Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0000379

Physical Location & Seq

Genomic Coordinates Reverse (-)

83650755 .. 83654513

Gene Structure 3759 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G048180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3378 bp

ATGGCCTCCTCTTCATCTGTTCCTTCATCATCATCAGCTCCTCGCTGGACGCATGATGTGTTTTTGAGTTTCAGGGGTCCTGACACTCGCAAGGGTATCACATCTGATCTATACCGTCGACTGCAGTGGAGGGGAATTAAAACATTCATGGATGATCGAGATCTTGAATTTGGGGATGCTATTTCTCCGGCTCTCATAGAAGCAATTCAAGAATCAAGGTTTGCAATTGTTGTTCTCTCGCGAAACTACGCTTCTTCTGCTTGGTGTTTGGAGGAACTTGCAAAGATCTGTGAGTGCATGAAAGATCGGAAGAGGATTTTTCCACTTTTTTTTGAGGTGGAGCCCTATGAGGTAAGATATCAGAAGAGGAGTTTTGATGAAGCTTTCCCTGAGCATGAAAAGCGACACGGATCGGATAAGGTGAAGCAGTGGAGAGCTGCTTTAAACAAAGTGACCGCTTTCCTTGGCTGGGAGACGAAGAAGTATAAGACTCACAAAAGACTTGTTAAACACCTTGTGGAATTTGTCCGCAGTAAAGTACCACTTGATGCAATTGAGCCCGCAGAAGGTTTTGAAGAATATGAAGCAACAATAGAAGCCATGGATGAGGTTAAGAAGGCACTAACAGATGACAAGATCACTGCCGTTGTTGTCTACGGCATGGGCGGCGTTGGAAAGACAAGCATGGTGAGACATGTAGCTGCACAAGCCTGCAAAAATGGTACTTTTAACCATTGGATTATGGCTGCCATATCCCAAAGCCCTGACTACAGAAAAATTCAAGGCACATTAGCAGACCTATTAGGCGTCAAATTGGAGGAAGAGACAGAAATTGGAAGAGCCGCTAGGCTGCTTAATGAGATAAGGAGAAGAGAAAAACTCCTGATAATCGTGGACGATGTTTGGAACAGAACAGAACTGTCAACCATAGGAATTCCCAGCTACAAGGAGCTTCAAAAGTGCAGATCCAAAGTCTTACTCACCACACGGAGAAGGAATGTCTGTGATTCCATGGACAGCCAAGAAAACATTCCTCTCAATATCTTATCAGAACAAGATTCTTGGAAATTGTTTCTGAGAAATGCAGGGGTAGCGAAGAAGAAGATTCTTGAATCGGCCTCTTTTGAGGATGTGGCAAGGAGAGTAGCCGGAGAATGCAGGGGTCTACCCATTGCATTGATAGCAGTTGCAAGGGCACTCAGAGATAAAGATGAGGTGGAATGGGATAAAGCAGCTGGAAGACTAGAGAAGTCGCAATCTGCAACCGTTGAGGATGGGCAAGAGGCCTTCGAATGTATAAAATTGAGCTATGATTTCTTGAAAGACGAGGATTCCAAGTCATGCTTCTTGCTCTGTTGCCTATACCCAGAAGACCATGACATCCGACTAGAAAACTTATTCAAGTATGCAATCGGGACAGGATTGTTTCGACGAGATGCCGAAACAATGGACGAAGCCAGAGGAACAGCAGATTCAGTGGTCAAGTACCTGAAAGATTCTAGCTTGCTTTTGGATAGTGAAGAAGATGGATGTGTAAAGATGCATGACGTCATCCGGGATACAGCCCTGAATATTGCAAAGTCTAAAGATGGGCACGGGTTTCTGGTAAAAGCTGGGTGTGGTTTAAAGGTTTGGCCATGTGGATCACATCAAGGCTACTCTGCAATCTCACTAATGAGGAACGAAATTCGCAAGCTGCCCGATGAAGAGTTGGTATGTCCAAATCTCCAGATTTTATTACTAAATCGGAATGCTAATTTAAATAAGATCCCAGAAAAATTTATCAAAAATCTGAATGAATTAAGGCTCTTGGATCTTAGCAACACTAGAATTTCCGTATTACCCCAATCATTCAGTCTCCTTACCAACCTCCAAGCTTTGTATTTAGATTTTGCCTTGACTTTTTGCGGGAAGTCGATTGACATTTCTATAGTGGGAAAACTGAAGAAGCTTGAAATTCTTAGTATGAGGGAATATCCTGGGGAGGAATTTTCCAGAGAAATAGGACATTTGACCAATCTAAGGATTTTGGATGTCAGTGGCGGTTGGGGTGGAGGTAAAGTCACAATTCCATCTAAAGTGATATCAAAGTTGCATAAATTAGAAGAACTGTATATACTGAGATGTAGATTTGAGGATTGGGGGAGTAAAGTTGAGAGAGAAGGAGAAGAAACCAATATTTTTCATGAGTTAGTTGGTTTATCAAATTTGAAAATATTGCAGGTTGGCATATCTGATGTAAACTACATCCCTAAAAATGTTGAGGCCAAACCAGATTGGGATTACTTTAATATAAGTATCATGTGCAGAGACATCTTCTGTGCATTTAAACAAGAAGGTCATCATCCAAGATTCTTGTTTCTTAGCGAAGCAACCATGAGTATCTTACCTGATTGGTTTATCAACGCGGTGACAGAGAAAACAGAGAAGCTAGAGTATTATTGCTGCAAAGGGATGAGTGACATTCTTGTGGAATATGACCATGGGAGGTTACATAAACTCAAGCATCTCATGGTATGTGGAATACATCTTGATTCCGATGCGTACTTGACAGAGTTGATGAATACAACAAGCCGAGTTCGAAAAGGACCAGTGTTTGAGAACTTGGAGGAGTTGCATCTGACACATCTGAAACACCTTAAGGAGTTGTCTGTTGGTGAGTTACCACACGGGTCTCTCTTCAATCTGAAGGTATTGCATATGACTTTTTGTCTTAAGTTGAAGAATGTATCAAAATTTGTACAGAAACTATCAAATCTAGAGAAACTAGATTTAAATTGTATGTATGAATTGGAATATGTGTTTGGATGGGAAGGGTTTGAGCCGAAACAATCAAAACTGAGAGAGATGCATTTGTTGGGTCTAAATTCAGTGAAGAGCATTTGTAGTGGTCCTGCCCCACGAGTAATGTTTCAGAGTCTTAAAAGCTTGACCATTTACTGTTGCGAGTTGCTGCGAAGTCTGTTCGCATGTGATGTAGCCGAGTGTCTCTTTCAATTGGAAGACCTTTTTGTAGAGCGGTGCCGTTTGTTGGAAAGAGTAATTGAAGCAGTGAACAACGAGAAGACGGTTCTACCAAAATTGAAGAACTTGGTTTTGAAGAATCTTCCTATGTTGTATGGTGCAAGTGGTACTGTTGATATTGAGTGTCCTTCATTGGAACACTTGATTGTGGAGGATTGCCCCCGGTTTTCATTTTCAGATACTTCCGATTACTTTGATTCCATCAAAAGCATGACCCATATATCATTTTCAACCTCTGGTTCTGACTACTTTGGCAGCACGAACCCAGTCCAACTCAATGATTTTCAAATGTACCAATTTCTACGTGACAGACACTCAGAATGTTGTTTTTATGTTAAAATTTATAACATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008150 GO:0009987 GO:0016020 GO:0023052 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

LRR_8 NB-ARC TIR

Protein Analysis

1125

Amino Acids

128.59

Weight (kDa)

6.4

Isoelectric Point (pI)

46.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	18 - 177	3.4e-46	TIR domain
TIR_2	PF13676	20 - 115	4.6e-16	TIR domain
NB-ARC	PF00931	200 - 363	4.1e-28	NB-ARC domain
LRR_8	PF13855	574 - 630	3.1e-08	Leucine rich repeat
LRR_14	PF23598	583 - 713	2.5e-15	Leucine-rich repeat region
LRR_RPS2	PF23247	869 - 1009	3.6e-15	Plant disease resistance protein RPS2-like, leucine-rich repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000379)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_1g17740 FvH4_3g42940 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43860 FvH4_3g43871 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43880 FvH4_3g43940 FvH4_3g43991 FvH4_3g44561 FvH4_3g44570 FvH4_3g44610 FvH4_3g44670 FvH4_3g44670 FvH4_3g44680 FvH4_3g44680 FvH4_3g44680 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45170 FvH4_3g45630 FvH4_3g45643 FvH4_3g45680 FvH4_3g45690 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45700 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45711 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730 FvH4_3g45730
malus_domestica	MD11G1020200.v1.1 MD15G1398500.v1.1
rosa_chinensis	RchiOBHm_Chr1g0326201 RchiOBHm_Chr5g0064091 RchiOBHm_Chr5g0076771 RchiOBHm_Chr5g0078181 RchiOBHm_Chr5g0078321 RchiOBHm_Chr5g0079331 RchiOBHm_Chr5g0079341 RchiOBHm_Chr5g0079351 RchiOBHm_Chr5g0079361 RchiOBHm_Chr5g0079441 RchiOBHm_Chr5g0079821 RchiOBHm_Chr5g0080071 RchiOBHm_Chr7g0212731 RchiOBHm_Chr7g0224201
rosa_laevigata	RLG00000002871
rosa_multiflora	Rmu_co8378879.1_g000001 Rmu_co8466133.1_g000001 Rmu_sc0000595.1_g000019 Rmu_sc0000616.1_g000025 Rmu_sc0000631.1_g000033 Rmu_sc0000945.1_g000004 Rmu_sc0001148.1_g000022 Rmu_sc0001226.1_g000016 Rmu_sc0002187.1_g000037 Rmu_sc0006084.1_g000005 Rmu_sc0006084.1_g000009 Rmu_sc0006084.1_g000019 Rmu_sc0006084.1_g000021
rosa_roxburghii	Rroxscaffold_1G00001540 Rroxscaffold_1G00003210 Rroxscaffold_1G00003250 Rroxscaffold_1G00003340 Rroxscaffold_1G00003580 Rroxscaffold_1G00003940 Rroxscaffold_3G00228660 Rroxscaffold_3G00247850
rosa_rugosa	Rorug05G0465800 Rorug05G0466100 Rorug07G0125700 Rorug07G0136300
rosa_wichuraiana	Rw0G005160 Rw0G022760 Rw5G047710 Rw5G048070 Rw5G048080 Rw5G048150 Rw5G048180 Rw5G048210 Rw5G048250 Rw5G048290 Rw7G023250 Rw7G030960

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3369
AatII	GACGTC	1 cut(s)	1551
Acc36I	ACCTGC	1 cut(s)	2212
AccB1I	GGYRCC	1 cut(s)	3021
AccBSI	CCGCTC	1 cut(s)	3019
AccI	GTMKAC	3 cut(s)	118, 654, 1165
AccII	CGCG	2 cut(s)	241, 2408
AciI	CCGC	9 cut(s)	456, 529, 561, 666, 843, 1908, 2043, 2408, 3019
AclWI	GGATC	5 cut(s)	418, 960, 1651, 1762, 1821
AcoI	YGGCCR	1 cut(s)	1634
AcuI	CTGAAG	2 cut(s)	1964, 2708
AcyI	GRCGYC	2 cut(s)	807, 1548
AdeI	CACNNNGTG	1 cut(s)	517
AfaI	GTAC	7 cut(s)	540, 724, 1487, 2546, 2742, 3133, 3317
AfiI	CCNNNNNNNGG	4 cut(s)	469, 566, 2022, 3031
AflII	CTTAAG	2 cut(s)	2639, 2714
AflIII	ACRYGT	1 cut(s)	694
AhdI	GACNNNNNGTC	1 cut(s)	2709
AjnI	CCWGG	1 cut(s)	1978
AjuI	GAANNNNNNNTTGG	6 cut(s)	1014, 1046, 1328, 1360, 2787, 2819
Alw26I	GTCTC	7 cut(s)	467, 685, 818, 1862, 2304, 2679, 2993
AlwI	GGATC	5 cut(s)	418, 960, 1651, 1762, 1821
AoxI	GGCC	5 cut(s)	3, 1116, 1284, 1634, 2265
ApeKI	GCWGC	9 cut(s)	437, 701, 746, 850, 1232, 1696, 2445, 2953, 3279
Asp700I	GAANNNNTTC	2 cut(s)	204, 1029
AspS9I	GGNCC	3 cut(s)	77, 2588, 2891
AsuC2I	CCSGG	2 cut(s)	1556, 3187
AsuHPI	GGTGA	5 cut(s)	433, 700, 973, 2422, 2669
AsuII	TTCGAA	2 cut(s)	1290, 2581
AvaII	GGWCC	3 cut(s)	77, 2588, 2891
BaeGI	GKGCMC	2 cut(s)	1198, 1596
BalI	TGGCCA	1 cut(s)	1636
BanI	GGYRCC	1 cut(s)	3021
BanII	GRGCYC	2 cut(s)	345, 561
BauI	CACGAG	1 cut(s)	2901
BbsI	GAAGAC	4 cut(s)	1246, 1377, 3009, 3071
BbvI	GCAGC	9 cut(s)	424, 688, 733, 837, 1244, 1683, 2432, 2940, 3291
BccI	CCATC	6 cut(s)	1268, 1520, 1583, 2080, 2802, 3233
BceAI	ACGGC	3 cut(s)	629, 673, 3009
BciT130I	CCWGG	1 cut(s)	1980
BciVI	GTATCC	1 cut(s)	1552
BcnI	CCSGG	2 cut(s)	1556, 3187
BcoDI	GTCTC	7 cut(s)	467, 685, 818, 1862, 2304, 2679, 2993
BfaI	CTAG	8 cut(s)	846, 1244, 1388, 1500, 1827, 2432, 2759, 2768
BfmI	CTRYAG	3 cut(s)	122, 769, 1929
BfrI	CTTAAG	2 cut(s)	2639, 2714
BfuAI	ACCTGC	1 cut(s)	2212
BfuI	GTATCC	1 cut(s)	1552
BglII	AGATCT	2 cut(s)	160, 285
Bme1390I	CCNGG	3 cut(s)	1556, 1980, 3187
Bme18I	GGWCC	3 cut(s)	77, 2588, 2891
BmeRI	GACNNNNNGTC	1 cut(s)	2709
BmgT120I	GGNCC	3 cut(s)	77, 2588, 2891
BmiI	GGNNCC	3 cut(s)	78, 342, 3023
BmrFI	CCNGG	3 cut(s)	1556, 1980, 3187
BmrI	ACTGGG	1 cut(s)	3284
BmsI	GCATC	8 cut(s)	166, 538, 1426, 1530, 2515, 2530, 2626, 2838
BmuI	ACTGGG	1 cut(s)	3284
BpiI	GAAGAC	4 cut(s)	1246, 1377, 3009, 3071
BplI	GAGNNNNNCTC	2 cut(s)	864, 896
BpmI	CTGGAG	1 cut(s)	1712
Bpu10I	CCTNAGC	1 cut(s)	390
Bpu14I	TTCGAA	2 cut(s)	1290, 2581
BpuEI	CTTGAG	1 cut(s)	2486
BpuMI	CCSGG	2 cut(s)	1556, 3187
BsaAI	YACGTR	1 cut(s)	3329
BsaBI	GATNNNNATC	1 cut(s)	159
BsaHI	GRCGYC	2 cut(s)	807, 1548
BsaI	GGTCTC	1 cut(s)	2679
BsaJI	CCNNGG	6 cut(s)	463, 600, 1011, 1979, 2482, 3185
BsaXI	ACNNNNNCTCC	2 cut(s)	2046, 2076
Bsc4I	CCNNNNNNNGG	4 cut(s)	469, 566, 2022, 3031
Bse1I	ACTGG	2 cut(s)	2591, 3290
Bse3DI	GCAATG	1 cut(s)	1170
Bse8I	GATNNNNATC	1 cut(s)	159
BseBI	CCWGG	1 cut(s)	1980
BseDI	CCNNGG	6 cut(s)	463, 600, 1011, 1979, 2482, 3185
BseJI	GATNNNNATC	1 cut(s)	159
BseLI	CCNNNNNNNGG	4 cut(s)	469, 566, 2022, 3031
BseMI	GCAATG	1 cut(s)	1170
BseMII	CTCAG	6 cut(s)	381, 1067, 1213, 2111, 2831, 3354
BseNI	ACTGG	2 cut(s)	2591, 3290
BseRI	GAGGAG	3 cut(s)	30, 382, 2624
BseSI	GKGCMC	2 cut(s)	1198, 1596
BseXI	GCAGC	9 cut(s)	424, 688, 733, 837, 1244, 1683, 2432, 2940, 3291
BseYI	CCCAGC	3 cut(s)	468, 938, 1613
BsgI	GTGCAG	3 cut(s)	687, 982, 2326
Bsh1236I	CGCG	2 cut(s)	241, 2408
BshFI	GGCC	5 cut(s)	5, 1118, 1286, 1636, 2267
BshNI	GGYRCC	1 cut(s)	3021
BsiSI	CCGG	4 cut(s)	188, 1149, 1555, 3187
BslFI	GGGAC	1 cut(s)	1429
BslI	CCNNNNNNNGG	4 cut(s)	469, 566, 2022, 3031
BsmAI	GTCTC	7 cut(s)	467, 685, 818, 1862, 2304, 2679, 2993
BsmBI	CGTCTC	1 cut(s)	467
BsmFI	GGGAC	1 cut(s)	1429
BsmI	GAATGC	2 cut(s)	1160, 1756
BsnI	GGCC	5 cut(s)	5, 1118, 1286, 1636, 2267
Bso31I	GGTCTC	1 cut(s)	2679
Bsp119I	TTCGAA	2 cut(s)	1290, 2581
Bsp1286I	GDGCHC	4 cut(s)	345, 561, 1198, 1596
Bsp1407I	TGTACA	1 cut(s)	2740
Bsp19I	CCATGG	3 cut(s)	600, 1011, 2482
Bsp68I	TCGCGA	1 cut(s)	241
BspACI	CCGC	9 cut(s)	456, 529, 561, 666, 843, 1908, 2043, 2408, 3019
BspANI	GGCC	5 cut(s)	5, 1118, 1286, 1636, 2267
BspCNI	CTCAG	6 cut(s)	382, 1068, 1212, 2112, 2832, 3353
BspFNI	CGCG	2 cut(s)	241, 2408
BspHI	TCATGA	1 cut(s)	2185
BspLI	GGNNCC	3 cut(s)	78, 342, 3023
BspMAI	CTGCAG	1 cut(s)	126
BspMI	ACCTGC	1 cut(s)	2212
BspPI	GGATC	5 cut(s)	418, 960, 1651, 1762, 1821
BspQI	GCTCTTC	2 cut(s)	832, 2870
BspT104I	TTCGAA	2 cut(s)	1290, 2581
BspT107I	GGYRCC	1 cut(s)	3021
BspTI	CTTAAG	2 cut(s)	2639, 2714
BspTNI	GGTCTC	1 cut(s)	2679
BsrBI	CCGCTC	1 cut(s)	3019
BsrDI	GCAATG	1 cut(s)	1170
BsrGI	TGTACA	1 cut(s)	2740
BsrI	ACTGG	2 cut(s)	2591, 3290
BssECI	CCNNGG	6 cut(s)	463, 600, 1011, 1979, 2482, 3185
BssNI	GRCGYC	2 cut(s)	807, 1548
BssSI	CACGAG	1 cut(s)	2901
BssT1I	CCWWGG	4 cut(s)	463, 600, 1011, 2482
Bst2BI	CACGAG	1 cut(s)	2901
Bst2UI	CCWGG	1 cut(s)	1980
Bst4CI	ACNGT	7 cut(s)	116, 921, 1267, 2112, 2942, 3070, 3136
Bst6I	CTCTTC	9 cut(s)	16, 305, 359, 816, 832, 865, 1701, 2684, 2870
BstACI	GRCGYC	2 cut(s)	807, 1548
BstAFI	CTTAAG	2 cut(s)	2639, 2714
BstAUI	TGTACA	1 cut(s)	2740
BstBAI	YACGTR	1 cut(s)	3329
BstBI	TTCGAA	2 cut(s)	1290, 2581
BstC8I	GCNNGC	4 cut(s)	561, 712, 1505, 1694
BstDSI	CCRYGG	3 cut(s)	600, 1011, 2482
BstFNI	CGCG	2 cut(s)	241, 2408
BstMAI	GTCTC	7 cut(s)	467, 685, 818, 1862, 2304, 2679, 2993
BstMWI	GCNNNNNNNGC	6 cut(s)	257, 400, 666, 707, 1091, 1181
BstNI	CCWGG	1 cut(s)	1980
BstNSI	RCATGY	2 cut(s)	698, 2973
BstSCI	CCNGG	3 cut(s)	1554, 1978, 3185
BstSFI	CTRYAG	3 cut(s)	122, 769, 1929
BstSLI	GKGCMC	2 cut(s)	1198, 1596
BstUI	CGCG	2 cut(s)	241, 2408
BstV1I	GCAGC	9 cut(s)	424, 688, 733, 837, 1244, 1683, 2432, 2940, 3291
BstV2I	GAAGAC	4 cut(s)	1246, 1377, 3009, 3071
BstX2I	RGATCY	5 cut(s)	160, 285, 965, 1767, 1813
BstYI	RGATCY	5 cut(s)	160, 285, 965, 1767, 1813
BsuI	GTATCC	1 cut(s)	1552
BsuRI	GGCC	5 cut(s)	5, 1118, 1286, 1636, 2267
BtgI	CCRYGG	3 cut(s)	600, 1011, 2482
BtsI	GCAGTG	4 cut(s)	131, 434, 639, 3057
BtsIMutI	CAGTG	8 cut(s)	131, 434, 639, 1482, 2044, 2598, 2877, 3057
BtuMI	TCGCGA	1 cut(s)	241
BveI	ACCTGC	1 cut(s)	2212
Cac8I	GCNNGC	4 cut(s)	561, 712, 1505, 1694
CciI	TCATGA	1 cut(s)	2185
Cfr13I	GGNCC	3 cut(s)	77, 2588, 2891
CseI	GACGC	2 cut(s)	58, 796
Csp6I	GTAC	7 cut(s)	539, 723, 1486, 2545, 2741, 3132, 3316
CviQI	GTAC	7 cut(s)	539, 723, 1486, 2545, 2741, 3132, 3316
DraI	TTTAAA	5 cut(s)	444, 1626, 1761, 2329, 2775
DraIII	CACNNNGTG	1 cut(s)	517
DriI	GACNNNNNGTC	1 cut(s)	2709
EaeI	YGGCCR	1 cut(s)	1634
Eam1104I	CTCTTC	9 cut(s)	16, 305, 359, 816, 832, 865, 1701, 2684, 2870
Eam1105I	GACNNNNNGTC	1 cut(s)	2709
EarI	CTCTTC	9 cut(s)	16, 305, 359, 816, 832, 865, 1701, 2684, 2870
Eco130I	CCWWGG	4 cut(s)	463, 600, 1011, 2482
Eco147I	AGGCCT	1 cut(s)	1286
Eco24I	GRGCYC	2 cut(s)	345, 561
Eco31I	GGTCTC	1 cut(s)	2679
Eco32I	GATATC	2 cut(s)	359, 2085
Eco47I	GGWCC	3 cut(s)	77, 2588, 2891
Eco57I	CTGAAG	2 cut(s)	1964, 2708
EcoO109I	RGGNCCY	1 cut(s)	77
EcoRI	GAATTC	1 cut(s)	933
EcoRII	CCWGG	1 cut(s)	1978
EcoRV	GATATC	2 cut(s)	359, 2085
EcoT14I	CCWWGG	4 cut(s)	463, 600, 1011, 2482
EcoT22I	ATGCAT	2 cut(s)	1545, 2853
EcoT38I	GRGCYC	2 cut(s)	345, 561
ErhI	CCWWGG	4 cut(s)	463, 600, 1011, 2482
Esp3I	CGTCTC	1 cut(s)	467
FalI	AAGNNNNNCTT	4 cut(s)	528, 560, 1328, 1360
FaqI	GGGAC	1 cut(s)	1429
FauI	CCCGC	2 cut(s)	568, 1901
FauNDI	CATATG	1 cut(s)	2700
FblI	GTMKAC	3 cut(s)	118, 654, 1165
FriOI	GRGCYC	2 cut(s)	345, 561
FspBI	CTAG	8 cut(s)	846, 1244, 1388, 1500, 1827, 2432, 2759, 2768
GsaI	CCCAGC	3 cut(s)	472, 942, 1617
GsuI	CTGGAG	1 cut(s)	1712
HaeIII	GGCC	5 cut(s)	5, 1118, 1286, 1636, 2267
HapII	CCGG	4 cut(s)	188, 1149, 1555, 3187
HgaI	GACGC	2 cut(s)	58, 796
Hin1I	GRCGYC	2 cut(s)	807, 1548
HincII	GTYRAC	2 cut(s)	119, 924
HindII	GTYRAC	2 cut(s)	119, 924
HindIII	AAGCTT	4 cut(s)	381, 1875, 1949, 2926
HpaII	CCGG	4 cut(s)	188, 1149, 1555, 3187
HphI	GGTGA	5 cut(s)	433, 700, 973, 2422, 2669
Hpy166II	GTNNAC	7 cut(s)	119, 655, 895, 924, 1166, 2242, 3055
Hpy8I	GTNNAC	7 cut(s)	119, 655, 895, 924, 1166, 2242, 3055
Hpy99I	CGWCG	2 cut(s)	120, 1434
HpyCH4III	ACNGT	7 cut(s)	116, 921, 1267, 2112, 2942, 3070, 3136
HpyCH4IV	ACGT	2 cut(s)	1548, 3328
HpyF10VI	GCNNNNNNNGC	6 cut(s)	257, 400, 666, 707, 1091, 1181
HpySE526I	ACGT	2 cut(s)	1548, 3328
Hsp92I	GRCGYC	2 cut(s)	807, 1548
LguI	GCTCTTC	2 cut(s)	832, 2870
LmnI	GCTCC	3 cut(s)	43, 340, 949
Lsp1109I	GCAGC	9 cut(s)	424, 688, 733, 837, 1244, 1683, 2432, 2940, 3291
LweI	GCATC	8 cut(s)	166, 538, 1426, 1530, 2515, 2530, 2626, 2838
MaeI	CTAG	8 cut(s)	846, 1244, 1388, 1500, 1827, 2432, 2759, 2768
MaeII	ACGT	2 cut(s)	1548, 3328
MaeIII	GTNAC	7 cut(s)	451, 2062, 2410, 2459, 2490, 2661, 3329
MbiI	CCGCTC	1 cut(s)	3019
MfeI	CAATTG	3 cut(s)	225, 552, 2996
MflI	RGATCY	5 cut(s)	160, 285, 965, 1767, 1813
MhlI	GDGCHC	4 cut(s)	345, 561, 1198, 1596
MlsI	TGGCCA	1 cut(s)	1636
MluNI	TGGCCA	1 cut(s)	1636
MlyI	GAGTC	2 cut(s)	484, 2926
MmeI	TCCRAC	4 cut(s)	652, 1408, 3012, 3319
Mox20I	TGGCCA	1 cut(s)	1636
Mph1103I	ATGCAT	2 cut(s)	1545, 2853
MroXI	GAANNNNTTC	2 cut(s)	204, 1029
MscI	TGGCCA	1 cut(s)	1636
MslI	CAYNNNNRTG	1 cut(s)	2468
Msp20I	TGGCCA	1 cut(s)	1636
MspA1I	CMGCKG	1 cut(s)	1235
MspCI	CTTAAG	2 cut(s)	2639, 2714
MspI	CCGG	4 cut(s)	188, 1149, 1555, 3187
MspR9I	CCNGG	3 cut(s)	1556, 1980, 3187
MunI	CAATTG	3 cut(s)	225, 552, 2996
Mva1269I	GAATGC	2 cut(s)	1160, 1756
MvaI	CCWGG	1 cut(s)	1980
MvnI	CGCG	2 cut(s)	241, 2408
MwoI	GCNNNNNNNGC	6 cut(s)	257, 400, 666, 707, 1091, 1181
NciI	CCSGG	2 cut(s)	1556, 3187
NcoI	CCATGG	3 cut(s)	600, 1011, 2482
NdeI	CATATG	1 cut(s)	2700
NlaIV	GGNNCC	3 cut(s)	78, 342, 3023
NmeAIII	GCCGAG	2 cut(s)	2600, 3007
NmuCI	GTSAC	5 cut(s)	451, 2062, 2410, 2459, 3329
NruI	TCGCGA	1 cut(s)	241
NsiI	ATGCAT	2 cut(s)	1545, 2853
NspI	RCATGY	2 cut(s)	698, 2973
NspV	TTCGAA	2 cut(s)	1290, 2581
PagI	TCATGA	1 cut(s)	2185
PceI	AGGCCT	1 cut(s)	1286
PciI	ACATGT	1 cut(s)	694
PciSI	GCTCTTC	2 cut(s)	832, 2870
PctI	GAATGC	2 cut(s)	1160, 1756
PdmI	GAANNNNTTC	2 cut(s)	204, 1029
PfoI	TCCNGGA	1 cut(s)	1554
PleI	GAGTC	2 cut(s)	484, 2925
PpsI	GAGTC	2 cut(s)	484, 2925
Ppu21I	YACGTR	1 cut(s)	3329
PpuMI	RGGWCCY	1 cut(s)	77
PscI	ACATGT	1 cut(s)	694
PsiI	TTATAA	1 cut(s)	3369
Psp5II	RGGWCCY	1 cut(s)	77
Psp6I	CCWGG	1 cut(s)	1978
PspFI	CCCAGC	3 cut(s)	468, 938, 1613
PspGI	CCWGG	1 cut(s)	1978
PspN4I	GGNNCC	3 cut(s)	78, 342, 3023
PspPI	GGNCC	3 cut(s)	77, 2588, 2891
PspPPI	RGGWCCY	1 cut(s)	77
PsrI	GAACNNNNNNTAC	2 cut(s)	2100, 2132
PstI	CTGCAG	1 cut(s)	126
PsuI	RGATCY	5 cut(s)	160, 285, 965, 1767, 1813
PvuII	CAGCTG	1 cut(s)	1235
RruI	TCGCGA	1 cut(s)	241
RsaI	GTAC	7 cut(s)	540, 724, 1487, 2546, 2742, 3133, 3317
RsaNI	GTAC	7 cut(s)	539, 723, 1486, 2545, 2741, 3132, 3316
RseI	CAYNNNNRTG	1 cut(s)	2468
SalI	GTCGAC	1 cut(s)	117
SapI	GCTCTTC	2 cut(s)	832, 2870
Sau96I	GGNCC	3 cut(s)	77, 2588, 2891
SchI	GAGTC	2 cut(s)	484, 2926
ScrFI	CCNGG	3 cut(s)	1556, 1980, 3187
SduI	GDGCHC	4 cut(s)	345, 561, 1198, 1596
SfaNI	GCATC	8 cut(s)	166, 538, 1426, 1530, 2515, 2530, 2626, 2838
SfcI	CTRYAG	3 cut(s)	122, 769, 1929
SfuI	TTCGAA	2 cut(s)	1290, 2581
SinI	GGWCC	3 cut(s)	77, 2588, 2891
SmiI	ATTTAAAT	2 cut(s)	1761, 2775
SmiMI	CAYNNNNRTG	1 cut(s)	2468
SmlI	CTYRAG	3 cut(s)	2501, 2639, 2714
SmoI	CTYRAG	3 cut(s)	2501, 2639, 2714
SseBI	AGGCCT	1 cut(s)	1286
SsiI	CCGC	9 cut(s)	456, 529, 561, 666, 843, 1908, 2043, 2408, 3019
SspI	AATATT	3 cut(s)	1573, 2179, 2217
SspMI	CTAG	8 cut(s)	846, 1244, 1388, 1500, 1827, 2432, 2759, 2768
StuI	AGGCCT	1 cut(s)	1286
StyD4I	CCNGG	3 cut(s)	1554, 1978, 3185
StyI	CCWWGG	4 cut(s)	463, 600, 1011, 2482
SwaI	ATTTAAAT	2 cut(s)	1761, 2775
TaaI	ACNGT	7 cut(s)	116, 921, 1267, 2112, 2942, 3070, 3136
TaiI	ACGT	2 cut(s)	1551, 3331
TaqI	TCGA	6 cut(s)	118, 157, 1290, 1429, 1916, 2581
TatI	WGTACW	1 cut(s)	2740
TauI	GCSGC	2 cut(s)	669, 845
TscAI	CASTG	8 cut(s)	131, 434, 646, 1482, 2044, 2598, 2877, 3057
TseFI	GTSAC	5 cut(s)	451, 2062, 2410, 2459, 3329
TseI	GCWGC	9 cut(s)	437, 701, 746, 850, 1232, 1696, 2445, 2953, 3279
Tsp45I	GTSAC	5 cut(s)	451, 2062, 2410, 2459, 3329
TspGWI	ACGGA	3 cut(s)	423, 1003, 1825
TspRI	CASTG	8 cut(s)	131, 434, 646, 1482, 2044, 2598, 2877, 3057
Vha464I	CTTAAG	2 cut(s)	2639, 2714
VpaK11BI	GGWCC	3 cut(s)	77, 2588, 2891
XbaI	TCTAGA	1 cut(s)	2758
XceI	RCATGY	2 cut(s)	698, 2973
XmiI	GTMKAC	3 cut(s)	118, 654, 1165
XmnI	GAANNNNTTC	2 cut(s)	204, 1029
XspI	CTAG	8 cut(s)	846, 1244, 1388, 1500, 1827, 2432, 2759, 2768
ZraI	GACGTC	1 cut(s)	1549
Zsp2I	ATGCAT	2 cut(s)	1545, 2853

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.