FvH4_3g43991

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000379
Physical Location & Seq
Reverse (-)
36679710 .. 36682628
2919 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43991.t1

Sequence Viewer

Length: 2796 bp
ATGTCCACCGGAGATTTAGAAGCGTATGAAGCAACAAGAAAATCCATGGGTGAGATTATGAAGAAGCTTAAAGATGACAACGTCACTGCCCTTGCGGTCTACGGAATGGGCGGAGTCGGCAAGACAACCCTGGTAAAACATGTCGCTGCTCAATCCTGCAAAAATGGGATTTTTCATCATGTGGTTATGGCTGTTGTATCCCAAAGCCCCAACGTTGAAAGAATTCAACACACATTACTAGAAGAGCTGGTCGGCTTTGAGAATTTAACAGAAAGTGGAAGAGCCTCTAGATTGTATAAAGAGATAATGAGGAAACACAATATCCTCATACTCTTGGACGACATTTGGGGGTTTATAGATTTGTCCAGAATAGGAATTCCCAGCTATGACCTGCTCAAAAAATGCAATTCTAAAGTCCTAATCACCACGAGAAAATTGCATGTTTGTAATTCCATGCACAGCCAAGCAAGCATTCTTCTCAATATCTTATCAGAACAAGATTCTTGGAGCATGTTTGTGAAGAAAACAAAAAGATCTTTTGAATCAACCACTTTTGAGGGTGTAGCGAGAAAGGTTGCTGGAGAATGTAGGGGTCTACCCATTGCTCTGATAGCAGTTGCAAGGGCACTTGGAGATAAAGATCTGGCGGAATGGCAAACAGCAGCTCAACAATTAAAGAAGTCGCAAATGGCCAACCCTGATCATGATGACACAGCTTTCGAATGTTTGAAATTAAGCTATGATTACTTGAAACATGAGGACTACAAATCATGCTTCTTGTTGTGTTGCCTATTTCCAGAAGACTATGACATCCCAATAGAAGACTTGTTCAAGTATGCAATTGGGAAAGGATTGTTTCGAGACACCGACACAATATACGAAGCCAGAGCAATAGCTGACACAGTGGTCAAGCACCTGATAGATTCTAGCTTGCTTTTGGATGGTAAATATGATGGACATGTAAGGATGCACGATGTTGTCCGGGATACAGCCATGAGAATTGCAAAATGTGAAGATGGCCATGGGTTTTTGGTGAGAGCTGGATGTGGTCTTAAGGATTGGTCATGTCGATCACATGAAGACTACTATGCTATCTCACTAATGGGGAATGAACTTCACAGTCTACCCGAAGAGTTGATAGGTTCAAAACTTAAGATTTTATTACTACAAGGGAATCGGTATTTAAATGAGATCCCAGAGACCTTTTCCCAAAGTCGAAATGAATTAAGAGTCTTAGATCTTAGCCGCACCAACATTTCATTGCTGCCCCAATCATTCAGTCTCCTGACCAAGCTCCAAGCTCTGCATCTAGATTTTTGCTTGAAAGCAGTTGACATTTCCATAATCGGAAAACTTAAGAAGCTTGAGATTCTTAGTATGAGACAATATCCTCTTACGGAGCTGTCTAGAGAAATAGGAAATTTGACCAATCTAAGGATGTTGGATATCGATGGCTGGAGTTCTGAAGGTGGAGGTATTGTCACAATTCCATCTGAAGTGATATCAAAGCTGCATAGAATAGAAGAATTGTACATGATGAACTGTGGATTTGTGAACTGGGAAAGTCCAATTGAGGGAGAAGGAGATGAAATCAATATTGGCTTTGGTGAGCTAGCTGGTTTATCAAAGTTGAAAATTTTGCAGGTTTTCTTACCTAATGCAGAATACATCCCTAAAAATGTCGAGGTCGAACCAGGTTGGTTTTACTTTGATATACATATTGGGTGCACAAGTGGTCTAGATTATCAGTTTGATCGTAAATCAAGATCCTTGCTTCTTCGTAAAATAACCATCAGTACCTTACCTGATTGGTTTATCAAAGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATGACTGCTGCGGAGGGATGAGTGACATTGTTATGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCACTGTTGCTGGGCATCGTCTTCATTCTTATGTGTACTTGAAAGAGTTGATGAACACAATAAGACGAGTTGAAACAAGACCAGTGTTTGAGAATTTGGAAGAGTTGCATCTGATAGATCTGATCCACCTGGAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCAAATCTCAAGGTATTGCATATGAAATGTTGTTTTATCTTGAAGAGTGTATCAAGATTTGTACAGAGACTACCAAATCTGGAGAAACTATATCTGAATTGGATGAAGGAAATGGAACATGTGTTTGGATGCGAAGGGTGTGAGCCAGAACAATCAAAACTGAGAGAGATGTATTTGTTGTCTCTAGAAGCACTAAGAAGCATATGTAATGGTCCGGCTCCACGTGCAATGTTCCGGAGTCTTAAAACCTTGACCGTGTACGATTGCGAGCTGCTGCAAAGTCTGTTCGCATCTGATGTAGCTGAGTGTCTTGTTCAATTGGAAGACCTTTTTGTAGAGGGGTGTCCTTTGTTGGAAAGAGTAACGGAAGCAGTGAACAAAGATAAGACGGTTCTACCAAACCTGAAGAACTTGGTTTTGATGAATCTTCCTATGTTGTATGGTCCAAGTGCTACTACTGTTGATATTGAGTGTCCTTCATTGGAACACCTGGTGGTGGTGGATTGCCCCCAATTTTCATTTTCAATCTCTTCCGATCTCTTTGAAGACTTGGAAAGCCGGAACCAGTTTTCATTTTCAACCTCCGCTTCTGACTACTTTGGCAGCAGGAACCCAGTCCAACTCAGTGATCCACAACTGTACGAGTTTTTAGGTCGCAGGAAGTCTAAGTGGATGGGGGAGCATATGAAATGGTTCTGCTGA

Protein Analysis

932

Amino Acids

105.83

Weight (kDa)

5.82

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 14 - 176 4.8e-30 NB-ARC domain
LRR_14 PF23598 383 - 516 2.1e-14 Leucine-rich repeat region
LRR_8 PF13855 383 - 440 1.4e-07 Leucine rich repeat
LRR_RPS2 PF23247 685 - 813 6.8e-18 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 905
Acc36I ACCTGC 2 cut(s) 399, 1633
AccI GTMKAC 3 cut(s) 99, 595, 1123
AccIII TCCGGA 1 cut(s) 2328
AciI CCGC 7 cut(s) 95, 111, 647, 1246, 1823, 1863, 2679
AclI AACGTT 1 cut(s) 213
AclWI GGATC 4 cut(s) 1186, 1761, 2041, 2717
AcoI YGGCCR 2 cut(s) 690, 1018
AcsI RAATTY 6 cut(s) 222, 262, 375, 1420, 1635, 2017
AcuI CTGAAG 3 cut(s) 1485, 1515, 2519
AcvI CACGTG 1 cut(s) 2318
AdeI CACNNNGTG 1 cut(s) 2587
AfaI GTAC 6 cut(s) 1532, 1798, 1961, 2157, 2354, 2735
AfiI CCNNNNNNNGG 4 cut(s) 1434, 1574, 2056, 2590
AflII CTTAAG 3 cut(s) 1052, 1151, 1355
AflIII ACRYGT 3 cut(s) 139, 958, 2212
AjnI CCWGG 5 cut(s) 129, 1693, 2052, 2083, 2583
AjuI GAANNNNNNNTTGG 6 cut(s) 456, 488, 1581, 1613, 2202, 2234
Alw21I GWGCWC 1 cut(s) 1730
Alw26I GTCTC 7 cut(s) 855, 1193, 1286, 1375, 2094, 2155, 2280
Alw44I GTGCAC 1 cut(s) 1726
AlwI GGATC 4 cut(s) 1186, 1761, 2041, 2717
Aor13HI TCCGGA 1 cut(s) 2328
AoxI GGCC 2 cut(s) 690, 1018
ApaLI GTGCAC 1 cut(s) 1726
ApeKI GCWGC 8 cut(s) 146, 662, 1264, 1510, 1860, 2365, 2368, 2697
ApoI RAATTY 6 cut(s) 222, 262, 375, 1420, 1635, 2017
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
Asp700I GAANNNNTTC 2 cut(s) 222, 2786
AspS9I GGNCC 2 cut(s) 2306, 2537
AsuC2I CCSGG 1 cut(s) 983
AsuHPI GGTGA 6 cut(s) 62, 415, 1045, 1619, 1837, 2084
AsuII TTCGAA 1 cut(s) 720
AsuNHI GCTAGC 1 cut(s) 1612
AvaII GGWCC 2 cut(s) 2306, 2537
BaeGI GKGCMC 2 cut(s) 628, 1730
BalI TGGCCA 2 cut(s) 692, 1020
BauI CACGAG 1 cut(s) 427
BbrPI CACGTG 1 cut(s) 2318
BbsI GAAGAC 6 cut(s) 807, 828, 1086, 1937, 2424, 2646
Bbv12I GWGCWC 1 cut(s) 1730
BbvI GCAGC 8 cut(s) 133, 674, 1251, 1497, 1847, 2352, 2355, 2709
BccI CCATC 7 cut(s) 935, 947, 1010, 1445, 1498, 1799, 2761
BcgI CGANNNNNNTGC 2 cut(s) 418, 452
BciT130I CCWGG 5 cut(s) 131, 1695, 2054, 2085, 2585
BciVI GTATCC 2 cut(s) 208, 979
BclI TGATCA 1 cut(s) 700
BcnI CCSGG 1 cut(s) 983
BcoDI GTCTC 7 cut(s) 855, 1193, 1286, 1375, 2094, 2155, 2280
BfaI CTAG 9 cut(s) 239, 288, 927, 1310, 1407, 1613, 1739, 1847, 2279
BfrI CTTAAG 3 cut(s) 1052, 1151, 1355
BfuAI ACCTGC 2 cut(s) 399, 1633
BfuI GTATCC 2 cut(s) 208, 979
BglII AGATCT 4 cut(s) 533, 640, 1237, 2041
BisI GCNGC 9 cut(s) 147, 663, 1246, 1265, 1511, 1861, 2366, 2369, 2698
BlsI GCNGC 9 cut(s) 148, 664, 1247, 1266, 1512, 1862, 2367, 2370, 2699
Bme1390I CCNGG 6 cut(s) 131, 983, 1695, 2054, 2085, 2585
Bme18I GGWCC 2 cut(s) 2306, 2537
BmgT120I GGNCC 2 cut(s) 2306, 2537
BmiI GGNNCC 3 cut(s) 2313, 2657, 2705
BmrFI CCNGG 6 cut(s) 131, 983, 1695, 2054, 2085, 2585
BmrI ACTGGG 2 cut(s) 1567, 2702
BmsI GCATC 7 cut(s) 957, 1315, 1930, 1948, 2041, 2213, 2393
BmtI GCTAGC 1 cut(s) 1616
BmuI ACTGGG 2 cut(s) 1567, 2702
BpiI GAAGAC 6 cut(s) 807, 828, 1086, 1937, 2424, 2646
BpmI CTGGAG 4 cut(s) 600, 1477, 2075, 2195
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 3 cut(s) 1385, 1901, 2087
BpuMI CCSGG 1 cut(s) 983
Bsa29I ATCGAT 1 cut(s) 1449
BsaAI YACGTR 1 cut(s) 2318
BsaBI GATNNNNATC 1 cut(s) 639
BsaI GGTCTC 2 cut(s) 1193, 2094
BsaJI CCNNGG 5 cut(s) 45, 129, 1021, 1897, 2084
BsaWI WCCGGW 2 cut(s) 8, 2328
BsaXI ACNNNNNCTCC 4 cut(s) 1464, 1494, 2048, 2078
Bsc4I CCNNNNNNNGG 4 cut(s) 1434, 1574, 2056, 2590
Bse1I ACTGG 4 cut(s) 1562, 2006, 2659, 2708
Bse3DI GCAATG 3 cut(s) 600, 1259, 2328
Bse8I GATNNNNATC 1 cut(s) 639
BseAI TCCGGA 1 cut(s) 2328
BseBI CCWGG 5 cut(s) 131, 1695, 2054, 2085, 2585
BseCI ATCGAT 1 cut(s) 1449
BseDI CCNNGG 5 cut(s) 45, 129, 1021, 1897, 2084
BseJI GATNNNNATC 1 cut(s) 639
BseLI CCNNNNNNNGG 4 cut(s) 1434, 1574, 2056, 2590
BseMI GCAATG 3 cut(s) 600, 1259, 2328
BseMII CTCAG 3 cut(s) 2246, 2388, 2731
BseNI ACTGG 4 cut(s) 1562, 2006, 2659, 2708
BseRI GAGGAG 1 cut(s) 2072
BseSI GKGCMC 2 cut(s) 628, 1730
BseXI GCAGC 8 cut(s) 133, 674, 1251, 1497, 1847, 2352, 2355, 2709
BseYI CCCAGC 2 cut(s) 380, 1934
BshFI GGCC 2 cut(s) 692, 1020
BshVI ATCGAT 1 cut(s) 1449
BsiHKAI GWGCWC 1 cut(s) 1730
BsiSI CCGG 5 cut(s) 9, 982, 2309, 2329, 2653
BslI CCNNNNNNNGG 4 cut(s) 1434, 1574, 2056, 2590
BsmAI GTCTC 7 cut(s) 855, 1193, 1286, 1375, 2094, 2155, 2280
BsmI GAATGC 1 cut(s) 471
BsnI GGCC 2 cut(s) 692, 1020
Bso31I GGTCTC 2 cut(s) 1193, 2094
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 2 cut(s) 628, 1730
Bsp13I TCCGGA 1 cut(s) 2328
Bsp1407I TGTACA 2 cut(s) 1530, 2155
Bsp19I CCATGG 3 cut(s) 45, 1021, 1897
BspACI CCGC 7 cut(s) 95, 111, 647, 1246, 1823, 1863, 2679
BspANI GGCC 2 cut(s) 692, 1020
BspCNI CTCAG 3 cut(s) 2247, 2389, 2730
BspDI ATCGAT 1 cut(s) 1449
BspEI TCCGGA 1 cut(s) 2328
BspHI TCATGA 1 cut(s) 703
BspLI GGNNCC 3 cut(s) 2313, 2657, 2705
BspMI ACCTGC 2 cut(s) 399, 1633
BspOI GCTAGC 1 cut(s) 1616
BspPI GGATC 4 cut(s) 1186, 1761, 2041, 2717
BspQI GCTCTTC 2 cut(s) 237, 274
BspT104I TTCGAA 1 cut(s) 720
BspTI CTTAAG 3 cut(s) 1052, 1151, 1355
BspTNI GGTCTC 2 cut(s) 1193, 2094
BsrDI GCAATG 3 cut(s) 600, 1259, 2328
BsrGI TGTACA 2 cut(s) 1530, 2155
BsrI ACTGG 4 cut(s) 1562, 2006, 2659, 2708
BssECI CCNNGG 5 cut(s) 45, 129, 1021, 1897, 2084
BssSI CACGAG 1 cut(s) 427
BssT1I CCWWGG 3 cut(s) 45, 1021, 1897
Bst2BI CACGAG 1 cut(s) 427
Bst2UI CCWGG 5 cut(s) 131, 1695, 2054, 2085, 2585
Bst4CI ACNGT 8 cut(s) 904, 1121, 1544, 1930, 2350, 2485, 2554, 2733
Bst6I CTCTTC 6 cut(s) 237, 274, 1125, 2019, 2132, 2629
BstAFI CTTAAG 3 cut(s) 1052, 1151, 1355
BstAUI TGTACA 2 cut(s) 1530, 2155
BstBAI YACGTR 1 cut(s) 2318
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 4 cut(s) 469, 932, 1614, 2363
BstDEI CTNAG 9 cut(s) 1234, 1241, 1373, 1433, 2255, 2288, 2397, 2717, 2760
BstDSI CCRYGG 3 cut(s) 45, 1021, 1897
BstMAI GTCTC 7 cut(s) 855, 1193, 1286, 1375, 2094, 2155, 2280
BstMWI GCNNNNNNNGC 5 cut(s) 29, 117, 468, 611, 2318
BstNI CCWGG 5 cut(s) 131, 1695, 2054, 2085, 2585
BstNSI RCATGY 5 cut(s) 143, 443, 514, 962, 2216
BstSCI CCNGG 6 cut(s) 129, 981, 1693, 2052, 2083, 2583
BstSLI GKGCMC 2 cut(s) 628, 1730
BstV1I GCAGC 8 cut(s) 133, 674, 1251, 1497, 1847, 2352, 2355, 2709
BstV2I GAAGAC 6 cut(s) 807, 828, 1086, 1937, 2424, 2646
BstX2I RGATCY 6 cut(s) 533, 640, 1191, 1237, 1766, 2041
BstYI RGATCY 6 cut(s) 533, 640, 1191, 1237, 1766, 2041
Bsu15I ATCGAT 1 cut(s) 1449
BsuI GTATCC 2 cut(s) 208, 979
BsuRI GGCC 2 cut(s) 692, 1020
BsuTUI ATCGAT 1 cut(s) 1449
BtgI CCRYGG 3 cut(s) 45, 1021, 1897
BtsI GCAGTG 2 cut(s) 84, 2472
BtsIMutI CAGTG 6 cut(s) 84, 909, 1926, 2013, 2472, 2725
BveI ACCTGC 2 cut(s) 399, 1633
Cac8I GCNNGC 4 cut(s) 469, 932, 1614, 2363
CciI TCATGA 1 cut(s) 703
Cfr13I GGNCC 2 cut(s) 2306, 2537
ClaI ATCGAT 1 cut(s) 1449
CsiI ACCWGGT 2 cut(s) 1693, 2583
Csp6I GTAC 6 cut(s) 1531, 1797, 1960, 2156, 2353, 2734
CviQI GTAC 6 cut(s) 1531, 1797, 1960, 2156, 2353, 2734
DdeI CTNAG 9 cut(s) 1234, 1241, 1373, 1433, 2255, 2288, 2397, 2717, 2760
DraI TTTAAA 1 cut(s) 1185
DraIII CACNNNGTG 1 cut(s) 2587
DrdI GACNNNNNNGTC 1 cut(s) 905
DseDI GACNNNNNNGTC 1 cut(s) 905
EaeI YGGCCR 2 cut(s) 690, 1018
Eam1104I CTCTTC 6 cut(s) 237, 274, 1125, 2019, 2132, 2629
EarI CTCTTC 6 cut(s) 237, 274, 1125, 2019, 2132, 2629
EciI GGCGGA 2 cut(s) 126, 662
Eco130I CCWWGG 3 cut(s) 45, 1021, 1897
Eco31I GGTCTC 2 cut(s) 1193, 2094
Eco32I GATATC 2 cut(s) 1447, 1503
Eco47I GGWCC 2 cut(s) 2306, 2537
Eco57I CTGAAG 3 cut(s) 1485, 1515, 2519
Eco72I CACGTG 1 cut(s) 2318
EcoRI GAATTC 2 cut(s) 222, 375
EcoRII CCWGG 5 cut(s) 129, 1693, 2052, 2083, 2583
EcoRV GATATC 2 cut(s) 1447, 1503
EcoT14I CCWWGG 3 cut(s) 45, 1021, 1897
ErhI CCWWGG 3 cut(s) 45, 1021, 1897
FauNDI CATATG 3 cut(s) 2115, 2297, 2778
FbaI TGATCA 1 cut(s) 700
FblI GTMKAC 3 cut(s) 99, 595, 1123
Fnu4HI GCNGC 9 cut(s) 147, 663, 1246, 1265, 1511, 1861, 2366, 2369, 2698
Fsp4HI GCNGC 9 cut(s) 147, 663, 1246, 1265, 1511, 1861, 2366, 2369, 2698
FspBI CTAG 9 cut(s) 239, 288, 927, 1310, 1407, 1613, 1739, 1847, 2279
GluI GCNGC 9 cut(s) 147, 663, 1246, 1265, 1511, 1861, 2366, 2369, 2698
GsaI CCCAGC 2 cut(s) 384, 1938
GsuI CTGGAG 4 cut(s) 600, 1477, 2075, 2195
HaeIII GGCC 2 cut(s) 692, 1020
HapII CCGG 5 cut(s) 9, 982, 2309, 2329, 2653
HincII GTYRAC 1 cut(s) 1333
HindII GTYRAC 1 cut(s) 1333
HindIII AAGCTT 2 cut(s) 65, 1361
HinfI GANTC 9 cut(s) 114, 500, 542, 923, 1174, 1230, 1369, 2332, 2518
HpaII CCGG 5 cut(s) 9, 982, 2309, 2329, 2653
HphI GGTGA 6 cut(s) 62, 415, 1045, 1619, 1837, 2084
HpyAV CCTTC 5 cut(s) 1460, 1574, 2194, 2222, 2580
HpyCH4III ACNGT 8 cut(s) 904, 1121, 1544, 1930, 2350, 2485, 2554, 2733
HpyCH4IV ACGT 3 cut(s) 81, 213, 2317
HpyF10VI GCNNNNNNNGC 5 cut(s) 29, 117, 468, 611, 2318
HpyF3I CTNAG 9 cut(s) 1234, 1241, 1373, 1433, 2255, 2288, 2397, 2717, 2760
HpySE526I ACGT 3 cut(s) 81, 213, 2317
Kpn2I TCCGGA 1 cut(s) 2328
Ksp22I TGATCA 1 cut(s) 700
LguI GCTCTTC 2 cut(s) 237, 274
LmnI GCTCC 5 cut(s) 507, 1299, 1399, 2317, 2773
Lsp1109I GCAGC 8 cut(s) 133, 674, 1251, 1497, 1847, 2352, 2355, 2709
LweI GCATC 7 cut(s) 957, 1315, 1930, 1948, 2041, 2213, 2393
MabI ACCWGGT 2 cut(s) 1693, 2583
MaeI CTAG 9 cut(s) 239, 288, 927, 1310, 1407, 1613, 1739, 1847, 2279
MaeII ACGT 3 cut(s) 81, 213, 2317
MaeIII GTNAC 7 cut(s) 82, 1480, 1825, 1874, 1905, 2076, 2455
MfeI CAATTG 3 cut(s) 840, 1569, 2411
MflI RGATCY 6 cut(s) 533, 640, 1191, 1237, 1766, 2041
MhlI GDGCHC 2 cut(s) 628, 1730
MlsI TGGCCA 2 cut(s) 692, 1020
MluNI TGGCCA 2 cut(s) 692, 1020
MlyI GAGTC 3 cut(s) 123, 1239, 2341
MmeI TCCRAC 3 cut(s) 1422, 2427, 2737
Mox20I TGGCCA 2 cut(s) 692, 1020
MroI TCCGGA 1 cut(s) 2328
MroXI GAANNNNTTC 2 cut(s) 222, 2786
MscI TGGCCA 2 cut(s) 692, 1020
MslI CAYNNNNRTG 3 cut(s) 515, 1883, 1953
Msp20I TGGCCA 2 cut(s) 692, 1020
MspCI CTTAAG 3 cut(s) 1052, 1151, 1355
MspI CCGG 5 cut(s) 9, 982, 2309, 2329, 2653
MspR9I CCNGG 6 cut(s) 131, 983, 1695, 2054, 2085, 2585
MunI CAATTG 3 cut(s) 840, 1569, 2411
Mva1269I GAATGC 1 cut(s) 471
MvaI CCWGG 5 cut(s) 131, 1695, 2054, 2085, 2585
MwoI GCNNNNNNNGC 5 cut(s) 29, 117, 468, 611, 2318
NciI CCSGG 1 cut(s) 983
NcoI CCATGG 3 cut(s) 45, 1021, 1897
NdeI CATATG 3 cut(s) 2115, 2297, 2778
NheI GCTAGC 1 cut(s) 1612
NlaIV GGNNCC 3 cut(s) 2313, 2657, 2705
NmuCI GTSAC 4 cut(s) 82, 1480, 1825, 1874
NspI RCATGY 5 cut(s) 143, 443, 514, 962, 2216
NspV TTCGAA 1 cut(s) 720
PagI TCATGA 1 cut(s) 703
PciI ACATGT 3 cut(s) 139, 958, 2212
PciSI GCTCTTC 2 cut(s) 237, 274
PctI GAATGC 1 cut(s) 471
PdmI GAANNNNTTC 2 cut(s) 222, 2786
PfeI GAWTC 6 cut(s) 500, 542, 923, 1174, 1369, 2518
PflFI GACNNNGTC 1 cut(s) 80
PfoI TCCNGGA 1 cut(s) 981
PkrI GCNGC 9 cut(s) 148, 664, 1247, 1266, 1512, 1862, 2367, 2370, 2699
PleI GAGTC 3 cut(s) 122, 1238, 2340
PmaCI CACGTG 1 cut(s) 2318
PmlI CACGTG 1 cut(s) 2318
PpsI GAGTC 3 cut(s) 122, 1238, 2340
Ppu21I YACGTR 1 cut(s) 2318
PscI ACATGT 3 cut(s) 139, 958, 2212
Psp1406I AACGTT 1 cut(s) 213
Psp6I CCWGG 5 cut(s) 129, 1693, 2052, 2083, 2583
PspCI CACGTG 1 cut(s) 2318
PspFI CCCAGC 2 cut(s) 380, 1934
PspGI CCWGG 5 cut(s) 129, 1693, 2052, 2083, 2583
PspN4I GGNNCC 3 cut(s) 2313, 2657, 2705
PspPI GGNCC 2 cut(s) 2306, 2537
PsuI RGATCY 6 cut(s) 533, 640, 1191, 1237, 1766, 2041
PsyI GACNNNGTC 1 cut(s) 80
RsaI GTAC 6 cut(s) 1532, 1798, 1961, 2157, 2354, 2735
RsaNI GTAC 6 cut(s) 1531, 1797, 1960, 2156, 2353, 2734
RseI CAYNNNNRTG 3 cut(s) 515, 1883, 1953
SapI GCTCTTC 2 cut(s) 237, 274
SatI GCNGC 9 cut(s) 147, 663, 1246, 1265, 1511, 1861, 2366, 2369, 2698
Sau96I GGNCC 2 cut(s) 2306, 2537
SchI GAGTC 3 cut(s) 123, 1239, 2341
ScrFI CCNGG 6 cut(s) 131, 983, 1695, 2054, 2085, 2585
SduI GDGCHC 2 cut(s) 628, 1730
SexAI ACCWGGT 2 cut(s) 1693, 2583
SfaNI GCATC 7 cut(s) 957, 1315, 1930, 1948, 2041, 2213, 2393
SfuI TTCGAA 1 cut(s) 720
SinI GGWCC 2 cut(s) 2306, 2537
SmiI ATTTAAAT 1 cut(s) 1185
SmiMI CAYNNNNRTG 3 cut(s) 515, 1883, 1953
SmlI CTYRAG 6 cut(s) 1052, 1151, 1355, 1364, 1916, 2102
SmoI CTYRAG 6 cut(s) 1052, 1151, 1355, 1364, 1916, 2102
SsiI CCGC 7 cut(s) 95, 111, 647, 1246, 1823, 1863, 2679
SspI AATATT 1 cut(s) 1597
SspMI CTAG 9 cut(s) 239, 288, 927, 1310, 1407, 1613, 1739, 1847, 2279
StyD4I CCNGG 6 cut(s) 129, 981, 1693, 2052, 2083, 2583
StyI CCWWGG 3 cut(s) 45, 1021, 1897
SwaI ATTTAAAT 1 cut(s) 1185
TaaI ACNGT 8 cut(s) 904, 1121, 1544, 1930, 2350, 2485, 2554, 2733
TaiI ACGT 3 cut(s) 84, 216, 2320
TaqI TCGA 7 cut(s) 720, 859, 1069, 1216, 1449, 1683, 1689
TatI WGTACW 3 cut(s) 1530, 1959, 2155
TauI GCSGC 1 cut(s) 1248
TfiI GAWTC 6 cut(s) 500, 542, 923, 1174, 1369, 2518
TscAI CASTG 6 cut(s) 91, 909, 1933, 2013, 2472, 2725
TseFI GTSAC 4 cut(s) 82, 1480, 1825, 1874
TseI GCWGC 8 cut(s) 146, 662, 1264, 1510, 1860, 2365, 2368, 2697
Tsp45I GTSAC 4 cut(s) 82, 1480, 1825, 1874
TspGWI ACGGA 3 cut(s) 117, 1412, 2474
TspRI CASTG 6 cut(s) 91, 909, 1933, 2013, 2472, 2725
Tth111I GACNNNGTC 1 cut(s) 80
Vha464I CTTAAG 3 cut(s) 1052, 1151, 1355
VneI GTGCAC 1 cut(s) 1726
VpaK11BI GGWCC 2 cut(s) 2306, 2537
XapI RAATTY 6 cut(s) 222, 262, 375, 1420, 1635, 2017
XbaI TCTAGA 5 cut(s) 287, 1309, 1406, 1738, 2278
XceI RCATGY 5 cut(s) 143, 443, 514, 962, 2216
XmiI GTMKAC 3 cut(s) 99, 595, 1123
XmnI GAANNNNTTC 2 cut(s) 222, 2786
XspI CTAG 9 cut(s) 239, 288, 927, 1310, 1407, 1613, 1739, 1847, 2279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.