC 7 cut(s) 90, 1984, 1985, 2344, 2548, 2856, 3197
BmrFI CCNGG 6 cut(s) 707, 1217, 1565, 1980, 2597, 2628
BmrI ACTGGG 2 cut(s) 486, 3224
BmuI ACTGGG 2 cut(s) 486, 3224
BpiI GAAGAC 7 cut(s) 1347, 1389, 1410, 1539, 1722, 2967, 3029
BpmI CTGGAG 4 cut(s) 1173, 1724, 2618, 2738
Bpu14I TTCGAA 1 cut(s) 3177
BpuEI CTTGAG 2 cut(s) 2456, 2630
BpuMI CCSGG 1 cut(s) 1565
BsaAI YACGTR 2 cut(s) 2861, 3269
BsaBI GATNNNNATC 2 cut(s) 174, 1602
BsaJI CCNNGG 6 cut(s) 621, 705, 1032, 1979, 2452, 2627
BsaWI WCCGGW 4 cut(s) 584, 1413, 2130, 2871
BsaXI ACNNNNNCTCC 4 cut(s) 2591, 2621, 3011, 3041
Bsc4I CCNNNNNNNGG 5 cut(s) 1135, 1456, 2022, 2599, 2989
Bse118I RCCGGY 1 cut(s) 2130
Bse1I ACTGG 3 cut(s) 468, 481, 3230
Bse3DI GCAATG 1 cut(s) 2871
Bse8I GATNNNNATC 2 cut(s) 174, 1602
BseAI TCCGGA 1 cut(s) 2871
BseBI CCWGG 5 cut(s) 707, 1217, 1980, 2597, 2628
BseDI CCNNGG 6 cut(s) 621, 705, 1032, 1979, 2452, 2627
BseGI GGATG 9 cut(s) 142, 172, 196, 1544, 1560, 1971, 2038, 2220, 2430
BseJI GATNNNNATC 2 cut(s) 174, 1602
BseLI CCNNNNNNNGG 5 cut(s) 1135, 1456, 2022, 2599, 2989
BseMI GCAATG 1 cut(s) 2871
BseMII CTCAG 5 cut(s) 1083, 1088, 2570, 2789, 2954
BseNI ACTGG 3 cut(s) 468, 481, 3230
BseRI GAGGAG 3 cut(s) 1862, 2283, 2615
BseSI GKGCMC 1 cut(s) 1201
BseXI GCAGC 6 cut(s) 1247, 1632, 1655, 2402, 2895, 2898
BseYI CCCAGC 1 cut(s) 959
BsgI GTGCAG 2 cut(s) 744, 1654
Bsh1236I CGCG 2 cut(s) 1421, 1653
Bsh1285I CGRYCG 1 cut(s) 1425
BshFI GGCC 4 cut(s) 5, 467, 581, 2284
BshNI GGYRCC 1 cut(s) 2342
BshTI ACCGGT 1 cut(s) 2130
BsiEI CGRYCG 1 cut(s) 1425
BsiHKCI CYCGRG 2 cut(s) 732, 1121
BsiSI CCGG 6 cut(s) 585, 1414, 1564, 2131, 2852, 2872
BslFI GGGAC 3 cut(s) 1111, 1996, 2067
BslI CCNNNNNNNGG 5 cut(s) 1135, 1456, 2022, 2599, 2989
BsmAI GTCTC 4 cut(s) 839, 1874, 1965, 2698
BsmFI GGGAC 3 cut(s) 1111, 1996, 2067
BsmI GAATGC 1 cut(s) 1298
BsnI GGCC 4 cut(s) 5, 467, 581, 2284
BsoBI CYCGRG 2 cut(s) 732, 1121
Bsp119I TTCGAA 1 cut(s) 3177
Bsp1286I GDGCHC 1 cut(s) 1201
Bsp13I TCCGGA 1 cut(s) 2871
Bsp1407I TGTACA 1 cut(s) 2698
Bsp19I CCATGG 3 cut(s) 621, 1032, 2452
BspACI CCGC 8 cut(s) 30, 130, 518, 758, 1653, 1908, 2041, 2378
BspANI GGCC 4 cut(s) 5, 467, 581, 2284
BspCNI CTCAG 5 cut(s) 1082, 1089, 2571, 2790, 2953
BspEI TCCGGA 1 cut(s) 2871
BspFNI CGCG 2 cut(s) 1421, 1653
BspHI TCATGA 1 cut(s) 3293
BspLI GGNNCC 7 cut(s) 90, 1984, 1985, 2344, 2548, 2856, 3197
BspPI GGATC 5 cut(s) 1774, 1789, 1833, 2584, 3239
BspQI GCTCTTC 1 cut(s) 853
BspT104I TTCGAA 1 cut(s) 3177
BspT107I GGYRCC 1 cut(s) 2342
BspTI CTTAAG 1 cut(s) 1943
BsrBI CCGCTC 1 cut(s) 2043
BsrDI GCAATG 1 cut(s) 2871
BsrFI RCCGGY 1 cut(s) 2130
BsrGI TGTACA 1 cut(s) 2698
BsrI ACTGG 3 cut(s) 468, 481, 3230
BssAI RCCGGY 1 cut(s) 2130
BssECI CCNNGG 6 cut(s) 621, 705, 1032, 1979, 2452, 2627
BssNAI GTATAC 1 cut(s) 1310
BssT1I CCWWGG 3 cut(s) 621, 1032, 2452
Bst1107I GTATAC 1 cut(s) 1310
Bst2UI CCWGG 5 cut(s) 707, 1217, 1980, 2597, 2628
Bst4CI ACNGT 6 cut(s) 1388, 1486, 1976, 2117, 2485, 2900
Bst6I CTCTTC 8 cut(s) 24, 274, 853, 869, 886, 2562, 3169, 3179
BstAFI CTTAAG 1 cut(s) 1943
BstAUI TGTACA 1 cut(s) 2698
BstBAI YACGTR 2 cut(s) 2861, 3269
BstBI TTCGAA 1 cut(s) 3177
BstC8I GCNNGC 4 cut(s) 688, 1624, 1706, 2906
BstDSI CCRYGG 3 cut(s) 621, 1032, 2452
BstF5I GGATG 9 cut(s) 142, 172, 196, 1544, 1560, 1971, 2038, 2220, 2430
BstFNI CGCG 2 cut(s) 1421, 1653
BstMAI GTCTC 4 cut(s) 839, 1874, 1965, 2698
BstMCI CGRYCG 1 cut(s) 1425
BstMWI GCNNNNNNNGC 4 cut(s) 11, 764, 1184, 2861
BstNI CCWGG 5 cut(s) 707, 1217, 1980, 2597, 2628
BstNSI RCATGY 2 cut(s) 719, 2213
BstSCI CCNGG 6 cut(s) 705, 1215, 1563, 1978, 2595, 2626
BstSLI GKGCMC 1 cut(s) 1201
BstUI CGCG 2 cut(s) 1421, 1653
BstV1I GCAGC 6 cut(s) 1247, 1632, 1655, 2402, 2895, 2898
BstV2I GAAGAC 7 cut(s) 1347, 1389, 1410, 1539, 1722, 2967, 3029
BstX2I RGATCY 5 cut(s) 175, 300, 1779, 1825, 2584
BstYI RGATCY 5 cut(s) 175, 300, 1779, 1825, 2584
BstZ17I GTATAC 1 cut(s) 1310
BsuI GTATCC 1 cut(s) 1561
BsuRI GGCC 4 cut(s) 5, 467, 581, 2284
BtgI CCRYGG 3 cut(s) 621, 1032, 2452
BtsCI GGATG 9 cut(s) 142, 172, 196, 1544, 1560, 1971, 2038, 2220, 2430
BtsI GCAGTG 5 cut(s) 446, 660, 1671, 2689, 3015
BtsIMutI CAGTG 8 cut(s) 446, 474, 660, 1491, 1671, 2689, 2948, 3015
Cac8I GCNNGC 4 cut(s) 688, 1624, 1706, 2906
CaiI CAGNNNCTG 1 cut(s) 1804
CciI TCATGA 1 cut(s) 3293
Cfr10I RCCGGY 1 cut(s) 2130
Cfr13I GGNCC 8 cut(s) 89, 433, 1213, 1983, 2128, 2283, 2546, 2849
Csp6I GTAC 6 cut(s) 676, 1417, 1495, 2113, 2352, 2699
CspAI ACCGGT 1 cut(s) 2130
CviQI GTAC 6 cut(s) 676, 1417, 1495, 2113, 2352, 2699
DraI TTTAAA 3 cut(s) 456, 1635, 2733
DraIII CACNNNGTG 1 cut(s) 480
DrdI GACNNNNNNGTC 1 cut(s) 1990
DriI GACNNNNNGTC 1 cut(s) 1348
DseDI GACNNNNNNGTC 1 cut(s) 1990
EaeI YGGCCR 2 cut(s) 465, 579
Eam1104I CTCTTC 8 cut(s) 24, 274, 853, 869, 886, 2562, 3169, 3179
Eam1105I GACNNNNNGTC 1 cut(s) 1348
EarI CTCTTC 8 cut(s) 24, 274, 853, 869, 886, 2562, 3169, 3179
Eco130I CCWWGG 3 cut(s) 621, 1032, 2452
Eco32I GATATC 1 cut(s) 2085
Eco47I GGWCC 7 cut(s) 89, 433, 1213, 1983, 2128, 2546, 2849
Eco57I CTGAAG 2 cut(s) 1614, 3062
Eco72I CACGTG 1 cut(s) 2861
Eco88I CYCGRG 2 cut(s) 732, 1121
EcoO109I RGGNCCY 3 cut(s) 89, 1983, 2546
EcoRI GAATTC 1 cut(s) 330
EcoRII CCWGG 5 cut(s) 705, 1215, 1978, 2595, 2626
EcoRV GATATC 1 cut(s) 2085
EcoT14I CCWWGG 3 cut(s) 621, 1032, 2452
EcoT22I ATGCAT 3 cut(s) 1300, 1554, 2811
ErhI CCWWGG 3 cut(s) 621, 1032, 2452
FalI AAGNNNNNCTT 6 cut(s) 1066, 1098, 1340, 1372, 2952, 2984
FaqI GGGAC 3 cut(s) 1111, 1996, 2067
FbaI TGATCA 1 cut(s) 1488
FblI GTMKAC 2 cut(s) 1168, 1309
Fnu4HI GCNGC 8 cut(s) 131, 1236, 1646, 1669, 1909, 2416, 2909, 2912
FokI GGATG 9 cut(s) 149, 179, 203, 1547, 1551, 1978, 2045, 2207, 2437
Fsp4HI GCNGC 8 cut(s) 131, 1236, 1646, 1669, 1909, 2416, 2909, 2912
FspBI CTAG 6 cut(s) 1247, 1509, 1839, 2402, 2490, 2726
GluI GCNGC 8 cut(s) 131, 1236, 1646, 1669, 1909, 2416, 2909, 2912
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 4 cut(s) 1173, 1724, 2618, 2738
HaeIII GGCC 4 cut(s) 5, 467, 581, 2284
HapII CCGG 6 cut(s) 585, 1414, 1564, 2131, 2852, 2872
HincII GTYRAC 1 cut(s) 2038
HindII GTYRAC 1 cut(s) 2038
HindIII AAGCTT 4 cut(s) 396, 1887, 1949, 3146
HpaII CCGG 6 cut(s) 585, 1414, 1564, 2131, 2852, 2872
HphI GGTGA 3 cut(s) 1045, 1867, 2392
Hpy166II GTNNAC 6 cut(s) 676, 1169, 1310, 2038, 2141, 3013
Hpy8I GTNNAC 6 cut(s) 676, 1169, 1310, 2038, 2141, 3013
HpyCH4III ACNGT 6 cut(s) 1388, 1486, 1976, 2117, 2485, 2900
HpyCH4IV ACGT 6 cut(s) 657, 1134, 2168, 2860, 3026, 3268
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 764, 1184, 2861
HpySE526I ACGT 6 cut(s) 657, 1134, 2168, 2860, 3026, 3268
KflI GGGWCCC 1 cut(s) 1983
Kpn2I TCCGGA 1 cut(s) 2871
Ksp22I TGATCA 1 cut(s) 1488
LguI GCTCTTC 1 cut(s) 853
LmnI GCTCC 2 cut(s) 970, 2860
Lsp1109I GCAGC 6 cut(s) 1247, 1632, 1655, 2402, 2895, 2898
MaeI CTAG 6 cut(s) 1247, 1509, 1839, 2402, 2490, 2726
MaeII ACGT 6 cut(s) 657, 1134, 2168, 2860, 3026, 3268
MbiI CCGCTC 1 cut(s) 2043
MfeI CAATTG 4 cut(s) 219, 240, 1988, 2954
MflI RGATCY 5 cut(s) 175, 300, 1779, 1825, 2584
MhlI GDGCHC 1 cut(s) 1201
MlsI TGGCCA 2 cut(s) 467, 581
MluNI TGGCCA 2 cut(s) 467, 581
MlyI GAGTC 3 cut(s) 1133, 2827, 2884
MmeI TCCRAC 2 cut(s) 2970, 3259
Mox20I TGGCCA 2 cut(s) 467, 581
Mph1103I ATGCAT 3 cut(s) 1300, 1554, 2811
MroI TCCGGA 1 cut(s) 2871
MroXI GAANNNNTTC 1 cut(s) 2964
MscI TGGCCA 2 cut(s) 467, 581
MslI CAYNNNNRTG 3 cut(s) 720, 1303, 2438
Msp20I TGGCCA 2 cut(s) 467, 581
MspA1I CMGCKG 2 cut(s) 32, 2415
MspCI CTTAAG 1 cut(s) 1943
MspI CCGG 6 cut(s) 585, 1414, 1564, 2131, 2852, 2872
MspR9I CCNGG 6 cut(s) 707, 1217, 1565, 1980, 2597, 2628
MunI CAATTG 4 cut(s) 219, 240, 1988, 2954
Mva1269I GAATGC 1 cut(s) 1298
MvaI CCWGG 5 cut(s) 707, 1217, 1980, 2597, 2628
MvnI CGCG 2 cut(s) 1421, 1653
MwoI GCNNNNNNNGC 4 cut(s) 11, 764, 1184, 2861
NciI CCSGG 1 cut(s) 1565
NcoI CCATGG 3 cut(s) 621, 1032, 2452
NlaIV GGNNCC 7 cut(s) 90, 1984, 1985, 2344, 2548, 2856, 3197
NmuCI GTSAC 5 cut(s) 658, 1254, 2380, 2429, 2493
NsiI ATGCAT 3 cut(s) 1300, 1554, 2811
NspI RCATGY 2 cut(s) 719, 2213
NspV TTCGAA 1 cut(s) 3177
PaeR7I CTCGAG 1 cut(s) 1121
PagI TCATGA 1 cut(s) 3293
PciI ACATGT 1 cut(s) 715
PciSI GCTCTTC 1 cut(s) 853
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 1 cut(s) 1298
PdmI GAANNNNTTC 1 cut(s) 2964
PfeI GAWTC 8 cut(s) 35, 150, 227, 533, 799, 955, 1079, 1801
PflFI GACNNNGTC 1 cut(s) 656
PflMI CCANNNNNTGG 2 cut(s) 1135, 1456
PfoI TCCNGGA 1 cut(s) 1563
PinAI ACCGGT 1 cut(s) 2130
PkrI GCNGC 8 cut(s) 132, 1237, 1647, 1670, 1910, 2417, 2910, 2913
Ple19I CGATCG 1 cut(s) 1425
PleI GAGTC 3 cut(s) 1132, 2826, 2883
PmaCI CACGTG 1 cut(s) 2861
PmlI CACGTG 1 cut(s) 2861
PpsI GAGTC 3 cut(s) 1132, 2826, 2883
Ppu21I YACGTR 2 cut(s) 2861, 3269
PpuMI RGGWCCY 3 cut(s) 89, 1983, 2546
PscI ACATGT 1 cut(s) 715
PshBI ATTAAT 1 cut(s) 1181
PsiI TTATAA 2 cut(s) 498, 908
Psp5II RGGWCCY 3 cut(s) 89, 1983, 2546
Psp6I CCWGG 5 cut(s) 705, 1215, 1978, 2595, 2626
PspCI CACGTG 1 cut(s) 2861
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 5 cut(s) 705, 1215, 1978, 2595, 2626
PspN4I GGNNCC 7 cut(s) 90, 1984, 1985, 2344, 2548, 2856, 3197
PspPI GGNCC 8 cut(s) 89, 433, 1213, 1983, 2128, 2283, 2546, 2849
PspPPI RGGWCCY 3 cut(s) 89, 1983, 2546
PstNI CAGNNNCTG 1 cut(s) 1804
PsuI RGATCY 5 cut(s) 175, 300, 1779, 1825, 2584
PsyI GACNNNGTC 1 cut(s) 656
PvuI CGATCG 1 cut(s) 1425
PvuII CAGCTG 1 cut(s) 2415
RsaI GTAC 6 cut(s) 677, 1418, 1496, 2114, 2353, 2700
RsaNI GTAC 6 cut(s) 676, 1417, 1495, 2113, 2352, 2699
RseI CAYNNNNRTG 3 cut(s) 720, 1303, 2438
SapI GCTCTTC 1 cut(s) 853
SatI GCNGC 8 cut(s) 131, 1236, 1646, 1669, 1909, 2416, 2909, 2912
Sau96I GGNCC 8 cut(s) 89, 433, 1213, 1983, 2128, 2283, 2546, 2849
SchI GAGTC 3 cut(s) 1133, 2827, 2884
ScrFI CCNGG 6 cut(s) 707, 1217, 1565, 1980, 2597, 2628
SduI GDGCHC 1 cut(s) 1201
Sfr274I CTCGAG 1 cut(s) 1121
SfuI TTCGAA 1 cut(s) 3177
SinI GGWCC 7 cut(s) 89, 433, 1213, 1983, 2128, 2546, 2849
SlaI CTCGAG 1 cut(s) 1121
SmiMI CAYNNNNRTG 3 cut(s) 720, 1303, 2438
SmlI CTYRAG 4 cut(s) 1121, 1943, 2471, 2645
SmoI CTYRAG 4 cut(s) 1121, 1943, 2471, 2645
SpeI ACTAGT 1 cut(s) 1838
SsiI CCGC 8 cut(s) 30, 130, 518, 758, 1653, 1908, 2041, 2378
SspI AATATT 2 cut(s) 1582, 2205
SspMI CTAG 6 cut(s) 1247, 1509, 1839, 2402, 2490, 2726
StyD4I CCNGG 6 cut(s) 705, 1215, 1563, 1978, 2595, 2626
StyI CCWWGG 3 cut(s) 621, 1032, 2452
TaaI ACNGT 6 cut(s) 1388, 1486, 1976, 2117, 2485, 2900
TaiI ACGT 6 cut(s) 660, 1137, 2171, 2863, 3029, 3271
TaqI TCGA 9 cut(s) 100, 172, 354, 1122, 1395, 1441, 1994, 3177, 3240
TatI WGTACW 2 cut(s) 2112, 2698
TauI GCSGC 2 cut(s) 133, 1911
TfiI GAWTC 8 cut(s) 35, 150, 227, 533, 799, 955, 1079, 1801
TscAI CASTG 8 cut(s) 446, 481, 667, 1491, 1678, 2689, 2948, 3015
TseFI GTSAC 5 cut(s) 658, 1254, 2380, 2429, 2493
TseI GCWGC 6 cut(s) 1235, 1645, 1668, 2415, 2908, 2911
Tsp45I GTSAC 5 cut(s) 658, 1254, 2380, 2429, 2493
TspGWI ACGGA 4 cut(s) 45, 693, 1551, 1837
TspRI CASTG 8 cut(s) 446, 481, 667, 1491, 1678, 2689, 2948, 3015
Tth111I GACNNNGTC 1 cut(s) 656
Van91I CCANNNNNTGG 2 cut(s) 1135, 1456
Vha464I CTTAAG 1 cut(s) 1943
VpaK11BI GGWCC 7 cut(s) 89, 433, 1213, 1983, 2128, 2546, 2849
VspI ATTAAT 1 cut(s) 1181
XapI RAATTY 8 cut(s) 330, 851, 1698, 1956, 2194, 2560, 2693, 2883
XceI RCATGY 2 cut(s) 719, 2213
XcmI CCANNNNNNNNNTGG 1 cut(s) 3266
XhoI CTCGAG 1 cut(s) 1121
XmiI GTMKAC 2 cut(s) 1168, 1309
XmnI GAANNNNTTC 1 cut(s) 2964
XspI CTAG 6 cut(s) 1247, 1509, 1839, 2402, 2490, 2726
Zsp2I ATGCAT 3 cut(s) 1300, 1554, 2811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.