RchiOBHm_Chr5g0079821

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
85584032 .. 85592902
8871 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35419

Sequence Viewer

Length: 3033 bp
ATGGATGATCGAGATCTTGAAGTAGGGGATGTTATTTCTCCAACTCTCTTAAGAGCAATTAGAGAATCAAGGTTTGCAATCGTTATTCTCTCGCAAAATTATGCTTCTTCTACTTGGTGTTTGGAGGAACTTAGAGAGATTTGTCTATGCATGGAAGACAGCAGTAGAATCCTGCCACTCTTTTATCAGGTTGATCCTACTGATGTTCGATATCAGAAGAGGAGTTTTGAAGAAGCTTTCACTAGGCATGAAACCTCTGGGCGACATGAATCAGAGAAGGTGGAAAAGTGGAAAGCTGCTCTAAACAAAGTGGCCAATATCTCTGGGTGGAATACAAATGATCATAAGACTCACCAAGAACTGATCAACTCCATTGTGGAATCTGTCTCCTGTAAAGTCTTACCTGCTGCAGTCGAGCCCATGGGAGAGTTCAAAGAGTTTCAAGCAACGAGACAAGCCATGAATGAAGTTATGCAGGCGCTAAAAGATGATGAAGTCAATGCCGTTGGCGTGTACGGCATGGGAGGCGTCGGCAAGACAACCATGGTAAAACATGTCGGTGCACAAGCCTGCAAAAATGGGATTTTTGATCGTGTGATTATGGCTGTCGTTTCACAAAATCCTAATTTCGAGAAAATTCAAGGCACATTGGCAGAGCAGTTGGGCTTCAAATTGTGGGAGGAGACAGAAATTGGACGAATTGCTAGATTGAATAAGGAAATAATGAGAAGAGAAAAACTCCTTATAATCTTGGACGATGTTTGGGGGAGAACAGAGTTATCAAATATAGGGATTCCCATCTACAAGGAACTTCAAAAGTGCAAGTCCAAAGTCCTACTCACCACAAGGATAAGGAATGTCTGTCATGCCATGGAGTGCCAAAAAAGGGTCACCCTCACTGTCCTCTCAAAAGAAGATTCTTGGACTTTGTTTGTGACAAAAGCAAGAAGGTCTTTTGAGTCCACCAAATTTGAGGATGTGGCAAGGAGGGTAGCTGGAGAATGTAGGGGTCTACCGATAGCATTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGACTTAGCGGAATGGGAAAAAGCAGCTCAACGACTAGAGAAGTCGCAAATTGCCAACCCTGACCATGATGAAGATGCATTCAAATGTATAAGATTAAGCTATGATTACTTGAAACATGAAGACCACAAGTCATGCTTCCTGCTCTGTTGCCTATACCCAGAAGACCATGACATTCGACTAGAAAACTTGTTCAAGTATGCAATCGGGAAAGGATTGTTTCGTGATGCCGAAACAATCGAAGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGCGAGAAAAAAGGATGTGTAAAGATGCATGATGTCATCCGGGATACTGCCCTGAATATTGCAAAATCTAAAGATGGGCACGGGTTTCTGGTAAAAGCGGGCTGTGGTATAAAGGATTGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAGAATTCGCAAGCTACCCGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAAACGGAATGATGATTTGAACAATATCCCAGAAAAGTTTGTCCAAAGTCTGAATGAATTAAGGGTCTTGGATCTTAGCATCACTAGTATTTCCTTACTACCCCAATCATTCAGTCTCCTTACCAATCTCCAAGCTTTGTATTTAGATTTTTGCTGGAAATTGACTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGAGAAAATCGTCTTAGGAAATTGTCAAGAGAAATAGGAAATTTGACCAATCTAAGGATGTTGGATATCCGTGCCGACAAACCAGTAGCTGGAGGTGTTATCACAATTCCTGTTGGAGTTCCGAATTGGGTTTACTTTGATATACGTATCAACGAAGGCTCATGGGATCCATACGATCAACAAGATCATAATTCAAGAACCTTGGTTCTTCGTTTCACAAGCATCAGTACCTTACCAGATTGGTTTGTGAATGCGGTGACAACGAAAACAGAGAAGCTAGCGTATGACTGCTACCGAGGGATGAGTGACATTCTTATGGAATATGACCACGGGAGGTTGCATGAACTAAAGCATCTCATTGTATCTGGTTCTCTTTTTGGTTCCTGTAAGTACTTAGAAGAGTTGATGAACACAAGAAGACGGGTTCAAAAAGGGCCAGTATTTGAGAACTTAGAAGAGTTGCATCTGATACATCTGATCCACCTTAATGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCATTAATCTGAAGGTGTTTCATGTGTTTGGTTGTATGAAATTGGGGAACGTATCAAAGTTGGTACAGAAACTACCAAATCTGGAGAAACTTTATTTAAACTATATGCGTGGATTGGAATATGTGTTTGGATGTGAAGTGGATCAGCCAGCACAATCAAAACTGAGAGAGATATATTTGTTGGGTCCAAATTCAGTGAAAAGCATTTGTAATGGTCCCGTCCCACGTGTAATGTTCCAGAGTCTTAAAAGTTTGACCTTTTACCGTTGTGAGGTGCTGCAAAGTCTCTTCGCATCTGATGTAGCTCAGTGTCTTGTTCAATTGGAAGACCTTTTTGTACAACAGTGTCCTTTGTTGGAAAGGGTAATTGAAGCTGTGAACGACGAGAGACCGGTTCTTCCAAGATTGAAGAACTTGGTTTTGATGCAACTTCCTATGTTGTATGGTGCAAGTGCTACAGGTGAAATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGCCCCCAATTTTCATTTTCAATCTCTTCCTCTCTCTTCTTCACCTTCAGAAGCAAGAACCGGTTTTCATTTTCAACTTCTGCTTCTTGCTACTTTGGCAGCAGGAACATAATCAAACTCAATGATGAACAACTGTACAGCTTTCTACGTTACAGATGGGGAGCACGTAATGAATCCGCTGACTTGCAATGGTCCCTCATCAGCTTCTGA

Protein Analysis

1010

Amino Acids

115.31

Weight (kDa)

7.24

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR_2 PF13676 1 - 66 4.9e-08 TIR domain
TIR PF01582 2 - 134 2.2e-35 TIR domain
NB-ARC PF00931 152 - 315 3.3e-33 NB-ARC domain
nSTAND3 PF20720 152 - 295 6.4e-06 Novel STAND NTPase 3
LRR_8 PF13855 521 - 578 4.6e-07 Leucine rich repeat
LRR_14 PF23598 527 - 626 1.3e-11 Leucine-rich repeat region
LRR_RPS2 PF23247 750 - 889 2.6e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 835 - 950 4.5e-13 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 746
Acc36I ACCTGC 1 cut(s) 412
AccB7I CCANNNNNTGG 1 cut(s) 1886
AccI GTMKAC 1 cut(s) 1012
AccII CGCG 1 cut(s) 1485
AciI CCGC 5 cut(s) 1064, 1457, 1485, 2051, 3000
AclWI GGATC 6 cut(s) 188, 1662, 1958, 1971, 2269, 2469
AcoI YGGCCR 2 cut(s) 312, 1478
AcsI RAATTY 6 cut(s) 636, 968, 1530, 1785, 1837, 2509
AcuI CTGAAG 3 cut(s) 1793, 2351, 2854
AcvI CACGTG 1 cut(s) 2546
AcyI GRCGYC 1 cut(s) 528
AfaI GTAC 7 cut(s) 515, 1331, 2026, 2189, 2385, 2658, 2960
AfiI CCNNNNNNNGG 4 cut(s) 886, 1851, 1886, 2590
AflII CTTAAG 1 cut(s) 49
AflIII ACRYGT 2 cut(s) 553, 2545
AgeI ACCGGT 2 cut(s) 2710, 2883
AhdI GACNNNNNGTC 1 cut(s) 1183
AhlI ACTAGT 1 cut(s) 1667
AjnI CCWGG 1 cut(s) 2311
AjuI GAANNNNNNNTTGG 2 cut(s) 2430, 2462
Alw21I GWGCWC 2 cut(s) 565, 2989
Alw26I GTCTC 7 cut(s) 391, 445, 677, 1703, 2322, 2609, 2701
Alw44I GTGCAC 1 cut(s) 561
AlwI GGATC 6 cut(s) 188, 1662, 1958, 1971, 2269, 2469
AlwNI CAGNNNCTG 2 cut(s) 1886, 3030
AoxI GGCC 3 cut(s) 312, 1478, 2231
ApaLI GTGCAC 1 cut(s) 561
ApeKI GCWGC 6 cut(s) 296, 407, 1079, 1500, 2596, 2922
ApoI RAATTY 6 cut(s) 636, 968, 1530, 1785, 1837, 2509
AseI ATTAAT 1 cut(s) 2325
AsiGI ACCGGT 2 cut(s) 2710, 2883
AspLEI GCGC 1 cut(s) 481
AspS9I GGNCC 4 cut(s) 2231, 2504, 2534, 3015
AsuC2I CCSGG 1 cut(s) 1400
AsuHPI GGTGA 7 cut(s) 344, 832, 883, 2065, 2312, 2792, 2857
AsuNHI GCTAGC 1 cut(s) 2074
AvaII GGWCC 3 cut(s) 2504, 2534, 3015
BaeGI GKGCMC 3 cut(s) 565, 1045, 1440
BalI TGGCCA 2 cut(s) 314, 1480
BamHI GGATCC 1 cut(s) 1963
BanII GRGCYC 1 cut(s) 420
BbrPI CACGTG 1 cut(s) 2546
BbsI GAAGAC 5 cut(s) 162, 1182, 1224, 2221, 2652
Bbv12I GWGCWC 2 cut(s) 565, 2989
BbvI GCAGC 6 cut(s) 283, 394, 1091, 1487, 2583, 2934
BccI CCATC 3 cut(s) 806, 1427, 2973
BceAI ACGGC 2 cut(s) 488, 532
BciT130I CCWGG 1 cut(s) 2313
BciVI GTATCC 1 cut(s) 1396
BclI TGATCA 2 cut(s) 340, 363
BcnI CCSGG 1 cut(s) 1400
BcoDI GTCTC 7 cut(s) 391, 445, 677, 1703, 2322, 2609, 2701
BcuI ACTAGT 1 cut(s) 1667
BfaI CTAG 7 cut(s) 243, 705, 1091, 1235, 1344, 1668, 2075
BfmI CTRYAG 3 cut(s) 408, 1758, 2775
BfoI RGCGCY 1 cut(s) 482
BfrI CTTAAG 1 cut(s) 49
BfuAI ACCTGC 1 cut(s) 412
BfuI GTATCC 1 cut(s) 1396
BglII AGATCT 1 cut(s) 13
BisI GCNGC 6 cut(s) 297, 408, 1080, 1501, 2597, 2923
BlsI GCNGC 6 cut(s) 298, 409, 1081, 1502, 2598, 2924
BmcAI AGTACT 1 cut(s) 2189
Bme1390I CCNGG 2 cut(s) 1400, 2313
Bme18I GGWCC 3 cut(s) 2504, 2534, 3015
BmeRI GACNNNNNGTC 1 cut(s) 1183
BmgT120I GGNCC 4 cut(s) 2231, 2504, 2534, 3015
BmiI GGNNCC 5 cut(s) 1965, 2179, 2505, 2536, 3017
BmrFI CCNGG 2 cut(s) 1400, 2313
BmsI GCATC 9 cut(s) 1120, 1270, 1374, 1671, 2028, 2158, 2269, 2621, 2733
BmtI GCTAGC 1 cut(s) 2078
BpiI GAAGAC 5 cut(s) 162, 1182, 1224, 2221, 2652
BplI GAGNNNNNCTC 2 cut(s) 723, 755
BpmI CTGGAG 4 cut(s) 1017, 1553, 1908, 2423
BpuMI CCSGG 1 cut(s) 1400
BsaAI YACGTR 3 cut(s) 1943, 2546, 2990
BsaBI GATNNNNATC 2 cut(s) 12, 797
BsaHI GRCGYC 1 cut(s) 528
BsaI GGTCTC 2 cut(s) 2322, 2701
BsaJI CCNNGG 7 cut(s) 420, 543, 870, 1998, 2092, 2125, 2312
BsaWI WCCGGW 2 cut(s) 2710, 2883
Bsc4I CCNNNNNNNGG 4 cut(s) 886, 1851, 1886, 2590
Bse118I RCCGGY 2 cut(s) 2710, 2883
Bse1I ACTGG 2 cut(s) 1880, 2234
Bse3DI GCAATG 1 cut(s) 3017
Bse8I GATNNNNATC 2 cut(s) 12, 797
BseBI CCWGG 1 cut(s) 2313
BseDI CCNNGG 7 cut(s) 420, 543, 870, 1998, 2092, 2125, 2312
BseGI GGATG 8 cut(s) 10, 34, 982, 1379, 1395, 1860, 2103, 2456
BseJI GATNNNNATC 2 cut(s) 12, 797
BseLI CCNNNNNNNGG 4 cut(s) 886, 1851, 1886, 2590
BseMI GCAATG 1 cut(s) 3017
BseMII CTCAG 2 cut(s) 2474, 2639
BseNI ACTGG 2 cut(s) 1880, 2234
BseRI GAGGAG 2 cut(s) 235, 695
BseSI GKGCMC 3 cut(s) 565, 1045, 1440
BseXI GCAGC 6 cut(s) 283, 394, 1091, 1487, 2583, 2934
BsgI GTGCAG 1 cut(s) 1486
Bsh1236I CGCG 1 cut(s) 1485
BshFI GGCC 3 cut(s) 314, 1480, 2233
BshTI ACCGGT 2 cut(s) 2710, 2883
BsiHKAI GWGCWC 2 cut(s) 565, 2989
BsiSI CCGG 3 cut(s) 1399, 2711, 2884
BslFI GGGAC 3 cut(s) 2520, 2525, 3001
BslI CCNNNNNNNGG 4 cut(s) 886, 1851, 1886, 2590
BsmAI GTCTC 7 cut(s) 391, 445, 677, 1703, 2322, 2609, 2701
BsmFI GGGAC 3 cut(s) 2520, 2525, 3001
BsmI GAATGC 2 cut(s) 1133, 2053
BsnI GGCC 3 cut(s) 314, 1480, 2233
Bso31I GGTCTC 2 cut(s) 2322, 2701
Bsp1286I GDGCHC 5 cut(s) 420, 565, 1045, 1440, 2989
Bsp1407I TGTACA 2 cut(s) 2656, 2958
Bsp19I CCATGG 3 cut(s) 420, 543, 870
BspACI CCGC 5 cut(s) 1064, 1457, 1485, 2051, 3000
BspANI GGCC 3 cut(s) 314, 1480, 2233
BspCNI CTCAG 2 cut(s) 2475, 2638
BspFNI CGCG 1 cut(s) 1485
BspLI GGNNCC 5 cut(s) 1965, 2179, 2505, 2536, 3017
BspMAI CTGCAG 1 cut(s) 412
BspMI ACCTGC 1 cut(s) 412
BspOI GCTAGC 1 cut(s) 2078
BspPI GGATC 6 cut(s) 188, 1662, 1958, 1971, 2269, 2469
BspTI CTTAAG 1 cut(s) 49
BspTNI GGTCTC 2 cut(s) 2322, 2701
BsrDI GCAATG 1 cut(s) 3017
BsrFI RCCGGY 2 cut(s) 2710, 2883
BsrGI TGTACA 2 cut(s) 2656, 2958
BsrI ACTGG 2 cut(s) 1880, 2234
BssAI RCCGGY 2 cut(s) 2710, 2883
BssECI CCNNGG 7 cut(s) 420, 543, 870, 1998, 2092, 2125, 2312
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 4 cut(s) 420, 543, 870, 1998
Bst2UI CCWGG 1 cut(s) 2313
Bst4CI ACNGT 4 cut(s) 901, 2585, 2664, 2958
Bst6I CTCTTC 8 cut(s) 212, 724, 1542, 2190, 2247, 2612, 2854, 2864
BstACI GRCGYC 1 cut(s) 528
BstAFI CTTAAG 1 cut(s) 49
BstAUI TGTACA 2 cut(s) 2656, 2958
BstBAI YACGTR 3 cut(s) 1943, 2546, 2990
BstC8I GCNNGC 7 cut(s) 477, 571, 1349, 1459, 1538, 2076, 2469
BstDSI CCRYGG 4 cut(s) 420, 543, 870, 2125
BstEII GGTNACC 1 cut(s) 889
BstF5I GGATG 8 cut(s) 10, 34, 982, 1379, 1395, 1860, 2103, 2456
BstFNI CGCG 1 cut(s) 1485
BstH2I RGCGCY 1 cut(s) 482
BstHHI GCGC 1 cut(s) 481
BstMAI GTCTC 7 cut(s) 391, 445, 677, 1703, 2322, 2609, 2701
BstMWI GCNNNNNNNGC 4 cut(s) 516, 525, 1028, 2919
BstNI CCWGG 1 cut(s) 2313
BstNSI RCATGY 1 cut(s) 557
BstPI GGTNACC 1 cut(s) 889
BstSCI CCNGG 2 cut(s) 1398, 2311
BstSFI CTRYAG 3 cut(s) 408, 1758, 2775
BstSLI GKGCMC 3 cut(s) 565, 1045, 1440
BstSNI TACGTA 1 cut(s) 1943
BstUI CGCG 1 cut(s) 1485
BstV1I GCAGC 6 cut(s) 283, 394, 1091, 1487, 2583, 2934
BstV2I GAAGAC 5 cut(s) 162, 1182, 1224, 2221, 2652
BstX2I RGATCY 3 cut(s) 13, 1654, 1963
BstYI RGATCY 3 cut(s) 13, 1654, 1963
BsuI GTATCC 1 cut(s) 1396
BsuRI GGCC 3 cut(s) 314, 1480, 2233
BtgI CCRYGG 4 cut(s) 420, 543, 870, 2125
BtsCI GGATG 8 cut(s) 10, 34, 982, 1379, 1395, 1860, 2103, 2456
BtsI GCAGTG 1 cut(s) 1503
BtsIMutI CAGTG 6 cut(s) 897, 1326, 1503, 2520, 2633, 2669
BveI ACCTGC 1 cut(s) 412
Cac8I GCNNGC 7 cut(s) 477, 571, 1349, 1459, 1538, 2076, 2469
CaiI CAGNNNCTG 2 cut(s) 1886, 3030
CfoI GCGC 1 cut(s) 481
Cfr10I RCCGGY 2 cut(s) 2710, 2883
Cfr13I GGNCC 4 cut(s) 2231, 2504, 2534, 3015
CseI GACGC 1 cut(s) 517
Csp6I GTAC 7 cut(s) 514, 1330, 2025, 2188, 2384, 2657, 2959
CspAI ACCGGT 2 cut(s) 2710, 2883
CspCI CAANNNNNGTGG 2 cut(s) 952, 987
CviQI GTAC 7 cut(s) 514, 1330, 2025, 2188, 2384, 2657, 2959
DraI TTTAAA 1 cut(s) 2418
DriI GACNNNNNGTC 1 cut(s) 1183
EaeI YGGCCR 2 cut(s) 312, 1478
Eam1104I CTCTTC 8 cut(s) 212, 724, 1542, 2190, 2247, 2612, 2854, 2864
Eam1105I GACNNNNNGTC 1 cut(s) 1183
EarI CTCTTC 8 cut(s) 212, 724, 1542, 2190, 2247, 2612, 2854, 2864
Eco105I TACGTA 1 cut(s) 1943
Eco130I CCWWGG 4 cut(s) 420, 543, 870, 1998
Eco24I GRGCYC 1 cut(s) 420
Eco31I GGTCTC 2 cut(s) 2322, 2701
Eco32I GATATC 2 cut(s) 212, 1864
Eco47I GGWCC 3 cut(s) 2504, 2534, 3015
Eco57I CTGAAG 3 cut(s) 1793, 2351, 2854
Eco72I CACGTG 1 cut(s) 2546
Eco91I GGTNACC 1 cut(s) 889
EcoO65I GGTNACC 1 cut(s) 889
EcoRI GAATTC 1 cut(s) 1530
EcoRII CCWGG 1 cut(s) 2311
EcoRV GATATC 2 cut(s) 212, 1864
EcoT14I CCWWGG 4 cut(s) 420, 543, 870, 1998
EcoT22I ATGCAT 3 cut(s) 152, 1135, 1389
EcoT38I GRGCYC 1 cut(s) 420
ErhI CCWWGG 4 cut(s) 420, 543, 870, 1998
FalI AAGNNNNNCTT 4 cut(s) 937, 969, 1175, 1207
FaqI GGGAC 3 cut(s) 2520, 2525, 3001
FauI CCCGC 1 cut(s) 1450
FbaI TGATCA 2 cut(s) 340, 363
FblI GTMKAC 1 cut(s) 1012
Fnu4HI GCNGC 6 cut(s) 297, 408, 1080, 1501, 2597, 2923
FokI GGATG 8 cut(s) 17, 41, 989, 1382, 1386, 1867, 2110, 2463
FriOI GRGCYC 1 cut(s) 420
Fsp4HI GCNGC 6 cut(s) 297, 408, 1080, 1501, 2597, 2923
FspBI CTAG 7 cut(s) 243, 705, 1091, 1235, 1344, 1668, 2075
GlaI GCGC 1 cut(s) 480
GluI GCNGC 6 cut(s) 297, 408, 1080, 1501, 2597, 2923
GsuI CTGGAG 4 cut(s) 1017, 1553, 1908, 2423
HaeII RGCGCY 1 cut(s) 482
HaeIII GGCC 3 cut(s) 314, 1480, 2233
HapII CCGG 3 cut(s) 1399, 2711, 2884
HgaI GACGC 1 cut(s) 517
HhaI GCGC 1 cut(s) 481
Hin1I GRCGYC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 479
HinP1I GCGC 1 cut(s) 479
HindIII AAGCTT 3 cut(s) 234, 1716, 1778
HpaII CCGG 3 cut(s) 1399, 2711, 2884
HphI GGTGA 7 cut(s) 344, 832, 883, 2065, 2312, 2792, 2857
Hpy166II GTNNAC 6 cut(s) 514, 563, 963, 1013, 1930, 2698
Hpy8I GTNNAC 6 cut(s) 514, 563, 963, 1013, 1930, 2698
Hpy99I CGWCG 2 cut(s) 533, 2705
HpyAV CCTTC 7 cut(s) 271, 942, 1490, 1946, 2326, 2805, 2878
HpyCH4III ACNGT 4 cut(s) 901, 2585, 2664, 2958
HpyCH4IV ACGT 5 cut(s) 1942, 2370, 2545, 2971, 2989
HpyF10VI GCNNNNNNNGC 4 cut(s) 516, 525, 1028, 2919
HpySE526I ACGT 5 cut(s) 1942, 2370, 2545, 2971, 2989
Hsp92I GRCGYC 1 cut(s) 528
HspAI GCGC 1 cut(s) 479
Ksp22I TGATCA 2 cut(s) 340, 363
LmnI GCTCC 1 cut(s) 2984
Lsp1109I GCAGC 6 cut(s) 283, 394, 1091, 1487, 2583, 2934
LweI GCATC 9 cut(s) 1120, 1270, 1374, 1671, 2028, 2158, 2269, 2621, 2733
MaeI CTAG 7 cut(s) 243, 705, 1091, 1235, 1344, 1668, 2075
MaeII ACGT 5 cut(s) 1942, 2370, 2545, 2971, 2989
MaeIII GTNAC 6 cut(s) 889, 934, 2053, 2102, 2304, 2972
MfeI CAATTG 1 cut(s) 2639
MflI RGATCY 3 cut(s) 13, 1654, 1963
MhlI GDGCHC 5 cut(s) 420, 565, 1045, 1440, 2989
MlsI TGGCCA 2 cut(s) 314, 1480
MluNI TGGCCA 2 cut(s) 314, 1480
MlyI GAGTC 3 cut(s) 343, 968, 2569
MmeI TCCRAC 4 cut(s) 65, 1839, 1891, 2655
Mox20I TGGCCA 2 cut(s) 314, 1480
Mph1103I ATGCAT 3 cut(s) 152, 1135, 1389
MscI TGGCCA 2 cut(s) 314, 1480
MseI TTAA 7 cut(s) 50, 1151, 1643, 2283, 2325, 2417, 2565
MslI CAYNNNNRTG 4 cut(s) 558, 1138, 2111, 2283
Msp20I TGGCCA 2 cut(s) 314, 1480
MspA1I CMGCKG 1 cut(s) 3002
MspCI CTTAAG 1 cut(s) 49
MspI CCGG 3 cut(s) 1399, 2711, 2884
MspR9I CCNGG 2 cut(s) 1400, 2313
MunI CAATTG 1 cut(s) 2639
Mva1269I GAATGC 2 cut(s) 1133, 2053
MvaI CCWGG 1 cut(s) 2313
MvnI CGCG 1 cut(s) 1485
MwoI GCNNNNNNNGC 4 cut(s) 516, 525, 1028, 2919
NciI CCSGG 1 cut(s) 1400
NcoI CCATGG 3 cut(s) 420, 543, 870
NheI GCTAGC 1 cut(s) 2074
NlaIV GGNNCC 5 cut(s) 1965, 2179, 2505, 2536, 3017
NmuCI GTSAC 4 cut(s) 889, 934, 2053, 2102
NsiI ATGCAT 3 cut(s) 152, 1135, 1389
NspI RCATGY 1 cut(s) 557
PciI ACATGT 1 cut(s) 553
PctI GAATGC 2 cut(s) 1133, 2053
PfeI GAWTC 9 cut(s) 65, 168, 269, 380, 793, 917, 1316, 1340, 2996
PflMI CCANNNNNTGG 1 cut(s) 1886
PfoI TCCNGGA 1 cut(s) 1398
PinAI ACCGGT 2 cut(s) 2710, 2883
PkrI GCNGC 6 cut(s) 298, 409, 1081, 1502, 2598, 2924
PleI GAGTC 3 cut(s) 343, 967, 2568
PmaCI CACGTG 1 cut(s) 2546
PmlI CACGTG 1 cut(s) 2546
PpsI GAGTC 3 cut(s) 343, 967, 2568
Ppu21I YACGTR 3 cut(s) 1943, 2546, 2990
PscI ACATGT 1 cut(s) 553
PshBI ATTAAT 1 cut(s) 2325
PsiI TTATAA 1 cut(s) 746
Psp6I CCWGG 1 cut(s) 2311
PspCI CACGTG 1 cut(s) 2546
PspEI GGTNACC 1 cut(s) 889
PspGI CCWGG 1 cut(s) 2311
PspN4I GGNNCC 5 cut(s) 1965, 2179, 2505, 2536, 3017
PspPI GGNCC 4 cut(s) 2231, 2504, 2534, 3015
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 2 cut(s) 1886, 3030
PsuI RGATCY 3 cut(s) 13, 1654, 1963
RsaI GTAC 7 cut(s) 515, 1331, 2026, 2189, 2385, 2658, 2960
RsaNI GTAC 7 cut(s) 514, 1330, 2025, 2188, 2384, 2657, 2959
RseI CAYNNNNRTG 4 cut(s) 558, 1138, 2111, 2283
SaqAI TTAA 7 cut(s) 50, 1151, 1643, 2283, 2325, 2417, 2565
SatI GCNGC 6 cut(s) 297, 408, 1080, 1501, 2597, 2923
Sau96I GGNCC 4 cut(s) 2231, 2504, 2534, 3015
ScaI AGTACT 1 cut(s) 2189
SchI GAGTC 3 cut(s) 343, 968, 2569
ScrFI CCNGG 2 cut(s) 1400, 2313
SduI GDGCHC 5 cut(s) 420, 565, 1045, 1440, 2989
SfaNI GCATC 9 cut(s) 1120, 1270, 1374, 1671, 2028, 2158, 2269, 2621, 2733
SfcI CTRYAG 3 cut(s) 408, 1758, 2775
SinI GGWCC 3 cut(s) 2504, 2534, 3015
SmiMI CAYNNNNRTG 4 cut(s) 558, 1138, 2111, 2283
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
SnaBI TACGTA 1 cut(s) 1943
SpeI ACTAGT 1 cut(s) 1667
SsiI CCGC 5 cut(s) 1064, 1457, 1485, 2051, 3000
SspI AATATT 1 cut(s) 1417
SspMI CTAG 7 cut(s) 243, 705, 1091, 1235, 1344, 1668, 2075
StyD4I CCNGG 2 cut(s) 1398, 2311
StyI CCWWGG 4 cut(s) 420, 543, 870, 1998
TaaI ACNGT 4 cut(s) 901, 2585, 2664, 2958
TaiI ACGT 5 cut(s) 1945, 2373, 2548, 2974, 2992
TaqI TCGA 6 cut(s) 10, 208, 414, 630, 1231, 1293
TatI WGTACW 3 cut(s) 2187, 2656, 2958
TfiI GAWTC 9 cut(s) 65, 168, 269, 380, 793, 917, 1316, 1340, 2996
Tru1I TTAA 7 cut(s) 50, 1151, 1643, 2283, 2325, 2417, 2565
Tru9I TTAA 7 cut(s) 50, 1151, 1643, 2283, 2325, 2417, 2565
TscAI CASTG 6 cut(s) 904, 1326, 1510, 2520, 2633, 2669
TseFI GTSAC 4 cut(s) 889, 934, 2053, 2102
TseI GCWGC 6 cut(s) 296, 407, 1079, 1500, 2596, 2922
Tsp45I GTSAC 4 cut(s) 889, 934, 2053, 2102
TspGWI ACGGA 2 cut(s) 1603, 1856
TspRI CASTG 6 cut(s) 904, 1326, 1510, 2520, 2633, 2669
Van91I CCANNNNNTGG 1 cut(s) 1886
Vha464I CTTAAG 1 cut(s) 49
VneI GTGCAC 1 cut(s) 561
VpaK11BI GGWCC 3 cut(s) 2504, 2534, 3015
VspI ATTAAT 1 cut(s) 2325
XapI RAATTY 6 cut(s) 636, 968, 1530, 1785, 1837, 2509
XceI RCATGY 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 1012
XspI CTAG 7 cut(s) 243, 705, 1091, 1235, 1344, 1668, 2075
ZrmI AGTACT 1 cut(s) 2189
Zsp2I ATGCAT 3 cut(s) 152, 1135, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.