Rroxscaffold_3G00228660

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000379
Physical Location & Seq
Forward (+)
13079372 .. 13089961
10590 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00228660.1

Sequence Viewer

Length: 3363 bp
ATGGTTCAGAAGCATGAGCCAATATGGCCGGATAATCAAATAATGGTCGAGGCCAATGACTTTGCATCTCCTCAGTGGAAGCATGATGTGTTTTTAAGTTTCAGGGGTCCTGACACTCGCAAGGGTATCACAGTTGAACTATACGATCGACTGCAGATGAGGGGAATTAAAACATTCATGGATGATAGAGATCTTGAAGTAGGGGATGCTATTTCTCGCTCTCTCATAGCAGCCATTGAAAAATCAAGGTTTGCAATCATTGTTCTCTCACGAGACTACGCTTCTTCTACTTGGTGTTTGGAGGAACTTACAAAGATCTGCGAGTGCATGAAAGACCAGAACAGAATTTTTCCACTTTTTTTTCATGTGGAGCCCTCTGATGTACGTCATCAGAAGAGGAGTTTGGATGAGGCTTTCTCTAAACATGAAACCTCCGGGCGACATGGATTGGAGAAGATGCGGCGGTGGAGGGATGCTTTAGAAAAGGTGGCCAGTTTCTCTGGCTGGAATACACAGGATTATAAGACTCACAAAGAACTTGTTGACGTCATTGTGGAATTTCTCCGCAATAAAGTGCGACCTGATGCAATTGAGTTCAGTGGAGATTTCGAAGCATATGAAGCAACAAGAAAGGCCATGGATGAGGTTATGAAGGCACTAAAAGATGACAAGGTCACTGCTGTTGGGGTCTACGGCATGGGAGGTGTTGGAAAGACATGCATGGTGAAACATGTCGGTGCACAAGGCTGCAAAAATGGTAACTTTAACCATAAGATTATGGCTGCCATATCTCAAAGCCCTGACTTGAGAAAAATTCAAGGCACATTGGCTGATCTGTTGGGCGTTAAATTGGAGGAGGAGACAGAAATTGGAAGAGCCGCTACGTTGCATAAGGAGATGATGAGAAGAGAAAAACTCCTTATAATCTTGGACGATATTTGGGAGAGAATAGAGTTGTCAAAGATAGGGATTCCCAGCTACAAGGAGCTTCAAATGTGCAAGTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCATGTCATGAGATGCCAGAGAAAGATCACCCTCAGTATCCTATCAAAAGAGGATTCTTGGGCCTTGTTTGTGAGAAATGTAGGAATGCCTTTTGAATCCACCAAATTTAAGCATGTAGCTAAGAAGGTGGCTGGAGAATGCAGGGGTTTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGAGATAAAGACCTGGCTGAATGGGAAAAAGCAGCTCAACGACTAGAGAAGTCACAGTTTGCGAACCCTGACCATCAGGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAAGATGAGGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGACTATGACATCCGACTAGAAAACTTATTCAAGTATGCAATCGGGAAAGGATTGTTTCGAGATGCTGAAACAATCGAAGAAGCCAGAGGAACAGCAGATTCTGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGTGAAGAAGATGGATGTGTAAGGATGCATGATGTCATCCGGGATACAGCCATGAAGATTGCAAAATCTGAGGATGGACATGGGTTTTTGGTAAAAGCTGGGTCTGGTTTGAAGGATTGGTCATGTCCATTACATGAAAGCTATTCTGCAATTTCACTGATGAGGAACGAAATTCGCAAGCTACCTGAAGAGTTAGTATGTCCAAATCTCCAGATTTTATTACTAAAACGGAATGCTGATTTAAATGAGATCCCAGAAAAATTTATCCAAAGTCTGAATGAATTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCTGTACTACCCCAATCATTCAGTCTCCTCATCAACCTCCAAGCTTTGTTTTTAGATTTTTGCCACAACATGATTGACATTTCCATAGTTGGAAAACTTAAGAAGCTTGAAATTCTTAGTATGAGAGAATATCCTCTCCAGGAATTATCAAGAGAAATAGGACACTTGACCAATCTAAGGATTTTGGATGTCAATTCCGCTGGTTATGAGGGTGGAGGTATTGTCACAATTCCATCAAAAGTGATATCAAAGTTGCATAGATTAGAAGAACTGTATACACTAAACTGTGGATTTGAGGATTGGGGGAGTAAAGTTGAGAGAGAAGGAGGGGAAATCGAATTGGGTTTCACTGGTTTGTCAAATTTGAAAATTTTGCAGGTTTGCATATCTGATGCAAAGTGCATCCCTACAAGAATTGAGGTCGAACCAGAGTGGGTTTACTTTCATATACTTATCGGTAAATACATAAGGTATCCATACAATCGATATCATCATAACTCAAGATCCTTGGTTCTTGACACTACCATGAGTACCTTACCTGATTGGTTTATCAATGTGGTGACAACAAAAACAGAGACGCTAAAGTATGATCGCTGCAAAGGGTTGAGTGACATTGTCGTGGAATATGACCATGGGAGGTTGCATGAACTCAAGCATCTCACTGTATCTGATAATATACTTGACCCCTATGAGAACATGAAAGAGTTGATGAACACAACGAGACGGGTTCAAAAAGGAGCAGTGTTTGAGAACTTGGAAGAGTTGCATCTGAAATGTCTGCTCAAACTTGAAGAGTTGTGTGTTGGTCAGTTACCACCTGGGTCTCTCATCAATCTTAAGTTATTGCATGTGTTTTTTTGTAATAACTTGGTGAATGTATCAAAATTGTTACAAAGACTGCCAAATCTGGAGAAACTATATTTAAATTGTATGGATGAAATGGAATTTGTGTTTGGATCTGAAGGGTTTGAACCAGAAGAGTCAAAACTGAGAGAGATAAATTTGTTGGATCTACATGCACTGAGAAGCATATGTAATGCTTCCCCACGTTTAATGTTGCAGAGTCTTAAAAGTTTGACCATATACCGTTGTAAGTTACTGCAAAGTCTGTTTGCATCTGATGTAGCCGAGTGTCTTTTTCAATTGGAAGACCTTTTTGTGGAGCAGTGCCCTTTGTTGGAAAGAGTAATTGAAGCAGTAAACAAGAAGAAGAAGGTTCTTCCAAAATTGAAGAACTTGGTTTTGAAGAAACTTCATATGTTGTATGGTGCAACTGATCCTGTTGATATTGAGTGTCCTTCATTGGAACAGTTGATTGTGGTGGATTGCCCCCAGATTCCATTTTCAACCTCTTCCTCTCTCTTCTTTGCCTTCAAAAGCATGTTCCAGTTTTCAATTTCAACCTCTGCTTCTGACTACTTTTGCAGCAAGAACCCAGTACAACTCAATGATCGACAACTGTACACCTTTCTACGTCACAGATGCAGCTGTGTAACTCCTTTCCAACCGTAA

Protein Analysis

1120

Amino Acids

128.35

Weight (kDa)

6.43

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 192 2.2e-44 TIR domain
TIR_2 PF13676 30 - 126 1e-16 TIR domain
NB-ARC PF00931 211 - 375 3.2e-29 NB-ARC domain
LRR_8 PF13855 580 - 639 3.7e-08 Leucine rich repeat
LRR_14 PF23598 585 - 714 9.1e-15 Leucine-rich repeat region
LRR_RPS2 PF23247 869 - 1007 1.7e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 522, 923
AatII GACGTC 1 cut(s) 549
Acc36I ACCTGC 1 cut(s) 2218
AccI GTMKAC 2 cut(s) 690, 2126
AciI CCGC 5 cut(s) 460, 463, 565, 879, 2049
AclWI GGATC 6 cut(s) 1780, 1839, 2349, 2815, 2868, 3122
AcoI YGGCCR 2 cut(s) 26, 489
AcuI CTGAAG 2 cut(s) 1743, 2832
AcyI GRCGYC 1 cut(s) 546
AfaI GTAC 6 cut(s) 384, 1508, 1857, 2383, 3291, 3314
AfiI CCNNNNNNNGG 3 cut(s) 2028, 3010, 3028
AflII CTTAAG 2 cut(s) 1949, 2687
AflIII ACRYGT 1 cut(s) 730
AhdI GACNNNNNGTC 1 cut(s) 1360
AhlI ACTAGT 1 cut(s) 1844
AjnI CCWGG 3 cut(s) 1236, 1989, 2668
AjuI GAANNNNNNNTTGG 4 cut(s) 386, 418, 2787, 2819
Alw21I GWGCWC 1 cut(s) 742
Alw26I GTCTC 6 cut(s) 267, 854, 1880, 2420, 2566, 2679
Alw44I GTGCAC 1 cut(s) 738
AlwI GGATC 6 cut(s) 1780, 1839, 2349, 2815, 2868, 3122
AlwNI CAGNNNCTG 1 cut(s) 1496
AoxI GGCC 5 cut(s) 26, 51, 489, 633, 1101
ApaLI GTGCAC 1 cut(s) 738
ApeKI GCWGC 7 cut(s) 230, 747, 782, 1256, 2445, 3276, 3336
AspS9I GGNCC 3 cut(s) 107, 1101, 1354
AsuC2I CCSGG 2 cut(s) 436, 1577
AsuHPI GGTGA 5 cut(s) 736, 1009, 1060, 2422, 2734
AsuII TTCGAA 1 cut(s) 609
AvaII GGWCC 2 cut(s) 107, 1354
BaeGI GKGCMC 3 cut(s) 742, 1222, 3023
BalI TGGCCA 1 cut(s) 491
BanII GRGCYC 1 cut(s) 375
BarI GAAGNNNNNNTAC 2 cut(s) 865, 897
BauI CACGAG 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 1401, 3006
Bbv12I GWGCWC 1 cut(s) 742
BbvI GCAGC 7 cut(s) 242, 734, 769, 1268, 2432, 3288, 3348
BccI CCATC 4 cut(s) 1305, 1541, 1604, 2092
BceAI ACGGC 1 cut(s) 709
BciT130I CCWGG 3 cut(s) 1238, 1991, 2670
BciVI GTATCC 3 cut(s) 1088, 1573, 2334
BcnI CCSGG 2 cut(s) 436, 1577
BcoDI GTCTC 6 cut(s) 267, 854, 1880, 2420, 2566, 2679
BcuI ACTAGT 1 cut(s) 1844
BfaI CTAG 4 cut(s) 1268, 1412, 1521, 1845
BfmI CTRYAG 1 cut(s) 152
BfrI CTTAAG 2 cut(s) 1949, 2687
BfuAI ACCTGC 1 cut(s) 2218
BfuI GTATCC 3 cut(s) 1088, 1573, 2334
BglI GCCNNNNNGGC 1 cut(s) 25
BglII AGATCT 2 cut(s) 190, 315
BisI GCNGC 9 cut(s) 231, 461, 748, 783, 879, 1257, 2446, 3277, 3337
BlsI GCNGC 9 cut(s) 232, 462, 749, 784, 880, 1258, 2447, 3278, 3338
Bme1390I CCNGG 5 cut(s) 436, 1238, 1577, 1991, 2670
Bme18I GGWCC 2 cut(s) 107, 1354
BmeRI GACNNNNNGTC 1 cut(s) 1360
BmgT120I GGNCC 3 cut(s) 107, 1101, 1354
BmiI GGNNCC 2 cut(s) 108, 372
BmrFI CCNGG 5 cut(s) 436, 1238, 1577, 1991, 2670
BmrI ACTGGG 1 cut(s) 3281
BmuI ACTGGG 1 cut(s) 3281
BpiI GAAGAC 2 cut(s) 1401, 3006
BplI GAGNNNNNCTC 4 cut(s) 401, 433, 900, 932
BpmI CTGGAG 4 cut(s) 1194, 1730, 1973, 2780
Bpu14I TTCGAA 1 cut(s) 609
BpuEI CTTGAG 3 cut(s) 826, 2335, 2486
BpuMI CCSGG 2 cut(s) 436, 1577
Bsa29I ATCGAT 1 cut(s) 2335
BsaBI GATNNNNATC 1 cut(s) 189
BsaHI GRCGYC 1 cut(s) 546
BsaI GGTCTC 1 cut(s) 2679
BsaJI CCNNGG 4 cut(s) 636, 2358, 2482, 2669
BsaXI ACNNNNNCTCC 2 cut(s) 2058, 2088
Bsc4I CCNNNNNNNGG 3 cut(s) 2028, 3010, 3028
Bse1I ACTGG 4 cut(s) 492, 2206, 3238, 3287
Bse8I GATNNNNATC 1 cut(s) 189
BseBI CCWGG 3 cut(s) 1238, 1991, 2670
BseCI ATCGAT 1 cut(s) 2335
BseDI CCNNGG 4 cut(s) 636, 2358, 2482, 2669
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 3 cut(s) 2028, 3010, 3028
BseMII CTCAG 5 cut(s) 86, 1087, 1596, 2831, 2864
BseNI ACTGG 4 cut(s) 492, 2206, 3238, 3287
BseRI GAGGAG 5 cut(s) 60, 412, 869, 872, 1868
BseSI GKGCMC 3 cut(s) 742, 1222, 3023
BseXI GCAGC 7 cut(s) 242, 734, 769, 1268, 2432, 3288, 3348
BseYI CCCAGC 2 cut(s) 974, 1634
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 5 cut(s) 28, 53, 491, 635, 1103
BshVI ATCGAT 1 cut(s) 2335
BsiEI CGRYCG 1 cut(s) 148
BsiHKAI GWGCWC 1 cut(s) 742
BsiSI CCGG 3 cut(s) 29, 435, 1576
BslI CCNNNNNNNGG 3 cut(s) 2028, 3010, 3028
BsmAI GTCTC 6 cut(s) 267, 854, 1880, 2420, 2566, 2679
BsmBI CGTCTC 2 cut(s) 2420, 2566
BsmI GAATGC 4 cut(s) 1131, 1184, 1310, 1774
BsnI GGCC 5 cut(s) 28, 53, 491, 635, 1103
Bso31I GGTCTC 1 cut(s) 2679
Bsp119I TTCGAA 1 cut(s) 609
Bsp1286I GDGCHC 4 cut(s) 375, 742, 1222, 3023
Bsp1407I TGTACA 1 cut(s) 3312
Bsp19I CCATGG 2 cut(s) 636, 2482
BspACI CCGC 5 cut(s) 460, 463, 565, 879, 2049
BspANI GGCC 5 cut(s) 28, 53, 491, 635, 1103
BspCNI CTCAG 5 cut(s) 85, 1086, 1597, 2832, 2865
BspDI ATCGAT 1 cut(s) 2335
BspHI TCATGA 1 cut(s) 1047
BspLI GGNNCC 2 cut(s) 108, 372
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 2218
BspPI GGATC 6 cut(s) 1780, 1839, 2349, 2815, 2868, 3122
BspQI GCTCTTC 1 cut(s) 868
BspT104I TTCGAA 1 cut(s) 609
BspTI CTTAAG 2 cut(s) 1949, 2687
BspTNI GGTCTC 1 cut(s) 2679
BsrGI TGTACA 1 cut(s) 3312
BsrI ACTGG 4 cut(s) 492, 2206, 3238, 3287
BssECI CCNNGG 4 cut(s) 636, 2358, 2482, 2669
BssNAI GTATAC 1 cut(s) 2127
BssNI GRCGYC 1 cut(s) 546
BssSI CACGAG 1 cut(s) 270
BssT1I CCWWGG 3 cut(s) 636, 2358, 2482
Bst1107I GTATAC 1 cut(s) 2127
Bst2BI CACGAG 1 cut(s) 270
Bst2UI CCWGG 3 cut(s) 1238, 1991, 2670
Bst4CI ACNGT 9 cut(s) 133, 1281, 2124, 2138, 2515, 2939, 3162, 3312, 3360
Bst6I CTCTTC 9 cut(s) 389, 868, 901, 1719, 2604, 2637, 2823, 3208, 3218
BstACI GRCGYC 1 cut(s) 546
BstAFI CTTAAG 2 cut(s) 1949, 2687
BstAUI TGTACA 1 cut(s) 3312
BstBI TTCGAA 1 cut(s) 609
BstC8I GCNNGC 2 cut(s) 1526, 1715
BstDEI CTNAG 9 cut(s) 72, 1073, 1161, 1605, 1835, 1967, 2027, 2840, 2873
BstDSI CCRYGG 2 cut(s) 636, 2482
BstMAI GTCTC 6 cut(s) 267, 854, 1880, 2420, 2566, 2679
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 3 cut(s) 25, 620, 1205
BstNI CCWGG 3 cut(s) 1238, 1991, 2670
BstNSI RCATGY 6 cut(s) 720, 734, 1157, 2702, 2870, 3235
BstSCI CCNGG 5 cut(s) 434, 1236, 1575, 1989, 2668
BstSFI CTRYAG 1 cut(s) 152
BstSLI GKGCMC 3 cut(s) 742, 1222, 3023
BstV1I GCAGC 7 cut(s) 242, 734, 769, 1268, 2432, 3288, 3348
BstV2I GAAGAC 2 cut(s) 1401, 3006
BstX2I RGATCY 7 cut(s) 190, 315, 1785, 1831, 2354, 2807, 2860
BstYI RGATCY 7 cut(s) 190, 315, 1785, 1831, 2354, 2807, 2860
BstZ17I GTATAC 1 cut(s) 2127
Bsu15I ATCGAT 1 cut(s) 2335
BsuI GTATCC 3 cut(s) 1088, 1573, 2334
BsuRI GGCC 5 cut(s) 28, 53, 491, 635, 1103
BsuTUI ATCGAT 1 cut(s) 2335
BtgI CCRYGG 2 cut(s) 636, 2482
BtsI GCAGTG 3 cut(s) 675, 2598, 3023
BtsIMutI CAGTG 9 cut(s) 80, 604, 675, 1691, 2199, 2511, 2598, 2870, 3023
BveI ACCTGC 1 cut(s) 2218
Cac8I GCNNGC 2 cut(s) 1526, 1715
CaiI CAGNNNCTG 1 cut(s) 1496
CciI TCATGA 1 cut(s) 1047
Cfr13I GGNCC 3 cut(s) 107, 1101, 1354
ClaI ATCGAT 1 cut(s) 2335
CseI GACGC 1 cut(s) 2437
Csp6I GTAC 6 cut(s) 383, 1507, 1856, 2382, 3290, 3313
CviQI GTAC 6 cut(s) 383, 1507, 1856, 2382, 3290, 3313
DdeI CTNAG 9 cut(s) 72, 1073, 1161, 1605, 1835, 1967, 2027, 2840, 2873
DraI TTTAAA 2 cut(s) 1779, 2775
DriI GACNNNNNGTC 1 cut(s) 1360
EaeI YGGCCR 2 cut(s) 26, 489
Eam1104I CTCTTC 9 cut(s) 389, 868, 901, 1719, 2604, 2637, 2823, 3208, 3218
Eam1105I GACNNNNNGTC 1 cut(s) 1360
EarI CTCTTC 9 cut(s) 389, 868, 901, 1719, 2604, 2637, 2823, 3208, 3218
Eco130I CCWWGG 3 cut(s) 636, 2358, 2482
Eco24I GRGCYC 1 cut(s) 375
Eco31I GGTCTC 1 cut(s) 2679
Eco32I GATATC 2 cut(s) 2097, 2339
Eco47I GGWCC 2 cut(s) 107, 1354
Eco57I CTGAAG 2 cut(s) 1743, 2832
EcoO109I RGGNCCY 1 cut(s) 107
EcoRII CCWGG 3 cut(s) 1236, 1989, 2668
EcoRV GATATC 2 cut(s) 2097, 2339
EcoT14I CCWWGG 3 cut(s) 636, 2358, 2482
EcoT22I ATGCAT 3 cut(s) 722, 1312, 1566
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 3 cut(s) 636, 2358, 2482
Esp3I CGTCTC 2 cut(s) 2420, 2566
FalI AAGNNNNNCTT 2 cut(s) 1352, 1384
FauNDI CATATG 3 cut(s) 616, 2882, 3108
FblI GTMKAC 2 cut(s) 690, 2126
Fnu4HI GCNGC 9 cut(s) 231, 461, 748, 783, 879, 1257, 2446, 3277, 3337
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 9 cut(s) 231, 461, 748, 783, 879, 1257, 2446, 3277, 3337
FspBI CTAG 4 cut(s) 1268, 1412, 1521, 1845
GluI GCNGC 9 cut(s) 231, 461, 748, 783, 879, 1257, 2446, 3277, 3337
GsaI CCCAGC 2 cut(s) 978, 1638
GsuI CTGGAG 4 cut(s) 1194, 1730, 1973, 2780
HaeIII GGCC 5 cut(s) 28, 53, 491, 635, 1103
HapII CCGG 3 cut(s) 29, 435, 1576
HgaI GACGC 1 cut(s) 2437
Hin1I GRCGYC 1 cut(s) 546
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 2 cut(s) 1893, 1955
HinfI GANTC 9 cut(s) 526, 970, 1094, 1136, 1493, 1517, 2831, 2914, 3187
HpaII CCGG 3 cut(s) 29, 435, 1576
HphI GGTGA 5 cut(s) 736, 1009, 1060, 2422, 2734
Hpy166II GTNNAC 8 cut(s) 544, 691, 740, 1190, 2127, 2290, 3052, 3315
Hpy8I GTNNAC 8 cut(s) 544, 691, 740, 1190, 2127, 2290, 3052, 3315
HpyAV CCTTC 8 cut(s) 646, 1159, 1642, 2168, 2807, 3058, 3159, 3232
HpyCH4III ACNGT 9 cut(s) 133, 1281, 2124, 2138, 2515, 2939, 3162, 3312, 3360
HpyCH4IV ACGT 5 cut(s) 385, 546, 884, 2899, 3325
HpyF10VI GCNNNNNNNGC 3 cut(s) 25, 620, 1205
HpyF3I CTNAG 9 cut(s) 72, 1073, 1161, 1605, 1835, 1967, 2027, 2840, 2873
HpySE526I ACGT 5 cut(s) 385, 546, 884, 2899, 3325
Hsp92I GRCGYC 1 cut(s) 546
LguI GCTCTTC 1 cut(s) 868
LmnI GCTCC 4 cut(s) 370, 985, 2588, 3013
Lsp1109I GCAGC 7 cut(s) 242, 734, 769, 1268, 2432, 3288, 3348
MaeI CTAG 4 cut(s) 1268, 1412, 1521, 1845
MaeII ACGT 5 cut(s) 385, 546, 884, 2899, 3325
MfeI CAATTG 2 cut(s) 588, 2993
MflI RGATCY 7 cut(s) 190, 315, 1785, 1831, 2354, 2807, 2860
MhlI GDGCHC 4 cut(s) 375, 742, 1222, 3023
MlsI TGGCCA 1 cut(s) 491
MluNI TGGCCA 1 cut(s) 491
MlyI GAGTC 3 cut(s) 520, 2840, 2923
MmeI TCCRAC 5 cut(s) 688, 1432, 1921, 2838, 3009
Mox20I TGGCCA 1 cut(s) 491
Mph1103I ATGCAT 3 cut(s) 722, 1312, 1566
MscI TGGCCA 1 cut(s) 491
MslI CAYNNNNRTG 4 cut(s) 735, 1315, 2375, 2468
Msp20I TGGCCA 1 cut(s) 491
MspA1I CMGCKG 2 cut(s) 2051, 3339
MspCI CTTAAG 2 cut(s) 1949, 2687
MspI CCGG 3 cut(s) 29, 435, 1576
MspR9I CCNGG 5 cut(s) 436, 1238, 1577, 1991, 2670
MunI CAATTG 2 cut(s) 588, 2993
Mva1269I GAATGC 4 cut(s) 1131, 1184, 1310, 1774
MvaI CCWGG 3 cut(s) 1238, 1991, 2670
MwoI GCNNNNNNNGC 3 cut(s) 25, 620, 1205
NciI CCSGG 2 cut(s) 436, 1577
NcoI CCATGG 2 cut(s) 636, 2482
NdeI CATATG 3 cut(s) 616, 2882, 3108
NlaIV GGNNCC 2 cut(s) 108, 372
NmeAIII GCCGAG 1 cut(s) 3004
NmuCI GTSAC 6 cut(s) 673, 1275, 2074, 2410, 2459, 3326
NsiI ATGCAT 3 cut(s) 722, 1312, 1566
NspI RCATGY 6 cut(s) 720, 734, 1157, 2702, 2870, 3235
NspV TTCGAA 1 cut(s) 609
PagI TCATGA 1 cut(s) 1047
PciI ACATGT 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 868
PctI GAATGC 4 cut(s) 1131, 1184, 1310, 1774
PfeI GAWTC 6 cut(s) 970, 1094, 1136, 1493, 1517, 3187
PflFI GACNNNGTC 2 cut(s) 671, 2465
PfoI TCCNGGA 2 cut(s) 1575, 1989
PkrI GCNGC 9 cut(s) 232, 462, 749, 784, 880, 1258, 2447, 3278, 3338
Ple19I CGATCG 1 cut(s) 148
PleI GAGTC 3 cut(s) 520, 2839, 2922
PpsI GAGTC 3 cut(s) 520, 2839, 2922
PpuMI RGGWCCY 1 cut(s) 107
PscI ACATGT 1 cut(s) 730
PsiI TTATAA 2 cut(s) 522, 923
Psp5II RGGWCCY 1 cut(s) 107
Psp6I CCWGG 3 cut(s) 1236, 1989, 2668
PspFI CCCAGC 2 cut(s) 974, 1634
PspGI CCWGG 3 cut(s) 1236, 1989, 2668
PspN4I GGNNCC 2 cut(s) 108, 372
PspPI GGNCC 3 cut(s) 107, 1101, 1354
PspPPI RGGWCCY 1 cut(s) 107
PstI CTGCAG 1 cut(s) 156
PstNI CAGNNNCTG 1 cut(s) 1496
PsuI RGATCY 7 cut(s) 190, 315, 1785, 1831, 2354, 2807, 2860
PsyI GACNNNGTC 2 cut(s) 671, 2465
PvuI CGATCG 1 cut(s) 148
PvuII CAGCTG 1 cut(s) 3339
RsaI GTAC 6 cut(s) 384, 1508, 1857, 2383, 3291, 3314
RsaNI GTAC 6 cut(s) 383, 1507, 1856, 2382, 3290, 3313
RseI CAYNNNNRTG 4 cut(s) 735, 1315, 2375, 2468
SapI GCTCTTC 1 cut(s) 868
SatI GCNGC 9 cut(s) 231, 461, 748, 783, 879, 1257, 2446, 3277, 3337
Sau96I GGNCC 3 cut(s) 107, 1101, 1354
SchI GAGTC 3 cut(s) 520, 2840, 2923
ScrFI CCNGG 5 cut(s) 436, 1238, 1577, 1991, 2670
SduI GDGCHC 4 cut(s) 375, 742, 1222, 3023
SfcI CTRYAG 1 cut(s) 152
SfuI TTCGAA 1 cut(s) 609
SinI GGWCC 2 cut(s) 107, 1354
SmiI ATTTAAAT 2 cut(s) 1779, 2775
SmiMI CAYNNNNRTG 4 cut(s) 735, 1315, 2375, 2468
SmlI CTYRAG 5 cut(s) 805, 1949, 2350, 2501, 2687
SmoI CTYRAG 5 cut(s) 805, 1949, 2350, 2501, 2687
SpeI ACTAGT 1 cut(s) 1844
SsiI CCGC 5 cut(s) 460, 463, 565, 879, 2049
SspMI CTAG 4 cut(s) 1268, 1412, 1521, 1845
StyD4I CCNGG 5 cut(s) 434, 1236, 1575, 1989, 2668
StyI CCWWGG 3 cut(s) 636, 2358, 2482
SwaI ATTTAAAT 2 cut(s) 1779, 2775
TaaI ACNGT 9 cut(s) 133, 1281, 2124, 2138, 2515, 2939, 3162, 3312, 3360
TaiI ACGT 5 cut(s) 388, 549, 887, 2902, 3328
TaqI TCGA 9 cut(s) 48, 148, 609, 1453, 1470, 2187, 2274, 2335, 3304
TatI WGTACW 3 cut(s) 1855, 3289, 3312
TauI GCSGC 2 cut(s) 463, 881
TfiI GAWTC 6 cut(s) 970, 1094, 1136, 1493, 1517, 3187
TscAI CASTG 9 cut(s) 80, 604, 682, 1698, 2206, 2518, 2598, 2877, 3023
TseFI GTSAC 6 cut(s) 673, 1275, 2074, 2410, 2459, 3326
TseI GCWGC 7 cut(s) 230, 747, 782, 1256, 2445, 3276, 3336
Tsp45I GTSAC 6 cut(s) 673, 1275, 2074, 2410, 2459, 3326
TspGWI ACGGA 1 cut(s) 1780
TspRI CASTG 9 cut(s) 80, 604, 682, 1698, 2206, 2518, 2598, 2877, 3023
Tth111I GACNNNGTC 2 cut(s) 671, 2465
Vha464I CTTAAG 2 cut(s) 1949, 2687
VneI GTGCAC 1 cut(s) 738
VpaK11BI GGWCC 2 cut(s) 107, 1354
XceI RCATGY 6 cut(s) 720, 734, 1157, 2702, 2870, 3235
XmiI GTMKAC 2 cut(s) 690, 2126
XspI CTAG 4 cut(s) 1268, 1412, 1521, 1845
ZraI GACGTC 1 cut(s) 547
Zsp2I ATGCAT 3 cut(s) 722, 1312, 1566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.