FvH4_3g45170

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37548313 .. 37552989
4677 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45170.t3

Sequence Viewer

Length: 2853 bp
ATGGCCTCCTCTGCATCTCTTCTATCATCCGCTGAATCATCAACCGCTCGTTTGTCGATGCACGGTGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTACATCTGAATTATATGAGCGGCTGCATAAACAAAGAGGAATCAAAACATTCATGGATGATCAAGATCTTGAAGTAGGAGATCCTATATCTCCCACTCTACTAAAAGCAATTGAAGAATCAAGGTTTGCAATTGTTGTCCTTTCTCCAAACTATGCCTCTTCTACTTGGTGTTTGGAGGAACTTGCGAAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTCTTCCACTTTTCTATAATGTCGAGCCTACTGATGTAAGACATCAAAAGGGAAGTTTTGAAGAAGCTTTCAGCAAATACGAGAGGCGTAGATCAGAGAAGGTCCAGCAGTGGAGAGATGCTTTAAACAAAGTGGCCAGTTTCACTGGGTGGCATACAAAGAATTATAAGACTGATAGAGAACTTGTGGAGGAAATTGTGGATTCTGTGTGCAACAGAATACAACCTTTTGAAATTGACTCGACATTGGCCACCGGAGATTTTGAAGAGTTTGAAGCAACAAAAAAAGCCATGGATCAGGTTATGAAGGTGCTTCAAGATGTTGACGTCCCTGCCGTCGGGGTCTACGGAATGGGAGGCGTCGGCAAGACAACCCTGGTAAAACATGTTGGTGCAGAAGCCCGAAAAACAGGGATTATTCATTATGCGGTTATGGCTGTTCTATCCCAAAGCCCAGACATAGCAAAGATTCAAGGCACATTGGCAGATATGTTGGACGTTAAATTGGAGGGAGAGACAGAATTTGGAAGAGCTGTTAGATTGAAGAGAGAGATAATGAGAAGAGAAAAAATCCTTATAATCTTGGACGATCTATGGGATAAAATAGAGTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAAAGTTCAAGTCCAAAGTGCTATTCACAACAAGGAAATTAAAAGTCTGTCATGCCATGAAGTGCCAAGAAAAGATCAACCTCAACATTCTCTCAGAAAGAGATTCTTGGAACTTGTTTCTGAGAAAAGCAGGAACAATTCCTCTCGAGTCCCAGAACGTGGCGAGGAAGGTAGCTGGAGAATGTAAGGGTCTACCGATTGCATTAATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCAGGAGGAATGGGATGAGGCAGCTAAACGACTAGAGCAGTCCAAATATGTCAATCCCGACCATGAGGACGATGAGGAAAATGCATTCAGATGTATACGATTAAGCTATGATTACTTGAAAAATGAAGACCACAAGGCATGCTTCTTGCTCTGTTGCTTGTTCCCAGAAGACGATGACATCAAAATAGAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGATAATACAATGGAATTAGCCAGAGGAAGAGTAGTTACAGTGGTGAAGTACCTGAAAGGTTCGAGCTTGCTTTTGGATAGCGAGCAAAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCGAAATCTGAAGATGGGTTTTGGGTGAAAGCTGGCTATGGTTTAAAGGGTTGGCCACACGGATTAGATGAGGATTGCACTGCTATTTCACTAATGAGGAACACAATTCGCAAGCTACCTGAAGAAGAGTTGGTTGGCTTATCCGATGAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATAACTATCAACAGCAGATGCACCAACACTCGATCCGCGTCCCTTGCATACAAACAAGGAGATCGTAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATAACTGCTGCGGGGGGATGAGTGACATTGTTATGGAATATGACCATTGGAGGTTACATAAACTCAAGCATCTCACAGTTGCTTGGAACAAGTTGAAAGAGTTGATGAACACAACAAGACGAGTTGAAACAGGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGTATCTGAAGCACCCGGTGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCTAATCTCAAGGTGTTTCATGTGTATCAGATGATTTTGAGGAGTACGATTTTGAGGAGTGTATCAAAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGTTTTAAAATCTCTGTATAAATTGGAATATGTGTTCAGATGCGAAGGGGTCGAGCCAGAACAATCAAAACTGAGAGAGATGCATTTGTTGTGGCTATTTTCACTAAGAAGCATATGTAGTGGTCCTGCCCCACGTGCAATGTTCCAGAGTCTTCAGATTTTGACGATTTACCGTTGCCAGCGGCTGCAAAGTCTGTTCGCATATGATGTGGCTGAGTGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAACTTGGTTTTGAAGAATCTTCCTATTTTGTATGGTGCAATTGCTGCCGTTGATATTGAGTGTCCTTCATTGGAACACTTGATCGTGGTGGATTGCCCCCATATTCCATTTTCAACCACTTCCTATCTCTTCTACTCCTTAGAATCCAAAAGCAGGAGCCGGTATTCATTTTCATCCTCCGCTTCTGACTACTTTGGCAGCACGTACCCAGTCCAACTTAATGATCCAGAATCGTACAACTTTTTAAGTGGCAGGTATGTCTCTGTGTGA

Protein Analysis

951

Amino Acids

108.54

Weight (kDa)

6.21

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 183 1e-52 TIR domain
TIR_2 PF13676 23 - 119 3.1e-17 TIR domain
NB-ARC PF00931 204 - 369 6.2e-29 NB-ARC domain
LRR_RPS2 PF23247 699 - 840 8.2e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 495, 905
AatII GACGTC 1 cut(s) 657
Acc36I ACCTGC 1 cut(s) 2826
AccB7I CCANNNNNTGG 1 cut(s) 1132
AccBSI CCGCTC 2 cut(s) 47, 127
AccI GTMKAC 3 cut(s) 672, 1165, 1306
AccII CGCG 2 cut(s) 1418, 1820
AciI CCGC 9 cut(s) 30, 45, 127, 755, 1818, 1913, 1953, 2446, 2763
AclWI GGATC 5 cut(s) 182, 630, 1809, 1851, 2801
AcoI YGGCCR 3 cut(s) 462, 576, 1634
AcsI RAATTY 4 cut(s) 327, 848, 2089, 2231
AcuI CTGAAG 5 cut(s) 1611, 1722, 2135, 2402, 2600
AcvI CACGTG 1 cut(s) 2399
AcyI GRCGYC 2 cut(s) 654, 687
AdeI CACNNNGTG 3 cut(s) 67, 477, 2125
AfaI GTAC 7 cut(s) 1415, 1493, 1888, 2209, 2238, 2789, 2819
AfiI CCNNNNNNNGG 4 cut(s) 665, 1132, 2527, 2736
AflIII ACRYGT 1 cut(s) 712
AhlI ACTAGT 1 cut(s) 2263
AjnI CCWGG 3 cut(s) 702, 1212, 2152
AjuI GAANNNNNNNTTGG 4 cut(s) 980, 1012, 1698, 1730
AloI GAACNNNNNNTCC 2 cut(s) 2282, 2314
Alw26I GTCTC 4 cut(s) 836, 2163, 2236, 2848
AlwI GGATC 5 cut(s) 182, 630, 1809, 1851, 2801
AlwNI CAGNNNCTG 1 cut(s) 2449
Ama87I CYCGRG 1 cut(s) 1118
AoxI GGCC 4 cut(s) 3, 462, 576, 1634
ApeKI GCWGC 6 cut(s) 130, 1232, 1950, 2449, 2627, 2781
ApoI RAATTY 4 cut(s) 327, 848, 2089, 2231
AseI ATTAAT 1 cut(s) 1178
Asp700I GAANNNNTTC 1 cut(s) 2502
AspS9I GGNCC 5 cut(s) 86, 430, 1210, 2075, 2387
AsuC2I CCSGG 2 cut(s) 1562, 2123
AsuHPI GGTGA 4 cut(s) 1498, 1618, 1927, 2153
AvaI CYCGRG 1 cut(s) 1118
AvaII GGWCC 5 cut(s) 86, 430, 1210, 2075, 2387
BaeGI GKGCMC 1 cut(s) 1198
BalI TGGCCA 3 cut(s) 464, 578, 1636
BarI GAAGNNNNNNTAC 4 cut(s) 91, 123, 1462, 1494
BbrPI CACGTG 1 cut(s) 2399
BbsI GAAGAC 6 cut(s) 1344, 1386, 1407, 2408, 2505, 2567
BbvI GCAGC 6 cut(s) 117, 1244, 1937, 2436, 2614, 2793
BccI CCATC 2 cut(s) 1589, 1889
BceAI ACGGC 2 cut(s) 647, 2615
BciT130I CCWGG 3 cut(s) 704, 1214, 2154
BciVI GTATCC 1 cut(s) 1558
BclI TGATCA 1 cut(s) 166
BcnI CCSGG 2 cut(s) 1562, 2123
BcoDI GTCTC 4 cut(s) 836, 2163, 2236, 2848
BcuI ACTAGT 1 cut(s) 2263
BfaI CTAG 3 cut(s) 1244, 1937, 2264
BfuAI ACCTGC 1 cut(s) 2826
BfuI GTATCC 1 cut(s) 1558
BglII AGATCT 2 cut(s) 172, 297
BisI GCNGC 8 cut(s) 128, 131, 1233, 1951, 2447, 2450, 2628, 2782
BlsI GCNGC 8 cut(s) 129, 132, 1234, 1952, 2448, 2451, 2629, 2783
Bme1390I CCNGG 5 cut(s) 704, 1214, 1562, 2123, 2154
Bme18I GGWCC 5 cut(s) 86, 430, 1210, 2075, 2387
BmeT110I CYCGRG 1 cut(s) 1118
BmgT120I GGNCC 5 cut(s) 86, 430, 1210, 2075, 2387
BmiI GGNNCC 2 cut(s) 87, 2741
BmrFI CCNGG 5 cut(s) 704, 1214, 1562, 2123, 2154
BmrI ACTGGG 2 cut(s) 483, 2786
BmsI GCATC 9 cut(s) 23, 48, 436, 1536, 1790, 2020, 2113, 2294, 2334
BmuI ACTGGG 2 cut(s) 483, 2786
BpiI GAAGAC 6 cut(s) 1344, 1386, 1407, 2408, 2505, 2567
BpmI CTGGAG 2 cut(s) 1170, 2276
BpuEI CTTGAG 2 cut(s) 1991, 2156
BpuMI CCSGG 2 cut(s) 1562, 2123
BsaAI YACGTR 2 cut(s) 2399, 2787
BsaBI GATNNNNATC 1 cut(s) 171
BsaHI GRCGYC 2 cut(s) 654, 687
BsaI GGTCTC 1 cut(s) 2163
BsaJI CCNNGG 3 cut(s) 618, 702, 2153
BsaWI WCCGGW 2 cut(s) 581, 1410
Bsc4I CCNNNNNNNGG 4 cut(s) 665, 1132, 2527, 2736
Bse118I RCCGGY 1 cut(s) 2742
Bse1I ACTGG 4 cut(s) 465, 478, 2078, 2792
Bse3DI GCAATG 1 cut(s) 2409
Bse8I GATNNNNATC 1 cut(s) 171
BseBI CCWGG 3 cut(s) 704, 1214, 2154
BseDI CCNNGG 3 cut(s) 618, 702, 2153
BseGI GGATG 8 cut(s) 26, 169, 1231, 1541, 1557, 1737, 1965, 2756
BseJI GATNNNNATC 1 cut(s) 171
BseLI CCNNNNNNNGG 4 cut(s) 665, 1132, 2527, 2736
BseMI GCAATG 1 cut(s) 2409
BseMII CTCAG 4 cut(s) 1080, 1085, 2327, 2469
BseNI ACTGG 4 cut(s) 465, 478, 2078, 2792
BseRI GAGGAG 2 cut(s) 2218, 2233
BseSI GKGCMC 1 cut(s) 1198
BseXI GCAGC 6 cut(s) 117, 1244, 1937, 2436, 2614, 2793
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 741
Bsh1236I CGCG 2 cut(s) 1418, 1820
Bsh1285I CGRYCG 1 cut(s) 1422
BshFI GGCC 4 cut(s) 5, 464, 578, 1636
BsiEI CGRYCG 1 cut(s) 1422
BsiHKCI CYCGRG 1 cut(s) 1118
BsiSI CCGG 5 cut(s) 582, 1411, 1561, 2123, 2743
BslFI GGGAC 3 cut(s) 641, 1108, 1807
BslI CCNNNNNNNGG 4 cut(s) 665, 1132, 2527, 2736
BsmAI GTCTC 4 cut(s) 836, 2163, 2236, 2848
BsmFI GGGAC 3 cut(s) 641, 1108, 1807
BsmI GAATGC 1 cut(s) 1295
BsnI GGCC 4 cut(s) 5, 464, 578, 1636
Bso31I GGTCTC 1 cut(s) 2163
BsoBI CYCGRG 1 cut(s) 1118
Bsp1286I GDGCHC 1 cut(s) 1198
Bsp1407I TGTACA 1 cut(s) 2236
Bsp19I CCATGG 1 cut(s) 618
BspACI CCGC 9 cut(s) 30, 45, 127, 755, 1818, 1913, 1953, 2446, 2763
BspANI GGCC 4 cut(s) 5, 464, 578, 1636
BspCNI CTCAG 4 cut(s) 1079, 1086, 2328, 2470
BspFNI CGCG 2 cut(s) 1418, 1820
BspLI GGNNCC 2 cut(s) 87, 2741
BspMI ACCTGC 1 cut(s) 2826
BspPI GGATC 5 cut(s) 182, 630, 1809, 1851, 2801
BspQI GCTCTTC 1 cut(s) 850
BspTNI GGTCTC 1 cut(s) 2163
BsrBI CCGCTC 2 cut(s) 47, 127
BsrDI GCAATG 1 cut(s) 2409
BsrFI RCCGGY 1 cut(s) 2742
BsrGI TGTACA 1 cut(s) 2236
BsrI ACTGG 4 cut(s) 465, 478, 2078, 2792
BssAI RCCGGY 1 cut(s) 2742
BssECI CCNNGG 3 cut(s) 618, 702, 2153
BssNAI GTATAC 1 cut(s) 1307
BssNI GRCGYC 2 cut(s) 654, 687
BssT1I CCWWGG 1 cut(s) 618
Bst1107I GTATAC 1 cut(s) 1307
Bst2UI CCWGG 3 cut(s) 704, 1214, 2154
Bst4CI ACNGT 5 cut(s) 65, 1483, 2020, 2438, 2566
BstACI GRCGYC 2 cut(s) 654, 687
BstAPI GCANNNNNTGC 1 cut(s) 2627
BstAUI TGTACA 1 cut(s) 2236
BstBAI YACGTR 2 cut(s) 2399, 2787
BstC8I GCNNGC 6 cut(s) 1351, 1511, 1526, 1615, 1694, 2444
BstDEI CTNAG 6 cut(s) 1066, 1094, 2336, 2369, 2478, 2722
BstDSI CCRYGG 1 cut(s) 618
BstF5I GGATG 8 cut(s) 26, 169, 1231, 1541, 1557, 1737, 1965, 2756
BstFNI CGCG 2 cut(s) 1418, 1820
BstMAI GTCTC 4 cut(s) 836, 2163, 2236, 2848
BstMCI CGRYCG 1 cut(s) 1422
BstMWI GCNNNNNNNGC 6 cut(s) 11, 761, 1181, 1826, 2399, 2627
BstNI CCWGG 3 cut(s) 704, 1214, 2154
BstNSI RCATGY 2 cut(s) 716, 1353
BstSCI CCNGG 5 cut(s) 702, 1212, 1560, 2121, 2152
BstSLI GKGCMC 1 cut(s) 1198
BstUI CGCG 2 cut(s) 1418, 1820
BstV1I GCAGC 6 cut(s) 117, 1244, 1937, 2436, 2614, 2793
BstV2I GAAGAC 6 cut(s) 1344, 1386, 1407, 2408, 2505, 2567
BstX2I RGATCY 4 cut(s) 172, 187, 297, 1856
BstYI RGATCY 4 cut(s) 172, 187, 297, 1856
BstZ17I GTATAC 1 cut(s) 1307
BsuI GTATCC 1 cut(s) 1558
BsuRI GGCC 4 cut(s) 5, 464, 578, 1636
BtgI CCRYGG 1 cut(s) 618
BtsCI GGATG 8 cut(s) 26, 169, 1231, 1541, 1557, 1737, 1965, 2756
BtsI GCAGTG 3 cut(s) 443, 1659, 2553
BtsIMutI CAGTG 6 cut(s) 443, 471, 1488, 1659, 2085, 2553
BveI ACCTGC 1 cut(s) 2826
Cac8I GCNNGC 6 cut(s) 1351, 1511, 1526, 1615, 1694, 2444
CaiI CAGNNNCTG 1 cut(s) 2449
Cfr10I RCCGGY 1 cut(s) 2742
Cfr13I GGNCC 5 cut(s) 86, 430, 1210, 2075, 2387
CseI GACGC 2 cut(s) 676, 1809
Csp6I GTAC 7 cut(s) 1414, 1492, 1887, 2208, 2237, 2788, 2818
CviQI GTAC 7 cut(s) 1414, 1492, 1887, 2208, 2237, 2788, 2818
DdeI CTNAG 6 cut(s) 1066, 1094, 2336, 2369, 2478, 2722
DraI TTTAAA 3 cut(s) 453, 1626, 2271
DraIII CACNNNGTG 3 cut(s) 67, 477, 2125
EaeI YGGCCR 3 cut(s) 462, 576, 1634
Eco130I CCWWGG 1 cut(s) 618
Eco31I GGTCTC 1 cut(s) 2163
Eco47I GGWCC 5 cut(s) 86, 430, 1210, 2075, 2387
Eco57I CTGAAG 5 cut(s) 1611, 1722, 2135, 2402, 2600
Eco72I CACGTG 1 cut(s) 2399
Eco88I CYCGRG 1 cut(s) 1118
EcoO109I RGGNCCY 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 3 cut(s) 702, 1212, 2152
EcoT14I CCWWGG 1 cut(s) 618
EcoT22I ATGCAT 3 cut(s) 1297, 1551, 2349
ErhI CCWWGG 1 cut(s) 618
FalI AAGNNNNNCTT 6 cut(s) 1063, 1095, 1337, 1369, 2490, 2522
FaqI GGGAC 3 cut(s) 641, 1108, 1807
FauI CCCGC 1 cut(s) 1946
FauNDI CATATG 2 cut(s) 2378, 2467
FbaI TGATCA 1 cut(s) 166
FblI GTMKAC 3 cut(s) 672, 1165, 1306
Fnu4HI GCNGC 8 cut(s) 128, 131, 1233, 1951, 2447, 2450, 2628, 2782
FokI GGATG 8 cut(s) 13, 176, 1238, 1544, 1548, 1724, 1972, 2743
Fsp4HI GCNGC 8 cut(s) 128, 131, 1233, 1951, 2447, 2450, 2628, 2782
FspBI CTAG 3 cut(s) 1244, 1937, 2264
GluI GCNGC 8 cut(s) 128, 131, 1233, 1951, 2447, 2450, 2628, 2782
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 2 cut(s) 1170, 2276
HaeIII GGCC 4 cut(s) 5, 464, 578, 1636
HapII CCGG 5 cut(s) 582, 1411, 1561, 2123, 2743
HgaI GACGC 2 cut(s) 676, 1809
Hin1I GRCGYC 2 cut(s) 654, 687
HincII GTYRAC 1 cut(s) 652
HindII GTYRAC 1 cut(s) 652
HindIII AAGCTT 1 cut(s) 393
HpaII CCGG 5 cut(s) 582, 1411, 1561, 2123, 2743
HphI GGTGA 4 cut(s) 1498, 1618, 1927, 2153
Hpy166II GTNNAC 5 cut(s) 652, 673, 1166, 1307, 2551
Hpy8I GTNNAC 5 cut(s) 652, 673, 1166, 1307, 2551
Hpy99I CGWCG 2 cut(s) 668, 692
HpyAV CCTTC 7 cut(s) 93, 421, 628, 1135, 2303, 2513, 2658
HpyCH4III ACNGT 5 cut(s) 65, 1483, 2020, 2438, 2566
HpyCH4IV ACGT 5 cut(s) 654, 825, 1131, 2398, 2786
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 761, 1181, 1826, 2399, 2627
HpyF3I CTNAG 6 cut(s) 1066, 1094, 2336, 2369, 2478, 2722
HpySE526I ACGT 5 cut(s) 654, 825, 1131, 2398, 2786
Hsp92I GRCGYC 2 cut(s) 654, 687
Ksp22I TGATCA 1 cut(s) 166
LguI GCTCTTC 1 cut(s) 850
LmnI GCTCC 3 cut(s) 967, 1874, 2739
Lsp1109I GCAGC 6 cut(s) 117, 1244, 1937, 2436, 2614, 2793
LweI GCATC 9 cut(s) 23, 48, 436, 1536, 1790, 2020, 2113, 2294, 2334
MaeI CTAG 3 cut(s) 1244, 1937, 2264
MaeII ACGT 5 cut(s) 654, 825, 1131, 2398, 2786
MaeIII GTNAC 5 cut(s) 1477, 1915, 1964, 1995, 2145
MbiI CCGCTC 2 cut(s) 47, 127
MfeI CAATTG 4 cut(s) 216, 237, 2492, 2622
MflI RGATCY 4 cut(s) 172, 187, 297, 1856
MhlI GDGCHC 1 cut(s) 1198
MlsI TGGCCA 3 cut(s) 464, 578, 1636
MluNI TGGCCA 3 cut(s) 464, 578, 1636
MlyI GAGTC 3 cut(s) 560, 1130, 2422
MmeI TCCRAC 3 cut(s) 801, 2508, 2821
Mox20I TGGCCA 3 cut(s) 464, 578, 1636
Mph1103I ATGCAT 3 cut(s) 1297, 1551, 2349
MroXI GAANNNNTTC 1 cut(s) 2502
MscI TGGCCA 3 cut(s) 464, 578, 1636
MslI CAYNNNNRTG 3 cut(s) 717, 1300, 1973
Msp20I TGGCCA 3 cut(s) 464, 578, 1636
MspA1I CMGCKG 2 cut(s) 32, 2446
MspI CCGG 5 cut(s) 582, 1411, 1561, 2123, 2743
MspR9I CCNGG 5 cut(s) 704, 1214, 1562, 2123, 2154
MunI CAATTG 4 cut(s) 216, 237, 2492, 2622
Mva1269I GAATGC 1 cut(s) 1295
MvaI CCWGG 3 cut(s) 704, 1214, 2154
MvnI CGCG 2 cut(s) 1418, 1820
MwoI GCNNNNNNNGC 6 cut(s) 11, 761, 1181, 1826, 2399, 2627
NciI CCSGG 2 cut(s) 1562, 2123
NcoI CCATGG 1 cut(s) 618
NdeI CATATG 2 cut(s) 2378, 2467
NlaIV GGNNCC 2 cut(s) 87, 2741
NmuCI GTSAC 2 cut(s) 1915, 1964
NsiI ATGCAT 3 cut(s) 1297, 1551, 2349
NspI RCATGY 2 cut(s) 716, 1353
PaeI GCATGC 1 cut(s) 1353
PaeR7I CTCGAG 1 cut(s) 1118
PciI ACATGT 1 cut(s) 712
PciSI GCTCTTC 1 cut(s) 850
PcsI WCGNNNNNNNCGW 1 cut(s) 660
PctI GAATGC 1 cut(s) 1295
PdmI GAANNNNTTC 1 cut(s) 2502
PflMI CCANNNNNTGG 1 cut(s) 1132
PfoI TCCNGGA 1 cut(s) 1560
PkrI GCNGC 8 cut(s) 129, 132, 1234, 1952, 2448, 2451, 2629, 2783
Ple19I CGATCG 1 cut(s) 1422
PleI GAGTC 3 cut(s) 560, 1129, 2421
PmaCI CACGTG 1 cut(s) 2399
PmlI CACGTG 1 cut(s) 2399
PpsI GAGTC 3 cut(s) 560, 1129, 2421
Ppu21I YACGTR 2 cut(s) 2399, 2787
PpuMI RGGWCCY 1 cut(s) 86
PscI ACATGT 1 cut(s) 712
PshBI ATTAAT 1 cut(s) 1178
PsiI TTATAA 2 cut(s) 495, 905
Psp5II RGGWCCY 1 cut(s) 86
Psp6I CCWGG 3 cut(s) 702, 1212, 2152
PspCI CACGTG 1 cut(s) 2399
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 3 cut(s) 702, 1212, 2152
PspN4I GGNNCC 2 cut(s) 87, 2741
PspPI GGNCC 5 cut(s) 86, 430, 1210, 2075, 2387
PspPPI RGGWCCY 1 cut(s) 86
PstNI CAGNNNCTG 1 cut(s) 2449
PsuI RGATCY 4 cut(s) 172, 187, 297, 1856
PvuI CGATCG 1 cut(s) 1422
RsaI GTAC 7 cut(s) 1415, 1493, 1888, 2209, 2238, 2789, 2819
RsaNI GTAC 7 cut(s) 1414, 1492, 1887, 2208, 2237, 2788, 2818
RseI CAYNNNNRTG 3 cut(s) 717, 1300, 1973
SapI GCTCTTC 1 cut(s) 850
SatI GCNGC 8 cut(s) 128, 131, 1233, 1951, 2447, 2450, 2628, 2782
Sau96I GGNCC 5 cut(s) 86, 430, 1210, 2075, 2387
SchI GAGTC 3 cut(s) 560, 1130, 2422
ScrFI CCNGG 5 cut(s) 704, 1214, 1562, 2123, 2154
SduI GDGCHC 1 cut(s) 1198
SfaNI GCATC 9 cut(s) 23, 48, 436, 1536, 1790, 2020, 2113, 2294, 2334
Sfr274I CTCGAG 1 cut(s) 1118
SinI GGWCC 5 cut(s) 86, 430, 1210, 2075, 2387
SlaI CTCGAG 1 cut(s) 1118
SmiMI CAYNNNNRTG 3 cut(s) 717, 1300, 1973
SmlI CTYRAG 3 cut(s) 1118, 2006, 2171
SmoI CTYRAG 3 cut(s) 1118, 2006, 2171
SpeI ACTAGT 1 cut(s) 2263
SphI GCATGC 1 cut(s) 1353
SsiI CCGC 9 cut(s) 30, 45, 127, 755, 1818, 1913, 1953, 2446, 2763
SspMI CTAG 3 cut(s) 1244, 1937, 2264
StyD4I CCNGG 5 cut(s) 702, 1212, 1560, 2121, 2152
StyI CCWWGG 1 cut(s) 618
TaaI ACNGT 5 cut(s) 65, 1483, 2020, 2438, 2566
TaiI ACGT 5 cut(s) 657, 828, 1134, 2401, 2789
TaqI TCGA 9 cut(s) 56, 97, 351, 569, 1119, 1438, 1505, 1813, 2316
TatI WGTACW 1 cut(s) 2236
TauI GCSGC 2 cut(s) 130, 2449
TscAI CASTG 6 cut(s) 443, 478, 1488, 1666, 2085, 2553
TseFI GTSAC 2 cut(s) 1915, 1964
TseI GCWGC 6 cut(s) 130, 1232, 1950, 2449, 2627, 2781
Tsp45I GTSAC 2 cut(s) 1915, 1964
TspGWI ACGGA 2 cut(s) 690, 1656
TspRI CASTG 6 cut(s) 443, 478, 1488, 1666, 2085, 2553
Van91I CCANNNNNTGG 1 cut(s) 1132
VpaK11BI GGWCC 5 cut(s) 86, 430, 1210, 2075, 2387
VspI ATTAAT 1 cut(s) 1178
XapI RAATTY 4 cut(s) 327, 848, 2089, 2231
XceI RCATGY 2 cut(s) 716, 1353
XhoI CTCGAG 1 cut(s) 1118
XmiI GTMKAC 3 cut(s) 672, 1165, 1306
XmnI GAANNNNTTC 1 cut(s) 2502
XspI CTAG 3 cut(s) 1244, 1937, 2264
ZraI GACGTC 1 cut(s) 655
Zsp2I ATGCAT 3 cut(s) 1297, 1551, 2349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.