RchiOBHm_Chr5g0079361

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000379
Physical Location & Seq
Reverse (-)
85036230 .. 85050858
14629 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35378

Sequence Viewer

Length: 3405 bp
ATGTCGGACTATCTTCACTCTCCGGCTGTAGAGCCGTTTAGTCAACAGCAGCGGCTCCAGGCGCTGATCGAGGGCGCCAGGGAGAACTGGACCTATGCCATCTTCTGGCAGTCGTCGTACGACATGTCGGGAGATTCCGTTCTCGGGTGGGGCGACGGGTTCTACAAGGACGAGAGTGATAAATGGAAAGCGAGGCCCAAAAAGATGACGTCATTAGTCGAGCAGGAGCACCGGAAGAAAGTGCTCAGGGAACTCAACTCTTTGATTTCTGGGGCCCACACCACCGCCGACGACGAGGTCGTAGATCAAGTCACTGGTACAGAATGGTTCTTCCTTGTTTCCATGACCCATGGTTTCCTCAACGGCGGCGGGTTGCCGGGTCAGGCCTTTTTCTATTCCAACCCGGTCTGGGTCACCGGGCCGGACCGCCTAGCGGCGTCCTCCTGCGAGCGGGCCCGCCAAGCTCAGGTCTCCGGCCTACAGACCATGGTCTGCGTCCCGACGGCGAATGGGGTGGTCGAATTGGGCTCGACGGAGCTGATATTCCAAAGCTCGGATGTGATGAACGAGGCCCGAGTATTGTTTGATTTCAATAATCTTGAAGTCGGTTCTTGGCCTGTAGCTCCGGCCACTTCAGGACCGAGTAGCAGTAACCATCACATCTCTAAGCCGATTCAGTTTGACAACCACCCTAGTTCCAGTAGCTCTTCTGCTACTCAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTGTAGCTGATTTATACGATCGGCTGCAAAACAAGAGAGGAATTAGAACATTTATGGATGATCCAGATCTTCGAGTAGGGGATGCTATTTCTCCCGCTCTCTTAAAGGCGATTGAAGAATCAAGGTTTGCAATCGTTGTTCTATCGAAAAATTATGCTCATTCTACTTGGTGTTTGGAGGAACTTACAAGTATCTGTCAGTGCATGAAAGACTTCAACAGAATTCTTCCACTTTTCTATAATGTGGAACCCTCTGATGTACGGTACCGGAAAAGGAGTTTCCGAAGAGCTTTCGATAAGCATGAACGCTCTGGGCGACACACATCAGAGAAGGTGCAGCGGTGGAATGATGCGTTAAACAAAGTGGCCAGTTTTTCTGGGTGGCATACAAAGAATTTTAAGAGTGATAGAGATCTTGTTGACTCCATTGTGGAATTTGTGTGCAGTAAAGTACAACCTATTGAAATCGGTTTTACAATGTTCGCCGGAGATTTTCAAGCATTTGAAGCAACAAGAAAAAGCATGAATGAGGTTATGAAGGTGCTTCAAGATGACGAGGTCACTGCCCTTGGGGTCTATGGAATGGGAGGGGTCGGCAAGACAACCATGGTTAAACGTGTCGGCGCGCTAGCCCGCAAGAACGGAATTTTTAACCATGTACTTCTGGCTGTCATATCCCAAAGCCCTGACTCGAGAAATATTCAAGCCACATTGGCAGAGCTGCTGGGCTTCAAATTCAAGTGGGAGACAGAAATTGGAAGAGCCGCTGAGTTGCATGAGAAGATAATGAGTAAAAATAAGATCCTTATAATCCTGGACGACATGTGGGAGAGAATGGATTTGTCAAGAATAGGAATTCCCAGCTATGACGAGCTCAAAAAATACAATTCTAAAGTCCTACTCACCACGAGGAGATTGCATGTTTGTAATTCCATGGACAGCCAAGCAAGCATTCCTCTCGATATCTTATCAGAAGAAGATTCTTGGAAATTGTTTATGAGAAATGCAGGAAGGTCTTTTGAATCCTCCAATTTTAATAACGTAGCAAGAAAGGTAGCTGCAGAATGTAGGGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGCACTCAGAGATAAAGATCTGGCGGAATGGCAAAAAGCAGCTCAACGACTGGAGAAGTCGCAAATTGCCAACCCTGATGATAAGGGAGATGCATTCGAATGTATAAAATTAAGCTTTGATTACTTGAAAGATGAGGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATTCCCAGAAGACCATGAAATCAATATAGAAGACTTGTTCAGGTATGCGATTGGAAAAGGATTGTTTCAAGATGCCGAAACAATGTACGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGAGAGTGAATACATTGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATGAATATTGCAAAATCTAAAGATGGGCATCGGTTTACGGTAAAAGTTGGCTGTGGTTTAGAGGATTGGCGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAAAATTCGCAAGCTACCCGAAGAAGAGTTGGTTTGCATATCTGATGCTAAATGCACACCTAAATGTCTTGAGGTCAATGGAGAATATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATAAGTACCTTACCTGACTGGTTTATCAACACAGTGACAAAGAAAACAGAGAAGCTAGAGTACAGTCGCTGCAAAGGGATGAGTGACATTCTTATGGAATATGACCATGGGAGGTTACATAAGCTCAAGCATCTTAGAGTATGTGGTTATTTTTGTGATTCCTATGTGTACTTGAAGGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTGTGAATTTGGAAGAGTTGCATCTGATAGATCTGATCCACCTTAATGAGTTGTGTGTTGGAGAGTTACCACATGGGTCTCTCTTCAATCTGAAGGTATTGCATATATCTTTTTGTAATATTTTGAAGAATGTATCAAAACTGGTACAGAGACTACCAAATCTGGAGAGACTATATTTAAATTGCATGGATGACCTGGAATATGTGTTTGGATGTGAAGGGTTTGAGCCGAAAGAATCAAAACTGAGAGAGATGCAGTTGTTGGGTCCAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCCAGAGTCTTAAAAGTTTGACTTTTTACCGTTGCGAGTTGCTGCAAAGTCTGTTCGCATATGATGTAGCCCAGAATCTTGTTCAATTGGAAGACCTTTTAGTAGAATGGTGCCCTTTAATGAAGAACTTGGTTTTCATGGGACTTCCCAAGTTGTATGGTGCAAGTGCTACTGTTGACATTGAGTGTCCTTCATTAGAACACTTGATTGTGGTGGATTGTCCCCGGTTTTCATTTTCAACCTCTTCCTTAAGCAGGAACCCGTCTTCATTTTCAACCTCTGCTTCCGACTACTTTGGCAGCACCAACCTAGTCAAACTCAATGATCAACAACATCTTCAACTTCTATCATCAAGGTTGATGAACTTGTACAGGCTCAGTTAA

Protein Analysis

1134

Amino Acids

128.64

Weight (kDa)

6.52

Isoelectric Point (pI)

45.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-MYC_N PF14215 16 - 195 5.7e-53 bHLH-MYC and R2R3-MYB transcription factors N-terminal
TIR PF01582 242 - 409 3.5e-46 TIR domain
TIR_2 PF13676 245 - 342 7.4e-17 TIR domain
NB-ARC PF00931 430 - 594 1.4e-28 NB-ARC domain
LRR_RPS2 PF23247 906 - 1045 3.7e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1580
AasI GACNNNNNNGTC 1 cut(s) 296
AatII GACGTC 1 cut(s) 212
Acc65I GGTACC 1 cut(s) 1035
AccB1I GGYRCC 3 cut(s) 74, 1035, 3132
AccB7I CCANNNNNTGG 1 cut(s) 105
AccBSI CCGCTC 2 cut(s) 451, 869
AccI GTMKAC 1 cut(s) 1846
AccII CGCG 3 cut(s) 1397, 2322, 3015
AclWI GGATC 4 cut(s) 827, 1567, 2457, 2737
AcoI YGGCCR 3 cut(s) 627, 1137, 2315
AcsI RAATTY 9 cut(s) 993, 1165, 1205, 1416, 1505, 1626, 2367, 2713, 2977
AcuI CTGAAG 2 cut(s) 618, 2819
AcyI GRCGYC 3 cut(s) 75, 209, 437
AfiI CCNNNNNNNGG 8 cut(s) 105, 144, 409, 433, 450, 466, 553, 3276
AflII CTTAAG 1 cut(s) 3271
AflIII ACRYGT 4 cut(s) 123, 1387, 1593, 3013
AhdI GACNNNNNGTC 1 cut(s) 2017
AjnI CCWGG 4 cut(s) 57, 77, 1584, 2901
AjuI GAANNNNNNNTTGG 4 cut(s) 1707, 1739, 2898, 2930
AloI GAACNNNNNNTCC 2 cut(s) 123, 155
Alw21I GWGCWC 3 cut(s) 231, 246, 1647
Alw26I GTCTC 5 cut(s) 475, 1511, 2790, 2851, 2869
AlwI GGATC 4 cut(s) 827, 1567, 2457, 2737
AlwNI CAGNNNCTG 2 cut(s) 64, 2556
Ama87I CYCGRG 3 cut(s) 143, 573, 1462
ApaI GGGCCC 2 cut(s) 277, 457
ApoI RAATTY 9 cut(s) 993, 1165, 1205, 1416, 1505, 1626, 2367, 2713, 2977
Asp700I GAANNNNTTC 1 cut(s) 983
Asp718I GGTACC 1 cut(s) 1035
AspLEI GCGC 4 cut(s) 64, 77, 1397, 1399
AsuC2I CCSGG 5 cut(s) 378, 404, 418, 2234, 3247
AsuHPI GGTGA 2 cut(s) 406, 1666
AsuII TTCGAA 1 cut(s) 1971
AsuNHI GCTAGC 1 cut(s) 1399
AvaI CYCGRG 3 cut(s) 143, 573, 1462
AvaII GGWCC 8 cut(s) 90, 424, 638, 752, 2011, 2699, 2972, 3002
BaeGI GKGCMC 4 cut(s) 277, 457, 1879, 3137
BalI TGGCCA 1 cut(s) 1139
BanI GGYRCC 3 cut(s) 74, 1035, 3132
BanII GRGCYC 4 cut(s) 277, 457, 530, 1647
BarI GAAGNNNNNNTAC 2 cut(s) 2531, 2563
BauI CACGAG 1 cut(s) 1678
BbsI GAAGAC 4 cut(s) 2058, 2079, 3120, 3279
Bbv12I GWGCWC 3 cut(s) 231, 246, 1647
BccI CCATC 3 cut(s) 107, 663, 2261
BceAI ACGGC 3 cut(s) 19, 379, 519
BcgI CGANNNNNNTGC 6 cut(s) 1316, 1350, 1669, 1703, 1711, 1745
BciT130I CCWGG 4 cut(s) 59, 79, 1586, 2903
BciVI GTATCC 1 cut(s) 2230
BclI TGATCA 1 cut(s) 3346
BcnI CCSGG 5 cut(s) 378, 404, 418, 2234, 3247
BcoDI GTCTC 5 cut(s) 475, 1511, 2790, 2851, 2869
BfaI CTAG 6 cut(s) 431, 693, 1400, 2178, 2543, 3332
BfmI CTRYAG 4 cut(s) 27, 479, 618, 1830
BfoI RGCGCY 2 cut(s) 65, 78
BfrI CTTAAG 1 cut(s) 3271
BfuI GTATCC 1 cut(s) 2230
BglI GCCNNNNNGGC 1 cut(s) 1484
BglII AGATCT 4 cut(s) 838, 1183, 1891, 2737
Bme1390I CCNGG 9 cut(s) 59, 79, 378, 404, 418, 1586, 2234, 2903, 3247
Bme18I GGWCC 8 cut(s) 90, 424, 638, 752, 2011, 2699, 2972, 3002
BmeRI GACNNNNNGTC 1 cut(s) 2017
BmeT110I CYCGRG 3 cut(s) 143, 573, 1462
BmrFI CCNGG 9 cut(s) 59, 79, 378, 404, 418, 1586, 2234, 2903, 3247
BmtI GCTAGC 1 cut(s) 1403
BoxI GACNNNNGTC 1 cut(s) 488
BpiI GAAGAC 4 cut(s) 2058, 2079, 3120, 3279
BpmI CTGGAG 3 cut(s) 41, 1946, 2891
Bpu10I CCTNAGC 2 cut(s) 245, 465
Bpu14I TTCGAA 1 cut(s) 1971
BpuEI CTTGAG 2 cut(s) 2453, 2597
BpuMI CCSGG 5 cut(s) 378, 404, 418, 2234, 3247
BsaBI GATNNNNATC 4 cut(s) 837, 1182, 1890, 2271
BsaHI GRCGYC 3 cut(s) 75, 209, 437
BsaI GGTCTC 2 cut(s) 475, 2790
BsaJI CCNNGG 8 cut(s) 78, 349, 486, 1339, 1377, 1704, 2593, 3245
BsaWI WCCGGW 2 cut(s) 231, 1038
BsaXI ACNNNNNCTCC 2 cut(s) 123, 153
Bsc4I CCNNNNNNNGG 8 cut(s) 105, 144, 409, 433, 450, 466, 553, 3276
Bse1I ACTGG 8 cut(s) 92, 319, 699, 1140, 1929, 2510, 2702, 2853
Bse8I GATNNNNATC 4 cut(s) 837, 1182, 1890, 2271
BseBI CCWGG 4 cut(s) 59, 79, 1586, 2903
BseDI CCNNGG 8 cut(s) 78, 349, 486, 1339, 1377, 1704, 2593, 3245
BseGI GGATG 8 cut(s) 562, 835, 859, 2213, 2229, 2571, 2902, 2924
BseJI GATNNNNATC 4 cut(s) 837, 1182, 1890, 2271
BseLI CCNNNNNNNGG 8 cut(s) 105, 144, 409, 433, 450, 466, 553, 3276
BseMII CTCAG 6 cut(s) 259, 479, 731, 1530, 1894, 2942
BseNI ACTGG 8 cut(s) 92, 319, 699, 1140, 1929, 2510, 2702, 2853
BsePI GCGCGC 1 cut(s) 1395
BseRI GAGGAG 1 cut(s) 1696
BseSI GKGCMC 4 cut(s) 277, 457, 1879, 3137
BseYI CCCAGC 2 cut(s) 1495, 1631
BsgI GTGCAG 3 cut(s) 1127, 1234, 2323
Bsh1236I CGCG 3 cut(s) 1397, 2322, 3015
Bsh1285I CGRYCG 1 cut(s) 793
BshNI GGYRCC 3 cut(s) 74, 1035, 3132
BsiEI CGRYCG 1 cut(s) 793
BsiHKAI GWGCWC 3 cut(s) 231, 246, 1647
BsiHKCI CYCGRG 3 cut(s) 143, 573, 1462
BsiWI CGTACG 1 cut(s) 117
BslFI GGGAC 3 cut(s) 482, 3177, 3228
BslI CCNNNNNNNGG 8 cut(s) 105, 144, 409, 433, 450, 466, 553, 3276
BsmAI GTCTC 5 cut(s) 475, 1511, 2790, 2851, 2869
BsmFI GGGAC 3 cut(s) 482, 3177, 3228
BsmI GAATGC 2 cut(s) 1722, 1967
Bso31I GGTCTC 2 cut(s) 475, 2790
BsoBI CYCGRG 3 cut(s) 143, 573, 1462
Bsp119I TTCGAA 1 cut(s) 1971
Bsp120I GGGCCC 2 cut(s) 273, 453
Bsp1286I GDGCHC 8 cut(s) 231, 246, 277, 457, 530, 1647, 1879, 3137
Bsp1407I TGTACA 1 cut(s) 3390
Bsp19I CCATGG 5 cut(s) 349, 486, 1377, 1704, 2593
BspCNI CTCAG 6 cut(s) 258, 478, 730, 1531, 1893, 2943
BspFNI CGCG 3 cut(s) 1397, 2322, 3015
BspMAI CTGCAG 1 cut(s) 1834
BspOI GCTAGC 1 cut(s) 1403
BspPI GGATC 4 cut(s) 827, 1567, 2457, 2737
BspQI GCTCTTC 4 cut(s) 712, 1051, 1525, 2981
BspT104I TTCGAA 1 cut(s) 1971
BspT107I GGYRCC 3 cut(s) 74, 1035, 3132
BspTI CTTAAG 1 cut(s) 3271
BspTNI GGTCTC 2 cut(s) 475, 2790
BsrBI CCGCTC 2 cut(s) 451, 869
BsrGI TGTACA 1 cut(s) 3390
BsrI ACTGG 8 cut(s) 92, 319, 699, 1140, 1929, 2510, 2702, 2853
BssECI CCNNGG 8 cut(s) 78, 349, 486, 1339, 1377, 1704, 2593, 3245
BssHII GCGCGC 1 cut(s) 1395
BssNI GRCGYC 3 cut(s) 75, 209, 437
BssSI CACGAG 1 cut(s) 1678
BssT1I CCWWGG 6 cut(s) 349, 486, 1339, 1377, 1704, 2593
Bst2BI CACGAG 1 cut(s) 1678
Bst2UI CCWGG 4 cut(s) 59, 79, 1586, 2903
Bst4CI ACNGT 6 cut(s) 1035, 2284, 2521, 2552, 3053, 3196
Bst6I CTCTTC 8 cut(s) 712, 1051, 1525, 2382, 2715, 2795, 2981, 3271
BstACI GRCGYC 3 cut(s) 75, 209, 437
BstAFI CTTAAG 1 cut(s) 3271
BstAUI TGTACA 1 cut(s) 3390
BstBI TTCGAA 1 cut(s) 1971
BstC8I GCNNGC 9 cut(s) 449, 453, 457, 1397, 1401, 1405, 1720, 2183, 2375
BstDEI CTNAG 9 cut(s) 245, 465, 666, 717, 1539, 1880, 2621, 2951, 3398
BstDSI CCRYGG 5 cut(s) 349, 486, 1377, 1704, 2593
BstEII GGTNACC 1 cut(s) 412
BstENI CCTNNNNNAGG 1 cut(s) 3274
BstF5I GGATG 8 cut(s) 562, 835, 859, 2213, 2229, 2571, 2902, 2924
BstFNI CGCG 3 cut(s) 1397, 2322, 3015
BstH2I RGCGCY 2 cut(s) 65, 78
BstHHI GCGC 4 cut(s) 64, 77, 1397, 1399
BstMAI GTCTC 5 cut(s) 475, 1511, 2790, 2851, 2869
BstMCI CGRYCG 1 cut(s) 793
BstMWI GCNNNNNNNGC 6 cut(s) 61, 461, 1277, 1484, 1719, 1862
BstNI CCWGG 4 cut(s) 59, 79, 1586, 2903
BstNSI RCATGY 3 cut(s) 127, 1597, 1694
BstPAI GACNNNNGTC 1 cut(s) 488
BstPI GGTNACC 1 cut(s) 412
BstSCI CCNGG 9 cut(s) 57, 77, 376, 402, 416, 1584, 2232, 2901, 3245
BstSFI CTRYAG 4 cut(s) 27, 479, 618, 1830
BstSLI GKGCMC 4 cut(s) 277, 457, 1879, 3137
BstUI CGCG 3 cut(s) 1397, 2322, 3015
BstV2I GAAGAC 4 cut(s) 2058, 2079, 3120, 3279
BstX2I RGATCY 6 cut(s) 838, 1183, 1572, 1891, 2462, 2737
BstYI RGATCY 6 cut(s) 838, 1183, 1572, 1891, 2462, 2737
BsuI GTATCC 1 cut(s) 2230
BtgI CCRYGG 5 cut(s) 349, 486, 1377, 1704, 2593
BtsCI GGATG 8 cut(s) 562, 835, 859, 2213, 2229, 2571, 2902, 2924
BtsI GCAGTG 2 cut(s) 1332, 2340
BtsIMutI CAGTG 9 cut(s) 312, 725, 977, 1332, 2160, 2340, 2526, 2709, 2988
Cac8I GCNNGC 9 cut(s) 449, 453, 457, 1397, 1401, 1405, 1720, 2183, 2375
CaiI CAGNNNCTG 2 cut(s) 64, 2556
CfoI GCGC 4 cut(s) 64, 77, 1397, 1399
CpoI CGGWCCG 1 cut(s) 424
CseI GACGC 2 cut(s) 426, 484
CspI CGGWCCG 1 cut(s) 424
DdeI CTNAG 9 cut(s) 245, 465, 666, 717, 1539, 1880, 2621, 2951, 3398
DinI GGCGCC 1 cut(s) 76
DraI TTTAAA 1 cut(s) 2886
DrdI GACNNNNNNGTC 1 cut(s) 296
DriI GACNNNNNGTC 1 cut(s) 2017
DseDI GACNNNNNNGTC 1 cut(s) 296
EaeI YGGCCR 3 cut(s) 627, 1137, 2315
Eam1104I CTCTTC 8 cut(s) 712, 1051, 1525, 2382, 2715, 2795, 2981, 3271
Eam1105I GACNNNNNGTC 1 cut(s) 2017
EarI CTCTTC 8 cut(s) 712, 1051, 1525, 2382, 2715, 2795, 2981, 3271
EciI GGCGGA 1 cut(s) 1913
Ecl136II GAGCTC 1 cut(s) 1645
Eco130I CCWWGG 6 cut(s) 349, 486, 1339, 1377, 1704, 2593
Eco147I AGGCCT 1 cut(s) 386
Eco24I GRGCYC 4 cut(s) 277, 457, 530, 1647
Eco31I GGTCTC 2 cut(s) 475, 2790
Eco32I GATATC 1 cut(s) 1735
Eco47I GGWCC 8 cut(s) 90, 424, 638, 752, 2011, 2699, 2972, 3002
Eco53kI GAGCTC 1 cut(s) 1645
Eco57I CTGAAG 2 cut(s) 618, 2819
Eco88I CYCGRG 3 cut(s) 143, 573, 1462
Eco91I GGTNACC 1 cut(s) 412
EcoICRI GAGCTC 1 cut(s) 1645
EcoNI CCTNNNNNAGG 1 cut(s) 3274
EcoO109I RGGNCCY 2 cut(s) 273, 752
EcoO65I GGTNACC 1 cut(s) 412
EcoRI GAATTC 2 cut(s) 993, 1626
EcoRII CCWGG 4 cut(s) 57, 77, 1584, 2901
EcoRV GATATC 1 cut(s) 1735
EcoT14I CCWWGG 6 cut(s) 349, 486, 1339, 1377, 1704, 2593
EcoT22I ATGCAT 2 cut(s) 1969, 2223
EcoT38I GRGCYC 4 cut(s) 277, 457, 530, 1647
EgeI GGCGCC 1 cut(s) 76
EheI GGCGCC 1 cut(s) 76
ErhI CCWWGG 6 cut(s) 349, 486, 1339, 1377, 1704, 2593
FalI AAGNNNNNCTT 4 cut(s) 2009, 2041, 3028, 3060
FaqI GGGAC 3 cut(s) 482, 3177, 3228
FauI CCCGC 5 cut(s) 362, 444, 464, 874, 1412
FauNDI CATATG 1 cut(s) 3082
FbaI TGATCA 1 cut(s) 3346
FblI GTMKAC 1 cut(s) 1846
FokI GGATG 8 cut(s) 569, 842, 866, 2216, 2220, 2578, 2909, 2931
FriOI GRGCYC 4 cut(s) 277, 457, 530, 1647
FspBI CTAG 6 cut(s) 431, 693, 1400, 2178, 2543, 3332
GlaI GCGC 4 cut(s) 63, 76, 1396, 1398
GsaI CCCAGC 2 cut(s) 1499, 1635
GsuI CTGGAG 3 cut(s) 41, 1946, 2891
HaeII RGCGCY 2 cut(s) 65, 78
HgaI GACGC 2 cut(s) 426, 484
HhaI GCGC 4 cut(s) 64, 77, 1397, 1399
Hin1I GRCGYC 3 cut(s) 75, 209, 437
Hin6I GCGC 4 cut(s) 62, 75, 1395, 1397
HinP1I GCGC 4 cut(s) 62, 75, 1395, 1397
HincII GTYRAC 3 cut(s) 44, 1192, 3199
HindII GTYRAC 3 cut(s) 44, 1192, 3199
HindIII AAGCTT 1 cut(s) 1987
HphI GGTGA 2 cut(s) 406, 1666
Hpy166II GTNNAC 6 cut(s) 44, 1192, 1847, 2280, 2656, 3199
Hpy8I GTNNAC 6 cut(s) 44, 1192, 1847, 2280, 2656, 3199
Hpy99I CGWCG 6 cut(s) 118, 158, 293, 296, 505, 535
HpyAV CCTTC 9 cut(s) 759, 1096, 1303, 1776, 2327, 2656, 2794, 2918, 3222
HpyCH4III ACNGT 6 cut(s) 1035, 2284, 2521, 2552, 3053, 3196
HpyCH4IV ACGT 3 cut(s) 209, 1387, 1812
HpyF10VI GCNNNNNNNGC 6 cut(s) 61, 461, 1277, 1484, 1719, 1862
HpyF3I CTNAG 9 cut(s) 245, 465, 666, 717, 1539, 1880, 2621, 2951, 3398
HpySE526I ACGT 3 cut(s) 209, 1387, 1812
Hsp92I GRCGYC 3 cut(s) 75, 209, 437
HspAI GCGC 4 cut(s) 62, 75, 1395, 1397
KasI GGCGCC 1 cut(s) 74
KpnI GGTACC 1 cut(s) 1039
Ksp22I TGATCA 1 cut(s) 3346
LguI GCTCTTC 4 cut(s) 712, 1051, 1525, 2981
LmnI GCTCC 5 cut(s) 60, 226, 535, 628, 2480
MaeI CTAG 6 cut(s) 431, 693, 1400, 2178, 2543, 3332
MaeII ACGT 3 cut(s) 209, 1387, 1812
MaeIII GTNAC 8 cut(s) 310, 412, 650, 1330, 2521, 2570, 2601, 2772
MbiI CCGCTC 2 cut(s) 451, 869
MfeI CAATTG 2 cut(s) 1851, 3107
MflI RGATCY 6 cut(s) 838, 1183, 1572, 1891, 2462, 2737
MhlI GDGCHC 8 cut(s) 231, 246, 277, 457, 530, 1647, 1879, 3137
MlsI TGGCCA 1 cut(s) 1139
MluI ACGCGT 1 cut(s) 3013
MluNI TGGCCA 1 cut(s) 1139
Mly113I GGCGCC 1 cut(s) 75
MlyI GAGTC 3 cut(s) 1187, 1454, 3037
MmeI TCCRAC 3 cut(s) 423, 2746, 3333
Mox20I TGGCCA 1 cut(s) 1139
Mph1103I ATGCAT 2 cut(s) 1969, 2223
MroXI GAANNNNTTC 1 cut(s) 983
MscI TGGCCA 1 cut(s) 1139
MslI CAYNNNNRTG 4 cut(s) 1972, 2425, 2579, 2751
Msp20I TGGCCA 1 cut(s) 1139
MspA1I CMGCKG 3 cut(s) 52, 1111, 1538
MspCI CTTAAG 1 cut(s) 3271
MspR9I CCNGG 9 cut(s) 59, 79, 378, 404, 418, 1586, 2234, 2903, 3247
MunI CAATTG 2 cut(s) 1851, 3107
Mva1269I GAATGC 2 cut(s) 1722, 1967
MvaI CCWGG 4 cut(s) 59, 79, 1586, 2903
MvnI CGCG 3 cut(s) 1397, 2322, 3015
MwoI GCNNNNNNNGC 6 cut(s) 61, 461, 1277, 1484, 1719, 1862
NarI GGCGCC 1 cut(s) 75
NciI CCSGG 5 cut(s) 378, 404, 418, 2234, 3247
NcoI CCATGG 5 cut(s) 349, 486, 1377, 1704, 2593
NdeI CATATG 1 cut(s) 3082
NheI GCTAGC 1 cut(s) 1399
NmuCI GTSAC 5 cut(s) 310, 412, 1330, 2521, 2570
NsiI ATGCAT 2 cut(s) 1969, 2223
NspI RCATGY 3 cut(s) 127, 1597, 1694
NspV TTCGAA 1 cut(s) 1971
PaeR7I CTCGAG 1 cut(s) 1462
PauI GCGCGC 1 cut(s) 1395
PceI AGGCCT 1 cut(s) 386
PciI ACATGT 2 cut(s) 123, 1593
PciSI GCTCTTC 4 cut(s) 712, 1051, 1525, 2981
PcsI WCGNNNNNNNCGW 3 cut(s) 150, 297, 1084
PctI GAATGC 2 cut(s) 1722, 1967
PdmI GAANNNNTTC 1 cut(s) 983
Pfl23II CGTACG 1 cut(s) 117
PflFI GACNNNGTC 2 cut(s) 296, 1328
PflMI CCANNNNNTGG 1 cut(s) 105
PfoI TCCNGGA 2 cut(s) 1584, 2232
Ple19I CGATCG 1 cut(s) 793
PleI GAGTC 3 cut(s) 1187, 1454, 3036
PluTI GGCGCC 1 cut(s) 78
PpsI GAGTC 3 cut(s) 1187, 1454, 3036
PpuMI RGGWCCY 1 cut(s) 752
PscI ACATGT 2 cut(s) 123, 1593
PshAI GACNNNNGTC 1 cut(s) 488
PsiI TTATAA 1 cut(s) 1580
Psp124BI GAGCTC 1 cut(s) 1647
Psp5II RGGWCCY 1 cut(s) 752
Psp6I CCWGG 4 cut(s) 57, 77, 1584, 2901
PspEI GGTNACC 1 cut(s) 412
PspFI CCCAGC 2 cut(s) 1495, 1631
PspGI CCWGG 4 cut(s) 57, 77, 1584, 2901
PspLI CGTACG 1 cut(s) 117
PspOMI GGGCCC 2 cut(s) 273, 453
PspPPI RGGWCCY 1 cut(s) 752
PstI CTGCAG 1 cut(s) 1834
PstNI CAGNNNCTG 2 cut(s) 64, 2556
PsuI RGATCY 6 cut(s) 838, 1183, 1572, 1891, 2462, 2737
PsyI GACNNNGTC 2 cut(s) 296, 1328
PteI GCGCGC 1 cut(s) 1395
PvuI CGATCG 1 cut(s) 793
RseI CAYNNNNRTG 4 cut(s) 1972, 2425, 2579, 2751
Rsr2I CGGWCCG 1 cut(s) 424
RsrII CGGWCCG 1 cut(s) 424
SacI GAGCTC 1 cut(s) 1647
SapI GCTCTTC 4 cut(s) 712, 1051, 1525, 2981
SchI GAGTC 3 cut(s) 1187, 1454, 3037
ScrFI CCNGG 9 cut(s) 59, 79, 378, 404, 418, 1586, 2234, 2903, 3247
SduI GDGCHC 8 cut(s) 231, 246, 277, 457, 530, 1647, 1879, 3137
SfcI CTRYAG 4 cut(s) 27, 479, 618, 1830
SfoI GGCGCC 1 cut(s) 76
Sfr274I CTCGAG 1 cut(s) 1462
SfuI TTCGAA 1 cut(s) 1971
SinI GGWCC 8 cut(s) 90, 424, 638, 752, 2011, 2699, 2972, 3002
SlaI CTCGAG 1 cut(s) 1462
SmiI ATTTAAAT 1 cut(s) 2886
SmiMI CAYNNNNRTG 4 cut(s) 1972, 2425, 2579, 2751
SmlI CTYRAG 4 cut(s) 1462, 2432, 2612, 3271
SmoI CTYRAG 4 cut(s) 1462, 2432, 2612, 3271
SseBI AGGCCT 1 cut(s) 386
SspDI GGCGCC 1 cut(s) 74
SspI AATATT 3 cut(s) 1471, 2251, 2827
SspMI CTAG 6 cut(s) 431, 693, 1400, 2178, 2543, 3332
SstI GAGCTC 1 cut(s) 1647
StuI AGGCCT 1 cut(s) 386
StyD4I CCNGG 9 cut(s) 57, 77, 376, 402, 416, 1584, 2232, 2901, 3245
StyI CCWWGG 6 cut(s) 349, 486, 1339, 1377, 1704, 2593
SwaI ATTTAAAT 1 cut(s) 2886
TaaI ACNGT 6 cut(s) 1035, 2284, 2521, 2552, 3053, 3196
TaiI ACGT 3 cut(s) 212, 1390, 1815
TaqII GACCGA 1 cut(s) 655
TatI WGTACW 5 cut(s) 1222, 1429, 2547, 2655, 3390
TauI GCSGC 5 cut(s) 55, 369, 437, 1538, 2317
TscAI CASTG 9 cut(s) 319, 725, 977, 1339, 2160, 2347, 2526, 2709, 2988
TseFI GTSAC 5 cut(s) 310, 412, 1330, 2521, 2570
Tsp45I GTSAC 5 cut(s) 310, 412, 1330, 2521, 2570
TspGWI ACGGA 3 cut(s) 127, 548, 1428
TspRI CASTG 9 cut(s) 319, 725, 977, 1339, 2160, 2347, 2526, 2709, 2988
Tth111I GACNNNGTC 2 cut(s) 296, 1328
Van91I CCANNNNNTGG 1 cut(s) 105
Vha464I CTTAAG 1 cut(s) 3271
VpaK11BI GGWCC 8 cut(s) 90, 424, 638, 752, 2011, 2699, 2972, 3002
XagI CCTNNNNNAGG 1 cut(s) 3274
XapI RAATTY 9 cut(s) 993, 1165, 1205, 1416, 1505, 1626, 2367, 2713, 2977
XceI RCATGY 3 cut(s) 127, 1597, 1694
XhoI CTCGAG 1 cut(s) 1462
XmiI GTMKAC 1 cut(s) 1846
XmnI GAANNNNTTC 1 cut(s) 983
XspI CTAG 6 cut(s) 431, 693, 1400, 2178, 2543, 3332
ZraI GACGTC 1 cut(s) 210
Zsp2I ATGCAT 2 cut(s) 1969, 2223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.