Rw5G048250

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000379
Physical Location & Seq
Reverse (-)
83800027 .. 83804232
4206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048250.1

Sequence Viewer

Length: 3627 bp
ATGGAGTTCTTCGAGATGTCGTTGGCGATTATTGTGGTAATTGTGACATGGATCGGTGCCATAGCTACTATTAAGTGGATATGGACTCTACTGAAGCATTGGTTCGGTTACATGGTTGAAAGGCGTGAGCCAGTATCGCAGGATAATCAAGAACTGATTGACATTGCTACTGAGCAAGTAAGGCCCGAGAGAGAAGAAGAAGAAGAAAAGATCTGGGATCTCCACGACTCTCCTGATCAGGGAAACGATGGCACCACTTCGGCCGCCGTGCCAGTTGCTTCTTCCTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGGATTACAGTCGACATACAGGATCGACTTAATAGGAGTGGAATTAAAACGTTCATGGATGATCGAGATCTTGAAGTAGGGAATGTTATTAGTCCCGCTCTCTTAACAGCAATCAGAGAATCAAAGTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTTTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTCTGCATGGAAGACAACAGAATTTTGCCACTTTTTTATCATGTTGATCCTACTGATGTTCGATATCAGAAGAGGAGCTTCGAAGAAGCTTTCTCTAAGCATGAAACCTCTGGGCGACATGAATCAGAGAAGGTGAAGCAATGGAAAGCTGCTTTAAACAAAGTGGCCAACATCTCTGGGTGGAATACAAATGATTATAAGACTCACAAAGAACTTGTTGACATCATTGTGGAATCTCTCCGCAGTAAAGTACCACCTGATGCAATTGAATCCACAGGAGATTTCCAAGCATATGAAGCAACAACACAAGCCGTGAATGAGGTTATGAAGGCGCTAACAGATAACGAGATCAGTGCCGTTGGTGTCTACGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAAACATGTAGCTGCAAAAGCCTGCAAAAACGGTACTTTTAACCGTTGGATTATCGTTGCCATATCCCAAAACCCCGACTTTGGAAAAATTCAAGGCACATTGGCAGATTTCTTGGGCGTTAAATTGGACAAGGAGACAGAAGATGGAAGAGCGGCTGCGCTGCATAAGGAAATAATGAGAAGCGGAAGACTCCTGATAATCTTGGACGATGTTTGGAACATAATAGAATTGTCAGCCATAGGAATTTCCAGCTACGAGGAGCTTCAAAAGTGCAATTCCAAAGTCGTACTCACCACACGGAGAAGGAATGTCTGTCATGTCATGAAGTGCCAAGCAAGCATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGACCTTGTTTCTGAGAAATGCAGGAATAAATCCTCTTCAGTCCACCACATTTGAGAATGTGGCGAGGAGGGTAGCTGTAGAATGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCTGGAGGAATGGGAAAAGGCAGCTGAACGACTAGAGAAGTCGCGATCTCCCAATCCTAACCATGAGGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAACATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATTCCCAGAAGACCATGAAATCAAAACAGAAGACTTGTTCAGGTATGCGATCGGAAAAAGGTTGTTTCGAGATGCCGAAACAACGGTAGAAGCCAGAGGAATAGTAGTTTCAGTGGTCAAGTACTTGAAAGATTCTAGCTTGCTTATGGATAGCGAGAAAAATGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCAGAATCTGAAAATGGGTTTTGGGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGAGGCCACGCGGATTACATGCAGGCTGCACTGCTATTTCACTGATGAGGAACGAAATTCGCAAGCTACCTGAAGAGGAGTTGGTATGTCCACATCTCCAGATTTTATTACTAAACCGGAATGATGATTTAGATGAGATCCCAGAAAAGTTCATCCAAAAGCTGAATGAATTAAGGCTCTTGGATCTTAGCGACACTAGTATTTCCGTGTTACCGCAATCATTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTCTCGCAAGAAATTTATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGAAACTGTCCCGGGAGCGGGGGACTTTCGCGTTCAGGATGTGAAAGTCCCGAGGAGAGGGTATTGCCGAGAGAAATAGGACATTTGAGCAATCTAAGGATTTTGGATGTCAATCGCTCTTGGGCAAGAGCCTCCGTGAGTGGTGTGGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTTGAGGAAGCCAATATTTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATAGTCCAGCTTGGCATATCTGATGCAAAATACATCCCTAAAAATGTTGAGGCCAAACCAGATTGGGATTACTTTTATATAAGTATCGTCGGCAGACGCAACTCCGGTGCATACGGAAGCGGAGATCATAATTCAAGATCCTTGCATTTTAGTGGAGCAACCATAAGTATCTTACCTGATTGGTTTATCAACGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATGACTGCTGCAAAGGGCTGAGTGACATTCTTATGGAATATGACTATGGGAGGTTACATAAAGTCAAGCATCTCACAGTAAGGGAGAGTTATCAAGGGCAGAGTTCCTCCCACTTGAAAGAGTTGATGAACACAACAAGACGAGTTCAAGAAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGTCAATGCTGTTACAACTTGATGAGTTGTGTGTTGGTGAGTTTCCACCTGGGTCTTTCTCCAATCTCAAGGTATTACATATGGGTGGTTGTATTAGGTTGAAGAATGTATTAAAATTGGTACAGATATTACCAAATCTGGAGAAACTAGATTTGAATTGTATGCAATTGGATTATGTGTTTGGATGCGAAGGATTTGACCCAAAACAATCAAAACTGAGACAGGTGAATTTGCTTAGTCTAGATGCACTAAGAAGCATATGTAATGGTCCTCCCCCACGTGCAGTGTTCCAGAGTCTTAAACGTTTGTTCATTTACAATTGCGAGTTGCTGCAAACTTTGTTCGCATCTGACGTAGCCGAGTGTCTTGATCAATTGGAAGACCTTTTTGTAGAGCAGTGCCCTTTGTTGGATAGAGTAATTGAAGCAGTGAACAAGGAGAAGACGGTTCTTCCAAAATTGAAGAACTTGGTTTTGAAGAAACTTCCTATGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGCCCCTGGTTTTCATTTTCAACTTCTTCCGATCTCTTCGAATCCTTCTTCGAATCCTTCGGAAACATGAACCATAATATTTCATTTTCAACCCCGGCTTCTGACTACTTTGGCAGCACGAACCCAGTCAAACTCAATGATCCACAACTGTACGAGTTCCTATATCTTAGTACATCTGGTTTCTAA

Protein Analysis

1208

Amino Acids

136.56

Weight (kDa)

5.49

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 105 - 268 1.8e-45 TIR domain
TIR_2 PF13676 108 - 202 7.2e-14 TIR domain
NB-ARC PF00931 287 - 452 1.3e-29 NB-ARC domain
LRR_8 PF13855 658 - 715 2e-08 Leucine rich repeat
LRR_14 PF23598 659 - 775 1.6e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 956 - 1095 1.3e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB1I GGYRCC 2 cut(s) 56, 251
AccBSI CCGCTC 3 cut(s) 455, 1109, 2226
AccI GTMKAC 3 cut(s) 369, 921, 1423
AccII CGCG 4 cut(s) 1513, 1893, 2239, 2669
AclI AACGTT 2 cut(s) 407, 3187
AclWI GGATC 8 cut(s) 59, 225, 387, 596, 2014, 2073, 2607, 3575
AcoI YGGCCR 2 cut(s) 261, 720
AcsI RAATTY 9 cut(s) 576, 1044, 1200, 1938, 2153, 2196, 2467, 2866, 3112
AcuI CTGAAG 4 cut(s) 113, 1352, 1974, 2204
AcvI CACGTG 1 cut(s) 3164
AfaI GTAC 9 cut(s) 807, 991, 1245, 1742, 2408, 2417, 3006, 3593, 3613
AfiI CCNNNNNNNGG 6 cut(s) 239, 1037, 2225, 2226, 2265, 3292
AflIII ACRYGT 1 cut(s) 961
AhdI GACNNNNNGTC 1 cut(s) 1594
AhlI ACTAGT 1 cut(s) 2078
AjnI CCWGG 3 cut(s) 1470, 2932, 3446
AjuI GAANNNNNNNTTGG 2 cut(s) 1281, 1313
Alw26I GTCTC 4 cut(s) 560, 1085, 2114, 3097
AlwI GGATC 8 cut(s) 59, 225, 387, 596, 2014, 2073, 2607, 3575
AlwNI CAGNNNCTG 1 cut(s) 1838
Ama87I CYCGRG 3 cut(s) 185, 2219, 2258
AoxI GGCC 5 cut(s) 182, 261, 720, 1886, 2526
ApeKI GCWGC 9 cut(s) 704, 968, 1112, 1117, 1490, 1908, 2706, 3214, 3555
ApoI RAATTY 9 cut(s) 576, 1044, 1200, 1938, 2153, 2196, 2467, 2866, 3112
AspLEI GCGC 2 cut(s) 889, 1117
AspS9I GGNCC 6 cut(s) 183, 341, 1332, 1468, 2852, 3152
AsuC2I CCSGG 4 cut(s) 1811, 2220, 2221, 3536
AsuHPI GGTGA 7 cut(s) 700, 967, 1240, 1867, 2683, 2933, 3121
AsuII TTCGAA 3 cut(s) 636, 3480, 3492
AvaI CYCGRG 3 cut(s) 185, 2219, 2258
AvaII GGWCC 5 cut(s) 341, 1332, 1468, 2852, 3152
BaeGI GKGCMC 2 cut(s) 1456, 3287
BalI TGGCCA 1 cut(s) 722
BanI GGYRCC 2 cut(s) 56, 251
BbrPI CACGTG 1 cut(s) 3164
BbsI GAAGAC 7 cut(s) 573, 1150, 1593, 1635, 1656, 3270, 3332
BbvI GCAGC 9 cut(s) 691, 955, 1099, 1104, 1502, 1895, 2693, 3201, 3567
BccI CCATC 3 cut(s) 242, 1094, 2374
BceAI ACGGC 4 cut(s) 251, 851, 896, 940
BcgI CGANNNNNNTGC 2 cut(s) 890, 924
BciT130I CCWGG 3 cut(s) 1472, 2934, 3448
BciVI GTATCC 1 cut(s) 1807
BclI TGATCA 2 cut(s) 235, 3253
BcnI CCSGG 4 cut(s) 1811, 2220, 2221, 3536
BcoDI GTCTC 4 cut(s) 560, 1085, 2114, 3097
BcuI ACTAGT 1 cut(s) 2078
BfaI CTAG 6 cut(s) 1502, 1755, 2079, 2693, 3032, 3125
BfmI CTRYAG 2 cut(s) 1407, 2169
BfoI RGCGCY 1 cut(s) 890
BfuI GTATCC 1 cut(s) 1807
BglII AGATCT 2 cut(s) 210, 424
BmcAI AGTACT 1 cut(s) 1742
Bme1390I CCNGG 7 cut(s) 1472, 1811, 2220, 2221, 2934, 3448, 3536
Bme18I GGWCC 5 cut(s) 341, 1332, 1468, 2852, 3152
BmeRI GACNNNNNGTC 1 cut(s) 1594
BmeT110I CYCGRG 3 cut(s) 185, 2219, 2258
BmgT120I GGNCC 6 cut(s) 183, 341, 1332, 1468, 2852, 3152
BmiI GGNNCC 3 cut(s) 58, 253, 342
BmrFI CCNGG 7 cut(s) 1472, 1811, 2220, 2221, 2934, 3448, 3536
BmrI ACTGGG 1 cut(s) 3560
BmuI ACTGGG 1 cut(s) 3560
BpiI GAAGAC 7 cut(s) 573, 1150, 1593, 1635, 1656, 3270, 3332
BpmI CTGGAG 3 cut(s) 1493, 1964, 3044
Bpu14I TTCGAA 3 cut(s) 636, 3480, 3492
BpuEI CTTGAG 1 cut(s) 2936
BpuMI CCSGG 4 cut(s) 1811, 2220, 2221, 3536
BsaAI YACGTR 1 cut(s) 3164
BsaBI GATNNNNATC 2 cut(s) 423, 2319
BsaJI CCNNGG 5 cut(s) 2219, 2259, 2933, 3446, 3534
BsaWI WCCGGW 2 cut(s) 1998, 2579
Bsc4I CCNNNNNNNGG 6 cut(s) 239, 1037, 2225, 2226, 2265, 3292
Bse1I ACTGG 4 cut(s) 131, 272, 2855, 3566
Bse3DI GCAATG 2 cut(s) 162, 701
Bse8I GATNNNNATC 2 cut(s) 423, 2319
BseBI CCWGG 3 cut(s) 1472, 2934, 3448
BseDI CCNNGG 5 cut(s) 2219, 2259, 2933, 3446, 3534
BseGI GGATG 8 cut(s) 421, 1790, 1806, 2034, 2252, 2320, 2508, 3074
BseJI GATNNNNATC 2 cut(s) 423, 2319
BseLI CCNNNNNNNGG 6 cut(s) 239, 1037, 2225, 2226, 2265, 3292
BseMI GCAATG 2 cut(s) 162, 701
BseMII CTCAG 4 cut(s) 162, 1334, 2708, 3092
BseNI ACTGG 4 cut(s) 131, 272, 2855, 3566
BseRI GAGGAG 5 cut(s) 643, 1229, 1411, 1973, 2276
BseSI GKGCMC 2 cut(s) 1456, 3287
BseX3I CGGCCG 1 cut(s) 261
BseXI GCAGC 9 cut(s) 691, 955, 1099, 1104, 1502, 1895, 2693, 3201, 3567
BsgI GTGCAG 2 cut(s) 1894, 3186
Bsh1236I CGCG 4 cut(s) 1513, 1893, 2239, 2669
Bsh1285I CGRYCG 2 cut(s) 264, 1671
BshFI GGCC 5 cut(s) 184, 263, 722, 1888, 2528
BshNI GGYRCC 2 cut(s) 56, 251
BsiEI CGRYCG 2 cut(s) 264, 1671
BsiHKCI CYCGRG 3 cut(s) 185, 2219, 2258
BsiSI CCGG 5 cut(s) 1810, 1999, 2220, 2580, 3536
BslFI GGGAC 4 cut(s) 435, 2202, 2241, 2244
BslI CCNNNNNNNGG 6 cut(s) 239, 1037, 2225, 2226, 2265, 3292
BsmAI GTCTC 4 cut(s) 560, 1085, 2114, 3097
BsmFI GGGAC 4 cut(s) 435, 2202, 2241, 2244
BsmI GAATGC 2 cut(s) 1296, 1544
BsnI GGCC 5 cut(s) 184, 263, 722, 1888, 2528
BsoBI CYCGRG 3 cut(s) 185, 2219, 2258
Bsp119I TTCGAA 3 cut(s) 636, 3480, 3492
Bsp1286I GDGCHC 2 cut(s) 1456, 3287
Bsp1407I TGTACA 1 cut(s) 2406
Bsp68I TCGCGA 1 cut(s) 1513
BspANI GGCC 5 cut(s) 184, 263, 722, 1888, 2528
BspCNI CTCAG 4 cut(s) 163, 1335, 2709, 3093
BspFNI CGCG 4 cut(s) 1513, 1893, 2239, 2669
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 3 cut(s) 58, 253, 342
BspPI GGATC 8 cut(s) 59, 225, 387, 596, 2014, 2073, 2607, 3575
BspQI GCTCTTC 1 cut(s) 1099
BspT104I TTCGAA 3 cut(s) 636, 3480, 3492
BspT107I GGYRCC 2 cut(s) 56, 251
BsrBI CCGCTC 3 cut(s) 455, 1109, 2226
BsrDI GCAATG 2 cut(s) 162, 701
BsrGI TGTACA 1 cut(s) 2406
BsrI ACTGG 4 cut(s) 131, 272, 2855, 3566
BssECI CCNNGG 5 cut(s) 2219, 2259, 2933, 3446, 3534
Bst2UI CCWGG 3 cut(s) 1472, 2934, 3448
Bst6I CTCTTC 7 cut(s) 292, 620, 1099, 1371, 1950, 2868, 3482
BstAUI TGTACA 1 cut(s) 2406
BstBAI YACGTR 1 cut(s) 3164
BstBI TTCGAA 3 cut(s) 636, 3480, 3492
BstC8I GCNNGC 6 cut(s) 979, 1294, 1760, 1864, 1906, 1946
BstF5I GGATG 8 cut(s) 421, 1790, 1806, 2034, 2252, 2320, 2508, 3074
BstFNI CGCG 4 cut(s) 1513, 1893, 2239, 2669
BstH2I RGCGCY 1 cut(s) 890
BstHHI GCGC 2 cut(s) 889, 1117
BstMAI GTCTC 4 cut(s) 560, 1085, 2114, 3097
BstMCI CGRYCG 2 cut(s) 264, 1671
BstMWI GCNNNNNNNGC 7 cut(s) 136, 181, 851, 933, 974, 1293, 1439
BstNI CCWGG 3 cut(s) 1472, 2934, 3448
BstNSI RCATGY 2 cut(s) 965, 1904
BstSCI CCNGG 7 cut(s) 1470, 1809, 2218, 2219, 2932, 3446, 3534
BstSFI CTRYAG 2 cut(s) 1407, 2169
BstSLI GKGCMC 2 cut(s) 1456, 3287
BstUI CGCG 4 cut(s) 1513, 1893, 2239, 2669
BstV1I GCAGC 9 cut(s) 691, 955, 1099, 1104, 1502, 1895, 2693, 3201, 3567
BstV2I GAAGAC 7 cut(s) 573, 1150, 1593, 1635, 1656, 3270, 3332
BstX2I RGATCY 6 cut(s) 210, 217, 424, 2019, 2065, 2612
BstYI RGATCY 6 cut(s) 210, 217, 424, 2019, 2065, 2612
BstZI CGGCCG 1 cut(s) 261
BsuI GTATCC 1 cut(s) 1807
BsuRI GGCC 5 cut(s) 184, 263, 722, 1888, 2528
BtsCI GGATG 8 cut(s) 421, 1790, 1806, 2034, 2252, 2320, 2508, 3074
BtsI GCAGTG 4 cut(s) 1911, 3174, 3287, 3318
BtsIMutI CAGTG 8 cut(s) 913, 1737, 1911, 1922, 2862, 3174, 3287, 3318
BtuMI TCGCGA 1 cut(s) 1513
Cac8I GCNNGC 6 cut(s) 979, 1294, 1760, 1864, 1906, 1946
CaiI CAGNNNCTG 1 cut(s) 1838
CciI TCATGA 1 cut(s) 1278
CfoI GCGC 2 cut(s) 889, 1117
Cfr13I GGNCC 6 cut(s) 183, 341, 1332, 1468, 2852, 3152
Cfr9I CCCGGG 1 cut(s) 2219
CseI GACGC 1 cut(s) 2580
Csp6I GTAC 9 cut(s) 806, 990, 1244, 1741, 2407, 2416, 3005, 3592, 3612
CspCI CAANNNNNGTGG 2 cut(s) 1363, 1398
CviQI GTAC 9 cut(s) 806, 990, 1244, 1741, 2407, 2416, 3005, 3592, 3612
DraI TTTAAA 2 cut(s) 711, 1875
DriI GACNNNNNGTC 1 cut(s) 1594
EaeI YGGCCR 2 cut(s) 261, 720
EagI CGGCCG 1 cut(s) 261
Eam1104I CTCTTC 7 cut(s) 292, 620, 1099, 1371, 1950, 2868, 3482
Eam1105I GACNNNNNGTC 1 cut(s) 1594
EarI CTCTTC 7 cut(s) 292, 620, 1099, 1371, 1950, 2868, 3482
EclXI CGGCCG 1 cut(s) 261
Eco32I GATATC 2 cut(s) 620, 2379
Eco47I GGWCC 5 cut(s) 341, 1332, 1468, 2852, 3152
Eco52I CGGCCG 1 cut(s) 261
Eco57I CTGAAG 4 cut(s) 113, 1352, 1974, 2204
Eco72I CACGTG 1 cut(s) 3164
Eco88I CYCGRG 3 cut(s) 185, 2219, 2258
EcoO109I RGGNCCY 2 cut(s) 341, 1468
EcoRII CCWGG 3 cut(s) 1470, 2932, 3446
EcoRV GATATC 2 cut(s) 620, 2379
EcoT22I ATGCAT 2 cut(s) 1546, 1800
FalI AAGNNNNNCTT 4 cut(s) 617, 649, 1586, 1618
FaqI GGGAC 4 cut(s) 435, 2202, 2241, 2244
FauI CCCGC 2 cut(s) 460, 2219
FauNDI CATATG 3 cut(s) 847, 2964, 3143
FbaI TGATCA 2 cut(s) 235, 3253
FblI GTMKAC 3 cut(s) 369, 921, 1423
FokI GGATG 8 cut(s) 428, 1793, 1797, 2021, 2259, 2327, 2495, 3081
FspBI CTAG 6 cut(s) 1502, 1755, 2079, 2693, 3032, 3125
GlaI GCGC 2 cut(s) 888, 1116
GsuI CTGGAG 3 cut(s) 1493, 1964, 3044
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 5 cut(s) 184, 263, 722, 1888, 2528
HapII CCGG 5 cut(s) 1810, 1999, 2220, 2580, 3536
HgaI GACGC 1 cut(s) 2580
HhaI GCGC 2 cut(s) 889, 1117
Hin6I GCGC 2 cut(s) 887, 1115
HinP1I GCGC 2 cut(s) 887, 1115
HincII GTYRAC 2 cut(s) 370, 775
HindII GTYRAC 2 cut(s) 370, 775
HindIII AAGCTT 3 cut(s) 642, 2127, 2189
HpaII CCGG 5 cut(s) 1810, 1999, 2220, 2580, 3536
HphI GGTGA 7 cut(s) 700, 967, 1240, 1867, 2683, 2933, 3121
Hpy166II GTNNAC 8 cut(s) 370, 775, 922, 1374, 1424, 1973, 2416, 3316
Hpy8I GTNNAC 8 cut(s) 370, 775, 922, 1374, 1424, 1973, 2416, 3316
Hpy99I CGWCG 1 cut(s) 2567
HpyAV CCTTC 8 cut(s) 679, 877, 1254, 2843, 3068, 3423, 3496, 3508
HpyCH4IV ACGT 4 cut(s) 407, 3163, 3187, 3237
HpyF10VI GCNNNNNNNGC 7 cut(s) 136, 181, 851, 933, 974, 1293, 1439
HpySE526I ACGT 4 cut(s) 407, 3163, 3187, 3237
HspAI GCGC 2 cut(s) 887, 1115
Ksp22I TGATCA 2 cut(s) 235, 3253
LguI GCTCTTC 1 cut(s) 1099
LmnI GCTCC 4 cut(s) 630, 1216, 2223, 2630
Lsp1109I GCAGC 9 cut(s) 691, 955, 1099, 1104, 1502, 1895, 2693, 3201, 3567
MaeI CTAG 6 cut(s) 1502, 1755, 2079, 2693, 3032, 3125
MaeII ACGT 4 cut(s) 407, 3163, 3187, 3237
MaeIII GTNAC 8 cut(s) 43, 107, 2091, 2356, 2671, 2720, 2751, 2895
MbiI CCGCTC 3 cut(s) 455, 1109, 2226
MfeI CAATTG 4 cut(s) 819, 3050, 3202, 3257
MflI RGATCY 6 cut(s) 210, 217, 424, 2019, 2065, 2612
MhlI GDGCHC 2 cut(s) 1456, 3287
MlsI TGGCCA 1 cut(s) 722
MluNI TGGCCA 1 cut(s) 722
MlyI GAGTC 5 cut(s) 79, 221, 751, 1140, 3187
MmeI TCCRAC 4 cut(s) 919, 983, 1862, 3273
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 2 cut(s) 1546, 1800
MscI TGGCCA 1 cut(s) 722
MslI CAYNNNNRTG 5 cut(s) 782, 1549, 2625, 2729, 2967
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 2 cut(s) 302, 1493
MspI CCGG 5 cut(s) 1810, 1999, 2220, 2580, 3536
MspR9I CCNGG 7 cut(s) 1472, 1811, 2220, 2221, 2934, 3448, 3536
MunI CAATTG 4 cut(s) 819, 3050, 3202, 3257
Mva1269I GAATGC 2 cut(s) 1296, 1544
MvaI CCWGG 3 cut(s) 1472, 2934, 3448
MvnI CGCG 4 cut(s) 1513, 1893, 2239, 2669
MwoI GCNNNNNNNGC 7 cut(s) 136, 181, 851, 933, 974, 1293, 1439
NciI CCSGG 4 cut(s) 1811, 2220, 2221, 3536
NdeI CATATG 3 cut(s) 847, 2964, 3143
NlaIV GGNNCC 3 cut(s) 58, 253, 342
NmeAIII GCCGAG 2 cut(s) 2301, 3268
NmuCI GTSAC 4 cut(s) 43, 2356, 2671, 2720
NruI TCGCGA 1 cut(s) 1513
NsiI ATGCAT 2 cut(s) 1546, 1800
NspI RCATGY 2 cut(s) 965, 1904
NspV TTCGAA 3 cut(s) 636, 3480, 3492
PagI TCATGA 1 cut(s) 1278
PciI ACATGT 1 cut(s) 961
PciSI GCTCTTC 1 cut(s) 1099
PcsI WCGNNNNNNNCGW 1 cut(s) 3234
PctI GAATGC 2 cut(s) 1296, 1544
PfoI TCCNGGA 1 cut(s) 1809
Ple19I CGATCG 1 cut(s) 1671
PleI GAGTC 5 cut(s) 79, 221, 751, 1140, 3186
PmaCI CACGTG 1 cut(s) 3164
PmlI CACGTG 1 cut(s) 3164
PpsI GAGTC 5 cut(s) 79, 221, 751, 1140, 3186
Ppu21I YACGTR 1 cut(s) 3164
PpuMI RGGWCCY 2 cut(s) 341, 1468
PscI ACATGT 1 cut(s) 961
PsiI TTATAA 1 cut(s) 753
Psp1406I AACGTT 2 cut(s) 407, 3187
Psp5II RGGWCCY 2 cut(s) 341, 1468
Psp6I CCWGG 3 cut(s) 1470, 2932, 3446
PspCI CACGTG 1 cut(s) 3164
PspGI CCWGG 3 cut(s) 1470, 2932, 3446
PspN4I GGNNCC 3 cut(s) 58, 253, 342
PspPI GGNCC 6 cut(s) 183, 341, 1332, 1468, 2852, 3152
PspPPI RGGWCCY 2 cut(s) 341, 1468
PstNI CAGNNNCTG 1 cut(s) 1838
PsuI RGATCY 6 cut(s) 210, 217, 424, 2019, 2065, 2612
PvuI CGATCG 1 cut(s) 1671
PvuII CAGCTG 2 cut(s) 302, 1493
RruI TCGCGA 1 cut(s) 1513
RsaI GTAC 9 cut(s) 807, 991, 1245, 1742, 2408, 2417, 3006, 3593, 3613
RsaNI GTAC 9 cut(s) 806, 990, 1244, 1741, 2407, 2416, 3005, 3592, 3612
RseI CAYNNNNRTG 5 cut(s) 782, 1549, 2625, 2729, 2967
SalI GTCGAC 1 cut(s) 368
SapI GCTCTTC 1 cut(s) 1099
Sau96I GGNCC 6 cut(s) 183, 341, 1332, 1468, 2852, 3152
ScaI AGTACT 1 cut(s) 1742
SchI GAGTC 5 cut(s) 79, 221, 751, 1140, 3187
ScrFI CCNGG 7 cut(s) 1472, 1811, 2220, 2221, 2934, 3448, 3536
SduI GDGCHC 2 cut(s) 1456, 3287
SfcI CTRYAG 2 cut(s) 1407, 2169
SfuI TTCGAA 3 cut(s) 636, 3480, 3492
SinI GGWCC 5 cut(s) 341, 1332, 1468, 2852, 3152
SmaI CCCGGG 1 cut(s) 2221
SmiMI CAYNNNNRTG 5 cut(s) 782, 1549, 2625, 2729, 2967
SmlI CTYRAG 1 cut(s) 2951
SmoI CTYRAG 1 cut(s) 2951
SpeI ACTAGT 1 cut(s) 2078
SspI AATATT 2 cut(s) 2440, 3520
SspMI CTAG 6 cut(s) 1502, 1755, 2079, 2693, 3032, 3125
StyD4I CCNGG 7 cut(s) 1470, 1809, 2218, 2219, 2932, 3446, 3534
TaiI ACGT 4 cut(s) 410, 3166, 3190, 3240
TaqI TCGA 9 cut(s) 12, 369, 382, 421, 616, 636, 1687, 3480, 3492
TatI WGTACW 4 cut(s) 1740, 2406, 2415, 3611
TauI GCSGC 3 cut(s) 266, 936, 1112
TscAI CASTG 8 cut(s) 913, 1737, 1918, 1929, 2862, 3174, 3287, 3318
TseFI GTSAC 4 cut(s) 43, 2356, 2671, 2720
TseI GCWGC 9 cut(s) 704, 968, 1112, 1117, 1490, 1908, 2706, 3214, 3555
Tsp45I GTSAC 4 cut(s) 43, 2356, 2671, 2720
TspGWI ACGGA 4 cut(s) 1270, 2077, 2332, 2604
TspMI CCCGGG 1 cut(s) 2219
TspRI CASTG 8 cut(s) 913, 1737, 1918, 1929, 2862, 3174, 3287, 3318
VpaK11BI GGWCC 5 cut(s) 341, 1332, 1468, 2852, 3152
XapI RAATTY 9 cut(s) 576, 1044, 1200, 1938, 2153, 2196, 2467, 2866, 3112
XbaI TCTAGA 1 cut(s) 3124
XceI RCATGY 2 cut(s) 965, 1904
XmaI CCCGGG 1 cut(s) 2219
XmiI GTMKAC 3 cut(s) 369, 921, 1423
XspI CTAG 6 cut(s) 1502, 1755, 2079, 2693, 3032, 3125
ZrmI AGTACT 1 cut(s) 1742
Zsp2I ATGCAT 2 cut(s) 1546, 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.