Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0000379

Physical Location & Seq

Genomic Coordinates Forward (+)

37121833 .. 37125665

Gene Structure 3833 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g44561.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3336 bp

ATGGCCTCCTCCTCTTCATCATCGTCGATTCGCAGCTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTACTGACACTCGCAAAGGGATTGCAAACGAGATCTGCAATCGACTTAGTAGTAGAGGAATCAAAACGTTCATGGATGAGCAAGGACTTCAAGTAGGGGATGTTATTTCTCCCGCACTCATAAAGGCAATTAAAGAGTCAAGGTTTGCAATCGTAATTCTCTCGAAAAATTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATCCATGGAAGAGAGCAGAATTTTGCCACTTTTTTTTGAAGTTGATCCTACGGATGTCCGATTTCAGAAGAACAGTTTCAAAGAAGCTTTCTCTAAGCACGATACCTCTAAGCGACATGAATCAGAGAAGGTGCATCAATGGAGGGCTGCCTTAAGCAAAGTGGCCAATATCTCAGGGTGGAATACAAATGATTTCAAGACTCACAGTGAACTTGTTGACAAGATTGTGGAATCTCTCCTCAGTAAAGTACCACTTGATCCTATTGAATCCACAGGAGATTTCCAAGAATATGAAGCAACAAAAGAAGCCATGCGCAAGGTTATGGAGGCGCTAAAAGATGACAAGATTACTGTCGTTGGTGTCTATGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTACTTTTAACCATTGGATTATGGCTGTCATATCCCAAAACCTTGACTTGGAAAAAGTTCAAGGTACATTGGCAGATTTGTTGGGCTTTAAATTCGTGGAGGGGACAGAAGCTGGAAGAGCAAATAAGTTGCATAAGGAGATCATGAGAAAAGAAAAACTCCTGATAATTGTGGACGATGTTTGGGAGAGAATAGAGTTGTCAAAGATAGGGATTCCCACCTACAATGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCACCAGGAGATTGAATGTCTGTAATGCCATGAAGTGCCATGAAAAGATCGGCCTCAAAGTTCTCTCAGATCAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTTGAGCCCACCTCATTTGAGAAAGTGGCAAGGAGGGTAGCTTTAGAGTGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCAGGAGGAATGGAAGAAGGCAGTTGAACGACTAGAGCAGTCGCAATATGTCAATCCTAACCATGAGGAAGATGCATTCCGATGTATAAGATTAAGTTATAATTACTTGAAAGATGGGGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACGATAACATCAAAATAGAAGACTTATTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCCAAAACAATGGAAGTAGCCAGAGGAAGATTGGTTACAGTGGTCAAGTACCTGAAAAATTCTAGCTTGCTTTTGGATAGTGAGGAAGAAGGATGTGTTAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCCGAATCTGAAGATTGCCATCGGTTTTTGGTGGAAGCTGGTCGTGGTTTAAAGGATTGGGCGCCATGGGGATTACATGAAGGCTGCACTGCAATTTCACTGATGAACAACAAAATTCGCAAGCTACCCAAAGAGATGGTATGTCCAAATCTCCAGATTTTATTACTAAACGGGAATAAGAAACTAAGTGAGATCCCCCAAAAGTTGATCCAGAATCTGAAAAAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAGATGATTGCAGCAAATTAATTGACATTTCCGTTGTGGGGAAACTTAACAAGCTTGAAATTCTTAGTATGAGAGGTTGTCCCAGGGGGCAATTGTTCAGAGAAATAGAACATTTGACCAATCTAAGGATTTTGGATGTCAATCGCTCTTGGGCAACGGGTGGTATGGTTACAATTCCATCTAAAGTGATACCAATGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTTGAGGAGGACGTGTGGTGTGTAGCTGAGGGACGAAGAGAAAGAACCAATATTATTAGGTTTGATGAGTTAGTTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCTTATCTGATGAAAGTTTCATCCCTAAAAATGTTGAGGCCGCACCAGATTGGAATTACTTTTGTATAACTATCTTCGGCAGATGCACCAGCTCTATATACCGCCCCCTCGCTGTATACGAACATGGAGATCGTAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCAAAGCGGTGACGGAGAAAACAGAGAAGCTTGAGTACAACCGCTGCGGAGGGATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTTGCTGGGCAACAAGACGAGTTGAAAGAGTTGATGAACACAACAAGACGAGTTGAAACAGGACCCGTGTTTGAGAATTTGGAAGAGTTGCATCTGATAGATCTGATCCACCTGGAGGAGTTGTTGGTTGGTGAGTTACCACTTGGGTCTCTCTCAAATCTCAAGGTGTTTCATGTATTTTTTGGTAAGATTTTAAGGAGTGTCTCAGAATTTGTACAGAGACTACCAAATCTGGAGAAACTACATTTAAACGGTGTGCATGAATTGGAATATGTGTTTAGATGCGAAGGGTTCGAGCCAGAACAACCAAAACTGAGAGAGATGCATTTGCTGCGTCTAAGAGCACTAAAAAGCGTATGTAATGGTCCTGCTCCACATGCAATGTTCCAGGGTCTTAAAATTTTGACCATTTACCGTTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCCCAATGTCTTGTTCAATTGGAAGACCTTCTTGTTGAGGATTGCCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAACTTGGTTTTGAAGAATCTTCCTAGGTTGTATGGTGCAGTTGCTGCCGTTGATATTCAGTGTCGTTCATTGGAACACTTGATGGTGGAGGATTGCCCCAAGCTTTTATTTTCAACCTCTTCCGATCTCTTTGAAGCCTTCAAAAACAGGATCCAATTTTCATTTTCAGCCTCCATTTCTGACCCAGTCCAACTCGATGATCCACAACTATACCAAACTCTACGTGACAGAACATTGCAAAGTATCATGCTCAGCTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LRR_8 NB-ARC TIR

Protein Analysis

1112

Amino Acids

126.5

Weight (kDa)

6.63

Isoelectric Point (pI)

38.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	14 - 178	1e-46	TIR domain
TIR_2	PF13676	17 - 111	1.6e-16	TIR domain
NB-ARC	PF00931	196 - 360	6.1e-30	NB-ARC domain
LRR_8	PF13855	568 - 626	4.9e-11	Leucine rich repeat
LRR_14	PF23598	571 - 705	3.7e-16	Leucine-rich repeat region
LRR_RPS2	PF23247	865 - 1004	4.4e-18	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0000379)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica	MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis	RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata	RLG00000002871
rosa_multiflora	Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii	Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa	Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana	Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1296
Acc16I	TGCGCA	1 cut(s)	599
Acc36I	ACCTGC	1 cut(s)	2194
AccB1I	GGYRCC	1 cut(s)	1618
AccI	GTMKAC	2 cut(s)	1150, 2325
AccII	CGCG	1 cut(s)	1394
AciI	CCGC	7 cut(s)	180, 660, 2250, 2311, 2408, 2443, 2448
AclI	AACGTT	1 cut(s)	134
AcoI	YGGCCR	1 cut(s)	447
AcsI	RAATTY	9 cut(s)	303, 806, 1477, 1671, 1926, 2188, 2590, 2723, 2913
AcuI	CTGAAG	2 cut(s)	1587, 3092
AcyI	GRCGYC	1 cut(s)	1619
AfiI	CCNNNNNNNGG	5 cut(s)	763, 1905, 1992, 2629, 3019
AflII	CTTAAG	1 cut(s)	436
AflIII	ACRYGT	2 cut(s)	688, 2115
AhlI	ACTAGT	1 cut(s)	1808
AjiI	CACGTC	1 cut(s)	2116
AjnI	CCWGG	5 cut(s)	980, 1197, 1949, 2625, 2901
AjuI	GAANNNNNNNTTGG	4 cut(s)	1313, 1345, 2964, 2996
Alw21I	GWGCWC	1 cut(s)	2860
Alw26I	GTCTC	5 cut(s)	679, 1844, 2667, 2722, 2728
AlwNI	CAGNNNCTG	5 cut(s)	827, 1774, 2519, 3119, 3333
AoxI	GGCC	4 cut(s)	3, 447, 1027, 2247
ApoI	RAATTY	9 cut(s)	303, 806, 1477, 1671, 1926, 2188, 2590, 2723, 2913
AseI	ATTAAT	1 cut(s)	1886
Asp700I	GAANNNNTTC	4 cut(s)	359, 771, 1087, 2994
AspA2I	CCTAGG	1 cut(s)	3098
AspLEI	GCGC	3 cut(s)	600, 616, 1621
AspS9I	GGNCC	4 cut(s)	1059, 1195, 2576, 2879
AsuC2I	CCSGG	1 cut(s)	1538
AsuHPI	GGTGA	5 cut(s)	694, 967, 1837, 2422, 2657
AvaII	GGWCC	4 cut(s)	1059, 1195, 2576, 2879
AvrII	CCTAGG	1 cut(s)	3098
BaeGI	GKGCMC	1 cut(s)	1183
BaeI	ACNNNNGTAYC	2 cut(s)	2048, 2081
BalI	TGGCCA	1 cut(s)	449
BamHI	GGATCC	1 cut(s)	3225
BanI	GGYRCC	1 cut(s)	1618
BanII	GRGCYC	1 cut(s)	1101
BarI	GAAGNNNNNNTAC	4 cut(s)	1438, 1470, 2420, 2452
BbsI	GAAGAC	4 cut(s)	1362, 1383, 2997, 3059
Bbv12I	GWGCWC	1 cut(s)	2860
BbvCI	CCTCAGC	1 cut(s)	2130
BccI	CCATC	6 cut(s)	1304, 1584, 1687, 2053, 2384, 3151
BceAI	ACGGC	1 cut(s)	3107
BciT130I	CCWGG	5 cut(s)	982, 1199, 1951, 2627, 2903
BciVI	GTATCC	1 cut(s)	1534
BcnI	CCSGG	1 cut(s)	1538
BcoDI	GTCTC	5 cut(s)	679, 1844, 2667, 2722, 2728
BcuI	ACTAGT	1 cut(s)	1808
BfaI	CTAG	4 cut(s)	1229, 1482, 1809, 3099
BfmI	CTRYAG	1 cut(s)	706
BfoI	RGCGCY	2 cut(s)	617, 1622
BfrI	CTTAAG	1 cut(s)	436
BfuAI	ACCTGC	1 cut(s)	2194
BfuI	GTATCC	1 cut(s)	1534
BglII	AGATCT	2 cut(s)	99, 2614
BlnI	CCTAGG	1 cut(s)	3098
BlpI	GCTNAGC	1 cut(s)	3326
Bme1390I	CCNGG	6 cut(s)	982, 1199, 1538, 1951, 2627, 2903
Bme18I	GGWCC	4 cut(s)	1059, 1195, 2576, 2879
BmgBI	CACGTC	1 cut(s)	2116
BmgT120I	GGNCC	4 cut(s)	1059, 1195, 2576, 2879
BmiI	GGNNCC	3 cut(s)	1620, 2578, 3227
BmrFI	CCNGG	6 cut(s)	982, 1199, 1538, 1951, 2627, 2903
BmrI	ACTGGG	1 cut(s)	3254
BmuI	ACTGGG	1 cut(s)	3254
BpiI	GAAGAC	4 cut(s)	1362, 1383, 2997, 3059
BplI	GAGNNNNNCTC	2 cut(s)	1088, 1120
BpmI	CTGGAG	3 cut(s)	1694, 2648, 2768
Bpu10I	CCTNAGC	1 cut(s)	2130
Bpu1102I	GCTNAGC	1 cut(s)	3326
BpuEI	CTTGAG	3 cut(s)	2453, 2486, 2660
BpuMI	CCSGG	1 cut(s)	1538
BsaAI	YACGTR	1 cut(s)	3299
BsaBI	GATNNNNATC	2 cut(s)	1575, 2007
BsaHI	GRCGYC	1 cut(s)	1619
BsaI	GGTCTC	1 cut(s)	2667
BsaJI	CCNNGG	7 cut(s)	288, 1622, 1949, 1950, 2482, 2902, 3098
BsaWI	WCCGGW	1 cut(s)	1386
BsaXI	ACNNNNNCTCC	4 cut(s)	2102, 2132, 2621, 2651
Bsc4I	CCNNNNNNNGG	5 cut(s)	763, 1905, 1992, 2629, 3019
Bse1I	ACTGG	1 cut(s)	3260
Bse3DI	GCAATG	2 cut(s)	2901, 3308
Bse8I	GATNNNNATC	2 cut(s)	1575, 2007
BseBI	CCWGG	5 cut(s)	982, 1199, 1951, 2627, 2903
BseDI	CCNNGG	7 cut(s)	288, 1622, 1949, 1950, 2482, 2902, 3098
BseGI	GGATG	8 cut(s)	148, 172, 343, 1517, 1533, 2008, 2229, 2460
BseJI	GATNNNNATC	2 cut(s)	1575, 2007
BseLI	CCNNNNNNNGG	5 cut(s)	763, 1905, 1992, 2629, 3019
BseMI	GCAATG	2 cut(s)	2901, 3308
BseMII	CTCAG	6 cut(s)	471, 538, 1056, 2121, 2733, 2819
BseNI	ACTGG	1 cut(s)	3260
BseRI	GAGGAG	4 cut(s)	512, 1832, 2123, 2645
BseSI	GKGCMC	1 cut(s)	1183
BseYI	CCCAGC	1 cut(s)	2519
BsgI	GTGCAG	3 cut(s)	681, 1627, 3132
Bsh1236I	CGCG	1 cut(s)	1394
Bsh1285I	CGRYCG	1 cut(s)	1398
BshFI	GGCC	4 cut(s)	5, 449, 1029, 2249
BshNI	GGYRCC	1 cut(s)	1618
BsiEI	CGRYCG	1 cut(s)	1398
BsiHKAI	GWGCWC	1 cut(s)	2860
BsiSI	CCGG	2 cut(s)	1387, 1537
BslFI	GGGAC	3 cut(s)	832, 1932, 2148
BslI	CCNNNNNNNGG	5 cut(s)	763, 1905, 1992, 2629, 3019
BsmAI	GTCTC	5 cut(s)	679, 1844, 2667, 2722, 2728
BsmFI	GGGAC	3 cut(s)	832, 1932, 2148
BsmI	GAATGC	1 cut(s)	1271
BsnI	GGCC	4 cut(s)	5, 449, 1029, 2249
Bso31I	GGTCTC	1 cut(s)	2667
Bsp1286I	GDGCHC	3 cut(s)	1101, 1183, 2860
Bsp1407I	TGTACA	2 cut(s)	2085, 2728
Bsp1720I	GCTNAGC	1 cut(s)	3326
Bsp19I	CCATGG	3 cut(s)	288, 1622, 2482
BspACI	CCGC	7 cut(s)	180, 660, 2250, 2311, 2408, 2443, 2448
BspANI	GGCC	4 cut(s)	5, 449, 1029, 2249
BspCNI	CTCAG	6 cut(s)	470, 537, 1055, 2122, 2732, 2820
BspFNI	CGCG	1 cut(s)	1394
BspHI	TCATGA	1 cut(s)	858
BspLI	GGNNCC	3 cut(s)	1620, 2578, 3227
BspMAI	CTGCAG	1 cut(s)	710
BspMI	ACCTGC	1 cut(s)	2194
BspQI	GCTCTTC	1 cut(s)	826
BspT107I	GGYRCC	1 cut(s)	1618
BspTI	CTTAAG	1 cut(s)	436
BspTNI	GGTCTC	1 cut(s)	2667
BsrDI	GCAATG	2 cut(s)	2901, 3308
BsrGI	TGTACA	2 cut(s)	2085, 2728
BsrI	ACTGG	1 cut(s)	3260
BssECI	CCNNGG	7 cut(s)	288, 1622, 1949, 1950, 2482, 2902, 3098
BssNAI	GTATAC	1 cut(s)	2326
BssNI	GRCGYC	1 cut(s)	1619
BssT1I	CCWWGG	4 cut(s)	288, 1622, 2482, 3098
Bst1107I	GTATAC	1 cut(s)	2326
Bst2UI	CCWGG	5 cut(s)	982, 1199, 1951, 2627, 2903
Bst4CI	ACNGT	9 cut(s)	359, 491, 637, 1459, 2099, 2515, 2768, 2930, 3058
Bst6I	CTCTTC	6 cut(s)	19, 288, 826, 2134, 2592, 3199
BstACI	GRCGYC	1 cut(s)	1619
BstAFI	CTTAAG	1 cut(s)	436
BstAPI	GCANNNNNTGC	2 cut(s)	2845, 3119
BstAUI	TGTACA	2 cut(s)	2085, 2728
BstBAI	YACGTR	1 cut(s)	3299
BstC8I	GCNNGC	4 cut(s)	706, 1487, 1679, 2936
BstDSI	CCRYGG	3 cut(s)	288, 1622, 2482
BstF5I	GGATG	8 cut(s)	148, 172, 343, 1517, 1533, 2008, 2229, 2460
BstFNI	CGCG	1 cut(s)	1394
BstH2I	RGCGCY	2 cut(s)	617, 1622
BstHHI	GCGC	3 cut(s)	600, 616, 1621
BstMAI	GTCTC	5 cut(s)	679, 1844, 2667, 2722, 2728
BstMCI	CGRYCG	1 cut(s)	1398
BstMWI	GCNNNNNNNGC	8 cut(s)	248, 660, 701, 833, 1166, 2845, 2891, 3119
BstNI	CCWGG	5 cut(s)	982, 1199, 1951, 2627, 2903
BstNSI	RCATGY	2 cut(s)	692, 2894
BstSCI	CCNGG	6 cut(s)	980, 1197, 1536, 1949, 2625, 2901
BstSFI	CTRYAG	1 cut(s)	706
BstSLI	GKGCMC	1 cut(s)	1183
BstUI	CGCG	1 cut(s)	1394
BstV2I	GAAGAC	4 cut(s)	1362, 1383, 2997, 3059
BstX2I	RGATCY	6 cut(s)	99, 1749, 1795, 2351, 2614, 3225
BstXI	CCANNNNNNTGG	2 cut(s)	1429, 1693
BstYI	RGATCY	6 cut(s)	99, 1749, 1795, 2351, 2614, 3225
BstZ17I	GTATAC	1 cut(s)	2326
BsuI	GTATCC	1 cut(s)	1534
BsuRI	GGCC	4 cut(s)	5, 449, 1029, 2249
BtgI	CCRYGG	3 cut(s)	288, 1622, 2482
BtrI	CACGTC	1 cut(s)	2116
BtsCI	GGATG	8 cut(s)	148, 172, 343, 1517, 1533, 2008, 2229, 2460
BtsI	GCAGTG	2 cut(s)	1644, 3045
BtsIMutI	CAGTG	6 cut(s)	496, 1464, 1644, 1655, 3045, 3140
BveI	ACCTGC	1 cut(s)	2194
Cac8I	GCNNGC	4 cut(s)	706, 1487, 1679, 2936
CaiI	CAGNNNCTG	5 cut(s)	827, 1774, 2519, 3119, 3333
CciI	TCATGA	1 cut(s)	858
CfoI	GCGC	3 cut(s)	600, 616, 1621
Cfr13I	GGNCC	4 cut(s)	1059, 1195, 2576, 2879
CseI	GACGC	1 cut(s)	2837
CspCI	CAANNNNNGTGG	2 cut(s)	1090, 1125
DinI	GGCGCC	1 cut(s)	1620
DraI	TTTAAA	3 cut(s)	805, 1608, 2763
EaeI	YGGCCR	1 cut(s)	447
Eam1104I	CTCTTC	6 cut(s)	19, 288, 826, 2134, 2592, 3199
EarI	CTCTTC	6 cut(s)	19, 288, 826, 2134, 2592, 3199
Eco130I	CCWWGG	4 cut(s)	288, 1622, 2482, 3098
Eco24I	GRGCYC	1 cut(s)	1101
Eco31I	GGTCTC	1 cut(s)	2667
Eco47I	GGWCC	4 cut(s)	1059, 1195, 2576, 2879
Eco57I	CTGAAG	2 cut(s)	1587, 3092
EcoO109I	RGGNCCY	1 cut(s)	2576
EcoRII	CCWGG	5 cut(s)	980, 1197, 1949, 2625, 2901
EcoT14I	CCWWGG	4 cut(s)	288, 1622, 2482, 3098
EcoT22I	ATGCAT	3 cut(s)	1273, 1527, 2841
EcoT38I	GRGCYC	1 cut(s)	1101
EgeI	GGCGCC	1 cut(s)	1620
EheI	GGCGCC	1 cut(s)	1620
ErhI	CCWWGG	4 cut(s)	288, 1622, 2482, 3098
FalI	AAGNNNNNCTT	6 cut(s)	522, 554, 1313, 1345, 2982, 3014
FaqI	GGGAC	3 cut(s)	832, 1932, 2148
FauI	CCCGC	1 cut(s)	187
FblI	GTMKAC	2 cut(s)	1150, 2325
FokI	GGATG	8 cut(s)	155, 179, 350, 1520, 1524, 2015, 2216, 2467
FriOI	GRGCYC	1 cut(s)	1101
FspBI	CTAG	4 cut(s)	1229, 1482, 1809, 3099
FspI	TGCGCA	1 cut(s)	599
GlaI	GCGC	3 cut(s)	599, 615, 1620
GsaI	CCCAGC	1 cut(s)	2523
GsuI	CTGGAG	3 cut(s)	1694, 2648, 2768
HaeII	RGCGCY	2 cut(s)	617, 1622
HaeIII	GGCC	4 cut(s)	5, 449, 1029, 2249
HapII	CCGG	2 cut(s)	1387, 1537
HgaI	GACGC	1 cut(s)	2837
HhaI	GCGC	3 cut(s)	600, 616, 1621
Hin1I	GRCGYC	1 cut(s)	1619
Hin6I	GCGC	3 cut(s)	598, 614, 1619
HinP1I	GCGC	3 cut(s)	598, 614, 1619
HincII	GTYRAC	1 cut(s)	502
HindII	GTYRAC	1 cut(s)	502
HindIII	AAGCTT	5 cut(s)	369, 1857, 1919, 2429, 3176
HpaII	CCGG	2 cut(s)	1387, 1537
HphI	GGTGA	5 cut(s)	694, 967, 1837, 2422, 2657
Hpy166II	GTNNAC	7 cut(s)	494, 502, 889, 1151, 2095, 2326, 3043
Hpy8I	GTNNAC	7 cut(s)	494, 502, 889, 1151, 2095, 2326, 3043
Hpy99I	CGWCG	1 cut(s)	28
HpyAV	CCTTC	7 cut(s)	406, 1207, 1502, 1631, 2795, 3005, 3223
HpyCH4III	ACNGT	9 cut(s)	359, 491, 637, 1459, 2099, 2515, 2768, 2930, 3058
HpyCH4IV	ACGT	3 cut(s)	134, 2115, 3298
HpyF10VI	GCNNNNNNNGC	8 cut(s)	248, 660, 701, 833, 1166, 2845, 2891, 3119
HpySE526I	ACGT	3 cut(s)	134, 2115, 3298
Hsp92I	GRCGYC	1 cut(s)	1619
HspAI	GCGC	3 cut(s)	598, 614, 1619
KasI	GGCGCC	1 cut(s)	1618
LguI	GCTCTTC	1 cut(s)	826
LmnI	GCTCC	2 cut(s)	2369, 2890
MaeI	CTAG	4 cut(s)	1229, 1482, 1809, 3099
MaeII	ACGT	3 cut(s)	134, 2115, 3298
MaeIII	GTNAC	7 cut(s)	1453, 2035, 2410, 2459, 2490, 2649, 3299
MfeI	CAATTG	2 cut(s)	1958, 2984
MflI	RGATCY	6 cut(s)	99, 1749, 1795, 2351, 2614, 3225
MhlI	GDGCHC	3 cut(s)	1101, 1183, 2860
MlsI	TGGCCA	1 cut(s)	449
MluNI	TGGCCA	1 cut(s)	449
Mly113I	GGCGCC	1 cut(s)	1619
MlyI	GAGTC	2 cut(s)	212, 478
MmeI	TCCRAC	3 cut(s)	646, 3000, 3289
Mox20I	TGGCCA	1 cut(s)	449
Mph1103I	ATGCAT	3 cut(s)	1273, 1527, 2841
MroXI	GAANNNNTTC	4 cut(s)	359, 771, 1087, 2994
MscI	TGGCCA	1 cut(s)	449
MslI	CAYNNNNRTG	2 cut(s)	1276, 2468
Msp20I	TGGCCA	1 cut(s)	449
MspA1I	CMGCKG	2 cut(s)	36, 2445
MspCI	CTTAAG	1 cut(s)	436
MspI	CCGG	2 cut(s)	1387, 1537
MspR9I	CCNGG	6 cut(s)	982, 1199, 1538, 1951, 2627, 2903
MunI	CAATTG	2 cut(s)	1958, 2984
Mva1269I	GAATGC	1 cut(s)	1271
MvaI	CCWGG	5 cut(s)	982, 1199, 1951, 2627, 2903
MvnI	CGCG	1 cut(s)	1394
MwoI	GCNNNNNNNGC	8 cut(s)	248, 660, 701, 833, 1166, 2845, 2891, 3119
NarI	GGCGCC	1 cut(s)	1619
NciI	CCSGG	1 cut(s)	1538
NcoI	CCATGG	3 cut(s)	288, 1622, 2482
NlaIV	GGNNCC	3 cut(s)	1620, 2578, 3227
NmuCI	GTSAC	3 cut(s)	2410, 2459, 3299
NsbI	TGCGCA	1 cut(s)	599
NsiI	ATGCAT	3 cut(s)	1273, 1527, 2841
NspI	RCATGY	2 cut(s)	692, 2894
PagI	TCATGA	1 cut(s)	858
PasI	CCCWGGG	1 cut(s)	1950
PciI	ACATGT	1 cut(s)	688
PciSI	GCTCTTC	1 cut(s)	826
PcsI	WCGNNNNNNNCGW	1 cut(s)	2325
PctI	GAATGC	1 cut(s)	1271
PdmI	GAANNNNTTC	4 cut(s)	359, 771, 1087, 2994
PflFI	GACNNNGTC	1 cut(s)	3260
PfoI	TCCNGGA	1 cut(s)	1536
Ple19I	CGATCG	1 cut(s)	1398
PleI	GAGTC	2 cut(s)	211, 478
PluTI	GGCGCC	1 cut(s)	1622
PpsI	GAGTC	2 cut(s)	211, 478
Ppu21I	YACGTR	1 cut(s)	3299
PpuMI	RGGWCCY	1 cut(s)	2576
PscI	ACATGT	1 cut(s)	688
PshBI	ATTAAT	1 cut(s)	1886
PsiI	TTATAA	1 cut(s)	1296
Psp1406I	AACGTT	1 cut(s)	134
Psp5II	RGGWCCY	1 cut(s)	2576
Psp6I	CCWGG	5 cut(s)	980, 1197, 1949, 2625, 2901
PspFI	CCCAGC	1 cut(s)	2519
PspGI	CCWGG	5 cut(s)	980, 1197, 1949, 2625, 2901
PspN4I	GGNNCC	3 cut(s)	1620, 2578, 3227
PspPI	GGNCC	4 cut(s)	1059, 1195, 2576, 2879
PspPPI	RGGWCCY	1 cut(s)	2576
PstI	CTGCAG	1 cut(s)	710
PstNI	CAGNNNCTG	5 cut(s)	827, 1774, 2519, 3119, 3333
PsuI	RGATCY	6 cut(s)	99, 1749, 1795, 2351, 2614, 3225
PsyI	GACNNNGTC	1 cut(s)	3260
PvuI	CGATCG	1 cut(s)	1398
PvuII	CAGCTG	1 cut(s)	36
RseI	CAYNNNNRTG	2 cut(s)	1276, 2468
SapI	GCTCTTC	1 cut(s)	826
Sau96I	GGNCC	4 cut(s)	1059, 1195, 2576, 2879
SchI	GAGTC	2 cut(s)	212, 478
ScrFI	CCNGG	6 cut(s)	982, 1199, 1538, 1951, 2627, 2903
SduI	GDGCHC	3 cut(s)	1101, 1183, 2860
SfcI	CTRYAG	1 cut(s)	706
SfoI	GGCGCC	1 cut(s)	1620
SinI	GGWCC	4 cut(s)	1059, 1195, 2576, 2879
SmiMI	CAYNNNNRTG	2 cut(s)	1276, 2468
SmlI	CTYRAG	4 cut(s)	436, 2432, 2501, 2675
SmoI	CTYRAG	4 cut(s)	436, 2432, 2501, 2675
SpeI	ACTAGT	1 cut(s)	1808
SsiI	CCGC	7 cut(s)	180, 660, 2250, 2311, 2408, 2443, 2448
SspDI	GGCGCC	1 cut(s)	1618
SspI	AATATT	2 cut(s)	2155, 2199
SspMI	CTAG	4 cut(s)	1229, 1482, 1809, 3099
StyD4I	CCNGG	6 cut(s)	980, 1197, 1536, 1949, 2625, 2901
StyI	CCWWGG	4 cut(s)	288, 1622, 2482, 3098
TaaI	ACNGT	9 cut(s)	359, 491, 637, 1459, 2099, 2515, 2768, 2930, 3058
TaiI	ACGT	3 cut(s)	137, 2118, 3301
TaqI	TCGA	6 cut(s)	26, 109, 230, 1414, 2808, 3270
TatI	WGTACW	4 cut(s)	2085, 2094, 2436, 2728
TauI	GCSGC	2 cut(s)	663, 2252
TscAI	CASTG	6 cut(s)	496, 1464, 1651, 1662, 3045, 3140
TseFI	GTSAC	3 cut(s)	2410, 2459, 3299
Tsp45I	GTSAC	3 cut(s)	2410, 2459, 3299
TspGWI	ACGGA	4 cut(s)	350, 1807, 1888, 2429
TspRI	CASTG	6 cut(s)	496, 1464, 1651, 1662, 3045, 3140
Tth111I	GACNNNGTC	1 cut(s)	3260
Vha464I	CTTAAG	1 cut(s)	436
VpaK11BI	GGWCC	4 cut(s)	1059, 1195, 2576, 2879
VspI	ATTAAT	1 cut(s)	1886
XapI	RAATTY	9 cut(s)	303, 806, 1477, 1671, 1926, 2188, 2590, 2723, 2913
XceI	RCATGY	2 cut(s)	692, 2894
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1447
XmaJI	CCTAGG	1 cut(s)	3098
XmiI	GTMKAC	2 cut(s)	1150, 2325
XmnI	GAANNNNTTC	4 cut(s)	359, 771, 1087, 2994
XspI	CTAG	4 cut(s)	1229, 1482, 1809, 3099
Zsp2I	ATGCAT	3 cut(s)	1273, 1527, 2841

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.