FvH4_3g45700

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37900551 .. 37905904
5354 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45700.t2

Sequence Viewer

Length: 3330 bp
ATGGTCTCCTCCTCTTCATCATCGTCAACTCGCAGCTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAAGGGATTGCAAACCAGATCTACAATCGACTTAGTAGAAGTGGCATTAAAACGTTCATGGATGAACCAGATCTTCGAGTAGGGGATACTATTTCGCCCACCCTCATGAAGGCAATTAAAGAGTCGAGGTTTGCAATCGTAATTCTCTCGCAAAATTACGCTTCTTCTGCTTGGTGTTTAGAGGAACTTCGAGAGATTTGTCTATCCAGGGAAGAGAACAGAATTTTGCCACTTTTTTTTGAAGTTGATCCTACTCATGTTCGATATCAGAAGAGGAGTTTCGAAGAAGCTTTCTCTAAGCACGATACCTCTCAGCGACATGAATTAGAGAAGGTGAATCAATGGAGGGCTGCCTTAAGCAAAGTGGCCAATATCTCAGGGTGGAACACAAATGATTTCAAGACTCACAATGAACTTGTTGACAAGATTGTGGATTCTCTTCGTAGTAAAGTACCACCTGATGCAATTGAATTCACAGATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAGACAAGGTTATGAAGGCGTTAACAGATGACAAGATTACTGCCGTTGGTGTCTATGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTACTTTTAACCATTGGATTATGGCTGTCGTATCCCAAGACCTTGACTTGAAAAAAATTCAAGGTACATTTTCAGATAAGTTGCGCTTCAAATTTGAGGAGGAGACAGAAGCTGGAAGAGCTGATAGGTTACATAAGGAGATAATGGGAAAAGAAAAGCTCCTCATAATCTTGGACGATGTATGGGCTGCAATAAAGTTATCAGAGATAGGGATTCCCACCTACAATGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCACCAGGAGATTGAATGTCTGTAATGCCATGAAGTGCCATGAAAAGATCAGCCTCAAAGTTCTCTCAGATCAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTTGAGCGCACCTCATTTGAGAAAGTGGCAAGGAGGGTAGCTGGAGAGTGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGTGGACCAGGAGGAATGGGAGAAGGCAGATGAACGACTAAAGCAGTCGCAAAAAGTCAATCCTAACCATGAGGAAGATGCATTCCGATGTATAAGATTAAGCTATAATTACTTGAAAGATGGGGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCGTAACATCAAAATAGAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCCCAAACAATGCAAGCAGCCAGAGGAAAATTAGTTACAGTGGTCAAATACCTGAAAAATTCTAGCTTGCTTTTGGATAGTGAGGAAGAAGGATGTGTTAAGATGCATGATGTCATCCGGGATACAGCCCTGAATATTGCACAATCTGAAGATGGGCATCGGTTTTTTGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGCCGCGAGGCGGATTACATGAAGGCCGCACTGCAATTTCACTGATGTGGAACCAAATTCGCAAGCTACCTGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAACGGGAATGCTAAACTAAGTGAGATCCCTGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCATATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAGACGAATGCGATGAATTGATTGACATTTCCATGGTGGGGAAACTTAAGAAGCTTGAAATTCTTAGTATGAGAGGCTGTCCCGGGGGGAAACTGTCTAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGACGTCAATCGCTATTGGGCAAGGAGAGGAGGATTGGTTACAATTCCATCTAAAGTGATATCAAAGTTGCAAAAATTAGAAGAATTTTACATGGTGTACTGTGGATTTGAGGAGGACGGGTTGTGTGTAGCTGAGGGACCAAGAGAAAGAACCAATATTATTAGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCTTATCCGATGAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATAACTATCAATGGCAGATGCACCAGCACTCGATCTGATGAATACAAACAAGGAGATCGTAATTCAAGATCCTTGTTTCTTAATGGAGTAACCATCAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATGAACGTTGCAGAGGGATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTTGCTGGGCATTGTTTTGATTCTTATGTGTACTTGAAAGAGTTGATGAACATAACAAGACGAGTTGAAACAGGACCAGTGTTTGAGAATTTGGAAGAGCTGCATCTGATGGATCTGATCCACCTGAAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCACAAATCTCAAGGTCTTTCATGTGTATTGTGGTTGGATTTTGAGGAGTGTATCAGAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGATTTGAACAATATGGATGAATTGGAATATGTGTTTAGATGCGAAGGGTTTGAGCCAGAGCAATCAAAACTGAGGGAGATGCATTTGCTGCGTCTAAAATCACTAAAAAGCATATGTAATGGTCCCGCTCCACATGCAATGTTCCAGAGTCTTAAGATTTTGACCATTGACCGTTGCATATTGCTGCAAAGTCTGTTCGCATCTGATGTAGCCCAATGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGTGGTGTCCTACGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAATTTGGTTTTGAAGAATCTTCCTAAGTTGTATGGTGCAATTGCTGCCGTTGATATTGAGTGTCCTTCATTGGAACGCTTGATGGTGGTGGATTGCCCCAAGCTTTTATTTTCAACCTCCTCCGATCTCTTCGAAGCCTTCAAAAGCAGGAACCAGTTTTCATTTTCAGCCTCCACTTCTGACCCGGTCCAACTCGATGATCCACAACTATACCTAGCTCTACGTGGCAGATCTTACCTATTCTGA

Protein Analysis

1110

Amino Acids

126.36

Weight (kDa)

6.9

Isoelectric Point (pI)

41.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 4 - 158 7.8e-29 NB-ARC domain
LRR_8 PF13855 367 - 425 4.6e-10 Leucine rich repeat
LRR_14 PF23598 372 - 503 1.5e-16 Leucine-rich repeat region
LRR_RPS2 PF23247 666 - 805 4.8e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 763 - 861 1.2e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2010
Acc36I ACCTGC 1 cut(s) 2200
AccBSI CCGCTC 1 cut(s) 2894
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 2 cut(s) 1394, 1621
AciI CCGC 6 cut(s) 660, 1619, 1626, 1642, 2405, 2892
AclI AACGTT 2 cut(s) 134, 2440
AclWI GGATC 8 cut(s) 323, 1747, 1762, 1806, 2343, 2623, 2631, 3278
AcoI YGGCCR 2 cut(s) 447, 1616
AcuI CTGAAG 4 cut(s) 1587, 1707, 2657, 3101
AcyI GRCGYC 1 cut(s) 2007
AfaI GTAC 8 cut(s) 534, 718, 781, 1391, 2102, 2380, 2543, 2739
AfiI CCNNNNNNNGG 5 cut(s) 190, 1625, 1908, 1995, 2638
AflII CTTAAG 3 cut(s) 436, 1916, 2918
AflIII ACRYGT 1 cut(s) 688
AhlI ACTAGT 1 cut(s) 1811
AjnI CCWGG 4 cut(s) 287, 980, 1197, 2665
AjuI GAANNNNNNNTTGG 4 cut(s) 1313, 1345, 2973, 3005
AleI CACNNNNGTG 1 cut(s) 1660
Alw26I GTCTC 6 cut(s) 10, 679, 812, 1847, 2676, 2737
AlwI GGATC 8 cut(s) 323, 1747, 1762, 1806, 2343, 2623, 2631, 3278
AlwNI CAGNNNCTG 3 cut(s) 827, 1777, 2516
Ama87I CYCGRG 1 cut(s) 1952
AoxI GGCC 3 cut(s) 447, 1616, 1639
ApeKI GCWGC 9 cut(s) 33, 431, 695, 902, 1436, 2611, 2854, 2950, 3128
Asp700I GAANNNNTTC 2 cut(s) 1087, 3003
AspLEI GCGC 2 cut(s) 801, 1101
AspS9I GGNCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
AsuC2I CCSGG 4 cut(s) 1538, 1953, 1954, 3269
AsuHPI GGTGA 6 cut(s) 427, 694, 967, 1840, 2419, 2666
AsuII TTCGAA 2 cut(s) 363, 3216
AvaI CYCGRG 1 cut(s) 1952
AvaII GGWCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
BaeGI GKGCMC 1 cut(s) 1183
BalI TGGCCA 1 cut(s) 449
BbsI GAAGAC 4 cut(s) 1362, 1383, 3006, 3068
BbvCI CCTCAGC 1 cut(s) 2136
BbvI GCAGC 9 cut(s) 45, 418, 682, 889, 1448, 2598, 2841, 2937, 3115
BccI CCATC 6 cut(s) 1304, 1565, 2059, 2381, 2614, 3160
BceAI ACGGC 2 cut(s) 623, 3116
BciT130I CCWGG 4 cut(s) 289, 982, 1199, 2667
BciVI GTATCC 3 cut(s) 160, 757, 1534
BcnI CCSGG 4 cut(s) 1538, 1953, 1954, 3269
BcoDI GTCTC 6 cut(s) 10, 679, 812, 1847, 2676, 2737
BcuI ACTAGT 1 cut(s) 1811
BfaI CTAG 6 cut(s) 1482, 1812, 1968, 2429, 2765, 3299
BfmI CTRYAG 1 cut(s) 706
BfrI CTTAAG 3 cut(s) 436, 1916, 2918
BfuAI ACCTGC 1 cut(s) 2200
BfuI GTATCC 3 cut(s) 160, 757, 1534
BglII AGATCT 3 cut(s) 99, 151, 3314
Bme1390I CCNGG 8 cut(s) 289, 982, 1199, 1538, 1953, 1954, 2667, 3269
Bme18I GGWCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
BmeT110I CYCGRG 1 cut(s) 1952
BmgT120I GGNCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
BmiI GGNNCC 5 cut(s) 69, 1667, 2142, 2890, 3236
BmrFI CCNGG 8 cut(s) 289, 982, 1199, 1538, 1953, 1954, 2667, 3269
BpiI GAAGAC 4 cut(s) 1362, 1383, 3006, 3068
BplI GAGNNNNNCTC 2 cut(s) 1088, 1120
BpmI CTGGAG 3 cut(s) 1155, 1697, 2777
Bpu10I CCTNAGC 1 cut(s) 2136
Bpu14I TTCGAA 2 cut(s) 363, 3216
BpuEI CTTGAG 2 cut(s) 2483, 2669
BpuMI CCSGG 4 cut(s) 1538, 1953, 1954, 3269
BsaAI YACGTR 1 cut(s) 3308
BsaBI GATNNNNATC 1 cut(s) 1575
BsaHI GRCGYC 1 cut(s) 2007
BsaI GGTCTC 2 cut(s) 10, 2676
BsaJI CCNNGG 6 cut(s) 288, 1902, 1952, 1953, 2479, 2666
BsaWI WCCGGW 1 cut(s) 1386
BsaXI ACNNNNNCTCC 4 cut(s) 2025, 2055, 2108, 2138
Bsc4I CCNNNNNNNGG 5 cut(s) 190, 1625, 1908, 1995, 2638
Bse1I ACTGG 2 cut(s) 2588, 3238
Bse3DI GCAATG 1 cut(s) 2910
Bse8I GATNNNNATC 1 cut(s) 1575
BseBI CCWGG 4 cut(s) 289, 982, 1199, 2667
BseDI CCNNGG 6 cut(s) 288, 1902, 1952, 1953, 2479, 2666
BseGI GGATG 6 cut(s) 148, 1517, 1533, 2235, 2457, 2788
BseJI GATNNNNATC 1 cut(s) 1575
BseLI CCNNNNNNNGG 5 cut(s) 190, 1625, 1908, 1995, 2638
BseMI GCAATG 1 cut(s) 2910
BseMII CTCAG 5 cut(s) 407, 471, 1056, 2127, 2828
BseNI ACTGG 2 cut(s) 2588, 3238
BseRI GAGGAG 9 cut(s) 370, 827, 830, 866, 1835, 2046, 2129, 2734, 3193
BseSI GKGCMC 1 cut(s) 1183
BseXI GCAGC 9 cut(s) 45, 418, 682, 889, 1448, 2598, 2841, 2937, 3115
BseYI CCCAGC 1 cut(s) 2516
BsgI GTGCAG 1 cut(s) 681
Bsh1236I CGCG 2 cut(s) 1394, 1621
Bsh1285I CGRYCG 1 cut(s) 1398
BshFI GGCC 3 cut(s) 449, 1618, 1641
BsiEI CGRYCG 1 cut(s) 1398
BsiHKCI CYCGRG 1 cut(s) 1952
BsiSI CCGG 4 cut(s) 1387, 1537, 1953, 3269
BslFI GGGAC 3 cut(s) 1935, 2154, 2874
BslI CCNNNNNNNGG 5 cut(s) 190, 1625, 1908, 1995, 2638
BsmAI GTCTC 6 cut(s) 10, 679, 812, 1847, 2676, 2737
BsmFI GGGAC 3 cut(s) 1935, 2154, 2874
BsmI GAATGC 3 cut(s) 1271, 1741, 1883
BsnI GGCC 3 cut(s) 449, 1618, 1641
Bso31I GGTCTC 2 cut(s) 10, 2676
BsoBI CYCGRG 1 cut(s) 1952
Bsp119I TTCGAA 2 cut(s) 363, 3216
Bsp1286I GDGCHC 1 cut(s) 1183
Bsp1407I TGTACA 1 cut(s) 2737
Bsp19I CCATGG 2 cut(s) 1902, 2479
BspACI CCGC 6 cut(s) 660, 1619, 1626, 1642, 2405, 2892
BspANI GGCC 3 cut(s) 449, 1618, 1641
BspCNI CTCAG 5 cut(s) 406, 470, 1055, 2128, 2829
BspFNI CGCG 2 cut(s) 1394, 1621
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 5 cut(s) 69, 1667, 2142, 2890, 3236
BspMAI CTGCAG 1 cut(s) 710
BspMI ACCTGC 1 cut(s) 2200
BspPI GGATC 8 cut(s) 323, 1747, 1762, 1806, 2343, 2623, 2631, 3278
BspQI GCTCTTC 2 cut(s) 826, 2601
BspT104I TTCGAA 2 cut(s) 363, 3216
BspTI CTTAAG 3 cut(s) 436, 1916, 2918
BspTNI GGTCTC 2 cut(s) 10, 2676
BsrBI CCGCTC 1 cut(s) 2894
BsrDI GCAATG 1 cut(s) 2910
BsrGI TGTACA 1 cut(s) 2737
BsrI ACTGG 2 cut(s) 2588, 3238
BssECI CCNNGG 6 cut(s) 288, 1902, 1952, 1953, 2479, 2666
BssNI GRCGYC 1 cut(s) 2007
BssT1I CCWWGG 2 cut(s) 1902, 2479
Bst2UI CCWGG 4 cut(s) 289, 982, 1199, 2667
Bst4CI ACNGT 7 cut(s) 1361, 1459, 1965, 2105, 2512, 2939, 3067
Bst6I CTCTTC 8 cut(s) 19, 288, 347, 525, 826, 1686, 2601, 3218
BstACI GRCGYC 1 cut(s) 2007
BstAFI CTTAAG 3 cut(s) 436, 1916, 2918
BstAPI GCANNNNNTGC 2 cut(s) 2854, 3128
BstAUI TGTACA 1 cut(s) 2737
BstBAI YACGTR 1 cut(s) 3308
BstBI TTCGAA 2 cut(s) 363, 3216
BstC8I GCNNGC 5 cut(s) 706, 1434, 1487, 1597, 1679
BstDSI CCRYGG 2 cut(s) 1902, 2479
BstENI CCTNNNNNAGG 1 cut(s) 188
BstF5I GGATG 6 cut(s) 148, 1517, 1533, 2235, 2457, 2788
BstFNI CGCG 2 cut(s) 1394, 1621
BstHHI GCGC 2 cut(s) 801, 1101
BstMAI GTCTC 6 cut(s) 10, 679, 812, 1847, 2676, 2737
BstMCI CGRYCG 1 cut(s) 1398
BstMWI GCNNNNNNNGC 9 cut(s) 248, 578, 660, 701, 833, 1166, 2854, 2900, 3128
BstNI CCWGG 4 cut(s) 289, 982, 1199, 2667
BstNSI RCATGY 2 cut(s) 692, 2903
BstSCI CCNGG 8 cut(s) 287, 980, 1197, 1536, 1951, 1952, 2665, 3267
BstSFI CTRYAG 1 cut(s) 706
BstSLI GKGCMC 1 cut(s) 1183
BstUI CGCG 2 cut(s) 1394, 1621
BstV1I GCAGC 9 cut(s) 45, 418, 682, 889, 1448, 2598, 2841, 2937, 3115
BstV2I GAAGAC 4 cut(s) 1362, 1383, 3006, 3068
BstX2I RGATCY 7 cut(s) 99, 151, 1752, 1798, 2348, 2623, 3314
BstXI CCANNNNNNTGG 1 cut(s) 575
BstYI RGATCY 7 cut(s) 99, 151, 1752, 1798, 2348, 2623, 3314
BsuI GTATCC 3 cut(s) 160, 757, 1534
BsuRI GGCC 3 cut(s) 449, 1618, 1641
BtgI CCRYGG 2 cut(s) 1902, 2479
BtgZI GCGATG 1 cut(s) 1896
BtsCI GGATG 6 cut(s) 148, 1517, 1533, 2235, 2457, 2788
BtsI GCAGTG 2 cut(s) 1644, 3054
BtsIMutI CAGTG 5 cut(s) 1464, 1644, 1655, 2595, 3054
BveI ACCTGC 1 cut(s) 2200
Cac8I GCNNGC 5 cut(s) 706, 1434, 1487, 1597, 1679
CaiI CAGNNNCTG 3 cut(s) 827, 1777, 2516
CciI TCATGA 1 cut(s) 186
CfoI GCGC 2 cut(s) 801, 1101
Cfr13I GGNCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
Cfr9I CCCGGG 1 cut(s) 1952
CseI GACGC 1 cut(s) 2846
Csp6I GTAC 8 cut(s) 533, 717, 780, 1390, 2101, 2379, 2542, 2738
CviQI GTAC 8 cut(s) 533, 717, 780, 1390, 2101, 2379, 2542, 2738
DraI TTTAAA 1 cut(s) 1608
EaeI YGGCCR 2 cut(s) 447, 1616
Eam1104I CTCTTC 8 cut(s) 19, 288, 347, 525, 826, 1686, 2601, 3218
EarI CTCTTC 8 cut(s) 19, 288, 347, 525, 826, 1686, 2601, 3218
EciI GGCGGA 1 cut(s) 1641
Eco130I CCWWGG 2 cut(s) 1902, 2479
Eco31I GGTCTC 2 cut(s) 10, 2676
Eco32I GATATC 2 cut(s) 347, 2064
Eco47I GGWCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
Eco57I CTGAAG 4 cut(s) 1587, 1707, 2657, 3101
Eco88I CYCGRG 1 cut(s) 1952
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 551
EcoRII CCWGG 4 cut(s) 287, 980, 1197, 2665
EcoRV GATATC 2 cut(s) 347, 2064
EcoT14I CCWWGG 2 cut(s) 1902, 2479
EcoT22I ATGCAT 3 cut(s) 1273, 1527, 2850
ErhI CCWWGG 2 cut(s) 1902, 2479
FalI AAGNNNNNCTT 6 cut(s) 785, 817, 1313, 1345, 2991, 3023
FaqI GGGAC 3 cut(s) 1935, 2154, 2874
FauI CCCGC 1 cut(s) 2899
FauNDI CATATG 1 cut(s) 2879
FblI GTMKAC 1 cut(s) 1150
FokI GGATG 6 cut(s) 155, 1520, 1524, 2222, 2464, 2795
FspBI CTAG 6 cut(s) 1482, 1812, 1968, 2429, 2765, 3299
GlaI GCGC 2 cut(s) 800, 1100
GsaI CCCAGC 1 cut(s) 2520
GsuI CTGGAG 3 cut(s) 1155, 1697, 2777
HaeIII GGCC 3 cut(s) 449, 1618, 1641
HapII CCGG 4 cut(s) 1387, 1537, 1953, 3269
HgaI GACGC 1 cut(s) 2846
HhaI GCGC 2 cut(s) 801, 1101
Hin1I GRCGYC 1 cut(s) 2007
Hin6I GCGC 2 cut(s) 799, 1099
HinP1I GCGC 2 cut(s) 799, 1099
HincII GTYRAC 3 cut(s) 27, 502, 618
HindII GTYRAC 3 cut(s) 27, 502, 618
HindIII AAGCTT 4 cut(s) 369, 1860, 1922, 3185
HpaI GTTAAC 1 cut(s) 618
HpaII CCGG 4 cut(s) 1387, 1537, 1953, 3269
HphI GGTGA 6 cut(s) 427, 694, 967, 1840, 2419, 2666
Hpy166II GTNNAC 8 cut(s) 27, 502, 618, 1151, 1195, 2101, 2542, 3052
Hpy8I GTNNAC 8 cut(s) 27, 502, 618, 1151, 1195, 2101, 2542, 3052
HpyCH4III ACNGT 7 cut(s) 1361, 1459, 1965, 2105, 2512, 2939, 3067
HpyCH4IV ACGT 5 cut(s) 134, 2007, 2440, 3026, 3307
HpyF10VI GCNNNNNNNGC 9 cut(s) 248, 578, 660, 701, 833, 1166, 2854, 2900, 3128
HpySE526I ACGT 5 cut(s) 134, 2007, 2440, 3026, 3307
Hsp92I GRCGYC 1 cut(s) 2007
HspAI GCGC 2 cut(s) 799, 1099
KspAI GTTAAC 1 cut(s) 618
LguI GCTCTTC 2 cut(s) 826, 2601
LmnI GCTCC 3 cut(s) 578, 879, 2899
Lsp1109I GCAGC 9 cut(s) 45, 418, 682, 889, 1448, 2598, 2841, 2937, 3115
MaeI CTAG 6 cut(s) 1482, 1812, 1968, 2429, 2765, 3299
MaeII ACGT 5 cut(s) 134, 2007, 2440, 3026, 3307
MaeIII GTNAC 9 cut(s) 843, 1361, 1453, 2041, 2368, 2407, 2456, 2487, 2658
MbiI CCGCTC 1 cut(s) 2894
MfeI CAATTG 3 cut(s) 546, 2993, 3123
MflI RGATCY 7 cut(s) 99, 151, 1752, 1798, 2348, 2623, 3314
MhlI GDGCHC 1 cut(s) 1183
MlsI TGGCCA 1 cut(s) 449
MluNI TGGCCA 1 cut(s) 449
MlyI GAGTC 3 cut(s) 212, 478, 2923
MmeI TCCRAC 4 cut(s) 646, 2690, 3009, 3298
Mox20I TGGCCA 1 cut(s) 449
Mph1103I ATGCAT 3 cut(s) 1273, 1527, 2850
MroXI GAANNNNTTC 2 cut(s) 1087, 3003
MscI TGGCCA 1 cut(s) 449
MslI CAYNNNNRTG 5 cut(s) 185, 1276, 1660, 1901, 2465
Msp20I TGGCCA 1 cut(s) 449
MspA1I CMGCKG 1 cut(s) 36
MspCI CTTAAG 3 cut(s) 436, 1916, 2918
MspI CCGG 4 cut(s) 1387, 1537, 1953, 3269
MspR9I CCNGG 8 cut(s) 289, 982, 1199, 1538, 1953, 1954, 2667, 3269
MunI CAATTG 3 cut(s) 546, 2993, 3123
Mva1269I GAATGC 3 cut(s) 1271, 1741, 1883
MvaI CCWGG 4 cut(s) 289, 982, 1199, 2667
MvnI CGCG 2 cut(s) 1394, 1621
MwoI GCNNNNNNNGC 9 cut(s) 248, 578, 660, 701, 833, 1166, 2854, 2900, 3128
NciI CCSGG 4 cut(s) 1538, 1953, 1954, 3269
NcoI CCATGG 2 cut(s) 1902, 2479
NdeI CATATG 1 cut(s) 2879
NlaIV GGNNCC 5 cut(s) 69, 1667, 2142, 2890, 3236
NmuCI GTSAC 2 cut(s) 2407, 2456
NsiI ATGCAT 3 cut(s) 1273, 1527, 2850
NspI RCATGY 2 cut(s) 692, 2903
NspV TTCGAA 2 cut(s) 363, 3216
OliI CACNNNNGTG 1 cut(s) 1660
PagI TCATGA 1 cut(s) 186
PciI ACATGT 1 cut(s) 688
PciSI GCTCTTC 2 cut(s) 826, 2601
PctI GAATGC 3 cut(s) 1271, 1741, 1883
PdmI GAANNNNTTC 2 cut(s) 1087, 3003
PfeI GAWTC 8 cut(s) 418, 515, 928, 1052, 1316, 1774, 2531, 3100
PflFI GACNNNGTC 1 cut(s) 3269
PfoI TCCNGGA 1 cut(s) 1536
Ple19I CGATCG 1 cut(s) 1398
PleI GAGTC 3 cut(s) 211, 478, 2922
PpsI GAGTC 3 cut(s) 211, 478, 2922
Ppu21I YACGTR 1 cut(s) 3308
PpuMI RGGWCCY 1 cut(s) 68
PscI ACATGT 1 cut(s) 688
Psp1406I AACGTT 2 cut(s) 134, 2440
Psp5II RGGWCCY 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 287, 980, 1197, 2665
PspFI CCCAGC 1 cut(s) 2516
PspGI CCWGG 4 cut(s) 287, 980, 1197, 2665
PspN4I GGNNCC 5 cut(s) 69, 1667, 2142, 2890, 3236
PspPI GGNCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
PspPPI RGGWCCY 1 cut(s) 68
PstI CTGCAG 1 cut(s) 710
PstNI CAGNNNCTG 3 cut(s) 827, 1777, 2516
PsuI RGATCY 7 cut(s) 99, 151, 1752, 1798, 2348, 2623, 3314
PsyI GACNNNGTC 1 cut(s) 3269
PvuI CGATCG 1 cut(s) 1398
PvuII CAGCTG 1 cut(s) 36
RsaI GTAC 8 cut(s) 534, 718, 781, 1391, 2102, 2380, 2543, 2739
RsaNI GTAC 8 cut(s) 533, 717, 780, 1390, 2101, 2379, 2542, 2738
RseI CAYNNNNRTG 5 cut(s) 185, 1276, 1660, 1901, 2465
SapI GCTCTTC 2 cut(s) 826, 2601
Sau96I GGNCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
SchI GAGTC 3 cut(s) 212, 478, 2923
ScrFI CCNGG 8 cut(s) 289, 982, 1199, 1538, 1953, 1954, 2667, 3269
SduI GDGCHC 1 cut(s) 1183
SfcI CTRYAG 1 cut(s) 706
SfuI TTCGAA 2 cut(s) 363, 3216
SinI GGWCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
SmaI CCCGGG 1 cut(s) 1954
SmiMI CAYNNNNRTG 5 cut(s) 185, 1276, 1660, 1901, 2465
SmlI CTYRAG 5 cut(s) 436, 1916, 2498, 2684, 2918
SmoI CTYRAG 5 cut(s) 436, 1916, 2498, 2684, 2918
SpeI ACTAGT 1 cut(s) 1811
SsiI CCGC 6 cut(s) 660, 1619, 1626, 1642, 2405, 2892
SspI AATATT 3 cut(s) 1555, 2161, 2205
SspMI CTAG 6 cut(s) 1482, 1812, 1968, 2429, 2765, 3299
StyD4I CCNGG 8 cut(s) 287, 980, 1197, 1536, 1951, 1952, 2665, 3267
StyI CCWWGG 2 cut(s) 1902, 2479
TaaI ACNGT 7 cut(s) 1361, 1459, 1965, 2105, 2512, 2939, 3067
TaiI ACGT 5 cut(s) 137, 2010, 2443, 3029, 3310
TatI WGTACW 3 cut(s) 2100, 2541, 2737
TauI GCSGC 3 cut(s) 663, 1621, 1644
TfiI GAWTC 8 cut(s) 418, 515, 928, 1052, 1316, 1774, 2531, 3100
TscAI CASTG 5 cut(s) 1464, 1651, 1662, 2595, 3054
TseFI GTSAC 2 cut(s) 2407, 2456
TseI GCWGC 9 cut(s) 33, 431, 695, 902, 1436, 2611, 2854, 2950, 3128
Tsp45I GTSAC 2 cut(s) 2407, 2456
TspMI CCCGGG 1 cut(s) 1952
TspRI CASTG 5 cut(s) 1464, 1651, 1662, 2595, 3054
Tth111I GACNNNGTC 1 cut(s) 3269
Vha464I CTTAAG 3 cut(s) 436, 1916, 2918
VpaK11BI GGWCC 7 cut(s) 68, 1059, 1195, 2141, 2585, 2888, 3271
XagI CCTNNNNNAGG 1 cut(s) 188
XbaI TCTAGA 1 cut(s) 1967
XceI RCATGY 2 cut(s) 692, 2903
XmaI CCCGGG 1 cut(s) 1952
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 2 cut(s) 1087, 3003
XspI CTAG 6 cut(s) 1482, 1812, 1968, 2429, 2765, 3299
ZraI GACGTC 1 cut(s) 2008
Zsp2I ATGCAT 3 cut(s) 1273, 1527, 2850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.