Rw5G048080

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000379
Physical Location & Seq
Reverse (-)
83424486 .. 83428475
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G048080.1

Sequence Viewer

Length: 3519 bp
ATGGTCTGCGTCCCGACGGCGAATGGGGTGGTCGAATTGGGCTCGACGGAGCTGATATTCCAGAGCTCGGATGTGATGAACGAGGCCCGAGTATTGTTTGATTTCAATAATCTTGAAGTCGGCTCTTGGCCTGTAGCTCTGGCCACTTCAGGACCGAGTAGCAGTAACCATCACATCTCTAAGCCGATTCAGTTTGACAACCACCCTAGTTCCAGTAGCTCTTCTGCATCATCAGCTACTCAGTGGAAGTATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGAAGGAGTATTATATCTGATTTATACGATCGGCTGCAAAACAAGAGAGGAATTAGAACATTTATGGATGATCCAGATCTTCGAGTAGGGGATGCTATTTCTCCCGCTCTCTTAAAGGCGATTGAAGAATCAAGGTTTGCAATCGTTGTTCTATCGAAAAATTATGCTCATTCTACTTGGTGTTTGGAGGAACTTACAAGTATCTGTCAGTGCATGAAAGACTTCAACAGAATTCTTCCACTTTTCTATAATGTGGAACCCTCTGATGTAAGGTACCGGAAAAGGAGTTTCCGAAGAGCTTTCGATAAGCATGAACGCTCTGGGCGACACACATCAGAGAAGGTGCAGCGGTGGAATGATGCGTTAAACAAAGTGGCCAGTTTTTCTGGGTGGCATACAAAGAATTTTAAGAGTGATAGAGATCTTGTTGACTCCATTGTGGAATTTGTGTGCAGTAAAGTACAACCTATTGAAATCGGGTTTACAATGTTCGCCGGAGATTTTCAAGCATTTGAAGCAACAAGAAAAAGCATGAATGAGGTTATGAAGGCGCTTCAAGATGACGAGGTCACTGCCCTTGGGGTCTATGGAATGGGAGGGGTCGGCAAGACAACCATGGTTAAACATGTCGGTGCGCTAGCCCGCAAGAATGGAATTTTTAACCATGTACTTCTGGCTGTCATATCCCAAAGCCCTGACTCGAGAAATATTCAAGCCACATTGGCAGAGCTGCTGGGCTTCAAATTCAAGTGGGAGACAGAAATTGGAAGAGCCGCTGAGTTGCATGAGAAGATAATGAGTAAAAATAAGATCCTTATAATCCTGGACGACATGTGGGAGAGAATGGATTTGTCAAGAATAGGAATTCCCAGCTATGACGAGCTCAAAAAATACAATTCTAAAGTCCTACTCACCACGAGGAGATTGCATGTTTGTAATTCCATGGACAGCCAAGTAAGCATTCCTCTCGATATCTTATCAGAAGAAGATTCTTGGAAATTGTTTATGAGAAATGCAGGAAGGTCTTTTGAATCCTCCAATTTTAATAACGTAGCAAGAAAGGTAGCTGCAGAATGTAGGGGTCTACCAATTGCATTGATAGCAGTTGCAAGGGCACTCAGAGATAAAGATCTGGCGGAATGGCAAAAAGCAGCTCAACGACTAGAGAAGTCGCAAATTGCCAACCCTGATGATAAGGGAGATGCATTCGAATGTATAAAATTAAGCTTTGATTACTTGAAAGATGAGGACCACAAGTCATGCTTCTTGCTCTGTTGCCTATTCCCAGAAGACCATGAAATCAATATAGAAGACTTGTTCAGGTATGCGATTGGAAAAGGATTGTTTCAAGATGCCGAAACAATGTACGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGAGAGTGAATACATTGGATGTGTAAAGATGCATGATGTCATCCGGGATACAGCCATGAATATTGCAAAATCTAAAGATGGACATCGGTTTACGGTAAAAGTTGGCTGTGGTTTAGAGGATTGGCGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGAGGAACAAAATTCGCAAGCTACCCGAAGAAGAGTTGGTATGTCCAAATCTCCAGCTTTTATTACTAAAACAGAATGCTGATTTAAATGAGATCCCAGAAAAGTTTATCCAAAATCTGAATAAATTAAGGCTCTTGGATCTTAGCAACACTAGTATTTCCGTATTACCCCAATCATTCAGTCTCCTTACCAACCTTCAAGCTTTGAATTTAGATTTTTGCGAGCAATTGATTGACATATCCGTAGTGGGAAAACTTAAGAAGCTTGAAATTCTTAGTATGAGACAATGTCCTCTCAAGAAACTGTCGAGAGAAATAGGACATTTGACCAATCTAAGAATTTTGGATGTCAGTTCCACTGAATTTCTCATAATTCCACCTAAAGTGATACCGAAGTTGCATAAATTAGAAGAACTGTATATACTAAACTGTGGATTTGAGGATTGGGGGAGTGAAGTTGAGAGAGAAGGAGAAGAAACTGAATTGGTTTTCCCTGTTTTGTCAAATTTGAAAAATTGGCAGGTTTGCATATCTGATGCTAAATGCACACCTAAATGTCTTGAGGTCAATGGAGAATATCATAATTCAAGATCCTTGTTTCTTAATGGAGCAACCATAAGTACCTTACCTGACTGGTTTATCAACACAGTGACAAAGAAAACAGAGAAGCTAGAGTACAGTCGCTGCAAAGGGATGAGTGACATTCTTATGGAACATGACCATGGGAGGTTATATAAGCTCAAGCATCTTAGAGTATGTGGTTATTTTTGTGATTCCTATGTGTACTTGAAGGAGTTGATGAACACAACAAGACGAGTTCAAAAAGGACCAGTGTTTATGAATTTGGAAGAGTTGCATCTGATAGATCTGATCCACCTTAATGAGTTGTGTGTTGGAGAGTTACCACATGGGTCTCTCTTCAATCTGAAGGTATTGCATATATCTTTTTGTAATATTTTGAAGAATGTATCAAAACTGGTACAGAGACTACCAAATCTGGAGAGACTATATTTAAATTGCATGGATGACCTGGAATATGTGTTTGGATGTGAAGGGTTTGAGCCGAAAGAATCAAAACTGAGAGAGATGCAGTTGTTGGGTCCAAATTCAGTGAAGAGTATTTGTAGTGGTCCTGCCCCACGCGTAATGTTCCAGAGTCTTAAAAGTTTGACTTTTTACCGTTGCGAGTTGCTGCAAAGTCTGTTCGCATATGATGTAGCCCAGAATCTTGTTCAATTGGAAGACCTTTTAGTAGAATGGTGCCCTTTGTTGGTAAGAGTAATTGAAGCAGTGAACAACGAGAAGACTACGGTTCTTCGAAAAATGAAGAACTTGGTTTTCATGGGACTTCCCAAGTTGTATGGTGCAAGTGCTACTATTGACATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGGATTGTCCCCGGTTTTCATTTTCAACCTCTTCCTTAAGCAGGAACCCGTCTTCATTTTCAACCTCTGCTTCCGACTACTTTGGCAGCACCAACCTAGTCAAACTCAATGATCAACAACATCTTCAACTTCTATCATCAAGTTTGAAACGAAATTTCAAAGAGGATCATTACAAGTGGAAATGCATGTGCTCTACATATGAAGCAGAAATTTGTGACCTATGA

Protein Analysis

1172

Amino Acids

133.45

Weight (kDa)

6.52

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 83 - 250 2.2e-46 TIR domain
TIR_2 PF13676 86 - 183 2.8e-16 TIR domain
NB-ARC PF00931 271 - 435 9.9e-29 NB-ARC domain
LRR_8 PF13855 642 - 700 2.4e-07 Leucine rich repeat
LRR_14 PF23598 644 - 839 5.2e-13 Leucine-rich repeat region
LRR_RPS2 PF23247 908 - 1047 4e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1103
Acc36I ACCTGC 1 cut(s) 2389
Acc65I GGTACC 1 cut(s) 558
AccB1I GGYRCC 2 cut(s) 558, 3138
AccBSI CCGCTC 1 cut(s) 392
AccI GTMKAC 1 cut(s) 1369
AccII CGCG 2 cut(s) 1845, 3021
AciI CCGC 7 cut(s) 390, 634, 928, 1059, 1421, 1837, 1845
AclWI GGATC 7 cut(s) 350, 1090, 1966, 2025, 2463, 2743, 3468
AcoI YGGCCR 3 cut(s) 141, 660, 1838
AcuI CTGAAG 2 cut(s) 132, 2825
AfaI GTAC 9 cut(s) 560, 747, 954, 1652, 1688, 2500, 2555, 2663, 2859
AfiI CCNNNNNNNGG 3 cut(s) 67, 3148, 3336
AflII CTTAAG 2 cut(s) 2135, 3331
AflIII ACRYGT 3 cut(s) 910, 1116, 3019
AhdI GACNNNNNGTC 1 cut(s) 1540
AhlI ACTAGT 1 cut(s) 2030
AjnI CCWGG 2 cut(s) 1107, 2907
AjuI GAANNNNNNNTTGG 4 cut(s) 1230, 1262, 2904, 2936
Alw21I GWGCWC 3 cut(s) 68, 1170, 3488
Alw26I GTCTC 6 cut(s) 1034, 2066, 2155, 2796, 2857, 2875
AlwI GGATC 7 cut(s) 350, 1090, 1966, 2025, 2463, 2743, 3468
AlwNI CAGNNNCTG 1 cut(s) 2562
Ama87I CYCGRG 2 cut(s) 87, 985
AoxI GGCC 5 cut(s) 84, 128, 141, 660, 1838
ApeKI GCWGC 9 cut(s) 319, 631, 1015, 1352, 1436, 1860, 2562, 3070, 3381
Asp700I GAANNNNTTC 1 cut(s) 506
Asp718I GGTACC 1 cut(s) 558
AspLEI GCGC 2 cut(s) 838, 922
AspS9I GGNCC 7 cut(s) 85, 152, 275, 1534, 2705, 2978, 3008
AsuC2I CCSGG 2 cut(s) 1757, 3307
AsuHPI GGTGA 1 cut(s) 1189
AsuII TTCGAA 2 cut(s) 1494, 3196
AsuNHI GCTAGC 1 cut(s) 922
AvaI CYCGRG 2 cut(s) 87, 985
AvaII GGWCC 6 cut(s) 152, 275, 1534, 2705, 2978, 3008
BaeGI GKGCMC 2 cut(s) 1402, 3143
BaeI ACNNNNGTAYC 2 cut(s) 2258, 2291
BalI TGGCCA 2 cut(s) 143, 662
BanI GGYRCC 2 cut(s) 558, 3138
BanII GRGCYC 3 cut(s) 44, 68, 1170
BarI GAAGNNNNNNTAC 2 cut(s) 2537, 2569
BauI CACGAG 1 cut(s) 1201
BbsI GAAGAC 5 cut(s) 1581, 1602, 3126, 3188, 3339
Bbv12I GWGCWC 3 cut(s) 68, 1170, 3488
BbvI GCAGC 9 cut(s) 306, 643, 1002, 1339, 1448, 1847, 2549, 3057, 3393
BccI CCATC 2 cut(s) 177, 1784
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 6 cut(s) 839, 873, 1192, 1226, 1234, 1268
BciT130I CCWGG 2 cut(s) 1109, 2909
BciVI GTATCC 1 cut(s) 1753
BclI TGATCA 1 cut(s) 3406
BcnI CCSGG 2 cut(s) 1757, 3307
BcoDI GTCTC 6 cut(s) 1034, 2066, 2155, 2796, 2857, 2875
BcuI ACTAGT 1 cut(s) 2030
BfaI CTAG 7 cut(s) 207, 923, 1448, 1701, 2031, 2549, 3392
BfmI CTRYAG 2 cut(s) 132, 1353
BfoI RGCGCY 1 cut(s) 839
BfrI CTTAAG 2 cut(s) 2135, 3331
BfuAI ACCTGC 1 cut(s) 2389
BfuI GTATCC 1 cut(s) 1753
BglI GCCNNNNNGGC 1 cut(s) 1007
BglII AGATCT 4 cut(s) 361, 706, 1414, 2743
Bme1390I CCNGG 4 cut(s) 1109, 1757, 2909, 3307
Bme18I GGWCC 6 cut(s) 152, 275, 1534, 2705, 2978, 3008
BmeRI GACNNNNNGTC 1 cut(s) 1540
BmeT110I CYCGRG 2 cut(s) 87, 985
BmgT120I GGNCC 7 cut(s) 85, 152, 275, 1534, 2705, 2978, 3008
BmiI GGNNCC 6 cut(s) 276, 543, 560, 2979, 3140, 3341
BmrFI CCNGG 4 cut(s) 1109, 1757, 2909, 3307
BmtI GCTAGC 1 cut(s) 926
BpiI GAAGAC 5 cut(s) 1581, 1602, 3126, 3188, 3339
BpmI CTGGAG 2 cut(s) 1916, 2897
Bpu14I TTCGAA 2 cut(s) 1494, 3196
BpuEI CTTGAG 3 cut(s) 2159, 2459, 2603
BpuMI CCSGG 2 cut(s) 1757, 3307
BsaBI GATNNNNATC 4 cut(s) 360, 705, 1413, 1794
BsaI GGTCTC 1 cut(s) 2796
BsaJI CCNNGG 5 cut(s) 862, 900, 1227, 2599, 3305
BsaWI WCCGGW 1 cut(s) 561
Bsc4I CCNNNNNNNGG 3 cut(s) 67, 3148, 3336
Bse1I ACTGG 5 cut(s) 213, 663, 2516, 2708, 2859
Bse8I GATNNNNATC 4 cut(s) 360, 705, 1413, 1794
BseBI CCWGG 2 cut(s) 1109, 2909
BseDI CCNNGG 5 cut(s) 862, 900, 1227, 2599, 3305
BseGI GGATG 9 cut(s) 76, 358, 382, 1736, 1752, 2230, 2577, 2908, 2930
BseJI GATNNNNATC 4 cut(s) 360, 705, 1413, 1794
BseLI CCNNNNNNNGG 3 cut(s) 67, 3148, 3336
BseMII CTCAG 4 cut(s) 254, 1053, 1417, 2948
BseNI ACTGG 5 cut(s) 213, 663, 2516, 2708, 2859
BseRI GAGGAG 1 cut(s) 1219
BseSI GKGCMC 2 cut(s) 1402, 3143
BseXI GCAGC 9 cut(s) 306, 643, 1002, 1339, 1448, 1847, 2549, 3057, 3393
BseYI CCCAGC 2 cut(s) 1018, 1154
BsgI GTGCAG 3 cut(s) 650, 757, 1846
Bsh1236I CGCG 2 cut(s) 1845, 3021
Bsh1285I CGRYCG 1 cut(s) 316
BshFI GGCC 5 cut(s) 86, 130, 143, 662, 1840
BshNI GGYRCC 2 cut(s) 558, 3138
BsiEI CGRYCG 1 cut(s) 316
BsiHKAI GWGCWC 3 cut(s) 68, 1170, 3488
BsiHKCI CYCGRG 2 cut(s) 87, 985
BsiSI CCGG 4 cut(s) 562, 780, 1756, 3307
BslFI GGGAC 2 cut(s) 3237, 3288
BslI CCNNNNNNNGG 3 cut(s) 67, 3148, 3336
BsmAI GTCTC 6 cut(s) 1034, 2066, 2155, 2796, 2857, 2875
BsmFI GGGAC 2 cut(s) 3237, 3288
BsmI GAATGC 3 cut(s) 1245, 1490, 1960
BsnI GGCC 5 cut(s) 86, 130, 143, 662, 1840
Bso31I GGTCTC 1 cut(s) 2796
BsoBI CYCGRG 2 cut(s) 87, 985
Bsp119I TTCGAA 2 cut(s) 1494, 3196
Bsp1286I GDGCHC 6 cut(s) 44, 68, 1170, 1402, 3143, 3488
Bsp19I CCATGG 3 cut(s) 900, 1227, 2599
BspACI CCGC 7 cut(s) 390, 634, 928, 1059, 1421, 1837, 1845
BspANI GGCC 5 cut(s) 86, 130, 143, 662, 1840
BspCNI CTCAG 4 cut(s) 253, 1054, 1416, 2949
BspFNI CGCG 2 cut(s) 1845, 3021
BspLI GGNNCC 6 cut(s) 276, 543, 560, 2979, 3140, 3341
BspMAI CTGCAG 1 cut(s) 1357
BspMI ACCTGC 1 cut(s) 2389
BspOI GCTAGC 1 cut(s) 926
BspPI GGATC 7 cut(s) 350, 1090, 1966, 2025, 2463, 2743, 3468
BspQI GCTCTTC 3 cut(s) 226, 574, 1048
BspT104I TTCGAA 2 cut(s) 1494, 3196
BspT107I GGYRCC 2 cut(s) 558, 3138
BspTI CTTAAG 2 cut(s) 2135, 3331
BspTNI GGTCTC 1 cut(s) 2796
BsrBI CCGCTC 1 cut(s) 392
BsrI ACTGG 5 cut(s) 213, 663, 2516, 2708, 2859
BssECI CCNNGG 5 cut(s) 862, 900, 1227, 2599, 3305
BssSI CACGAG 1 cut(s) 1201
BssT1I CCWWGG 4 cut(s) 862, 900, 1227, 2599
Bst2BI CACGAG 1 cut(s) 1201
Bst2UI CCWGG 2 cut(s) 1109, 2909
Bst4CI ACNGT 8 cut(s) 1807, 2184, 2295, 2309, 2527, 2558, 3059, 3190
Bst6I CTCTTC 8 cut(s) 226, 574, 1048, 1905, 2721, 2801, 2987, 3331
BstAFI CTTAAG 2 cut(s) 2135, 3331
BstBI TTCGAA 2 cut(s) 1494, 3196
BstC8I GCNNGC 5 cut(s) 924, 928, 1706, 1898, 2102
BstDEI CTNAG 9 cut(s) 180, 240, 1062, 1403, 2021, 2153, 2213, 2627, 2957
BstDSI CCRYGG 3 cut(s) 900, 1227, 2599
BstENI CCTNNNNNAGG 1 cut(s) 3334
BstF5I GGATG 9 cut(s) 76, 358, 382, 1736, 1752, 2230, 2577, 2908, 2930
BstFNI CGCG 2 cut(s) 1845, 3021
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 2 cut(s) 838, 922
BstMAI GTCTC 6 cut(s) 1034, 2066, 2155, 2796, 2857, 2875
BstMCI CGRYCG 1 cut(s) 316
BstMWI GCNNNNNNNGC 5 cut(s) 233, 800, 1007, 1242, 1385
BstNI CCWGG 2 cut(s) 1109, 2909
BstNSI RCATGY 4 cut(s) 914, 1120, 1217, 3484
BstSCI CCNGG 4 cut(s) 1107, 1755, 2907, 3305
BstSFI CTRYAG 2 cut(s) 132, 1353
BstSLI GKGCMC 2 cut(s) 1402, 3143
BstUI CGCG 2 cut(s) 1845, 3021
BstV1I GCAGC 9 cut(s) 306, 643, 1002, 1339, 1448, 1847, 2549, 3057, 3393
BstV2I GAAGAC 5 cut(s) 1581, 1602, 3126, 3188, 3339
BstX2I RGATCY 8 cut(s) 361, 706, 1095, 1414, 1971, 2017, 2468, 2743
BstYI RGATCY 8 cut(s) 361, 706, 1095, 1414, 1971, 2017, 2468, 2743
BsuI GTATCC 1 cut(s) 1753
BsuRI GGCC 5 cut(s) 86, 130, 143, 662, 1840
BtgI CCRYGG 3 cut(s) 900, 1227, 2599
BtsCI GGATG 9 cut(s) 76, 358, 382, 1736, 1752, 2230, 2577, 2908, 2930
BtsI GCAGTG 3 cut(s) 855, 1863, 3174
BveI ACCTGC 1 cut(s) 2389
Cac8I GCNNGC 5 cut(s) 924, 928, 1706, 1898, 2102
CaiI CAGNNNCTG 1 cut(s) 2562
CfoI GCGC 2 cut(s) 838, 922
Cfr13I GGNCC 7 cut(s) 85, 152, 275, 1534, 2705, 2978, 3008
Csp6I GTAC 9 cut(s) 559, 746, 953, 1651, 1687, 2499, 2554, 2662, 2858
CviQI GTAC 9 cut(s) 559, 746, 953, 1651, 1687, 2499, 2554, 2662, 2858
DdeI CTNAG 9 cut(s) 180, 240, 1062, 1403, 2021, 2153, 2213, 2627, 2957
DraI TTTAAA 2 cut(s) 1965, 2892
DriI GACNNNNNGTC 1 cut(s) 1540
EaeI YGGCCR 3 cut(s) 141, 660, 1838
Eam1104I CTCTTC 8 cut(s) 226, 574, 1048, 1905, 2721, 2801, 2987, 3331
Eam1105I GACNNNNNGTC 1 cut(s) 1540
EarI CTCTTC 8 cut(s) 226, 574, 1048, 1905, 2721, 2801, 2987, 3331
EciI GGCGGA 1 cut(s) 1436
Ecl136II GAGCTC 2 cut(s) 66, 1168
Eco130I CCWWGG 4 cut(s) 862, 900, 1227, 2599
Eco24I GRGCYC 3 cut(s) 44, 68, 1170
Eco31I GGTCTC 1 cut(s) 2796
Eco32I GATATC 1 cut(s) 1258
Eco47I GGWCC 6 cut(s) 152, 275, 1534, 2705, 2978, 3008
Eco53kI GAGCTC 2 cut(s) 66, 1168
Eco57I CTGAAG 2 cut(s) 132, 2825
Eco88I CYCGRG 2 cut(s) 87, 985
EcoICRI GAGCTC 2 cut(s) 66, 1168
EcoNI CCTNNNNNAGG 1 cut(s) 3334
EcoO109I RGGNCCY 1 cut(s) 275
EcoRI GAATTC 2 cut(s) 516, 1149
EcoRII CCWGG 2 cut(s) 1107, 2907
EcoRV GATATC 1 cut(s) 1258
EcoT14I CCWWGG 4 cut(s) 862, 900, 1227, 2599
EcoT22I ATGCAT 3 cut(s) 1492, 1746, 3482
EcoT38I GRGCYC 3 cut(s) 44, 68, 1170
ErhI CCWWGG 4 cut(s) 862, 900, 1227, 2599
FalI AAGNNNNNCTT 4 cut(s) 1532, 1564, 3034, 3066
FaqI GGGAC 2 cut(s) 3237, 3288
FauI CCCGC 2 cut(s) 397, 935
FauNDI CATATG 2 cut(s) 3088, 3493
FbaI TGATCA 1 cut(s) 3406
FblI GTMKAC 1 cut(s) 1369
FokI GGATG 9 cut(s) 83, 365, 389, 1739, 1743, 2237, 2584, 2915, 2937
FriOI GRGCYC 3 cut(s) 44, 68, 1170
FspBI CTAG 7 cut(s) 207, 923, 1448, 1701, 2031, 2549, 3392
GlaI GCGC 2 cut(s) 837, 921
GsaI CCCAGC 2 cut(s) 1022, 1158
GsuI CTGGAG 2 cut(s) 1916, 2897
HaeII RGCGCY 1 cut(s) 839
HaeIII GGCC 5 cut(s) 86, 130, 143, 662, 1840
HapII CCGG 4 cut(s) 562, 780, 1756, 3307
HhaI GCGC 2 cut(s) 838, 922
Hin6I GCGC 2 cut(s) 836, 920
HinP1I GCGC 2 cut(s) 836, 920
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HindIII AAGCTT 3 cut(s) 1510, 2079, 2141
HpaII CCGG 4 cut(s) 562, 780, 1756, 3307
HphI GGTGA 1 cut(s) 1189
Hpy166II GTNNAC 6 cut(s) 715, 768, 1370, 1803, 2662, 3172
Hpy8I GTNNAC 6 cut(s) 715, 768, 1370, 1803, 2662, 3172
Hpy99I CGWCG 2 cut(s) 19, 49
HpyCH4III ACNGT 8 cut(s) 1807, 2184, 2295, 2309, 2527, 2558, 3059, 3190
HpyCH4IV ACGT 1 cut(s) 1335
HpyF10VI GCNNNNNNNGC 5 cut(s) 233, 800, 1007, 1242, 1385
HpyF3I CTNAG 9 cut(s) 180, 240, 1062, 1403, 2021, 2153, 2213, 2627, 2957
HpySE526I ACGT 1 cut(s) 1335
HspAI GCGC 2 cut(s) 836, 920
KpnI GGTACC 1 cut(s) 562
Ksp22I TGATCA 1 cut(s) 3406
LguI GCTCTTC 3 cut(s) 226, 574, 1048
LmnI GCTCC 2 cut(s) 49, 2486
Lsp1109I GCAGC 9 cut(s) 306, 643, 1002, 1339, 1448, 1847, 2549, 3057, 3393
MaeI CTAG 7 cut(s) 207, 923, 1448, 1701, 2031, 2549, 3392
MaeII ACGT 1 cut(s) 1335
MaeIII GTNAC 6 cut(s) 164, 853, 2527, 2576, 2778, 3509
MbiI CCGCTC 1 cut(s) 392
MfeI CAATTG 3 cut(s) 1374, 2105, 3113
MflI RGATCY 8 cut(s) 361, 706, 1095, 1414, 1971, 2017, 2468, 2743
MhlI GDGCHC 6 cut(s) 44, 68, 1170, 1402, 3143, 3488
MlsI TGGCCA 2 cut(s) 143, 662
MluI ACGCGT 1 cut(s) 3019
MluNI TGGCCA 2 cut(s) 143, 662
MlyI GAGTC 3 cut(s) 710, 977, 3043
MmeI TCCRAC 2 cut(s) 2752, 3393
Mox20I TGGCCA 2 cut(s) 143, 662
Mph1103I ATGCAT 3 cut(s) 1492, 1746, 3482
MroXI GAANNNNTTC 1 cut(s) 506
MscI TGGCCA 2 cut(s) 143, 662
MslI CAYNNNNRTG 6 cut(s) 915, 1495, 2431, 2585, 2598, 2757
Msp20I TGGCCA 2 cut(s) 143, 662
MspA1I CMGCKG 2 cut(s) 634, 1061
MspCI CTTAAG 2 cut(s) 2135, 3331
MspI CCGG 4 cut(s) 562, 780, 1756, 3307
MspR9I CCNGG 4 cut(s) 1109, 1757, 2909, 3307
MunI CAATTG 3 cut(s) 1374, 2105, 3113
Mva1269I GAATGC 3 cut(s) 1245, 1490, 1960
MvaI CCWGG 2 cut(s) 1109, 2909
MvnI CGCG 2 cut(s) 1845, 3021
MwoI GCNNNNNNNGC 5 cut(s) 233, 800, 1007, 1242, 1385
NciI CCSGG 2 cut(s) 1757, 3307
NcoI CCATGG 3 cut(s) 900, 1227, 2599
NdeI CATATG 2 cut(s) 3088, 3493
NheI GCTAGC 1 cut(s) 922
NlaIV GGNNCC 6 cut(s) 276, 543, 560, 2979, 3140, 3341
NmuCI GTSAC 4 cut(s) 853, 2527, 2576, 3509
NsiI ATGCAT 3 cut(s) 1492, 1746, 3482
NspI RCATGY 4 cut(s) 914, 1120, 1217, 3484
NspV TTCGAA 2 cut(s) 1494, 3196
PaeR7I CTCGAG 1 cut(s) 985
PciI ACATGT 2 cut(s) 910, 1116
PciSI GCTCTTC 3 cut(s) 226, 574, 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 607
PctI GAATGC 3 cut(s) 1245, 1490, 1960
PdmI GAANNNNTTC 1 cut(s) 506
PfeI GAWTC 9 cut(s) 187, 413, 1274, 1316, 1673, 1697, 2651, 2948, 3103
PflFI GACNNNGTC 2 cut(s) 851, 2166
PfoI TCCNGGA 2 cut(s) 1107, 1755
Ple19I CGATCG 1 cut(s) 316
PleI GAGTC 3 cut(s) 710, 977, 3042
PpsI GAGTC 3 cut(s) 710, 977, 3042
PpuMI RGGWCCY 1 cut(s) 275
PscI ACATGT 2 cut(s) 910, 1116
PsiI TTATAA 1 cut(s) 1103
Psp124BI GAGCTC 2 cut(s) 68, 1170
Psp5II RGGWCCY 1 cut(s) 275
Psp6I CCWGG 2 cut(s) 1107, 2907
PspFI CCCAGC 2 cut(s) 1018, 1154
PspGI CCWGG 2 cut(s) 1107, 2907
PspN4I GGNNCC 6 cut(s) 276, 543, 560, 2979, 3140, 3341
PspPI GGNCC 7 cut(s) 85, 152, 275, 1534, 2705, 2978, 3008
PspPPI RGGWCCY 1 cut(s) 275
PsrI GAACNNNNNNTAC 2 cut(s) 2283, 2315
PstI CTGCAG 1 cut(s) 1357
PstNI CAGNNNCTG 1 cut(s) 2562
PsuI RGATCY 8 cut(s) 361, 706, 1095, 1414, 1971, 2017, 2468, 2743
PsyI GACNNNGTC 2 cut(s) 851, 2166
PvuI CGATCG 1 cut(s) 316
RsaI GTAC 9 cut(s) 560, 747, 954, 1652, 1688, 2500, 2555, 2663, 2859
RsaNI GTAC 9 cut(s) 559, 746, 953, 1651, 1687, 2499, 2554, 2662, 2858
RseI CAYNNNNRTG 6 cut(s) 915, 1495, 2431, 2585, 2598, 2757
SacI GAGCTC 2 cut(s) 68, 1170
SapI GCTCTTC 3 cut(s) 226, 574, 1048
Sau96I GGNCC 7 cut(s) 85, 152, 275, 1534, 2705, 2978, 3008
SchI GAGTC 3 cut(s) 710, 977, 3043
ScrFI CCNGG 4 cut(s) 1109, 1757, 2909, 3307
SduI GDGCHC 6 cut(s) 44, 68, 1170, 1402, 3143, 3488
SfcI CTRYAG 2 cut(s) 132, 1353
Sfr274I CTCGAG 1 cut(s) 985
SfuI TTCGAA 2 cut(s) 1494, 3196
SinI GGWCC 6 cut(s) 152, 275, 1534, 2705, 2978, 3008
SlaI CTCGAG 1 cut(s) 985
SmiI ATTTAAAT 2 cut(s) 1965, 2892
SmiMI CAYNNNNRTG 6 cut(s) 915, 1495, 2431, 2585, 2598, 2757
SmlI CTYRAG 6 cut(s) 985, 2135, 2174, 2438, 2618, 3331
SmoI CTYRAG 6 cut(s) 985, 2135, 2174, 2438, 2618, 3331
SpeI ACTAGT 1 cut(s) 2030
SsiI CCGC 7 cut(s) 390, 634, 928, 1059, 1421, 1837, 1845
SspI AATATT 3 cut(s) 994, 1774, 2833
SspMI CTAG 7 cut(s) 207, 923, 1448, 1701, 2031, 2549, 3392
SstI GAGCTC 2 cut(s) 68, 1170
StyD4I CCNGG 4 cut(s) 1107, 1755, 2907, 3305
StyI CCWWGG 4 cut(s) 862, 900, 1227, 2599
SwaI ATTTAAAT 2 cut(s) 1965, 2892
TaaI ACNGT 8 cut(s) 1807, 2184, 2295, 2309, 2527, 2558, 3059, 3190
TaiI ACGT 1 cut(s) 1338
TaqII GACCGA 1 cut(s) 169
TatI WGTACW 4 cut(s) 745, 952, 2553, 2661
TauI GCSGC 2 cut(s) 1061, 1840
TfiI GAWTC 9 cut(s) 187, 413, 1274, 1316, 1673, 1697, 2651, 2948, 3103
TseFI GTSAC 4 cut(s) 853, 2527, 2576, 3509
TseI GCWGC 9 cut(s) 319, 631, 1015, 1352, 1436, 1860, 2562, 3070, 3381
Tsp45I GTSAC 4 cut(s) 853, 2527, 2576, 3509
TspGWI ACGGA 3 cut(s) 62, 2029, 2110
Tth111I GACNNNGTC 2 cut(s) 851, 2166
Vha464I CTTAAG 2 cut(s) 2135, 3331
VpaK11BI GGWCC 6 cut(s) 152, 275, 1534, 2705, 2978, 3008
XagI CCTNNNNNAGG 1 cut(s) 3334
XceI RCATGY 4 cut(s) 914, 1120, 1217, 3484
XhoI CTCGAG 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 1369
XmnI GAANNNNTTC 1 cut(s) 506
XspI CTAG 7 cut(s) 207, 923, 1448, 1701, 2031, 2549, 3392
Zsp2I ATGCAT 3 cut(s) 1492, 1746, 3482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.