Rmu_sc0001148.1_g000022

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001148.1
OG0000379
Physical Location & Seq
Forward (+)
79775 .. 85519
5745 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001148.1_g000022.1.cds

Sequence Viewer

Length: 3696 bp
atgtcgttggcgattattctggtaattgtgacatggatcggtgccatagctactattaagtggatatggactctactgaagcattggttcggttacatggttgaaaggcgtgagccagtatcgcaggatattcaagaactgattgacattgctactgagcaagtaaggcccgagagagaagaagaagaagaaaagatgtgggatctccatgactctcctgatcagggaaacgatggcaccacctcgaccgccgcgccagttgctccttcctcttctctatcatcagctgttgagtggaagtatgatgtgtttttgagtttcaggggtcctgacactcgcaagggtatcacatttgaactatatgatcgattacagaggaggggaattaagacattcatggatgatcgagatcttgaagtagggaatgttatttctccaactctcttaagggcaattagagaatcaaggtttgcgattgttgttctctcgcaaaattatgcttcttctacttggtgtttggaggaacttagagagatttgtctgtgcatggaagacaacagaattttgcctctcttttatcatgttggtcctactgatgttcgatatcagaagaggagtttcaaagatgctttctctaagcatgaaacctctgagcgacatgaatcagagaaggtgaagcaatggagagctgctttatacaaagtggccaatatctctgggtggaatacaaatgattataagactcaccaagaacttgttgacgtcattgtggaatctctccacagtaaagtaccacctgatgcaattgaatccacaggagatttccaagcatacaaagcaacaacacaagccatggatgaggttaagaaggcgctaacagatgacgagatcactgccgttggtgtctacggcatgggcggcgttggaaagacaagcatggtgagacatgtagctgcacaagcctgcaaaaatggcacttttaaccattggattatggctgccatatcccaaagcccagactttagaaacattcaaggtacattggcagattttttgggcgttaaatttgtggaggagacagaaagtggaagagctgataggttgcataaggagataatgagaagagaaaaactcctgataatcttggacgatgcttgggagagaatagaattttcagccataggaattcccagctacgagaagcttcaaaagtgcagatccaaagtcgtactcaccacacggaaattgcatgtctgtgattccatggacagccaacgaagcattcctctcaatatcttatcagaacaagattcttggaccttgtttgtgcgaaatgcaggggtagcgaagaagattcttgaatcgaccacttttaaggatgtggcaaggagagtatctggagaatgcaggggtctacccattgcattgatagcagttgcaagggcactcagagataaagatcaggaggaatgggataaagcagctcgaagactagagaagtcacaatctgcaatcgttgaggatgagaaagaggccttcaaatgtataaaatttagctatgattacttgaaagatgaggattccaagtcatgcttcttgctctgttgcttatacccagaagaccatgacatcctcgtagaacagttattcaggtgtgcaattgggaaaggattgtttcgagatgccgaaacaatcgaagaagccagaggaacagcagattcagtggtcaagtacctgaaagattctagcttgcttttggatagcgagaaaaaaggatgtgtaaagatgcatgatgtcatccgagatacagccctgaatattgcaaaatctgaagatgggcacgggtttctggtaagagctggctgtggtttaaaggattggccacgcggattacatgaaggctgcactgctatttcactaatgaggaacagcattcgcacgctccccaaagaagagttggtatgtccaaatctccagattttatttctacaagagaatactgatttaaccgagatcccagaaaagtttatccaaagtctgaatgaattaagggtgttggatcttagcttcacttatatttcctctttaccccaatcattcagcctccttaccaacctccaagctttgaatttagatttttgcaagaaattgattgacatttccgtactcggaaaacttaaaaggcttgaaattcttagtatgaggaattatcgtgggaaggaattgtcgacagaaatgggacatttgacaaatctaaggattttggaagtcagttgcgctgcatctctcataattccatctaaagtgatactgaagttgcataaattagaagaactgtatacacaaaactgtgaatttgaggattgggggagtaaagttgagagagaaggagaagaaactgaactggtcttccctgttttgtcaaatttgaaaatctggcgtattcgcacacctggatacaggggtgaaggtgttaacgatcgatatggtcgtaactcaagatccttgtttctttttgacggatcaaccatgagtaccttacctgattggtttatcaacgtggtgacagagaaaacagaggagctagagtatcgtagctgcaaagggatgagtgacattcttacggaatatgaccatgggaggttacatgaactaaagcatctccgagtagactatgatgatattattgctttcgaggtgggcatggtggacatgaaagagttgatgaacacaacaagacgagttctaaaaggaccagtgtttgagaatttggaagagttgcatctgatggctctgtacccccttgaggagttgtgtgttggtgagttcccacctgggtcgtctttctcaaatcttaaggtatttcatctgtttttgatttataacttggggaatgtatcaaattttttgaagagactacccaatctggagaagctatatttaaactgtgtgggtaaatttgaatatgtctttgaatgcgaagggtttgagccgaaacaatcaaaactgagagagatgtgtttggcgggtctagaagtgaagggcatttatagtggtcctgccccgcgtgcaatgtttcagagtcttaaaaatctgaccatttacgattgcaagttgctgcaaagtctgttcgcatctgatgtagctgagtgtctctttcaattggaagacctttatgtagagcggtgccctttgttggaaagagtaattgaagcagtgaacaaggataaggcggttctaccaaaattgaagaacttggtttttgagaatcttcctatgttgtatggtgcaagtgctactgttgatattgagtgtccttcattggaacacttgattgtcgtggattgctcccagttttcattttcaacttcttactatgtcttcgatttcgaaagcaggaaccggttttcattttcaacccctccttctgactacttttgcgccaggaacccagtcaaactcaatgatcgacaactgtacagatctctacgttacagagcaggaaccaaatctggccaactccatgataaaagaaatcttcaatttctttgtgaagggttggctgaggctgcaaatccaattgacacactagaagcttgctggatcgtgacttccaaaacagccaaagggattgtaacagccattcttggaacctttgagtgtgaagagatcagtggacgtgctcaatcaggatga

Protein Analysis

1231

Amino Acids

140.4

Weight (kDa)

5.6

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 738, 2883
AatII GACGTC 1 cut(s) 765
AccB1I GGYRCC 3 cut(s) 41, 236, 3186
AccBSI CCGCTC 1 cut(s) 3184
AccI GTMKAC 5 cut(s) 906, 1414, 2228, 2339, 2670
AccII CGCG 3 cut(s) 254, 1887, 3067
AciI CCGC 8 cut(s) 249, 252, 918, 1887, 3026, 3065, 3184, 3233
AclWI GGATC 8 cut(s) 44, 210, 1212, 2008, 2067, 2496, 2530, 3611
AcoI YGGCCR 3 cut(s) 705, 1880, 3514
AcuI CTGAAG 3 cut(s) 98, 1851, 2333
AcyI GRCGYC 1 cut(s) 762
AfaI GTAC 8 cut(s) 792, 1039, 1230, 1733, 2166, 2536, 2798, 3479
AfiI CCNNNNNNNGG 3 cut(s) 224, 2806, 3196
AflII CTTAAG 2 cut(s) 445, 2855
AflIII ACRYGT 1 cut(s) 946
AgeI ACCGGT 1 cut(s) 3402
AjiI CACGTC 1 cut(s) 3680
AjnI CCWGG 3 cut(s) 2452, 2833, 3443
AjuI GAANNNNNNNTTGG 4 cut(s) 1266, 1298, 1577, 1609
AloI GAACNNNNNNTCC 2 cut(s) 2394, 2426
Alw21I GWGCWC 1 cut(s) 3685
Alw26I GTCTC 4 cut(s) 937, 1070, 2908, 3158
AlwI GGATC 8 cut(s) 44, 210, 1212, 2008, 2067, 2496, 2530, 3611
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 5 cut(s) 167, 705, 1533, 1880, 3514
ApeKI GCWGC 9 cut(s) 689, 953, 998, 1481, 1902, 2279, 2598, 3118, 3569
AsiGI ACCGGT 1 cut(s) 3402
AspLEI GCGC 4 cut(s) 256, 874, 2279, 3443
AspS9I GGNCC 6 cut(s) 168, 326, 587, 1317, 2753, 3056
AsuHPI GGTGA 7 cut(s) 685, 737, 952, 1225, 2477, 2575, 2834
AsuII TTCGAA 1 cut(s) 3390
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 5 cut(s) 326, 587, 1317, 2753, 3056
BaeGI GKGCMC 3 cut(s) 1447, 1842, 3191
BaeI ACNNNNGTAYC 4 cut(s) 2300, 2333, 2449, 2482
BalI TGGCCA 3 cut(s) 707, 1882, 3516
BanI GGYRCC 3 cut(s) 41, 236, 3186
BbsI GAAGAC 6 cut(s) 558, 1495, 1626, 2401, 3174, 3373
Bbv12I GWGCWC 1 cut(s) 3685
BbvCI CCTCAGC 1 cut(s) 3564
BbvI GCAGC 9 cut(s) 676, 940, 985, 1493, 1889, 2266, 2585, 3105, 3556
BccI CCATC 4 cut(s) 227, 1829, 2305, 2782
BceAI ACGGC 2 cut(s) 881, 925
BcgI CGANNNNNNTGC 2 cut(s) 875, 909
BciT130I CCWGG 3 cut(s) 2454, 2835, 3445
BciVI GTATCC 1 cut(s) 2450
BclI TGATCA 1 cut(s) 220
BcoDI GTCTC 4 cut(s) 937, 1070, 2908, 3158
BfaI CTAG 5 cut(s) 1493, 1746, 2585, 3032, 3590
BfoI RGCGCY 1 cut(s) 875
BfrI CTTAAG 2 cut(s) 445, 2855
BfuI GTATCC 1 cut(s) 2450
BglII AGATCT 2 cut(s) 409, 3482
Bme1390I CCNGG 3 cut(s) 2454, 2835, 3445
Bme18I GGWCC 5 cut(s) 326, 587, 1317, 2753, 3056
BmeT110I CYCGRG 1 cut(s) 170
BmgBI CACGTC 1 cut(s) 3680
BmgT120I GGNCC 6 cut(s) 168, 326, 587, 1317, 2753, 3056
BmiI GGNNCC 8 cut(s) 43, 238, 327, 3188, 3401, 3449, 3505, 3652
BmrFI CCNGG 3 cut(s) 2454, 2835, 3445
BmrI ACTGGG 2 cut(s) 3346, 3446
BmsI GCATC 9 cut(s) 616, 790, 1141, 1672, 1776, 2291, 2668, 2791, 3143
BmuI ACTGGG 2 cut(s) 3346, 3446
BpiI GAAGAC 6 cut(s) 558, 1495, 1626, 2401, 3174, 3373
BplI GAGNNNNNCTC 2 cut(s) 1116, 1148
BpmI CTGGAG 3 cut(s) 1419, 1958, 2948
Bpu10I CCTNAGC 1 cut(s) 3564
Bpu14I TTCGAA 1 cut(s) 3390
BpuEI CTTGAG 2 cut(s) 2482, 2825
Bsa29I ATCGAT 2 cut(s) 367, 2482
BsaBI GATNNNNATC 2 cut(s) 408, 1458
BsaHI GRCGYC 1 cut(s) 762
BsaJI CCNNGG 4 cut(s) 852, 1263, 2635, 2834
BsaWI WCCGGW 1 cut(s) 3402
BsaXI ACNNNNNCTCC 2 cut(s) 2573, 2603
Bsc4I CCNNNNNNNGG 3 cut(s) 224, 2806, 3196
Bse118I RCCGGY 1 cut(s) 3402
Bse1I ACTGG 6 cut(s) 116, 257, 2409, 2756, 3352, 3452
Bse3DI GCAATG 4 cut(s) 147, 686, 1419, 3078
Bse8I GATNNNNATC 2 cut(s) 408, 1458
BseBI CCWGG 3 cut(s) 2454, 2835, 3445
BseCI ATCGAT 2 cut(s) 367, 2482
BseDI CCNNGG 4 cut(s) 852, 1263, 2635, 2834
BseGI GGATG 8 cut(s) 406, 862, 1384, 1528, 1629, 1781, 1797, 2613
BseJI GATNNNNATC 2 cut(s) 408, 1458
BseLI CCNNNNNNNGG 3 cut(s) 224, 2806, 3196
BseMI GCAATG 4 cut(s) 147, 686, 1419, 3078
BseMII CTCAG 6 cut(s) 147, 642, 1462, 2999, 3138, 3555
BseNI ACTGG 6 cut(s) 116, 257, 2409, 2756, 3352, 3452
BseRI GAGGAG 5 cut(s) 391, 628, 1088, 2594, 2822
BseSI GKGCMC 3 cut(s) 1447, 1842, 3191
BseXI GCAGC 9 cut(s) 676, 940, 985, 1493, 1889, 2266, 2585, 3105, 3556
BseYI CCCAGC 1 cut(s) 1190
BsgI GTGCAG 3 cut(s) 939, 1234, 1888
Bsh1236I CGCG 3 cut(s) 254, 1887, 3067
Bsh1285I CGRYCG 2 cut(s) 249, 2482
BshFI GGCC 5 cut(s) 169, 707, 1535, 1882, 3516
BshNI GGYRCC 3 cut(s) 41, 236, 3186
BshTI ACCGGT 1 cut(s) 3402
BshVI ATCGAT 2 cut(s) 367, 2482
BsiEI CGRYCG 2 cut(s) 249, 2482
BsiHKAI GWGCWC 1 cut(s) 3685
BsiHKCI CYCGRG 1 cut(s) 170
BsiSI CCGG 1 cut(s) 3403
BslFI GGGAC 1 cut(s) 2253
BslI CCNNNNNNNGG 3 cut(s) 224, 2806, 3196
BsmAI GTCTC 4 cut(s) 937, 1070, 2908, 3158
BsmFI GGGAC 1 cut(s) 2253
BsmI GAATGC 4 cut(s) 1281, 1409, 1932, 2981
BsnI GGCC 5 cut(s) 169, 707, 1535, 1882, 3516
BsoBI CYCGRG 1 cut(s) 170
Bsp119I TTCGAA 1 cut(s) 3390
Bsp1286I GDGCHC 4 cut(s) 1447, 1842, 3191, 3685
Bsp1407I TGTACA 1 cut(s) 3477
Bsp19I CCATGG 3 cut(s) 852, 1263, 2635
BspACI CCGC 8 cut(s) 249, 252, 918, 1887, 3026, 3065, 3184, 3233
BspANI GGCC 5 cut(s) 169, 707, 1535, 1882, 3516
BspCNI CTCAG 6 cut(s) 148, 643, 1461, 3000, 3139, 3556
BspDI ATCGAT 2 cut(s) 367, 2482
BspFNI CGCG 3 cut(s) 254, 1887, 3067
BspLI GGNNCC 8 cut(s) 43, 238, 327, 3188, 3401, 3449, 3505, 3652
BspPI GGATC 8 cut(s) 44, 210, 1212, 2008, 2067, 2496, 2530, 3611
BspQI GCTCTTC 1 cut(s) 1084
BspT104I TTCGAA 1 cut(s) 3390
BspT107I GGYRCC 3 cut(s) 41, 236, 3186
BspTI CTTAAG 2 cut(s) 445, 2855
BsrBI CCGCTC 1 cut(s) 3184
BsrDI GCAATG 4 cut(s) 147, 686, 1419, 3078
BsrFI RCCGGY 1 cut(s) 3402
BsrGI TGTACA 1 cut(s) 3477
BsrI ACTGG 6 cut(s) 116, 257, 2409, 2756, 3352, 3452
BssAI RCCGGY 1 cut(s) 3402
BssECI CCNNGG 4 cut(s) 852, 1263, 2635, 2834
BssNAI GTATAC 1 cut(s) 2340
BssNI GRCGYC 1 cut(s) 762
BssT1I CCWWGG 3 cut(s) 852, 1263, 2635
Bst1107I GTATAC 1 cut(s) 2340
Bst2UI CCWGG 3 cut(s) 2454, 2835, 3445
Bst4CI ACNGT 7 cut(s) 785, 1644, 2337, 2351, 2948, 3301, 3477
Bst6I CTCTTC 8 cut(s) 277, 605, 1084, 1117, 1947, 2769, 2906, 3660
BstACI GRCGYC 1 cut(s) 762
BstAFI CTTAAG 2 cut(s) 445, 2855
BstAUI TGTACA 1 cut(s) 3477
BstBI TTCGAA 1 cut(s) 3390
BstC8I GCNNGC 6 cut(s) 964, 1751, 1861, 1940, 3069, 3598
BstDSI CCRYGG 3 cut(s) 852, 1263, 2635
BstF5I GGATG 8 cut(s) 406, 862, 1384, 1528, 1629, 1781, 1797, 2613
BstFNI CGCG 3 cut(s) 254, 1887, 3067
BstH2I RGCGCY 1 cut(s) 875
BstHHI GCGC 4 cut(s) 256, 874, 2279, 3443
BstMAI GTCTC 4 cut(s) 937, 1070, 2908, 3158
BstMCI CGRYCG 2 cut(s) 249, 2482
BstNI CCWGG 3 cut(s) 2454, 2835, 3445
BstNSI RCATGY 2 cut(s) 950, 1253
BstSCI CCNGG 3 cut(s) 2452, 2833, 3443
BstSLI GKGCMC 3 cut(s) 1447, 1842, 3191
BstUI CGCG 3 cut(s) 254, 1887, 3067
BstV1I GCAGC 9 cut(s) 676, 940, 985, 1493, 1889, 2266, 2585, 3105, 3556
BstV2I GAAGAC 6 cut(s) 558, 1495, 1626, 2401, 3174, 3373
BstX2I RGATCY 7 cut(s) 202, 409, 1217, 2013, 2059, 2501, 3482
BstYI RGATCY 7 cut(s) 202, 409, 1217, 2013, 2059, 2501, 3482
BstZ17I GTATAC 1 cut(s) 2340
Bsu15I ATCGAT 2 cut(s) 367, 2482
BsuI GTATCC 1 cut(s) 2450
BsuRI GGCC 5 cut(s) 169, 707, 1535, 1882, 3516
BsuTUI ATCGAT 2 cut(s) 367, 2482
BtgI CCRYGG 3 cut(s) 852, 1263, 2635
BtrI CACGTC 1 cut(s) 3680
BtsCI GGATG 8 cut(s) 406, 862, 1384, 1528, 1629, 1781, 1797, 2613
BtsI GCAGTG 3 cut(s) 891, 1905, 3222
BtsIMutI CAGTG 6 cut(s) 891, 1728, 1905, 2763, 3222, 3679
Cac8I GCNNGC 6 cut(s) 964, 1751, 1861, 1940, 3069, 3598
CfoI GCGC 4 cut(s) 256, 874, 2279, 3443
Cfr10I RCCGGY 1 cut(s) 3402
Cfr13I GGNCC 6 cut(s) 168, 326, 587, 1317, 2753, 3056
ClaI ATCGAT 2 cut(s) 367, 2482
Csp6I GTAC 8 cut(s) 791, 1038, 1229, 1732, 2165, 2535, 2797, 3478
CspAI ACCGGT 1 cut(s) 3402
CviQI GTAC 8 cut(s) 791, 1038, 1229, 1732, 2165, 2535, 2797, 3478
DraI TTTAAA 2 cut(s) 1872, 2943
EaeI YGGCCR 3 cut(s) 705, 1880, 3514
Eam1104I CTCTTC 8 cut(s) 277, 605, 1084, 1117, 1947, 2769, 2906, 3660
EarI CTCTTC 8 cut(s) 277, 605, 1084, 1117, 1947, 2769, 2906, 3660
Eco130I CCWWGG 3 cut(s) 852, 1263, 2635
Eco147I AGGCCT 1 cut(s) 1535
Eco32I GATATC 1 cut(s) 605
Eco47I GGWCC 5 cut(s) 326, 587, 1317, 2753, 3056
Eco57I CTGAAG 3 cut(s) 98, 1851, 2333
Eco88I CYCGRG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 326
EcoRI GAATTC 1 cut(s) 1185
EcoRII CCWGG 3 cut(s) 2452, 2833, 3443
EcoRV GATATC 1 cut(s) 605
EcoT14I CCWWGG 3 cut(s) 852, 1263, 2635
EcoT22I ATGCAT 1 cut(s) 1791
ErhI CCWWGG 3 cut(s) 852, 1263, 2635
FalI AAGNNNNNCTT 2 cut(s) 1577, 1609
FaqI GGGAC 1 cut(s) 2253
FauI CCCGC 2 cut(s) 3019, 3072
FbaI TGATCA 1 cut(s) 220
FblI GTMKAC 5 cut(s) 906, 1414, 2228, 2339, 2670
FokI GGATG 8 cut(s) 413, 869, 1391, 1535, 1616, 1784, 1788, 2620
FspBI CTAG 5 cut(s) 1493, 1746, 2585, 3032, 3590
GlaI GCGC 4 cut(s) 255, 873, 2278, 3442
GsaI CCCAGC 1 cut(s) 1194
GsuI CTGGAG 3 cut(s) 1419, 1958, 2948
HaeII RGCGCY 1 cut(s) 875
HaeIII GGCC 5 cut(s) 169, 707, 1535, 1882, 3516
HapII CCGG 1 cut(s) 3403
HhaI GCGC 4 cut(s) 256, 874, 2279, 3443
Hin1I GRCGYC 1 cut(s) 762
Hin6I GCGC 4 cut(s) 254, 872, 2277, 3441
HinP1I GCGC 4 cut(s) 254, 872, 2277, 3441
HincII GTYRAC 3 cut(s) 760, 2229, 2476
HindII GTYRAC 3 cut(s) 760, 2229, 2476
HindIII AAGCTT 3 cut(s) 1202, 2121, 3594
HpaI GTTAAC 1 cut(s) 2476
HpaII CCGG 1 cut(s) 3403
HphI GGTGA 7 cut(s) 685, 737, 952, 1225, 2477, 2575, 2834
HpyCH4III ACNGT 7 cut(s) 785, 1644, 2337, 2351, 2948, 3301, 3477
HpyCH4IV ACGT 4 cut(s) 762, 2559, 3490, 3679
HpySE526I ACGT 4 cut(s) 762, 2559, 3490, 3679
Hsp92I GRCGYC 1 cut(s) 762
HspAI GCGC 4 cut(s) 254, 872, 2277, 3441
Ksp22I TGATCA 1 cut(s) 220
KspAI GTTAAC 1 cut(s) 2476
LguI GCTCTTC 1 cut(s) 1084
LmnI GCTCC 4 cut(s) 268, 1947, 2581, 3353
Lsp1109I GCAGC 9 cut(s) 676, 940, 985, 1493, 1889, 2266, 2585, 3105, 3556
LweI GCATC 9 cut(s) 616, 790, 1141, 1672, 1776, 2291, 2668, 2791, 3143
MaeI CTAG 5 cut(s) 1493, 1746, 2585, 3032, 3590
MaeII ACGT 4 cut(s) 762, 2559, 3490, 3679
MbiI CCGCTC 1 cut(s) 3184
MfeI CAATTG 4 cut(s) 804, 1659, 3161, 3579
MflI RGATCY 7 cut(s) 202, 409, 1217, 2013, 2059, 2501, 3482
MhlI GDGCHC 4 cut(s) 1447, 1842, 3191, 3685
MlsI TGGCCA 3 cut(s) 707, 1882, 3516
MluNI TGGCCA 3 cut(s) 707, 1882, 3516
MlyI GAGTC 4 cut(s) 64, 206, 736, 3091
MmeI TCCRAC 4 cut(s) 461, 904, 2037, 3177
Mox20I TGGCCA 3 cut(s) 707, 1882, 3516
Mph1103I ATGCAT 1 cut(s) 1791
MscI TGGCCA 3 cut(s) 707, 1882, 3516
MslI CAYNNNNRTG 1 cut(s) 1254
Msp20I TGGCCA 3 cut(s) 707, 1882, 3516
MspA1I CMGCKG 1 cut(s) 287
MspCI CTTAAG 2 cut(s) 445, 2855
MspI CCGG 1 cut(s) 3403
MspR9I CCNGG 3 cut(s) 2454, 2835, 3445
MunI CAATTG 4 cut(s) 804, 1659, 3161, 3579
Mva1269I GAATGC 4 cut(s) 1281, 1409, 1932, 2981
MvaI CCWGG 3 cut(s) 2454, 2835, 3445
MvnI CGCG 3 cut(s) 254, 1887, 3067
NcoI CCATGG 3 cut(s) 852, 1263, 2635
NlaIV GGNNCC 8 cut(s) 43, 238, 327, 3188, 3401, 3449, 3505, 3652
NmuCI GTSAC 5 cut(s) 28, 1500, 2563, 2612, 3607
NsiI ATGCAT 1 cut(s) 1791
NspI RCATGY 2 cut(s) 950, 1253
NspV TTCGAA 1 cut(s) 3390
PceI AGGCCT 1 cut(s) 1535
PciI ACATGT 1 cut(s) 946
PciSI GCTCTTC 1 cut(s) 1084
PcsI WCGNNNNNNNCGW 1 cut(s) 2488
PctI GAATGC 4 cut(s) 1281, 1409, 1932, 2981
PinAI ACCGGT 1 cut(s) 3402
Ple19I CGATCG 1 cut(s) 2482
PleI GAGTC 4 cut(s) 64, 206, 736, 3090
PpsI GAGTC 4 cut(s) 64, 206, 736, 3090
PpuMI RGGWCCY 1 cut(s) 326
PscI ACATGT 1 cut(s) 946
PsiI TTATAA 2 cut(s) 738, 2883
Psp5II RGGWCCY 1 cut(s) 326
Psp6I CCWGG 3 cut(s) 2452, 2833, 3443
PspFI CCCAGC 1 cut(s) 1190
PspGI CCWGG 3 cut(s) 2452, 2833, 3443
PspN4I GGNNCC 8 cut(s) 43, 238, 327, 3188, 3401, 3449, 3505, 3652
PspPI GGNCC 6 cut(s) 168, 326, 587, 1317, 2753, 3056
PspPPI RGGWCCY 1 cut(s) 326
PsuI RGATCY 7 cut(s) 202, 409, 1217, 2013, 2059, 2501, 3482
PvuI CGATCG 1 cut(s) 2482
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 8 cut(s) 792, 1039, 1230, 1733, 2166, 2536, 2798, 3479
RsaNI GTAC 8 cut(s) 791, 1038, 1229, 1732, 2165, 2535, 2797, 3478
RseI CAYNNNNRTG 1 cut(s) 1254
SalI GTCGAC 1 cut(s) 2227
SapI GCTCTTC 1 cut(s) 1084
Sau96I GGNCC 6 cut(s) 168, 326, 587, 1317, 2753, 3056
SchI GAGTC 4 cut(s) 64, 206, 736, 3091
ScrFI CCNGG 3 cut(s) 2454, 2835, 3445
SduI GDGCHC 4 cut(s) 1447, 1842, 3191, 3685
SfaNI GCATC 9 cut(s) 616, 790, 1141, 1672, 1776, 2291, 2668, 2791, 3143
SfuI TTCGAA 1 cut(s) 3390
SinI GGWCC 5 cut(s) 326, 587, 1317, 2753, 3056
SmiMI CAYNNNNRTG 1 cut(s) 1254
SmlI CTYRAG 4 cut(s) 445, 2497, 2804, 2855
SmoI CTYRAG 4 cut(s) 445, 2497, 2804, 2855
SseBI AGGCCT 1 cut(s) 1535
SsiI CCGC 8 cut(s) 249, 252, 918, 1887, 3026, 3065, 3184, 3233
SspI AATATT 1 cut(s) 1819
SspMI CTAG 5 cut(s) 1493, 1746, 2585, 3032, 3590
StuI AGGCCT 1 cut(s) 1535
StyD4I CCNGG 3 cut(s) 2452, 2833, 3443
StyI CCWWGG 3 cut(s) 852, 1263, 2635
TaaI ACNGT 7 cut(s) 785, 1644, 2337, 2351, 2948, 3301, 3477
TaiI ACGT 4 cut(s) 765, 2562, 3493, 3682
TatI WGTACW 1 cut(s) 3477
TauI GCSGC 2 cut(s) 254, 921
TscAI CASTG 6 cut(s) 898, 1728, 1912, 2763, 3222, 3679
TseFI GTSAC 5 cut(s) 28, 1500, 2563, 2612, 3607
TseI GCWGC 9 cut(s) 689, 953, 998, 1481, 1902, 2279, 2598, 3118, 3569
Tsp45I GTSAC 5 cut(s) 28, 1500, 2563, 2612, 3607
TspGWI ACGGA 4 cut(s) 1255, 2152, 2535, 2639
TspRI CASTG 6 cut(s) 898, 1728, 1912, 2763, 3222, 3679
Vha464I CTTAAG 2 cut(s) 445, 2855
VpaK11BI GGWCC 5 cut(s) 326, 587, 1317, 2753, 3056
XbaI TCTAGA 1 cut(s) 3031
XceI RCATGY 2 cut(s) 950, 1253
XcmI CCANNNNNNNNNTGG 1 cut(s) 1954
XmiI GTMKAC 5 cut(s) 906, 1414, 2228, 2339, 2670
XspI CTAG 5 cut(s) 1493, 1746, 2585, 3032, 3590
ZraI GACGTC 1 cut(s) 763
Zsp2I ATGCAT 1 cut(s) 1791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.