Rroxscaffold_1G00003210

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000379
Physical Location & Seq
Forward (+)
3755432 .. 3762900
7469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003210.1

Sequence Viewer

Length: 3597 bp
ATGGAGTTCTATTTCTTCCAGATGTTGTTGGCGATCGTTGTGGTAATTGTGACATGGATCGGTGCCATAGCTACTATTAAGTGGATATGGACTCTACTGAAGCATTGGTTCCGTTACATGGTTGAAAGGCGTGAACCAATATCTAAGGATAATCAAGAATTGATCGACATCGCTACTGAGCCCGAGAGAGAAGAAGAAGAAGAAAAGATGTGGGATCTCATTGACTCTCCTGATCAGGGAAACGATGGCACCACCTCGGCCGCCGCACCGGTTGCTTCTTCTTCTTCTTCTCTATCATCAGCTGTTGAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATCACATTTGAACTATACGATCGACTGCAGAGGAGGGGAATTAAAGCATTCATGGATGATCGAGATCTTGAAGTAGGGGATGTTATTTCTCCAACTCTCTTAAGAGCAATTAGAGAATCAAGGTTTGCAATCGTTATTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACGACAGTAGAATCCTGCCACTCTTTTATCAGGTTGATCCAACTGATGTTCGATATCAGAAGAGGAGTTTTGAAGAAGCTTTCACTAGGCATGAAACCTCTGGGCGACATGAATCAGAGAAGGTGGAAAAGTGGAAAGCTGCTCTAAACAAAGTGGCCAATATCTCTGGGTGGAATACAAATGATCATAAGACTCACCAAGAACTGATCGACTCCATTGTGGAATCTGTCTCCTGTAAAGTCTTACCTGCTGCAGTTGAGCCCATGGGAGAGTTCAAAGAGTTTCAAGCAACGAGACAAGCCATGAATGAAGTTATGCAGGCGCTAAAAGATGATGAAGTCAATGCCGTTGGCGTGTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGGTGCACAAGCCTGCAAAAATGGGATTTTTGATCGTGTGATTATGGCTGTCGTTTCACAAAATCCTAATTTCGAGAAAATTCAAGGCACATTGGCAGAGCAGTTGGGCTTCAAATTGTGGGAGGAGACAGAAATTGGACGAATTGCTAGATTGAATAAGGAGATAATGAGAAGAGAAAAACTCCTTATAATCTTGGACGATGTTTGGGGGAGAGCAGAGTTATCAAATATAGGGATTCCCATCTACAAGGAACTTCAAAAGTGCAAGTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCATGCCATGGAGTGCCAAAAAAGGGTCACCCTCACTGTCCTCTCAAAAGAAGATTCTTGGACTTTGTTTGTGACAAAAGCAAGAAGGTCTTTTGAGTCCGCCAAATTTGAGGATGTGGCAAGGAGGGTAGCTGGAGAATGTAGGGGTCTACCGATAGCATTGATACCAGTTGCAAGGGCACTTGGAGATAAAGACCTAGCGGAATGGGAAAAAGCAGCTCAAAGACTAGAGAAGTCGCAAATTGCCAACCCTGACCATGATGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAACATGAAGACCACAAGTCATGCTTCCTGCTCTGTTGCCTATACCCAGAAGACCATGACATTCGACTAGAAAACTTGTTCAAGTATGCAATCGGGAAAGGATTGTTTCGAGATGCAGAAACAATCGAAGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTTAAAGATTCTAGCTTGCTTTTGGATAGCGAGAAAAAAGGATGTGTAAAGATGCATGATGTCATCAGGGATACTGCCCTGAATATTGCAAAATCTAAAGATGGGCACGGGTTTCTGGTAAAAGCGGGCTGTGGTATAAAGGATTGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAGAATTCGCAAGCTACCCGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAAACGGAATGATGATTTGAACAATATCCCAGAGAAGTTTGTCCAAAGTCTGAATGAATTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCTTACTACCCCAATCATTCAGTCTCCTTACCAATCTCCAAGCTTTGTATTTAGATTTTTGCTGGAAATTGACTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGAGAAAATCGTCTTAGGAAATTGTCAAGAGAAATAGGAAATTTGACCAATCTAAGGATGTTGGATATCCGTGCCGACAAACCAGTAGCTGGAGGTGTTATCACAATTCCTGCTGGAGTGATATCGAAGTTGCATAGATTAGAAGAACTGTACATGATGTACTGTGTATTTGAGGACTTGGATCAGGAAGAAGAAACCAATATTGGGTTTGATGAGTTAGCTGGTTTATCAAATTTGCTAATTTTGCACGTTTGCATATCTGATGTAAAATACATACCTAAAGATGTTGAGGTTCCGAATTGGGTTTACTTTGATATACGTATCAACAAAGGCTCATGGGATCCATACGATCAACAAGATCATAATTCAAGAACCTTGGTTCTTCGTTTCACAACCATCAGTACCTTACCAGATTGGTTTGTGAACGCGGTGACAACGAAAACAGAGAAGCTAGCGTACGACTGCTACCAAGGGATGAGTGACATTCTTATGGAATATGACCACGGGAGGTTGCATGAACTAAGGCATCTCGTTGTATCTGGTTCTCTTTTTGGTTCCTCTAAGTACTTAGAAGAGTTGATGAACACAAGAAGACGGGTTCAAAAAGGACCAGTATTTGAGAACTTAGAAGAGTTGCATCTGATACATCTGATCCACCTTAATGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCATTAATCTTAAGGTGTTTCATGTGTTTCGTTGTATGAAATTGGGGAACGTATCAAAGTTGGTACAGAAACTACCAAATCTGGAGAAACTTTATTTAAACTGTTTGCGTAGATTGGAATATGTGTTTGGATGTGAAGCAGATCAGGCAGCACAATCAAAACTGAGAGAGATATATTTGTTGGGTCCAAATTCAGTGAAAAGCATTTGTAATGGTCCCGTCCCACGTGTAATGTTCCAGAGTCTTAAAAGTTTGACCTTTTACCGTTGTGACGTGCTGCAAAGTCTCTTCGCATCTGATGTAGCTCAGTGTCTTGTTCAATTGGAAGACATTTTTGTACAACAGTGTCCTTTGTTGGAAAGGGTAATTGAAGCTGTGAACGACGAGAGACCGGTTCTTCCAAGATTGAAGAACTTGGTTTTGATGCAACTTCCTATGTTGTATGGTGCAAGTGCTACTGGTGAAATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGCCCCCAATTTTCATTTTCAATCTCTTCCTCTCTCTTCTTCACCTTCAGAAGCAAGAACCGGTTTTCATTTTCAACTTCTGCTTCTTGCTACTTTGGCAGCAGGAACATAATCAAACTCAATGATGAACAACTGTACAGCTTTCTACGTTACAGATCGGACCCTGTATCTCGTTTCCAAAATAAATGA

Protein Analysis

1198

Amino Acids

137.22

Weight (kDa)

6.17

Isoelectric Point (pI)

46.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 105 - 271 5.1e-49 TIR domain
TIR_2 PF13676 108 - 203 7.7e-16 TIR domain
NB-ARC PF00931 289 - 452 4.3e-33 NB-ARC domain
LRR_8 PF13855 658 - 715 3.5e-08 Leucine rich repeat
LRR_14 PF23598 663 - 841 1.2e-15 Leucine-rich repeat region
LRR_RPS2 PF23247 945 - 1084 5.1e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 1030 - 1145 2.6e-12 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1157
Acc36I ACCTGC 1 cut(s) 823
AccB1I GGYRCC 2 cut(s) 62, 248
AccB7I CCANNNNNTGG 1 cut(s) 2297
AccI GTMKAC 1 cut(s) 1423
AccII CGCG 2 cut(s) 1896, 2636
AciI CCGC 7 cut(s) 261, 264, 1374, 1475, 1868, 1896, 2636
AclWI GGATC 8 cut(s) 65, 222, 599, 2073, 2397, 2543, 2556, 2854
AcoI YGGCCR 3 cut(s) 258, 723, 1889
AcsI RAATTY 7 cut(s) 1047, 1379, 1941, 2196, 2248, 2440, 3094
AcuI CTGAAG 3 cut(s) 119, 2204, 3439
AcvI CACGTG 1 cut(s) 3131
AcyI GRCGYC 1 cut(s) 939
AfiI CCNNNNNNNGG 6 cut(s) 118, 236, 1297, 2262, 2297, 2412
AflII CTTAAG 2 cut(s) 460, 2915
AflIII ACRYGT 2 cut(s) 964, 3130
AgeI ACCGGT 3 cut(s) 268, 3295, 3468
AhdI GACNNNNNGTC 1 cut(s) 1594
AhlI ACTAGT 1 cut(s) 2078
AjiI CACGTC 1 cut(s) 3178
AjnI CCWGG 1 cut(s) 2896
AjuI GAANNNNNNNTTGG 4 cut(s) 2394, 2426, 3015, 3047
Alw21I GWGCWC 1 cut(s) 976
Alw26I GTCTC 7 cut(s) 802, 856, 1088, 2114, 2907, 3194, 3286
Alw44I GTGCAC 1 cut(s) 972
AlwI GGATC 8 cut(s) 65, 222, 599, 2073, 2397, 2543, 2556, 2854
AlwNI CAGNNNCTG 1 cut(s) 2297
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 258, 723, 1889
ApaLI GTGCAC 1 cut(s) 972
ApeKI GCWGC 7 cut(s) 707, 818, 1490, 1911, 3053, 3181, 3507
ApoI RAATTY 7 cut(s) 1047, 1379, 1941, 2196, 2248, 2440, 3094
AseI ATTAAT 1 cut(s) 2910
AsiGI ACCGGT 3 cut(s) 268, 3295, 3468
AspLEI GCGC 1 cut(s) 892
AspS9I GGNCC 5 cut(s) 341, 2816, 3089, 3119, 3568
AsuHPI GGTGA 7 cut(s) 755, 1243, 1294, 2650, 2897, 3377, 3442
AsuNHI GCTAGC 1 cut(s) 2659
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 5 cut(s) 341, 2816, 3089, 3119, 3568
BaeGI GKGCMC 3 cut(s) 976, 1456, 1851
BalI TGGCCA 2 cut(s) 725, 1891
BamHI GGATCC 1 cut(s) 2548
BanI GGYRCC 2 cut(s) 62, 248
BanII GRGCYC 2 cut(s) 183, 831
BarI GAAGNNNNNNTAC 2 cut(s) 2648, 2680
BbrPI CACGTG 1 cut(s) 3131
BbsI GAAGAC 5 cut(s) 573, 1593, 1635, 2806, 3237
Bbv12I GWGCWC 1 cut(s) 976
BbvI GCAGC 7 cut(s) 694, 805, 1502, 1898, 3065, 3168, 3519
BccI CCATC 4 cut(s) 239, 1217, 1838, 2612
BceAI ACGGC 2 cut(s) 899, 943
BciT130I CCWGG 1 cut(s) 2898
BciVI GTATCC 1 cut(s) 1807
BclI TGATCA 2 cut(s) 232, 751
BcoDI GTCTC 7 cut(s) 802, 856, 1088, 2114, 2907, 3194, 3286
BcuI ACTAGT 1 cut(s) 2078
BfaI CTAG 8 cut(s) 654, 1116, 1472, 1502, 1646, 1755, 2079, 2660
BfmI CTRYAG 3 cut(s) 386, 819, 2169
BfoI RGCGCY 1 cut(s) 893
BfrI CTTAAG 2 cut(s) 460, 2915
BfuAI ACCTGC 1 cut(s) 823
BfuI GTATCC 1 cut(s) 1807
BglII AGATCT 1 cut(s) 424
BisI GCNGC 9 cut(s) 261, 264, 708, 819, 1491, 1912, 3054, 3182, 3508
BlsI GCNGC 9 cut(s) 262, 265, 709, 820, 1492, 1913, 3055, 3183, 3509
BmcAI AGTACT 1 cut(s) 2774
Bme1390I CCNGG 1 cut(s) 2898
Bme18I GGWCC 5 cut(s) 341, 2816, 3089, 3119, 3568
BmeRI GACNNNNNGTC 1 cut(s) 1594
BmeT110I CYCGRG 1 cut(s) 182
BmgBI CACGTC 1 cut(s) 3178
BmgT120I GGNCC 5 cut(s) 341, 2816, 3089, 3119, 3568
BmrFI CCNGG 1 cut(s) 2898
BmsI GCATC 7 cut(s) 1531, 1681, 1785, 2743, 2854, 3206, 3318
BmtI GCTAGC 1 cut(s) 2663
BpiI GAAGAC 5 cut(s) 573, 1593, 1635, 2806, 3237
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 5 cut(s) 1428, 1964, 2319, 2343, 3008
BsaAI YACGTR 2 cut(s) 2528, 3131
BsaBI GATNNNNATC 3 cut(s) 167, 423, 1208
BsaHI GRCGYC 1 cut(s) 939
BsaI GGTCTC 2 cut(s) 2907, 3286
BsaJI CCNNGG 8 cut(s) 255, 831, 954, 1281, 2583, 2677, 2710, 2897
BsaWI WCCGGW 3 cut(s) 268, 3295, 3468
Bsc4I CCNNNNNNNGG 6 cut(s) 118, 236, 1297, 2262, 2297, 2412
Bse118I RCCGGY 3 cut(s) 268, 3295, 3468
Bse1I ACTGG 4 cut(s) 1442, 2291, 2819, 3367
Bse8I GATNNNNATC 3 cut(s) 167, 423, 1208
BseBI CCWGG 1 cut(s) 2898
BseDI CCNNGG 8 cut(s) 255, 831, 954, 1281, 2583, 2677, 2710, 2897
BseGI GGATG 7 cut(s) 421, 445, 1393, 1790, 2271, 2688, 3041
BseJI GATNNNNATC 3 cut(s) 167, 423, 1208
BseLI CCNNNNNNNGG 6 cut(s) 118, 236, 1297, 2262, 2297, 2412
BseMII CTCAG 3 cut(s) 168, 3059, 3224
BseNI ACTGG 4 cut(s) 1442, 2291, 2819, 3367
BseRI GAGGAG 3 cut(s) 406, 646, 1106
BseSI GKGCMC 3 cut(s) 976, 1456, 1851
BseX3I CGGCCG 1 cut(s) 258
BseXI GCAGC 7 cut(s) 694, 805, 1502, 1898, 3065, 3168, 3519
BsgI GTGCAG 1 cut(s) 1897
Bsh1236I CGCG 2 cut(s) 1896, 2636
Bsh1285I CGRYCG 3 cut(s) 36, 261, 382
BshFI GGCC 3 cut(s) 260, 725, 1891
BshNI GGYRCC 2 cut(s) 62, 248
BshTI ACCGGT 3 cut(s) 268, 3295, 3468
BsiEI CGRYCG 3 cut(s) 36, 261, 382
BsiHKAI GWGCWC 1 cut(s) 976
BsiHKCI CYCGRG 1 cut(s) 182
BsiSI CCGG 3 cut(s) 269, 3296, 3469
BsiWI CGTACG 1 cut(s) 2664
BslFI GGGAC 2 cut(s) 3105, 3110
BslI CCNNNNNNNGG 6 cut(s) 118, 236, 1297, 2262, 2297, 2412
BsmAI GTCTC 7 cut(s) 802, 856, 1088, 2114, 2907, 3194, 3286
BsmFI GGGAC 2 cut(s) 3105, 3110
BsmI GAATGC 2 cut(s) 407, 1544
BsnI GGCC 3 cut(s) 260, 725, 1891
Bso31I GGTCTC 2 cut(s) 2907, 3286
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 5 cut(s) 183, 831, 976, 1456, 1851
Bsp1407I TGTACA 3 cut(s) 2358, 3241, 3543
Bsp19I CCATGG 3 cut(s) 831, 954, 1281
BspACI CCGC 7 cut(s) 261, 264, 1374, 1475, 1868, 1896, 2636
BspANI GGCC 3 cut(s) 260, 725, 1891
BspCNI CTCAG 3 cut(s) 169, 3060, 3223
BspFNI CGCG 2 cut(s) 1896, 2636
BspMAI CTGCAG 2 cut(s) 390, 823
BspMI ACCTGC 1 cut(s) 823
BspOI GCTAGC 1 cut(s) 2663
BspPI GGATC 8 cut(s) 65, 222, 599, 2073, 2397, 2543, 2556, 2854
BspT107I GGYRCC 2 cut(s) 62, 248
BspTI CTTAAG 2 cut(s) 460, 2915
BspTNI GGTCTC 2 cut(s) 2907, 3286
BsrFI RCCGGY 3 cut(s) 268, 3295, 3468
BsrGI TGTACA 3 cut(s) 2358, 3241, 3543
BsrI ACTGG 4 cut(s) 1442, 2291, 2819, 3367
BssAI RCCGGY 3 cut(s) 268, 3295, 3468
BssECI CCNNGG 8 cut(s) 255, 831, 954, 1281, 2583, 2677, 2710, 2897
BssNI GRCGYC 1 cut(s) 939
BssT1I CCWWGG 5 cut(s) 831, 954, 1281, 2583, 2677
Bst2UI CCWGG 1 cut(s) 2898
Bst4CI ACNGT 8 cut(s) 575, 1312, 2358, 2372, 3008, 3170, 3249, 3543
Bst6I CTCTTC 8 cut(s) 623, 1135, 1953, 2775, 2832, 3197, 3439, 3449
BstACI GRCGYC 1 cut(s) 939
BstAFI CTTAAG 2 cut(s) 460, 2915
BstAPI GCANNNNNTGC 1 cut(s) 272
BstAUI TGTACA 3 cut(s) 2358, 3241, 3543
BstBAI YACGTR 2 cut(s) 2528, 3131
BstC8I GCNNGC 6 cut(s) 888, 982, 1760, 1870, 1949, 2661
BstDSI CCRYGG 4 cut(s) 831, 954, 1281, 2710
BstEII GGTNACC 1 cut(s) 1300
BstF5I GGATG 7 cut(s) 421, 445, 1393, 1790, 2271, 2688, 3041
BstFNI CGCG 2 cut(s) 1896, 2636
BstH2I RGCGCY 1 cut(s) 893
BstHHI GCGC 1 cut(s) 892
BstMAI GTCTC 7 cut(s) 802, 856, 1088, 2114, 2907, 3194, 3286
BstMCI CGRYCG 3 cut(s) 36, 261, 382
BstMWI GCNNNNNNNGC 6 cut(s) 272, 927, 936, 2452, 3050, 3504
BstNI CCWGG 1 cut(s) 2898
BstNSI RCATGY 1 cut(s) 968
BstPI GGTNACC 1 cut(s) 1300
BstSCI CCNGG 1 cut(s) 2896
BstSFI CTRYAG 3 cut(s) 386, 819, 2169
BstSLI GKGCMC 3 cut(s) 976, 1456, 1851
BstSNI TACGTA 1 cut(s) 2528
BstUI CGCG 2 cut(s) 1896, 2636
BstV1I GCAGC 7 cut(s) 694, 805, 1502, 1898, 3065, 3168, 3519
BstV2I GAAGAC 5 cut(s) 573, 1593, 1635, 2806, 3237
BstX2I RGATCY 4 cut(s) 214, 424, 2065, 2548
BstYI RGATCY 4 cut(s) 214, 424, 2065, 2548
BstZI CGGCCG 1 cut(s) 258
BsuI GTATCC 1 cut(s) 1807
BsuRI GGCC 3 cut(s) 260, 725, 1891
BtgI CCRYGG 4 cut(s) 831, 954, 1281, 2710
BtgZI GCGATG 1 cut(s) 154
BtrI CACGTC 1 cut(s) 3178
BtsCI GGATG 7 cut(s) 421, 445, 1393, 1790, 2271, 2688, 3041
BtsI GCAGTG 1 cut(s) 1914
BtsIMutI CAGTG 6 cut(s) 1308, 1737, 1914, 3105, 3218, 3254
BveI ACCTGC 1 cut(s) 823
Cac8I GCNNGC 6 cut(s) 888, 982, 1760, 1870, 1949, 2661
CaiI CAGNNNCTG 1 cut(s) 2297
CfoI GCGC 1 cut(s) 892
Cfr10I RCCGGY 3 cut(s) 268, 3295, 3468
Cfr13I GGNCC 5 cut(s) 341, 2816, 3089, 3119, 3568
CseI GACGC 1 cut(s) 928
CspAI ACCGGT 3 cut(s) 268, 3295, 3468
DraI TTTAAA 1 cut(s) 3003
DriI GACNNNNNGTC 1 cut(s) 1594
EaeI YGGCCR 3 cut(s) 258, 723, 1889
EagI CGGCCG 1 cut(s) 258
Eam1104I CTCTTC 8 cut(s) 623, 1135, 1953, 2775, 2832, 3197, 3439, 3449
Eam1105I GACNNNNNGTC 1 cut(s) 1594
EarI CTCTTC 8 cut(s) 623, 1135, 1953, 2775, 2832, 3197, 3439, 3449
EciI GGCGGA 1 cut(s) 1363
EclXI CGGCCG 1 cut(s) 258
Eco105I TACGTA 1 cut(s) 2528
Eco130I CCWWGG 5 cut(s) 831, 954, 1281, 2583, 2677
Eco24I GRGCYC 2 cut(s) 183, 831
Eco31I GGTCTC 2 cut(s) 2907, 3286
Eco32I GATATC 3 cut(s) 623, 2275, 2331
Eco47I GGWCC 5 cut(s) 341, 2816, 3089, 3119, 3568
Eco52I CGGCCG 1 cut(s) 258
Eco57I CTGAAG 3 cut(s) 119, 2204, 3439
Eco72I CACGTG 1 cut(s) 3131
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 1300
EcoO109I RGGNCCY 1 cut(s) 341
EcoO65I GGTNACC 1 cut(s) 1300
EcoRI GAATTC 1 cut(s) 1941
EcoRII CCWGG 1 cut(s) 2896
EcoRV GATATC 3 cut(s) 623, 2275, 2331
EcoT14I CCWWGG 5 cut(s) 831, 954, 1281, 2583, 2677
EcoT22I ATGCAT 3 cut(s) 563, 1546, 1800
EcoT38I GRGCYC 2 cut(s) 183, 831
ErhI CCWWGG 5 cut(s) 831, 954, 1281, 2583, 2677
FalI AAGNNNNNCTT 4 cut(s) 1348, 1380, 1586, 1618
FaqI GGGAC 2 cut(s) 3105, 3110
FauI CCCGC 1 cut(s) 1861
FbaI TGATCA 2 cut(s) 232, 751
FblI GTMKAC 1 cut(s) 1423
Fnu4HI GCNGC 9 cut(s) 261, 264, 708, 819, 1491, 1912, 3054, 3182, 3508
FokI GGATG 7 cut(s) 428, 452, 1400, 1797, 2278, 2695, 3048
FriOI GRGCYC 2 cut(s) 183, 831
Fsp4HI GCNGC 9 cut(s) 261, 264, 708, 819, 1491, 1912, 3054, 3182, 3508
FspBI CTAG 8 cut(s) 654, 1116, 1472, 1502, 1646, 1755, 2079, 2660
GlaI GCGC 1 cut(s) 891
GluI GCNGC 9 cut(s) 261, 264, 708, 819, 1491, 1912, 3054, 3182, 3508
GsuI CTGGAG 5 cut(s) 1428, 1964, 2319, 2343, 3008
HaeII RGCGCY 1 cut(s) 893
HaeIII GGCC 3 cut(s) 260, 725, 1891
HapII CCGG 3 cut(s) 269, 3296, 3469
HgaI GACGC 1 cut(s) 928
HhaI GCGC 1 cut(s) 892
Hin1I GRCGYC 1 cut(s) 939
Hin6I GCGC 1 cut(s) 890
HinP1I GCGC 1 cut(s) 890
HindIII AAGCTT 3 cut(s) 645, 2127, 2189
HpaII CCGG 3 cut(s) 269, 3296, 3469
HphI GGTGA 7 cut(s) 755, 1243, 1294, 2650, 2897, 3377, 3442
Hpy166II GTNNAC 7 cut(s) 134, 925, 974, 1424, 2515, 2632, 3283
Hpy8I GTNNAC 7 cut(s) 134, 925, 974, 1424, 2515, 2632, 3283
Hpy99I CGWCG 2 cut(s) 944, 3290
HpyAV CCTTC 5 cut(s) 682, 1353, 1901, 3390, 3463
HpyCH4III ACNGT 8 cut(s) 575, 1312, 2358, 2372, 3008, 3170, 3249, 3543
HpyCH4IV ACGT 6 cut(s) 2457, 2527, 2955, 3130, 3177, 3556
HpyF10VI GCNNNNNNNGC 6 cut(s) 272, 927, 936, 2452, 3050, 3504
HpySE526I ACGT 6 cut(s) 2457, 2527, 2955, 3130, 3177, 3556
Hsp92I GRCGYC 1 cut(s) 939
HspAI GCGC 1 cut(s) 890
Ksp22I TGATCA 2 cut(s) 232, 751
Lsp1109I GCAGC 7 cut(s) 694, 805, 1502, 1898, 3065, 3168, 3519
LweI GCATC 7 cut(s) 1531, 1681, 1785, 2743, 2854, 3206, 3318
MaeI CTAG 8 cut(s) 654, 1116, 1472, 1502, 1646, 1755, 2079, 2660
MaeII ACGT 6 cut(s) 2457, 2527, 2955, 3130, 3177, 3556
MaeIII GTNAC 9 cut(s) 49, 113, 1300, 1345, 2638, 2687, 2889, 3173, 3557
MfeI CAATTG 1 cut(s) 3224
MflI RGATCY 4 cut(s) 214, 424, 2065, 2548
MhlI GDGCHC 5 cut(s) 183, 831, 976, 1456, 1851
MlsI TGGCCA 2 cut(s) 725, 1891
MluNI TGGCCA 2 cut(s) 725, 1891
MlyI GAGTC 6 cut(s) 85, 218, 754, 773, 1379, 3154
MmeI TCCRAC 4 cut(s) 476, 632, 2250, 3240
Mox20I TGGCCA 2 cut(s) 725, 1891
Mph1103I ATGCAT 3 cut(s) 563, 1546, 1800
MscI TGGCCA 2 cut(s) 725, 1891
MslI CAYNNNNRTG 4 cut(s) 969, 1549, 2696, 2868
Msp20I TGGCCA 2 cut(s) 725, 1891
MspA1I CMGCKG 1 cut(s) 302
MspCI CTTAAG 2 cut(s) 460, 2915
MspI CCGG 3 cut(s) 269, 3296, 3469
MspR9I CCNGG 1 cut(s) 2898
MunI CAATTG 1 cut(s) 3224
Mva1269I GAATGC 2 cut(s) 407, 1544
MvaI CCWGG 1 cut(s) 2898
MvnI CGCG 2 cut(s) 1896, 2636
MwoI GCNNNNNNNGC 6 cut(s) 272, 927, 936, 2452, 3050, 3504
NcoI CCATGG 3 cut(s) 831, 954, 1281
NheI GCTAGC 1 cut(s) 2659
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 6 cut(s) 49, 1300, 1345, 2638, 2687, 3173
NsiI ATGCAT 3 cut(s) 563, 1546, 1800
NspI RCATGY 1 cut(s) 968
PciI ACATGT 1 cut(s) 964
PctI GAATGC 2 cut(s) 407, 1544
PfeI GAWTC 8 cut(s) 476, 579, 680, 791, 1204, 1328, 1727, 1751
Pfl23II CGTACG 1 cut(s) 2664
PflMI CCANNNNNTGG 1 cut(s) 2297
PinAI ACCGGT 3 cut(s) 268, 3295, 3468
PkrI GCNGC 9 cut(s) 262, 265, 709, 820, 1492, 1913, 3055, 3183, 3509
Ple19I CGATCG 2 cut(s) 36, 382
PleI GAGTC 6 cut(s) 85, 218, 754, 773, 1378, 3153
PmaCI CACGTG 1 cut(s) 3131
PmlI CACGTG 1 cut(s) 3131
PpsI GAGTC 6 cut(s) 85, 218, 754, 773, 1378, 3153
Ppu21I YACGTR 2 cut(s) 2528, 3131
PpuMI RGGWCCY 1 cut(s) 341
PscI ACATGT 1 cut(s) 964
PshBI ATTAAT 1 cut(s) 2910
PsiI TTATAA 1 cut(s) 1157
Psp5II RGGWCCY 1 cut(s) 341
Psp6I CCWGG 1 cut(s) 2896
PspCI CACGTG 1 cut(s) 3131
PspEI GGTNACC 1 cut(s) 1300
PspGI CCWGG 1 cut(s) 2896
PspLI CGTACG 1 cut(s) 2664
PspPI GGNCC 5 cut(s) 341, 2816, 3089, 3119, 3568
PspPPI RGGWCCY 1 cut(s) 341
PstI CTGCAG 2 cut(s) 390, 823
PstNI CAGNNNCTG 1 cut(s) 2297
PsuI RGATCY 4 cut(s) 214, 424, 2065, 2548
PvuI CGATCG 2 cut(s) 36, 382
PvuII CAGCTG 1 cut(s) 302
RseI CAYNNNNRTG 4 cut(s) 969, 1549, 2696, 2868
SatI GCNGC 9 cut(s) 261, 264, 708, 819, 1491, 1912, 3054, 3182, 3508
Sau96I GGNCC 5 cut(s) 341, 2816, 3089, 3119, 3568
ScaI AGTACT 1 cut(s) 2774
SchI GAGTC 6 cut(s) 85, 218, 754, 773, 1379, 3154
ScrFI CCNGG 1 cut(s) 2898
SduI GDGCHC 5 cut(s) 183, 831, 976, 1456, 1851
SfaNI GCATC 7 cut(s) 1531, 1681, 1785, 2743, 2854, 3206, 3318
SfcI CTRYAG 3 cut(s) 386, 819, 2169
SinI GGWCC 5 cut(s) 341, 2816, 3089, 3119, 3568
SmiMI CAYNNNNRTG 4 cut(s) 969, 1549, 2696, 2868
SmlI CTYRAG 2 cut(s) 460, 2915
SmoI CTYRAG 2 cut(s) 460, 2915
SnaBI TACGTA 1 cut(s) 2528
SpeI ACTAGT 1 cut(s) 2078
SsiI CCGC 7 cut(s) 261, 264, 1374, 1475, 1868, 1896, 2636
SspI AATATT 2 cut(s) 1828, 2410
SspMI CTAG 8 cut(s) 654, 1116, 1472, 1502, 1646, 1755, 2079, 2660
StyD4I CCNGG 1 cut(s) 2896
StyI CCWWGG 5 cut(s) 831, 954, 1281, 2583, 2677
TaaI ACNGT 8 cut(s) 575, 1312, 2358, 2372, 3008, 3170, 3249, 3543
TaiI ACGT 6 cut(s) 2460, 2530, 2958, 3133, 3180, 3559
TatI WGTACW 5 cut(s) 2358, 2367, 2772, 3241, 3543
TauI GCSGC 2 cut(s) 263, 266
TfiI GAWTC 8 cut(s) 476, 579, 680, 791, 1204, 1328, 1727, 1751
TscAI CASTG 6 cut(s) 1315, 1737, 1921, 3105, 3218, 3254
TseFI GTSAC 6 cut(s) 49, 1300, 1345, 2638, 2687, 3173
TseI GCWGC 7 cut(s) 707, 818, 1490, 1911, 3053, 3181, 3507
Tsp45I GTSAC 6 cut(s) 49, 1300, 1345, 2638, 2687, 3173
TspGWI ACGGA 3 cut(s) 101, 2014, 2267
TspRI CASTG 6 cut(s) 1315, 1737, 1921, 3105, 3218, 3254
Van91I CCANNNNNTGG 1 cut(s) 2297
Vha464I CTTAAG 2 cut(s) 460, 2915
VneI GTGCAC 1 cut(s) 972
VpaK11BI GGWCC 5 cut(s) 341, 2816, 3089, 3119, 3568
VspI ATTAAT 1 cut(s) 2910
XapI RAATTY 7 cut(s) 1047, 1379, 1941, 2196, 2248, 2440, 3094
XceI RCATGY 1 cut(s) 968
XmiI GTMKAC 1 cut(s) 1423
XspI CTAG 8 cut(s) 654, 1116, 1472, 1502, 1646, 1755, 2079, 2660
ZrmI AGTACT 1 cut(s) 2774
Zsp2I ATGCAT 3 cut(s) 563, 1546, 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.