RchiOBHm_Chr5g0079441

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
85116020 .. 85122146
6127 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35384

Sequence Viewer

Length: 3378 bp
ATGGATGCCTCTTCATCTGTTCCTTCATCATCAGCAGCTCGTCCGTGGATGTATGATTTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTACATCCGATCTATACGATCGGCTGCAAAAGAGAGGAATTAAAACATTCATGGATGACCAAGATCTTCAAGTAGGGGATGGGATTTCTTCCACTCTCATAGCAGCAATTGAAAAATCAAGGTTTGCAATCGTTGTTCTGTCGGAAAACTATGCTGATTCTGCTTGGTGTTTGGAGGAACTTACAAAGATTTGTGAGTGCAAGGAAGAAAGCAAAGGAATTTTTCCACTTTTCTATAATGTGGACCCCTCTGATGTTCGGCACCGGAAGAGGAGTTTCGGAGAAGCTTTAAATAAGCATGGAAGCTCTGGGCGACACGCATCAGAGAAGGTGCAGCGGTGGAATGATGCTTTATACAAAGTGGCTAGTTTCTCTGGGTGGCATACAAAGGATTACAAGACTGATAGAGAACTTGTGGTGGAAATTGTGGATTTTGTGTGCAGTAAAATACAACTGGATATTCCAATGTCTACCGGAGATTTTGAAGAGTTTGAAGCAACAAAAAAAGCCATGGATGATGTTGTGAAGGCGCTTAAAGATGACAAGGTCACTGCCATTGGGGTCTACGGAATGGGCGGTGTCGGCAAGACAACCATGGTAAAACATGTTGCTGCACAATCCAAAATAGGTGGGATTTTTGATTATGTGATTATTGGTGTGGTATCCCAGAGCCCTGACTTGACAAAGATTCAAGGCACATTGGCAGATACATTGGGCATTACATTGGCGGGAGAGACAGAATTTGGAAGAGCTGCTAGATTGCATACGGAGATAATGAGAAGAGAAAAGATCCTTATAATCTTGGACGATGTTTGGATGATAACAGACTTGTCAAAGATAGGGATTCCCAGCTTCAAGGAGCTTCAAGAGTGCAATTCCAAAGTCGTTCTCACCACACGGAAAGGGAATGTCTGTTATGTCATGAAGTGCCAAGCAAGCATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGACCTTGTTTGTGAGAAACACAAGTAGGTCTTTTGAATCCCCCGATTTTTATAATGTAGCGAGGAAGGTAGCTGGAGAATGCGGGGGTCTACCCATTGCACTGATAGCAGTTGCAAGGGCACTCGGAGATAAAGAACTGGTGGAATGGAAAAGAGCAGCTAAACGACTAGAGAAGTCGCAATTTGCCAACCCTGACCATGACGAAGATGCTTTCAAATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATGTTTCTTGCTGTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGAATTGTTCCGGTATGCAATTGGGAAAGGATTGTTTCGTGATTACGACACAATATATGAAGCCAGAGGAATCGCACATTCAGTGGTCAAGCACCTGAAAGATTCTAGCTTGCTTTTGGAGGGTGAGTTCGGTGCAGTATTCAGATGTGAAAGTGTAAAGATGCATGATGCCGTCCGGGATACAGCCATGAAAATTGCAAAACGTGAAGATGGGCATGGGTTTTTGGTGAAAGCTGGATGGGGTTTAGATCATTGGTCATTTCGATCACATGAAGACTACTCTGCAATCTCACTAATGGGTAATAACATTCGCGGTCCACCCGAAGAGTCGGTAAGTCCAAAACTCCAGATTTTATCACTACAATTCAATACTGAATTAAATGAGATCCCAGAGACCTTTTTCCCGAGTTCCAATGAATTAATGCTCTTGGATCTTGGCGACACTAGTATTTCCTCTCTACCCCAATCTTTCAGTCTCCTTACCAACCTCCAAGCTTTGTTTTTAGATTCTTGCGAAAACTTGATTGACATTTCTATAGTGGGAAAACTGACGAAGCTTGAAATTCTTACTATGAGGGAGTATCCTGGGGGGAGATTGCCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGGAAGTCAGTGCAACGCCATTTGGAGAAGCTTTAGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGCTGGGGTGTGGAATTGTGCGCTGGGGGTGTAAAATTGATGGGTGTGGACTTGGGCACTGGCAGTGTACAGATGAGGACTGGCGGCGTAAAATTGAGGGACAAGGAGGAGAAACCAATATTGGGTTTGATGAGTTAGCTGGTTTATCATGTTTGAAAGTTTTGCAGGTTTCCATATCTTATGCTTCATACATCCCTAAAAATGTTGAGGCCGAACCGAAATGGGTTTACTTTGACATACATATTGGCGAATGGGAGGAATATTCATTCAATCAACAAGATCATAATTCAAGATCCTTGTATCTTAGGGGTTATACCATAAGTACCTTGCCTGATTGGTTTATCAACGTGGTGACAGAGAAAACAGAGAAGCTAAAGTATGAAAACTGGGAAGGGATGACGAGTGACATTCTTATGGAATATGACCATGGGAGGTTACACGAACTCGAGTATCTCCGTGTAGATCATGATTGGTGTTCTCATTACGATGCGTACGTGTTGATGAATACAACAAGACGAGTTCAAGAAGGACCAGTGTTTGAGAAGTTGAAAGAGTTGCATCTGACATTTCTGGACCACCTGGAGGCGTTGTGTGTTGGTGAGTTACCACCTGGGTCTTTCTCGAATCTCAAGGTATTGCAGGTGTATTGTTGTGATCGCATAAAGAATGTATCAAAATTCGTACAGAGACTACCAAATCTGGAGAAACTAGATTTAAACAATATGCGCCAATTGGAATATGTGTTTAAATGCGAAGGGTTCGAGCCAGAACAATCCAAACTGAGAGAGATGCATTTGTTGGGTGAAAATTATGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCCAGAGTCTTAAAAGTTTGATCTTTTACAACTGTGAGGAGCTGGTAAGTCTGTTCGCATCTGATGTAGCTGAGTGTCTTGTTCAATTGGAAGACCTTTTTGTAGAGCGGTGCCGTTGGTTGTATAGAGTAATTGAAGCAGTGAACAACAAGCAGATTACGGTTCTTCGAAATTTGAAGAACATGGTTTTGAAGGACCTTCCTATGTTGTATAGTGCAAGTTGTACTGTTGATATTGAGTGTCCTTTATTGGAACGCTTGTTTGTGGTGAATTGCCCCCACATTTCATTTTCAACCTCTGCTTCTGCCTACTTTGGCAGCACGAAACCAGTCCAACTCAATGATTCACAACTGTACGATTTGCTGCGTGACGGAACATCTCCTTTCATAGGGGAGTACACGGAATGGTCCCGCTGA

Protein Analysis

1125

Amino Acids

127.83

Weight (kDa)

5.37

Isoelectric Point (pI)

47.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 18 - 181 1.1e-44 TIR domain
TIR_2 PF13676 21 - 115 2.9e-16 TIR domain
NB-ARC PF00931 200 - 362 5.1e-29 NB-ARC domain
LRR_8 PF13855 575 - 633 7.7e-08 Leucine rich repeat
LRR_14 PF23598 577 - 707 4.9e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 886 - 1026 1.4e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 965 - 1081 3e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 893, 1122
AarI CACCTGC 1 cut(s) 2740
Acc36I ACCTGC 2 cut(s) 2266, 2740
AccB1I GGYRCC 2 cut(s) 357, 3072
AccBSI CCGCTC 1 cut(s) 3070
AccI GTMKAC 3 cut(s) 566, 660, 1159
AccII CGCG 2 cut(s) 1698, 2955
AciI CCGC 8 cut(s) 433, 672, 824, 1152, 1698, 2194, 3070, 3373
AclWI GGATC 4 cut(s) 880, 1765, 1824, 2397
AcsI RAATTY 6 cut(s) 315, 836, 1947, 1999, 2786, 3133
AfaI GTAC 8 cut(s) 2108, 2179, 2434, 2603, 2793, 3187, 3317, 3359
AfiI CCNNNNNNNGG 4 cut(s) 2013, 2232, 2692, 3350
AflIII ACRYGT 3 cut(s) 700, 2604, 2953
AhdI GACNNNNNGTC 1 cut(s) 1330
AhlI ACTAGT 1 cut(s) 1829
AjnI CCWGG 3 cut(s) 1969, 2688, 2719
AjuI GAANNNNNNNTTGG 4 cut(s) 1020, 1052, 2214, 2246
AloI GAACNNNNNNTCC 2 cut(s) 1496, 1528
Alw26I GTCTC 4 cut(s) 824, 1772, 1865, 2791
AlwI GGATC 4 cut(s) 880, 1765, 1824, 2397
Ama87I CYCGRG 2 cut(s) 1789, 2555
AoxI GGCC 1 cut(s) 2319
ApeKI GCWGC 9 cut(s) 35, 121, 200, 430, 707, 848, 1226, 3279, 3325
ApoI RAATTY 6 cut(s) 315, 836, 1947, 1999, 2786, 3133
AseI ATTAAT 1 cut(s) 1805
AspLEI GCGC 3 cut(s) 628, 2133, 2838
AspS9I GGNCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
AsuC2I CCSGG 1 cut(s) 1562
AsuHPI GGTGA 7 cut(s) 979, 1520, 1624, 2473, 2720, 2924, 3241
AsuII TTCGAA 1 cut(s) 3130
AvaI CYCGRG 2 cut(s) 1789, 2555
AvaII GGWCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
BaeGI GKGCMC 2 cut(s) 1192, 2169
BaeI ACNNNNGTAYC 2 cut(s) 2543, 2576
BanI GGYRCC 2 cut(s) 357, 3072
BanII GRGCYC 1 cut(s) 770
BarI GAAGNNNNNNTAC 2 cut(s) 82, 114
BbsI GAAGAC 3 cut(s) 1371, 1665, 3060
BbvI GCAGC 9 cut(s) 47, 108, 212, 442, 694, 835, 1238, 3291, 3312
BccI CCATC 5 cut(s) 170, 1589, 1617, 2074, 2144
BceAI ACGGC 2 cut(s) 1541, 3060
BciT130I CCWGG 3 cut(s) 1971, 2690, 2721
BciVI GTATCC 3 cut(s) 769, 1558, 1977
BcnI CCSGG 1 cut(s) 1562
BcoDI GTCTC 4 cut(s) 824, 1772, 1865, 2791
BcuI ACTAGT 1 cut(s) 1829
BfaI CTAG 6 cut(s) 462, 852, 1238, 1491, 1830, 2819
BfmI CTRYAG 1 cut(s) 1920
BfoI RGCGCY 1 cut(s) 629
BfuAI ACCTGC 2 cut(s) 2266, 2740
BfuI GTATCC 3 cut(s) 769, 1558, 1977
BglII AGATCT 1 cut(s) 160
Bme1390I CCNGG 4 cut(s) 1562, 1971, 2690, 2721
Bme18I GGWCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
BmeRI GACNNNNNGTC 1 cut(s) 1330
BmeT110I CYCGRG 2 cut(s) 1789, 2555
BmgT120I GGNCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
BmiI GGNNCC 5 cut(s) 78, 342, 359, 3074, 3371
BmrFI CCNGG 4 cut(s) 1562, 1971, 2690, 2721
BmrI ACTGGG 1 cut(s) 2506
BmsI GCATC 9 cut(s) 425, 433, 1267, 1536, 1543, 2587, 2677, 2889, 3029
BmuI ACTGGG 1 cut(s) 2506
BpiI GAAGAC 3 cut(s) 1371, 1665, 3060
BpmI CTGGAG 4 cut(s) 1164, 1715, 2711, 2831
Bpu14I TTCGAA 1 cut(s) 3130
BpuEI CTTGAG 1 cut(s) 2723
BpuMI CCSGG 1 cut(s) 1562
BsaAI YACGTR 1 cut(s) 2605
BsaI GGTCTC 1 cut(s) 1772
BsaJI CCNNGG 6 cut(s) 44, 606, 690, 1970, 2536, 2720
BsaWI WCCGGW 3 cut(s) 360, 569, 1395
BsaXI ACNNNNNCTCC 4 cut(s) 1496, 1526, 2684, 2714
Bsc4I CCNNNNNNNGG 4 cut(s) 2013, 2232, 2692, 3350
Bse1I ACTGG 7 cut(s) 555, 1212, 2174, 2195, 2501, 2642, 3290
Bse3DI GCAATG 1 cut(s) 1164
BseBI CCWGG 3 cut(s) 1971, 2690, 2721
BseDI CCNNGG 6 cut(s) 44, 606, 690, 1970, 2536, 2720
BseLI CCNNNNNNNGG 4 cut(s) 2013, 2232, 2692, 3350
BseMI GCAATG 1 cut(s) 1164
BseMII CTCAG 2 cut(s) 2882, 3024
BseNI ACTGG 7 cut(s) 555, 1212, 2174, 2195, 2501, 2642, 3290
BseRI GAGGAG 3 cut(s) 382, 2232, 3014
BseSI GKGCMC 2 cut(s) 1192, 2169
BseXI GCAGC 9 cut(s) 47, 108, 212, 442, 694, 835, 1238, 3291, 3312
BseYI CCCAGC 3 cut(s) 944, 2113, 2133
BsgI GTGCAG 4 cut(s) 449, 556, 693, 1539
Bsh1236I CGCG 2 cut(s) 1698, 2955
Bsh1285I CGRYCG 1 cut(s) 118
BshFI GGCC 1 cut(s) 2321
BshNI GGYRCC 2 cut(s) 357, 3072
BsiEI CGRYCG 1 cut(s) 118
BsiHKCI CYCGRG 2 cut(s) 1789, 2555
BsiSI CCGG 4 cut(s) 361, 570, 1396, 1561
BsiWI CGTACG 1 cut(s) 2601
BslFI GGGAC 2 cut(s) 2223, 3355
BslI CCNNNNNNNGG 4 cut(s) 2013, 2232, 2692, 3350
BsmAI GTCTC 4 cut(s) 824, 1772, 1865, 2791
BsmFI GGGAC 2 cut(s) 2223, 3355
BsmI GAATGC 2 cut(s) 1035, 1154
BsnI GGCC 1 cut(s) 2321
Bso31I GGTCTC 1 cut(s) 1772
BsoBI CYCGRG 2 cut(s) 1789, 2555
Bsp119I TTCGAA 1 cut(s) 3130
Bsp1286I GDGCHC 3 cut(s) 770, 1192, 2169
Bsp1407I TGTACA 2 cut(s) 2106, 2177
Bsp19I CCATGG 3 cut(s) 606, 690, 2536
BspACI CCGC 8 cut(s) 433, 672, 824, 1152, 1698, 2194, 3070, 3373
BspANI GGCC 1 cut(s) 2321
BspCNI CTCAG 2 cut(s) 2883, 3025
BspFNI CGCG 2 cut(s) 1698, 2955
BspHI TCATGA 2 cut(s) 1017, 2575
BspLI GGNNCC 5 cut(s) 78, 342, 359, 3074, 3371
BspMI ACCTGC 2 cut(s) 2266, 2740
BspPI GGATC 4 cut(s) 880, 1765, 1824, 2397
BspQI GCTCTTC 2 cut(s) 838, 2921
BspT104I TTCGAA 1 cut(s) 3130
BspT107I GGYRCC 2 cut(s) 357, 3072
BspTNI GGTCTC 1 cut(s) 1772
BsrBI CCGCTC 1 cut(s) 3070
BsrDI GCAATG 1 cut(s) 1164
BsrGI TGTACA 2 cut(s) 2106, 2177
BsrI ACTGG 7 cut(s) 555, 1212, 2174, 2195, 2501, 2642, 3290
BssECI CCNNGG 6 cut(s) 44, 606, 690, 1970, 2536, 2720
BssT1I CCWWGG 3 cut(s) 606, 690, 2536
Bst2UI CCWGG 3 cut(s) 1971, 2690, 2721
Bst4CI ACNGT 4 cut(s) 2996, 3124, 3190, 3315
Bst6I CTCTTC 7 cut(s) 16, 359, 576, 838, 871, 1704, 2921
BstAUI TGTACA 2 cut(s) 2106, 2177
BstBAI YACGTR 1 cut(s) 2605
BstBI TTCGAA 1 cut(s) 3130
BstC8I GCNNGC 2 cut(s) 1033, 1496
BstDEI CTNAG 4 cut(s) 2012, 2414, 2891, 3033
BstDSI CCRYGG 4 cut(s) 44, 606, 690, 2536
BstENI CCTNNNNNAGG 1 cut(s) 3348
BstFNI CGCG 2 cut(s) 1698, 2955
BstH2I RGCGCY 1 cut(s) 629
BstHHI GCGC 3 cut(s) 628, 2133, 2838
BstMAI GTCTC 4 cut(s) 824, 1772, 1865, 2791
BstMCI CGRYCG 1 cut(s) 118
BstMWI GCNNNNNNNGC 4 cut(s) 257, 678, 1032, 1175
BstNI CCWGG 3 cut(s) 1971, 2690, 2721
BstNSI RCATGY 2 cut(s) 704, 2113
BstSCI CCNGG 4 cut(s) 1560, 1969, 2688, 2719
BstSFI CTRYAG 1 cut(s) 1920
BstSLI GKGCMC 2 cut(s) 1192, 2169
BstUI CGCG 2 cut(s) 1698, 2955
BstV1I GCAGC 9 cut(s) 47, 108, 212, 442, 694, 835, 1238, 3291, 3312
BstV2I GAAGAC 3 cut(s) 1371, 1665, 3060
BstX2I RGATCY 5 cut(s) 160, 885, 1770, 1816, 2402
BstYI RGATCY 5 cut(s) 160, 885, 1770, 1816, 2402
BsuI GTATCC 3 cut(s) 769, 1558, 1977
BsuRI GGCC 1 cut(s) 2321
BtgI CCRYGG 4 cut(s) 44, 606, 690, 2536
BtsI GCAGTG 3 cut(s) 645, 2180, 3108
BtsIMutI CAGTG 8 cut(s) 645, 1169, 1473, 2035, 2167, 2180, 2649, 3108
BveI ACCTGC 2 cut(s) 2266, 2740
Cac8I GCNNGC 2 cut(s) 1033, 1496
CciI TCATGA 2 cut(s) 1017, 2575
CfoI GCGC 3 cut(s) 628, 2133, 2838
Cfr13I GGNCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
Csp6I GTAC 8 cut(s) 2107, 2178, 2433, 2602, 2792, 3186, 3316, 3358
CspCI CAANNNNNGTGG 2 cut(s) 706, 741
CviQI GTAC 8 cut(s) 2107, 2178, 2433, 2602, 2792, 3186, 3316, 3358
DdeI CTNAG 4 cut(s) 2012, 2414, 2891, 3033
DraI TTTAAA 3 cut(s) 387, 2826, 2857
DriI GACNNNNNGTC 1 cut(s) 1330
Eam1104I CTCTTC 7 cut(s) 16, 359, 576, 838, 871, 1704, 2921
Eam1105I GACNNNNNGTC 1 cut(s) 1330
EarI CTCTTC 7 cut(s) 16, 359, 576, 838, 871, 1704, 2921
Eco130I CCWWGG 3 cut(s) 606, 690, 2536
Eco24I GRGCYC 1 cut(s) 770
Eco31I GGTCTC 1 cut(s) 1772
Eco32I GATATC 1 cut(s) 2079
Eco47I GGWCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
Eco88I CYCGRG 2 cut(s) 1789, 2555
EcoNI CCTNNNNNAGG 1 cut(s) 3348
EcoO109I RGGNCCY 2 cut(s) 77, 3157
EcoRII CCWGG 3 cut(s) 1969, 2688, 2719
EcoRV GATATC 1 cut(s) 2079
EcoT14I CCWWGG 3 cut(s) 606, 690, 2536
EcoT22I ATGCAT 2 cut(s) 1551, 2904
EcoT38I GRGCYC 1 cut(s) 770
ErhI CCWWGG 3 cut(s) 606, 690, 2536
FalI AAGNNNNNCTT 2 cut(s) 1084, 1116
FaqI GGGAC 2 cut(s) 2223, 3355
FauI CCCGC 2 cut(s) 817, 1145
FblI GTMKAC 3 cut(s) 566, 660, 1159
FriOI GRGCYC 1 cut(s) 770
FspBI CTAG 6 cut(s) 462, 852, 1238, 1491, 1830, 2819
GlaI GCGC 3 cut(s) 627, 2132, 2837
GsaI CCCAGC 3 cut(s) 948, 2117, 2137
GsuI CTGGAG 4 cut(s) 1164, 1715, 2711, 2831
HaeII RGCGCY 1 cut(s) 629
HaeIII GGCC 1 cut(s) 2321
HapII CCGG 4 cut(s) 361, 570, 1396, 1561
HhaI GCGC 3 cut(s) 628, 2133, 2838
Hin6I GCGC 3 cut(s) 626, 2131, 2836
HinP1I GCGC 3 cut(s) 626, 2131, 2836
HindIII AAGCTT 4 cut(s) 381, 1878, 1940, 2049
HpaII CCGG 4 cut(s) 361, 570, 1396, 1561
HphI GGTGA 7 cut(s) 979, 1520, 1624, 2473, 2720, 2924, 3241
HpyCH4III ACNGT 4 cut(s) 2996, 3124, 3190, 3315
HpyCH4IV ACGT 3 cut(s) 1588, 2457, 2604
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 678, 1032, 1175
HpyF3I CTNAG 4 cut(s) 2012, 2414, 2891, 3033
HpySE526I ACGT 3 cut(s) 1588, 2457, 2604
HspAI GCGC 3 cut(s) 626, 2131, 2836
LguI GCTCTTC 2 cut(s) 838, 2921
LmnI GCTCC 2 cut(s) 955, 3001
Lsp1109I GCAGC 9 cut(s) 47, 108, 212, 442, 694, 835, 1238, 3291, 3312
LweI GCATC 9 cut(s) 425, 433, 1267, 1536, 1543, 2587, 2677, 2889, 3029
MaeI CTAG 6 cut(s) 462, 852, 1238, 1491, 1830, 2819
MaeII ACGT 3 cut(s) 1588, 2457, 2604
MaeIII GTNAC 7 cut(s) 643, 2056, 2461, 2513, 2544, 2712, 3329
MbiI CCGCTC 1 cut(s) 3070
MfeI CAATTG 4 cut(s) 204, 1404, 2840, 3047
MflI RGATCY 5 cut(s) 160, 885, 1770, 1816, 2402
MhlI GDGCHC 3 cut(s) 770, 1192, 2169
MluI ACGCGT 1 cut(s) 2953
MlyI GAGTC 2 cut(s) 1721, 2977
MmeI TCCRAC 2 cut(s) 219, 3319
Mph1103I ATGCAT 2 cut(s) 1551, 2904
MseI TTAA 9 cut(s) 138, 386, 630, 1298, 1763, 1805, 2825, 2856, 2973
MslI CAYNNNNRTG 2 cut(s) 2522, 2595
MspA1I CMGCKG 2 cut(s) 433, 3375
MspI CCGG 4 cut(s) 361, 570, 1396, 1561
MspR9I CCNGG 4 cut(s) 1562, 1971, 2690, 2721
MunI CAATTG 4 cut(s) 204, 1404, 2840, 3047
Mva1269I GAATGC 2 cut(s) 1035, 1154
MvaI CCWGG 3 cut(s) 1971, 2690, 2721
MvnI CGCG 2 cut(s) 1698, 2955
MwoI GCNNNNNNNGC 4 cut(s) 257, 678, 1032, 1175
NciI CCSGG 1 cut(s) 1562
NcoI CCATGG 3 cut(s) 606, 690, 2536
NlaIV GGNNCC 5 cut(s) 78, 342, 359, 3074, 3371
NmeAIII GCCGAG 1 cut(s) 2010
NmuCI GTSAC 5 cut(s) 643, 2056, 2461, 2513, 3329
NsiI ATGCAT 2 cut(s) 1551, 2904
NspI RCATGY 2 cut(s) 704, 2113
NspV TTCGAA 1 cut(s) 3130
PaeR7I CTCGAG 1 cut(s) 2555
PagI TCATGA 2 cut(s) 1017, 2575
PaqCI CACCTGC 1 cut(s) 2740
PciI ACATGT 1 cut(s) 700
PciSI GCTCTTC 2 cut(s) 838, 2921
PcsI WCGNNNNNNNCGW 1 cut(s) 2601
PctI GAATGC 2 cut(s) 1035, 1154
Pfl23II CGTACG 1 cut(s) 2601
PflFI GACNNNGTC 1 cut(s) 641
PfoI TCCNGGA 1 cut(s) 1560
Ple19I CGATCG 1 cut(s) 118
PleI GAGTC 2 cut(s) 1720, 2976
PpsI GAGTC 2 cut(s) 1720, 2976
Ppu21I YACGTR 1 cut(s) 2605
PpuMI RGGWCCY 2 cut(s) 77, 3157
PscI ACATGT 1 cut(s) 700
PshBI ATTAAT 1 cut(s) 1805
PsiI TTATAA 2 cut(s) 893, 1122
Psp5II RGGWCCY 2 cut(s) 77, 3157
Psp6I CCWGG 3 cut(s) 1969, 2688, 2719
PspFI CCCAGC 3 cut(s) 944, 2113, 2133
PspGI CCWGG 3 cut(s) 1969, 2688, 2719
PspLI CGTACG 1 cut(s) 2601
PspN4I GGNNCC 5 cut(s) 78, 342, 359, 3074, 3371
PspPI GGNCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
PspPPI RGGWCCY 2 cut(s) 77, 3157
PspXI VCTCGAGB 1 cut(s) 2555
PsuI RGATCY 5 cut(s) 160, 885, 1770, 1816, 2402
PsyI GACNNNGTC 1 cut(s) 641
PvuI CGATCG 1 cut(s) 118
RsaI GTAC 8 cut(s) 2108, 2179, 2434, 2603, 2793, 3187, 3317, 3359
RsaNI GTAC 8 cut(s) 2107, 2178, 2433, 2602, 2792, 3186, 3316, 3358
RseI CAYNNNNRTG 2 cut(s) 2522, 2595
SapI GCTCTTC 2 cut(s) 838, 2921
SaqAI TTAA 9 cut(s) 138, 386, 630, 1298, 1763, 1805, 2825, 2856, 2973
Sau96I GGNCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
SchI GAGTC 2 cut(s) 1721, 2977
ScrFI CCNGG 4 cut(s) 1562, 1971, 2690, 2721
SduI GDGCHC 3 cut(s) 770, 1192, 2169
SfaNI GCATC 9 cut(s) 425, 433, 1267, 1536, 1543, 2587, 2677, 2889, 3029
SfcI CTRYAG 1 cut(s) 1920
Sfr274I CTCGAG 1 cut(s) 2555
SfuI TTCGAA 1 cut(s) 3130
SinI GGWCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
SlaI CTCGAG 1 cut(s) 2555
SmiMI CAYNNNNRTG 2 cut(s) 2522, 2595
SmlI CTYRAG 2 cut(s) 2555, 2738
SmoI CTYRAG 2 cut(s) 2555, 2738
SpeI ACTAGT 1 cut(s) 1829
SsiI CCGC 8 cut(s) 433, 672, 824, 1152, 1698, 2194, 3070, 3373
SspI AATATT 2 cut(s) 2230, 2372
SspMI CTAG 6 cut(s) 462, 852, 1238, 1491, 1830, 2819
StyD4I CCNGG 4 cut(s) 1560, 1969, 2688, 2719
StyI CCWWGG 3 cut(s) 606, 690, 2536
TaaI ACNGT 4 cut(s) 2996, 3124, 3190, 3315
TaiI ACGT 3 cut(s) 1591, 2460, 2607
TaqI TCGA 6 cut(s) 88, 1648, 2556, 2732, 2871, 3130
TatI WGTACW 4 cut(s) 2106, 2177, 3185, 3357
TauI GCSGC 1 cut(s) 2197
Tru1I TTAA 9 cut(s) 138, 386, 630, 1298, 1763, 1805, 2825, 2856, 2973
Tru9I TTAA 9 cut(s) 138, 386, 630, 1298, 1763, 1805, 2825, 2856, 2973
TscAI CASTG 8 cut(s) 652, 1176, 1473, 2035, 2174, 2180, 2649, 3108
TseFI GTSAC 5 cut(s) 643, 2056, 2461, 2513, 3329
TseI GCWGC 9 cut(s) 35, 121, 200, 430, 707, 848, 1226, 3279, 3325
Tsp45I GTSAC 5 cut(s) 643, 2056, 2461, 2513, 3329
TspGWI ACGGA 7 cut(s) 33, 678, 878, 1009, 2555, 3348, 3377
TspRI CASTG 8 cut(s) 652, 1176, 1473, 2035, 2174, 2180, 2649, 3108
Tth111I GACNNNGTC 1 cut(s) 641
VpaK11BI GGWCC 9 cut(s) 77, 340, 1071, 1700, 2639, 2683, 2942, 3157, 3369
VspI ATTAAT 1 cut(s) 1805
XagI CCTNNNNNAGG 1 cut(s) 3348
XapI RAATTY 6 cut(s) 315, 836, 1947, 1999, 2786, 3133
XceI RCATGY 2 cut(s) 704, 2113
XhoI CTCGAG 1 cut(s) 2555
XmiI GTMKAC 3 cut(s) 566, 660, 1159
XspI CTAG 6 cut(s) 462, 852, 1238, 1491, 1830, 2819
Zsp2I ATGCAT 2 cut(s) 1551, 2904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.