FvH4_3g45690

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37880387 .. 37885918
5532 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45690.t1

Sequence Viewer

Length: 3564 bp
ATGGAGTTCTATTTCTTCGAGATATGGTTGGCGATTGTTGCGGTAATTGTGACATGGATGGGTTGCAAGGCCATTAAGGGGATATGGACTCAAGTAAAGCGTCGGATAAGCGTCGGAAAGTCGCAGGATAATCAAGGAGTGGTTAACTTGAATGCCTCTGAATCTCTTGCTTCATCATCAGAACCTCGTCCCTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAAGGGATTACAGCCCAAATACGCGATCGACTTCGTAGAAGTGGCATTATAACGTTCATGGATGAAACAGATCTTAGAGTAGGGGATGTTATTTCTCCCACACTCATAAAGGCAATTAAAGAGTCAAGGTTTGCAATCGTAATTCTCTCGAAAAAATATGCTTCTTCTGTTTGGTGTTTGGAGGAACTTCGAGAGATTTGTCTATCCATGGAAGAGAGCAGAATTTTGCCACTTTTTTTTAAAATTGATCCTACGGATGTCCGATTTCAGAACAACAGTTTCAAAAAAGCTTTCTCTAAGCACGAAACCTCTGGGCGACATGCATCAGAGAAGGTGAATCAATGGAGAGCTGATTTATACAAAGTGGCCAATATCTCTGGGTGGAACACAAATGATTACAAGACTCATGAAGAACTTGTTGACAAGATTGTGGAATCTCTCCGCAGTAGAGTTACACCTGATGCAATTGAATTCACAGATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAGACAAGGTTATGAAGGCGCTAACAGATGACAAGATTACTGCCGTCGGTGTCTATGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTACTTTTGACAAATGGATTATGGCTGTTGTATCCCAAAAGCTTGACTTGGAAAAAGTTCAAGACACATTGGCAGAGCTGCTGGGCTTCGAATTAAAAGATAAGACAGAAGCTGGAAGAGCAGATAAGTTACATAAGGAGATCATGAGAAAAGAAAAACTCCTGATAATTGTGGACGATGTCTGGGAGAGAACAGAGTTGTCAAAGATAGGGATTCCCACCTACAATGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCATGAGGATATTGAATGTCTGTAATGCCATGAAGTGCCATGAAAAGATCAGACTCAAAGTTCTCTCAGATCAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTCGAGTCCACTTCGTTTAAGGAAGTGGCATGGAGGGTAGCTGGAGAGTGTAAGAATCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGCAGATGCGGACCAGGAGGAATGGGAGAAGGCAGCTGAACGACTAAAGCAGTCGCAACGTGTCAATCCTAACCATGAGGAAGACGCATTCCGATGTATAAGATTAAGCTATAATTACTTGAAAGATGGGGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATGACATCAAAATAGAAGACTTGTTCCGGTACGGGATCGGAAAAAGATTGTTTCGAGATGCCCAAACAATGGAAGAAGCCAGAGGAATAGTAGTTTCAGTGGTCAAGTACCTGAAAAATTCTAGCTTGCTTTTGGATAGCCAGAAAAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCACAATCTGAAGAGGGGCATCGGTTTTTTGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGCGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACGAAATTTGCAAGCTACCCGAAGAAGAGCTGGTATGTCAAAATCTCCAGATTTTATTACTAAACGGGAATGAGGAACTAAGTGAGATCCCAGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGCAAGACTAGTATTTCCGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCAAGCTTTGTATTTAAATAGTTGCGGCAAATTAGTTGACATTTCCGTTGTGGGGAAACTAAACAAGCTTGAAATTCTTAGTATGAGAGACTGTTCCTTGGTGGAATTGTCTAGAGAAATAGGACATTTGACCAATCTAAAGATTTTGGATGTCAATCGCCATTGGGGACATGGTATGGTTACAATTCCATCTACAGTGATATCAAAGTTGCAAGAATTAGAAGAATTGTACATGGTGTACTGTGGATTTGAAGAGGACGGGTCGTGTGTAGCTGAGGGACCATCAGAAAGAACCAATATTATTAGGTCTGATGAGTTAGCTGCTTTATCAAATTTGAAAATATTGCAGGTTGGCTTGTCTGATGAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCGGATTGGGATTACTTTTGTATAACTATCAATGGCATATCCTCGTCCCGTGAATACAAACAAGGAGATCGTAATTCAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCGGATTGGGATTACATTTGTTTAAATAAAGATGGCATATCCTCGTCCCGTGAATACAAACAAGGAGATTGTAATTCAAGATCCTTGTATCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTAAAAAAGCGGTGACAGAGAAAACAGAGAAGCTACAGTATGACCGCTGTAGAGGAATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTTGGGTTTTCTTTTGACAAGTTGAAAGAGTTGATTACCACAACAGGACGAGTTGAAACAGGACCCGTGTTTGAGAATTTGGAAGAGTTGCATCTGAGACATCTGATCTTCCTGGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTGTTTCATGTGCGTTTTGGTACGCTTTTGAAGAGTGTATCAAAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGATTTAAACTTGCTGTTCAAATTGGAATATGTGTTCAGATGCGAAGGGGACGAGCTAGAACAAGCAAAACTGAGAGAGATGCATTTGATGTCTCTAAATAAACTCAGAAGCATATGTAGTGGTCCTGCCCCACGTGCAATGTTCCAGAGTCTTAAGATTTTGACCGTGTGCAATTGTGATCTGCTGCAAAGTCTGTTCGCATCTGATGTGGCCCAATGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGTCGTGTTATTTGTTGCAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACGGTTCTACCAAAACTGAAGAACTTGGTTTTGATCGATCTTCCTAAGTTGTATAGTGCAATTGCTGCCGTTGATATTGAATGTCCTTCATTGGAACGCTTGATGGTGGTGGGATGCCCCGAGGTTTTATTTTCAACCTCTTCCGATCTCTTCGGAGCCTTCAAAAGCAGGAACCAATTTTCATTTTCAGCCTCCACTTCTCACCCAGTCCAACTCAATGATCCACAATTATACAAAACTCTACGTGGCAGAGCATCTTTGTGA

Protein Analysis

1188

Amino Acids

134.6

Weight (kDa)

7.05

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 66 - 229 5.2e-42 TIR domain
TIR_2 PF13676 69 - 163 3.8e-15 TIR domain
NB-ARC PF00931 249 - 412 1.2e-30 NB-ARC domain
LRR_8 PF13855 622 - 679 4.1e-09 Leucine rich repeat
LRR_14 PF23598 625 - 757 7.3e-16 Leucine-rich repeat region
LRR_RPS2 PF23247 947 - 1086 2.7e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
Acc36I ACCTGC 1 cut(s) 2350
AccB7I CCANNNNNTGG 1 cut(s) 1585
AccII CGCG 2 cut(s) 261, 1782
AciI CCGC 9 cut(s) 41, 679, 816, 1349, 1774, 1782, 2037, 2654, 2689
AclI AACGTT 1 cut(s) 290
AclWI GGATC 7 cut(s) 479, 1559, 1903, 1918, 1962, 2592, 3515
AcoI YGGCCR 2 cut(s) 603, 1775
AcsI RAATTY 8 cut(s) 459, 707, 1633, 1827, 2085, 2344, 2836, 2969
AcuI CTGAAG 2 cut(s) 1743, 3338
AcvI CACGTG 1 cut(s) 3137
AfaI GTAC 8 cut(s) 874, 1547, 1625, 2243, 2252, 2629, 2947, 2976
AfiI CCNNNNNNNGG 3 cut(s) 78, 1585, 2064
AflII CTTAAG 1 cut(s) 3155
AflIII ACRYGT 2 cut(s) 844, 1399
AhlI ACTAGT 1 cut(s) 1967
AjnI CCWGG 4 cut(s) 191, 1353, 2871, 2902
AjuI GAANNNNNNNTTGG 4 cut(s) 1469, 1501, 3210, 3242
AloI GAACNNNNNNTCC 2 cut(s) 3020, 3052
Alw26I GTCTC 7 cut(s) 835, 2003, 2094, 2851, 2913, 2974, 3099
AlwI GGATC 7 cut(s) 479, 1559, 1903, 1918, 1962, 2592, 3515
AlwNI CAGNNNCTG 2 cut(s) 983, 1933
Ama87I CYCGRG 1 cut(s) 3419
AoxI GGCC 4 cut(s) 69, 603, 1775, 3213
ApeKI GCWGC 7 cut(s) 851, 949, 1373, 1797, 2333, 3187, 3365
ApoI RAATTY 8 cut(s) 459, 707, 1633, 1827, 2085, 2344, 2836, 2969
Asp700I GAANNNNTTC 3 cut(s) 927, 1243, 3240
AspLEI GCGC 1 cut(s) 772
AspS9I GGNCC 7 cut(s) 224, 1215, 1351, 2291, 2822, 3125, 3214
AsuC2I CCSGG 1 cut(s) 1694
AsuHPI GGTGA 7 cut(s) 583, 850, 1123, 1996, 2668, 2903, 3494
AsuII TTCGAA 1 cut(s) 960
AvaI CYCGRG 1 cut(s) 3419
AvaII GGWCC 6 cut(s) 224, 1215, 1351, 2291, 2822, 3125
BaeGI GKGCMC 1 cut(s) 1339
BalI TGGCCA 1 cut(s) 605
BbrPI CACGTG 1 cut(s) 3137
BbsI GAAGAC 5 cut(s) 1428, 1518, 1539, 3243, 3305
BbvCI CCTCAGC 1 cut(s) 2286
BbvI GCAGC 7 cut(s) 838, 936, 1385, 1784, 2320, 3174, 3352
BccI CCATC 7 cut(s) 52, 1460, 2209, 2302, 2543, 2630, 3397
BceAI ACGGC 2 cut(s) 779, 3353
BciT130I CCWGG 4 cut(s) 193, 1355, 2873, 2904
BciVI GTATCC 2 cut(s) 913, 1690
BcnI CCSGG 1 cut(s) 1694
BcoDI GTCTC 7 cut(s) 835, 2003, 2094, 2851, 2913, 2974, 3099
BcuI ACTAGT 1 cut(s) 1967
BfaI CTAG 5 cut(s) 1638, 1968, 2124, 3002, 3059
BfmI CTRYAG 4 cut(s) 862, 2205, 2678, 2692
BfoI RGCGCY 1 cut(s) 773
BfrI CTTAAG 1 cut(s) 3155
BfuAI ACCTGC 1 cut(s) 2350
BfuI GTATCC 2 cut(s) 913, 1690
BglII AGATCT 1 cut(s) 307
Bme1390I CCNGG 5 cut(s) 193, 1355, 1694, 2873, 2904
Bme18I GGWCC 6 cut(s) 224, 1215, 1351, 2291, 2822, 3125
BmeT110I CYCGRG 1 cut(s) 3419
BmgT120I GGNCC 7 cut(s) 224, 1215, 1351, 2291, 2822, 3125, 3214
BmiI GGNNCC 5 cut(s) 225, 2292, 2824, 3457, 3473
BmrFI CCNGG 5 cut(s) 193, 1355, 1694, 2873, 2904
BmrI ACTGGG 1 cut(s) 3500
BmuI ACTGGG 1 cut(s) 3500
BpiI GAAGAC 5 cut(s) 1428, 1518, 1539, 3243, 3305
BpmI CTGGAG 4 cut(s) 1311, 1853, 2894, 3014
Bpu10I CCTNAGC 1 cut(s) 2286
Bpu14I TTCGAA 1 cut(s) 960
BpuEI CTTGAG 3 cut(s) 75, 2732, 2906
BpuMI CCSGG 1 cut(s) 1694
Bsa29I ATCGAT 1 cut(s) 3336
BsaAI YACGTR 2 cut(s) 3137, 3545
BsaBI GATNNNNATC 1 cut(s) 2166
BsaI GGTCTC 1 cut(s) 2913
BsaJI CCNNGG 6 cut(s) 191, 444, 2109, 2728, 2903, 3420
BsaWI WCCGGW 3 cut(s) 1542, 2410, 2518
BsaXI ACNNNNNCTCC 2 cut(s) 2867, 2897
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 1585, 2064
Bse1I ACTGG 1 cut(s) 3506
Bse3DI GCAATG 1 cut(s) 3147
Bse8I GATNNNNATC 1 cut(s) 2166
BseBI CCWGG 4 cut(s) 193, 1355, 2873, 2904
BseCI ATCGAT 1 cut(s) 3336
BseDI CCNNGG 6 cut(s) 191, 444, 2109, 2728, 2903, 3420
BseJI GATNNNNATC 1 cut(s) 2166
BseLI CCNNNNNNNGG 3 cut(s) 78, 1585, 2064
BseMI GCAATG 1 cut(s) 3147
BseMII CTCAG 5 cut(s) 1212, 2277, 2846, 3065, 3121
BseNI ACTGG 1 cut(s) 3506
BseRI GAGGAG 2 cut(s) 1991, 2891
BseSI GKGCMC 1 cut(s) 1339
BseXI GCAGC 7 cut(s) 838, 936, 1385, 1784, 2320, 3174, 3352
BseYI CCCAGC 1 cut(s) 952
BsgI GTGCAG 2 cut(s) 837, 1783
Bsh1236I CGCG 2 cut(s) 261, 1782
Bsh1285I CGRYCG 1 cut(s) 265
BshFI GGCC 4 cut(s) 71, 605, 1777, 3215
BshVI ATCGAT 1 cut(s) 3336
BsiEI CGRYCG 1 cut(s) 265
BsiHKCI CYCGRG 1 cut(s) 3419
BsiSI CCGG 4 cut(s) 1543, 1693, 2411, 2519
BslFI GGGAC 6 cut(s) 174, 2193, 2304, 2440, 2548, 3065
BslI CCNNNNNNNGG 3 cut(s) 78, 1585, 2064
BsmAI GTCTC 7 cut(s) 835, 2003, 2094, 2851, 2913, 2974, 3099
BsmFI GGGAC 6 cut(s) 174, 2193, 2304, 2440, 2548, 3065
BsmI GAATGC 2 cut(s) 157, 1427
BsnI GGCC 4 cut(s) 71, 605, 1777, 3215
Bso31I GGTCTC 1 cut(s) 2913
BsoBI CYCGRG 1 cut(s) 3419
Bsp119I TTCGAA 1 cut(s) 960
Bsp1286I GDGCHC 1 cut(s) 1339
Bsp1407I TGTACA 2 cut(s) 2241, 2974
Bsp19I CCATGG 2 cut(s) 444, 2728
BspACI CCGC 9 cut(s) 41, 679, 816, 1349, 1774, 1782, 2037, 2654, 2689
BspANI GGCC 4 cut(s) 71, 605, 1777, 3215
BspCNI CTCAG 5 cut(s) 1211, 2278, 2847, 3066, 3120
BspDI ATCGAT 1 cut(s) 3336
BspFNI CGCG 2 cut(s) 261, 1782
BspHI TCATGA 2 cut(s) 643, 1014
BspLI GGNNCC 5 cut(s) 225, 2292, 2824, 3457, 3473
BspMAI CTGCAG 1 cut(s) 866
BspMI ACCTGC 1 cut(s) 2350
BspPI GGATC 7 cut(s) 479, 1559, 1903, 1918, 1962, 2592, 3515
BspQI GCTCTTC 2 cut(s) 982, 1842
BspT104I TTCGAA 1 cut(s) 960
BspTI CTTAAG 1 cut(s) 3155
BspTNI GGTCTC 1 cut(s) 2913
BsrDI GCAATG 1 cut(s) 3147
BsrGI TGTACA 2 cut(s) 2241, 2974
BsrI ACTGG 1 cut(s) 3506
BssECI CCNNGG 6 cut(s) 191, 444, 2109, 2728, 2903, 3420
BssT1I CCWWGG 3 cut(s) 444, 2109, 2728
Bst2UI CCWGG 4 cut(s) 193, 1355, 2873, 2904
Bst4CI ACNGT 8 cut(s) 515, 2105, 2209, 2255, 2682, 2761, 3169, 3304
Bst6I CTCTTC 9 cut(s) 444, 982, 1719, 1842, 2259, 2838, 2951, 3445, 3455
BstAFI CTTAAG 1 cut(s) 3155
BstAPI GCANNNNNTGC 1 cut(s) 3365
BstAUI TGTACA 2 cut(s) 2241, 2974
BstBAI YACGTR 2 cut(s) 3137, 3545
BstBI TTCGAA 1 cut(s) 960
BstC8I GCNNGC 4 cut(s) 862, 1643, 1753, 1835
BstDSI CCRYGG 2 cut(s) 444, 2728
BstFNI CGCG 2 cut(s) 261, 1782
BstH2I RGCGCY 1 cut(s) 773
BstHHI GCGC 1 cut(s) 772
BstMAI GTCTC 7 cut(s) 835, 2003, 2094, 2851, 2913, 2974, 3099
BstMCI CGRYCG 1 cut(s) 265
BstMWI GCNNNNNNNGC 8 cut(s) 38, 734, 816, 857, 989, 1322, 3137, 3365
BstNI CCWGG 4 cut(s) 193, 1355, 2873, 2904
BstNSI RCATGY 2 cut(s) 560, 848
BstSCI CCNGG 5 cut(s) 191, 1353, 1692, 2871, 2902
BstSFI CTRYAG 4 cut(s) 862, 2205, 2678, 2692
BstSLI GKGCMC 1 cut(s) 1339
BstUI CGCG 2 cut(s) 261, 1782
BstV1I GCAGC 7 cut(s) 838, 936, 1385, 1784, 2320, 3174, 3352
BstV2I GAAGAC 5 cut(s) 1428, 1518, 1539, 3243, 3305
BstX2I RGATCY 4 cut(s) 307, 1908, 1954, 2597
BstXI CCANNNNNNTGG 1 cut(s) 731
BstYI RGATCY 4 cut(s) 307, 1908, 1954, 2597
Bsu15I ATCGAT 1 cut(s) 3336
BsuI GTATCC 2 cut(s) 913, 1690
BsuRI GGCC 4 cut(s) 71, 605, 1777, 3215
BsuTUI ATCGAT 1 cut(s) 3336
BtgI CCRYGG 2 cut(s) 444, 2728
BtsI GCAGTG 2 cut(s) 1800, 3291
BtsIMutI CAGTG 4 cut(s) 1620, 1800, 2214, 3291
BveI ACCTGC 1 cut(s) 2350
Cac8I GCNNGC 4 cut(s) 862, 1643, 1753, 1835
CaiI CAGNNNCTG 2 cut(s) 983, 1933
CciI TCATGA 2 cut(s) 643, 1014
CfoI GCGC 1 cut(s) 772
Cfr13I GGNCC 7 cut(s) 224, 1215, 1351, 2291, 2822, 3125, 3214
ClaI ATCGAT 1 cut(s) 3336
CseI GACGC 3 cut(s) 89, 100, 1433
Csp6I GTAC 8 cut(s) 873, 1546, 1624, 2242, 2251, 2628, 2946, 2975
CviQI GTAC 8 cut(s) 873, 1546, 1624, 2242, 2251, 2628, 2946, 2975
DraI TTTAAA 6 cut(s) 478, 1764, 2028, 2541, 2647, 3009
EaeI YGGCCR 2 cut(s) 603, 1775
Eam1104I CTCTTC 9 cut(s) 444, 982, 1719, 1842, 2259, 2838, 2951, 3445, 3455
EarI CTCTTC 9 cut(s) 444, 982, 1719, 1842, 2259, 2838, 2951, 3445, 3455
Eco130I CCWWGG 3 cut(s) 444, 2109, 2728
Eco31I GGTCTC 1 cut(s) 2913
Eco32I GATATC 1 cut(s) 2214
Eco47I GGWCC 6 cut(s) 224, 1215, 1351, 2291, 2822, 3125
Eco57I CTGAAG 2 cut(s) 1743, 3338
Eco72I CACGTG 1 cut(s) 3137
Eco88I CYCGRG 1 cut(s) 3419
EcoO109I RGGNCCY 2 cut(s) 224, 2822
EcoRI GAATTC 1 cut(s) 707
EcoRII CCWGG 4 cut(s) 191, 1353, 2871, 2902
EcoRV GATATC 1 cut(s) 2214
EcoT14I CCWWGG 3 cut(s) 444, 2109, 2728
EcoT22I ATGCAT 3 cut(s) 562, 1683, 3087
ErhI CCWWGG 3 cut(s) 444, 2109, 2728
FalI AAGNNNNNCTT 6 cut(s) 902, 934, 1469, 1501, 3228, 3260
FaqI GGGAC 6 cut(s) 174, 2193, 2304, 2440, 2548, 3065
FauNDI CATATG 1 cut(s) 3116
FspBI CTAG 5 cut(s) 1638, 1968, 2124, 3002, 3059
GlaI GCGC 1 cut(s) 771
GsaI CCCAGC 1 cut(s) 956
GsuI CTGGAG 4 cut(s) 1311, 1853, 2894, 3014
HaeII RGCGCY 1 cut(s) 773
HaeIII GGCC 4 cut(s) 71, 605, 1777, 3215
HapII CCGG 4 cut(s) 1543, 1693, 2411, 2519
HgaI GACGC 3 cut(s) 89, 100, 1433
HhaI GCGC 1 cut(s) 772
Hin6I GCGC 1 cut(s) 770
HinP1I GCGC 1 cut(s) 770
HincII GTYRAC 3 cut(s) 145, 658, 2050
HindII GTYRAC 3 cut(s) 145, 658, 2050
HindIII AAGCTT 4 cut(s) 525, 911, 2016, 2078
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 4 cut(s) 1543, 1693, 2411, 2519
HphI GGTGA 7 cut(s) 583, 850, 1123, 1996, 2668, 2903, 3494
Hpy166II GTNNAC 7 cut(s) 145, 658, 1045, 1257, 2050, 2251, 3289
Hpy8I GTNNAC 7 cut(s) 145, 658, 1045, 1257, 2050, 2251, 3289
Hpy99I CGWCG 3 cut(s) 105, 116, 800
HpyAV CCTTC 8 cut(s) 562, 760, 1363, 1787, 3041, 3251, 3396, 3469
HpyCH4III ACNGT 8 cut(s) 515, 2105, 2209, 2255, 2682, 2761, 3169, 3304
HpyCH4IV ACGT 4 cut(s) 290, 1399, 3136, 3544
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 734, 816, 857, 989, 1322, 3137, 3365
HpySE526I ACGT 4 cut(s) 290, 1399, 3136, 3544
HspAI GCGC 1 cut(s) 770
KspAI GTTAAC 1 cut(s) 145
LguI GCTCTTC 2 cut(s) 982, 1842
LmnI GCTCC 3 cut(s) 734, 2615, 3455
Lsp1109I GCAGC 7 cut(s) 838, 936, 1385, 1784, 2320, 3174, 3352
MaeI CTAG 5 cut(s) 1638, 1968, 2124, 3002, 3059
MaeII ACGT 4 cut(s) 290, 1399, 3136, 3544
MaeIII GTNAC 8 cut(s) 49, 688, 999, 2191, 2656, 2705, 2736, 2895
MfeI CAATTG 4 cut(s) 702, 3175, 3230, 3360
MflI RGATCY 4 cut(s) 307, 1908, 1954, 2597
MhlI GDGCHC 1 cut(s) 1339
MlsI TGGCCA 1 cut(s) 605
MluNI TGGCCA 1 cut(s) 605
MlyI GAGTC 7 cut(s) 82, 368, 634, 1179, 1262, 3160, 3260
MmeI TCCRAC 4 cut(s) 83, 94, 802, 3535
Mox20I TGGCCA 1 cut(s) 605
Mph1103I ATGCAT 3 cut(s) 562, 1683, 3087
MroXI GAANNNNTTC 3 cut(s) 927, 1243, 3240
MscI TGGCCA 1 cut(s) 605
MslI CAYNNNNRTG 3 cut(s) 1432, 2714, 3559
Msp20I TGGCCA 1 cut(s) 605
MspA1I CMGCKG 2 cut(s) 1376, 2691
MspCI CTTAAG 1 cut(s) 3155
MspI CCGG 4 cut(s) 1543, 1693, 2411, 2519
MspR9I CCNGG 5 cut(s) 193, 1355, 1694, 2873, 2904
MunI CAATTG 4 cut(s) 702, 3175, 3230, 3360
Mva1269I GAATGC 2 cut(s) 157, 1427
MvaI CCWGG 4 cut(s) 193, 1355, 2873, 2904
MvnI CGCG 2 cut(s) 261, 1782
MwoI GCNNNNNNNGC 8 cut(s) 38, 734, 816, 857, 989, 1322, 3137, 3365
NciI CCSGG 1 cut(s) 1694
NcoI CCATGG 2 cut(s) 444, 2728
NdeI CATATG 1 cut(s) 3116
NlaIV GGNNCC 5 cut(s) 225, 2292, 2824, 3457, 3473
NmuCI GTSAC 3 cut(s) 49, 2656, 2705
NsiI ATGCAT 3 cut(s) 562, 1683, 3087
NspI RCATGY 2 cut(s) 560, 848
NspV TTCGAA 1 cut(s) 960
PagI TCATGA 2 cut(s) 643, 1014
PciI ACATGT 1 cut(s) 844
PciSI GCTCTTC 2 cut(s) 982, 1842
PctI GAATGC 2 cut(s) 157, 1427
PdmI GAANNNNTTC 3 cut(s) 927, 1243, 3240
PfeI GAWTC 8 cut(s) 161, 574, 671, 1084, 1208, 1303, 1472, 1930
PflFI GACNNNGTC 1 cut(s) 1049
PflMI CCANNNNNTGG 1 cut(s) 1585
PfoI TCCNGGA 2 cut(s) 1692, 2871
Ple19I CGATCG 1 cut(s) 265
PleI GAGTC 7 cut(s) 82, 367, 634, 1179, 1261, 3159, 3259
PmaCI CACGTG 1 cut(s) 3137
PmlI CACGTG 1 cut(s) 3137
PpsI GAGTC 7 cut(s) 82, 367, 634, 1179, 1261, 3159, 3259
Ppu21I YACGTR 2 cut(s) 3137, 3545
PpuMI RGGWCCY 2 cut(s) 224, 2822
PscI ACATGT 1 cut(s) 844
PsiI TTATAA 1 cut(s) 287
Psp1406I AACGTT 1 cut(s) 290
Psp5II RGGWCCY 2 cut(s) 224, 2822
Psp6I CCWGG 4 cut(s) 191, 1353, 2871, 2902
PspCI CACGTG 1 cut(s) 3137
PspFI CCCAGC 1 cut(s) 952
PspGI CCWGG 4 cut(s) 191, 1353, 2871, 2902
PspN4I GGNNCC 5 cut(s) 225, 2292, 2824, 3457, 3473
PspPI GGNCC 7 cut(s) 224, 1215, 1351, 2291, 2822, 3125, 3214
PspPPI RGGWCCY 2 cut(s) 224, 2822
PstI CTGCAG 1 cut(s) 866
PstNI CAGNNNCTG 2 cut(s) 983, 1933
PsuI RGATCY 4 cut(s) 307, 1908, 1954, 2597
PsyI GACNNNGTC 1 cut(s) 1049
PvuI CGATCG 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 1376
RsaI GTAC 8 cut(s) 874, 1547, 1625, 2243, 2252, 2629, 2947, 2976
RsaNI GTAC 8 cut(s) 873, 1546, 1624, 2242, 2251, 2628, 2946, 2975
RseI CAYNNNNRTG 3 cut(s) 1432, 2714, 3559
SapI GCTCTTC 2 cut(s) 982, 1842
Sau96I GGNCC 7 cut(s) 224, 1215, 1351, 2291, 2822, 3125, 3214
SchI GAGTC 7 cut(s) 82, 368, 634, 1179, 1262, 3160, 3260
ScrFI CCNGG 5 cut(s) 193, 1355, 1694, 2873, 2904
SduI GDGCHC 1 cut(s) 1339
SfcI CTRYAG 4 cut(s) 862, 2205, 2678, 2692
SfuI TTCGAA 1 cut(s) 960
SinI GGWCC 6 cut(s) 224, 1215, 1351, 2291, 2822, 3125
SmiI ATTTAAAT 1 cut(s) 2028
SmiMI CAYNNNNRTG 3 cut(s) 1432, 2714, 3559
SmlI CTYRAG 4 cut(s) 90, 2747, 2921, 3155
SmoI CTYRAG 4 cut(s) 90, 2747, 2921, 3155
SpeI ACTAGT 1 cut(s) 1967
SsiI CCGC 9 cut(s) 41, 679, 816, 1349, 1774, 1782, 2037, 2654, 2689
SspI AATATT 2 cut(s) 2311, 2355
SspMI CTAG 5 cut(s) 1638, 1968, 2124, 3002, 3059
StyD4I CCNGG 5 cut(s) 191, 1353, 1692, 2871, 2902
StyI CCWWGG 3 cut(s) 444, 2109, 2728
SwaI ATTTAAAT 1 cut(s) 2028
TaaI ACNGT 8 cut(s) 515, 2105, 2209, 2255, 2682, 2761, 3169, 3304
TaiI ACGT 4 cut(s) 293, 1402, 3139, 3547
TaqI TCGA 8 cut(s) 18, 265, 386, 427, 960, 1251, 1570, 3336
TatI WGTACW 3 cut(s) 2241, 2250, 2974
TauI GCSGC 3 cut(s) 819, 1777, 2040
TfiI GAWTC 8 cut(s) 161, 574, 671, 1084, 1208, 1303, 1472, 1930
TscAI CASTG 4 cut(s) 1620, 1807, 2214, 3291
TseFI GTSAC 3 cut(s) 49, 2656, 2705
TseI GCWGC 7 cut(s) 851, 949, 1373, 1797, 2333, 3187, 3365
Tsp45I GTSAC 3 cut(s) 49, 2656, 2705
TspGWI ACGGA 3 cut(s) 506, 1966, 2047
TspRI CASTG 4 cut(s) 1620, 1807, 2214, 3291
Tth111I GACNNNGTC 1 cut(s) 1049
Van91I CCANNNNNTGG 1 cut(s) 1585
Vha464I CTTAAG 1 cut(s) 3155
VpaK11BI GGWCC 6 cut(s) 224, 1215, 1351, 2291, 2822, 3125
XapI RAATTY 8 cut(s) 459, 707, 1633, 1827, 2085, 2344, 2836, 2969
XbaI TCTAGA 1 cut(s) 2123
XceI RCATGY 2 cut(s) 560, 848
XcmI CCANNNNNNNNNTGG 1 cut(s) 2180
XmnI GAANNNNTTC 3 cut(s) 927, 1243, 3240
XspI CTAG 5 cut(s) 1638, 1968, 2124, 3002, 3059
Zsp2I ATGCAT 3 cut(s) 562, 1683, 3087
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.