Rroxscaffold_1G00003250

Disease resistance protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000379
Physical Location & Seq
Forward (+)
3808899 .. 3816477
7579 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00003250.1

Sequence Viewer

Length: 1875 bp
ATGCATGATGTCATCCGGGATACAGCCCTGAATATTGCAAAATCTAAAGATGGGCACAGGTTTCTGGTAAAAGCTGGGTGTGGTTTAAAGGATTGGCCACGCGGATTACATGAAGGCTGCACTGCTATTTCACTAATGATGAATGAAATCCGCAATCTACACGTAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAAGCGGAATGCTGATTTAAATGAGATCCCAGAAAAGTTTATCCAAAGTCTGAATGAATTAAGGGTATTGGATCTTAGCAACACCAGTATTTCCTTACTACCCCAATCATTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTTTTGCGACAAATTGATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGGGGGAATATCCTGGGGAGGAATTGTCGAGAGAAATAGGACATTTGACCAATCTAACGATTTTGGATGTCAGTGGTGCAAGGGATAGAGCTTTAGTCAGAATTCCATCTAAAGTGATATCAAAGTTTCATAAATTAGAAGAATTGTACATGCTGAACTGTGGATTTGAGGACTGGGGGTGTGAAGTTGAGGGACAAGGAGGAGAAACTAATATTGGGTTTGATGAGTTAGCAGGTTTATTAAATTTGAAAATTTTGCAGGTTTGCATATCTGATGCAAAATATATCCCTAAAAATGTTGAGGCCGAATCGAATTGGGTTTACTTTGTTATATGTATCTGCAGATCCACAGGAGGAGATTTATACAATCGAGAAGATCATAATTCAAGATCCTTGGTTCTTAATGGAGCAACCATAAGTACCTTACCTGATTGGTTTATCAATGCGGTGACAATGAAAACGGAGAAGCTAGGGTATTATTGCTGCCAAGGGATGAGTGACATTCTTATAGAATATGACCATGGGAGGTTACATGAACTCAAGCATCTCAGAGTATGCGGAGATCATCTTAGTTCCCATGTGTACTTGACAGAGTTGATGAATACAACAAGACGAGTTCAAAATGGACCAGTGTTTGAGAACTTGGAAGAGTTGCATCTGATAGATCTGATCCACCTTAAGGAGTTGTGTGTTGGTGAGTTACCACCTGGGTCTCTCTCGAATCTCAAGGTATTGCATATGTTGTTTTGTATAATCTTGAAGAATGTATCAAAATTGGTACAAAGACTCCTAAATCTGGAGAAACTACATTTAAATCATATGGATGAATTGGAATATGTGCTTGGATGCGAAGGGTTTGAGCCGAAACAATCAAAACTGAGAGAGATGCATTTGTTGGTTCCAAATTCTGTGAAAAGCATTTGTAGTGTTCCTGTCCCACGTGTAATGTTTCAGAGTCTTAAAAGTTTGACCATTTATCGTTGCGAGCTGTTGCAAAGTCTGTTCACATCTGATGTAGCTGAATGTCTTGTTCAATTGGAAGACCTTTTTGTAGAGCGGTGCCCTTTGTTGAAAAGAGTAATTGAAGCAGTGAACAACGAGAAGACAGTTCTTCCAGAATTGAAGAACTTGGTTTTGAAGGAACTTCCTATGTTGTATGGTGCAAGTGCTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATTGTGGTGAACTGCCCCCAATTTTCATTTTCAACCTCTTCCAATCTCTTCCGCGCCTTCAAAAGCAGGAACCAGATTTCATTTTCAACCTCTGCTTCTGACTACTTTTGCAGCAGGAACCCAGTCAAACTCAATGATCGACAGCTGCATGACTTCCTATATGATAGCTCAACATATTTCTCATTTGGATATATATTTTTCCCAATTAGATGCAACCGTGTATCTGCTTTCCAAGACTTTCCTCTTTCAATCTTCTGA

Protein Analysis

624

Amino Acids

70.95

Weight (kDa)

5.89

Isoelectric Point (pI)

38.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 60 - 118 2e-09 Leucine rich repeat
LRR_14 PF23598 62 - 191 3.8e-14 Leucine-rich repeat region
LRR_RPS2 PF23247 363 - 491 9e-14 Plant disease resistance protein RPS2-like, leucine-rich repeats
LRR_RPS2 PF23247 433 - 547 2.6e-11 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000379)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata RLG00000002871
rosa_multiflora Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 635, 661
AccB1I GGYRCC 1 cut(s) 1470
AccBSI CCGCTC 1 cut(s) 1468
AccII CGCG 2 cut(s) 102, 1671
AciI CCGC 7 cut(s) 102, 151, 205, 857, 969, 1468, 1669
AclWI GGATC 5 cut(s) 220, 279, 750, 795, 1075
AcoI YGGCCR 1 cut(s) 95
AcsI RAATTY 5 cut(s) 402, 513, 655, 663, 1315
AcuI CTGAAG 1 cut(s) 410
AcvI CACGTG 1 cut(s) 1352
AfaI GTAC 4 cut(s) 560, 832, 995, 1191
AfiI CCNNNNNNNGG 2 cut(s) 1090, 1207
AflII CTTAAG 1 cut(s) 1088
AflIII ACRYGT 2 cut(s) 160, 1351
AjnI CCWGG 2 cut(s) 424, 1117
AjuI GAANNNNNNNTTGG 4 cut(s) 609, 641, 1236, 1268
AluBI AGCT 9 cut(s) 74, 335, 397, 503, 880, 1399, 1430, 1762, 1785
AluI AGCT 9 cut(s) 74, 335, 397, 503, 880, 1399, 1430, 1762, 1785
Alw26I GTCTC 2 cut(s) 320, 1128
AlwI GGATC 5 cut(s) 220, 279, 750, 795, 1075
AoxI GGCC 2 cut(s) 95, 714
ApeKI GCWGC 4 cut(s) 117, 894, 1728, 1762
ApoI RAATTY 5 cut(s) 402, 513, 655, 663, 1315
AspLEI GCGC 1 cut(s) 1673
AspS9I GGNCC 1 cut(s) 1037
AsuC2I CCSGG 1 cut(s) 17
AsuHPI GGTGA 3 cut(s) 871, 1118, 1636
AvaII GGWCC 1 cut(s) 1037
BaeGI GKGCMC 2 cut(s) 57, 1475
BalI TGGCCA 1 cut(s) 97
BanI GGYRCC 1 cut(s) 1470
BbrPI CACGTG 1 cut(s) 1352
BbsI GAAGAC 2 cut(s) 1458, 1520
BbvI GCAGC 4 cut(s) 104, 881, 1740, 1749
BccI CCATC 2 cut(s) 44, 526
BciT130I CCWGG 2 cut(s) 426, 1119
BciVI GTATCC 1 cut(s) 13
BcnI CCSGG 1 cut(s) 17
BcoDI GTCTC 2 cut(s) 320, 1128
BfaI CTAG 1 cut(s) 881
BfmI CTRYAG 2 cut(s) 375, 751
BfrI CTTAAG 1 cut(s) 1088
BfuAI ACCTGC 2 cut(s) 635, 661
BfuI GTATCC 1 cut(s) 13
BglII AGATCT 1 cut(s) 1075
BisI GCNGC 4 cut(s) 118, 895, 1729, 1763
BlsI GCNGC 4 cut(s) 119, 896, 1730, 1764
Bme1390I CCNGG 3 cut(s) 17, 426, 1119
Bme18I GGWCC 1 cut(s) 1037
BmgT120I GGNCC 1 cut(s) 1037
BmiI GGNNCC 4 cut(s) 1311, 1472, 1688, 1736
BmrFI CCNGG 3 cut(s) 17, 426, 1119
BmrI ACTGGG 2 cut(s) 595, 1733
BmsI GCATC 6 cut(s) 676, 964, 1075, 1247, 1287, 1817
BmuI ACTGGG 2 cut(s) 595, 1733
BpiI GAAGAC 2 cut(s) 1458, 1520
BpmI CTGGAG 2 cut(s) 170, 1229
BpuEI CTTGAG 2 cut(s) 935, 1121
BpuMI CCSGG 1 cut(s) 17
BsaAI YACGTR 2 cut(s) 163, 1352
BsaI GGTCTC 1 cut(s) 1128
BsaJI CCNNGG 5 cut(s) 425, 804, 898, 931, 1118
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 1182, 1212
Bsc4I CCNNNNNNNGG 2 cut(s) 1090, 1207
Bse1I ACTGG 4 cut(s) 285, 590, 1040, 1739
BseBI CCWGG 2 cut(s) 426, 1119
BseDI CCNNGG 5 cut(s) 425, 804, 898, 931, 1118
BseGI GGATG 5 cut(s) 12, 484, 909, 1240, 1262
BseLI CCNNNNNNNGG 2 cut(s) 1090, 1207
BseMII CTCAG 2 cut(s) 973, 1280
BseNI ACTGG 4 cut(s) 285, 590, 1040, 1739
BseRI GAGGAG 2 cut(s) 627, 780
BseSI GKGCMC 2 cut(s) 57, 1475
BseXI GCAGC 4 cut(s) 104, 881, 1740, 1749
BseYI CCCAGC 1 cut(s) 74
BsgI GTGCAG 1 cut(s) 103
Bsh1236I CGCG 2 cut(s) 102, 1671
BshFI GGCC 2 cut(s) 97, 716
BshNI GGYRCC 1 cut(s) 1470
BsiSI CCGG 1 cut(s) 16
BslFI GGGAC 2 cut(s) 618, 1331
BslI CCNNNNNNNGG 2 cut(s) 1090, 1207
BsmAI GTCTC 2 cut(s) 320, 1128
BsmFI GGGAC 2 cut(s) 618, 1331
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 2 cut(s) 97, 716
Bso31I GGTCTC 1 cut(s) 1128
Bsp1286I GDGCHC 2 cut(s) 57, 1475
Bsp1407I TGTACA 1 cut(s) 558
Bsp143I GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
Bsp19I CCATGG 1 cut(s) 931
BspACI CCGC 7 cut(s) 102, 151, 205, 857, 969, 1468, 1669
BspANI GGCC 2 cut(s) 97, 716
BspCNI CTCAG 2 cut(s) 972, 1281
BspFNI CGCG 2 cut(s) 102, 1671
BspLI GGNNCC 4 cut(s) 1311, 1472, 1688, 1736
BspMAI CTGCAG 1 cut(s) 755
BspMI ACCTGC 2 cut(s) 635, 661
BspPI GGATC 5 cut(s) 220, 279, 750, 795, 1075
BspT107I GGYRCC 1 cut(s) 1470
BspTI CTTAAG 1 cut(s) 1088
BspTNI GGTCTC 1 cut(s) 1128
BsrBI CCGCTC 1 cut(s) 1468
BsrGI TGTACA 1 cut(s) 558
BsrI ACTGG 4 cut(s) 285, 590, 1040, 1739
BssECI CCNNGG 5 cut(s) 425, 804, 898, 931, 1118
BssMI GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
BssT1I CCWWGG 3 cut(s) 804, 898, 931
Bst2UI CCWGG 2 cut(s) 426, 1119
Bst4CI ACNGT 4 cut(s) 572, 1519, 1585, 1835
Bst6I CTCTTC 3 cut(s) 1053, 1660, 1670
BstAFI CTTAAG 1 cut(s) 1088
BstAUI TGTACA 1 cut(s) 558
BstBAI YACGTR 2 cut(s) 163, 1352
BstC8I GCNNGC 1 cut(s) 1397
BstDEI CTNAG 5 cut(s) 275, 407, 959, 980, 1289
BstDSI CCRYGG 1 cut(s) 931
BstF5I GGATG 5 cut(s) 12, 484, 909, 1240, 1262
BstFNI CGCG 2 cut(s) 102, 1671
BstHHI GCGC 1 cut(s) 1673
BstKTI GATC 9 cut(s) 228, 274, 758, 790, 803, 976, 1078, 1083, 1756
BstMAI GTCTC 2 cut(s) 320, 1128
BstMBI GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
BstNI CCWGG 2 cut(s) 426, 1119
BstNSI RCATGY 1 cut(s) 565
BstSCI CCNGG 3 cut(s) 15, 424, 1117
BstSFI CTRYAG 2 cut(s) 375, 751
BstSLI GKGCMC 2 cut(s) 57, 1475
BstUI CGCG 2 cut(s) 102, 1671
BstV1I GCAGC 4 cut(s) 104, 881, 1740, 1749
BstV2I GAAGAC 2 cut(s) 1458, 1520
BstX2I RGATCY 5 cut(s) 225, 271, 755, 800, 1075
BstYI RGATCY 5 cut(s) 225, 271, 755, 800, 1075
BsuI GTATCC 1 cut(s) 13
BsuRI GGCC 2 cut(s) 97, 716
BtgI CCRYGG 1 cut(s) 931
BtsCI GGATG 5 cut(s) 12, 484, 909, 1240, 1262
BtsI GCAGTG 2 cut(s) 120, 1506
BtsIMutI CAGTG 4 cut(s) 120, 490, 1047, 1506
BveI ACCTGC 2 cut(s) 635, 661
Cac8I GCNNGC 1 cut(s) 1397
CfoI GCGC 1 cut(s) 1673
Cfr13I GGNCC 1 cut(s) 1037
Csp6I GTAC 4 cut(s) 559, 831, 994, 1190
CviAII CATG 7 cut(s) 5, 110, 562, 932, 944, 989, 1766
CviQI GTAC 4 cut(s) 559, 831, 994, 1190
DdeI CTNAG 5 cut(s) 275, 407, 959, 980, 1289
DpnI GATC 9 cut(s) 227, 273, 757, 789, 802, 975, 1077, 1082, 1755
DpnII GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
DraI TTTAAA 3 cut(s) 87, 219, 1224
EaeI YGGCCR 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 1053, 1660, 1670
EarI CTCTTC 3 cut(s) 1053, 1660, 1670
Eco130I CCWWGG 3 cut(s) 804, 898, 931
Eco31I GGTCTC 1 cut(s) 1128
Eco32I GATATC 1 cut(s) 531
Eco47I GGWCC 1 cut(s) 1037
Eco57I CTGAAG 1 cut(s) 410
Eco72I CACGTG 1 cut(s) 1352
EcoRI GAATTC 1 cut(s) 513
EcoRII CCWGG 2 cut(s) 424, 1117
EcoRV GATATC 1 cut(s) 531
EcoT14I CCWWGG 3 cut(s) 804, 898, 931
EcoT22I ATGCAT 2 cut(s) 6, 1302
ErhI CCWWGG 3 cut(s) 804, 898, 931
FaeI CATG 7 cut(s) 8, 113, 565, 935, 947, 992, 1769
FaqI GGGAC 2 cut(s) 618, 1331
FatI CATG 7 cut(s) 4, 109, 561, 931, 943, 988, 1765
FauNDI CATATG 2 cut(s) 1149, 1230
Fnu4HI GCNGC 4 cut(s) 118, 895, 1729, 1763
FokI GGATG 4 cut(s) 491, 916, 1247, 1269
Fsp4HI GCNGC 4 cut(s) 118, 895, 1729, 1763
FspBI CTAG 1 cut(s) 881
GlaI GCGC 1 cut(s) 1672
GluI GCNGC 4 cut(s) 118, 895, 1729, 1763
GsaI CCCAGC 1 cut(s) 78
GsuI CTGGAG 2 cut(s) 170, 1229
HaeIII GGCC 2 cut(s) 97, 716
HapII CCGG 1 cut(s) 16
HhaI GCGC 1 cut(s) 1673
Hin1II CATG 7 cut(s) 8, 113, 565, 935, 947, 992, 1769
Hin6I GCGC 1 cut(s) 1671
HinP1I GCGC 1 cut(s) 1671
HindIII AAGCTT 2 cut(s) 333, 395
HinfI GANTC 4 cut(s) 719, 1132, 1197, 1366
HpaII CCGG 1 cut(s) 16
HphI GGTGA 3 cut(s) 871, 1118, 1636
Hpy166II GTNNAC 5 cut(s) 733, 994, 1416, 1504, 1627
Hpy188III TCNNGA 8 cut(s) 187, 441, 782, 798, 1129, 1168, 1208, 1526
Hpy8I GTNNAC 5 cut(s) 733, 994, 1416, 1504, 1627
HpyAV CCTTC 5 cut(s) 107, 1256, 1543, 1611, 1684
HpyCH4III ACNGT 4 cut(s) 572, 1519, 1585, 1835
HpyCH4IV ACGT 2 cut(s) 162, 1351
HpyF3I CTNAG 5 cut(s) 275, 407, 959, 980, 1289
HpySE526I ACGT 2 cut(s) 162, 1351
Hsp92II CATG 7 cut(s) 8, 113, 565, 935, 947, 992, 1769
HspAI GCGC 1 cut(s) 1671
Kzo9I GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
LmnI GCTCC 1 cut(s) 818
Lsp1109I GCAGC 4 cut(s) 104, 881, 1740, 1749
LweI GCATC 6 cut(s) 676, 964, 1075, 1247, 1287, 1817
MaeI CTAG 1 cut(s) 881
MaeII ACGT 2 cut(s) 162, 1351
MaeIII GTNAC 4 cut(s) 859, 908, 939, 1110
MalI GATC 9 cut(s) 227, 273, 757, 789, 802, 975, 1077, 1082, 1755
MbiI CCGCTC 1 cut(s) 1468
MboI GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
MfeI CAATTG 1 cut(s) 1445
MflI RGATCY 5 cut(s) 225, 271, 755, 800, 1075
MhlI GDGCHC 2 cut(s) 57, 1475
MlsI TGGCCA 1 cut(s) 97
MluNI TGGCCA 1 cut(s) 97
MlyI GAGTC 2 cut(s) 1191, 1375
Mox20I TGGCCA 1 cut(s) 97
Mph1103I ATGCAT 2 cut(s) 6, 1302
MscI TGGCCA 1 cut(s) 97
MseI TTAA 8 cut(s) 86, 218, 260, 653, 813, 1089, 1223, 1371
MslI CAYNNNNRTG 1 cut(s) 1233
Msp20I TGGCCA 1 cut(s) 97
MspA1I CMGCKG 1 cut(s) 1762
MspCI CTTAAG 1 cut(s) 1088
MspI CCGG 1 cut(s) 16
MspR9I CCNGG 3 cut(s) 17, 426, 1119
MunI CAATTG 1 cut(s) 1445
Mva1269I GAATGC 1 cut(s) 214
MvaI CCWGG 2 cut(s) 426, 1119
MvnI CGCG 2 cut(s) 102, 1671
NciI CCSGG 1 cut(s) 17
NcoI CCATGG 1 cut(s) 931
NdeI CATATG 2 cut(s) 1149, 1230
NdeII GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
NlaIII CATG 7 cut(s) 8, 113, 565, 935, 947, 992, 1769
NlaIV GGNNCC 4 cut(s) 1311, 1472, 1688, 1736
NmuCI GTSAC 2 cut(s) 859, 908
NsiI ATGCAT 2 cut(s) 6, 1302
NspI RCATGY 1 cut(s) 565
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 719, 1132
PfoI TCCNGGA 1 cut(s) 15
PkrI GCNGC 4 cut(s) 119, 896, 1730, 1764
PleI GAGTC 2 cut(s) 1191, 1374
PmaCI CACGTG 1 cut(s) 1352
PmlI CACGTG 1 cut(s) 1352
PpsI GAGTC 2 cut(s) 1191, 1374
Ppu21I YACGTR 2 cut(s) 163, 1352
Psp6I CCWGG 2 cut(s) 424, 1117
PspCI CACGTG 1 cut(s) 1352
PspFI CCCAGC 1 cut(s) 74
PspGI CCWGG 2 cut(s) 424, 1117
PspN4I GGNNCC 4 cut(s) 1311, 1472, 1688, 1736
PspPI GGNCC 1 cut(s) 1037
PstI CTGCAG 1 cut(s) 755
PsuI RGATCY 5 cut(s) 225, 271, 755, 800, 1075
PvuII CAGCTG 1 cut(s) 1762
RsaI GTAC 4 cut(s) 560, 832, 995, 1191
RsaNI GTAC 4 cut(s) 559, 831, 994, 1190
RseI CAYNNNNRTG 1 cut(s) 1233
SaqAI TTAA 8 cut(s) 86, 218, 260, 653, 813, 1089, 1223, 1371
SatI GCNGC 4 cut(s) 118, 895, 1729, 1763
Sau3AI GATC 9 cut(s) 225, 271, 755, 787, 800, 973, 1075, 1080, 1753
Sau96I GGNCC 1 cut(s) 1037
SchI GAGTC 2 cut(s) 1191, 1375
ScrFI CCNGG 3 cut(s) 17, 426, 1119
SduI GDGCHC 2 cut(s) 57, 1475
SfaNI GCATC 6 cut(s) 676, 964, 1075, 1247, 1287, 1817
SfcI CTRYAG 2 cut(s) 375, 751
SinI GGWCC 1 cut(s) 1037
SmiI ATTTAAAT 2 cut(s) 219, 1224
SmiMI CAYNNNNRTG 1 cut(s) 1233
SmlI CTYRAG 3 cut(s) 950, 1088, 1136
SmoI CTYRAG 3 cut(s) 950, 1088, 1136
SsiI CCGC 7 cut(s) 102, 151, 205, 857, 969, 1468, 1669
SspI AATATT 2 cut(s) 34, 625
SspMI CTAG 1 cut(s) 881
StyD4I CCNGG 3 cut(s) 15, 424, 1117
StyI CCWWGG 3 cut(s) 804, 898, 931
SwaI ATTTAAAT 2 cut(s) 219, 1224
TaaI ACNGT 4 cut(s) 572, 1519, 1585, 1835
TaiI ACGT 2 cut(s) 165, 1354
TaqI TCGA 5 cut(s) 440, 722, 781, 1130, 1756
TatI WGTACW 2 cut(s) 558, 993
TfiI GAWTC 2 cut(s) 719, 1132
Tru1I TTAA 8 cut(s) 86, 218, 260, 653, 813, 1089, 1223, 1371
Tru9I TTAA 8 cut(s) 86, 218, 260, 653, 813, 1089, 1223, 1371
TscAI CASTG 4 cut(s) 127, 490, 1047, 1506
TseFI GTSAC 2 cut(s) 859, 908
TseI GCWGC 4 cut(s) 117, 894, 1728, 1762
Tsp45I GTSAC 2 cut(s) 859, 908
TspGWI ACGGA 1 cut(s) 887
TspRI CASTG 4 cut(s) 127, 490, 1047, 1506
Vha464I CTTAAG 1 cut(s) 1088
VpaK11BI GGWCC 1 cut(s) 1037
XapI RAATTY 5 cut(s) 402, 513, 655, 663, 1315
XceI RCATGY 1 cut(s) 565
XspI CTAG 1 cut(s) 881
Zsp2I ATGCAT 2 cut(s) 6, 1302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.