Rmu_sc0002187.1_g000037

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002187.1
OG0000379
Physical Location & Seq
Reverse (-)
145390 .. 149319
3930 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002187.1_g000037.1.cds

Sequence Viewer

Length: 3447 bp
atgtcgttggcgattattgtggtaattgtgacatggatcggtgccatagctactattaagtggatatggactcttctgaagcattggttcggttacatggttgaaaggcgcgagccaattggcatcgctactgagtggaagtatgatgtgtttttgagtttcaggggtcctgacactcgcaaggggattacagtcgacatacaggatcgacttaataggagtggaattaaaacgttcatggatgatcgagatcttgaagtagggaatgttattagtcccgctctcttaacagcaatcagagaatcaaagtttgcaatcgttgttctctcgcaaaattatgctttttctacttggtgtttggaggaacttagagagatttgtctctgcatggaagacaacagaattttgccacttttttatgatgttgatcctgcttatgttcgatatcagaacgggagatacggagatgctttctctaagcatgaaacctctgggcgacatgaatcaaagaaggtgaagcaatggagagctgctttaaaaaaagtggccgacatttctgggtggaatacaaatgattataagactcacaaagaacttgttgacatcattgtggaatctctctgcagtaaagtaccacctgatgcaattgaatccacaggagatttccaagcatatgaagcaacaacagaagccatgaataaggttatgaaggcgctaacagatgacgaggtcagtgccgttggtgtctacggcatgggcggcgttggaaagacaagcatggcgagacatgtagctgcaaaagcctgcaaaaacggtacttttaaccgttggattatagttgccatatcccaaagccccgacttgagaaaaattcaagacacattggcagatttcttgggcgttaaattggacaaggagacagaagatggaagagcggctgcgctgcataaggagataatgagaagaggaagactcctgataatcttggacgatgtttggaacagaatagaattgtcagccataggaattcccagctacgaggagcttcaaaagtgcaattccaaagtcgtactcaccacacggatattgcatgtttgtaattccatggacagccgagaaaacattcctctcaatatcttatcaaaacaagattcttggaaattgtttctgagaaatgcaggggtagcgaagaagaagattcttgaatcgaccacttttgaggatgtggcaagaagagtagccagagaatgcaggggtctacccattgcattgatagcagttgcaagggcactcggagatgaggaccaggaggaatgggaaaatgcagctaaacaactagaggagtcgcgatatgccaatcctaaccatgaggatgaaaatgcattcaattgtatcagattaagctatgattacttgaaaagtgaagaccgcaagtcgtgcttcttgctctgttgcctatacccagaagaccatgaaatcaaaatggaagacttgttcaggtatgcgatcggaaaaggattgtttcaagatgccgaaacaatggtaaaagccagaggaaaagtagtttcagtggtcaagtacctgaaagattctagcttgcttatggatggcgagaaaaaaggatgtgtaaagatgcatgatgtcatccgggatacagccattcaaattgccgaatccgggttttgggtgaaagctggctgtggtttaaagggttggccacgcggattacatgaaggctgcactgctatttcactgatgaggaacgaaattcgcaagctacccgaagaagagttggtatgtccaaatctccagattttattactaaaccggaatgctgatttagatgagatcccagaaaagtccatccaaaagctgaatgaattaaggctcttggatcttagcgacactagtatttccgtgttaccgcagtcattcagtctcctgaccaacctccaagctttgtatttagattctcccaagaaatttattgacatttctatagtgggaaaactgaagcttgaaattcttagtatgagagaatgtcccgggagcgggggattttcgagaccagcacatgaatatcccgaggagagggaattgccgagagaaataggacatttgaccaatctaaggattttggatgtcaatcgcagttgtgatttggccggcgtgagtggtatggtcacaattccatctaaagtgatatcaaagttgcataaattagaagaattgtacatggtgtactgtggattagaagaagaaaccaatatttttgatgagttagctggtttatcaaatttgaaaatagtgcaggttggcatatctgatgcaaaatacatccctaaaaatgttgaggccaaaccagattgggattacttttatataagtatcatcggcggacgcaactccggtgcatacagaagcggagatcataattcaagatccttgtatcttagagaagcaaccataagtgccttgcctgattggtttatcaacgcggtgacaaagaaaacggagacgctagagtatgattgctgcaaagggatgagtgacattcttatagaatatgaccatgggatgttacataaactcaagcatctcacagtagctggtcaaattcttcattcctatgtgtacttgaaagagttgatgaacacaacaagacgagttcaaaaaggaccagtgtttgagaatttggaagagttgcgtctgttagcactgatccaccttgaggagttgtgtgttggtgatttaccacttgggtctttctccaatctgaagagattgcatatgtttggttgtttaatcttgaagaatgtatcaaaattggtacaaagactaccaaatctggagaaactagatctaaattggatgcctaaattggaatatgtgtttggatgggaagggtttgagctgaaacaatcaaaactgagagagatgcatttgttgggtccaaattcagtgaagagcatttgtagaggtcctgcctcacgcataatgtttcagagtcttaaaagtttgttcttttaccgttgcgagttgctgcaaagtctgttcgcatgtgatgtagccgagtgtcttgttcaattggaagacctttttgtagagcggtgctatttgttgaaaagagtaattgaagcagtgaacaatgagaagacggttctaccaaaattgaagaacctggttttgaagaatcttcctatgttgtatggtgcaagtgctactgttgatattgagtgtccttcactggagcacttgattgtggtggattgcccccagtttccattttcaacttcttccgatctcttcgaatccttcaaaagcaggaaccatatttcattttcaacccctgcttctgactactttggcagcacgaacccagtcaaactcaatgatcgacaactgtacagcttcctatatgatagaacttctggtttccaattgtga

Protein Analysis

1148

Amino Acids

130.46

Weight (kDa)

6.37

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 579
Acc36I ACCTGC 1 cut(s) 2302
AccB1I GGYRCC 1 cut(s) 41
AccBSI CCGCTC 4 cut(s) 281, 935, 2052, 3109
AccI GTMKAC 3 cut(s) 195, 747, 1258
AccII CGCG 4 cut(s) 111, 1348, 1722, 2498
AclI AACGTT 1 cut(s) 233
AclWI GGATC 7 cut(s) 44, 213, 422, 1843, 1902, 2436, 2716
AcoI YGGCCR 3 cut(s) 546, 1715, 2163
AcuI CTGAAG 3 cut(s) 98, 2033, 2798
AfaI GTAC 9 cut(s) 633, 817, 1071, 1580, 2234, 2243, 2636, 2832, 3407
AfiI CCNNNNNNNGG 7 cut(s) 1677, 1683, 2051, 2052, 2091, 2130, 2731
AflIII ACRYGT 1 cut(s) 787
AhdI GACNNNNNGTC 1 cut(s) 1432
AhlI ACTAGT 1 cut(s) 1907
AjnI CCWGG 2 cut(s) 1305, 3180
AjuI GAANNNNNNNTTGG 2 cut(s) 2877, 2909
AloI GAACNNNNNNTCC 2 cut(s) 1478, 1510
Alw21I GWGCWC 1 cut(s) 3255
Alw26I GTCTC 6 cut(s) 386, 778, 911, 1943, 2059, 2510
AlwI GGATC 7 cut(s) 44, 213, 422, 1843, 1902, 2436, 2716
AlwNI CAGNNNCTG 1 cut(s) 2609
Ama87I CYCGRG 2 cut(s) 2045, 2084
AoxI GGCC 4 cut(s) 546, 1715, 2163, 2355
ApeKI GCWGC 9 cut(s) 530, 794, 938, 943, 1325, 1737, 2535, 3043, 3369
Asp700I GAANNNNTTC 1 cut(s) 1122
AspLEI GCGC 3 cut(s) 111, 715, 943
AspS9I GGNCC 5 cut(s) 167, 1303, 2678, 2951, 2981
AsuC2I CCSGG 4 cut(s) 1649, 1678, 2046, 2047
AsuHPI GGTGA 5 cut(s) 526, 1066, 1699, 2512, 2759
AsuII TTCGAA 1 cut(s) 3309
AvaI CYCGRG 2 cut(s) 2045, 2084
AvaII GGWCC 5 cut(s) 167, 1303, 2678, 2951, 2981
BaeGI GKGCMC 1 cut(s) 1291
BalI TGGCCA 1 cut(s) 1717
BanI GGYRCC 1 cut(s) 41
BbsI GAAGAC 7 cut(s) 399, 976, 1431, 1473, 1494, 3099, 3161
Bbv12I GWGCWC 1 cut(s) 3255
BbvI GCAGC 9 cut(s) 517, 781, 925, 930, 1337, 1724, 2522, 3030, 3381
BccI CCATC 5 cut(s) 920, 1601, 1871, 2200, 2892
BceAI ACGGC 2 cut(s) 722, 766
BcgI CGANNNNNNTGC 2 cut(s) 716, 750
BciT130I CCWGG 2 cut(s) 1307, 3182
BciVI GTATCC 1 cut(s) 1645
BcnI CCSGG 4 cut(s) 1649, 1678, 2046, 2047
BcoDI GTCTC 6 cut(s) 386, 778, 911, 1943, 2059, 2510
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 5 cut(s) 1337, 1593, 1908, 2522, 2858
BfmI CTRYAG 2 cut(s) 622, 1998
BfoI RGCGCY 1 cut(s) 716
BfuAI ACCTGC 1 cut(s) 2302
BfuI GTATCC 1 cut(s) 1645
BglII AGATCT 2 cut(s) 250, 2860
Bme1390I CCNGG 6 cut(s) 1307, 1649, 1678, 2046, 2047, 3182
Bme18I GGWCC 5 cut(s) 167, 1303, 2678, 2951, 2981
BmeRI GACNNNNNGTC 1 cut(s) 1432
BmeT110I CYCGRG 2 cut(s) 2045, 2084
BmgT120I GGNCC 5 cut(s) 167, 1303, 2678, 2951, 2981
BmiI GGNNCC 4 cut(s) 43, 168, 2952, 3329
BmrFI CCNGG 6 cut(s) 1307, 1649, 1678, 2046, 2047, 3182
BmrI ACTGGG 2 cut(s) 3271, 3374
BmsI GCATC 9 cut(s) 132, 457, 631, 1519, 1623, 2317, 2605, 2862, 2928
BmuI ACTGGG 2 cut(s) 3271, 3374
BpiI GAAGAC 7 cut(s) 399, 976, 1431, 1473, 1494, 3099, 3161
BplI GAGNNNNNCTC 2 cut(s) 957, 989
BpmI CTGGAG 3 cut(s) 1793, 2870, 3269
Bpu14I TTCGAA 1 cut(s) 3309
BpuEI CTTGAG 3 cut(s) 883, 2576, 2750
BpuMI CCSGG 4 cut(s) 1649, 1678, 2046, 2047
BsaBI GATNNNNATC 3 cut(s) 249, 426, 2145
BsaI GGTCTC 1 cut(s) 2059
BsaJI CCNNGG 4 cut(s) 1104, 2045, 2085, 2572
BsaWI WCCGGW 2 cut(s) 1827, 2408
Bsc4I CCNNNNNNNGG 7 cut(s) 1677, 1683, 2051, 2052, 2091, 2130, 2731
Bse118I RCCGGY 1 cut(s) 2165
Bse1I ACTGG 4 cut(s) 2681, 3252, 3277, 3380
Bse3DI GCAATG 2 cut(s) 527, 1263
Bse8I GATNNNNATC 3 cut(s) 249, 426, 2145
BseBI CCWGG 2 cut(s) 1307, 3182
BseDI CCNNGG 4 cut(s) 1104, 2045, 2085, 2572
BseJI GATNNNNATC 3 cut(s) 249, 426, 2145
BseLI CCNNNNNNNGG 7 cut(s) 1677, 1683, 2051, 2052, 2091, 2130, 2731
BseMI GCAATG 2 cut(s) 527, 1263
BseMII CTCAG 3 cut(s) 123, 1160, 2921
BseNI ACTGG 4 cut(s) 2681, 3252, 3277, 3380
BseRI GAGGAG 4 cut(s) 1055, 1355, 2102, 2747
BseSI GKGCMC 1 cut(s) 1291
BseXI GCAGC 9 cut(s) 517, 781, 925, 930, 1337, 1724, 2522, 3030, 3381
BseYI CCCAGC 1 cut(s) 1031
BsgI GTGCAG 2 cut(s) 1723, 2330
Bsh1236I CGCG 4 cut(s) 111, 1348, 1722, 2498
Bsh1285I CGRYCG 1 cut(s) 1509
BshFI GGCC 4 cut(s) 548, 1717, 2165, 2357
BshNI GGYRCC 1 cut(s) 41
BsiEI CGRYCG 1 cut(s) 1509
BsiHKAI GWGCWC 1 cut(s) 3255
BsiHKCI CYCGRG 2 cut(s) 2045, 2084
BsiSI CCGG 6 cut(s) 1648, 1677, 1828, 2046, 2166, 2409
BslFI GGGAC 2 cut(s) 261, 2028
BslI CCNNNNNNNGG 7 cut(s) 1677, 1683, 2051, 2052, 2091, 2130, 2731
BsmAI GTCTC 6 cut(s) 386, 778, 911, 1943, 2059, 2510
BsmBI CGTCTC 1 cut(s) 2510
BsmFI GGGAC 2 cut(s) 261, 2028
BsmI GAATGC 3 cut(s) 1253, 1382, 1837
BsnI GGCC 4 cut(s) 548, 1717, 2165, 2357
Bso31I GGTCTC 1 cut(s) 2059
BsoBI CYCGRG 2 cut(s) 2045, 2084
Bsp119I TTCGAA 1 cut(s) 3309
Bsp1286I GDGCHC 2 cut(s) 1291, 3255
Bsp1407I TGTACA 2 cut(s) 2232, 3405
Bsp19I CCATGG 2 cut(s) 1104, 2572
Bsp68I TCGCGA 1 cut(s) 1348
BspANI GGCC 4 cut(s) 548, 1717, 2165, 2357
BspCNI CTCAG 3 cut(s) 124, 1161, 2922
BspFNI CGCG 4 cut(s) 111, 1348, 1722, 2498
BspLI GGNNCC 4 cut(s) 43, 168, 2952, 3329
BspMAI CTGCAG 1 cut(s) 626
BspMI ACCTGC 1 cut(s) 2302
BspPI GGATC 7 cut(s) 44, 213, 422, 1843, 1902, 2436, 2716
BspQI GCTCTTC 2 cut(s) 925, 2960
BspT104I TTCGAA 1 cut(s) 3309
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 2059
BsrBI CCGCTC 4 cut(s) 281, 935, 2052, 3109
BsrDI GCAATG 2 cut(s) 527, 1263
BsrFI RCCGGY 1 cut(s) 2165
BsrGI TGTACA 2 cut(s) 2232, 3405
BsrI ACTGG 4 cut(s) 2681, 3252, 3277, 3380
BssAI RCCGGY 1 cut(s) 2165
BssECI CCNNGG 4 cut(s) 1104, 2045, 2085, 2572
BssT1I CCWWGG 2 cut(s) 1104, 2572
Bst2UI CCWGG 2 cut(s) 1307, 3182
Bst4CI ACNGT 9 cut(s) 193, 815, 827, 2246, 2605, 3032, 3160, 3226, 3405
Bst6I CTCTTC 9 cut(s) 78, 925, 958, 1228, 1782, 2694, 2774, 2960, 3311
BstAPI GCANNNNNTGC 1 cut(s) 1437
BstAUI TGTACA 2 cut(s) 2232, 3405
BstBI TTCGAA 1 cut(s) 3309
BstC8I GCNNGC 6 cut(s) 113, 805, 1598, 1696, 1775, 2167
BstDEI CTNAG 9 cut(s) 132, 368, 477, 1169, 1898, 2027, 2129, 2453, 2930
BstDSI CCRYGG 2 cut(s) 1104, 2572
BstFNI CGCG 4 cut(s) 111, 1348, 1722, 2498
BstH2I RGCGCY 1 cut(s) 716
BstHHI GCGC 3 cut(s) 111, 715, 943
BstMAI GTCTC 6 cut(s) 386, 778, 911, 1943, 2059, 2510
BstMCI CGRYCG 1 cut(s) 1509
BstMWI GCNNNNNNNGC 6 cut(s) 677, 759, 800, 1184, 1274, 1437
BstNI CCWGG 2 cut(s) 1307, 3182
BstNSI RCATGY 3 cut(s) 791, 1094, 3063
BstSCI CCNGG 6 cut(s) 1305, 1647, 1676, 2044, 2045, 3180
BstSFI CTRYAG 2 cut(s) 622, 1998
BstSLI GKGCMC 1 cut(s) 1291
BstUI CGCG 4 cut(s) 111, 1348, 1722, 2498
BstV1I GCAGC 9 cut(s) 517, 781, 925, 930, 1337, 1724, 2522, 3030, 3381
BstV2I GAAGAC 7 cut(s) 399, 976, 1431, 1473, 1494, 3099, 3161
BstX2I RGATCY 5 cut(s) 250, 1848, 1894, 2441, 2860
BstYI RGATCY 5 cut(s) 250, 1848, 1894, 2441, 2860
BsuI GTATCC 1 cut(s) 1645
BsuRI GGCC 4 cut(s) 548, 1717, 2165, 2357
BtgI CCRYGG 2 cut(s) 1104, 2572
BtgZI GCGATG 1 cut(s) 109
BtsI GCAGTG 2 cut(s) 1740, 3147
BtsIMutI CAGTG 9 cut(s) 739, 1575, 1740, 1751, 2688, 2717, 2967, 3147, 3245
BtuMI TCGCGA 1 cut(s) 1348
BveI ACCTGC 1 cut(s) 2302
Cac8I GCNNGC 6 cut(s) 113, 805, 1598, 1696, 1775, 2167
CaiI CAGNNNCTG 1 cut(s) 2609
CfoI GCGC 3 cut(s) 111, 715, 943
Cfr10I RCCGGY 1 cut(s) 2165
Cfr13I GGNCC 5 cut(s) 167, 1303, 2678, 2951, 2981
Cfr9I CCCGGG 1 cut(s) 2045
CseI GACGC 3 cut(s) 2409, 2527, 2696
CsiI ACCWGGT 1 cut(s) 3180
Csp6I GTAC 9 cut(s) 632, 816, 1070, 1579, 2233, 2242, 2635, 2831, 3406
CviQI GTAC 9 cut(s) 632, 816, 1070, 1579, 2233, 2242, 2635, 2831, 3406
DdeI CTNAG 9 cut(s) 132, 368, 477, 1169, 1898, 2027, 2129, 2453, 2930
DraI TTTAAA 2 cut(s) 537, 1707
DriI GACNNNNNGTC 1 cut(s) 1432
EaeI YGGCCR 3 cut(s) 546, 1715, 2163
Eam1104I CTCTTC 9 cut(s) 78, 925, 958, 1228, 1782, 2694, 2774, 2960, 3311
Eam1105I GACNNNNNGTC 1 cut(s) 1432
EarI CTCTTC 9 cut(s) 78, 925, 958, 1228, 1782, 2694, 2774, 2960, 3311
EciI GGCGGA 1 cut(s) 2412
Eco130I CCWWGG 2 cut(s) 1104, 2572
Eco31I GGTCTC 1 cut(s) 2059
Eco32I GATATC 2 cut(s) 446, 2205
Eco47I GGWCC 5 cut(s) 167, 1303, 2678, 2951, 2981
Eco57I CTGAAG 3 cut(s) 98, 2033, 2798
Eco88I CYCGRG 2 cut(s) 2045, 2084
EcoO109I RGGNCCY 2 cut(s) 167, 2981
EcoRI GAATTC 1 cut(s) 1026
EcoRII CCWGG 2 cut(s) 1305, 3180
EcoRV GATATC 2 cut(s) 446, 2205
EcoT14I CCWWGG 2 cut(s) 1104, 2572
EcoT22I ATGCAT 3 cut(s) 1384, 1638, 2943
ErhI CCWWGG 2 cut(s) 1104, 2572
Esp3I CGTCTC 1 cut(s) 2510
FalI AAGNNNNNCTT 2 cut(s) 1424, 1456
FaqI GGGAC 2 cut(s) 261, 2028
FauI CCCGC 2 cut(s) 286, 2045
FauNDI CATATG 2 cut(s) 673, 2790
FblI GTMKAC 3 cut(s) 195, 747, 1258
FspBI CTAG 5 cut(s) 1337, 1593, 1908, 2522, 2858
GlaI GCGC 3 cut(s) 110, 714, 942
GsaI CCCAGC 1 cut(s) 1035
GsuI CTGGAG 3 cut(s) 1793, 2870, 3269
HaeII RGCGCY 1 cut(s) 716
HaeIII GGCC 4 cut(s) 548, 1717, 2165, 2357
HapII CCGG 6 cut(s) 1648, 1677, 1828, 2046, 2166, 2409
HgaI GACGC 3 cut(s) 2409, 2527, 2696
HhaI GCGC 3 cut(s) 111, 715, 943
Hin6I GCGC 3 cut(s) 109, 713, 941
HinP1I GCGC 3 cut(s) 109, 713, 941
HincII GTYRAC 2 cut(s) 196, 601
HindII GTYRAC 2 cut(s) 196, 601
HindIII AAGCTT 2 cut(s) 1956, 2015
HpaII CCGG 6 cut(s) 1648, 1677, 1828, 2046, 2166, 2409
HphI GGTGA 5 cut(s) 526, 1066, 1699, 2512, 2759
Hpy166II GTNNAC 7 cut(s) 196, 601, 748, 1259, 2242, 2635, 3145
Hpy8I GTNNAC 7 cut(s) 196, 601, 748, 1259, 2242, 2635, 3145
HpyAV CCTTC 6 cut(s) 505, 703, 1727, 2897, 3252, 3325
HpyCH4III ACNGT 9 cut(s) 193, 815, 827, 2246, 2605, 3032, 3160, 3226, 3405
HpyCH4IV ACGT 1 cut(s) 233
HpyF10VI GCNNNNNNNGC 6 cut(s) 677, 759, 800, 1184, 1274, 1437
HpyF3I CTNAG 9 cut(s) 132, 368, 477, 1169, 1898, 2027, 2129, 2453, 2930
HpySE526I ACGT 1 cut(s) 233
HspAI GCGC 3 cut(s) 109, 713, 941
KroI GCCGGC 1 cut(s) 2165
KroNI GCCGGC 1 cut(s) 2167
LguI GCTCTTC 2 cut(s) 925, 2960
LmnI GCTCC 3 cut(s) 1042, 2049, 3250
Lsp1109I GCAGC 9 cut(s) 517, 781, 925, 930, 1337, 1724, 2522, 3030, 3381
LweI GCATC 9 cut(s) 132, 457, 631, 1519, 1623, 2317, 2605, 2862, 2928
MabI ACCWGGT 1 cut(s) 3180
MaeI CTAG 5 cut(s) 1337, 1593, 1908, 2522, 2858
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 7 cut(s) 28, 92, 1920, 2182, 2500, 2549, 2580
MbiI CCGCTC 4 cut(s) 281, 935, 2052, 3109
MfeI CAATTG 5 cut(s) 117, 645, 1387, 3086, 3440
MflI RGATCY 5 cut(s) 250, 1848, 1894, 2441, 2860
MhlI GDGCHC 2 cut(s) 1291, 3255
MlsI TGGCCA 1 cut(s) 1717
MluNI TGGCCA 1 cut(s) 1717
MlyI GAGTC 5 cut(s) 64, 577, 966, 1352, 3016
MmeI TCCRAC 2 cut(s) 745, 809
Mox20I TGGCCA 1 cut(s) 1717
Mph1103I ATGCAT 3 cut(s) 1384, 1638, 2943
MroNI GCCGGC 1 cut(s) 2165
MroXI GAANNNNTTC 1 cut(s) 1122
MscI TGGCCA 1 cut(s) 1717
MslI CAYNNNNRTG 3 cut(s) 608, 1371, 2628
Msp20I TGGCCA 1 cut(s) 1717
MspI CCGG 6 cut(s) 1648, 1677, 1828, 2046, 2166, 2409
MspR9I CCNGG 6 cut(s) 1307, 1649, 1678, 2046, 2047, 3182
MunI CAATTG 5 cut(s) 117, 645, 1387, 3086, 3440
Mva1269I GAATGC 3 cut(s) 1253, 1382, 1837
MvaI CCWGG 2 cut(s) 1307, 3182
MvnI CGCG 4 cut(s) 111, 1348, 1722, 2498
MwoI GCNNNNNNNGC 6 cut(s) 677, 759, 800, 1184, 1274, 1437
NaeI GCCGGC 1 cut(s) 2167
NciI CCSGG 4 cut(s) 1649, 1678, 2046, 2047
NcoI CCATGG 2 cut(s) 1104, 2572
NdeI CATATG 2 cut(s) 673, 2790
NgoMIV GCCGGC 1 cut(s) 2165
NlaIV GGNNCC 4 cut(s) 43, 168, 2952, 3329
NmeAIII GCCGAG 3 cut(s) 1139, 2127, 3097
NmuCI GTSAC 4 cut(s) 28, 2182, 2500, 2549
NruI TCGCGA 1 cut(s) 1348
NsiI ATGCAT 3 cut(s) 1384, 1638, 2943
NspI RCATGY 3 cut(s) 791, 1094, 3063
NspV TTCGAA 1 cut(s) 3309
PciI ACATGT 1 cut(s) 787
PciSI GCTCTTC 2 cut(s) 925, 2960
PctI GAATGC 3 cut(s) 1253, 1382, 1837
PdiI GCCGGC 1 cut(s) 2167
PdmI GAANNNNTTC 1 cut(s) 1122
PflFI GACNNNGTC 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 1647
Ple19I CGATCG 1 cut(s) 1509
PleI GAGTC 5 cut(s) 64, 577, 966, 1351, 3015
PpsI GAGTC 5 cut(s) 64, 577, 966, 1351, 3015
PpuMI RGGWCCY 2 cut(s) 167, 2981
PscI ACATGT 1 cut(s) 787
PsiI TTATAA 1 cut(s) 579
Psp1406I AACGTT 1 cut(s) 233
Psp5II RGGWCCY 2 cut(s) 167, 2981
Psp6I CCWGG 2 cut(s) 1305, 3180
PspFI CCCAGC 1 cut(s) 1031
PspGI CCWGG 2 cut(s) 1305, 3180
PspN4I GGNNCC 4 cut(s) 43, 168, 2952, 3329
PspPI GGNCC 5 cut(s) 167, 1303, 2678, 2951, 2981
PspPPI RGGWCCY 2 cut(s) 167, 2981
PsrI GAACNNNNNNTAC 2 cut(s) 443, 475
PstI CTGCAG 1 cut(s) 626
PstNI CAGNNNCTG 1 cut(s) 2609
PsuI RGATCY 5 cut(s) 250, 1848, 1894, 2441, 2860
PsyI GACNNNGTC 1 cut(s) 728
PvuI CGATCG 1 cut(s) 1509
RruI TCGCGA 1 cut(s) 1348
RsaI GTAC 9 cut(s) 633, 817, 1071, 1580, 2234, 2243, 2636, 2832, 3407
RsaNI GTAC 9 cut(s) 632, 816, 1070, 1579, 2233, 2242, 2635, 2831, 3406
RseI CAYNNNNRTG 3 cut(s) 608, 1371, 2628
SalI GTCGAC 1 cut(s) 194
SapI GCTCTTC 2 cut(s) 925, 2960
Sau96I GGNCC 5 cut(s) 167, 1303, 2678, 2951, 2981
SchI GAGTC 5 cut(s) 64, 577, 966, 1352, 3016
ScrFI CCNGG 6 cut(s) 1307, 1649, 1678, 2046, 2047, 3182
SduI GDGCHC 2 cut(s) 1291, 3255
SexAI ACCWGGT 1 cut(s) 3180
SfaNI GCATC 9 cut(s) 132, 457, 631, 1519, 1623, 2317, 2605, 2862, 2928
SfcI CTRYAG 2 cut(s) 622, 1998
SfuI TTCGAA 1 cut(s) 3309
SinI GGWCC 5 cut(s) 167, 1303, 2678, 2951, 2981
SmaI CCCGGG 1 cut(s) 2047
SmiMI CAYNNNNRTG 3 cut(s) 608, 1371, 2628
SmlI CTYRAG 3 cut(s) 862, 2591, 2729
SmoI CTYRAG 3 cut(s) 862, 2591, 2729
SpeI ACTAGT 1 cut(s) 1907
SspI AATATT 1 cut(s) 2269
SspMI CTAG 5 cut(s) 1337, 1593, 1908, 2522, 2858
StyD4I CCNGG 6 cut(s) 1305, 1647, 1676, 2044, 2045, 3180
StyI CCWWGG 2 cut(s) 1104, 2572
TaaI ACNGT 9 cut(s) 193, 815, 827, 2246, 2605, 3032, 3160, 3226, 3405
TaiI ACGT 1 cut(s) 236
TaqI TCGA 8 cut(s) 195, 208, 247, 442, 1208, 2063, 3309, 3397
TatI WGTACW 4 cut(s) 2232, 2241, 2634, 3405
TauI GCSGC 2 cut(s) 762, 938
TscAI CASTG 9 cut(s) 739, 1575, 1747, 1758, 2688, 2724, 2967, 3147, 3252
TseFI GTSAC 4 cut(s) 28, 2182, 2500, 2549
TseI GCWGC 9 cut(s) 530, 794, 938, 943, 1325, 1737, 2535, 3043, 3369
Tsp45I GTSAC 4 cut(s) 28, 2182, 2500, 2549
TspGWI ACGGA 4 cut(s) 477, 1096, 1906, 2528
TspMI CCCGGG 1 cut(s) 2045
TspRI CASTG 9 cut(s) 739, 1575, 1747, 1758, 2688, 2724, 2967, 3147, 3252
Tth111I GACNNNGTC 1 cut(s) 728
VpaK11BI GGWCC 5 cut(s) 167, 1303, 2678, 2951, 2981
XceI RCATGY 3 cut(s) 791, 1094, 3063
XmaI CCCGGG 1 cut(s) 2045
XmiI GTMKAC 3 cut(s) 195, 747, 1258
XmnI GAANNNNTTC 1 cut(s) 1122
XspI CTAG 5 cut(s) 1337, 1593, 1908, 2522, 2858
Zsp2I ATGCAT 3 cut(s) 1384, 1638, 2943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.