RchiOBHm_Chr5g0079351

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
85027267 .. 85033741
6475 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35377

Sequence Viewer

Length: 3468 bp
ATGGAGTTCGTCGAGATGTCGTTGGCGATTATTGTGGTAATTGTGACATGGATCGGTGCCATAGCTACTATTAAGTGGATATGGACTCTACTGAAGCATTGGTTCGGTTACATGGTTGAAAGGCGTGAGCCAATTGGCATCGCTACTGAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGGATTACAGTCGACATACAGGATCGACTTAATAGGAGAGGAATTAAAACGTTCATGGATGATCGAGATCTTGAAGTAGGGAATGTTATTAGTCCCGCTCTCTTAACAGCAATCAGAGAATCAAAGTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTTTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTTTGCATGGAAGACAACAGAATTTTGCCACTTTTTTATGATGTTGATCCTGCTTATGTTCGATATCAGAACGGGAGATACGGAGATGCTTTCTCTAAGCATGAAACCTCTGAGCGACATGAATCAAAGAAGGTGAAGCAATGGAGAGCTGCTTTAAAAAAAGTGGCTGACATTTCTGGGTGGAATACAAATGATTATAAGACTCACAAAGAACTTGTTGACATCATTGTGGAATCTCTCTGCAGTAAAGTACCACCTGATGCAATTGAATCCACAGGAGATTTCCAAGCATATGAAGCAACAACAGAAGCCATGAATAAGGTTATGAAGGAGCTAACAGACAGCGAGATCAGTGCCGTTGGTGTCTACGGCATGGGCGGCGTTGGAAAGACAAGCATGGCGAGACATGTAGCTGCAAAAGCCTGCAAAAACGGTACTTTTAACCGTTGGATTATAGTTGCCATATCCCAAAGCCCCGACTTGAGAAAAATTCAAGACACATTGGCAGATTTCTTGGGCGTTAAATTGGACAAGGAGACAGAAGATGGAAGAGCAGCTGCGCTGCATAAGGAGATAATGAGAAGAGGAAGACTCCTGATAATCTTGGACGATGTTTGGAACAGAATAGAATTGTCAGCCATAGGAATTCCCAGCTACGAGGAGCTTCAAAAGTGCAATTCCAAAGTCGTACTCACCACACGGAGAAGGAATGTCTGTCATGTCATGAAGTGCCAAGCAAGCATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGACCTTGTTTCTGAGAAATGCAGTAATAAATCCTTTTCAGTCCACCACATTTGAGAATGTGGCGAGGAGGGTAGCTGTAGAATGTAAGGGTCTACCGATTGCTTTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCTGGGGGAATGGGAAAAGGCAGCTGAACTACTAGAGCAGTCGCGATGTGCCAATCCTAACCATGAGGATGAAAATGCATTCAATTGTATCAGATTAAGCTATGATTACTTGAAAAGTGAAGACCGCAAGTCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGACCATGAAATCGAAATGGAAGACTTGTTCAGGTATGCGATCGGAAAAGGATTGTTTCAAGATGCCGAAACATTGGTAAAAGCCAGAGGAACAGTAGTTTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATGGCGAGAAAAATGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCAAAATCTGAAGATGGGCATCGGTTTTTGGTAAAAGCTGGCTGTGGTTTAGAGAATTGGAGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTGATGAGGAACGAAATTCGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAACCGGAATGCTGATTTAGATGAGATCCCAGAAAAGTCCATCCAAAAGCTGAATGAATTAAGGCTCTTGGATCTTAGCGACACTAGTATTTCCGTGTTACCGCAGTCATTCATTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTCTCCCAAGAAATTTATTGACATTTCTATAGTGGGAAAACTGAAGCTTGAAATTCTTAGTATGAGAGAATGTCCCGGGAGCGGGGGATTGTCGAGATTTGGACATGAACATCCCGAGAGGAGGGAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAATCGCTCTTGGGCAAGAGCCTCGGTGAGTGGTGTGGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTAGAAGAAGAAACCAATATTTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATAGTGCAGGTTGGCATATCTGATGCAAAATACATCCCTAAAAATGTTGAGGCCAAACCAGATTGGGATTACTTTTATATAAGTATCATCGGCGGACGCAACTCCGGTGCATACAGAAGCGGAGATCATAATTCAAGATCCTTGTATCTTAGAGAAGCAACCATAAGTACCTTGCCTGAATGGTTTATCAACGCGGTGACAAAGAAAACGGAGACGCTAGAGTATGATTGCTGCAAAGGGATGAGTGACATTCTTATAGAATATGACCGTGGGATGTTACATAAACTCAAGCATCTCACAGTAGCTGGTCAAATTCTTCATTCCTATGTGTACTTGAAAGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCGTCTGTTAGCACTGATCCACCTTGAGGAGTTGTGTGTTGGTGATTTACCACTTGGGTCTTTCTCCAATATGAAGAGATTGCATATGTTTGGTTGTTTAATCTTGAAGAATGTATCAAAATTGGTACAAAGACTACCAAATCTGGAGAAACTAGATCTAAATTGGATGCCTAAATTGGAATATGTGTTTGGATGGGAAGGGTTTGAGCTGAAACAATCAAAACTGAGAGAGATGCATTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGAGGTCCTGCCCCACGCATAATGTTTCAGAGTGTTAAAAGTTTGTTCTTTTACCGTTGCGAGTTGCTGCAAAGTCTGTTCGCATGTGATGTAGCCGAGTGTCTTGTTCAATTGGAAGACCTTTTTGTAGAGCGGTGCCATTTGTTGAAAAGAGTAATTGAAGCAGTGAACAACGAGAAGATGGTTCTACCAAAATTGAAGAACCTAGTTTTGAAGAATCTTCCTATGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCATTCACTGGAGCACTTGATTGTGGTGGATTGCCCCCAGTTTCCATTTTCAACTTCTTCCGATCTCTTCGAATCCTTCGAAAGCAGGAACCATATTTCATTTTCAACCCCCGCTTCTGACTGCTTTGGCAGCACGAACCCAGTCAAACTCAATGATCGACAACTGTACAGCTTCCTATATGATAGAACTTCTGGTTTCCAATTGTGA

Protein Analysis

1155

Amino Acids

131.53

Weight (kDa)

6.25

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 52 - 216 1.9e-46 TIR domain
TIR_2 PF13676 55 - 163 1.3e-14 TIR domain
NB-ARC PF00931 235 - 398 1.7e-31 NB-ARC domain
LRR_8 PF13855 608 - 665 2.3e-08 Leucine rich repeat
LRR_14 PF23598 617 - 777 3.1e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 906 - 1045 3.4e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 986 - 1100 1.1e-10 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 594
Acc36I ACCTGC 1 cut(s) 2323
AccB1I GGYRCC 2 cut(s) 56, 3132
AccBSI CCGCTC 3 cut(s) 296, 2073, 3130
AccI GTMKAC 3 cut(s) 210, 762, 1264
AccII CGCG 3 cut(s) 1354, 1743, 2519
AclI AACGTT 1 cut(s) 248
AclWI GGATC 7 cut(s) 59, 228, 437, 1864, 1923, 2457, 2737
AcuI CTGAAG 3 cut(s) 113, 1704, 2054
AfiI CCNNNNNNNGG 5 cut(s) 2072, 2073, 2112, 2151, 2752
AflIII ACRYGT 1 cut(s) 802
AhdI GACNNNNNGTC 1 cut(s) 1438
AhlI ACTAGT 1 cut(s) 1928
AjnI CCWGG 1 cut(s) 1311
AjuI GAANNNNNNNTTGG 4 cut(s) 1122, 1154, 2898, 2930
AloI GAACNNNNNNTCC 2 cut(s) 1484, 1516
Alw21I GWGCWC 1 cut(s) 3276
Alw26I GTCTC 3 cut(s) 793, 926, 2531
AlwI GGATC 7 cut(s) 59, 228, 437, 1864, 1923, 2457, 2737
AlwNI CAGNNNCTG 1 cut(s) 2630
Ama87I CYCGRG 2 cut(s) 2066, 2105
AoxI GGCC 2 cut(s) 1736, 2376
AspLEI GCGC 1 cut(s) 958
AspS9I GGNCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
AsuC2I CCSGG 3 cut(s) 1655, 2067, 2068
AsuHPI GGTGA 5 cut(s) 541, 1081, 2203, 2533, 2780
AsuII TTCGAA 2 cut(s) 3330, 3339
AvaI CYCGRG 2 cut(s) 2066, 2105
AvaII GGWCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
BaeGI GKGCMC 1 cut(s) 1297
BanI GGYRCC 2 cut(s) 56, 3132
BbsI GAAGAC 6 cut(s) 414, 991, 1437, 1479, 1500, 3120
Bbv12I GWGCWC 1 cut(s) 3276
BccI CCATC 7 cut(s) 935, 1607, 1682, 1892, 2221, 2913, 3172
BceAI ACGGC 2 cut(s) 737, 781
BcgI CGANNNNNNTGC 4 cut(s) 731, 765, 2169, 2203
BciT130I CCWGG 1 cut(s) 1313
BciVI GTATCC 1 cut(s) 1651
BcnI CCSGG 3 cut(s) 1655, 2067, 2068
BcoDI GTCTC 3 cut(s) 793, 926, 2531
BcuI ACTAGT 1 cut(s) 1928
BfaI CTAG 6 cut(s) 1343, 1599, 1929, 2543, 2879, 3203
BfmI CTRYAG 3 cut(s) 637, 1248, 2019
BfuAI ACCTGC 1 cut(s) 2323
BfuI GTATCC 1 cut(s) 1651
BglII AGATCT 2 cut(s) 265, 2881
Bme1390I CCNGG 4 cut(s) 1313, 1655, 2067, 2068
Bme18I GGWCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
BmeRI GACNNNNNGTC 1 cut(s) 1438
BmeT110I CYCGRG 2 cut(s) 2066, 2105
BmgT120I GGNCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
BmiI GGNNCC 5 cut(s) 58, 183, 2973, 3134, 3350
BmrFI CCNGG 4 cut(s) 1313, 1655, 2067, 2068
BmrI ACTGGG 2 cut(s) 3292, 3395
BmuI ACTGGG 2 cut(s) 3292, 3395
BpiI GAAGAC 6 cut(s) 414, 991, 1437, 1479, 1500, 3120
BplI GAGNNNNNCTC 2 cut(s) 972, 1004
BpmI CTGGAG 3 cut(s) 1814, 2891, 3290
Bpu14I TTCGAA 2 cut(s) 3330, 3339
BpuEI CTTGAG 3 cut(s) 898, 2597, 2771
BpuMI CCSGG 3 cut(s) 1655, 2067, 2068
BsaBI GATNNNNATC 4 cut(s) 264, 441, 1692, 2166
BsaJI CCNNGG 4 cut(s) 1312, 2066, 2187, 2593
BsaWI WCCGGW 2 cut(s) 1848, 2429
Bsc4I CCNNNNNNNGG 5 cut(s) 2072, 2073, 2112, 2151, 2752
Bse1I ACTGG 4 cut(s) 2702, 3273, 3298, 3401
Bse3DI GCAATG 1 cut(s) 542
Bse8I GATNNNNATC 4 cut(s) 264, 441, 1692, 2166
BseBI CCWGG 1 cut(s) 1313
BseDI CCNNGG 4 cut(s) 1312, 2066, 2187, 2593
BseJI GATNNNNATC 4 cut(s) 264, 441, 1692, 2166
BseLI CCNNNNNNNGG 5 cut(s) 2072, 2073, 2112, 2151, 2752
BseMI GCAATG 1 cut(s) 542
BseMII CTCAG 4 cut(s) 138, 498, 1175, 2942
BseNI ACTGG 4 cut(s) 2702, 3273, 3298, 3401
BseRI GAGGAG 4 cut(s) 1070, 1252, 2125, 2768
BseSI GKGCMC 1 cut(s) 1297
BseYI CCCAGC 1 cut(s) 1046
BsgI GTGCAG 2 cut(s) 1744, 2351
Bsh1236I CGCG 3 cut(s) 1354, 1743, 2519
Bsh1285I CGRYCG 1 cut(s) 1515
BshFI GGCC 2 cut(s) 1738, 2378
BshNI GGYRCC 2 cut(s) 56, 3132
BsiEI CGRYCG 1 cut(s) 1515
BsiHKAI GWGCWC 1 cut(s) 3276
BsiHKCI CYCGRG 2 cut(s) 2066, 2105
BsiSI CCGG 4 cut(s) 1654, 1849, 2067, 2430
BslFI GGGAC 2 cut(s) 276, 2049
BslI CCNNNNNNNGG 5 cut(s) 2072, 2073, 2112, 2151, 2752
BsmAI GTCTC 3 cut(s) 793, 926, 2531
BsmBI CGTCTC 1 cut(s) 2531
BsmFI GGGAC 2 cut(s) 276, 2049
BsmI GAATGC 3 cut(s) 1137, 1388, 1858
BsnI GGCC 2 cut(s) 1738, 2378
BsoBI CYCGRG 2 cut(s) 2066, 2105
Bsp119I TTCGAA 2 cut(s) 3330, 3339
Bsp1286I GDGCHC 2 cut(s) 1297, 3276
Bsp1407I TGTACA 2 cut(s) 2253, 3426
Bsp68I TCGCGA 1 cut(s) 1354
BspANI GGCC 2 cut(s) 1738, 2378
BspCNI CTCAG 4 cut(s) 139, 499, 1176, 2943
BspFNI CGCG 3 cut(s) 1354, 1743, 2519
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 5 cut(s) 58, 183, 2973, 3134, 3350
BspMAI CTGCAG 1 cut(s) 641
BspMI ACCTGC 1 cut(s) 2323
BspPI GGATC 7 cut(s) 59, 228, 437, 1864, 1923, 2457, 2737
BspQI GCTCTTC 2 cut(s) 940, 2981
BspT104I TTCGAA 2 cut(s) 3330, 3339
BspT107I GGYRCC 2 cut(s) 56, 3132
BsrBI CCGCTC 3 cut(s) 296, 2073, 3130
BsrDI GCAATG 1 cut(s) 542
BsrGI TGTACA 2 cut(s) 2253, 3426
BsrI ACTGG 4 cut(s) 2702, 3273, 3298, 3401
BssECI CCNNGG 4 cut(s) 1312, 2066, 2187, 2593
Bst2UI CCWGG 1 cut(s) 1313
Bst6I CTCTTC 7 cut(s) 940, 973, 1803, 2715, 2795, 2981, 3332
BstAPI GCANNNNNTGC 1 cut(s) 1443
BstAUI TGTACA 2 cut(s) 2253, 3426
BstBI TTCGAA 2 cut(s) 3330, 3339
BstC8I GCNNGC 5 cut(s) 820, 1135, 1604, 1714, 1796
BstDSI CCRYGG 1 cut(s) 2593
BstFNI CGCG 3 cut(s) 1354, 1743, 2519
BstHHI GCGC 1 cut(s) 958
BstMAI GTCTC 3 cut(s) 793, 926, 2531
BstMCI CGRYCG 1 cut(s) 1515
BstMWI GCNNNNNNNGC 7 cut(s) 692, 774, 815, 1134, 1280, 1443, 3390
BstNI CCWGG 1 cut(s) 1313
BstNSI RCATGY 2 cut(s) 806, 3084
BstSCI CCNGG 4 cut(s) 1311, 1653, 2065, 2066
BstSFI CTRYAG 3 cut(s) 637, 1248, 2019
BstSLI GKGCMC 1 cut(s) 1297
BstUI CGCG 3 cut(s) 1354, 1743, 2519
BstV2I GAAGAC 6 cut(s) 414, 991, 1437, 1479, 1500, 3120
BstX2I RGATCY 5 cut(s) 265, 1869, 1915, 2462, 2881
BstYI RGATCY 5 cut(s) 265, 1869, 1915, 2462, 2881
BsuI GTATCC 1 cut(s) 1651
BsuRI GGCC 2 cut(s) 1738, 2378
BtgI CCRYGG 1 cut(s) 2593
BtgZI GCGATG 2 cut(s) 124, 1369
BtsI GCAGTG 2 cut(s) 1761, 3168
BtsIMutI CAGTG 9 cut(s) 754, 1581, 1761, 1772, 2709, 2738, 2988, 3168, 3266
BtuMI TCGCGA 1 cut(s) 1354
BveI ACCTGC 1 cut(s) 2323
Cac8I GCNNGC 5 cut(s) 820, 1135, 1604, 1714, 1796
CaiI CAGNNNCTG 1 cut(s) 2630
CciI TCATGA 1 cut(s) 1119
CfoI GCGC 1 cut(s) 958
Cfr13I GGNCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
Cfr9I CCCGGG 1 cut(s) 2066
CseI GACGC 3 cut(s) 2430, 2548, 2717
CspCI CAANNNNNGTGG 2 cut(s) 1204, 1239
DraI TTTAAA 1 cut(s) 552
DriI GACNNNNNGTC 1 cut(s) 1438
Eam1104I CTCTTC 7 cut(s) 940, 973, 1803, 2715, 2795, 2981, 3332
Eam1105I GACNNNNNGTC 1 cut(s) 1438
EarI CTCTTC 7 cut(s) 940, 973, 1803, 2715, 2795, 2981, 3332
EciI GGCGGA 1 cut(s) 2433
Eco32I GATATC 2 cut(s) 461, 2226
Eco47I GGWCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
Eco57I CTGAAG 3 cut(s) 113, 1704, 2054
Eco88I CYCGRG 2 cut(s) 2066, 2105
EcoO109I RGGNCCY 3 cut(s) 182, 1309, 3002
EcoRI GAATTC 1 cut(s) 1041
EcoRII CCWGG 1 cut(s) 1311
EcoRV GATATC 2 cut(s) 461, 2226
EcoT22I ATGCAT 3 cut(s) 1390, 1644, 2964
Esp3I CGTCTC 1 cut(s) 2531
FalI AAGNNNNNCTT 2 cut(s) 1430, 1462
FaqI GGGAC 2 cut(s) 276, 2049
FauI CCCGC 3 cut(s) 301, 2066, 3379
FauNDI CATATG 2 cut(s) 688, 2811
FblI GTMKAC 3 cut(s) 210, 762, 1264
FspBI CTAG 6 cut(s) 1343, 1599, 1929, 2543, 2879, 3203
GlaI GCGC 1 cut(s) 957
GsaI CCCAGC 1 cut(s) 1050
GsuI CTGGAG 3 cut(s) 1814, 2891, 3290
HaeIII GGCC 2 cut(s) 1738, 2378
HapII CCGG 4 cut(s) 1654, 1849, 2067, 2430
HgaI GACGC 3 cut(s) 2430, 2548, 2717
HhaI GCGC 1 cut(s) 958
Hin6I GCGC 1 cut(s) 956
HinP1I GCGC 1 cut(s) 956
HincII GTYRAC 2 cut(s) 211, 616
HindII GTYRAC 2 cut(s) 211, 616
HindIII AAGCTT 2 cut(s) 1977, 2036
HpaII CCGG 4 cut(s) 1654, 1849, 2067, 2430
HphI GGTGA 5 cut(s) 541, 1081, 2203, 2533, 2780
Hpy166II GTNNAC 8 cut(s) 211, 616, 763, 1215, 1265, 2263, 2656, 3166
Hpy8I GTNNAC 8 cut(s) 211, 616, 763, 1215, 1265, 2263, 2656, 3166
Hpy99I CGWCG 1 cut(s) 14
HpyAV CCTTC 6 cut(s) 520, 718, 1095, 1748, 2918, 3346
HpyCH4IV ACGT 1 cut(s) 248
HpyF10VI GCNNNNNNNGC 7 cut(s) 692, 774, 815, 1134, 1280, 1443, 3390
HpySE526I ACGT 1 cut(s) 248
HspAI GCGC 1 cut(s) 956
LguI GCTCTTC 2 cut(s) 940, 2981
LmnI GCTCC 4 cut(s) 727, 1057, 2070, 3271
MaeI CTAG 6 cut(s) 1343, 1599, 1929, 2543, 2879, 3203
MaeII ACGT 1 cut(s) 248
MaeIII GTNAC 7 cut(s) 43, 107, 1941, 2203, 2521, 2570, 2601
MbiI CCGCTC 3 cut(s) 296, 2073, 3130
MfeI CAATTG 5 cut(s) 132, 660, 1393, 3107, 3461
MflI RGATCY 5 cut(s) 265, 1869, 1915, 2462, 2881
MhlI GDGCHC 2 cut(s) 1297, 3276
MlyI GAGTC 3 cut(s) 79, 592, 981
MmeI TCCRAC 2 cut(s) 760, 824
Mph1103I ATGCAT 3 cut(s) 1390, 1644, 2964
MslI CAYNNNNRTG 3 cut(s) 623, 1377, 2649
MspA1I CMGCKG 2 cut(s) 953, 1334
MspI CCGG 4 cut(s) 1654, 1849, 2067, 2430
MspR9I CCNGG 4 cut(s) 1313, 1655, 2067, 2068
MunI CAATTG 5 cut(s) 132, 660, 1393, 3107, 3461
Mva1269I GAATGC 3 cut(s) 1137, 1388, 1858
MvaI CCWGG 1 cut(s) 1313
MvnI CGCG 3 cut(s) 1354, 1743, 2519
MwoI GCNNNNNNNGC 7 cut(s) 692, 774, 815, 1134, 1280, 1443, 3390
NciI CCSGG 3 cut(s) 1655, 2067, 2068
NdeI CATATG 2 cut(s) 688, 2811
NlaIV GGNNCC 5 cut(s) 58, 183, 2973, 3134, 3350
NmeAIII GCCGAG 1 cut(s) 3118
NmuCI GTSAC 4 cut(s) 43, 2203, 2521, 2570
NruI TCGCGA 1 cut(s) 1354
NsiI ATGCAT 3 cut(s) 1390, 1644, 2964
NspI RCATGY 2 cut(s) 806, 3084
NspV TTCGAA 2 cut(s) 3330, 3339
PagI TCATGA 1 cut(s) 1119
PciI ACATGT 1 cut(s) 802
PciSI GCTCTTC 2 cut(s) 940, 2981
PcsI WCGNNNNNNNCGW 1 cut(s) 3336
PctI GAATGC 3 cut(s) 1137, 1388, 1858
PfeI GAWTC 9 cut(s) 317, 518, 629, 665, 1166, 1595, 1991, 3214, 3332
PfoI TCCNGGA 1 cut(s) 1653
Ple19I CGATCG 1 cut(s) 1515
PleI GAGTC 3 cut(s) 79, 592, 981
PpsI GAGTC 3 cut(s) 79, 592, 981
PpuMI RGGWCCY 3 cut(s) 182, 1309, 3002
PscI ACATGT 1 cut(s) 802
PsiI TTATAA 1 cut(s) 594
Psp1406I AACGTT 1 cut(s) 248
Psp5II RGGWCCY 3 cut(s) 182, 1309, 3002
Psp6I CCWGG 1 cut(s) 1311
PspFI CCCAGC 1 cut(s) 1046
PspGI CCWGG 1 cut(s) 1311
PspN4I GGNNCC 5 cut(s) 58, 183, 2973, 3134, 3350
PspPI GGNCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
PspPPI RGGWCCY 3 cut(s) 182, 1309, 3002
PsrI GAACNNNNNNTAC 2 cut(s) 458, 490
PstI CTGCAG 1 cut(s) 641
PstNI CAGNNNCTG 1 cut(s) 2630
PsuI RGATCY 5 cut(s) 265, 1869, 1915, 2462, 2881
PvuI CGATCG 1 cut(s) 1515
PvuII CAGCTG 2 cut(s) 953, 1334
RruI TCGCGA 1 cut(s) 1354
RseI CAYNNNNRTG 3 cut(s) 623, 1377, 2649
SalI GTCGAC 1 cut(s) 209
SapI GCTCTTC 2 cut(s) 940, 2981
Sau96I GGNCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
SchI GAGTC 3 cut(s) 79, 592, 981
ScrFI CCNGG 4 cut(s) 1313, 1655, 2067, 2068
SduI GDGCHC 2 cut(s) 1297, 3276
SfcI CTRYAG 3 cut(s) 637, 1248, 2019
SfuI TTCGAA 2 cut(s) 3330, 3339
SinI GGWCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
SmaI CCCGGG 1 cut(s) 2068
SmiMI CAYNNNNRTG 3 cut(s) 623, 1377, 2649
SmlI CTYRAG 3 cut(s) 877, 2612, 2750
SmoI CTYRAG 3 cut(s) 877, 2612, 2750
SpeI ACTAGT 1 cut(s) 1928
SspI AATATT 1 cut(s) 2290
SspMI CTAG 6 cut(s) 1343, 1599, 1929, 2543, 2879, 3203
StyD4I CCNGG 4 cut(s) 1311, 1653, 2065, 2066
TaiI ACGT 1 cut(s) 251
TatI WGTACW 4 cut(s) 2253, 2262, 2655, 3426
TauI GCSGC 1 cut(s) 777
TfiI GAWTC 9 cut(s) 317, 518, 629, 665, 1166, 1595, 1991, 3214, 3332
TscAI CASTG 9 cut(s) 754, 1581, 1768, 1779, 2709, 2745, 2988, 3168, 3273
TseFI GTSAC 4 cut(s) 43, 2203, 2521, 2570
Tsp45I GTSAC 4 cut(s) 43, 2203, 2521, 2570
TspGWI ACGGA 4 cut(s) 492, 1111, 1927, 2549
TspMI CCCGGG 1 cut(s) 2066
TspRI CASTG 9 cut(s) 754, 1581, 1768, 1779, 2709, 2745, 2988, 3168, 3273
VpaK11BI GGWCC 6 cut(s) 182, 1173, 1309, 2699, 2972, 3002
XceI RCATGY 2 cut(s) 806, 3084
XmaI CCCGGG 1 cut(s) 2066
XmiI GTMKAC 3 cut(s) 210, 762, 1264
XspI CTAG 6 cut(s) 1343, 1599, 1929, 2543, 2879, 3203
Zsp2I ATGCAT 3 cut(s) 1390, 1644, 2964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.