Rw0G005160

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00204
OG0000379
Physical Location & Seq
Forward (+)
42322 .. 46287
3966 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005160.1

Sequence Viewer

Length: 3465 bp
ATGGTGTCCGACGCAAGCAAGAAGAAGGTGGCTCAGAAGAAGGCCGCCGCGCGGGTTGCTTCTTCTTCTTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGGATTACAGTCGACATACACGATCGACTACAGAGGAGGGGAATTAAAGCATTCATGGATGATCCAGATCTCCAAGTAGGGAATGTTATTTCTCCAACTCTCTTAAGGGCAATTAGAGAATCAAGGTTTGCAATCGTTGTTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACAACAGGATTTTGCCACTTTTTTATCATGTTAATCCTACTGATGTTCGATATCAGAAGAGGAGTTTCGAAGATGCTTTCTCTAAGCATGAAACCTCTGGGCGACATGAATCAGAGAAGGTGAAGCAATGGAAAGCTGCTTTAAACAAAGTGGCCAATATCTCTGGGTGGAATACAGATGATCATAAGACTCACCAAGAACTGATCGACTCCATCGTGGAATCTGTCTACCGTAAAGTCTTACCTGCTGCAGTTGTGCTCATGGGAGAGTTCGAAGTGTTTCAAGCAACGAGACAAGCCATGGATGAGGTTACGGAGGCCCTAAAAGACGATGAAGTCAATGCCGTTGGTGTGTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGGTGCACAAGCCTGCAAAAATGGGATTTTTGATCGTGTGATTATCGCTGTCGTTTCACAAAATCCTAATTTCGAAAAACTTCAAGACGCATTGGCAGAGCAGTTGGGCTTCAAATTGTCGGAGGAGACAGAAATTGGAAGAATTGCTAGATTGAAAAAGGAGATAATGAGCAAAGAAAAACTACTTATAATCTTGGACGATGTTTGGGGGAGAATAGAGTTGTCAAAGATTGGGATTCCCAGCTACAAGGAACTTCAAAAGTGCAAGTCCAAAGTCCTACTCACCACAAGGAGATGGAATGTTTGTCATGCCATGGAGAGCGATGCAAAAATTCAACTCAGTATCTTATCGGAAGAAGATGCTTGGAACTTGTTTTTGACCAAGGCAAGAAGGTCTTTTGAATCGACCTCTTTTGAGGGTGTAGCGAGGAAGGTAGCTGGTGAATGCGGGGGTCTGCCCATTGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGATCTGAAGGAATGGGAAAAGGCAGCTCAACAATTAAAGAAGTCGCAATTTGCCAACCCTGATCATGACGAAGATGCTTTCAGATGTATAAAATTAAGCTATGATTACTTGAAAGATGAGGACTACAAGTCATGCTTCTTGCTGTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCCGGTATGCAATTGGGAAAGGATTGTTTCGTGATACCGACACAATATATGAAGCCAGAGGAATCGCACATACAGTGGTCAAGCACCTGAAAGATTCTAGCTTGCTTTTGGAGAGTACATCCGATGGACTCTTTGGTGACTTTGGTACACTTGGAGAGCTGTGTTATGTCGATGGAGTCCTTGGTGAACGTGTAAAGATGCATGATGTCGTCCGGGATACAGCCATGAGAATTGCAAAATGTGAAGATGGGTTTTTGGTGAAAGCTGGATGGGGTTTAGATCATTGGTCATGTCGATCACATGAAGGCTACTCTGCAATCTCACTAGTGGAGAATAACATTCGCCGTCTACCCGAAGAGTTGGTAAGTCCAAAACTCCAGATTTTATTACTACAAAAGAATACTGATTTAAATGAGATCCCAGAGACCTTTTTCCCGAGTCCCAATGAATTAATGCTCTTGGATCTTAGCAAGACTAGTATTTCCTCTCTACCCCAATCTTTCAGTCTCCTTACCAACCTTCAAGTTTTGTACTTAGATTTTTGCGCCAAATTGACCGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGCATGAGGAACTATGGTTGGGGGAGATTGTTGCCGAGAGAAATAGGAAATTTGACCAATCTAAGGATTTTGGAAGTCAGTTGCACTTACGGTCTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGCACATGCTGGAGTGTAGACATATGGACTGGGGGTGTGAAATTGAGGGACAAGGAGGAGAAACCAATATTGGGTTTGATGACTTAGCTGGTTTATCATATTTGAAAGTTTTGCACGTTTACATATCTGATGCAAAATACATCCCTAAAAATGTTGAGGCCGAACCGAATTGGGTTTACTTTAACATATATATTGGCCACAGCAAGGAATTTCAATACGATGGAGAAGAACATAATTCAAAATCCTTGCATCTTATAGGACCAACCATAAGTACCTTGCCTGATTGGTTTATCAACGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATGATAGCTGCATATGGATGAGTGACATTCTTGTGAAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACAGTATCGTGTGATCTTGGTTATCTTGATGATGTTGGTGATCTTGTTGATCATTCCGATGAGTACTTGAAAGAGTTGATGAACACAACAAGACGAGTTCATCAAGGACCAGTTTTTGAGGAGTTGGAAGAGTTGCATCTGATAGAGATGGAACACCTGGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTTTCTCCAATCTCAAGGTATTGCATATGAGTTCATGTCATATGTTGAAGAATGTATCAAAATTGGTAAAGAGACTACCAAATCTGGAGAAACTAGTTTTAAACTATTTGCGCGATTTGGAATATGTGTTTGGATGCGAAGGGTTCGAGCCGAAAGAATCCAAACTGAGAGAGATGCATTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCCAGAGTCTTAAAAGTTTGATCTTTTCCGACTGTTGGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTGAGTGTCTTGTTCAATTGGAAGACCTTCTTGTAAAGCGGTGCTCTTTGTTGGAAAGAGTAATTGAAGCAGTGAACAATGAGAAGACGTCTCTACGAAAATTGAAGAACTTGGTTTTGATGAATCTTCCTAAGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTTATTGGAACGCTTGATTGTGGTGGATTGCCCCCAGATTTCATTTTCAACCTCTTTCTCTCTCTTCCATGACTTCGAAAGCAGGAACCAGTTTTCATTTTCAAACTCTGCTTCTGACTACTTTGGCAGCACGAACCCAGTCCAACTCAATGATCCACAACTGTACAGTTTGCTGAGTATGAGATGCGACAGAACATCTGGTGTCATATTGTAG

Protein Analysis

1154

Amino Acids

130.77

Weight (kDa)

5.71

Isoelectric Point (pI)

43.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 33 - 197 1e-45 TIR domain
TIR_2 PF13676 36 - 142 3.8e-15 TIR domain
NB-ARC PF00931 216 - 379 2.7e-32 NB-ARC domain
LRR_8 PF13855 603 - 661 1.4e-08 Leucine rich repeat
LRR_14 PF23598 612 - 736 2e-12 Leucine-rich repeat region
LRR_RPS2 PF23247 902 - 1041 2e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 982 - 1097 3.4e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 938
AasI GACNNNNNNGTC 1 cut(s) 686
AatII GACGTC 1 cut(s) 3170
Acc36I ACCTGC 1 cut(s) 604
AccI GTMKAC 4 cut(s) 153, 579, 1786, 2197
AccII CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
AciI CCGC 6 cut(s) 45, 48, 52, 1197, 2477, 3118
AclWI GGATC 4 cut(s) 197, 1849, 1908, 3398
AcoI YGGCCR 2 cut(s) 504, 2374
AcsI RAATTY 5 cut(s) 1080, 2031, 2086, 2387, 2965
AcuI CTGAAG 2 cut(s) 1274, 2039
AcyI GRCGYC 2 cut(s) 720, 3167
AfaI GTAC 7 cut(s) 707, 1555, 1585, 1970, 2452, 2645, 3416
AfiI CCNNNNNNNGG 5 cut(s) 51, 2100, 2250, 2383, 3045
AflII CTTAAG 1 cut(s) 244
AflIII ACRYGT 3 cut(s) 745, 1627, 3001
AhdI GACNNNNNGTC 1 cut(s) 1375
AhlI ACTAGT 3 cut(s) 1762, 1913, 2866
AjnI CCWGG 2 cut(s) 2736, 2767
AjuI GAANNNNNNNTTGG 4 cut(s) 2232, 2264, 2886, 2918
Alw21I GWGCWC 3 cut(s) 612, 757, 3125
Alw26I GTCTC 7 cut(s) 637, 869, 1856, 1949, 2135, 2839, 3174
Alw44I GTGCAC 1 cut(s) 753
AlwI GGATC 4 cut(s) 197, 1849, 1908, 3398
Ama87I CYCGRG 1 cut(s) 1873
AoxI GGCC 5 cut(s) 42, 504, 669, 2337, 2374
ApaLI GTGCAC 1 cut(s) 753
ApeKI GCWGC 7 cut(s) 488, 599, 1271, 2514, 3049, 3052, 3378
ApoI RAATTY 5 cut(s) 1080, 2031, 2086, 2387, 2965
AseI ATTAAT 1 cut(s) 1889
Asp700I GAANNNNTTC 3 cut(s) 630, 828, 3105
AspLEI GCGC 3 cut(s) 52, 1985, 2886
AspS9I GGNCC 6 cut(s) 125, 670, 2438, 2687, 2960, 2990
AsuC2I CCSGG 1 cut(s) 1652
AsuII TTCGAA 4 cut(s) 420, 624, 822, 3327
AvaI CYCGRG 1 cut(s) 1873
AvaII GGWCC 5 cut(s) 125, 2438, 2687, 2960, 2990
BaeGI GKGCMC 2 cut(s) 757, 1237
BalI TGGCCA 2 cut(s) 506, 2376
BbsI GAAGAC 5 cut(s) 357, 1416, 1437, 3108, 3170
Bbv12I GWGCWC 3 cut(s) 612, 757, 3125
BbvI GCAGC 7 cut(s) 475, 586, 1283, 2501, 3036, 3039, 3390
BccI CCATC 9 cut(s) 572, 1038, 1556, 1604, 1679, 1701, 2149, 2393, 2722
BceAI ACGGC 3 cut(s) 680, 724, 1767
BciT130I CCWGG 2 cut(s) 2738, 2769
BciVI GTATCC 1 cut(s) 1648
BclI TGATCA 3 cut(s) 532, 1309, 2629
BcnI CCSGG 1 cut(s) 1652
BcoDI GTCTC 7 cut(s) 637, 869, 1856, 1949, 2135, 2839, 3174
BcuI ACTAGT 3 cut(s) 1762, 1913, 2866
BfaI CTAG 6 cut(s) 897, 1536, 1763, 1914, 2501, 2867
BfmI CTRYAG 3 cut(s) 170, 600, 2004
BfrI CTTAAG 1 cut(s) 244
BfuAI ACCTGC 1 cut(s) 604
BfuI GTATCC 1 cut(s) 1648
BglII AGATCT 2 cut(s) 208, 1249
BisI GCNGC 9 cut(s) 45, 48, 489, 600, 1272, 2515, 3050, 3053, 3379
BlsI GCNGC 9 cut(s) 46, 49, 490, 601, 1273, 2516, 3051, 3054, 3380
BmcAI AGTACT 1 cut(s) 2645
Bme1390I CCNGG 3 cut(s) 1652, 2738, 2769
Bme18I GGWCC 5 cut(s) 125, 2438, 2687, 2960, 2990
BmeRI GACNNNNNGTC 1 cut(s) 1375
BmeT110I CYCGRG 1 cut(s) 1873
BmgT120I GGNCC 6 cut(s) 125, 670, 2438, 2687, 2960, 2990
BmiI GGNNCC 3 cut(s) 126, 2961, 3338
BmrFI CCNGG 3 cut(s) 1652, 2738, 2769
BmrI ACTGGG 2 cut(s) 2218, 3383
BmuI ACTGGG 2 cut(s) 2218, 3383
BpiI GAAGAC 5 cut(s) 357, 1416, 1437, 3108, 3170
BpmI CTGGAG 4 cut(s) 1799, 2210, 2759, 2879
Bpu14I TTCGAA 4 cut(s) 420, 624, 822, 3327
BpuEI CTTGAG 2 cut(s) 2555, 2771
BpuMI CCSGG 1 cut(s) 1652
BsaBI GATNNNNATC 2 cut(s) 207, 1248
BsaHI GRCGYC 2 cut(s) 720, 3167
BsaI GGTCTC 2 cut(s) 1856, 2135
BsaJI CCNNGG 7 cut(s) 651, 735, 1062, 1131, 1618, 2551, 2768
BsaWI WCCGGW 1 cut(s) 1440
BsaXI ACNNNNNCTCC 2 cut(s) 2732, 2762
Bsc4I CCNNNNNNNGG 5 cut(s) 51, 2100, 2250, 2383, 3045
Bse1I ACTGG 4 cut(s) 2213, 2690, 3340, 3389
Bse3DI GCAATG 2 cut(s) 485, 1209
Bse8I GATNNNNATC 2 cut(s) 207, 1248
BseBI CCWGG 2 cut(s) 2738, 2769
BseDI CCNNGG 7 cut(s) 651, 735, 1062, 1131, 1618, 2551, 2768
BseGI GGATG 8 cut(s) 205, 661, 1419, 1556, 1712, 2319, 2529, 2912
BseJI GATNNNNATC 2 cut(s) 207, 1248
BseLI CCNNNNNNNGG 5 cut(s) 51, 2100, 2250, 2383, 3045
BseMI GCAATG 2 cut(s) 485, 1209
BseMII CTCAG 5 cut(s) 47, 1102, 2930, 3072, 3416
BseNI ACTGG 4 cut(s) 2213, 2690, 3340, 3389
BseRI GAGGAG 6 cut(s) 190, 427, 887, 2250, 2714, 2756
BseSI GKGCMC 2 cut(s) 757, 1237
BseXI GCAGC 7 cut(s) 475, 586, 1283, 2501, 3036, 3039, 3390
BseYI CCCAGC 1 cut(s) 989
Bsh1236I CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 5 cut(s) 44, 506, 671, 2339, 2376
BsiEI CGRYCG 1 cut(s) 166
BsiHKAI GWGCWC 3 cut(s) 612, 757, 3125
BsiHKCI CYCGRG 1 cut(s) 1873
BsiSI CCGG 2 cut(s) 1441, 1651
BslFI GGGAC 2 cut(s) 1863, 2241
BslI CCNNNNNNNGG 5 cut(s) 51, 2100, 2250, 2383, 3045
BsmAI GTCTC 7 cut(s) 637, 869, 1856, 1949, 2135, 2839, 3174
BsmBI CGTCTC 1 cut(s) 3174
BsmFI GGGAC 2 cut(s) 1863, 2241
BsmI GAATGC 2 cut(s) 191, 1199
BsnI GGCC 5 cut(s) 44, 506, 671, 2339, 2376
Bso31I GGTCTC 2 cut(s) 1856, 2135
BsoBI CYCGRG 1 cut(s) 1873
Bsp119I TTCGAA 4 cut(s) 420, 624, 822, 3327
Bsp1286I GDGCHC 4 cut(s) 612, 757, 1237, 3125
Bsp1407I TGTACA 1 cut(s) 3414
Bsp19I CCATGG 4 cut(s) 651, 735, 1062, 2551
BspACI CCGC 6 cut(s) 45, 48, 52, 1197, 2477, 3118
BspANI GGCC 5 cut(s) 44, 506, 671, 2339, 2376
BspCNI CTCAG 5 cut(s) 46, 1101, 2931, 3073, 3417
BspFNI CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 3 cut(s) 126, 2961, 3338
BspMAI CTGCAG 1 cut(s) 604
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 4 cut(s) 197, 1849, 1908, 3398
BspQI GCTCTTC 1 cut(s) 2969
BspT104I TTCGAA 4 cut(s) 420, 624, 822, 3327
BspTI CTTAAG 1 cut(s) 244
BspTNI GGTCTC 2 cut(s) 1856, 2135
BsrDI GCAATG 2 cut(s) 485, 1209
BsrGI TGTACA 1 cut(s) 3414
BsrI ACTGG 4 cut(s) 2213, 2690, 3340, 3389
BssECI CCNNGG 7 cut(s) 651, 735, 1062, 1131, 1618, 2551, 2768
BssNI GRCGYC 2 cut(s) 720, 3167
BssT1I CCWWGG 6 cut(s) 651, 735, 1062, 1131, 1618, 2551
Bst2UI CCWGG 2 cut(s) 2738, 2769
Bst4CI ACNGT 9 cut(s) 151, 584, 1513, 2129, 2584, 3044, 3235, 3414, 3419
Bst6I CTCTTC 5 cut(s) 404, 1788, 2703, 2969, 3320
BstACI GRCGYC 2 cut(s) 720, 3167
BstAFI CTTAAG 1 cut(s) 244
BstAUI TGTACA 1 cut(s) 3414
BstBI TTCGAA 4 cut(s) 420, 624, 822, 3327
BstC8I GCNNGC 3 cut(s) 16, 763, 1541
BstDSI CCRYGG 4 cut(s) 651, 735, 1062, 2551
BstF5I GGATG 8 cut(s) 205, 661, 1419, 1556, 1712, 2319, 2529, 2912
BstFNI CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
BstHHI GCGC 3 cut(s) 52, 1985, 2886
BstMAI GTCTC 7 cut(s) 637, 869, 1856, 1949, 2135, 2839, 3174
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 3 cut(s) 56, 717, 1220
BstNI CCWGG 2 cut(s) 2738, 2769
BstNSI RCATGY 2 cut(s) 749, 2188
BstSCI CCNGG 3 cut(s) 1650, 2736, 2767
BstSFI CTRYAG 3 cut(s) 170, 600, 2004
BstSLI GKGCMC 2 cut(s) 757, 1237
BstUI CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
BstV1I GCAGC 7 cut(s) 475, 586, 1283, 2501, 3036, 3039, 3390
BstV2I GAAGAC 5 cut(s) 357, 1416, 1437, 3108, 3170
BstX2I RGATCY 4 cut(s) 208, 1249, 1854, 1900
BstYI RGATCY 4 cut(s) 208, 1249, 1854, 1900
BsuI GTATCC 1 cut(s) 1648
BsuRI GGCC 5 cut(s) 44, 506, 671, 2339, 2376
BtgI CCRYGG 4 cut(s) 651, 735, 1062, 2551
BtgZI GCGATG 1 cut(s) 1086
BtsCI GGATG 8 cut(s) 205, 661, 1419, 1556, 1712, 2319, 2529, 2912
BtsI GCAGTG 1 cut(s) 3156
BtsIMutI CAGTG 3 cut(s) 1518, 2976, 3156
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 3 cut(s) 16, 763, 1541
CciI TCATGA 1 cut(s) 1312
CfoI GCGC 3 cut(s) 52, 1985, 2886
Cfr13I GGNCC 6 cut(s) 125, 670, 2438, 2687, 2960, 2990
CseI GACGC 3 cut(s) 20, 709, 845
Csp6I GTAC 7 cut(s) 706, 1554, 1584, 1969, 2451, 2644, 3415
CviQI GTAC 7 cut(s) 706, 1554, 1584, 1969, 2451, 2644, 3415
DraI TTTAAA 3 cut(s) 495, 1848, 2874
DrdI GACNNNNNNGTC 1 cut(s) 686
DriI GACNNNNNGTC 1 cut(s) 1375
DseDI GACNNNNNNGTC 1 cut(s) 686
EaeI YGGCCR 2 cut(s) 504, 2374
Eam1104I CTCTTC 5 cut(s) 404, 1788, 2703, 2969, 3320
Eam1105I GACNNNNNGTC 1 cut(s) 1375
EarI CTCTTC 5 cut(s) 404, 1788, 2703, 2969, 3320
Eco130I CCWWGG 6 cut(s) 651, 735, 1062, 1131, 1618, 2551
Eco31I GGTCTC 2 cut(s) 1856, 2135
Eco32I GATATC 2 cut(s) 404, 2154
Eco47I GGWCC 5 cut(s) 125, 2438, 2687, 2960, 2990
Eco57I CTGAAG 2 cut(s) 1274, 2039
Eco88I CYCGRG 1 cut(s) 1873
EcoO109I RGGNCCY 2 cut(s) 125, 670
EcoRII CCWGG 2 cut(s) 2736, 2767
EcoRV GATATC 2 cut(s) 404, 2154
EcoT14I CCWWGG 6 cut(s) 651, 735, 1062, 1131, 1618, 2551
EcoT22I ATGCAT 3 cut(s) 347, 1641, 2952
ErhI CCWWGG 6 cut(s) 651, 735, 1062, 1131, 1618, 2551
Esp3I CGTCTC 1 cut(s) 3174
FalI AAGNNNNNCTT 6 cut(s) 1129, 1161, 1367, 1399, 3093, 3125
FaqI GGGAC 2 cut(s) 1863, 2241
FauI CCCGC 2 cut(s) 45, 1190
FauNDI CATATG 4 cut(s) 2202, 2519, 2799, 2814
FbaI TGATCA 3 cut(s) 532, 1309, 2629
FblI GTMKAC 4 cut(s) 153, 579, 1786, 2197
Fnu4HI GCNGC 9 cut(s) 45, 48, 489, 600, 1272, 2515, 3050, 3053, 3379
FokI GGATG 8 cut(s) 212, 668, 1406, 1543, 1719, 2306, 2536, 2919
Fsp4HI GCNGC 9 cut(s) 45, 48, 489, 600, 1272, 2515, 3050, 3053, 3379
FspBI CTAG 6 cut(s) 897, 1536, 1763, 1914, 2501, 2867
GlaI GCGC 3 cut(s) 51, 1984, 2885
GluI GCNGC 9 cut(s) 45, 48, 489, 600, 1272, 2515, 3050, 3053, 3379
GsaI CCCAGC 1 cut(s) 993
GsuI CTGGAG 4 cut(s) 1799, 2210, 2759, 2879
HaeIII GGCC 5 cut(s) 44, 506, 671, 2339, 2376
HapII CCGG 2 cut(s) 1441, 1651
HgaI GACGC 3 cut(s) 20, 709, 845
HhaI GCGC 3 cut(s) 52, 1985, 2886
Hin1I GRCGYC 2 cut(s) 720, 3167
Hin6I GCGC 3 cut(s) 50, 1983, 2884
HinP1I GCGC 3 cut(s) 50, 1983, 2884
HincII GTYRAC 1 cut(s) 154
HindII GTYRAC 1 cut(s) 154
HindIII AAGCTT 1 cut(s) 2024
HpaII CCGG 2 cut(s) 1441, 1651
Hpy99I CGWCG 2 cut(s) 14, 725
HpyCH4III ACNGT 9 cut(s) 151, 584, 1513, 2129, 2584, 3044, 3235, 3414, 3419
HpyCH4IV ACGT 3 cut(s) 1627, 2295, 3167
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 717, 1220
HpySE526I ACGT 3 cut(s) 1627, 2295, 3167
Hsp92I GRCGYC 2 cut(s) 720, 3167
HspAI GCGC 3 cut(s) 50, 1983, 2884
Ksp22I TGATCA 3 cut(s) 532, 1309, 2629
LguI GCTCTTC 1 cut(s) 2969
Lsp1109I GCAGC 7 cut(s) 475, 586, 1283, 2501, 3036, 3039, 3390
MaeI CTAG 6 cut(s) 897, 1536, 1763, 1914, 2501, 2867
MaeII ACGT 3 cut(s) 1627, 2295, 3167
MaeIII GTNAC 6 cut(s) 661, 1574, 2479, 2528, 2559, 2760
MfeI CAATTG 2 cut(s) 1449, 3095
MflI RGATCY 4 cut(s) 208, 1249, 1854, 1900
MhlI GDGCHC 4 cut(s) 612, 757, 1237, 3125
MlsI TGGCCA 2 cut(s) 506, 2376
MluI ACGCGT 1 cut(s) 3001
MluNI TGGCCA 2 cut(s) 506, 2376
MlyI GAGTC 6 cut(s) 535, 554, 1560, 1623, 1885, 3025
MmeI TCCRAC 7 cut(s) 33, 260, 849, 2685, 3063, 3111, 3418
Mox20I TGGCCA 2 cut(s) 506, 2376
Mph1103I ATGCAT 3 cut(s) 347, 1641, 2952
MroXI GAANNNNTTC 3 cut(s) 630, 828, 3105
MscI TGGCCA 2 cut(s) 506, 2376
MslI CAYNNNNRTG 4 cut(s) 750, 2522, 2537, 2637
Msp20I TGGCCA 2 cut(s) 506, 2376
MspA1I CMGCKG 1 cut(s) 86
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 2 cut(s) 1441, 1651
MspR9I CCNGG 3 cut(s) 1652, 2738, 2769
MunI CAATTG 2 cut(s) 1449, 3095
Mva1269I GAATGC 2 cut(s) 191, 1199
MvaI CCWGG 2 cut(s) 2738, 2769
MvnI CGCG 5 cut(s) 50, 52, 2477, 2886, 3003
MwoI GCNNNNNNNGC 3 cut(s) 56, 717, 1220
NciI CCSGG 1 cut(s) 1652
NcoI CCATGG 4 cut(s) 651, 735, 1062, 2551
NdeI CATATG 4 cut(s) 2202, 2519, 2799, 2814
NlaIV GGNNCC 3 cut(s) 126, 2961, 3338
NmeAIII GCCGAG 1 cut(s) 2097
NmuCI GTSAC 3 cut(s) 1574, 2479, 2528
NsiI ATGCAT 3 cut(s) 347, 1641, 2952
NspI RCATGY 2 cut(s) 749, 2188
NspV TTCGAA 4 cut(s) 420, 624, 822, 3327
PagI TCATGA 1 cut(s) 1312
PciI ACATGT 1 cut(s) 745
PciSI GCTCTTC 1 cut(s) 2969
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 564
PctI GAATGC 2 cut(s) 191, 1199
PdmI GAANNNNTTC 3 cut(s) 630, 828, 3105
PfeI GAWTC 9 cut(s) 260, 461, 572, 985, 1151, 1500, 1532, 2930, 3202
PfoI TCCNGGA 1 cut(s) 1650
PkrI GCNGC 9 cut(s) 46, 49, 490, 601, 1273, 2516, 3051, 3054, 3380
Ple19I CGATCG 1 cut(s) 166
PleI GAGTC 6 cut(s) 535, 554, 1560, 1622, 1884, 3024
PpsI GAGTC 6 cut(s) 535, 554, 1560, 1622, 1884, 3024
PpuMI RGGWCCY 1 cut(s) 125
PscI ACATGT 1 cut(s) 745
PshBI ATTAAT 1 cut(s) 1889
PsiI TTATAA 1 cut(s) 938
Psp5II RGGWCCY 1 cut(s) 125
Psp6I CCWGG 2 cut(s) 2736, 2767
PspFI CCCAGC 1 cut(s) 989
PspGI CCWGG 2 cut(s) 2736, 2767
PspN4I GGNNCC 3 cut(s) 126, 2961, 3338
PspPI GGNCC 6 cut(s) 125, 670, 2438, 2687, 2960, 2990
PspPPI RGGWCCY 1 cut(s) 125
PstI CTGCAG 1 cut(s) 604
PsuI RGATCY 4 cut(s) 208, 1249, 1854, 1900
PvuI CGATCG 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 7 cut(s) 707, 1555, 1585, 1970, 2452, 2645, 3416
RsaNI GTAC 7 cut(s) 706, 1554, 1584, 1969, 2451, 2644, 3415
RseI CAYNNNNRTG 4 cut(s) 750, 2522, 2537, 2637
SalI GTCGAC 1 cut(s) 152
SapI GCTCTTC 1 cut(s) 2969
SatI GCNGC 9 cut(s) 45, 48, 489, 600, 1272, 2515, 3050, 3053, 3379
Sau96I GGNCC 6 cut(s) 125, 670, 2438, 2687, 2960, 2990
ScaI AGTACT 1 cut(s) 2645
SchI GAGTC 6 cut(s) 535, 554, 1560, 1623, 1885, 3025
ScrFI CCNGG 3 cut(s) 1652, 2738, 2769
SduI GDGCHC 4 cut(s) 612, 757, 1237, 3125
SfcI CTRYAG 3 cut(s) 170, 600, 2004
SfuI TTCGAA 4 cut(s) 420, 624, 822, 3327
SinI GGWCC 5 cut(s) 125, 2438, 2687, 2960, 2990
SmiI ATTTAAAT 1 cut(s) 1848
SmiMI CAYNNNNRTG 4 cut(s) 750, 2522, 2537, 2637
SmlI CTYRAG 3 cut(s) 244, 2570, 2786
SmoI CTYRAG 3 cut(s) 244, 2570, 2786
SpeI ACTAGT 3 cut(s) 1762, 1913, 2866
SsiI CCGC 6 cut(s) 45, 48, 52, 1197, 2477, 3118
SspI AATATT 1 cut(s) 2248
SspMI CTAG 6 cut(s) 897, 1536, 1763, 1914, 2501, 2867
StyD4I CCNGG 3 cut(s) 1650, 2736, 2767
StyI CCWWGG 6 cut(s) 651, 735, 1062, 1131, 1618, 2551
SwaI ATTTAAAT 1 cut(s) 1848
TaaI ACNGT 9 cut(s) 151, 584, 1513, 2129, 2584, 3044, 3235, 3414, 3419
TaiI ACGT 3 cut(s) 1630, 2298, 3170
TaqII GACCGA 1 cut(s) 2009
TatI WGTACW 4 cut(s) 1553, 1968, 2643, 3414
TauI GCSGC 2 cut(s) 47, 50
TfiI GAWTC 9 cut(s) 260, 461, 572, 985, 1151, 1500, 1532, 2930, 3202
TscAI CASTG 3 cut(s) 1518, 2976, 3156
TseFI GTSAC 3 cut(s) 1574, 2479, 2528
TseI GCWGC 7 cut(s) 488, 599, 1271, 2514, 3049, 3052, 3378
Tsp45I GTSAC 3 cut(s) 1574, 2479, 2528
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 3 cut(s) 1518, 2976, 3156
Vha464I CTTAAG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 753
VpaK11BI GGWCC 5 cut(s) 125, 2438, 2687, 2960, 2990
VspI ATTAAT 1 cut(s) 1889
XapI RAATTY 5 cut(s) 1080, 2031, 2086, 2387, 2965
XceI RCATGY 2 cut(s) 749, 2188
XmiI GTMKAC 4 cut(s) 153, 579, 1786, 2197
XmnI GAANNNNTTC 3 cut(s) 630, 828, 3105
XspI CTAG 6 cut(s) 897, 1536, 1763, 1914, 2501, 2867
ZraI GACGTC 1 cut(s) 3168
ZrmI AGTACT 1 cut(s) 2645
Zsp2I ATGCAT 3 cut(s) 347, 1641, 2952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.