RchiOBHm_Chr1g0326201

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000379
Physical Location & Seq
Forward (+)
14428883 .. 14430425
1543 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ55584

Sequence Viewer

Length: 1497 bp
ATGTCTAACGGAGATTTTGGATTATTTAAAGCAACAAGAAAAGCCATGGATAAGGTTATGAAGGAGCTAAAAGATGACAAGGTCACTGTCATTGGTGTCTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTGAAACATGTCGCTGCAGAATCCTGCAAAAATGGGACTTTTAACCATGTGATTATGGCTGTTGTATCCCAAAGCCCCAACTTCGAAAGAATTCAACGCACATTATTGGTGGAGCTACTGGCCCCCGGCTTCGAGTTACCAGAAAGTGGAAGAGCCTCTAGATTGTATAAAGAGATAATGAGGAAAAACAACATCCTCATAATCTTGGACGACATTTGGGAGTTTATAGATTTGTCAACAATAGGAATTCCTAGCCCCGACAGGCTCAAAAAACAGAATTCCAAACTCCTGCTCACCTCTAGGAGATTGCATGTTTGTAATTCCATGAGCCAAAAAAGCATTCGTCTCGATATATTATCAGAACAGGATTCTTGGAGCTTGTTTGTGAAGAAAACAAAAAGATCTTTTGAATTCCCCAACTTTGATAATGTAGCGAGGAAGGTAGCAAGAGAATGTAGGGGTCTACCCATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGATATGGAGGAATGGCAAAAAGCAGCTCAACAATTAGAGAAGTCGCAAATTGCCAACCCTGACCATGAAGAAGATGCTTTCAAATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATGCTTCTTGCTGTGTTGCCTATTTCCAGAAGACTACGACATCCCAATAGAAGACTTGTTCCAGTATTCAATTGGGAAAGGATTGTTTCGAGATACCGACACAATGTATGAAGCCAGAGGAATCGCACATTCAGTGATCAAGCACCTGAAAGATTCTAGCTTGCTGTTAGAGGGTGAATTCAGTGGACACAGTGGTGAATGTGTAAAGATGCATGATGTCGTCCGGGATACAGCCATGAGAATCGCAAAATGTGAAGATGGGCATGGGTTTTTGGTGAAAGCTGGATGTGGTTTAAATCATTGGTCATGTCGATCACATGAAGGCTACTCTGCAATCTCACTAATGGAGAATAAAATTCGCCGCCTACCCGAAGAGTTGGTAGGTCCAAAACTTCAGATTTTATTACTACAAGACAATACTGATTTAAATGAGATCCCAGAGACCTTTTTCCCAAGTCCCAATGAATTAAGGCTCTTGGATCTTAGCAAAACTATTATTTCCTCTCTACCCCAATCTTTCAGTCTCCTTATCAACCTCCAAGCTTTGTATTTAGATTTTTGCGACGAATTGATTGACATTTCTGTATTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGGAACTATCCTGGGGGGGAATTGTCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGGAATTCAGTGGCGCTTGGATTAGAGCTTTAAATTATACATGA

Protein Analysis

498

Amino Acids

56.46

Weight (kDa)

6.31

Isoelectric Point (pI)

43.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 14 - 176 6.8e-33 NB-ARC domain
WHD_DRP PF23559 264 - 333 8.3e-06 Disease resistance protein Winged helix domain
LRR_8 PF13855 386 - 444 2e-07 Leucine rich repeat
LRR_14 PF23598 396 - 487 1.3e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 278
AccI GTMKAC 2 cut(s) 99, 595
AciI CCGC 1 cut(s) 1132
AclWI GGATC 2 cut(s) 1198, 1257
AcsI RAATTY 9 cut(s) 222, 378, 409, 542, 947, 1125, 1380, 1432, 1457
AcuI CTGAAG 2 cut(s) 1148, 1388
AcyI GRCGYC 1 cut(s) 114
AfiI CCNNNNNNNGG 2 cut(s) 278, 1446
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 6 cut(s) 227, 542, 721, 751, 840, 1379
AhdI GACNNNNNGTC 1 cut(s) 766
AjnI CCWGG 1 cut(s) 1402
AjuI GAANNNNNNNTTGG 2 cut(s) 456, 488
AleI CACNNNNGTG 1 cut(s) 963
Alw26I GTCTC 3 cut(s) 482, 1205, 1298
AlwI GGATC 2 cut(s) 1198, 1257
AoxI GGCC 1 cut(s) 252
ApeKI GCWGC 2 cut(s) 146, 662
ApoI RAATTY 9 cut(s) 222, 378, 409, 542, 947, 1125, 1380, 1432, 1457
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 1469
AspS9I GGNCC 2 cut(s) 253, 1154
AsuC2I CCSGG 2 cut(s) 258, 995
AsuHPI GGTGA 5 cut(s) 145, 418, 956, 977, 1057
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 1154
BaeGI GKGCMC 1 cut(s) 628
BbsI GAAGAC 2 cut(s) 807, 828
BbvI GCAGC 2 cut(s) 133, 674
BccI CCATC 1 cut(s) 1022
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 2 cut(s) 460, 494
BciT130I CCWGG 1 cut(s) 1404
BciVI GTATCC 2 cut(s) 208, 991
BclI TGATCA 1 cut(s) 906
BcnI CCSGG 2 cut(s) 258, 995
BcoDI GTCTC 3 cut(s) 482, 1205, 1298
BfaI CTAG 4 cut(s) 291, 384, 432, 927
BfmI CTRYAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 1470
BfuI GTATCC 2 cut(s) 208, 991
BglII AGATCT 1 cut(s) 533
BisI GCNGC 3 cut(s) 147, 663, 1132
BlsI GCNGC 3 cut(s) 148, 664, 1133
Bme1390I CCNGG 3 cut(s) 258, 995, 1404
Bme18I GGWCC 1 cut(s) 1154
BmeRI GACNNNNNGTC 1 cut(s) 766
BmgT120I GGNCC 2 cut(s) 253, 1154
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 3 cut(s) 258, 995, 1404
BmsI GCATC 2 cut(s) 703, 969
BpiI GAAGAC 2 cut(s) 807, 828
Bpu14I TTCGAA 1 cut(s) 216
BpuMI CCSGG 2 cut(s) 258, 995
BsaHI GRCGYC 1 cut(s) 114
BsaI GGTCTC 1 cut(s) 1205
BsaJI CCNNGG 4 cut(s) 45, 129, 256, 1403
Bsc4I CCNNNNNNNGG 2 cut(s) 278, 1446
Bse1I ACTGG 2 cut(s) 255, 832
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 1 cut(s) 1404
BseDI CCNNGG 4 cut(s) 45, 129, 256, 1403
BseGI GGATG 3 cut(s) 324, 810, 1061
BseLI CCNNNNNNNGG 2 cut(s) 278, 1446
BseMI GCAATG 1 cut(s) 600
BseNI ACTGG 2 cut(s) 255, 832
BseSI GKGCMC 1 cut(s) 628
BseXI GCAGC 2 cut(s) 133, 674
BshFI GGCC 1 cut(s) 254
BsiSI CCGG 2 cut(s) 258, 994
BslFI GGGAC 2 cut(s) 181, 1212
BslI CCNNNNNNNGG 2 cut(s) 278, 1446
BsmAI GTCTC 3 cut(s) 482, 1205, 1298
BsmBI CGTCTC 1 cut(s) 482
BsmFI GGGAC 2 cut(s) 181, 1212
BsmI GAATGC 1 cut(s) 471
BsnI GGCC 1 cut(s) 254
Bso31I GGTCTC 1 cut(s) 1205
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 628
Bsp143I GATC 5 cut(s) 533, 906, 1082, 1203, 1249
Bsp19I CCATGG 2 cut(s) 45, 129
BspACI CCGC 1 cut(s) 1132
BspANI GGCC 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 255
BspMAI CTGCAG 1 cut(s) 151
BspPI GGATC 2 cut(s) 1198, 1257
BspQI GCTCTTC 1 cut(s) 277
BspT104I TTCGAA 1 cut(s) 216
BspTNI GGTCTC 1 cut(s) 1205
BsrDI GCAATG 1 cut(s) 600
BsrI ACTGG 2 cut(s) 255, 832
BssECI CCNNGG 4 cut(s) 45, 129, 256, 1403
BssMI GATC 5 cut(s) 533, 906, 1082, 1203, 1249
BssNI GRCGYC 1 cut(s) 114
BssT1I CCWWGG 2 cut(s) 45, 129
Bst2UI CCWGG 1 cut(s) 1404
Bst4CI ACNGT 2 cut(s) 88, 962
Bst6I CTCTTC 2 cut(s) 277, 1137
BstACI GRCGYC 1 cut(s) 114
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 932
BstDEI CTNAG 3 cut(s) 1253, 1385, 1445
BstDSI CCRYGG 2 cut(s) 45, 129
BstF5I GGATG 3 cut(s) 324, 810, 1061
BstH2I RGCGCY 1 cut(s) 1470
BstHHI GCGC 1 cut(s) 1469
BstKTI GATC 5 cut(s) 536, 909, 1085, 1206, 1252
BstMAI GTCTC 3 cut(s) 482, 1205, 1298
BstMBI GATC 5 cut(s) 533, 906, 1082, 1203, 1249
BstMWI GCNNNNNNNGC 3 cut(s) 111, 468, 611
BstNI CCWGG 1 cut(s) 1404
BstNSI RCATGY 2 cut(s) 143, 446
BstSCI CCNGG 3 cut(s) 256, 993, 1402
BstSFI CTRYAG 1 cut(s) 147
BstSLI GKGCMC 1 cut(s) 628
BstV1I GCAGC 2 cut(s) 133, 674
BstV2I GAAGAC 2 cut(s) 807, 828
BstX2I RGATCY 3 cut(s) 533, 1203, 1249
BstYI RGATCY 3 cut(s) 533, 1203, 1249
BsuI GTATCC 2 cut(s) 208, 991
BsuRI GGCC 1 cut(s) 254
BtgI CCRYGG 2 cut(s) 45, 129
BtsCI GGATG 3 cut(s) 324, 810, 1061
BtsIMutI CAGTG 5 cut(s) 84, 909, 958, 967, 1468
Cac8I GCNNGC 1 cut(s) 932
CfoI GCGC 1 cut(s) 1469
Cfr13I GGNCC 2 cut(s) 253, 1154
CseI GACGC 1 cut(s) 103
DdeI CTNAG 3 cut(s) 1253, 1385, 1445
DpnI GATC 5 cut(s) 535, 908, 1084, 1205, 1251
DpnII GATC 5 cut(s) 533, 906, 1082, 1203, 1249
DraI TTTAAA 4 cut(s) 28, 1065, 1197, 1485
DriI GACNNNNNGTC 1 cut(s) 766
Eam1104I CTCTTC 2 cut(s) 277, 1137
Eam1105I GACNNNNNGTC 1 cut(s) 766
EarI CTCTTC 2 cut(s) 277, 1137
Eco130I CCWWGG 2 cut(s) 45, 129
Eco31I GGTCTC 1 cut(s) 1205
Eco47I GGWCC 1 cut(s) 1154
Eco57I CTGAAG 2 cut(s) 1148, 1388
EcoRI GAATTC 6 cut(s) 222, 378, 409, 542, 947, 1457
EcoRII CCWGG 1 cut(s) 1402
EcoT14I CCWWGG 2 cut(s) 45, 129
EcoT22I ATGCAT 1 cut(s) 984
ErhI CCWWGG 2 cut(s) 45, 129
Esp3I CGTCTC 1 cut(s) 482
FalI AAGNNNNNCTT 2 cut(s) 758, 790
FaqI GGGAC 2 cut(s) 181, 1212
FbaI TGATCA 1 cut(s) 906
FblI GTMKAC 2 cut(s) 99, 595
Fnu4HI GCNGC 3 cut(s) 147, 663, 1132
FokI GGATG 3 cut(s) 311, 797, 1068
Fsp4HI GCNGC 3 cut(s) 147, 663, 1132
FspBI CTAG 4 cut(s) 291, 384, 432, 927
GlaI GCGC 1 cut(s) 1468
GluI GCNGC 3 cut(s) 147, 663, 1132
HaeII RGCGCY 1 cut(s) 1470
HaeIII GGCC 1 cut(s) 254
HapII CCGG 2 cut(s) 258, 994
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 1469
Hin1I GRCGYC 1 cut(s) 114
Hin6I GCGC 1 cut(s) 1467
HinP1I GCGC 1 cut(s) 1467
HincII GTYRAC 1 cut(s) 369
HindII GTYRAC 1 cut(s) 369
HindIII AAGCTT 2 cut(s) 1311, 1373
HinfI GANTC 5 cut(s) 152, 500, 891, 923, 1011
HpaII CCGG 2 cut(s) 258, 994
HphI GGTGA 5 cut(s) 145, 418, 956, 977, 1057
Hpy166II GTNNAC 4 cut(s) 100, 369, 596, 956
Hpy188I TCNGA 2 cut(s) 493, 1167
Hpy188III TCNNGA 5 cut(s) 291, 479, 797, 860, 1419
Hpy8I GTNNAC 4 cut(s) 100, 369, 596, 956
Hpy99I CGWCG 2 cut(s) 119, 1337
HpyAV CCTTC 3 cut(s) 55, 565, 1085
HpyCH4III ACNGT 2 cut(s) 88, 962
HpyCH4V TGCA 7 cut(s) 149, 159, 442, 605, 620, 982, 1103
HpyF10VI GCNNNNNNNGC 3 cut(s) 111, 468, 611
HpyF3I CTNAG 3 cut(s) 1253, 1385, 1445
Hsp92I GRCGYC 1 cut(s) 114
HspAI GCGC 1 cut(s) 1467
Ksp22I TGATCA 1 cut(s) 906
Kzo9I GATC 5 cut(s) 533, 906, 1082, 1203, 1249
LguI GCTCTTC 1 cut(s) 277
LmnI GCTCC 3 cut(s) 64, 244, 507
Lsp1109I GCAGC 2 cut(s) 133, 674
LweI GCATC 2 cut(s) 703, 969
MaeI CTAG 4 cut(s) 291, 384, 432, 927
MaeIII GTNAC 2 cut(s) 82, 267
MalI GATC 5 cut(s) 535, 908, 1084, 1205, 1251
MboI GATC 5 cut(s) 533, 906, 1082, 1203, 1249
MboII GAAGA 9 cut(s) 294, 532, 719, 722, 812, 833, 1037, 1154, 1381
MfeI CAATTG 1 cut(s) 840
MflI RGATCY 3 cut(s) 533, 1203, 1249
MhlI GDGCHC 1 cut(s) 628
Mph1103I ATGCAT 1 cut(s) 984
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 7 cut(s) 27, 174, 734, 1064, 1196, 1238, 1484
MslI CAYNNNNRTG 1 cut(s) 963
MspI CCGG 2 cut(s) 258, 994
MspR9I CCNGG 3 cut(s) 258, 995, 1404
MunI CAATTG 1 cut(s) 840
Mva1269I GAATGC 1 cut(s) 471
MvaI CCWGG 1 cut(s) 1404
MwoI GCNNNNNNNGC 3 cut(s) 111, 468, 611
NciI CCSGG 2 cut(s) 258, 995
NcoI CCATGG 2 cut(s) 45, 129
NdeII GATC 5 cut(s) 533, 906, 1082, 1203, 1249
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 1 cut(s) 82
NsiI ATGCAT 1 cut(s) 984
NspI RCATGY 2 cut(s) 143, 446
NspV TTCGAA 1 cut(s) 216
OliI CACNNNNGTG 1 cut(s) 963
PciI ACATGT 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 277
PctI GAATGC 1 cut(s) 471
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 5 cut(s) 152, 500, 891, 923, 1011
PflFI GACNNNGTC 1 cut(s) 80
PflMI CCANNNNNTGG 1 cut(s) 278
PfoI TCCNGGA 1 cut(s) 993
PkrI GCNGC 3 cut(s) 148, 664, 1133
PscI ACATGT 1 cut(s) 139
Psp6I CCWGG 1 cut(s) 1402
PspGI CCWGG 1 cut(s) 1402
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 253, 1154
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 3 cut(s) 533, 1203, 1249
PsyI GACNNNGTC 1 cut(s) 80
RseI CAYNNNNRTG 1 cut(s) 963
SapI GCTCTTC 1 cut(s) 277
SaqAI TTAA 7 cut(s) 27, 174, 734, 1064, 1196, 1238, 1484
SatI GCNGC 3 cut(s) 147, 663, 1132
Sau3AI GATC 5 cut(s) 533, 906, 1082, 1203, 1249
Sau96I GGNCC 2 cut(s) 253, 1154
ScrFI CCNGG 3 cut(s) 258, 995, 1404
SduI GDGCHC 1 cut(s) 628
SfaNI GCATC 2 cut(s) 703, 969
SfcI CTRYAG 1 cut(s) 147
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 1 cut(s) 1154
SmiI ATTTAAAT 1 cut(s) 1197
SmiMI CAYNNNNRTG 1 cut(s) 963
SsiI CCGC 1 cut(s) 1132
SspMI CTAG 4 cut(s) 291, 384, 432, 927
StyD4I CCNGG 3 cut(s) 256, 993, 1402
StyI CCWWGG 2 cut(s) 45, 129
SwaI ATTTAAAT 1 cut(s) 1197
TaaI ACNGT 2 cut(s) 88, 962
TaqI TCGA 6 cut(s) 216, 264, 480, 859, 1081, 1418
TauI GCSGC 1 cut(s) 1134
TfiI GAWTC 5 cut(s) 152, 500, 891, 923, 1011
Tru1I TTAA 7 cut(s) 27, 174, 734, 1064, 1196, 1238, 1484
Tru9I TTAA 7 cut(s) 27, 174, 734, 1064, 1196, 1238, 1484
TscAI CASTG 5 cut(s) 91, 909, 958, 967, 1468
TseFI GTSAC 1 cut(s) 82
TseI GCWGC 2 cut(s) 146, 662
Tsp45I GTSAC 1 cut(s) 82
TspDTI ATGAA 5 cut(s) 74, 720, 894, 1104, 1248
TspGWI ACGGA 1 cut(s) 24
TspRI CASTG 5 cut(s) 91, 909, 958, 967, 1468
Tth111I GACNNNGTC 1 cut(s) 80
Van91I CCANNNNNTGG 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 1154
XapI RAATTY 9 cut(s) 222, 378, 409, 542, 947, 1125, 1380, 1432, 1457
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 2 cut(s) 143, 446
XcmI CCANNNNNNNNNTGG 1 cut(s) 839
XmiI GTMKAC 2 cut(s) 99, 595
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 4 cut(s) 291, 384, 432, 927
Zsp2I ATGCAT 1 cut(s) 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.