FvH4_3g42940

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Reverse (-)
35876570 .. 35881170
4601 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g42940.t1

Sequence Viewer

Length: 3396 bp
ATGGCCTCCCCTGCATCTCTTCCTTCATCTGCTGAATCACCAACCGCTTGTCCGTCAAATTACGATGTGTTTTTGAGTTTCAGTGGTCTTGACACTCGAAAAGGTATTACATCTGAATTATATGAGCGGCTGCATAAACAGAGAGGAATTATAACATTCATGGATGATCGAGATCTTGAACCAGGGGATCCTATATCTCGCACCCTACTAAAAGCGATTGAAGCATCAAGGTTTGCAATTGTTGTCCTTTCTCCAAACTATGCCTCTTCTACTTGGTGTTTGGAGGAACTTGCCAAGATCTGTGAGTGCATGAAAGACCAGAACAGAATTCTGCCGCTTTTCTATAAGGTGGAACCCTCTGATGTACGGCACCGGAAGGGGAGTTTCGGAGAAGCTTTCGATAAGCATAAAAGTTGTGGGCGACACACATCAGAACAGCTGGAGCAATGGAGTCTTGCTTTAAACAAAGTGGCCAGTTTCACTGGGTGGCACACAAAGAATTATAAGACTGATAGAGAACTTGTGGAGGAAATTGTGGATTCTGTGTGCAACAGAATACGACCTTTTGAAATTGACTTGACATTGGCCACCGGAGATTTTGAAGTGTTTGAAGCAACAAACGAAGCCATGGGTGAGGTTATGAAGGTGCTTCAGGATGATGACGTCGGTGCCGTTGTGGTCTACGGAATGGGAGGCGTCGGCAAGACAACCCTGGTAAAATATGTCGGTGCAGATGCCCGACAAAGTGGGATTTTTCATTATGCGATTATGGCTGTTGTATCCCAAAGCCCAGACATAGAAAAGATTCAAGGCACATTGGCAGATATGTTGGGCGTTAAATTGGAGGGGGAGACAGAATTTGGAAGAGCTGTTAGATTGAAAAGAGAGATAATGAGAAGAGAAAAAATCCTTATAATGTTGGACGATGTCTGGCAGAGAATAGAGTTGTCAAATATAGGGATTCCCAGCTATGAGGAGCTTCAAAAGTTCGGGTCCAAAGTGCTATTCACCACAAGGAAATTGAACGTCTGTCATGCCATGAGTTGCCAAGAAAAGATCATCACCCTCAACGTTCTCTCAGAAAAAGATTCTTGGACCTTGTTTTTAAGAAACGCAGGAACAATTGAGTCCACCGCATTTGAGGAAGTGGCGAGGAGGGTAGCTAAAGAATGCAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCAAAGGTGAGGAGGACCTGAAGGAATGGGAAAAAGCATCTCAACAACTAGAGAAGTCGCAATATGCCAATCCCAACCATGAGGAAGATGACAAAAATGCATTCAAATGTATACGATTAAGCTATGATTTCTTGAAAAATAAAGACCACAAGTCGTGCTTCTTGCTCTGTTGCATGTTCCCAGAAGACCATGAAATCAAAATAGAAGACTTGTTCCGGTACGCGATGGGAAAAAGACTGTTTCCAGAAGCCGAAACAATGGAAGTAGCCAGAGGAAGATTAGTGACAGTGGTCAAGTACCTGAAAGGTTCTAGCTTGCTTTTGGATAGCAAGAAAAAAGGATGTGTAAAGATGCATGATGTCATACGCGATACAGCCCTTCAAATTGCCGAATCTGAAGATGGCCATCAGTTTTTTGTGAAAGCTGGCTGTGGTTTAAAGGGTTGGCCGCCACATGGATTACAAGAATACTGCATTGCGATTTCACTGATGAACAACAAAATTTGCAAGCTACCGGAAGATGAGTTAGTATTTCCAAATCTCCATATTTTATTACTAAACGGGAATGCTGAACTAAGTGAGATCCCAGAAAAGTTGATCCAGAATCTGAAGGAGTTAAGGGTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCTGAATCATTTAGTGTCCTCACCAACCTTCAAGTTTTGAATTTAGATGATTGCGACAAATTGATTGACATTTCCATAGTGGGAAAACTTAAGAAGCTTGAAATTCTTAGTATGAGAGACTGTCCCGGGGGGAAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAATCGCTCTTGCCCACCAACAGTTGGAGGAATGGTTACAATTCCATCTAGAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTTAAAGACCGGCGTACGTGGACATGGCGTACATGGTTTACATGGTGTGTTTTTGGTATTTTTCCTAAGCGACCAACCAATATTATTAGGTTTGATAAGTTAGCTGGTTTATCAAATCTGAAAATATTGCAGGTTGGCTTATCTGATGAAAGTTTCATCCCTAAAAATGTTGAGGCTGCACCAGATTGGGATTACTTTTGTATAAGTATCATTGGCAGATGCACCAATACTATGTACGCCTCCGCTGCATACGAACAAGGAGATCATAATTCAAGATCCTTGATTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCAATGCAGTCATAAAGAAAACAAAGAAGCTAAAGTATGATCGCTGCGGAGGAATGAGTGACATTGTTATGGAATATGACCATGGGAGGCTACATAAACTCAAGCATCTCATAGTTTCTGGGTATTTTCTTGATTCCTATGTGTACTTGACAGAGTTGATGAACACAACAACACGAGTTGAAACAGGACCAATGTTTGAGAGTTTGGAAGAGTTGTATCTGCTAGATCTGATCCACCTTGAGGAGTTGTGTGTTGGTGAGCTACCACCTGGTTCTCTCTCAAATCTCAAGGTATTGCATATGCATGGTTGTTTTATCTTGAAGAGTGTTTCAAAATTTGTACAGAGACTACCAAATCTGGAGAAACTATATTTAGATTGGATGAAGGAATTGGAATATGTGTTTGGATCCGAAGGGTGTGAGCCAGAACAATCAAAACTGAGAGAGATGCATTTGTTGTGTCTAAATGCACTAAAAAACATATGTAATGGTCCCGCTCCACGCGCAATGTTCCAGAGTCTTAAAAGCTTGACCGTGTTCAAGTGTAACTCGTTAAAAAGTCTGTTCGCATCTGATGTAGCTCAGTATCTTGTTCAATTGGAAGACTTTCTTGTAGAGAGGTGTCCTTTGTTGGAAAGAGTAATGGATGCAGTGAACAAAGATAAGACGGTTCTACCAAAATTGAAGAACTTGGTTTTGAAGAATCTTCCTAAGTTGTATGGTGCAAGTGCTACTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGGTGGTGGAGAATTGCCCCAAGCTTCTATTTTCAACCTCTGCTACTCACTACTTTGGCAGCAGGAACTCAGTCCAACTCAATGATCCGCGACTGTACCGTTTTCTACGTGACAGATGCAAGAATGTATCTACATTTAATGAAGTCTAA

Protein Analysis

1132

Amino Acids

128.22

Weight (kDa)

6.55

Isoelectric Point (pI)

44.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 20 - 185 1.5e-51 TIR domain
TIR_2 PF13676 23 - 119 6.3e-17 TIR domain
NB-ARC PF00931 208 - 372 7.5e-32 NB-ARC domain
LRR_8 PF13855 583 - 641 1.2e-09 Leucine rich repeat
LRR_14 PF23598 590 - 760 2.5e-17 Leucine-rich repeat region
LRR_RPS2 PF23247 895 - 1034 7.8e-20 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 152, 504, 914
AatII GACGTC 1 cut(s) 666
Acc36I ACCTGC 1 cut(s) 2275
AccB1I GGYRCC 2 cut(s) 369, 668
AccB7I CCANNNNNTGG 1 cut(s) 2075
AccBSI CCGCTC 2 cut(s) 127, 2975
AccI GTMKAC 3 cut(s) 681, 1180, 1324
AccII CGCG 4 cut(s) 1436, 1581, 2982, 3337
AciI CCGC 9 cut(s) 45, 127, 335, 1134, 1661, 2397, 2526, 2973, 3335
AclI AACGTT 1 cut(s) 1071
AcoI YGGCCR 4 cut(s) 471, 585, 1615, 1658
AcsI RAATTY 6 cut(s) 327, 857, 1713, 1909, 1971, 2813
AcuI CTGAAG 4 cut(s) 635, 1253, 1629, 1841
AcyI GRCGYC 2 cut(s) 663, 696
AdeI CACNNNGTG 2 cut(s) 486, 3250
AfiI CCNNNNNNNGG 4 cut(s) 1667, 2037, 2075, 2719
AflII CTTAAG 1 cut(s) 1958
AhdI GACNNNNNGTC 1 cut(s) 1363
AhlI ACTAGT 1 cut(s) 1853
AjnI CCWGG 3 cut(s) 181, 711, 2746
AjuI GAANNNNNNNTTGG 4 cut(s) 989, 1021, 2865, 2897
Alw26I GTCTC 3 cut(s) 845, 1980, 2818
AlwNI CAGNNNCTG 1 cut(s) 1819
Ama87I CYCGRG 1 cut(s) 1994
AoxI GGCC 5 cut(s) 3, 471, 585, 1615, 1658
ApeKI GCWGC 5 cut(s) 130, 2330, 2399, 2523, 3306
ApoI RAATTY 6 cut(s) 327, 857, 1713, 1909, 1971, 2813
Asp700I GAANNNNTTC 2 cut(s) 804, 3084
AspLEI GCGC 1 cut(s) 2984
AspS9I GGNCC 5 cut(s) 993, 1095, 1228, 2666, 2969
AsuC2I CCSGG 2 cut(s) 1995, 1996
AsuHPI GGTGA 7 cut(s) 30, 644, 1000, 1054, 1232, 1882, 2747
AvaI CYCGRG 1 cut(s) 1994
AvaII GGWCC 5 cut(s) 993, 1095, 1228, 2666, 2969
BaeGI GKGCMC 1 cut(s) 1213
BalI TGGCCA 3 cut(s) 473, 587, 1617
BamHI GGATCC 2 cut(s) 187, 2885
BanI GGYRCC 2 cut(s) 369, 668
BauI CACGAG 1 cut(s) 2652
BbsI GAAGAC 3 cut(s) 1404, 1425, 3087
BbvI GCAGC 5 cut(s) 117, 2317, 2386, 2510, 3318
BccI CCATC 5 cut(s) 1432, 1607, 1626, 2104, 2462
BceAI ACGGC 2 cut(s) 383, 656
BciT130I CCWGG 3 cut(s) 183, 713, 2748
BciVI GTATCC 1 cut(s) 790
BcnI CCSGG 2 cut(s) 1995, 1996
BcoDI GTCTC 3 cut(s) 845, 1980, 2818
BcuI ACTAGT 1 cut(s) 1853
BfaI CTAG 5 cut(s) 1262, 1524, 1854, 2100, 2702
BfrI CTTAAG 1 cut(s) 1958
BfuAI ACCTGC 1 cut(s) 2275
BfuI GTATCC 1 cut(s) 790
BglII AGATCT 3 cut(s) 172, 297, 2704
BisI GCNGC 8 cut(s) 128, 131, 335, 1661, 2331, 2400, 2524, 3307
BlsI GCNGC 8 cut(s) 129, 132, 336, 1662, 2332, 2401, 2525, 3308
Bme1390I CCNGG 5 cut(s) 183, 713, 1995, 1996, 2748
Bme18I GGWCC 5 cut(s) 993, 1095, 1228, 2666, 2969
BmeRI GACNNNNNGTC 1 cut(s) 1363
BmeT110I CYCGRG 1 cut(s) 1994
BmgT120I GGNCC 5 cut(s) 993, 1095, 1228, 2666, 2969
BmiI GGNNCC 7 cut(s) 189, 354, 371, 670, 994, 2887, 2971
BmrFI CCNGG 5 cut(s) 183, 713, 1995, 1996, 2748
BmrI ACTGGG 1 cut(s) 492
BmuI ACTGGG 1 cut(s) 492
BoxI GACNNNNGTC 1 cut(s) 1502
BpiI GAAGAC 3 cut(s) 1404, 1425, 3087
BpmI CTGGAG 2 cut(s) 461, 2858
Bpu10I CCTNAGC 1 cut(s) 2220
BpuEI CTTGAG 3 cut(s) 2564, 2738, 2750
BpuMI CCSGG 2 cut(s) 1995, 1996
BsaAI YACGTR 2 cut(s) 2172, 3356
BsaBI GATNNNNATC 3 cut(s) 171, 1617, 2052
BsaHI GRCGYC 2 cut(s) 663, 696
BsaJI CCNNGG 6 cut(s) 182, 627, 711, 1994, 1995, 2560
BsaWI WCCGGW 4 cut(s) 372, 590, 1428, 1726
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bsc4I CCNNNNNNNGG 4 cut(s) 1667, 2037, 2075, 2719
Bse118I RCCGGY 1 cut(s) 2163
Bse1I ACTGG 2 cut(s) 474, 487
Bse3DI GCAATG 3 cut(s) 452, 1686, 2991
Bse8I GATNNNNATC 3 cut(s) 171, 1617, 2052
BseBI CCWGG 3 cut(s) 183, 713, 2748
BseDI CCNNGG 6 cut(s) 182, 627, 711, 1994, 1995, 2560
BseGI GGATG 7 cut(s) 169, 661, 1559, 2053, 2310, 2865, 3130
BseJI GATNNNNATC 3 cut(s) 171, 1617, 2052
BseLI CCNNNNNNNGG 4 cut(s) 1667, 2037, 2075, 2719
BseMI GCAATG 3 cut(s) 452, 1686, 2991
BseMII CTCAG 4 cut(s) 1092, 2909, 3074, 3330
BseNI ACTGG 2 cut(s) 474, 487
BseRI GAGGAG 4 cut(s) 989, 1168, 1238, 2735
BseSI GKGCMC 1 cut(s) 1213
BseXI GCAGC 5 cut(s) 117, 2317, 2386, 2510, 3318
BseYI CCCAGC 1 cut(s) 965
BsgI GTGCAG 2 cut(s) 750, 2316
Bsh1236I CGCG 4 cut(s) 1436, 1581, 2982, 3337
BshFI GGCC 5 cut(s) 5, 473, 587, 1617, 1660
BshNI GGYRCC 2 cut(s) 369, 668
BsiHKCI CYCGRG 1 cut(s) 1994
BsiSI CCGG 6 cut(s) 373, 591, 1429, 1727, 1995, 2164
BsiWI CGTACG 1 cut(s) 2168
BslFI GGGAC 2 cut(s) 1977, 2955
BslI CCNNNNNNNGG 4 cut(s) 1667, 2037, 2075, 2719
BsmAI GTCTC 3 cut(s) 845, 1980, 2818
BsmFI GGGAC 2 cut(s) 1977, 2955
BsmI GAATGC 3 cut(s) 1175, 1313, 1783
BsnI GGCC 5 cut(s) 5, 473, 587, 1617, 1660
BsoBI CYCGRG 1 cut(s) 1994
Bsp1286I GDGCHC 1 cut(s) 1213
Bsp1407I TGTACA 2 cut(s) 2136, 2818
Bsp19I CCATGG 2 cut(s) 627, 2560
BspACI CCGC 9 cut(s) 45, 127, 335, 1134, 1661, 2397, 2526, 2973, 3335
BspANI GGCC 5 cut(s) 5, 473, 587, 1617, 1660
BspCNI CTCAG 4 cut(s) 1091, 2910, 3073, 3329
BspFNI CGCG 4 cut(s) 1436, 1581, 2982, 3337
BspLI GGNNCC 7 cut(s) 189, 354, 371, 670, 994, 2887, 2971
BspMI ACCTGC 1 cut(s) 2275
BspQI GCTCTTC 1 cut(s) 859
BspT107I GGYRCC 2 cut(s) 369, 668
BspTI CTTAAG 1 cut(s) 1958
BsrBI CCGCTC 2 cut(s) 127, 2975
BsrDI GCAATG 3 cut(s) 452, 1686, 2991
BsrFI RCCGGY 1 cut(s) 2163
BsrGI TGTACA 2 cut(s) 2136, 2818
BsrI ACTGG 2 cut(s) 474, 487
BssAI RCCGGY 1 cut(s) 2163
BssECI CCNNGG 6 cut(s) 182, 627, 711, 1994, 1995, 2560
BssNAI GTATAC 1 cut(s) 1325
BssNI GRCGYC 2 cut(s) 663, 696
BssSI CACGAG 1 cut(s) 2652
BssT1I CCWWGG 2 cut(s) 627, 2560
Bst1107I GTATAC 1 cut(s) 1325
Bst2BI CACGAG 1 cut(s) 2652
Bst2UI CCWGG 3 cut(s) 183, 713, 2748
Bst6I CTCTTC 6 cut(s) 24, 271, 859, 892, 2682, 2795
BstACI GRCGYC 2 cut(s) 663, 696
BstAFI CTTAAG 1 cut(s) 1958
BstAUI TGTACA 2 cut(s) 2136, 2818
BstBAI YACGTR 2 cut(s) 2172, 3356
BstC8I GCNNGC 3 cut(s) 1529, 1639, 1721
BstDSI CCRYGG 2 cut(s) 627, 2560
BstF5I GGATG 7 cut(s) 169, 661, 1559, 2053, 2310, 2865, 3130
BstFNI CGCG 4 cut(s) 1436, 1581, 2982, 3337
BstHHI GCGC 1 cut(s) 2984
BstMAI GTCTC 3 cut(s) 845, 1980, 2818
BstMWI GCNNNNNNNGC 6 cut(s) 11, 221, 770, 1196, 2399, 2981
BstNI CCWGG 3 cut(s) 183, 713, 2748
BstNSI RCATGY 1 cut(s) 1390
BstPAI GACNNNNGTC 1 cut(s) 1502
BstSCI CCNGG 5 cut(s) 181, 711, 1993, 1994, 2746
BstSLI GKGCMC 1 cut(s) 1213
BstUI CGCG 4 cut(s) 1436, 1581, 2982, 3337
BstV1I GCAGC 5 cut(s) 117, 2317, 2386, 2510, 3318
BstV2I GAAGAC 3 cut(s) 1404, 1425, 3087
BstX2I RGATCY 8 cut(s) 172, 187, 297, 1794, 1840, 2429, 2704, 2885
BstYI RGATCY 8 cut(s) 172, 187, 297, 1794, 1840, 2429, 2704, 2885
BstZ17I GTATAC 1 cut(s) 1325
BsuI GTATCC 1 cut(s) 790
BsuRI GGCC 5 cut(s) 5, 473, 587, 1617, 1660
BtgI CCRYGG 2 cut(s) 627, 2560
BtgZI GCGATG 1 cut(s) 1451
BtsCI GGATG 7 cut(s) 169, 661, 1559, 2053, 2310, 2865, 3130
BtsI GCAGTG 1 cut(s) 3135
BtsIMutI CAGTG 5 cut(s) 88, 480, 1506, 1697, 3135
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 3 cut(s) 1529, 1639, 1721
CaiI CAGNNNCTG 1 cut(s) 1819
CfoI GCGC 1 cut(s) 2984
Cfr10I RCCGGY 1 cut(s) 2163
Cfr13I GGNCC 5 cut(s) 993, 1095, 1228, 2666, 2969
Cfr9I CCCGGG 1 cut(s) 1994
CseI GACGC 1 cut(s) 685
CsiI ACCWGGT 1 cut(s) 2746
CspCI CAANNNNNGTGG 2 cut(s) 1120, 1155
DraI TTTAAA 3 cut(s) 462, 1650, 2158
DraIII CACNNNGTG 2 cut(s) 486, 3250
DriI GACNNNNNGTC 1 cut(s) 1363
EaeI YGGCCR 4 cut(s) 471, 585, 1615, 1658
Eam1104I CTCTTC 6 cut(s) 24, 271, 859, 892, 2682, 2795
Eam1105I GACNNNNNGTC 1 cut(s) 1363
EarI CTCTTC 6 cut(s) 24, 271, 859, 892, 2682, 2795
Eco130I CCWWGG 2 cut(s) 627, 2560
Eco32I GATATC 1 cut(s) 2109
Eco47I GGWCC 5 cut(s) 993, 1095, 1228, 2666, 2969
Eco57I CTGAAG 4 cut(s) 635, 1253, 1629, 1841
Eco88I CYCGRG 1 cut(s) 1994
EcoO109I RGGNCCY 1 cut(s) 1228
EcoRI GAATTC 1 cut(s) 327
EcoRII CCWGG 3 cut(s) 181, 711, 2746
EcoRV GATATC 1 cut(s) 2109
EcoT14I CCWWGG 2 cut(s) 627, 2560
EcoT22I ATGCAT 4 cut(s) 1315, 1569, 2784, 2931
ErhI CCWWGG 2 cut(s) 627, 2560
FalI AAGNNNNNCTT 4 cut(s) 1355, 1387, 3072, 3104
FaqI GGGAC 2 cut(s) 1977, 2955
FauI CCCGC 1 cut(s) 2980
FauNDI CATATG 2 cut(s) 2778, 2960
FblI GTMKAC 3 cut(s) 681, 1180, 1324
Fnu4HI GCNGC 8 cut(s) 128, 131, 335, 1661, 2331, 2400, 2524, 3307
FokI GGATG 7 cut(s) 176, 668, 1566, 2060, 2297, 2872, 3137
Fsp4HI GCNGC 8 cut(s) 128, 131, 335, 1661, 2331, 2400, 2524, 3307
FspBI CTAG 5 cut(s) 1262, 1524, 1854, 2100, 2702
GlaI GCGC 1 cut(s) 2983
GluI GCNGC 8 cut(s) 128, 131, 335, 1661, 2331, 2400, 2524, 3307
GsaI CCCAGC 1 cut(s) 969
GsuI CTGGAG 2 cut(s) 461, 2858
HaeIII GGCC 5 cut(s) 5, 473, 587, 1617, 1660
HapII CCGG 6 cut(s) 373, 591, 1429, 1727, 1995, 2164
HgaI GACGC 1 cut(s) 685
HhaI GCGC 1 cut(s) 2984
Hin1I GRCGYC 2 cut(s) 663, 696
Hin6I GCGC 1 cut(s) 2982
HinP1I GCGC 1 cut(s) 2982
HindIII AAGCTT 4 cut(s) 393, 1964, 3004, 3269
HpaII CCGG 6 cut(s) 373, 591, 1429, 1727, 1995, 2164
HphI GGTGA 7 cut(s) 30, 644, 1000, 1054, 1232, 1882, 2747
Hpy166II GTNNAC 9 cut(s) 682, 1131, 1181, 1325, 2146, 2175, 2193, 2623, 3133
Hpy8I GTNNAC 9 cut(s) 682, 1131, 1181, 1325, 2146, 2175, 2193, 2623, 3133
Hpy99I CGWCG 2 cut(s) 668, 701
HpyCH4IV ACGT 5 cut(s) 663, 1026, 1071, 2171, 3355
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 221, 770, 1196, 2399, 2981
HpySE526I ACGT 5 cut(s) 663, 1026, 1071, 2171, 3355
Hsp92I GRCGYC 2 cut(s) 663, 696
HspAI GCGC 1 cut(s) 2982
LguI GCTCTTC 1 cut(s) 859
LmnI GCTCC 4 cut(s) 442, 976, 2447, 2980
Lsp1109I GCAGC 5 cut(s) 117, 2317, 2386, 2510, 3318
MabI ACCWGGT 1 cut(s) 2746
MaeI CTAG 5 cut(s) 1262, 1524, 1854, 2100, 2702
MaeII ACGT 5 cut(s) 663, 1026, 1071, 2171, 3355
MaeIII GTNAC 5 cut(s) 1495, 2086, 2537, 3023, 3356
MbiI CCGCTC 2 cut(s) 127, 2975
MfeI CAATTG 3 cut(s) 237, 1122, 3074
MflI RGATCY 8 cut(s) 172, 187, 297, 1794, 1840, 2429, 2704, 2885
MhlI GDGCHC 1 cut(s) 1213
MlsI TGGCCA 3 cut(s) 473, 587, 1617
MluNI TGGCCA 3 cut(s) 473, 587, 1617
MlyI GAGTC 3 cut(s) 460, 1136, 3004
MmeI TCCRAC 4 cut(s) 900, 2056, 3090, 3346
Mox20I TGGCCA 3 cut(s) 473, 587, 1617
Mph1103I ATGCAT 4 cut(s) 1315, 1569, 2784, 2931
MroXI GAANNNNTTC 2 cut(s) 804, 3084
MscI TGGCCA 3 cut(s) 473, 587, 1617
MslI CAYNNNNRTG 3 cut(s) 1318, 2546, 2781
Msp20I TGGCCA 3 cut(s) 473, 587, 1617
MspA1I CMGCKG 2 cut(s) 439, 2399
MspCI CTTAAG 1 cut(s) 1958
MspI CCGG 6 cut(s) 373, 591, 1429, 1727, 1995, 2164
MspR9I CCNGG 5 cut(s) 183, 713, 1995, 1996, 2748
MunI CAATTG 3 cut(s) 237, 1122, 3074
Mva1269I GAATGC 3 cut(s) 1175, 1313, 1783
MvaI CCWGG 3 cut(s) 183, 713, 2748
MvnI CGCG 4 cut(s) 1436, 1581, 2982, 3337
MwoI GCNNNNNNNGC 6 cut(s) 11, 221, 770, 1196, 2399, 2981
NciI CCSGG 2 cut(s) 1995, 1996
NcoI CCATGG 2 cut(s) 627, 2560
NdeI CATATG 2 cut(s) 2778, 2960
NlaIV GGNNCC 7 cut(s) 189, 354, 371, 670, 994, 2887, 2971
NmuCI GTSAC 3 cut(s) 1495, 2537, 3356
NsiI ATGCAT 4 cut(s) 1315, 1569, 2784, 2931
NspI RCATGY 1 cut(s) 1390
PciSI GCTCTTC 1 cut(s) 859
PcsI WCGNNNNNNNCGW 1 cut(s) 669
PctI GAATGC 3 cut(s) 1175, 1313, 1783
PdmI GAANNNNTTC 2 cut(s) 804, 3084
Pfl23II CGTACG 1 cut(s) 2168
PflFI GACNNNGTC 1 cut(s) 926
PflMI CCANNNNNTGG 1 cut(s) 2075
PkrI GCNGC 8 cut(s) 129, 132, 336, 1662, 2332, 2401, 2525, 3308
PleI GAGTC 3 cut(s) 459, 1135, 3003
PpsI GAGTC 3 cut(s) 459, 1135, 3003
Ppu21I YACGTR 2 cut(s) 2172, 3356
PpuMI RGGWCCY 1 cut(s) 1228
PshAI GACNNNNGTC 1 cut(s) 1502
PsiI TTATAA 3 cut(s) 152, 504, 914
Psp1406I AACGTT 1 cut(s) 1071
Psp5II RGGWCCY 1 cut(s) 1228
Psp6I CCWGG 3 cut(s) 181, 711, 2746
PspFI CCCAGC 1 cut(s) 965
PspGI CCWGG 3 cut(s) 181, 711, 2746
PspLI CGTACG 1 cut(s) 2168
PspN4I GGNNCC 7 cut(s) 189, 354, 371, 670, 994, 2887, 2971
PspPI GGNCC 5 cut(s) 993, 1095, 1228, 2666, 2969
PspPPI RGGWCCY 1 cut(s) 1228
PstNI CAGNNNCTG 1 cut(s) 1819
PsuI RGATCY 8 cut(s) 172, 187, 297, 1794, 1840, 2429, 2704, 2885
PsyI GACNNNGTC 1 cut(s) 926
PvuII CAGCTG 1 cut(s) 439
RseI CAYNNNNRTG 3 cut(s) 1318, 2546, 2781
SapI GCTCTTC 1 cut(s) 859
SatI GCNGC 8 cut(s) 128, 131, 335, 1661, 2331, 2400, 2524, 3307
Sau96I GGNCC 5 cut(s) 993, 1095, 1228, 2666, 2969
SchI GAGTC 3 cut(s) 460, 1136, 3004
ScrFI CCNGG 5 cut(s) 183, 713, 1995, 1996, 2748
SduI GDGCHC 1 cut(s) 1213
SexAI ACCWGGT 1 cut(s) 2746
SinI GGWCC 5 cut(s) 993, 1095, 1228, 2666, 2969
SmaI CCCGGG 1 cut(s) 1996
SmiMI CAYNNNNRTG 3 cut(s) 1318, 2546, 2781
SmlI CTYRAG 4 cut(s) 1958, 2579, 2717, 2765
SmoI CTYRAG 4 cut(s) 1958, 2579, 2717, 2765
SpeI ACTAGT 1 cut(s) 1853
SsiI CCGC 9 cut(s) 45, 127, 335, 1134, 1661, 2397, 2526, 2973, 3335
SspI AATATT 2 cut(s) 2236, 2280
SspMI CTAG 5 cut(s) 1262, 1524, 1854, 2100, 2702
StyD4I CCNGG 5 cut(s) 181, 711, 1993, 1994, 2746
StyI CCWWGG 2 cut(s) 627, 2560
TaiI ACGT 5 cut(s) 666, 1029, 1074, 2174, 3358
TaqI TCGA 4 cut(s) 97, 169, 399, 2009
TatI WGTACW 4 cut(s) 2136, 2145, 2622, 2818
TauI GCSGC 3 cut(s) 130, 337, 1663
TscAI CASTG 5 cut(s) 88, 487, 1506, 1704, 3135
TseFI GTSAC 3 cut(s) 1495, 2537, 3356
TseI GCWGC 5 cut(s) 130, 2330, 2399, 2523, 3306
Tsp45I GTSAC 3 cut(s) 1495, 2537, 3356
TspGWI ACGGA 3 cut(s) 42, 699, 1852
TspMI CCCGGG 1 cut(s) 1994
TspRI CASTG 5 cut(s) 88, 487, 1506, 1704, 3135
Tth111I GACNNNGTC 1 cut(s) 926
Van91I CCANNNNNTGG 1 cut(s) 2075
Vha464I CTTAAG 1 cut(s) 1958
VpaK11BI GGWCC 5 cut(s) 993, 1095, 1228, 2666, 2969
XapI RAATTY 6 cut(s) 327, 857, 1713, 1909, 1971, 2813
XbaI TCTAGA 1 cut(s) 2099
XceI RCATGY 1 cut(s) 1390
XmaI CCCGGG 1 cut(s) 1994
XmiI GTMKAC 3 cut(s) 681, 1180, 1324
XmnI GAANNNNTTC 2 cut(s) 804, 3084
XspI CTAG 5 cut(s) 1262, 1524, 1854, 2100, 2702
ZraI GACGTC 1 cut(s) 664
Zsp2I ATGCAT 4 cut(s) 1315, 1569, 2784, 2931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.