RchiOBHm_Chr5g0076771

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Forward (+)
82513005 .. 82519144
6140 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35140

Sequence Viewer

Length: 2991 bp
ATGGAGTTCTTCGAGATATCGGTGGCGATTATCGTGGTAATTGTGACATGGAGTTGCAAGGCCATTATTAAGTGGATATGGACTCTAGTGAAGCGTTGGTTCAGTTATATGGTCGGATGGTCGGAGGATAATCAAATATTGGTTGACATGGATGCCTCTGCATCTCTTTCTTCTTCATCAACGGATCGTCGGTGTAAGCATCATGTGTTTTTGAGTTTCAGGGGTCCTGACGCTCGCAAGGGTATCGCATTTGAAGTATACAATCGACTGCAGGGGAGGGGAATTAAAACATTCAAGGATGATCGAGATCTTGAAGTAGGGGATGCTATTTCTTCCACTCTAATAGCAGCAATTGAAGAATCAAGGTTTGCAATTGTTGTTCTCTCGCGAGACTACGCTTCTTCTACTTGGTGTTTGGAGGAACTTGCAAAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTCTTCCACTTTTCTATAATGTGGAACCCTCTGATGTACGATTTCAGAAGAAGAGTTTCAAAGAAGCTTTTGATAAGCATGTAACCTCTGGGCGACACAGATCGGAGAAGGTGAAGCAATGGAGAGATGCTTTAAACAAAGTGGCCGGTTTCTCTGGCTGGAATATGCAGGATTATAATTCTGAGAGAGAACTTGTGGACGCAATTGTGTATTTTGTGTACAGTAAAATTGAGTCCACATTGTCCACCGGAGATTTTGAAGCATTTGAAGCAACAAAAAAAGCCATGGATGAGGTTATGGAGGGGCTACAAGATGACGAGGTCACCGCAACGGCGGTCTACGGAATGGGCGGCGTTGGCAAGACAACCATGGTGAAAGAAGTCGGTGCACAATTCAGAAAAAGTGGGGTGTTTCATCGTGTCATTATGGGTGTCGTTTCAAAAAATCCTGATTCCAAAAACCTTCAGGACACATTGGCAGAGCAGTTGGGCTTCAAATTGTGGCAGGAGACAGAAATTGGAAGAGCTGCTTGGTTGCATGAAGAGATAATGAGAAGAGAAAAACTCCTTATAATCTTGGACGATGTTTGGGAGAGAACAGACTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAAAGTGCAAATCCAAAGTCATACTCACCACAAGGATATCGAATGTCTGTCATACCATGGAGTGCCAAAGAAAGATCACCCTCAATATCTTATCAGAACAAGATTCTTGGAAATTGTTTATGTCAAAAGCAAGACAGTCTTTTGAATCGACCGAATTCAAAGATATAGCAAGGAAGGTAGCTGGAGAATGTAAGGGTCTTCCAGTTGCATTGATAGCAGTTGCAAGGGCACTAGGAGATAAAGACCTAGCAGAATGGGAAAAAGCAGCTCAACGGCTACAGAAGTCACAACGTGCCAACCCTGACGATAAGGGAGATGTGTTCGAATGTATAAAATTAAGCTATGATCACTTGAAAGATGAGGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGACCATGACATCCGACTAGGAAACTTATTCAAGTATGCAATCGGGACAGGATTGTTTCGACGAGATGCCGTTACAATCGAAGAAGCCAGAGGAATAGCAGATTCAGCGGTCAAGTACCTGAAAGCTTCAAGCTTGCTTTTGGATAGCGAGAAAAATGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCCTGAATATTGCAAAATCTAAAGATGGGCACGAGTTTCTGGTAAAAGCTGGCTATGGATTAAAGCATTGGCCATGTGGATCACATCAAGGCTACTCTGCAATCTCACTAATGAGGAACGAAATTCACAAGCTGCCCGAAGAAGAGTTGGTTGGAATATCTGATGCAAAATACATCCCTAAAACTGTTGAGGCCAAACCAGATTGGGATTACTTCCATATAAGTATCATCGGCAGACACGCCTCCATTAAACACACAGGAAGAAACCATAATTCAAGATCTTTGTTTCTTAATGGAGCAACCATGAGTACCTTGCCTGATTGGTTTATCAACGCGGTGACAAAGAAAACAGAGAAGCTAGAGTACATGAGCTGCAAAGGGATGAGTGACATTCTTATGGAACATGACCATGGAAGGTTACATAAACTCAAGCATCTCAAAGTATGGGATTATTTTCTTGATTCCTCTGTGTGCTTGAAAGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTGAGAAGTTGGAAGAGTTGCATCTGATACATCTGATCCACCTTGAGGAGTTGTGTGTTGGCGAGTTACCACCTGGGTCTCTCTCGAATCTGAAGGAATTGCATATTTTTTTTTGTAATATCTTGAAGAATGTATCAAAATTGGTACAGAGACTACCAAATCTGGAGAAACTAGATTTAAATTGTTTGTATAATTTGGAATATGTCTTTGTATCGGAAGGGTTTGAGCCGAAAGAATCAAAAATGAGAGAGATATATTTGTCGGGTCCATGTTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCGAGAGTCTTAAACTTTTGACCTTTTACCGTTGCGAGTTGCTGCAAAGTCTCTTCGCATCTGATGTAGCTAAGTGTCTTGATCAATTGGAAGACCTTTTTGTAGAGCAGTGCCCTTTGTTGGAAAGAGTAATTGAAGCAGCGAACAACAAGAAGACGGTTTTTCCAAAAATGAAGAACTTGGTTTTGAAGAATCTTCCTCTGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTCATTGGAACTCTTGACTGTGGTGGATTGCGCCCAGATTTCATTTTCAACCACTTCCTCTCTCTTCCACTCCTTCGAAAGCAGGAACCAGTTTTCATTTTCAACCTCTGCTTCTAACTACTTTGGCAGCACGACCCCAGTCCAACTCAATGATCCACAACTGTACCAATTTGTACATGACAGATGCAGGAGTATATCTGGTTTCCAATCGTGA

Protein Analysis

996

Amino Acids

113.43

Weight (kDa)

6.87

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 66 - 238 2.5e-46 TIR domain
TIR_2 PF13676 69 - 164 1.1e-15 TIR domain
NB-ARC PF00931 247 - 413 6.3e-31 NB-ARC domain
LRR_RPS2 PF23247 831 - 939 4.5e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 639, 1034
AccI GTMKAC 2 cut(s) 258, 801
AccII CGCG 3 cut(s) 388, 2033, 2529
AciI CCGC 5 cut(s) 789, 797, 813, 1613, 2033
AclWI GGATC 4 cut(s) 192, 1786, 2251, 2924
AcoI YGGCCR 2 cut(s) 606, 1769
AcsI RAATTY 3 cut(s) 462, 1256, 1821
AcuI CTGAAG 2 cut(s) 911, 2333
AdeI CACNNNGTG 1 cut(s) 1394
AfaI GTAC 8 cut(s) 501, 683, 1622, 2008, 2063, 2367, 2942, 2952
AfiI CCNNNNNNNGG 2 cut(s) 2266, 2656
AflIII ACRYGT 1 cut(s) 2527
AhdI GACNNNNNGTC 1 cut(s) 1471
AjnI CCWGG 1 cut(s) 2293
AjuI GAANNNNNNNTTGG 4 cut(s) 976, 1008, 1833, 1865
Alw21I GWGCWC 1 cut(s) 853
Alw26I GTCTC 5 cut(s) 384, 965, 2304, 2365, 2591
Alw44I GTGCAC 1 cut(s) 849
AlwI GGATC 4 cut(s) 192, 1786, 2251, 2924
AoxI GGCC 4 cut(s) 60, 606, 1769, 1890
ApaLI GTGCAC 1 cut(s) 849
ApeKI GCWGC 8 cut(s) 347, 989, 1367, 1831, 2070, 2578, 2675, 2904
ApoI RAATTY 3 cut(s) 462, 1256, 1821
ArsI GACNNNNNNTTYG 2 cut(s) 1104, 1136
Asp700I GAANNNNTTC 1 cut(s) 518
AspLEI GCGC 1 cut(s) 2810
AspS9I GGNCC 5 cut(s) 224, 1465, 2213, 2486, 2516
AsuC2I CCSGG 1 cut(s) 1691
AsuHPI GGTGA 6 cut(s) 586, 778, 847, 1120, 1171, 2047
AsuII TTCGAA 2 cut(s) 1425, 2853
AvaII GGWCC 5 cut(s) 224, 1465, 2213, 2486, 2516
BaeGI GKGCMC 4 cut(s) 853, 1333, 1731, 2651
BaeI ACNNNNGTAYC 2 cut(s) 2924, 2957
BalI TGGCCA 1 cut(s) 1771
BarI GAAGNNNNNNTAC 2 cut(s) 2045, 2077
BauI CACGAG 1 cut(s) 1730
BbsI GAAGAC 4 cut(s) 1292, 1512, 2634, 2696
Bbv12I GWGCWC 1 cut(s) 853
BbvI GCAGC 8 cut(s) 359, 976, 1379, 1818, 2057, 2565, 2687, 2916
BccI CCATC 2 cut(s) 111, 1718
BceAI ACGGC 3 cut(s) 810, 1391, 1559
BcgI CGANNNNNNTGC 2 cut(s) 226, 260
BciT130I CCWGG 1 cut(s) 2295
BciVI GTATCC 1 cut(s) 1687
BclI TGATCA 2 cut(s) 1447, 2617
BcnI CCSGG 1 cut(s) 1691
BcoDI GTCTC 5 cut(s) 384, 965, 2304, 2365, 2591
BfaI CTAG 6 cut(s) 86, 1334, 1349, 1523, 2057, 2393
BfmI CTRYAG 2 cut(s) 269, 1379
BfuI GTATCC 1 cut(s) 1687
BglII AGATCT 3 cut(s) 307, 432, 1976
BisI GCNGC 9 cut(s) 348, 814, 990, 1368, 1832, 2071, 2579, 2676, 2905
BlsI GCNGC 9 cut(s) 349, 815, 991, 1369, 1833, 2072, 2580, 2677, 2906
Bme1390I CCNGG 2 cut(s) 1691, 2295
Bme18I GGWCC 5 cut(s) 224, 1465, 2213, 2486, 2516
BmeRI GACNNNNNGTC 1 cut(s) 1471
BmgT120I GGNCC 5 cut(s) 224, 1465, 2213, 2486, 2516
BmiI GGNNCC 4 cut(s) 225, 489, 2487, 2864
BmrFI CCNGG 2 cut(s) 1691, 2295
BmrI ACTGGG 1 cut(s) 2909
BmuI ACTGGG 1 cut(s) 2909
BoxI GACNNNNGTC 1 cut(s) 2915
BpiI GAAGAC 4 cut(s) 1292, 1512, 2634, 2696
BplI GAGNNNNNCTC 2 cut(s) 1011, 1043
BpmI CTGGAG 2 cut(s) 1305, 2405
Bpu14I TTCGAA 2 cut(s) 1425, 2853
BpuEI CTTGAG 2 cut(s) 2111, 2285
BpuMI CCSGG 1 cut(s) 1691
BsaBI GATNNNNATC 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 2304
BsaJI CCNNGG 5 cut(s) 747, 831, 1158, 2107, 2294
BsaWI WCCGGW 1 cut(s) 710
Bsc4I CCNNNNNNNGG 2 cut(s) 2266, 2656
Bse118I RCCGGY 1 cut(s) 608
Bse1I ACTGG 4 cut(s) 1304, 2216, 2866, 2915
Bse3DI GCAATG 1 cut(s) 587
Bse8I GATNNNNATC 1 cut(s) 306
BseBI CCWGG 1 cut(s) 2295
BseDI CCNNGG 5 cut(s) 747, 831, 1158, 2107, 2294
BseJI GATNNNNATC 1 cut(s) 306
BseLI CCNNNNNNNGG 2 cut(s) 2266, 2656
BseMI GCAATG 1 cut(s) 587
BseMII CTCAG 1 cut(s) 636
BseNI ACTGG 4 cut(s) 1304, 2216, 2866, 2915
BseRI GAGGAG 1 cut(s) 2282
BseSI GKGCMC 4 cut(s) 853, 1333, 1731, 2651
BseXI GCAGC 8 cut(s) 359, 976, 1379, 1818, 2057, 2565, 2687, 2916
BseYI CCCAGC 1 cut(s) 1085
Bsh1236I CGCG 3 cut(s) 388, 2033, 2529
Bsh1285I CGRYCG 1 cut(s) 1254
BshFI GGCC 4 cut(s) 62, 608, 1771, 1892
BsiEI CGRYCG 1 cut(s) 1254
BsiHKAI GWGCWC 1 cut(s) 853
BsiSI CCGG 3 cut(s) 609, 711, 1690
BslFI GGGAC 1 cut(s) 1564
BslI CCNNNNNNNGG 2 cut(s) 2266, 2656
BsmAI GTCTC 5 cut(s) 384, 965, 2304, 2365, 2591
BsmFI GGGAC 1 cut(s) 1564
BsnI GGCC 4 cut(s) 62, 608, 1771, 1892
Bso31I GGTCTC 1 cut(s) 2304
Bsp119I TTCGAA 2 cut(s) 1425, 2853
Bsp1286I GDGCHC 4 cut(s) 853, 1333, 1731, 2651
Bsp1407I TGTACA 2 cut(s) 681, 2950
Bsp19I CCATGG 4 cut(s) 747, 831, 1158, 2107
Bsp68I TCGCGA 1 cut(s) 388
BspACI CCGC 5 cut(s) 789, 797, 813, 1613, 2033
BspANI GGCC 4 cut(s) 62, 608, 1771, 1892
BspCNI CTCAG 1 cut(s) 637
BspFNI CGCG 3 cut(s) 388, 2033, 2529
BspLI GGNNCC 4 cut(s) 225, 489, 2487, 2864
BspMAI CTGCAG 1 cut(s) 273
BspPI GGATC 4 cut(s) 192, 1786, 2251, 2924
BspQI GCTCTTC 2 cut(s) 979, 2495
BspT104I TTCGAA 2 cut(s) 1425, 2853
BspTNI GGTCTC 1 cut(s) 2304
BsrDI GCAATG 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 608
BsrGI TGTACA 2 cut(s) 681, 2950
BsrI ACTGG 4 cut(s) 1304, 2216, 2866, 2915
BssAI RCCGGY 1 cut(s) 608
BssECI CCNNGG 5 cut(s) 747, 831, 1158, 2107, 2294
BssNAI GTATAC 1 cut(s) 259
BssSI CACGAG 1 cut(s) 1730
BssT1I CCWWGG 4 cut(s) 747, 831, 1158, 2107
Bst1107I GTATAC 1 cut(s) 259
Bst2BI CACGAG 1 cut(s) 1730
Bst2UI CCWGG 1 cut(s) 2295
Bst4CI ACNGT 8 cut(s) 686, 1239, 1885, 2567, 2695, 2761, 2797, 2940
Bst6I CTCTTC 9 cut(s) 509, 979, 999, 1012, 1836, 2229, 2495, 2594, 2846
BstAUI TGTACA 2 cut(s) 681, 2950
BstBI TTCGAA 2 cut(s) 1425, 2853
BstC8I GCNNGC 3 cut(s) 235, 1640, 1750
BstDEI CTNAG 2 cut(s) 645, 2607
BstDSI CCRYGG 4 cut(s) 747, 831, 1158, 2107
BstEII GGTNACC 1 cut(s) 784
BstFNI CGCG 3 cut(s) 388, 2033, 2529
BstHHI GCGC 1 cut(s) 2810
BstMAI GTCTC 5 cut(s) 384, 965, 2304, 2365, 2591
BstMCI CGRYCG 1 cut(s) 1254
BstMWI GCNNNNNNNGC 4 cut(s) 731, 819, 1316, 1610
BstNI CCWGG 1 cut(s) 2295
BstNSI RCATGY 1 cut(s) 545
BstPAI GACNNNNGTC 1 cut(s) 2915
BstPI GGTNACC 1 cut(s) 784
BstSCI CCNGG 2 cut(s) 1689, 2293
BstSFI CTRYAG 2 cut(s) 269, 1379
BstSLI GKGCMC 4 cut(s) 853, 1333, 1731, 2651
BstUI CGCG 3 cut(s) 388, 2033, 2529
BstV1I GCAGC 8 cut(s) 359, 976, 1379, 1818, 2057, 2565, 2687, 2916
BstV2I GAAGAC 4 cut(s) 1292, 1512, 2634, 2696
BstX2I RGATCY 3 cut(s) 307, 432, 1976
BstYI RGATCY 3 cut(s) 307, 432, 1976
BstZ17I GTATAC 1 cut(s) 259
BsuI GTATCC 1 cut(s) 1687
BsuRI GGCC 4 cut(s) 62, 608, 1771, 1892
BtgI CCRYGG 4 cut(s) 747, 831, 1158, 2107
BtsI GCAGTG 1 cut(s) 2651
BtsIMutI CAGTG 3 cut(s) 2223, 2502, 2651
BtuMI TCGCGA 1 cut(s) 388
Cac8I GCNNGC 3 cut(s) 235, 1640, 1750
CfoI GCGC 1 cut(s) 2810
Cfr10I RCCGGY 1 cut(s) 608
Cfr13I GGNCC 5 cut(s) 224, 1465, 2213, 2486, 2516
CseI GACGC 2 cut(s) 239, 671
Csp6I GTAC 8 cut(s) 500, 682, 1621, 2007, 2062, 2366, 2941, 2951
CviQI GTAC 8 cut(s) 500, 682, 1621, 2007, 2062, 2366, 2941, 2951
DdeI CTNAG 2 cut(s) 645, 2607
DraI TTTAAA 2 cut(s) 597, 2400
DraIII CACNNNGTG 1 cut(s) 1394
DriI GACNNNNNGTC 1 cut(s) 1471
EaeI YGGCCR 2 cut(s) 606, 1769
Eam1104I CTCTTC 9 cut(s) 509, 979, 999, 1012, 1836, 2229, 2495, 2594, 2846
Eam1105I GACNNNNNGTC 1 cut(s) 1471
EarI CTCTTC 9 cut(s) 509, 979, 999, 1012, 1836, 2229, 2495, 2594, 2846
Eco130I CCWWGG 4 cut(s) 747, 831, 1158, 2107
Eco31I GGTCTC 1 cut(s) 2304
Eco32I GATATC 2 cut(s) 18, 1140
Eco47I GGWCC 5 cut(s) 224, 1465, 2213, 2486, 2516
Eco57I CTGAAG 2 cut(s) 911, 2333
Eco91I GGTNACC 1 cut(s) 784
EcoO109I RGGNCCY 1 cut(s) 224
EcoO65I GGTNACC 1 cut(s) 784
EcoRI GAATTC 2 cut(s) 462, 1256
EcoRII CCWGG 1 cut(s) 2293
EcoRV GATATC 2 cut(s) 18, 1140
EcoT14I CCWWGG 4 cut(s) 747, 831, 1158, 2107
EcoT22I ATGCAT 1 cut(s) 1680
ErhI CCWWGG 4 cut(s) 747, 831, 1158, 2107
FalI AAGNNNNNCTT 6 cut(s) 976, 1008, 1225, 1257, 1463, 1495
FaqI GGGAC 1 cut(s) 1564
FbaI TGATCA 2 cut(s) 1447, 2617
FblI GTMKAC 2 cut(s) 258, 801
Fnu4HI GCNGC 9 cut(s) 348, 814, 990, 1368, 1832, 2071, 2579, 2676, 2905
Fsp4HI GCNGC 9 cut(s) 348, 814, 990, 1368, 1832, 2071, 2579, 2676, 2905
FspBI CTAG 6 cut(s) 86, 1334, 1349, 1523, 2057, 2393
GlaI GCGC 1 cut(s) 2809
GluI GCNGC 9 cut(s) 348, 814, 990, 1368, 1832, 2071, 2579, 2676, 2905
GsaI CCCAGC 1 cut(s) 1089
GsuI CTGGAG 2 cut(s) 1305, 2405
HaeIII GGCC 4 cut(s) 62, 608, 1771, 1892
HapII CCGG 3 cut(s) 609, 711, 1690
HgaI GACGC 2 cut(s) 239, 671
HhaI GCGC 1 cut(s) 2810
Hin6I GCGC 1 cut(s) 2808
HinP1I GCGC 1 cut(s) 2808
HincII GTYRAC 1 cut(s) 145
HindII GTYRAC 1 cut(s) 145
HindIII AAGCTT 3 cut(s) 528, 1629, 1636
HpaII CCGG 3 cut(s) 609, 711, 1690
HphI GGTGA 6 cut(s) 586, 778, 847, 1120, 1171, 2047
Hpy166II GTNNAC 8 cut(s) 145, 259, 661, 682, 699, 708, 802, 851
Hpy8I GTNNAC 8 cut(s) 145, 259, 661, 682, 699, 708, 802, 851
Hpy99I CGWCG 2 cut(s) 192, 1569
HpyAV CCTTC 8 cut(s) 565, 935, 1270, 2106, 2308, 2432, 2787, 2860
HpyCH4III ACNGT 8 cut(s) 686, 1239, 1885, 2567, 2695, 2761, 2797, 2940
HpyCH4IV ACGT 1 cut(s) 1393
HpyF10VI GCNNNNNNNGC 4 cut(s) 731, 819, 1316, 1610
HpyF3I CTNAG 2 cut(s) 645, 2607
HpySE526I ACGT 1 cut(s) 1393
HspAI GCGC 1 cut(s) 2808
Ksp22I TGATCA 2 cut(s) 1447, 2617
LguI GCTCTTC 2 cut(s) 979, 2495
LmnI GCTCC 2 cut(s) 1096, 1994
Lsp1109I GCAGC 8 cut(s) 359, 976, 1379, 1818, 2057, 2565, 2687, 2916
MaeI CTAG 6 cut(s) 86, 1334, 1349, 1523, 2057, 2393
MaeII ACGT 1 cut(s) 1393
MaeIII GTNAC 9 cut(s) 43, 544, 784, 1386, 1576, 2035, 2084, 2115, 2286
MfeI CAATTG 4 cut(s) 351, 372, 666, 2621
MflI RGATCY 3 cut(s) 307, 432, 1976
MhlI GDGCHC 4 cut(s) 853, 1333, 1731, 2651
MlsI TGGCCA 1 cut(s) 1771
MluI ACGCGT 1 cut(s) 2527
MluNI TGGCCA 1 cut(s) 1771
MlyI GAGTC 3 cut(s) 76, 704, 2551
MmeI TCCRAC 7 cut(s) 94, 102, 1543, 1831, 2211, 2637, 2944
Mox20I TGGCCA 1 cut(s) 1771
Mph1103I ATGCAT 1 cut(s) 1680
MroXI GAANNNNTTC 1 cut(s) 518
MscI TGGCCA 1 cut(s) 1771
MseI TTAA 9 cut(s) 69, 285, 596, 1439, 1760, 1947, 1989, 2399, 2547
MslI CAYNNNNRTG 2 cut(s) 2093, 2106
Msp20I TGGCCA 1 cut(s) 1771
MspA1I CMGCKG 1 cut(s) 1613
MspI CCGG 3 cut(s) 609, 711, 1690
MspR9I CCNGG 2 cut(s) 1691, 2295
MunI CAATTG 4 cut(s) 351, 372, 666, 2621
MvaI CCWGG 1 cut(s) 2295
MvnI CGCG 3 cut(s) 388, 2033, 2529
MwoI GCNNNNNNNGC 4 cut(s) 731, 819, 1316, 1610
NciI CCSGG 1 cut(s) 1691
NcoI CCATGG 4 cut(s) 747, 831, 1158, 2107
NlaIV GGNNCC 4 cut(s) 225, 489, 2487, 2864
NmuCI GTSAC 5 cut(s) 43, 784, 1386, 2035, 2084
NruI TCGCGA 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 1680
NspI RCATGY 1 cut(s) 545
NspV TTCGAA 2 cut(s) 1425, 2853
PciSI GCTCTTC 2 cut(s) 979, 2495
PdmI GAANNNNTTC 1 cut(s) 518
PflFI GACNNNGTC 1 cut(s) 782
PfoI TCCNGGA 1 cut(s) 1689
PkrI GCNGC 9 cut(s) 349, 815, 991, 1369, 1833, 2072, 2580, 2677, 2906
PleI GAGTC 3 cut(s) 76, 703, 2550
PpsI GAGTC 3 cut(s) 76, 703, 2550
PpuMI RGGWCCY 1 cut(s) 224
PshAI GACNNNNGTC 1 cut(s) 2915
PsiI TTATAA 2 cut(s) 639, 1034
Psp5II RGGWCCY 1 cut(s) 224
Psp6I CCWGG 1 cut(s) 2293
PspEI GGTNACC 1 cut(s) 784
PspFI CCCAGC 1 cut(s) 1085
PspGI CCWGG 1 cut(s) 2293
PspN4I GGNNCC 4 cut(s) 225, 489, 2487, 2864
PspPI GGNCC 5 cut(s) 224, 1465, 2213, 2486, 2516
PspPPI RGGWCCY 1 cut(s) 224
PstI CTGCAG 1 cut(s) 273
PsuI RGATCY 3 cut(s) 307, 432, 1976
PsyI GACNNNGTC 1 cut(s) 782
RruI TCGCGA 1 cut(s) 388
RsaI GTAC 8 cut(s) 501, 683, 1622, 2008, 2063, 2367, 2942, 2952
RsaNI GTAC 8 cut(s) 500, 682, 1621, 2007, 2062, 2366, 2941, 2951
RseI CAYNNNNRTG 2 cut(s) 2093, 2106
SapI GCTCTTC 2 cut(s) 979, 2495
SaqAI TTAA 9 cut(s) 69, 285, 596, 1439, 1760, 1947, 1989, 2399, 2547
SatI GCNGC 9 cut(s) 348, 814, 990, 1368, 1832, 2071, 2579, 2676, 2905
Sau96I GGNCC 5 cut(s) 224, 1465, 2213, 2486, 2516
SchI GAGTC 3 cut(s) 76, 704, 2551
ScrFI CCNGG 2 cut(s) 1691, 2295
SduI GDGCHC 4 cut(s) 853, 1333, 1731, 2651
SfcI CTRYAG 2 cut(s) 269, 1379
SfuI TTCGAA 2 cut(s) 1425, 2853
SinI GGWCC 5 cut(s) 224, 1465, 2213, 2486, 2516
SmiI ATTTAAAT 1 cut(s) 2400
SmiMI CAYNNNNRTG 2 cut(s) 2093, 2106
SmlI CTYRAG 2 cut(s) 2126, 2264
SmoI CTYRAG 2 cut(s) 2126, 2264
SsiI CCGC 5 cut(s) 789, 797, 813, 1613, 2033
SspI AATATT 2 cut(s) 138, 1708
SspMI CTAG 6 cut(s) 86, 1334, 1349, 1523, 2057, 2393
StyD4I CCNGG 2 cut(s) 1689, 2293
StyI CCWWGG 4 cut(s) 747, 831, 1158, 2107
SwaI ATTTAAAT 1 cut(s) 2400
TaaI ACNGT 8 cut(s) 686, 1239, 1885, 2567, 2695, 2761, 2797, 2940
TaiI ACGT 1 cut(s) 1396
TaqII GACCGA 1 cut(s) 1268
TatI WGTACW 3 cut(s) 681, 2061, 2950
TauI GCSGC 1 cut(s) 816
Tru1I TTAA 9 cut(s) 69, 285, 596, 1439, 1760, 1947, 1989, 2399, 2547
Tru9I TTAA 9 cut(s) 69, 285, 596, 1439, 1760, 1947, 1989, 2399, 2547
TscAI CASTG 3 cut(s) 2223, 2502, 2651
TseFI GTSAC 5 cut(s) 43, 784, 1386, 2035, 2084
TseI GCWGC 8 cut(s) 347, 989, 1367, 1831, 2070, 2578, 2675, 2904
Tsp45I GTSAC 5 cut(s) 43, 784, 1386, 2035, 2084
TspDTI ATGAA 9 cut(s) 165, 461, 866, 1017, 2201, 2723, 2769, 2808, 2862
TspGWI ACGGA 2 cut(s) 197, 819
TspRI CASTG 3 cut(s) 2223, 2502, 2651
Tth111I GACNNNGTC 1 cut(s) 782
VneI GTGCAC 1 cut(s) 849
VpaK11BI GGWCC 5 cut(s) 224, 1465, 2213, 2486, 2516
XapI RAATTY 3 cut(s) 462, 1256, 1821
XceI RCATGY 1 cut(s) 545
XmiI GTMKAC 2 cut(s) 258, 801
XmnI GAANNNNTTC 1 cut(s) 518
XspI CTAG 6 cut(s) 86, 1334, 1349, 1523, 2057, 2393
Zsp2I ATGCAT 1 cut(s) 1680
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.