Rroxscaffold_3G00247850

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000379
Physical Location & Seq
Reverse (-)
41210525 .. 41216265
5741 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00247850.1

Sequence Viewer

Length: 3342 bp
ATGGCCTCCTCTGCATCTCTTCCTTCATCAGTTGAATCATCATCAGCTCGTCCGTGGAGGTTTGATGTGTTCTTGAGTTTCAGGGGCCCTGACACTCGAAGGAGCATCACATCTGATCTATACCATCGACTGCAGAAGAGGGGAATTAAAACATTCATGGATGATCCAGATCTTCAAGTAGGGGATGTGATTTCTCCCGCTCTCCTAAAGGCAATTGAAGGATCAAGGTTTGCAATTGTTGTTCTGTCGGAAAAGTATGCTCATTCTACTTGGTGTTTGGAGGAACTTACAAAGATCTGTGAGTGTATGGAAGACGACAACCGAATTTGGCCACTTTTTTATGATGTGGAACCCTCTGATGTACGGTACCGGAAGAGCAGTTTCGGAGAAGCTTTCGATAAGCATAATAGCTCCGGGCGACACACATCACAGAATGTGCAGCGGTGGAATGATGCTTTAAACAAAGTAGCCAGTTTCTCTGGGTGGCATACAAAGAATTTTAAGACTGATAGAGAACTTGTGGTGCAAATTGTGGATTTTGTGTGCGGTAAAATACAACCGGAGATTACAATGTCTACTGGAGATTTTGAAGCGTTTGAAGCAACAAAAACAGCCATGGATAAGGTTATGAAGGCGCTTAAAGATGATGAGGTCACTGCCGTTGGTGTCTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAACCATGTTGCTGCACAATCCTGCAAAAGTGGGACTTTTCATCATAAGATTATGGCTGTTGTATCCCAAAGCCCCAACTTTGGAAGAATTCAAGGCACATTGGCAGATTTATTGGGCGTTAAATTGGAGGAGGAGACAGAAATTGGAAGAGCTGCTAGACTGAATAGGGAGATAATGAGAAGAGAAAAGATCCTTATAATCTTGGACGATGTTTGGAACAGAATAGAATTGTCATCCATAGGAATTCCTAGCTACGAGGAGCTTCAAAAGTGCAGTTCCAAAGTCATACTCACCACACGGAGAGGGAATGTCTGTCATGCAATGAAGTGCCAAGAAAAGGTCACCCTCAATATCCTCTCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAAGAAGGTCTTTTGAATCGATCACTTTTGAGGGTGTAGCGAGGAAAGTAGCTGGAGAATGCAGGGGTCTACCCATTGCATTGATAGCGGTTGCAAGGGCACTCGGAGATAAAGATCAGGTGGAATGGCAAAAAGCAGCTCGAAGATTAGAGAAGTCGCAATCTGCAACCGTTGAGGATGAGCAAGATGCCTTCCAATGTATAAAATTGAGCTATGATTACTTGAAAGATGAGGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGACGAGGACATCCGACTAGAAAAGTTATTCAAGTATGTAATCGGGACAGGATTGTTTCTACGAGATGCCGAAACAATCGAAGAAGCCAGAGGAACAGCAGATTCTGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGCGAGGAAAATGGATGTGTAAAGATGCATGATGTCATACGGGATACAGCCCTGAATATTGCAAAATCTAAAGATGGGCACGGGTTTCTGGTAAAAGCTGGCTGTGGTTTAAAGGATTGGCGGCCATGTGGATCATATCAAGGCTACTCTGCGATCTCACTGATGAGGAACGAAATTCGCAAGCTGCCCAAAGAAGAGTTGGTGTGTCCGAATCTCCAGATTTTGTTACTAGACCGCAACATTTATTTAACCAAGATCCCTGAAGAGTTTATCCAAAGTCTGAATGAATTAAGGCTCTTGGATCTTAGCGCCACTGGTATTTCCTCTCTACCCCAATCATTCGGTCTCCTTACCAATCTCCAAGCTTTGTATTTAGACTCTTGCTATAAGTTGATTGACATTTCTGTAGTCGGAAAACTGAAGAAACTTGAAATTCTTGGTATGATGGCATATCCTGGGGGGGAATTGTCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGAATGTCAGTGGTAGCCGTATGGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTGCTGTGCATTTGAGGACTGGGGGTGTGAAGCTGAGGGACAAGGAGAAGAAACCAATATTTTTGATGAGGTAGCTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCATATCTGATGCAGAATACATCCGTAAAAATGTTGAGGTCAAACCAGATTGGGATTACTTTTATATATGTATCGGCAGCGGATGGGGCTCCCGTGCATACAATATAGGAGATCATAATTTAAGATCCTTGTATTTTAGTGGAGCAACCATAAGTATCTTACCTGATTGGTTTATCAATGCAGTGACAAAGAAAACAGAGAAGCTAGAGTATTATTGCTGCGAAGGGATGAATGACATTCTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTACGTGGTAGTTGTCGTGATTCCTATGTGTACTTGAAAGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTGAAAACTTGGAAGAGTTGCGTCTGAAAGATCTTATCCACCTTGAGGAGTTGTGTGTTGGTGAGTTACCGCCTGGGTCTCTTTCCAATCTGAAGGTATTGCATATGTCTTGTTGTTTTATCTTGAAGAATGTATCAAAATTGGTACAGAGACTACCAAATTTGGAGAAACTGGTTTTAATTCGTATGCATAATTTAGAATGTGAGTTTGGATGCGAAGGCTTTGAGCCGAAACAATCAAAATTGAGAGAGATGGATTCGGTGGGTCCAAATTCAGCGAAAAGCATATGTAATGGTCCTGCCCCACGTGCAATGTTCCAGAGTCTTAGAAGTTTGGCTATTGGGAATTGTAAGTTGCTGCAAAGTCTGTTCGCATCTGATGTAGCCCGATGTCTTGTTCAATTGGAAGACCTTTTTGTAGAGGTGTGCCCTTTGTTGGAAAAAGTAATTGAAGCAGTGAACAACGAGAAGACGGTTCTACCAAAATTGAAGAACTTGGTTTTGAATAATCTTCCTAAGTTGTATGGTGCAAGTGGCACTGTTGATATTGAGTGTCCTTTATTGGAACACTTGATCGTGGTGGATTGCCCCCAGATTTCATTTTCAACCACTTCCTCTCTTTTGGAAGCCTTTGAAAGCTGGAACCAGTTTTCATGTTCAACCTCTGCTTCTGACTACTTTGGCAGCATGAACCCAGTCCAACTCAATGATCCAAAACTGGTCACATTTCTACGTAAGAAAATAACCAAGTTCAATTGGTGA

Protein Analysis

1113

Amino Acids

125.39

Weight (kDa)

6.04

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 21 - 183 3.1e-45 TIR domain
TIR_2 PF13676 23 - 118 9.6e-16 TIR domain
NB-ARC PF00931 203 - 366 2.5e-31 NB-ARC domain
LRR_8 PF13855 576 - 634 1.7e-08 Leucine rich repeat
LRR_14 PF23598 585 - 702 4.3e-15 Leucine-rich repeat region
LRR_14 PF23598 641 - 702 5e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 951 - 1058 1.6e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
Acc36I ACCTGC 1 cut(s) 2197
Acc65I GGTACC 1 cut(s) 366
AccB1I GGYRCC 1 cut(s) 366
AccBSI CCGCTC 1 cut(s) 200
AccI GTMKAC 3 cut(s) 575, 669, 1168
AciI CCGC 8 cut(s) 198, 442, 546, 1187, 1639, 1753, 2292, 2651
AclWI GGATC 8 cut(s) 158, 229, 889, 1657, 1768, 1827, 2331, 3284
AcoI YGGCCR 2 cut(s) 329, 1640
AcuI CTGAAG 3 cut(s) 1800, 1958, 2693
AcvI CACGTG 1 cut(s) 2888
AcyI GRCGYC 1 cut(s) 684
AfaI GTAC 7 cut(s) 363, 368, 1490, 2099, 2502, 2531, 2727
AfiI CCNNNNNNNGG 5 cut(s) 785, 1042, 2016, 2626, 3016
AjnI CCWGG 2 cut(s) 1972, 2653
AjuI GAANNNNNNNTTGG 4 cut(s) 1331, 1363, 2772, 2804
Alw26I GTCTC 4 cut(s) 833, 1868, 2664, 2725
AlwI GGATC 8 cut(s) 158, 229, 889, 1657, 1768, 1827, 2331, 3284
AlwNI CAGNNNCTG 1 cut(s) 1478
AoxI GGCC 4 cut(s) 3, 85, 329, 1640
ApaI GGGCCC 1 cut(s) 89
ApeKI GCWGC 9 cut(s) 439, 716, 857, 1235, 1702, 2289, 2430, 2938, 3264
Asp718I GGTACC 1 cut(s) 366
AspLEI GCGC 2 cut(s) 637, 1829
AspS9I GGNCC 6 cut(s) 85, 86, 1080, 2573, 2846, 2876
AsuC2I CCSGG 1 cut(s) 415
AsuHPI GGTGA 3 cut(s) 988, 1039, 2654
AvaII GGWCC 4 cut(s) 1080, 2573, 2846, 2876
BaeGI GKGCMC 4 cut(s) 89, 1201, 1599, 3011
BalI TGGCCA 1 cut(s) 331
BanI GGYRCC 1 cut(s) 366
BanII GRGCYC 2 cut(s) 89, 2303
BbrPI CACGTG 1 cut(s) 2888
BbsI GAAGAC 4 cut(s) 318, 1380, 2994, 3056
BbvCI CCTCAGC 1 cut(s) 2139
BbvI GCAGC 9 cut(s) 451, 703, 844, 1247, 1689, 2301, 2417, 2925, 3276
BccI CCATC 6 cut(s) 132, 1586, 1957, 2065, 2289, 2827
BceAI ACGGC 3 cut(s) 644, 688, 2025
BciT130I CCWGG 2 cut(s) 1974, 2655
BciVI GTATCC 2 cut(s) 778, 1555
BcnI CCSGG 1 cut(s) 415
BcoDI GTCTC 4 cut(s) 833, 1868, 2664, 2725
BfaI CTAG 6 cut(s) 861, 954, 1391, 1503, 1748, 2417
BfmI CTRYAG 2 cut(s) 131, 1923
BfoI RGCGCY 2 cut(s) 638, 1830
BfuAI ACCTGC 1 cut(s) 2197
BfuI GTATCC 2 cut(s) 778, 1555
BglII AGATCT 3 cut(s) 169, 294, 2611
Bme1390I CCNGG 3 cut(s) 415, 1974, 2655
Bme18I GGWCC 4 cut(s) 1080, 2573, 2846, 2876
BmgT120I GGNCC 6 cut(s) 85, 86, 1080, 2573, 2846, 2876
BmiI GGNNCC 7 cut(s) 86, 87, 351, 368, 2302, 2847, 3224
BmrFI CCNGG 3 cut(s) 415, 1974, 2655
BmrI ACTGGG 2 cut(s) 2134, 3269
BmuI ACTGGG 2 cut(s) 2134, 3269
BpiI GAAGAC 4 cut(s) 318, 1380, 2994, 3056
BpmI CTGGAG 3 cut(s) 600, 1173, 1718
Bpu10I CCTNAGC 1 cut(s) 2139
BpuEI CTTGAG 3 cut(s) 94, 2471, 2645
BpuMI CCSGG 1 cut(s) 415
Bsa29I ATCGAT 1 cut(s) 1118
BsaAI YACGTR 3 cut(s) 2504, 2888, 3314
BsaBI GATNNNNATC 2 cut(s) 168, 1212
BsaHI GRCGYC 1 cut(s) 684
BsaI GGTCTC 2 cut(s) 1868, 2664
BsaJI CCNNGG 6 cut(s) 53, 615, 699, 1973, 2467, 2654
BsaWI WCCGGW 2 cut(s) 369, 559
Bsc4I CCNNNNNNNGG 5 cut(s) 785, 1042, 2016, 2626, 3016
Bse1I ACTGG 9 cut(s) 471, 583, 1837, 2129, 2576, 2757, 3226, 3275, 3303
Bse3DI GCAATG 3 cut(s) 1032, 1173, 2898
Bse8I GATNNNNATC 2 cut(s) 168, 1212
BseBI CCWGG 2 cut(s) 1974, 2655
BseCI ATCGAT 1 cut(s) 1118
BseDI CCNNGG 6 cut(s) 53, 615, 699, 1973, 2467, 2654
BseJI GATNNNNATC 2 cut(s) 168, 1212
BseLI CCNNNNNNNGG 5 cut(s) 785, 1042, 2016, 2626, 3016
BseMI GCAATG 3 cut(s) 1032, 1173, 2898
BseMII CTCAG 2 cut(s) 1077, 2130
BseNI ACTGG 9 cut(s) 471, 583, 1837, 2129, 2576, 2757, 3226, 3275, 3303
BseRI GAGGAG 4 cut(s) 848, 851, 977, 2642
BseSI GKGCMC 4 cut(s) 89, 1201, 1599, 3011
BseXI GCAGC 9 cut(s) 451, 703, 844, 1247, 1689, 2301, 2417, 2925, 3276
BsgI GTGCAG 3 cut(s) 458, 702, 997
BshFI GGCC 4 cut(s) 5, 87, 331, 1642
BshNI GGYRCC 1 cut(s) 366
BshVI ATCGAT 1 cut(s) 1118
BsiSI CCGG 3 cut(s) 370, 414, 560
BslFI GGGAC 3 cut(s) 751, 1432, 2157
BslI CCNNNNNNNGG 5 cut(s) 785, 1042, 2016, 2626, 3016
BsmAI GTCTC 4 cut(s) 833, 1868, 2664, 2725
BsmFI GGGAC 3 cut(s) 751, 1432, 2157
BsmI GAATGC 1 cut(s) 1163
BsnI GGCC 4 cut(s) 5, 87, 331, 1642
Bso31I GGTCTC 2 cut(s) 1868, 2664
Bsp120I GGGCCC 1 cut(s) 85
Bsp1286I GDGCHC 5 cut(s) 89, 1201, 1599, 2303, 3011
Bsp1407I TGTACA 1 cut(s) 2097
Bsp19I CCATGG 3 cut(s) 615, 699, 2467
BspACI CCGC 8 cut(s) 198, 442, 546, 1187, 1639, 1753, 2292, 2651
BspANI GGCC 4 cut(s) 5, 87, 331, 1642
BspCNI CTCAG 2 cut(s) 1076, 2131
BspDI ATCGAT 1 cut(s) 1118
BspLI GGNNCC 7 cut(s) 86, 87, 351, 368, 2302, 2847, 3224
BspMAI CTGCAG 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 2197
BspPI GGATC 8 cut(s) 158, 229, 889, 1657, 1768, 1827, 2331, 3284
BspQI GCTCTTC 2 cut(s) 368, 847
BspT107I GGYRCC 1 cut(s) 366
BspTNI GGTCTC 2 cut(s) 1868, 2664
BsrBI CCGCTC 1 cut(s) 200
BsrDI GCAATG 3 cut(s) 1032, 1173, 2898
BsrGI TGTACA 1 cut(s) 2097
BsrI ACTGG 9 cut(s) 471, 583, 1837, 2129, 2576, 2757, 3226, 3275, 3303
BssECI CCNNGG 6 cut(s) 53, 615, 699, 1973, 2467, 2654
BssNI GRCGYC 1 cut(s) 684
BssT1I CCWWGG 3 cut(s) 615, 699, 2467
Bst2UI CCWGG 2 cut(s) 1974, 2655
Bst4CI ACNGT 5 cut(s) 366, 1270, 2500, 3055, 3121
Bst6I CTCTTC 8 cut(s) 24, 131, 368, 847, 880, 1707, 1776, 2589
BstACI GRCGYC 1 cut(s) 684
BstAUI TGTACA 1 cut(s) 2097
BstBAI YACGTR 3 cut(s) 2504, 2888, 3314
BstC8I GCNNGC 3 cut(s) 1508, 1618, 1700
BstDEI CTNAG 6 cut(s) 1063, 1823, 2015, 2139, 2906, 3096
BstDSI CCRYGG 4 cut(s) 53, 615, 699, 2467
BstEII GGTNACC 1 cut(s) 1045
BstH2I RGCGCY 2 cut(s) 638, 1830
BstHHI GCGC 2 cut(s) 637, 1829
BstMAI GTCTC 4 cut(s) 833, 1868, 2664, 2725
BstMWI GCNNNNNNNGC 6 cut(s) 11, 599, 681, 1184, 2298, 2888
BstNI CCWGG 2 cut(s) 1974, 2655
BstPI GGTNACC 1 cut(s) 1045
BstSCI CCNGG 3 cut(s) 413, 1972, 2653
BstSFI CTRYAG 2 cut(s) 131, 1923
BstSLI GKGCMC 4 cut(s) 89, 1201, 1599, 3011
BstSNI TACGTA 1 cut(s) 3314
BstV1I GCAGC 9 cut(s) 451, 703, 844, 1247, 1689, 2301, 2417, 2925, 3276
BstV2I GAAGAC 4 cut(s) 318, 1380, 2994, 3056
BstX2I RGATCY 7 cut(s) 169, 294, 894, 1773, 1819, 2336, 2611
BstYI RGATCY 7 cut(s) 169, 294, 894, 1773, 1819, 2336, 2611
Bsu15I ATCGAT 1 cut(s) 1118
BsuI GTATCC 2 cut(s) 778, 1555
BsuRI GGCC 4 cut(s) 5, 87, 331, 1642
BsuTUI ATCGAT 1 cut(s) 1118
BtgI CCRYGG 4 cut(s) 53, 615, 699, 2467
BtsI GCAGTG 3 cut(s) 654, 2400, 3042
BtsIMutI CAGTG 8 cut(s) 654, 1676, 1830, 2038, 2400, 2583, 3042, 3117
BveI ACCTGC 1 cut(s) 2197
Cac8I GCNNGC 3 cut(s) 1508, 1618, 1700
CaiI CAGNNNCTG 1 cut(s) 1478
CfoI GCGC 2 cut(s) 637, 1829
Cfr13I GGNCC 6 cut(s) 85, 86, 1080, 2573, 2846, 2876
ClaI ATCGAT 1 cut(s) 1118
CseI GACGC 2 cut(s) 673, 2591
Csp6I GTAC 7 cut(s) 362, 367, 1489, 2098, 2501, 2530, 2726
CviQI GTAC 7 cut(s) 362, 367, 1489, 2098, 2501, 2530, 2726
DdeI CTNAG 6 cut(s) 1063, 1823, 2015, 2139, 2906, 3096
DraI TTTAAA 2 cut(s) 459, 1629
EaeI YGGCCR 2 cut(s) 329, 1640
Eam1104I CTCTTC 8 cut(s) 24, 131, 368, 847, 880, 1707, 1776, 2589
EarI CTCTTC 8 cut(s) 24, 131, 368, 847, 880, 1707, 1776, 2589
Eco105I TACGTA 1 cut(s) 3314
Eco130I CCWWGG 3 cut(s) 615, 699, 2467
Eco24I GRGCYC 2 cut(s) 89, 2303
Eco31I GGTCTC 2 cut(s) 1868, 2664
Eco32I GATATC 1 cut(s) 2070
Eco47I GGWCC 4 cut(s) 1080, 2573, 2846, 2876
Eco57I CTGAAG 3 cut(s) 1800, 1958, 2693
Eco72I CACGTG 1 cut(s) 2888
Eco91I GGTNACC 1 cut(s) 1045
EcoO109I RGGNCCY 2 cut(s) 85, 86
EcoO65I GGTNACC 1 cut(s) 1045
EcoRI GAATTC 2 cut(s) 792, 948
EcoRII CCWGG 2 cut(s) 1972, 2653
EcoRV GATATC 1 cut(s) 2070
EcoT14I CCWWGG 3 cut(s) 615, 699, 2467
EcoT22I ATGCAT 2 cut(s) 1548, 2772
EcoT38I GRGCYC 2 cut(s) 89, 2303
ErhI CCWWGG 3 cut(s) 615, 699, 2467
FalI AAGNNNNNCTT 6 cut(s) 724, 756, 1093, 1125, 1331, 1363
FaqI GGGAC 3 cut(s) 751, 1432, 2157
FauI CCCGC 1 cut(s) 205
FauNDI CATATG 2 cut(s) 2685, 2867
FblI GTMKAC 3 cut(s) 575, 669, 1168
FriOI GRGCYC 2 cut(s) 89, 2303
FspBI CTAG 6 cut(s) 861, 954, 1391, 1503, 1748, 2417
GlaI GCGC 2 cut(s) 636, 1828
GsuI CTGGAG 3 cut(s) 600, 1173, 1718
HaeII RGCGCY 2 cut(s) 638, 1830
HaeIII GGCC 4 cut(s) 5, 87, 331, 1642
HapII CCGG 3 cut(s) 370, 414, 560
HgaI GACGC 2 cut(s) 673, 2591
HhaI GCGC 2 cut(s) 637, 1829
Hin1I GRCGYC 1 cut(s) 684
Hin6I GCGC 2 cut(s) 635, 1827
HinP1I GCGC 2 cut(s) 635, 1827
HindIII AAGCTT 2 cut(s) 390, 1881
HpaII CCGG 3 cut(s) 370, 414, 560
HphI GGTGA 3 cut(s) 988, 1039, 2654
Hpy166II GTNNAC 6 cut(s) 576, 670, 706, 1169, 2530, 3040
Hpy188III TCNNGA 8 cut(s) 73, 167, 1417, 1735, 1989, 2516, 2704, 2899
Hpy8I GTNNAC 6 cut(s) 576, 670, 706, 1169, 2530, 3040
Hpy99I CGWCG 1 cut(s) 689
HpyAV CCTTC 9 cut(s) 33, 93, 212, 625, 1098, 1300, 2429, 2668, 2792
HpyCH4III ACNGT 5 cut(s) 366, 1270, 2500, 3055, 3121
HpyCH4IV ACGT 3 cut(s) 2503, 2887, 3313
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 599, 681, 1184, 2298, 2888
HpyF3I CTNAG 6 cut(s) 1063, 1823, 2015, 2139, 2906, 3096
HpySE526I ACGT 3 cut(s) 2503, 2887, 3313
Hsp92I GRCGYC 1 cut(s) 684
HspAI GCGC 2 cut(s) 635, 1827
KpnI GGTACC 1 cut(s) 370
LguI GCTCTTC 2 cut(s) 368, 847
LmnI GCTCC 5 cut(s) 102, 416, 964, 2306, 2354
Lsp1109I GCAGC 9 cut(s) 451, 703, 844, 1247, 1689, 2301, 2417, 2925, 3276
MaeI CTAG 6 cut(s) 861, 954, 1391, 1503, 1748, 2417
MaeII ACGT 3 cut(s) 2503, 2887, 3313
MaeIII GTNAC 8 cut(s) 652, 1045, 1743, 2047, 2395, 2475, 2646, 3301
MbiI CCGCTC 1 cut(s) 200
MfeI CAATTG 4 cut(s) 213, 234, 2981, 3334
MflI RGATCY 7 cut(s) 169, 294, 894, 1773, 1819, 2336, 2611
MhlI GDGCHC 5 cut(s) 89, 1201, 1599, 2303, 3011
MlsI TGGCCA 1 cut(s) 331
MluNI TGGCCA 1 cut(s) 331
MlyI GAGTC 2 cut(s) 1889, 2911
MmeI TCCRAC 5 cut(s) 228, 1411, 1909, 2997, 3304
Mox20I TGGCCA 1 cut(s) 331
Mph1103I ATGCAT 2 cut(s) 1548, 2772
MscI TGGCCA 1 cut(s) 331
MslI CAYNNNNRTG 1 cut(s) 2453
Msp20I TGGCCA 1 cut(s) 331
MspA1I CMGCKG 2 cut(s) 442, 2292
MspI CCGG 3 cut(s) 370, 414, 560
MspR9I CCNGG 3 cut(s) 415, 1974, 2655
MunI CAATTG 4 cut(s) 213, 234, 2981, 3334
Mva1269I GAATGC 1 cut(s) 1163
MvaI CCWGG 2 cut(s) 1974, 2655
MwoI GCNNNNNNNGC 6 cut(s) 11, 599, 681, 1184, 2298, 2888
NciI CCSGG 1 cut(s) 415
NcoI CCATGG 3 cut(s) 615, 699, 2467
NdeI CATATG 2 cut(s) 2685, 2867
NlaIV GGNNCC 7 cut(s) 86, 87, 351, 368, 2302, 2847, 3224
NmuCI GTSAC 5 cut(s) 652, 1045, 2047, 2395, 3301
NsiI ATGCAT 2 cut(s) 1548, 2772
PciSI GCTCTTC 2 cut(s) 368, 847
PctI GAATGC 1 cut(s) 1163
PfeI GAWTC 9 cut(s) 35, 1073, 1115, 1334, 1475, 1499, 1729, 2519, 2837
PleI GAGTC 2 cut(s) 1889, 2910
PmaCI CACGTG 1 cut(s) 2888
PmlI CACGTG 1 cut(s) 2888
PpsI GAGTC 2 cut(s) 1889, 2910
Ppu21I YACGTR 3 cut(s) 2504, 2888, 3314
PsiI TTATAA 1 cut(s) 902
Psp6I CCWGG 2 cut(s) 1972, 2653
PspCI CACGTG 1 cut(s) 2888
PspEI GGTNACC 1 cut(s) 1045
PspGI CCWGG 2 cut(s) 1972, 2653
PspN4I GGNNCC 7 cut(s) 86, 87, 351, 368, 2302, 2847, 3224
PspOMI GGGCCC 1 cut(s) 85
PspPI GGNCC 6 cut(s) 85, 86, 1080, 2573, 2846, 2876
PstI CTGCAG 1 cut(s) 135
PstNI CAGNNNCTG 1 cut(s) 1478
PsuI RGATCY 7 cut(s) 169, 294, 894, 1773, 1819, 2336, 2611
RsaI GTAC 7 cut(s) 363, 368, 1490, 2099, 2502, 2531, 2727
RsaNI GTAC 7 cut(s) 362, 367, 1489, 2098, 2501, 2530, 2726
RseI CAYNNNNRTG 1 cut(s) 2453
SapI GCTCTTC 2 cut(s) 368, 847
Sau96I GGNCC 6 cut(s) 85, 86, 1080, 2573, 2846, 2876
SchI GAGTC 2 cut(s) 1889, 2911
ScrFI CCNGG 3 cut(s) 415, 1974, 2655
SduI GDGCHC 5 cut(s) 89, 1201, 1599, 2303, 3011
SfcI CTRYAG 2 cut(s) 131, 1923
SinI GGWCC 4 cut(s) 1080, 2573, 2846, 2876
SmiMI CAYNNNNRTG 1 cut(s) 2453
SmlI CTYRAG 3 cut(s) 73, 2486, 2624
SmoI CTYRAG 3 cut(s) 73, 2486, 2624
SnaBI TACGTA 1 cut(s) 3314
SsiI CCGC 8 cut(s) 198, 442, 546, 1187, 1639, 1753, 2292, 2651
SspI AATATT 3 cut(s) 1576, 2164, 2202
SspMI CTAG 6 cut(s) 861, 954, 1391, 1503, 1748, 2417
StyD4I CCNGG 3 cut(s) 413, 1972, 2653
StyI CCWWGG 3 cut(s) 615, 699, 2467
TaaI ACNGT 5 cut(s) 366, 1270, 2500, 3055, 3121
TaiI ACGT 3 cut(s) 2506, 2890, 3316
TaqI TCGA 7 cut(s) 97, 127, 396, 1118, 1240, 1452, 1988
TaqII GACCGA 1 cut(s) 1850
TatI WGTACW 2 cut(s) 2097, 2529
TauI GCSGC 1 cut(s) 1642
TfiI GAWTC 9 cut(s) 35, 1073, 1115, 1334, 1475, 1499, 1729, 2519, 2837
TscAI CASTG 8 cut(s) 661, 1683, 1837, 2038, 2400, 2583, 3042, 3124
TseFI GTSAC 5 cut(s) 652, 1045, 2047, 2395, 3301
TseI GCWGC 9 cut(s) 439, 716, 857, 1235, 1702, 2289, 2430, 2938, 3264
Tsp45I GTSAC 5 cut(s) 652, 1045, 2047, 2395, 3301
TspGWI ACGGA 3 cut(s) 42, 1018, 2225
TspRI CASTG 8 cut(s) 661, 1683, 1837, 2038, 2400, 2583, 3042, 3124
VpaK11BI GGWCC 4 cut(s) 1080, 2573, 2846, 2876
XcmI CCANNNNNNNNNTGG 1 cut(s) 1714
XmiI GTMKAC 3 cut(s) 575, 669, 1168
XspI CTAG 6 cut(s) 861, 954, 1391, 1503, 1748, 2417
Zsp2I ATGCAT 2 cut(s) 1548, 2772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.