FvH4_3g45643

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Forward (+)
37844058 .. 37848177
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g45643.t1

Sequence Viewer

Length: 3519 bp
ATGGATTTCTATTTCTTCGAGATATCGTTGGCGATTGTTGCGGTAATTGTGACATGGATGGGTTGCAAGGCCATTAAGGGGATATGGACTCGAGTAAAGCGTCGGATAAGTTACATGGTCAGAAAGTCGCAGGATAATCAAGGAGTGGTCAACTTGAATGCCTCTGAATCGCTTGCTTCATCATCAGAACCTCGTCGCTGGAAGTATGATGTGTTTTTGAGTTTCCGGGGTCCTGATACTCGCAAAGGGATTACAGCCCAAATACGCGATCGACTTCGTAGAAGTGGCATTATAACGTTCATGGATGAACCAGATCTTAGAGTAGGGGATACCATTTCGCCCACTCTCATAAAGGCAATTAAAGAGTCAAGGTTTGCAATCGTGATTCTCTCGCAAAATTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTCGTGAGATTTGTCAATCCATGGAAGAGAGTAGAATTTTGCCACTTTTTTTTGAAGTTGATCCTACTGATGTTCGATATCAGAAGAGGAGTTTCGAAGAAGCTTTCTCTAAGCACGATACCTCTGGGCGACATGAATCAGAGAAGGTGGAGCAATGGAGAGCTGCTCTAGAAAAAGTGGCCAATATCTCAGGGTGGAATACAAATGATTTCAAGACTCACAGTGAACTTGTTGACAAGATAGTGGAATCTCTCCGCCGTAAAGTACCACCTGATGCAATTGAATTCACAGATGATTTCCAAGCATTTGGAGCAACAAAAGAAGCGACAGACAAGGTTATGAAGGCGCTAACAGATGACAAGATTACTGCCGTTGGTGTCTATGGCATGGGTGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAGAAATGGTTTTTTTAACCATTGGATTATGGGTGTTGTATCCCAAAACCTTGACTTGGCAAAAGTTCAAGGTACATTGGCAGATTTGTTGGGCTTTAAATTCGTGGAGGATACAGAAGCTGGAAGAGCCGTTAGGTTGCATAAGGAGATCATGAGAAAGGACAAACTCCTAATAATTGTGGACGATGTTTGGGAGAGAACAGAGTTGTCAAAGATAGGGATTCCCACCTACAATGAGCTTCAAAAGTGCAAGTCCAAAGTGCTACTCACCACCAGGAGATTGAACGTCTGTAATGCCATGAAGTGCCATGAAAAGATCAGCCTCAAAGTTCTCTCAGATCAAGATTCTTGGACCTTGTTCGTGAGAAATGCAGGAACAATTTCTTTGGAGCCCACCTCATTTGAGAAAGTGGCAAGGAGGGTAGCTTTAGAGTGTAAGGGTCTACCGATTGCATTGATAGCTGTTGCAAGGGCACTCGGAGATGAGGACCAGGAGGAATGGGAGAAGGCAGCTGAACGACTAGAGCAGTCGCAATATGTCAATCCCAACCATGAGGAAGATGAGGAAAATGCGTTCAGTTGTATACGATTAAGCTATGATTACTTGAAAAATGAAGACCACAAGTCATGCTTCTTGCTCTGTTGCCTGTTCCCAGAAGACCATGACATCGAAATAGAAGACTTGTTCCGGTACGCGATCGGAAAAAGATTGTTTCGAGATGCGAAAACAATGAAAGAAGCCAGAGGAAAATTAGTTACAGTGGTCAAGTACCTGAAAAATTCTAGCTTGCTTTTGGAAAGTGAGAAAGGACGTGTTAAGATGCATGATGTCATCCGGGATACAGCCATTCAAATTGCCGAATCTGAAGATTGCCATCGGTTTTTGGTGGAAGCTGGTCGTGGTTTAAAGGATTGGGGGCCGCACGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAGCGAAATTCACAAGCTACCCGAAGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTGCTAAACAGGAATTATGAACTAGATGAGATCCCCGAAAAGTTGATCCAGAATCTGAAAGAGTTAAGGGTCTTGGATCTTAGTAACACTAATATTTCTGTATTACCCCAATCATTTAGTCTCCTCACCAACCTCCTAGCTTTGTATTTAGATGATTGCGACGAATTAATTGACATTTCCGTTGTGGGGAAACTTAACAAGCTTGAAATTCTTAGTATGAGAAATTGTAACGGGGGGCAATTGTCGAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGACGTCAATTGCAATTGGCTACAAAAGCTGAAAGGAGGAATGGTTACAATTCCATCTGAAGTGATATCAAAGTTGCATAAATTAGAAGAATTGTACATGGTGTACTGTGGATTTGAGGACGGGCGGTGTGTAGCTGAGAAACCAGAAGAAGAAACCAATATTGTTAGGTTTGATGAGTTAGCTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCTTATCCGATAAAAGTTTCATCCCTAAAAATGTTGAGGTCGCACCAGATTGGGATTACTTTTGTATTAGTGTCTTCGGCAGATGCGCCAGCTCTGGATTCGGCTCCGGATTCGGCTCCGGTAAATACAAACAAGGAGATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATCAGTACCTTGCCTGATTGGTTTATCGAAGCAGTGACAGAGAAAACAGAGAAGCTAGAGTATTACTGCTGCAGAGGGATGAGAGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATTTGACAGTTGCTGGGAAATTAAGCATTTCACAGTACTTGAAAGAGTTGATGAACACAACAAGACGAGTTGAAACAGGACCCGTGTTTGAGAATTTGGAAGAGCTGCGTCTGATAAATCTGATCCAACTGGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTGTTTTATGTGCGTTTTGGTATATTTTTGAAGAGTGTATCAAAATTTGTACAGAGACTACCAAATCTGGAGAAACTAGTTTTAAAAACTGTATATAAATTGGAATATGTGTTCAGATGCGAAGGGGTCAAGCCAGAACAAGCAAAACTGAGAGAGATGCATTTGTTGTCTCTAAGAGCACTAAAAAGCGTATGTAATGGTCCTGCTCCACATGCAATGTTCCAGGGTCTTAAAATTTTGACCATTTACCGTTGCAAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTCAGTGTCTTGTTCAATTGGAAGACCTTCTTGTAGAGTGGTGTCCTTTGTTGGAAAGAGTAATGGAAGCAGTGAACAAGGAGAAGACTGTTCTACCAAAACTGAAGAATTTGGTTTTGAAGAATCTTCCTATTTTGTATGGTGCAATTGCTGCCGTTGATATTGAGTGTCCTTCGTTGGAACACTTGATGGTGGTGGATTGCCCCAAGCTTTTATTTTCAACCTCTTCCGATCTCTTCGAAGCCTTCAAAAGCAGGAACCAATTTTCATTTTCAGCCTCCACTTCTGACCCAGTCCAACTCGATGATCCACAACTATACCAAACTCTACGTGGCAGATCATCTAGGTTCCAATAA

Protein Analysis

1173

Amino Acids

133.41

Weight (kDa)

6.45

Isoelectric Point (pI)

41.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 68 - 231 9.1e-46 TIR domain
TIR_2 PF13676 71 - 166 1.8e-16 TIR domain
NB-ARC PF00931 251 - 415 4e-28 NB-ARC domain
LRR_8 PF13855 625 - 683 2.3e-09 Leucine rich repeat
LRR_14 PF23598 631 - 765 3.1e-18 Leucine-rich repeat region
LRR_RPS2 PF23247 929 - 1069 2.5e-17 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 293
AatII GACGTC 1 cut(s) 2178
Acc36I ACCTGC 1 cut(s) 2371
AccI GTMKAC 2 cut(s) 1312, 1453
AccII CGCG 2 cut(s) 267, 1565
AccIII TCCGGA 1 cut(s) 2492
AciI CCGC 4 cut(s) 41, 685, 1790, 2296
AclI AACGTT 1 cut(s) 296
AclWI GGATC 7 cut(s) 485, 1915, 1930, 1974, 2532, 2809, 3464
AcoI YGGCCR 1 cut(s) 609
AcuI CTGAAG 3 cut(s) 1755, 2250, 3287
AcyI GRCGYC 1 cut(s) 2175
AfaI GTAC 9 cut(s) 696, 943, 1562, 1640, 2267, 2276, 2569, 2729, 2925
AfiI CCNNNNNNNGG 5 cut(s) 78, 925, 2076, 2163, 2824
AflIII ACRYGT 2 cut(s) 850, 1681
AhdI GACNNNNNGTC 1 cut(s) 1492
AhlI ACTAGT 1 cut(s) 2950
AjiI CACGTC 1 cut(s) 1682
AjnI CCWGG 4 cut(s) 1142, 1359, 2851, 3096
AloI GAACNNNNNNTCC 4 cut(s) 1427, 1459, 2969, 3001
Alw21I GWGCWC 1 cut(s) 3055
Alw26I GTCTC 6 cut(s) 841, 2015, 2640, 2862, 2923, 3048
AlwI GGATC 7 cut(s) 485, 1915, 1930, 1974, 2532, 2809, 3464
AlwNI CAGNNNCTG 3 cut(s) 989, 1945, 2705
Ama87I CYCGRG 1 cut(s) 90
Aor13HI TCCGGA 1 cut(s) 2492
AoxI GGCC 3 cut(s) 69, 609, 1787
ApeKI GCWGC 9 cut(s) 593, 857, 1379, 1809, 2631, 2797, 3133, 3136, 3314
AseI ATTAAT 1 cut(s) 2057
Asp700I GAANNNNTTC 2 cut(s) 1249, 3189
AspLEI GCGC 2 cut(s) 778, 2474
AspS9I GGNCC 6 cut(s) 230, 1221, 1357, 1787, 2771, 3074
AsuC2I CCSGG 2 cut(s) 227, 1706
AsuHPI GGTGA 4 cut(s) 856, 1129, 2008, 2852
AsuII TTCGAA 2 cut(s) 525, 3402
AvaI CYCGRG 1 cut(s) 90
AvaII GGWCC 5 cut(s) 230, 1221, 1357, 2771, 3074
BaeGI GKGCMC 1 cut(s) 1345
BalI TGGCCA 1 cut(s) 611
BanII GRGCYC 1 cut(s) 1263
BbsI GAAGAC 6 cut(s) 1491, 1533, 1554, 2452, 3192, 3254
Bbv12I GWGCWC 1 cut(s) 3055
BbvI GCAGC 9 cut(s) 580, 844, 1391, 1796, 2618, 2784, 3120, 3123, 3301
BccI CCATC 5 cut(s) 52, 1752, 2233, 2570, 3346
BceAI ACGGC 4 cut(s) 672, 785, 983, 3302
BciT130I CCWGG 4 cut(s) 1144, 1361, 2853, 3098
BciVI GTATCC 4 cut(s) 322, 919, 973, 1702
BcnI CCSGG 2 cut(s) 227, 1706
BcoDI GTCTC 6 cut(s) 841, 2015, 2640, 2862, 2923, 3048
BcuI ACTAGT 1 cut(s) 2950
BfaI CTAG 8 cut(s) 599, 1391, 1653, 1913, 2027, 2618, 2951, 3507
BfmI CTRYAG 2 cut(s) 868, 2632
BfoI RGCGCY 1 cut(s) 779
BfuAI ACCTGC 1 cut(s) 2371
BfuI GTATCC 4 cut(s) 322, 919, 973, 1702
BglII AGATCT 1 cut(s) 313
BmcAI AGTACT 1 cut(s) 2729
Bme1390I CCNGG 6 cut(s) 227, 1144, 1361, 1706, 2853, 3098
Bme18I GGWCC 5 cut(s) 230, 1221, 1357, 2771, 3074
BmeRI GACNNNNNGTC 1 cut(s) 1492
BmeT110I CYCGRG 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 1682
BmgT120I GGNCC 6 cut(s) 230, 1221, 1357, 1787, 2771, 3074
BmiI GGNNCC 8 cut(s) 231, 1260, 1788, 2491, 2503, 2773, 3422, 3512
BmrFI CCNGG 6 cut(s) 227, 1144, 1361, 1706, 2853, 3098
BmrI ACTGGG 1 cut(s) 3449
BmsI GCATC 7 cut(s) 694, 1579, 1680, 2459, 2981, 3021, 3161
BmuI ACTGGG 1 cut(s) 3449
BpiI GAAGAC 6 cut(s) 1491, 1533, 1554, 2452, 3192, 3254
BplI GAGNNNNNCTC 4 cut(s) 580, 612, 1250, 1282
BpmI CTGGAG 3 cut(s) 1865, 2843, 2963
Bpu14I TTCGAA 2 cut(s) 525, 3402
BpuEI CTTGAG 2 cut(s) 2672, 2855
BpuMI CCSGG 2 cut(s) 227, 1706
BsaAI YACGTR 1 cut(s) 3494
BsaBI GATNNNNATC 1 cut(s) 1743
BsaHI GRCGYC 1 cut(s) 2175
BsaI GGTCTC 1 cut(s) 2862
BsaJI CCNNGG 5 cut(s) 226, 450, 2668, 2852, 3097
BsaWI WCCGGW 3 cut(s) 1557, 2492, 2504
BsaXI ACNNNNNCTCC 4 cut(s) 2199, 2229, 2816, 2846
Bsc4I CCNNNNNNNGG 5 cut(s) 78, 925, 2076, 2163, 2824
Bse1I ACTGG 2 cut(s) 2826, 3455
Bse3DI GCAATG 2 cut(s) 590, 3096
Bse8I GATNNNNATC 1 cut(s) 1743
BseAI TCCGGA 1 cut(s) 2492
BseBI CCWGG 4 cut(s) 1144, 1361, 2853, 3098
BseDI CCNNGG 5 cut(s) 226, 450, 2668, 2852, 3097
BseGI GGATG 5 cut(s) 63, 310, 1701, 2406, 2646
BseJI GATNNNNATC 1 cut(s) 1743
BseLI CCNNNNNNNGG 5 cut(s) 78, 925, 2076, 2163, 2824
BseMI GCAATG 2 cut(s) 590, 3096
BseMII CTCAG 5 cut(s) 633, 1218, 2298, 3014, 3179
BseNI ACTGG 2 cut(s) 2826, 3455
BseRI GAGGAG 4 cut(s) 532, 1846, 2003, 2840
BseSI GKGCMC 1 cut(s) 1345
BseXI GCAGC 9 cut(s) 580, 844, 1391, 1796, 2618, 2784, 3120, 3123, 3301
BseYI CCCAGC 1 cut(s) 2705
BsgI GTGCAG 2 cut(s) 843, 1795
Bsh1236I CGCG 2 cut(s) 267, 1565
Bsh1285I CGRYCG 2 cut(s) 271, 1569
BshFI GGCC 3 cut(s) 71, 611, 1789
BsiEI CGRYCG 2 cut(s) 271, 1569
BsiHKAI GWGCWC 1 cut(s) 3055
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 5 cut(s) 226, 1558, 1705, 2493, 2505
BslI CCNNNNNNNGG 5 cut(s) 78, 925, 2076, 2163, 2824
BsmAI GTCTC 6 cut(s) 841, 2015, 2640, 2862, 2923, 3048
BsmI GAATGC 1 cut(s) 163
BsnI GGCC 3 cut(s) 71, 611, 1789
Bso31I GGTCTC 1 cut(s) 2862
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 2 cut(s) 525, 3402
Bsp1286I GDGCHC 3 cut(s) 1263, 1345, 3055
Bsp13I TCCGGA 1 cut(s) 2492
Bsp1407I TGTACA 2 cut(s) 2265, 2923
Bsp19I CCATGG 2 cut(s) 450, 2668
BspACI CCGC 4 cut(s) 41, 685, 1790, 2296
BspANI GGCC 3 cut(s) 71, 611, 1789
BspCNI CTCAG 5 cut(s) 632, 1217, 2299, 3015, 3178
BspEI TCCGGA 1 cut(s) 2492
BspFNI CGCG 2 cut(s) 267, 1565
BspHI TCATGA 1 cut(s) 1020
BspLI GGNNCC 8 cut(s) 231, 1260, 1788, 2491, 2503, 2773, 3422, 3512
BspMAI CTGCAG 2 cut(s) 872, 2636
BspMI ACCTGC 1 cut(s) 2371
BspPI GGATC 7 cut(s) 485, 1915, 1930, 1974, 2532, 2809, 3464
BspQI GCTCTTC 2 cut(s) 988, 2787
BspT104I TTCGAA 2 cut(s) 525, 3402
BspTNI GGTCTC 1 cut(s) 2862
BsrDI GCAATG 2 cut(s) 590, 3096
BsrGI TGTACA 2 cut(s) 2265, 2923
BsrI ACTGG 2 cut(s) 2826, 3455
BssECI CCNNGG 5 cut(s) 226, 450, 2668, 2852, 3097
BssNAI GTATAC 1 cut(s) 1454
BssNI GRCGYC 1 cut(s) 2175
BssT1I CCWWGG 2 cut(s) 450, 2668
Bst1107I GTATAC 1 cut(s) 1454
Bst2UI CCWGG 4 cut(s) 1144, 1361, 2853, 3098
Bst4CI ACNGT 8 cut(s) 653, 1630, 2279, 2701, 2727, 2965, 3125, 3253
Bst6I CTCTTC 8 cut(s) 450, 509, 988, 1854, 2787, 2900, 3394, 3404
BstACI GRCGYC 1 cut(s) 2175
BstAPI GCANNNNNTGC 1 cut(s) 3314
BstAUI TGTACA 2 cut(s) 2265, 2923
BstBAI YACGTR 1 cut(s) 3494
BstBI TTCGAA 2 cut(s) 525, 3402
BstC8I GCNNGC 5 cut(s) 174, 868, 1658, 2476, 3131
BstDSI CCRYGG 2 cut(s) 450, 2668
BstF5I GGATG 5 cut(s) 63, 310, 1701, 2406, 2646
BstFNI CGCG 2 cut(s) 267, 1565
BstH2I RGCGCY 1 cut(s) 779
BstHHI GCGC 2 cut(s) 778, 2474
BstMAI GTCTC 6 cut(s) 841, 2015, 2640, 2862, 2923, 3048
BstMCI CGRYCG 2 cut(s) 271, 1569
BstNI CCWGG 4 cut(s) 1144, 1361, 2853, 3098
BstNSI RCATGY 2 cut(s) 854, 3089
BstSCI CCNGG 6 cut(s) 225, 1142, 1359, 1704, 2851, 3096
BstSFI CTRYAG 2 cut(s) 868, 2632
BstSLI GKGCMC 1 cut(s) 1345
BstUI CGCG 2 cut(s) 267, 1565
BstV1I GCAGC 9 cut(s) 580, 844, 1391, 1796, 2618, 2784, 3120, 3123, 3301
BstV2I GAAGAC 6 cut(s) 1491, 1533, 1554, 2452, 3192, 3254
BstX2I RGATCY 4 cut(s) 313, 1920, 1966, 2537
BstXI CCANNNNNNTGG 1 cut(s) 737
BstYI RGATCY 4 cut(s) 313, 1920, 1966, 2537
BstZ17I GTATAC 1 cut(s) 1454
BsuI GTATCC 4 cut(s) 322, 919, 973, 1702
BsuRI GGCC 3 cut(s) 71, 611, 1789
BtgI CCRYGG 2 cut(s) 450, 2668
BtrI CACGTC 1 cut(s) 1682
BtsCI GGATG 5 cut(s) 63, 310, 1701, 2406, 2646
BtsI GCAGTG 3 cut(s) 1812, 2601, 3240
BtsIMutI CAGTG 6 cut(s) 658, 1635, 1812, 2601, 3173, 3240
BveI ACCTGC 1 cut(s) 2371
Cac8I GCNNGC 5 cut(s) 174, 868, 1658, 2476, 3131
CaiI CAGNNNCTG 3 cut(s) 989, 1945, 2705
CciI TCATGA 1 cut(s) 1020
CfoI GCGC 2 cut(s) 778, 2474
Cfr13I GGNCC 6 cut(s) 230, 1221, 1357, 1787, 2771, 3074
CseI GACGC 2 cut(s) 89, 2789
Csp6I GTAC 9 cut(s) 695, 942, 1561, 1639, 2266, 2275, 2568, 2728, 2924
CspCI CAANNNNNGTGG 2 cut(s) 1252, 1287
CviQI GTAC 9 cut(s) 695, 942, 1561, 1639, 2266, 2275, 2568, 2728, 2924
DraI TTTAAA 3 cut(s) 967, 1776, 2958
DriI GACNNNNNGTC 1 cut(s) 1492
EaeI YGGCCR 1 cut(s) 609
Eam1104I CTCTTC 8 cut(s) 450, 509, 988, 1854, 2787, 2900, 3394, 3404
Eam1105I GACNNNNNGTC 1 cut(s) 1492
EarI CTCTTC 8 cut(s) 450, 509, 988, 1854, 2787, 2900, 3394, 3404
EciI GGCGGA 1 cut(s) 674
Eco130I CCWWGG 2 cut(s) 450, 2668
Eco24I GRGCYC 1 cut(s) 1263
Eco31I GGTCTC 1 cut(s) 2862
Eco32I GATATC 3 cut(s) 24, 509, 2238
Eco47I GGWCC 5 cut(s) 230, 1221, 1357, 2771, 3074
Eco57I CTGAAG 3 cut(s) 1755, 2250, 3287
Eco88I CYCGRG 1 cut(s) 90
EcoO109I RGGNCCY 2 cut(s) 230, 2771
EcoRI GAATTC 1 cut(s) 713
EcoRII CCWGG 4 cut(s) 1142, 1359, 2851, 3096
EcoRV GATATC 3 cut(s) 24, 509, 2238
EcoT14I CCWWGG 2 cut(s) 450, 2668
EcoT22I ATGCAT 2 cut(s) 1695, 3036
EcoT38I GRGCYC 1 cut(s) 1263
ErhI CCWWGG 2 cut(s) 450, 2668
FalI AAGNNNNNCTT 4 cut(s) 1484, 1516, 3177, 3209
FblI GTMKAC 2 cut(s) 1312, 1453
FokI GGATG 5 cut(s) 70, 317, 1688, 2393, 2653
FriOI GRGCYC 1 cut(s) 1263
FspBI CTAG 8 cut(s) 599, 1391, 1653, 1913, 2027, 2618, 2951, 3507
GlaI GCGC 2 cut(s) 777, 2473
GsaI CCCAGC 1 cut(s) 2709
GsuI CTGGAG 3 cut(s) 1865, 2843, 2963
HaeII RGCGCY 1 cut(s) 779
HaeIII GGCC 3 cut(s) 71, 611, 1789
HapII CCGG 5 cut(s) 226, 1558, 1705, 2493, 2505
HgaI GACGC 2 cut(s) 89, 2789
HhaI GCGC 2 cut(s) 778, 2474
Hin1I GRCGYC 1 cut(s) 2175
Hin6I GCGC 2 cut(s) 776, 2472
HinP1I GCGC 2 cut(s) 776, 2472
HincII GTYRAC 2 cut(s) 151, 664
HindII GTYRAC 2 cut(s) 151, 664
HindIII AAGCTT 3 cut(s) 531, 2090, 3371
HpaII CCGG 5 cut(s) 226, 1558, 1705, 2493, 2505
HphI GGTGA 4 cut(s) 856, 1129, 2008, 2852
Hpy166II GTNNAC 8 cut(s) 151, 656, 664, 1051, 1313, 1454, 2275, 3238
Hpy8I GTNNAC 8 cut(s) 151, 656, 664, 1051, 1313, 1454, 2275, 3238
Hpy99I CGWCG 3 cut(s) 105, 198, 2054
HpyAV CCTTC 8 cut(s) 568, 766, 1369, 1799, 2990, 3200, 3345, 3418
HpyCH4III ACNGT 8 cut(s) 653, 1630, 2279, 2701, 2727, 2965, 3125, 3253
HpyCH4IV ACGT 5 cut(s) 296, 1155, 1681, 2175, 3493
HpySE526I ACGT 5 cut(s) 296, 1155, 1681, 2175, 3493
Hsp92I GRCGYC 1 cut(s) 2175
HspAI GCGC 2 cut(s) 776, 2472
Kpn2I TCCGGA 1 cut(s) 2492
LguI GCTCTTC 2 cut(s) 988, 2787
LmnI GCTCC 8 cut(s) 580, 740, 1258, 1833, 2495, 2507, 2555, 3085
Lsp1109I GCAGC 9 cut(s) 580, 844, 1391, 1796, 2618, 2784, 3120, 3123, 3301
LweI GCATC 7 cut(s) 694, 1579, 1680, 2459, 2981, 3021, 3161
MaeI CTAG 8 cut(s) 599, 1391, 1653, 1913, 2027, 2618, 2951, 3507
MaeII ACGT 5 cut(s) 296, 1155, 1681, 2175, 3493
MaeIII GTNAC 9 cut(s) 49, 110, 1624, 1973, 2117, 2215, 2596, 2676, 2844
MfeI CAATTG 6 cut(s) 708, 2129, 2179, 2185, 3179, 3309
MflI RGATCY 4 cut(s) 313, 1920, 1966, 2537
MhlI GDGCHC 3 cut(s) 1263, 1345, 3055
MlsI TGGCCA 1 cut(s) 611
MluNI TGGCCA 1 cut(s) 611
MlyI GAGTC 3 cut(s) 82, 374, 640
MmeI TCCRAC 6 cut(s) 83, 808, 2842, 3195, 3321, 3484
Mox20I TGGCCA 1 cut(s) 611
Mph1103I ATGCAT 2 cut(s) 1695, 3036
MroI TCCGGA 1 cut(s) 2492
MroXI GAANNNNTTC 2 cut(s) 1249, 3189
MscI TGGCCA 1 cut(s) 611
MslI CAYNNNNRTG 1 cut(s) 2654
Msp20I TGGCCA 1 cut(s) 611
MspA1I CMGCKG 1 cut(s) 1382
MspI CCGG 5 cut(s) 226, 1558, 1705, 2493, 2505
MspR9I CCNGG 6 cut(s) 227, 1144, 1361, 1706, 2853, 3098
MunI CAATTG 6 cut(s) 708, 2129, 2179, 2185, 3179, 3309
Mva1269I GAATGC 1 cut(s) 163
MvaI CCWGG 4 cut(s) 1144, 1361, 2853, 3098
MvnI CGCG 2 cut(s) 267, 1565
NciI CCSGG 2 cut(s) 227, 1706
NcoI CCATGG 2 cut(s) 450, 2668
NlaIV GGNNCC 8 cut(s) 231, 1260, 1788, 2491, 2503, 2773, 3422, 3512
NmuCI GTSAC 2 cut(s) 49, 2596
NsiI ATGCAT 2 cut(s) 1695, 3036
NspI RCATGY 2 cut(s) 854, 3089
NspV TTCGAA 2 cut(s) 525, 3402
PaeR7I CTCGAG 1 cut(s) 90
PagI TCATGA 1 cut(s) 1020
PciI ACATGT 1 cut(s) 850
PciSI GCTCTTC 2 cut(s) 988, 2787
PcsI WCGNNNNNNNCGW 1 cut(s) 97
PctI GAATGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 1249, 3189
PflFI GACNNNGTC 1 cut(s) 3455
PfoI TCCNGGA 1 cut(s) 1704
Ple19I CGATCG 2 cut(s) 271, 1569
PleI GAGTC 3 cut(s) 82, 373, 640
PpsI GAGTC 3 cut(s) 82, 373, 640
Ppu21I YACGTR 1 cut(s) 3494
PpuMI RGGWCCY 2 cut(s) 230, 2771
PscI ACATGT 1 cut(s) 850
PshBI ATTAAT 1 cut(s) 2057
PsiI TTATAA 1 cut(s) 293
Psp1406I AACGTT 1 cut(s) 296
Psp5II RGGWCCY 2 cut(s) 230, 2771
Psp6I CCWGG 4 cut(s) 1142, 1359, 2851, 3096
PspFI CCCAGC 1 cut(s) 2705
PspGI CCWGG 4 cut(s) 1142, 1359, 2851, 3096
PspN4I GGNNCC 8 cut(s) 231, 1260, 1788, 2491, 2503, 2773, 3422, 3512
PspPI GGNCC 6 cut(s) 230, 1221, 1357, 1787, 2771, 3074
PspPPI RGGWCCY 2 cut(s) 230, 2771
PspXI VCTCGAGB 1 cut(s) 90
PstI CTGCAG 2 cut(s) 872, 2636
PstNI CAGNNNCTG 3 cut(s) 989, 1945, 2705
PsuI RGATCY 4 cut(s) 313, 1920, 1966, 2537
PsyI GACNNNGTC 1 cut(s) 3455
PvuI CGATCG 2 cut(s) 271, 1569
PvuII CAGCTG 1 cut(s) 1382
RsaI GTAC 9 cut(s) 696, 943, 1562, 1640, 2267, 2276, 2569, 2729, 2925
RsaNI GTAC 9 cut(s) 695, 942, 1561, 1639, 2266, 2275, 2568, 2728, 2924
RseI CAYNNNNRTG 1 cut(s) 2654
SapI GCTCTTC 2 cut(s) 988, 2787
Sau96I GGNCC 6 cut(s) 230, 1221, 1357, 1787, 2771, 3074
ScaI AGTACT 1 cut(s) 2729
SchI GAGTC 3 cut(s) 82, 374, 640
ScrFI CCNGG 6 cut(s) 227, 1144, 1361, 1706, 2853, 3098
SduI GDGCHC 3 cut(s) 1263, 1345, 3055
SfaNI GCATC 7 cut(s) 694, 1579, 1680, 2459, 2981, 3021, 3161
SfcI CTRYAG 2 cut(s) 868, 2632
Sfr274I CTCGAG 1 cut(s) 90
SfuI TTCGAA 2 cut(s) 525, 3402
SinI GGWCC 5 cut(s) 230, 1221, 1357, 2771, 3074
SlaI CTCGAG 1 cut(s) 90
SmiMI CAYNNNNRTG 1 cut(s) 2654
SmlI CTYRAG 3 cut(s) 90, 2687, 2870
SmoI CTYRAG 3 cut(s) 90, 2687, 2870
SpeI ACTAGT 1 cut(s) 2950
SsiI CCGC 4 cut(s) 41, 685, 1790, 2296
SspI AATATT 3 cut(s) 1984, 2332, 2376
SspMI CTAG 8 cut(s) 599, 1391, 1653, 1913, 2027, 2618, 2951, 3507
StyD4I CCNGG 6 cut(s) 225, 1142, 1359, 1704, 2851, 3096
StyI CCWWGG 2 cut(s) 450, 2668
TaaI ACNGT 8 cut(s) 653, 1630, 2279, 2701, 2727, 2965, 3125, 3253
TaiI ACGT 5 cut(s) 299, 1158, 1684, 2178, 3496
TatI WGTACW 4 cut(s) 2265, 2274, 2727, 2923
TauI GCSGC 1 cut(s) 1792
TscAI CASTG 6 cut(s) 658, 1635, 1819, 2601, 3173, 3240
TseFI GTSAC 2 cut(s) 49, 2596
TseI GCWGC 9 cut(s) 593, 857, 1379, 1809, 2631, 2797, 3133, 3136, 3314
Tsp45I GTSAC 2 cut(s) 49, 2596
TspGWI ACGGA 2 cut(s) 1809, 2059
TspRI CASTG 6 cut(s) 658, 1635, 1819, 2601, 3173, 3240
Tth111I GACNNNGTC 1 cut(s) 3455
VpaK11BI GGWCC 5 cut(s) 230, 1221, 1357, 2771, 3074
VspI ATTAAT 1 cut(s) 2057
XbaI TCTAGA 1 cut(s) 598
XceI RCATGY 2 cut(s) 854, 3089
XcmI CCANNNNNNNNNTGG 1 cut(s) 3491
XhoI CTCGAG 1 cut(s) 90
XmiI GTMKAC 2 cut(s) 1312, 1453
XmnI GAANNNNTTC 2 cut(s) 1249, 3189
XspI CTAG 8 cut(s) 599, 1391, 1653, 1913, 2027, 2618, 2951, 3507
ZraI GACGTC 1 cut(s) 2176
ZrmI AGTACT 1 cut(s) 2729
Zsp2I ATGCAT 2 cut(s) 1695, 3036
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.