RchiOBHm_Chr5g0078321

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
84226188 .. 84227692
1505 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35285

Sequence Viewer

Length: 1440 bp
ATGTATGAGATTATGAAGGCGTTTACAGATGATGACGTCACTGCAATTGGTGTCTACGGCATGGGAGGCGTTGGAAAGACAACCATGGTAAAACATGTCGCTGCACATGCTAGAAAAAGTGGGATTTTTGATCATGTGATTATGGGTGTCGTATCCCAAAACCCTGACTTGAAAAGAATTCAAGACACATTGGCAGAGTTGCTGGGCTTCGAATTACATGAGGGGACAGAAATTGGAAGAGCAGCTAGATTGAGCAAGGAGATAAAGAGAAGAAATAAGATCCTTATAATCCTGGACGACATTTGGGAGAGAATGGAATTGTCAGACATAGGAATACCTAGCTACAAGGAACTTCGAAAGTGCAAATCCAAAGTGCTACTCACCACAAGGATAAGAAATGTGTGTCATGCGATGAAGTGCCAAGAAAAGATCAACCTCACTACCCTATCAGAAGAAGATTCTTGGACCTTGTTTGTGAGAAATGCAGGAAGGTCTTTTGAATCCACCAATTTCGAGGATGTTGCGAGGAGGGTAGCTGGAGAGTGTTGCGGTTTACCGATTGCATTGATAGCAGTTGCAAGGGCACTCGGAGGTAAAGACTTGGTGGAATGGAAGAGAGCAGCTCAAAGATTAGAGAAGTCTCATGCTGCCAACCCTGAGGATTACGGAGAGGCATCCAAATGTATAAAATTAAGCTATGATTACTTGAAAGAGGAGGACTATAAGTCATACTTCTTGCTCTGTTGCTTATTCCCAGAAGACTTTGAAATCGAAATAGAAGACTTGTTCAAGTATGCGATCGGGAAAGGGTTGTTTCGAGATGCCGACACAATCGAAGAAGCCAGAGACGAAGCTGATTCCGTGGCCACACACCTTAAACATTGTTGCTTGCTTTTGGATGGTGAAAACAATAAATTTGTAAGGATGCATGATGTTGTCCGGGATACAGCCATTCAAATTGCGCAATCTGAAGATGGGCATGGGTTTTTCGTGAAAGCTGGCTGTGGTTTAAAGGATTGGCCTCCTCGATTACATGAAGGCTATTCGGCAATCTCACTAATGGCTAATTGTATTGCCAAGCTACCTGAAAAGTTGGTATGTCCAAAACTCCAAATTTTGTTACTCAACTCTAATTCCTCTATAGGAGAGATCCCAGAATCCTTTTTTCAAAGTCCGAATGAATTAACGGTCTTAGATCTTACGCGGACCCGTATTTCATTACTACCCCAATCATTCAGTCTGCTAACCAATCTCCAAGCTTTGTATTTAGATCGTTGTTTCAAATTTATTGACACTTCTAAACTGGGAGAACTTAAGAAGCTTGAGATTCTTAGTATGAGAAATTCATGTTTTAAGGGATTGTCGAGAGAAATAGGAGATTTGACCAATCTAAGGATGTTGGATATCACCAGTAAAAGGATTTTGGAACTGGGGGAGTAA

Protein Analysis

479

Amino Acids

54.06

Weight (kDa)

6.08

Isoelectric Point (pI)

40.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 6 - 162 2.6e-31 NB-ARC domain
LRR_8 PF13855 368 - 425 6.4e-10 Leucine rich repeat
LRR_14 PF23598 378 - 471 1.3e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 287
AatII GACGTC 1 cut(s) 39
Acc16I TGCGCA 1 cut(s) 963
AccI GTMKAC 1 cut(s) 54
AccII CGCG 1 cut(s) 1204
AciI CCGC 2 cut(s) 549, 1204
AclWI GGATC 2 cut(s) 274, 1144
AcoI YGGCCR 1 cut(s) 864
AcsI RAATTY 5 cut(s) 177, 914, 1113, 1283, 1342
AcuI CTGAAG 1 cut(s) 990
AcyI GRCGYC 1 cut(s) 36
AfiI CCNNNNNNNGG 2 cut(s) 1392, 1416
AflII CTTAAG 1 cut(s) 1313
AflIII ACRYGT 1 cut(s) 94
AgsI TTSAA 9 cut(s) 172, 182, 500, 709, 767, 790, 956, 1169, 1282
AhdI GACNNNNNGTC 1 cut(s) 724
AjnI CCWGG 1 cut(s) 291
Alw26I GTCTC 2 cut(s) 645, 840
AlwI GGATC 2 cut(s) 274, 1144
AoxI GGCC 2 cut(s) 864, 1019
ApeKI GCWGC 4 cut(s) 101, 242, 620, 647
ApoI RAATTY 5 cut(s) 177, 914, 1113, 1283, 1342
AspLEI GCGC 1 cut(s) 964
AspS9I GGNCC 2 cut(s) 465, 1206
AsuC2I CCSGG 1 cut(s) 941
AsuHPI GGTGA 3 cut(s) 373, 914, 1399
AsuII TTCGAA 2 cut(s) 210, 355
AvaII GGWCC 2 cut(s) 465, 1206
AxyI CCTNAGG 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 586
BalI TGGCCA 1 cut(s) 866
BbsI GAAGAC 2 cut(s) 765, 786
BbvI GCAGC 4 cut(s) 88, 254, 632, 634
BccI CCATC 2 cut(s) 893, 968
BceAI ACGGC 1 cut(s) 73
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 1 cut(s) 293
BciVI GTATCC 2 cut(s) 163, 937
BclI TGATCA 1 cut(s) 130
BcnI CCSGG 1 cut(s) 941
BcoDI GTCTC 2 cut(s) 645, 840
BfaI CTAG 3 cut(s) 111, 246, 339
BfmI CTRYAG 1 cut(s) 1140
BfrI CTTAAG 1 cut(s) 1313
BfuI GTATCC 2 cut(s) 163, 937
BglII AGATCT 1 cut(s) 1195
BisI GCNGC 4 cut(s) 102, 243, 621, 648
BlsI GCNGC 4 cut(s) 103, 244, 622, 649
Bme1390I CCNGG 2 cut(s) 293, 941
Bme18I GGWCC 2 cut(s) 465, 1206
BmeRI GACNNNNNGTC 1 cut(s) 724
BmgT120I GGNCC 2 cut(s) 465, 1206
BmiI GGNNCC 1 cut(s) 1208
BmrFI CCNGG 2 cut(s) 293, 941
BmrI ACTGGG 2 cut(s) 1313, 1439
BmsI GCATC 3 cut(s) 683, 811, 915
BmuI ACTGGG 2 cut(s) 1313, 1439
BpiI GAAGAC 2 cut(s) 765, 786
BplI GAGNNNNNCTC 2 cut(s) 607, 639
BpmI CTGGAG 1 cut(s) 558
Bpu14I TTCGAA 2 cut(s) 210, 355
BpuEI CTTGAG 1 cut(s) 1343
BpuMI CCSGG 1 cut(s) 941
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 84, 861
Bsc4I CCNNNNNNNGG 2 cut(s) 1392, 1416
Bse1I ACTGG 3 cut(s) 1308, 1410, 1434
Bse21I CCTNAGG 1 cut(s) 657
BseBI CCWGG 1 cut(s) 293
BseDI CCNNGG 2 cut(s) 84, 861
BseGI GGATG 5 cut(s) 523, 674, 904, 930, 1401
BseLI CCNNNNNNNGG 2 cut(s) 1392, 1416
BseMII CTCAG 1 cut(s) 648
BseNI ACTGG 3 cut(s) 1308, 1410, 1434
BseRI GAGGAG 3 cut(s) 541, 728, 1014
BseSI GKGCMC 1 cut(s) 586
BseXI GCAGC 4 cut(s) 88, 254, 632, 634
BseYI CCCAGC 1 cut(s) 202
BsgI GTGCAG 1 cut(s) 87
Bsh1236I CGCG 1 cut(s) 1204
Bsh1285I CGRYCG 1 cut(s) 801
BshFI GGCC 2 cut(s) 866, 1021
BsiEI CGRYCG 1 cut(s) 801
BsiSI CCGG 1 cut(s) 940
BslFI GGGAC 1 cut(s) 238
BslI CCNNNNNNNGG 2 cut(s) 1392, 1416
BsmAI GTCTC 2 cut(s) 645, 840
BsmBI CGTCTC 1 cut(s) 840
BsmFI GGGAC 1 cut(s) 238
BsnI GGCC 2 cut(s) 866, 1021
Bsp119I TTCGAA 2 cut(s) 210, 355
Bsp1286I GDGCHC 1 cut(s) 586
Bsp143I GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 2 cut(s) 549, 1204
BspANI GGCC 2 cut(s) 866, 1021
BspCNI CTCAG 1 cut(s) 649
BspFNI CGCG 1 cut(s) 1204
BspLI GGNNCC 1 cut(s) 1208
BspPI GGATC 2 cut(s) 274, 1144
BspQI GCTCTTC 1 cut(s) 232
BspT104I TTCGAA 2 cut(s) 210, 355
BspTI CTTAAG 1 cut(s) 1313
BsrI ACTGG 3 cut(s) 1308, 1410, 1434
BssECI CCNNGG 2 cut(s) 84, 861
BssMI GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 84
Bst2UI CCWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 1189
Bst6I CTCTTC 2 cut(s) 232, 608
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 1313
BstBI TTCGAA 2 cut(s) 210, 355
BstC8I GCNNGC 2 cut(s) 890, 1000
BstDEI CTNAG 4 cut(s) 657, 1192, 1331, 1391
BstDSI CCRYGG 2 cut(s) 84, 861
BstF5I GGATG 5 cut(s) 523, 674, 904, 930, 1401
BstFNI CGCG 1 cut(s) 1204
BstHHI GCGC 1 cut(s) 964
BstKTI GATC 7 cut(s) 133, 282, 432, 801, 1152, 1198, 1273
BstMAI GTCTC 2 cut(s) 645, 840
BstMBI GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
BstMCI CGRYCG 1 cut(s) 801
BstMWI GCNNNNNNNGC 3 cut(s) 66, 107, 569
BstNI CCWGG 1 cut(s) 293
BstNSI RCATGY 2 cut(s) 98, 110
BstSCI CCNGG 2 cut(s) 291, 939
BstSFI CTRYAG 1 cut(s) 1140
BstSLI GKGCMC 1 cut(s) 586
BstUI CGCG 1 cut(s) 1204
BstV1I GCAGC 4 cut(s) 88, 254, 632, 634
BstV2I GAAGAC 2 cut(s) 765, 786
BstX2I RGATCY 3 cut(s) 279, 1149, 1195
BstYI RGATCY 3 cut(s) 279, 1149, 1195
Bsu36I CCTNAGG 1 cut(s) 657
BsuI GTATCC 2 cut(s) 163, 937
BsuRI GGCC 2 cut(s) 866, 1021
BtgI CCRYGG 2 cut(s) 84, 861
BtgZI GCGATG 1 cut(s) 425
BtsCI GGATG 5 cut(s) 523, 674, 904, 930, 1401
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 890, 1000
CfoI GCGC 1 cut(s) 964
Cfr13I GGNCC 2 cut(s) 465, 1206
DdeI CTNAG 4 cut(s) 657, 1192, 1331, 1391
DpnI GATC 7 cut(s) 132, 281, 431, 800, 1151, 1197, 1272
DpnII GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
DraI TTTAAA 1 cut(s) 1011
DriI GACNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 864
Eam1104I CTCTTC 2 cut(s) 232, 608
Eam1105I GACNNNNNGTC 1 cut(s) 724
EarI CTCTTC 2 cut(s) 232, 608
Eco130I CCWWGG 1 cut(s) 84
Eco32I GATATC 1 cut(s) 1405
Eco47I GGWCC 2 cut(s) 465, 1206
Eco57I CTGAAG 1 cut(s) 990
Eco81I CCTNAGG 1 cut(s) 657
EcoRI GAATTC 1 cut(s) 177
EcoRII CCWGG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 1405
EcoT14I CCWWGG 1 cut(s) 84
EcoT22I ATGCAT 1 cut(s) 930
ErhI CCWWGG 1 cut(s) 84
Esp3I CGTCTC 1 cut(s) 840
FalI AAGNNNNNCTT 2 cut(s) 716, 748
FaqI GGGAC 1 cut(s) 238
FbaI TGATCA 1 cut(s) 130
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 4 cut(s) 102, 243, 621, 648
FokI GGATG 5 cut(s) 530, 661, 911, 937, 1408
Fsp4HI GCNGC 4 cut(s) 102, 243, 621, 648
FspBI CTAG 3 cut(s) 111, 246, 339
FspI TGCGCA 1 cut(s) 963
GlaI GCGC 1 cut(s) 963
GluI GCNGC 4 cut(s) 102, 243, 621, 648
GsaI CCCAGC 1 cut(s) 206
GsuI CTGGAG 1 cut(s) 558
HaeIII GGCC 2 cut(s) 866, 1021
HapII CCGG 1 cut(s) 940
HhaI GCGC 1 cut(s) 964
Hin1I GRCGYC 1 cut(s) 36
Hin6I GCGC 1 cut(s) 962
HinP1I GCGC 1 cut(s) 962
HindIII AAGCTT 2 cut(s) 1257, 1319
HinfI GANTC 5 cut(s) 458, 500, 857, 1157, 1327
HpaII CCGG 1 cut(s) 940
HphI GGTGA 3 cut(s) 373, 914, 1399
Hpy166II GTNNAC 3 cut(s) 24, 55, 554
Hpy188I TCNGA 5 cut(s) 325, 451, 590, 970, 1176
Hpy188III TCNNGA 5 cut(s) 182, 802, 818, 991, 1365
Hpy8I GTNNAC 3 cut(s) 24, 55, 554
HpyAV CCTTC 3 cut(s) 10, 483, 1031
HpyCH4III ACNGT 1 cut(s) 1189
HpyCH4IV ACGT 1 cut(s) 36
HpyCH4V TGCA 7 cut(s) 44, 104, 363, 485, 563, 578, 928
HpyF10VI GCNNNNNNNGC 3 cut(s) 66, 107, 569
HpyF3I CTNAG 4 cut(s) 657, 1192, 1331, 1391
HpySE526I ACGT 1 cut(s) 36
Hsp92I GRCGYC 1 cut(s) 36
HspAI GCGC 1 cut(s) 962
Ksp22I TGATCA 1 cut(s) 130
Kzo9I GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
LguI GCTCTTC 1 cut(s) 232
Lsp1109I GCAGC 4 cut(s) 88, 254, 632, 634
LweI GCATC 3 cut(s) 683, 811, 915
MaeI CTAG 3 cut(s) 111, 246, 339
MaeII ACGT 1 cut(s) 36
MaeIII GTNAC 2 cut(s) 37, 1119
MalI GATC 7 cut(s) 132, 281, 431, 800, 1151, 1197, 1272
MboI GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
MboII GAAGA 9 cut(s) 249, 282, 464, 467, 625, 770, 791, 848, 983
MfeI CAATTG 1 cut(s) 45
MflI RGATCY 3 cut(s) 279, 1149, 1195
MhlI GDGCHC 1 cut(s) 586
MlsI TGGCCA 1 cut(s) 866
MluNI TGGCCA 1 cut(s) 866
MmeI TCCRAC 2 cut(s) 52, 1380
Mox20I TGGCCA 1 cut(s) 866
Mph1103I ATGCAT 1 cut(s) 930
MscI TGGCCA 1 cut(s) 866
MseI TTAA 6 cut(s) 692, 876, 1010, 1184, 1314, 1353
MslI CAYNNNNRTG 1 cut(s) 679
Msp20I TGGCCA 1 cut(s) 866
MspCI CTTAAG 1 cut(s) 1313
MspI CCGG 1 cut(s) 940
MspR9I CCNGG 2 cut(s) 293, 941
MunI CAATTG 1 cut(s) 45
MvaI CCWGG 1 cut(s) 293
MvnI CGCG 1 cut(s) 1204
MwoI GCNNNNNNNGC 3 cut(s) 66, 107, 569
NciI CCSGG 1 cut(s) 941
NcoI CCATGG 1 cut(s) 84
NdeII GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
NlaIV GGNNCC 1 cut(s) 1208
NmuCI GTSAC 1 cut(s) 37
NsbI TGCGCA 1 cut(s) 963
NsiI ATGCAT 1 cut(s) 930
NspI RCATGY 2 cut(s) 98, 110
NspV TTCGAA 2 cut(s) 210, 355
PciI ACATGT 1 cut(s) 94
PciSI GCTCTTC 1 cut(s) 232
PfeI GAWTC 5 cut(s) 458, 500, 857, 1157, 1327
PfoI TCCNGGA 2 cut(s) 291, 939
PkrI GCNGC 4 cut(s) 103, 244, 622, 649
Ple19I CGATCG 1 cut(s) 801
PscI ACATGT 1 cut(s) 94
PsiI TTATAA 1 cut(s) 287
Psp6I CCWGG 1 cut(s) 291
PspFI CCCAGC 1 cut(s) 202
PspGI CCWGG 1 cut(s) 291
PspN4I GGNNCC 1 cut(s) 1208
PspPI GGNCC 2 cut(s) 465, 1206
PsuI RGATCY 3 cut(s) 279, 1149, 1195
PvuI CGATCG 1 cut(s) 801
RseI CAYNNNNRTG 1 cut(s) 679
SapI GCTCTTC 1 cut(s) 232
SaqAI TTAA 6 cut(s) 692, 876, 1010, 1184, 1314, 1353
SatI GCNGC 4 cut(s) 102, 243, 621, 648
Sau3AI GATC 7 cut(s) 130, 279, 429, 798, 1149, 1195, 1270
Sau96I GGNCC 2 cut(s) 465, 1206
ScrFI CCNGG 2 cut(s) 293, 941
SduI GDGCHC 1 cut(s) 586
SfaNI GCATC 3 cut(s) 683, 811, 915
SfcI CTRYAG 1 cut(s) 1140
SfuI TTCGAA 2 cut(s) 210, 355
SinI GGWCC 2 cut(s) 465, 1206
SmiMI CAYNNNNRTG 1 cut(s) 679
SmlI CTYRAG 2 cut(s) 1313, 1322
SmoI CTYRAG 2 cut(s) 1313, 1322
SsiI CCGC 2 cut(s) 549, 1204
SspMI CTAG 3 cut(s) 111, 246, 339
StyD4I CCNGG 2 cut(s) 291, 939
StyI CCWWGG 1 cut(s) 84
TaaI ACNGT 1 cut(s) 1189
TaiI ACGT 1 cut(s) 39
TaqI TCGA 8 cut(s) 210, 355, 513, 771, 817, 834, 1027, 1364
TfiI GAWTC 5 cut(s) 458, 500, 857, 1157, 1327
Tru1I TTAA 6 cut(s) 692, 876, 1010, 1184, 1314, 1353
Tru9I TTAA 6 cut(s) 692, 876, 1010, 1184, 1314, 1353
TscAI CASTG 1 cut(s) 46
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 4 cut(s) 101, 242, 620, 647
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 6 cut(s) 29, 428, 1050, 1194, 1206, 1335
TspGWI ACGGA 2 cut(s) 681, 850
TspRI CASTG 1 cut(s) 46
Vha464I CTTAAG 1 cut(s) 1313
VpaK11BI GGWCC 2 cut(s) 465, 1206
XapI RAATTY 5 cut(s) 177, 914, 1113, 1283, 1342
XceI RCATGY 2 cut(s) 98, 110
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 3 cut(s) 111, 246, 339
ZraI GACGTC 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.