RLG00000002871

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000379
Physical Location & Seq
Reverse (-)
40934914 .. 40936062
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000002871

Sequence Viewer

Length: 981 bp
ATGGATCAGCTTATGGAGGCATTAAAAGATAACGAGGTCACTGCCGTTGGGACAACCCTTGTGGAACATGTCGGTCAAGAAGCTCGGAAGCAAGGCCTTTTTGATCATGTGATTATGGTTACAGTTAATCAAACCCCTGATTTGAAAAGAATTCAAGGCGTATTGGCAGATAGGCTAGGCATCAAATTGATGAGCGAGACAGATGAGGGAAGAGCTTCTAGATTGTATTCAGAGATAATGAGAAGAAAGAAAACCCTTATAATCTTGGACAACGTTTGGGATATATTAGACCTGCCAAGCGTAGGAATTCCCAGTTACAAGATGCTTCAGAGTCGCAACTCCAAAGTCCTACTTACCACAAGGAGACGAAATGCTTGTCATGTCATGAGGAGCCAAAAAAATATCACCCTCAATAGTCTGTCGAAAGAAGATTCATGGACATTGTTTGTGAAATATGCAAGAATGTCTTTTGAATCCACAGATTTCGAGGATGTCGCACGGAAGGTAGCTCGAGAATGTGGTGGTCTACTAATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGACCTGACAGAGTGGAAACACGCAGCTCAACGGCTAAAGAAGTCGCAAGCTCCCAGCCTTGACGATAAGGGAGATGTATCCAAATGCATAAAATTAAGCTATGATTTCTTGAAAGATGAGGACTCCAAGTCATGCTTCTTGCTTTGTTGCCTATTCCCAGAAGGCTATGACATCCAAATAGAAGACTTGTTCAAGATGCATGATATCATCCGGGATACAGCTATTCAAATTACACAATCTGAAGGTGAGGACGGATTTCTGGTAAAAGCTGGCTGTGTTTTGAAGATTTGGCCACGTCTATTGGGTGAAGGCCACACTGCAATTTCACTCATGAAGAACGACATTTGTGGGCTACCTGAAGAGCTGGTATATATGTCCAAAACTCCAAATTTTATTGCTACAAGAGAATGA

Protein Analysis

327

Amino Acids

36.88

Weight (kDa)

6.62

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 18 - 154 1.3e-20 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 260
Acc36I ACCTGC 1 cut(s) 300
AccI GTMKAC 1 cut(s) 526
AclI AACGTT 1 cut(s) 273
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 860
AcsI RAATTY 3 cut(s) 150, 306, 958
AcuI CTGAAG 3 cut(s) 311, 831, 948
AfiI CCNNNNNNNGG 1 cut(s) 302
AflIII ACRYGT 1 cut(s) 67
AgsI TTSAA 7 cut(s) 145, 155, 473, 682, 763, 797, 853
AhdI GACNNNNNGTC 1 cut(s) 697
AjiI CACGTC 1 cut(s) 866
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw26I GTCTC 2 cut(s) 191, 358
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 510
AoxI GGCC 3 cut(s) 94, 860, 880
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 3 cut(s) 150, 306, 958
ArsI GACNNNNNNTTYG 2 cut(s) 361, 393
Asp700I GAANNNNTTC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 782
AsuHPI GGTGA 3 cut(s) 397, 827, 887
AvaI CYCGRG 1 cut(s) 510
BaeGI GKGCMC 1 cut(s) 559
BalI TGGCCA 1 cut(s) 862
BbsI GAAGAC 1 cut(s) 759
BbvI GCAGC 1 cut(s) 605
BceAI ACGGC 2 cut(s) 29, 617
BcgI CGANNNNNNTGC 2 cut(s) 23, 57
BciVI GTATCC 2 cut(s) 658, 778
BclI TGATCA 1 cut(s) 103
BcnI CCSGG 1 cut(s) 782
BcoDI GTCTC 2 cut(s) 191, 358
BfaI CTAG 2 cut(s) 176, 219
BfuAI ACCTGC 1 cut(s) 300
BfuI GTATCC 2 cut(s) 658, 778
BisI GCNGC 1 cut(s) 594
BlsI GCNGC 1 cut(s) 595
Bme1390I CCNGG 1 cut(s) 782
BmeRI GACNNNNNGTC 1 cut(s) 697
BmeT110I CYCGRG 1 cut(s) 510
BmgBI CACGTC 1 cut(s) 866
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 782
BmrI ACTGGG 1 cut(s) 306
BmsI GCATC 3 cut(s) 189, 312, 756
BmuI ACTGGG 1 cut(s) 306
BpiI GAAGAC 1 cut(s) 759
BpuMI CCSGG 1 cut(s) 782
Bsc4I CCNNNNNNNGG 1 cut(s) 302
Bse1I ACTGG 1 cut(s) 312
BseGI GGATG 3 cut(s) 496, 741, 777
BseLI CCNNNNNNNGG 1 cut(s) 302
BseNI ACTGG 1 cut(s) 312
BseRI GAGGAG 1 cut(s) 403
BseSI GKGCMC 1 cut(s) 559
BseXI GCAGC 1 cut(s) 605
BseYI CCCAGC 1 cut(s) 623
BshFI GGCC 3 cut(s) 96, 862, 882
BsiHKCI CYCGRG 1 cut(s) 510
BsiSI CCGG 1 cut(s) 781
BslFI GGGAC 1 cut(s) 64
BslI CCNNNNNNNGG 1 cut(s) 302
BsmAI GTCTC 2 cut(s) 191, 358
BsmBI CGTCTC 1 cut(s) 358
BsmFI GGGAC 1 cut(s) 64
BsnI GGCC 3 cut(s) 96, 862, 882
BsoBI CYCGRG 1 cut(s) 510
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 2 cut(s) 4, 103
BspANI GGCC 3 cut(s) 96, 862, 882
BspHI TCATGA 2 cut(s) 384, 900
BspLI GGNNCC 1 cut(s) 392
BspMI ACCTGC 1 cut(s) 300
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 2 cut(s) 205, 924
BsrI ACTGG 1 cut(s) 312
BssMI GATC 2 cut(s) 4, 103
Bst4CI ACNGT 1 cut(s) 124
Bst6I CTCTTC 2 cut(s) 205, 924
BstC8I GCNNGC 2 cut(s) 618, 841
BstF5I GGATG 3 cut(s) 496, 741, 777
BstKTI GATC 2 cut(s) 7, 106
BstMAI GTCTC 2 cut(s) 191, 358
BstMBI GATC 2 cut(s) 4, 103
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstNSI RCATGY 1 cut(s) 71
BstSCI CCNGG 1 cut(s) 780
BstSLI GKGCMC 1 cut(s) 559
BstV1I GCAGC 1 cut(s) 605
BstV2I GAAGAC 1 cut(s) 759
BsuI GTATCC 2 cut(s) 658, 778
BsuRI GGCC 3 cut(s) 96, 862, 882
BtrI CACGTC 1 cut(s) 866
BtsCI GGATG 3 cut(s) 496, 741, 777
BtsI GCAGTG 2 cut(s) 39, 885
BtsIMutI CAGTG 2 cut(s) 39, 885
BveI ACCTGC 1 cut(s) 300
Cac8I GCNNGC 2 cut(s) 618, 841
CciI TCATGA 2 cut(s) 384, 900
CspCI CAANNNNNGTGG 4 cut(s) 42, 77, 852, 887
CviAII CATG 8 cut(s) 68, 107, 380, 385, 435, 702, 770, 901
DpnI GATC 2 cut(s) 6, 105
DpnII GATC 2 cut(s) 4, 103
DriI GACNNNNNGTC 1 cut(s) 697
EaeI YGGCCR 1 cut(s) 860
Eam1104I CTCTTC 2 cut(s) 205, 924
Eam1105I GACNNNNNGTC 1 cut(s) 697
EarI CTCTTC 2 cut(s) 205, 924
Eco147I AGGCCT 1 cut(s) 96
Eco32I GATATC 1 cut(s) 775
Eco57I CTGAAG 3 cut(s) 311, 831, 948
Eco88I CYCGRG 1 cut(s) 510
EcoRI GAATTC 2 cut(s) 150, 306
EcoRV GATATC 1 cut(s) 775
EcoT22I ATGCAT 2 cut(s) 659, 771
Esp3I CGTCTC 1 cut(s) 358
FaeI CATG 8 cut(s) 71, 110, 383, 388, 438, 705, 773, 904
FalI AAGNNNNNCTT 6 cut(s) 336, 368, 451, 483, 689, 721
FaqI GGGAC 1 cut(s) 64
FatI CATG 8 cut(s) 67, 106, 379, 384, 434, 701, 769, 900
FbaI TGATCA 1 cut(s) 103
FblI GTMKAC 1 cut(s) 526
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 3 cut(s) 503, 728, 764
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 2 cut(s) 176, 219
GluI GCNGC 1 cut(s) 594
GsaI CCCAGC 1 cut(s) 627
HaeIII GGCC 3 cut(s) 96, 862, 882
HapII CCGG 1 cut(s) 781
Hin1II CATG 8 cut(s) 71, 110, 383, 388, 438, 705, 773, 904
HinfI GANTC 4 cut(s) 331, 431, 473, 692
HpaII CCGG 1 cut(s) 781
HphI GGTGA 3 cut(s) 397, 827, 887
Hpy166II GTNNAC 1 cut(s) 527
Hpy188I TCNGA 4 cut(s) 87, 232, 330, 811
Hpy188III TCNNGA 7 cut(s) 77, 219, 385, 512, 679, 763, 901
Hpy8I GTNNAC 1 cut(s) 527
HpyAV CCTTC 4 cut(s) 496, 725, 806, 872
HpyCH4III ACNGT 1 cut(s) 124
HpyCH4IV ACGT 2 cut(s) 273, 865
HpyCH4V TGCA 6 cut(s) 458, 536, 551, 657, 769, 890
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpySE526I ACGT 2 cut(s) 273, 865
Hsp92II CATG 8 cut(s) 71, 110, 383, 388, 438, 705, 773, 904
Ksp22I TGATCA 1 cut(s) 103
Kzo9I GATC 2 cut(s) 4, 103
LguI GCTCTTC 2 cut(s) 205, 924
LmnI GCTCC 2 cut(s) 390, 625
Lsp1109I GCAGC 1 cut(s) 605
LweI GCATC 3 cut(s) 189, 312, 756
MaeI CTAG 2 cut(s) 176, 219
MaeII ACGT 2 cut(s) 273, 865
MaeIII GTNAC 3 cut(s) 37, 118, 314
MalI GATC 2 cut(s) 6, 105
MboI GATC 2 cut(s) 4, 103
MboII GAAGA 7 cut(s) 222, 255, 440, 764, 865, 916, 941
MhlI GDGCHC 1 cut(s) 559
MlsI TGGCCA 1 cut(s) 862
MluCI AATT 8 cut(s) 150, 185, 306, 531, 662, 798, 891, 958
MluNI TGGCCA 1 cut(s) 862
MlyI GAGTC 2 cut(s) 340, 686
MnlI CCTC 8 cut(s) 10, 28, 199, 381, 419, 481, 682, 811
Mox20I TGGCCA 1 cut(s) 862
Mph1103I ATGCAT 2 cut(s) 659, 771
MroXI GAANNNNTTC 1 cut(s) 214
MscI TGGCCA 1 cut(s) 862
MseI TTAA 3 cut(s) 23, 126, 665
Msp20I TGGCCA 1 cut(s) 862
MspI CCGG 1 cut(s) 781
MspR9I CCNGG 1 cut(s) 782
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 1 cut(s) 782
NdeII GATC 2 cut(s) 4, 103
NlaIII CATG 8 cut(s) 71, 110, 383, 388, 438, 705, 773, 904
NlaIV GGNNCC 1 cut(s) 392
NmuCI GTSAC 1 cut(s) 37
NsiI ATGCAT 2 cut(s) 659, 771
NspI RCATGY 1 cut(s) 71
PaeR7I CTCGAG 1 cut(s) 510
PagI TCATGA 2 cut(s) 384, 900
PceI AGGCCT 1 cut(s) 96
PciI ACATGT 1 cut(s) 67
PciSI GCTCTTC 2 cut(s) 205, 924
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 431, 473
PfoI TCCNGGA 1 cut(s) 780
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 2 cut(s) 339, 686
PpsI GAGTC 2 cut(s) 339, 686
PscI ACATGT 1 cut(s) 67
PsiI TTATAA 1 cut(s) 260
Psp1406I AACGTT 1 cut(s) 273
PspFI CCCAGC 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 392
SapI GCTCTTC 2 cut(s) 205, 924
SaqAI TTAA 3 cut(s) 23, 126, 665
SatI GCNGC 1 cut(s) 594
Sau3AI GATC 2 cut(s) 4, 103
SchI GAGTC 2 cut(s) 340, 686
ScrFI CCNGG 1 cut(s) 782
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 3 cut(s) 189, 312, 756
Sfr274I CTCGAG 1 cut(s) 510
SlaI CTCGAG 1 cut(s) 510
SmlI CTYRAG 1 cut(s) 510
SmoI CTYRAG 1 cut(s) 510
Sse9I AATT 8 cut(s) 150, 185, 306, 531, 662, 798, 891, 958
SseBI AGGCCT 1 cut(s) 96
SspMI CTAG 2 cut(s) 176, 219
StuI AGGCCT 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 780
TaaI ACNGT 1 cut(s) 124
TaiI ACGT 2 cut(s) 276, 868
TaqI TCGA 3 cut(s) 422, 486, 511
TaqII GACCGA 1 cut(s) 62
TasI AATT 8 cut(s) 150, 185, 306, 531, 662, 798, 891, 958
TfiI GAWTC 2 cut(s) 431, 473
Tru1I TTAA 3 cut(s) 23, 126, 665
Tru9I TTAA 3 cut(s) 23, 126, 665
TscAI CASTG 2 cut(s) 46, 892
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 423, 917
TspGWI ACGGA 2 cut(s) 514, 837
TspRI CASTG 2 cut(s) 46, 892
XapI RAATTY 3 cut(s) 150, 306, 958
XbaI TCTAGA 1 cut(s) 218
XceI RCATGY 1 cut(s) 71
XhoI CTCGAG 1 cut(s) 510
XmiI GTMKAC 1 cut(s) 526
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 2 cut(s) 176, 219
Zsp2I ATGCAT 2 cut(s) 659, 771
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.